Mechanism of calcium signal response to cadmium stress in duckweed.

IF 2.8 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Signaling & Behavior Pub Date : 2022-12-31 DOI:10.1080/15592324.2022.2119340

Qiuting Ren, Ziyi Xu, Ying Xue, Rui Yang, Xu Ma, Jinge Sun, Jing Wang, Shuang Lin, Wenqiao Wang, Lin Yang, Zhanpeng Sun

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引用次数: 3

Abstract

Cadmium (Cd) causes serious damage to plants. Although calcium (Ca) signal has been found to respond to certain stress, the localization of Ca and molecular mechanisms underlying Ca signal in plants during Cd stress are largely unknown. In this study, Ca²⁺-sensing fluorescent reporter (GCaMP3) transgenic duckweed showed the Ca²⁺ signal response in Lemna turionifera 5511 (duckweed) during Cd stress. Subsequently, the subcellular localization of Ca²⁺ has been studied during Cd stress by transmission electron microscopy, showing the accumulation of Ca²⁺ in vacuoles. Also, Ca²⁺ flow during Cd stress has been measured. At the same time, the effects of exogenous glutamic acid (Glu) and γ-aminobutyric (GABA) on duckweed can better clarify the signal operation mechanism of plants to Cd stress. The molecular mechanism of Ca²⁺ signal responsed during Cd stress showed that Cd treatment promotes the positive response of Ca signaling channels in plant cells, and thus affects the intracellular Ca content. These novel signal studies provided an important Ca²⁺ signal molecular mechanism during Cd stress.

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浮萍钙信号对镉胁迫的响应机制。

镉(Cd)对植物造成严重危害。虽然已经发现钙(Ca)信号对某些胁迫有响应，但Cd胁迫下植物体内钙的定位和钙信号的分子机制仍不清楚。在本研究中，转Ca2+感应荧光报告基因(GCaMP3)的浮萍在Cd胁迫下显示了lena turionifera 5511对Ca2+信号的响应。随后，通过透射电镜研究了镉胁迫下Ca2+的亚细胞定位，显示了Ca2+在液泡中的积累。还测量了镉胁迫下Ca2+的流动。同时，外源谷氨酸(Glu)和γ-氨基丁酸(GABA)对浮萍的影响可以更好地阐明植物对Cd胁迫的信号操作机制。Cd胁迫下Ca2+信号响应的分子机制表明，Cd处理促进了植物细胞内Ca信号通道的积极响应，从而影响细胞内Ca含量。这些新的信号研究提供了镉胁迫下Ca2+信号的重要分子机制。

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来源期刊

Plant Signaling & Behavior Agricultural and Biological Sciences-Plant Science

CiteScore

6.00

自引率

3.40%

发文量

111

期刊介绍： Plant Signaling & Behavior, a multidisciplinary peer-reviewed journal published monthly online, publishes original research articles and reviews covering the latest aspects of signal perception and transduction, integrative plant physiology, and information acquisition and processing.