Plant Signaling & Behavior最新文献

{"title":"Activation of drought tolerant traits in crops: endophytes as elicitors.","authors":"Karaba N Nataraja,&nbsp;K H Dhanyalakshmi,&nbsp;Geetha Govind,&nbsp;Ralf Oelmüller","doi":"10.1080/15592324.2022.2120300","DOIUrl":"https://doi.org/10.1080/15592324.2022.2120300","url":null,"abstract":"<p><p>Drought challenges crop production worldwide. The issue is aggravated by frequent drought episodes and unpredictable rainfall patterns associated with global climate change. While the efforts to breed drought-resistant crop varieties are progressing, the need of the hour is immediate strategies to sustain the yields of existing ones. As per recent studies, stress adaptive traits can be activated using specific elicitors. Endophytes that inhabit host plants asymptomatically are natural elicitors/bio-stimulators capable of activating host gene expression, conferring several benefits to the hosts. This review discusses the scope of using trait-specific endophytes in activating drought adaptive traits in crop varieties.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2120300"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9665085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10787941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SCaBP3/CBL7 negatively regulates the plasma membrane H⁺-ATPase and modulates hypocotyl elongation in <i>Arabidopsis</i>.","authors":"Xiao Liu,&nbsp;Yujiao Wu,&nbsp;Haiqi Fu,&nbsp;Shu Song,&nbsp;Qian He,&nbsp;Yongqing Yang","doi":"10.1080/15592324.2022.2092699","DOIUrl":"https://doi.org/10.1080/15592324.2022.2092699","url":null,"abstract":"<p><p>The regulation of hypocotyl elongation is an important process in plant growth and development and depends on the activity of the plasma membrane (PM) H⁺-ATPase. In this study, we found that the Arabidopsis protein SOS3-LIKE CALCIUM BINDING PROTEIN3 (SCaBP3) negatively regulates PM H⁺-ATPase activity in yeast and hypocotyl elongation in dark-grown seedlings. Yeast two-hybrid assays showed that SCaBP3 interacts with representative members of the Arabidopsis PM H⁺-ATPase family. Experiments in RS-72 yeast showed that SCaBP3 negatively regulates PM H⁺-ATPase activity-dependent yeast cell growth. Hypocotyl elongation was promoted in the loss-of-function mutant <i>scabp3</i> and inhibited in <i>SCaBP3</i> overexpression lines of Arabidopsis. We propose that SCaBP3 modulates hypocotyl elongation by negatively regulating PM H⁺-ATPase activity.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2092699"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40406602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monoubiquitinated MxIRT1 acts as an iron receptor to determine MxIRT1 vacuole degradation or plasma membrane recycling via endocytosis.","authors":"Song Tan,&nbsp;Shuang Li,&nbsp;Xiu-Yue Zhang,&nbsp;Yu-Meng Li,&nbsp;Peng Zhang,&nbsp;Li-Ping Yin","doi":"10.1080/15592324.2022.2095141","DOIUrl":"https://doi.org/10.1080/15592324.2022.2095141","url":null,"abstract":"<p><p>IRON-REGULATED TRANSPORTER 1 (IRT1) is critical for iron uptake in roots, and its exocytosis to the plasma membrane (PM) is regulated by the iron status sensed by the histidine-rich domain (HRM). However, studies on the fate of IRT1 after fusion with PM in response to iron conditions are still limited. In this study, we found that K165 and K196 regulate the monoubiquitination of MxIRT1 (mUb-MxIRT1), which acts as a receptor delivering signals from HRM to downstream effectors such as clathrin to determine the fate of MxIRT1. Iron supply led MxIRT1 in the PM to monoubiquitin-dependent endocytosis which could be inhibited by endocytosis inhibitor TyrA23 or in the double site-directed mutant K165/K196R. Subsequently, the endocytosis pathway to the vacuole was inhibited by vacuolar protease inhibitor Leupeptin in excessive iron conditions and the inability of being able to respond to iron change, indicated by the protein accumulating in the PM, contributed to iron toxicity in K165/K196R transgenic Arabidopsis. With iron availability decreasing again, MxIRT1 could dock close to the PM waiting for to be recycled. Another monoubiquitination site, K26, was necessary for MxIRT1 Endoplasmic Reticulum (ER) export as site-directed mutant K26R lost the ability of PM targeting, and co-localized with the COPII subunit of the coat protein OsSec24. Therefore, after K26-directed ER export and iron-induced PM fusion, mUb-MxIRT1 determines subsequent vacuolar degradation or recycling to the PM via endocytosis for maintaining iron homeostasis.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2095141"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9255258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40468760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pseudomonas fluorescens</i> BsEB-1: an endophytic bacterium isolated from the root of <i>Bletilla striata</i> that can promote its growth.","authors":"Yuanshuang Wu,&nbsp;Suhui Xiao,&nbsp;Jiaseng Qi,&nbsp;Yongchang Gong,&nbsp;Kunzhi Li","doi":"10.1080/15592324.2022.2100626","DOIUrl":"https://doi.org/10.1080/15592324.2022.2100626","url":null,"abstract":"<p><p>An endophytic <i>Pseudomonas fluorescens</i> (BsEB-1) was obtained from the roots of <i>Bletilla striata</i>. We investigated its growth-promoting properties and observed the impact of its inoculation on both the growth and polysaccharide content of <i>Bletilla striata</i> tubers. It was found that BsEB-1 possessed three growth-promoting activities: phosphate-solubilizing, produced indoleacetic acid (IAA) and siderophores, but had no nitrogen-fixing activity. BsEB-1 could rapidly attach to the root hairs of <i>Bletilla striata</i> tissue culture seedlings and endophytically colonize the region of maturation in the roots. It also significantly promoted the rooting and transplant survival rate of the seedlings, as well as the growth and expansion of the tubers, but did not increase their polysaccharide content. <i>Pseudomonas fluorescens</i> BsEB-1 exhibits potential for applications in the artificial planting of <i>Bletilla striata.</i></p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2100626"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40668129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The characteristic of Yatu morphogenesis and the efficacy of exogenous hormones on the development of Yatu during fruit development in 'Yali' pear (<i>Pyrus bretschneideri</i> Rehd.).","authors":"Ying Zhang,&nbsp;Wanjun Liu,&nbsp;Xiaoxin Shi,&nbsp;Yuxing Zhang,&nbsp;Guoqiang Du","doi":"10.1080/15592324.2022.2106075","DOIUrl":"https://doi.org/10.1080/15592324.2022.2106075","url":null,"abstract":"<p><p>Yatu is a protuberance formed on the base part of 'Yali' pear fruit, near the pedicel, causing a shape like a duck head termed Yatu. It is a typical phenotypic trait to evaluate fruit appearance quality. The mechanism for Yatu formation has not been clear yet. Here, 90.8% of fruits with Yatu generated in outer base part of fruits. Primitive cells of Yatu were found at 10 days after pollination (DAP). There were higher expression levels of <i>PbGA20ox2, PbIPT7a</i>, and <i>PbIPT5a</i>, lower transcription levels of <i>PbGA2ox1, PbNCED1</i>, and <i>PbNCED3</i> in outer base part of fruits at 10 DAP, accompanied with significantly higher levels of GA₃, ZR, (GA₃+ ZR)/ABA, and lower ABA content compared to that in the inner base part of fruits. GA₃ + 6-BA promoted Yatu development by increasing GA₃ content at 10 and 20 DAP, and ZR content at 20 DAP. PAC suppressed Yatu morphogenesis and development by increasing ABA level at 10 DAP. These results suggest that Yatu usually generated in outer base part of fruits, relatively higher GA₃ and ZR contents, lower ABA content promoted Yatu morphogenesis and development.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2106075"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40675275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microtubule cytoskeleton and mycorrhizal roots.","authors":"Tania Ho-Plágaro,&nbsp;María Isabel Tamayo-Navarrete,&nbsp;José M García Garrido","doi":"10.1080/15592324.2022.2031504","DOIUrl":"https://doi.org/10.1080/15592324.2022.2031504","url":null,"abstract":"<p><p>For the establishment of the Arbuscular Mycorrhiza (AM) symbiosis it is essential that epidermis and cortical cells from plant roots suffer a strong reorganization to allow the penetration of intracellular fungal hyphae. In the same manner, the new formation of a periarbuscular membrane and a symbiotic interface with specific compositions are required for a functional symbiosis. It is believed that the cytoskeleton of the plant host plays an essential role in these processes, particularly the microtubule (MT) cytoskeleton, as huge modifications have been observed in the MT array of root cells accompanying the establishment of the AM symbiosis. Recent research has established a link between microtubule rearrangements and arbuscule functioning. However, further research is required to elucidate the specific functions of MT cytoskeleton along the different stages of the arbuscule life cycle and to unravel the signals triggering these changes.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2031504"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10339287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel type of phytosulfokine, PSK-ε, positively regulates root elongation and formation of lateral roots and root nodules in <i>Medicago truncatula</i>.","authors":"Qi Di,&nbsp;Yi Li,&nbsp;Danping Zhang,&nbsp;Wei Wu,&nbsp;Lin Zhang,&nbsp;Xing Zhao,&nbsp;Li Luo,&nbsp;Liangliang Yu","doi":"10.1080/15592324.2022.2134672","DOIUrl":"https://doi.org/10.1080/15592324.2022.2134672","url":null,"abstract":"<p><p>Phytosulfokines (PSKs) are a class of tyrosine-sulfated pentapeptides. PSK-α, PSK-γ, and PSK-δ are three reported PSK members involved in regulating plant growth, development, and resistance to biotic and abiotic stresses. Here, we reported a novel type of PSK, PSK-ε with the sequence Y_SO3VY_SO3TN, and its precursor proteins (MtPSKε, LjPSKε, and GmPSKε), specifically from legume species. PSK-ε peptide differs from PSK-δ by one amino acid and is close to PSK-δ in the phylogenetic relationship. Expression profile analysis showed that <i>MtPSKε</i> was highly expressed in <i>Medicago truncatula</i> roots, especially in root tips and emerged lateral roots. Application of the synthetic sulfated PSK-ε peptide and overexpression of <i>MtPSKε</i> significantly promoted <i>M. truncatula</i> root elongation and increased lateral root number, probably by inducing cell division and expansion in roots. Furthermore, <i>MtPSKε</i> expression was induced by rhizobia infection and was detected in root nodules including nodule primordia. Both PSK-ε peptide treatment and <i>MtPSKε</i> overexpression significantly increased nodule number in <i>M. truncatula</i>. Taken together, these results demonstrate that PSK-ε, a novel type of phytosulfokine, positively regulates root elongation and formation of lateral root and root nodule in <i>M. truncatula</i>.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2134672"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9662189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10404800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The characterization and expression analysis under stress conditions of <i>PCST1</i> in <i>Arabidopsis</i>.","authors":"Hao Zhang,&nbsp;Ke Zhang,&nbsp;Tongtong Liu,&nbsp;Ying Zhang,&nbsp;Ziyan Tang,&nbsp;Jingao Dong,&nbsp;Fengru Wang","doi":"10.1080/15592324.2022.2134675","DOIUrl":"https://doi.org/10.1080/15592324.2022.2134675","url":null,"abstract":"<p><p>Analysis of <i>PCST1</i> expression characteristics and the role of <i>PCST1</i> in response to osmotic stress in <i>Arabidopsis thaliana</i>. The structure of <i>PCST1</i> was analyzed using Bioinformatics method. Real-time PCR, GUS tissue localization and subcellular localization were adopted to analyze the expression pattern of <i>PCST1</i> in Arabidopsis. To validate the transgenic positive strain of <i>PCST1</i> using Real-time PCR, overexpression experiments were performed in wild type. Full-length cDNA was cloned and connected into a binary vector with 35S promoter, and the construction was transformed into wild type. With NaCl and mannitol treatments, the germination rate, green leaves rate, physiological indexes were carried out and counted in Arabidopsis with overexpression of <i>PCST1</i> and T-DNA insertion mutants. The molecular mechanism of <i>PCST1</i> in response to osmotic stress in Arabidopsis was analyzed. Based on the bioinformatic analysis, PCST1 is a hydrophobin with 403 amino acids, and the molecular weight is 45.3236 KDa. It contains only the START (the lipid/sterol - binding StAR - related lipid transfer protein domains) conservative domain. PCST1 possesses phosphatidylcholine binding sites and transmembrane region. Expression pattern analysis showed that expression of <i>PCST1</i> increased with time. The <i>PCST1</i> widely expressed in Arabidopsis, including roots, axils of stem leaves, flowers (sepal, conductive tissue of the petal, thrum, anther and stigmas), and the top and basal parts of the siliquas. It mainly localized in cell membrane. The overexpression of <i>PCST1</i> enhanced the sensitivity to osmotic stress in <i>Arabidopsis</i> based on the germination rate. While expression of <i>PCST1</i> decreased, and the sensitivity to osmotic stress had no obvious change in Arabidopsis. Its molecular mechanism study showed, that PCST1 response to osmotic stress resistance by regulating the proline, betaine synthesis, as well as the expression of key genes <i>SOS, NCED, CIPK</i>. PCST1 is composed of 403 amino acids. The START conservative domain, a transmembrane structure, the phosphatidyl choline binding sites are contained in PCST1. It is localized in cytoplasmic membrane. The <i>PCST1</i> widely expressed in the root, leaf, flower and siliquas. NaCl and mannitol suppressed the expression of <i>PCST1</i> and PCST1 can negatively control action of <i>Arabidopsis</i> in the osmotic stress. PCST1 regulates the synthetic pathway of proline, betaine and the expression of <i>SOS, NCED</i> and <i>CIPK</i> in response to the osmotic stress resistance.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2134675"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9601564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10412333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arabidopsis NPF5.1 regulates ABA homeostasis and seed germination by mediating ABA uptake into the seed coat.","authors":"Takafumi Shimizu,&nbsp;Yuri Kanno,&nbsp;Shunsuke Watanabe,&nbsp;Mitsunori Seo","doi":"10.1080/15592324.2022.2095488","DOIUrl":"https://doi.org/10.1080/15592324.2022.2095488","url":null,"abstract":"<p><p>Abscisic acid (ABA) is a plant hormone that induces seed dormancy during seed development and inhibits seed germination after imbibition. Although ABA is synthesized in the seed coat (testa), endosperm, and embryo, the physiological roles of the hormone derived from each tissue are not fully understood. We found that the gene encoding an Arabidopsis ABA importer, <i>NPF5.1</i>, was expressed in the seed coat during seed development. Dry seeds of loss-of-function <i>npf5.1</i> mutants contained significantly higher levels of dihydrophaseic acid (DPA), an inactive ABA metabolite, than the wild type. The <i>npf5.1</i> mutant also had a slight increase in ABA content. An increase in DPA was prominent in the fraction containing the seed coat and endosperm. Seed germination of the <i>npf5.1</i> mutant was similar to the wild type in the presence of ABA or the gibberellin biosynthesis inhibitor paclobutrazol. However, a mutation in <i>NPF5.1</i> suppressed the paclobutrazol-resistant germination of <i>npf4.6</i>, a mutant impaired in an ABA importer expressed in the embryo. These results suggest that ABA uptake into the seed coat mediated by NPF5.1 is important for ABA homeostasis during seed development and for regulating seed germination.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2095488"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a0/7a/KPSB_17_2095488.PMC9298153.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40532940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of extremely low-frequency magnetic fields on light-induced electric reactions in wheat.","authors":"Marina Grinberg,&nbsp;Maxim Mudrilov,&nbsp;Elizaveta Kozlova,&nbsp;Vladimir Sukhov,&nbsp;Fedor Sarafanov,&nbsp;Andrey Evtushenko,&nbsp;Nikolay Ilin,&nbsp;Vladimir Vodeneev,&nbsp;Colin Price,&nbsp;Evgeny Mareev","doi":"10.1080/15592324.2021.2021664","DOIUrl":"https://doi.org/10.1080/15592324.2021.2021664","url":null,"abstract":"<p><p>Magnetic field oscillations resulting from atmospheric events could have an effect on growth and development of plants and on the responsive reactions of plants to other environmental factors. In the current work, extremely low-frequency magnetic field (14.3 Hz) was shown to modulate light-induced electric reactions of wheat (<i>Triticum aestivum</i> L.). Blue light-induced electric reaction in wheat leaf comprises depolarization and two waves of hyperpolarization resulting in an increase of the potential to a higher level compared to the dark one. Fluorescent and inhibitory analysis demonstrate a key role of calcium ions and calcium-dependent H⁺-ATPase of the plasma membrane in the development of the reaction. Activation of H⁺-ATPase by the increased calcium influx is suggested as a mechanism of the influence of magnetic field on light-induced electric reaction.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2021664"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39654122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}