Plant Signaling & Behavior最新文献

{"title":"<i>Pseudomonas fluorescens</i> BsEB-1: an endophytic bacterium isolated from the root of <i>Bletilla striata</i> that can promote its growth.","authors":"Yuanshuang Wu,&nbsp;Suhui Xiao,&nbsp;Jiaseng Qi,&nbsp;Yongchang Gong,&nbsp;Kunzhi Li","doi":"10.1080/15592324.2022.2100626","DOIUrl":"https://doi.org/10.1080/15592324.2022.2100626","url":null,"abstract":"<p><p>An endophytic <i>Pseudomonas fluorescens</i> (BsEB-1) was obtained from the roots of <i>Bletilla striata</i>. We investigated its growth-promoting properties and observed the impact of its inoculation on both the growth and polysaccharide content of <i>Bletilla striata</i> tubers. It was found that BsEB-1 possessed three growth-promoting activities: phosphate-solubilizing, produced indoleacetic acid (IAA) and siderophores, but had no nitrogen-fixing activity. BsEB-1 could rapidly attach to the root hairs of <i>Bletilla striata</i> tissue culture seedlings and endophytically colonize the region of maturation in the roots. It also significantly promoted the rooting and transplant survival rate of the seedlings, as well as the growth and expansion of the tubers, but did not increase their polysaccharide content. <i>Pseudomonas fluorescens</i> BsEB-1 exhibits potential for applications in the artificial planting of <i>Bletilla striata.</i></p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2100626"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40668129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The characteristic of Yatu morphogenesis and the efficacy of exogenous hormones on the development of Yatu during fruit development in 'Yali' pear (<i>Pyrus bretschneideri</i> Rehd.).","authors":"Ying Zhang,&nbsp;Wanjun Liu,&nbsp;Xiaoxin Shi,&nbsp;Yuxing Zhang,&nbsp;Guoqiang Du","doi":"10.1080/15592324.2022.2106075","DOIUrl":"https://doi.org/10.1080/15592324.2022.2106075","url":null,"abstract":"<p><p>Yatu is a protuberance formed on the base part of 'Yali' pear fruit, near the pedicel, causing a shape like a duck head termed Yatu. It is a typical phenotypic trait to evaluate fruit appearance quality. The mechanism for Yatu formation has not been clear yet. Here, 90.8% of fruits with Yatu generated in outer base part of fruits. Primitive cells of Yatu were found at 10 days after pollination (DAP). There were higher expression levels of <i>PbGA20ox2, PbIPT7a</i>, and <i>PbIPT5a</i>, lower transcription levels of <i>PbGA2ox1, PbNCED1</i>, and <i>PbNCED3</i> in outer base part of fruits at 10 DAP, accompanied with significantly higher levels of GA₃, ZR, (GA₃+ ZR)/ABA, and lower ABA content compared to that in the inner base part of fruits. GA₃ + 6-BA promoted Yatu development by increasing GA₃ content at 10 and 20 DAP, and ZR content at 20 DAP. PAC suppressed Yatu morphogenesis and development by increasing ABA level at 10 DAP. These results suggest that Yatu usually generated in outer base part of fruits, relatively higher GA₃ and ZR contents, lower ABA content promoted Yatu morphogenesis and development.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2106075"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40675275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microtubule cytoskeleton and mycorrhizal roots.","authors":"Tania Ho-Plágaro,&nbsp;María Isabel Tamayo-Navarrete,&nbsp;José M García Garrido","doi":"10.1080/15592324.2022.2031504","DOIUrl":"https://doi.org/10.1080/15592324.2022.2031504","url":null,"abstract":"<p><p>For the establishment of the Arbuscular Mycorrhiza (AM) symbiosis it is essential that epidermis and cortical cells from plant roots suffer a strong reorganization to allow the penetration of intracellular fungal hyphae. In the same manner, the new formation of a periarbuscular membrane and a symbiotic interface with specific compositions are required for a functional symbiosis. It is believed that the cytoskeleton of the plant host plays an essential role in these processes, particularly the microtubule (MT) cytoskeleton, as huge modifications have been observed in the MT array of root cells accompanying the establishment of the AM symbiosis. Recent research has established a link between microtubule rearrangements and arbuscule functioning. However, further research is required to elucidate the specific functions of MT cytoskeleton along the different stages of the arbuscule life cycle and to unravel the signals triggering these changes.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2031504"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10339287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel type of phytosulfokine, PSK-ε, positively regulates root elongation and formation of lateral roots and root nodules in <i>Medicago truncatula</i>.","authors":"Qi Di,&nbsp;Yi Li,&nbsp;Danping Zhang,&nbsp;Wei Wu,&nbsp;Lin Zhang,&nbsp;Xing Zhao,&nbsp;Li Luo,&nbsp;Liangliang Yu","doi":"10.1080/15592324.2022.2134672","DOIUrl":"https://doi.org/10.1080/15592324.2022.2134672","url":null,"abstract":"<p><p>Phytosulfokines (PSKs) are a class of tyrosine-sulfated pentapeptides. PSK-α, PSK-γ, and PSK-δ are three reported PSK members involved in regulating plant growth, development, and resistance to biotic and abiotic stresses. Here, we reported a novel type of PSK, PSK-ε with the sequence Y_SO3VY_SO3TN, and its precursor proteins (MtPSKε, LjPSKε, and GmPSKε), specifically from legume species. PSK-ε peptide differs from PSK-δ by one amino acid and is close to PSK-δ in the phylogenetic relationship. Expression profile analysis showed that <i>MtPSKε</i> was highly expressed in <i>Medicago truncatula</i> roots, especially in root tips and emerged lateral roots. Application of the synthetic sulfated PSK-ε peptide and overexpression of <i>MtPSKε</i> significantly promoted <i>M. truncatula</i> root elongation and increased lateral root number, probably by inducing cell division and expansion in roots. Furthermore, <i>MtPSKε</i> expression was induced by rhizobia infection and was detected in root nodules including nodule primordia. Both PSK-ε peptide treatment and <i>MtPSKε</i> overexpression significantly increased nodule number in <i>M. truncatula</i>. Taken together, these results demonstrate that PSK-ε, a novel type of phytosulfokine, positively regulates root elongation and formation of lateral root and root nodule in <i>M. truncatula</i>.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2134672"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9662189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10404800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The characterization and expression analysis under stress conditions of <i>PCST1</i> in <i>Arabidopsis</i>.","authors":"Hao Zhang,&nbsp;Ke Zhang,&nbsp;Tongtong Liu,&nbsp;Ying Zhang,&nbsp;Ziyan Tang,&nbsp;Jingao Dong,&nbsp;Fengru Wang","doi":"10.1080/15592324.2022.2134675","DOIUrl":"https://doi.org/10.1080/15592324.2022.2134675","url":null,"abstract":"<p><p>Analysis of <i>PCST1</i> expression characteristics and the role of <i>PCST1</i> in response to osmotic stress in <i>Arabidopsis thaliana</i>. The structure of <i>PCST1</i> was analyzed using Bioinformatics method. Real-time PCR, GUS tissue localization and subcellular localization were adopted to analyze the expression pattern of <i>PCST1</i> in Arabidopsis. To validate the transgenic positive strain of <i>PCST1</i> using Real-time PCR, overexpression experiments were performed in wild type. Full-length cDNA was cloned and connected into a binary vector with 35S promoter, and the construction was transformed into wild type. With NaCl and mannitol treatments, the germination rate, green leaves rate, physiological indexes were carried out and counted in Arabidopsis with overexpression of <i>PCST1</i> and T-DNA insertion mutants. The molecular mechanism of <i>PCST1</i> in response to osmotic stress in Arabidopsis was analyzed. Based on the bioinformatic analysis, PCST1 is a hydrophobin with 403 amino acids, and the molecular weight is 45.3236 KDa. It contains only the START (the lipid/sterol - binding StAR - related lipid transfer protein domains) conservative domain. PCST1 possesses phosphatidylcholine binding sites and transmembrane region. Expression pattern analysis showed that expression of <i>PCST1</i> increased with time. The <i>PCST1</i> widely expressed in Arabidopsis, including roots, axils of stem leaves, flowers (sepal, conductive tissue of the petal, thrum, anther and stigmas), and the top and basal parts of the siliquas. It mainly localized in cell membrane. The overexpression of <i>PCST1</i> enhanced the sensitivity to osmotic stress in <i>Arabidopsis</i> based on the germination rate. While expression of <i>PCST1</i> decreased, and the sensitivity to osmotic stress had no obvious change in Arabidopsis. Its molecular mechanism study showed, that PCST1 response to osmotic stress resistance by regulating the proline, betaine synthesis, as well as the expression of key genes <i>SOS, NCED, CIPK</i>. PCST1 is composed of 403 amino acids. The START conservative domain, a transmembrane structure, the phosphatidyl choline binding sites are contained in PCST1. It is localized in cytoplasmic membrane. The <i>PCST1</i> widely expressed in the root, leaf, flower and siliquas. NaCl and mannitol suppressed the expression of <i>PCST1</i> and PCST1 can negatively control action of <i>Arabidopsis</i> in the osmotic stress. PCST1 regulates the synthetic pathway of proline, betaine and the expression of <i>SOS, NCED</i> and <i>CIPK</i> in response to the osmotic stress resistance.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2134675"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9601564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10412333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arabidopsis NPF5.1 regulates ABA homeostasis and seed germination by mediating ABA uptake into the seed coat.","authors":"Takafumi Shimizu,&nbsp;Yuri Kanno,&nbsp;Shunsuke Watanabe,&nbsp;Mitsunori Seo","doi":"10.1080/15592324.2022.2095488","DOIUrl":"https://doi.org/10.1080/15592324.2022.2095488","url":null,"abstract":"<p><p>Abscisic acid (ABA) is a plant hormone that induces seed dormancy during seed development and inhibits seed germination after imbibition. Although ABA is synthesized in the seed coat (testa), endosperm, and embryo, the physiological roles of the hormone derived from each tissue are not fully understood. We found that the gene encoding an Arabidopsis ABA importer, <i>NPF5.1</i>, was expressed in the seed coat during seed development. Dry seeds of loss-of-function <i>npf5.1</i> mutants contained significantly higher levels of dihydrophaseic acid (DPA), an inactive ABA metabolite, than the wild type. The <i>npf5.1</i> mutant also had a slight increase in ABA content. An increase in DPA was prominent in the fraction containing the seed coat and endosperm. Seed germination of the <i>npf5.1</i> mutant was similar to the wild type in the presence of ABA or the gibberellin biosynthesis inhibitor paclobutrazol. However, a mutation in <i>NPF5.1</i> suppressed the paclobutrazol-resistant germination of <i>npf4.6</i>, a mutant impaired in an ABA importer expressed in the embryo. These results suggest that ABA uptake into the seed coat mediated by NPF5.1 is important for ABA homeostasis during seed development and for regulating seed germination.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2095488"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a0/7a/KPSB_17_2095488.PMC9298153.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40532940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of extremely low-frequency magnetic fields on light-induced electric reactions in wheat.","authors":"Marina Grinberg,&nbsp;Maxim Mudrilov,&nbsp;Elizaveta Kozlova,&nbsp;Vladimir Sukhov,&nbsp;Fedor Sarafanov,&nbsp;Andrey Evtushenko,&nbsp;Nikolay Ilin,&nbsp;Vladimir Vodeneev,&nbsp;Colin Price,&nbsp;Evgeny Mareev","doi":"10.1080/15592324.2021.2021664","DOIUrl":"https://doi.org/10.1080/15592324.2021.2021664","url":null,"abstract":"<p><p>Magnetic field oscillations resulting from atmospheric events could have an effect on growth and development of plants and on the responsive reactions of plants to other environmental factors. In the current work, extremely low-frequency magnetic field (14.3 Hz) was shown to modulate light-induced electric reactions of wheat (<i>Triticum aestivum</i> L.). Blue light-induced electric reaction in wheat leaf comprises depolarization and two waves of hyperpolarization resulting in an increase of the potential to a higher level compared to the dark one. Fluorescent and inhibitory analysis demonstrate a key role of calcium ions and calcium-dependent H⁺-ATPase of the plasma membrane in the development of the reaction. Activation of H⁺-ATPase by the increased calcium influx is suggested as a mechanism of the influence of magnetic field on light-induced electric reaction.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2021664"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39654122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and expression pattern analysis of the TCP transcription factor family in <i>Ginkgo biloba</i>.","authors":"Li Yu,&nbsp;Qiangwen Chen,&nbsp;Jiarui Zheng,&nbsp;Feng Xu,&nbsp;Jiabao Ye,&nbsp;Weiwei Zhang,&nbsp;Yongling Liao,&nbsp;Xiaoyan Yang","doi":"10.1080/15592324.2021.1994248","DOIUrl":"https://doi.org/10.1080/15592324.2021.1994248","url":null,"abstract":"<p><p>Plant-specific TCP transcription factors play an essential role in plant growth and development. They can regulate leaf curvature, flower symmetry and the synthesis of secondary metabolites. The flavonoids in <i>Ginkgo biloba</i> leaf are one of the main medicinally bioactivate compounds, which have pharmacological and beneficial health effects for humans. In this study, a total of 13 <i>TCP</i> genes were identified in <i>G. biloba</i>, and 5 of them belonged to PCF subclades (<i>GbTCP03, GbTCP07, GbTCP05, GbTCP13, GbTCP02</i>) while others belonged to CIN (<i>GbTCP01, GbTCP04, GbTCP06, GbTCP08, GbTCP09, GbTCP10, GbTCP11, GbTCP12</i>) subclades according to phylogenetic analysis. Numerous <i>cis</i>-acting elements related to various biotic and abiotic signals were predicted on the promoters by <i>cis</i>-element analysis, suggesting that the expression of <i>GbTCPs</i> might be co-regulated by multiple signals. Transcript abundance analysis exhibited that most of <i>GbTCPs</i> responded to multiple phytohormones. Among them, the relative expression levels of <i>GbTCP06, GbTCP11</i>, and <i>GbTCP13</i> were found to be significantly influenced by exogenous ABA, SA and MeJA application. In addition, a total of 126 miRNAs were predicted to target 9 <i>TCPs</i> (including <i>GbTCP01, GbTCP02, GbTCP04, GbTCP05, GbTCP06, GbTCP08, GbTCP11, GbTCP12, GbTCP13</i>). The correlation analysis between the expression level of <i>GbTCPs</i> and the flavonoid contents showed that <i>GbTCP03, GbTCP04, GbTCP07</i> might involve in flavonoid biosynthesis in <i>G. biloba</i>. In short, this study mainly provided a theoretical foundation for better understanding the potential function of <i>TCPs</i> in <i>G. biloba</i>.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"1994248"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3c/7f/KPSB_17_1994248.PMC9176236.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39728157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of <i>PsmiR319</i> during flower development in early- and late-flowering tree peonies cultivars.","authors":"Chenjie Zhang,&nbsp;Jiajia Shen,&nbsp;Can Wang,&nbsp;Zhanying Wang,&nbsp;Lili Guo,&nbsp;Xiaogai Hou","doi":"10.1080/15592324.2022.2120303","DOIUrl":"https://doi.org/10.1080/15592324.2022.2120303","url":null,"abstract":"<p><p>The flowering period is the most important ornamental trait of tree peony, while industrial development of tree peony has been limited by short flowering period. miR319 plays an important regulatory role in plant flowering. In the current study, the expression characteristics and evolution of <i>PsmiR319</i> in tree peony flowering was explored using 'Feng Dan' and 'Lian He', which are early-flowering and late-flowering varieties of tree peony, respectively. The structure, evolution, and target(s) of <i>PsmiR319</i> were analyzed by bioinformatics. Evolution analysis showed that pre-<i>PsmiR319</i> was distributed in 41 plant species, among which the length of the precursor sequence exhibited marked differences (between 52 and 308 bp). Pre-<i>PsmiR319</i> of tree peony was located close to the corresponding sequences of <i>Linum usitatissimum</i> and <i>Picea abies</i> in the phylogenetic tree, and in addition, could form a typical hairpin structure including a mature body with a length of 20 bp located on the 3p arm and part of the loop sequence. The mature sequence of miR319 was highly conserved among different species. Target genes of <i>PsmiR319</i> include MYB-related transcription factor in tree peony. Expression of <i>PsmiR319</i>, assayed by qRT-PCR, differed between 'Feng Dan' and 'Lian He' during different flower development periods. <i>PsmiR319</i> and its target gene showed a negative expression regulation relationship during the periods of CE (color exposure), BS (blooming stage), IF (initial flowering), and HO (half opening) in the early-flowering 'Feng Dan', and the same in FB (Full blooming) periods of late-flowering 'Lian He'. Findings from this study provide a reference for further investigation into the mechanism of miR319 in the development of different varieties of tree peony.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2120303"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9542857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33491313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison analysis of metabolite profiling in seeds and bark of <i>Ulmus parvifolia</i>, a Chinese medicine species.","authors":"MingLong Yin,&nbsp;ChuanRong Li,&nbsp;YuShan Wang,&nbsp;JunHui Fu,&nbsp;YangYang Sun,&nbsp;Qian Zhang","doi":"10.1080/15592324.2022.2138041","DOIUrl":"https://doi.org/10.1080/15592324.2022.2138041","url":null,"abstract":"<p><p><i>Ulmus parvifolia</i> (<i>U. parvifolia</i>) is a Chinese medicine plant whose bark and leaves are used in the treatment of some diseases such as inflammation, diarrhea and fever. However, metabolic signatures of seeds have not been studied. The seeds and bark of <i>U. parvifolia</i> collected at the seed ripening stage were used for metabolite profiling analysis through the untargeted metabolomics approach. A total of 2,578 and 2,207 metabolites, while 503 and 132 unique metabolites were identified in seeds and bark, respectively. Additionally, 574 differential metabolites (DEMs) were detected in the two different organs of <i>U. parvifolia</i>, which were grouped into 52 classes. Most kinds of metabolites classed into prenol lipids class. The relative content of flavonoids class was the highest. DEMs contained some bioactive compounds (e.g., flavonoids, terpene glycosides, triterpenoids, sesquiterpenoids) with antioxidant, anti-inflammatory, and anti-cancer activities. Most kinds of flavonoids and sesquiterpenes were up-regulated in seeds. There were more varieties of terpene glycosides and triterpenoids showing up-regulated in bark. The pathway enrichment was performed, while flavonoid biosynthesis, flavone and flavonol biosynthesis were worthy of attention. This study identified DEMs with pharmaceutical value between seeds and bark during seed maturation and offered a molecular basis for alternative or complementary use of seeds and bark of <i>U. parvifolia</i> as a Chinese medicinal material.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2138041"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10412773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}