Plant Signaling & Behavior最新文献_第8页

Evaluation of the biocontrol activity of Frateuria defendens-derived metabolites against mollicutes 防御菌衍生代谢物对分子物的生物防治活性评价

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-04-29 DOI: 10.1080/15592324.2022.2070355

Alaa Naama-Amar, Y. Gerchman, Lilach Iasur Kruh, V. Naor

{"title":"Evaluation of the biocontrol activity of Frateuria defendens-derived metabolites against mollicutes","authors":"Alaa Naama-Amar, Y. Gerchman, Lilach Iasur Kruh, V. Naor","doi":"10.1080/15592324.2022.2070355","DOIUrl":"https://doi.org/10.1080/15592324.2022.2070355","url":null,"abstract":"ABSTRACT Frateuria defendens is a candidate biocontrol agent that has been shown to reduce phytoplasma-related disease symptoms in grapevines and periwinkle plants. While a crude filtrate prepared from F. defendens can inhibit mollicute growth, the specific growth parameters for this bacterium, necessary to enhance this protective inhibitory response, remain unknown. Moreover, the separation of filtrate preparations from bacterial cells via centrifugation and filtration is laborious and time-consuming. As such, the present study was conducted to define the optimal growth conditions associated with maximal inhibitory activity of F. defendens and to establish a better approach to separating these bacterial cells from their secreted metabolites. To conduct these analyses, F. defendens was cultured in a range of media types, while associated inhibitory effects were tested in vitro using Spiroplasma melliferum as a model mollicute bacterium, and in planta using phytoplasma-infected periwinkle plantlets. These analyses revealed F. defendens growth patterns change based upon media composition, with filtrates prepared from a specific rich medium (S-medium) exhibiting beneficial activities, including the inhibition of S. melliferum and enhanced plant growth. When F. defendens cells were grown within semi-permeable, membrane-coated Small Bioreactor Platform (SBP) capsules, they could be more readily separated from the secreted metabolite fraction, obviating the need for filtration and/or centrifugation. This study is the first to have reported the use of SBP capsules to separate bacterial cells from their secreted metabolites under sterile conditions while retaining the ability of these metabolites to inhibit S. melliferum growth and to benefit the host plant. The results highlight promising new approaches to the effective biocontrol of phytoplasma-driven diseases in grapevines and other economically important plant species.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"56 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80456328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Nitric oxide (NO) involved in Cd tolerance in NHX1 transgenic duckweed during Cd stress 一氧化氮(NO)参与转NHX1基因浮萍在Cd胁迫下的Cd耐受

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-04-26 DOI: 10.1080/15592324.2022.2065114

Qiuting Ren, Na Li, Ruxin Liu, Xueman Ma, Jinge Sun, Jianyao Zeng, Qingqing Li, Mingwei Wang, Xinglin Chen, Xiaoyu Wu, Lin Yang

引用次数: 3

Functional redundancy of OsPIN1 paralogous genes in regulating plant growth and development in rice OsPIN1旁系基因在水稻生长发育调控中的功能冗余

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-04-20 DOI: 10.1080/15592324.2022.2065432

Yong Li, Lingling Wu, Meiyan Ren, Jianshu Zhu, Jiming Xu, Han Hu, Xiaokang Quan, Chongping Huang, C. Mao

{"title":"Functional redundancy of OsPIN1 paralogous genes in regulating plant growth and development in rice","authors":"Yong Li, Lingling Wu, Meiyan Ren, Jianshu Zhu, Jiming Xu, Han Hu, Xiaokang Quan, Chongping Huang, C. Mao","doi":"10.1080/15592324.2022.2065432","DOIUrl":"https://doi.org/10.1080/15592324.2022.2065432","url":null,"abstract":"ABSTRACT The OsPIN1 paralogous genes (OsPIN1a-1d) are important for root and panicle development in rice (Oryza sativa L.). However, the specific role of OsPIN1 paralogous genes is still not clear. To understand the specific roles of PIN1 paralogs in rice, we generated pin1 triple and quadruple mutants by crossing the pin1a pin1b and pin1c pin1d double mutants which we previously created. Compared with the 7-day-old wild type, the pin1a pin1c pin1d and pin1b pin1c pin1d triple mutants showed no obvious phenotype variation except that the pin1a pin1c pin1d triple mutant had shorter primary root and shoot. The pin1a pin1b pin1c and pin1a pin1b pin1d triple mutants exhibited a series of developmental abnormalities, including shorter primary roots, longer root hairs, fewer crown roots and lateral roots, shorter and curved shoots. Furthermore, the pin1a pin1b pin1c pin1d quadruple mutant displayed more severe phenotypic defects which was lethal. In addition, the expression levels of some hormone signal transduction and crown root development related genes, such as OsIAAs, OsARFs, OsRRs, and OsCRLs, were significantly altered in the stem base of all examined pin1 multiple mutants. Taken together, our results demonstrated that the four OsPIN1 paralogous genes function redundantly in regulating rice growth and development.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"16 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86126822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genome-wide identification and expression analysis of Raf-like kinase gene family in pepper (Capsicum annuum L.) 辣椒(Capsicum annuum L.) raf样激酶基因家族的全基因组鉴定与表达分析

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-04-17 DOI: 10.1080/15592324.2022.2064647

C. Lim, Sung Chul Lee

{"title":"Genome-wide identification and expression analysis of Raf-like kinase gene family in pepper (Capsicum annuum L.)","authors":"C. Lim, Sung Chul Lee","doi":"10.1080/15592324.2022.2064647","DOIUrl":"https://doi.org/10.1080/15592324.2022.2064647","url":null,"abstract":"ABSTRACT As highly conserved signaling pathway modules, mitogen-activated protein kinase (MAPK) cascades play vital roles in a diverse range of stress and hormonal responses in plants. Among the established components of MAPK cascades, Raf-like MAPK kinase kinases (MAPKKKs) are associated with abscisic acid (ABA) signaling and osmotic stress responses. However, despite the availability of a pepper reference genome, few of the Raf-like kinases in pepper plants have been functionally characterized. In this study, we isolated 47 putative Raf-like kinase genes from the pepper genome based on in silico analysis, which were classified into two major categories, namely, groups B and C (further sub-grouped into B1–B4 and C1–C7, respectively) and named sequentially as CaRaf1 to CaRaf47. Subcellular localization prediction analysis revealed that most of the group B CaRaf-like kinases are probably nuclear-localized, whereas a majority of group C members targeted into the cytoplasm. Transcriptional regulation of the 47 CaRaf genes in response to treatment with ABA, drought, NaCl, and mannitol was quantitatively analyzed by reverse-transcription PCR analysis. This revealed a significant induction of subgroup B3, C2, C3, and C5 members, indicating that these genes may be functionally associated with the response to osmotic stress, mediated via both ABA-dependent and -independent pathways. The findings of this study can accordingly serve as a basis for the identification of CaRaf genes associated with the regulation of ABA signaling and osmotic stress response and thus contribute to enhancing our understanding of the biological functions of CaRaf kinases in the responses of plants to different abiotic stresses.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"2015 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73825568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Overexpression of LpCPC from Lilium pumilum confers saline-alkali stress (NaHCO3) resistance 百合LpCPC的过表达使百合具有耐盐碱胁迫(NaHCO3)的能力

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-04-09 DOI: 10.1080/15592324.2022.2057723

Yi Dong, Ling Zhang, X. Chang, Xiaolu Wang, Guanrong Li, Shiyao Chen, Shumei Jin

{"title":"Overexpression of LpCPC from Lilium pumilum confers saline-alkali stress (NaHCO3) resistance","authors":"Yi Dong, Ling Zhang, X. Chang, Xiaolu Wang, Guanrong Li, Shiyao Chen, Shumei Jin","doi":"10.1080/15592324.2022.2057723","DOIUrl":"https://doi.org/10.1080/15592324.2022.2057723","url":null,"abstract":"ABSTRACT Lilium Pumilum with wide distribution is highly tolerant to salinity. The blue copper protein LpCPC (Lilium pumilum Cucumber Peeling Cupredoxin) gene was cloned from Lilium pumilum, which has the conserved regions of type I copper protein. Moreover, LpCPC has the closest relation to CPC from Actinidia chinensis using DNAMAN software and MEGA7 software. qRT-PCR indicated that LpCPC expression was higher in root and bulb of Lilium pumilum, and the expression of the LpCPC gene increased and reached the highest level at 12 h in bulbs under 20 mM NaHCO3. The transgenic yeast was more tolerant compared with the control under NaHCO3 stress. Compared with the wild type, overexpressing plants indicated a relatively lower degree of wilting. In addition, the chlorophyll content, soluble phenol content, and lignin content of overexpressing lines were higher than that of wild-type, whereas the relative conductivity of overexpressing plants was significantly lower than that of wild-type plants. Expression of essential genes including NHX1 and SOS1 in salt stress response pathways are steadily higher in overexpression tobacco than that in wild-types. Transgenic lines had much higher levels of CCR1 and CAD, which are involved in lignin production, compared with wild-type lines. The yeast two-hybrid technique was applied to screen probable interacting proteins interacting with LpCPC. Eight proteins interacted with LpCPC were screened, and five of which were demonstrated to be associated with plant salinity resistance. Overall, the role of gene LpCPC is mediating molecule responses in increasing saline-alkali stress resistance, indicating that it is an essential gene to enhance salt tolerance in Lilium pumilum.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"11 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80148746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Dried Nostoc commune exhibits nitrogen-fixing activity using glucose under dark conditions after rehydration 在复水化后的黑暗条件下，干燥的Nostoc公社表现出利用葡萄糖的固氮活性

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-04-06 DOI: 10.1080/15592324.2022.2059251

S. Hata, Shoji Kishida, Risa Minesono, T. Tamai

引用次数: 1

Allelopathic inhibition of the extracts of Landoltia punctata on Microcystis aeruginosa 斑点莲提取物对铜绿微囊藻的化感抑制作用

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-04-05 DOI: 10.1080/15592324.2022.2058256

Li Dan, Li Peng, Y. Zhiqiang, L. Na, Yao Lunguang, Cao Lingling

{"title":"Allelopathic inhibition of the extracts of Landoltia punctata on Microcystis aeruginosa","authors":"Li Dan, Li Peng, Y. Zhiqiang, L. Na, Yao Lunguang, Cao Lingling","doi":"10.1080/15592324.2022.2058256","DOIUrl":"https://doi.org/10.1080/15592324.2022.2058256","url":null,"abstract":"ABSTRACT To study the allelopathic effect of the extracts of Landoltia punctata, the changes of cell density of Microcystis aeruginosa were measured. The anti-algae allelopathic effect of different organic solvent extracts of L. punctata was evaluated, and the physiological, biochemical indexes were determined to discuss the mechanism of algal inhibition. The results showed that the petroleum ether, dichloromethane and ethyl acetate extracts showed various inhibitory effects on M. aeruginosa. Among them, ethyl acetate extract was the most strongly allelopathic part with the semi-effect concentration(EC50) of 59.6 mg L−1, the central polarity part of inhibitory activity. The contents of chlorophyll a(Chl a) and phycobiliproteins(PBPs) of M. aeruginosa were decreased under the concentration of 200 mg L−1 ethyl acetate extract, which indicated that the photosynthesis of M. aeruginosa was inhibited. The consent of microcystins was lower compared to control under 200 mg L−1. The contents of superoxide dismutase(SOD), malondialdehyde(MDA) and hydrogen peroxide(H2O2) of cell pellets were firstly increased and then decreased, which suggested that the algal cells were seriously damaged by oxidation. The results indicated that the extracts of L. punctata had inhibitory effect on M. aeruginosa, and the ethyl acetate extract was the central part of the inhibitory substances, which affected photosynthesis and caused peroxidation damage to inhibit cell proliferation. These findings will be helpful for exploration and application of allelopathic effects of L. punctata in harmful algae control.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79922118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

GIGANTEA regulates PAD4 transcription to promote pathogen defense against Hyaloperonospora arabidopsidis in Arabidopsis thaliana GIGANTEA调节PAD4转录促进拟南芥对拟南芥透明膜孢子虫的病原体防御

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-04-04 DOI: 10.1080/15592324.2022.2058719

Anamika Singh

{"title":"GIGANTEA regulates PAD4 transcription to promote pathogen defense against Hyaloperonospora arabidopsidis in Arabidopsis thaliana","authors":"Anamika Singh","doi":"10.1080/15592324.2022.2058719","DOIUrl":"https://doi.org/10.1080/15592324.2022.2058719","url":null,"abstract":"ABSTRACT Plants have evolved a network of complex signaling pathways that allow them to cope with the fluctuations of internal and external environmental cues. GIGANTEA (GI), a well-known, highly conserved plant nuclear protein, has been shown to regulate multiple biological functions in plants such as circadian rhythm, light signaling, cold tolerance, hormone signaling, and photoperiodic flowering. Recently, the role of GI in disease tolerance against different pathogens has come to light; however, a detailed mechanism to understand the role of GI in pathogen defense remains largely unexplained. Here, we report that GIGANTEA is upregulated upon infection with a virulent oomycete pathogen, Hyaloperonospora arabidopsidis (Hpa), in Arabidopsis thaliana accession Col-0. To investigate the role of GI in Arabidopsis defense, we examined the pathogen infection phenotype of gi mutant plants and found that gi-100 mutant was highly susceptible to Hpa Noco2 infection. Notably, the quantitative real-time PCR showed that PHYTOALEXIN DEFICIENT4 (PAD4) and several PAD4-regulated downstream genes were downregulated upon Noco2 infection in gi-100 mutant as compared to Col-0 plants. Furthermore, the chromatin immunoprecipitation results show that GI can directly bind to the intronic region of the PAD4 gene, which might explain the mechanism of GI function in regulating disease resistance in plants. Taken together, our results suggest that GI expression is induced upon Hpa pathogen infection and GI can regulate the expression of PAD4 to promote resistance against the oomycete pathogen Hyaloperonospora arabidopsidis in Arabidopsis thaliana.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"23 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89037550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Overexpression of the WOX5 gene inhibits shoot development 过表达WOX5基因会抑制茎的发育

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-04-01 DOI: 10.1080/15592324.2022.2050095

Kyounghee Lee, Jin Hoon Won, P. Seo

引用次数: 2

Simultaneous mutations in SMN1 and SUMM2 fully suppress the dwarf and autoimmune phenotypes of Arabidopsis mpk4 mutant SMN1和SUMM2的同时突变完全抑制拟南芥mpk4突变体的矮化和自身免疫表型

IF 2.9 4区生物学

Plant Signaling & Behavior Pub Date : 2022-03-30 DOI: 10.1080/15592324.2022.2046412

Momoko Takagi, Suzuna Nagai, H. Kaminaka, K. Akimitsu, K. Shirasu, K. Ichimura

{"title":"Simultaneous mutations in SMN1 and SUMM2 fully suppress the dwarf and autoimmune phenotypes of Arabidopsis mpk4 mutant","authors":"Momoko Takagi, Suzuna Nagai, H. Kaminaka, K. Akimitsu, K. Shirasu, K. Ichimura","doi":"10.1080/15592324.2022.2046412","DOIUrl":"https://doi.org/10.1080/15592324.2022.2046412","url":null,"abstract":"ABSTRACT Disruption of the Arabidopsis mitogen-activated protein kinase pathway, MEKK1–MKK1/MKK2–MPK4 (hereafter designated as MEKK1 pathway), leads to the activation of distinct NLRs (nucleotide-binding and leucine-rich repeat receptors), TNL (TIR-type NLR) SMN1, and CNL (CC-type NLR) SUMM2, resulting in dwarf and autoimmune phenotypes. Unlike mekk1 and mkk1mkk2 mutants, the dwarf and autoimmune phenotypes of mpk4 are only partially suppressed by the summ2 mutation, suggesting a significant contribution of SMN1 to the mpk4 phenotypes. However, full suppression of mpk4 by the smn1summ2 double mutation remains to be elucidated. To address this key question, we generated a mpk4smn1summ2 triple mutant and analyzed the dwarf and constitutive cell death phenotypes. The mpk4smn1summ2 triple mutant showed restoration of plant size with no detectable cell death, indicating full suppression of the dwarf and autoimmune phenotypes. These results suggest that SMN1 and SUMM2 constitute a robust surveillance system for the MEKK1 pathway against pathogen infection.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"63 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74542303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3