sspp3 /CBL7负调控拟南芥质膜H+- atp酶，调控下胚轴伸长。

IF 2.8 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Signaling & Behavior Pub Date : 2022-12-31 DOI:10.1080/15592324.2022.2092699

Xiao Liu, Yujiao Wu, Haiqi Fu, Shu Song, Qian He, Yongqing Yang

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引用次数: 1

摘要

下胚轴伸长的调控是植物生长发育的一个重要过程，它取决于质膜H+- atp酶的活性。在本研究中，我们发现拟南芥蛋白SOS3-LIKE钙结合蛋白3 (sspp3)负调控酵母中PM H+- atp酶活性和暗生幼苗下胚轴伸长。酵母双杂交实验表明，sspp3与拟南芥PM H+- atp酶家族的代表性成员相互作用。在RS-72酵母中进行的实验表明，sspp3负调控PM H+- atp酶活性依赖性酵母细胞的生长。在功能缺失突变体scabp3中，下胚轴伸长得到促进，而在过表达系中，下胚轴伸长受到抑制。我们认为sspp3通过负调控PM H+- atp酶活性来调节下胚轴伸长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

SCaBP3/CBL7 negatively regulates the plasma membrane H+-ATPase and modulates hypocotyl elongation in Arabidopsis.

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SCaBP3/CBL7 negatively regulates the plasma membrane H⁺-ATPase and modulates hypocotyl elongation in Arabidopsis.

The regulation of hypocotyl elongation is an important process in plant growth and development and depends on the activity of the plasma membrane (PM) H⁺-ATPase. In this study, we found that the Arabidopsis protein SOS3-LIKE CALCIUM BINDING PROTEIN3 (SCaBP3) negatively regulates PM H⁺-ATPase activity in yeast and hypocotyl elongation in dark-grown seedlings. Yeast two-hybrid assays showed that SCaBP3 interacts with representative members of the Arabidopsis PM H⁺-ATPase family. Experiments in RS-72 yeast showed that SCaBP3 negatively regulates PM H⁺-ATPase activity-dependent yeast cell growth. Hypocotyl elongation was promoted in the loss-of-function mutant scabp3 and inhibited in SCaBP3 overexpression lines of Arabidopsis. We propose that SCaBP3 modulates hypocotyl elongation by negatively regulating PM H⁺-ATPase activity.

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来源期刊

Plant Signaling & Behavior Agricultural and Biological Sciences-Plant Science

CiteScore

6.00

自引率

3.40%

发文量

111

期刊介绍： Plant Signaling & Behavior, a multidisciplinary peer-reviewed journal published monthly online, publishes original research articles and reviews covering the latest aspects of signal perception and transduction, integrative plant physiology, and information acquisition and processing.