A novel type of phytosulfokine, PSK-ε, positively regulates root elongation and formation of lateral roots and root nodules in Medicago truncatula.

IF 2.8 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Qi Di, Yi Li, Danping Zhang, Wei Wu, Lin Zhang, Xing Zhao, Li Luo, Liangliang Yu
{"title":"A novel type of phytosulfokine, PSK-ε, positively regulates root elongation and formation of lateral roots and root nodules in <i>Medicago truncatula</i>.","authors":"Qi Di,&nbsp;Yi Li,&nbsp;Danping Zhang,&nbsp;Wei Wu,&nbsp;Lin Zhang,&nbsp;Xing Zhao,&nbsp;Li Luo,&nbsp;Liangliang Yu","doi":"10.1080/15592324.2022.2134672","DOIUrl":null,"url":null,"abstract":"<p><p>Phytosulfokines (PSKs) are a class of tyrosine-sulfated pentapeptides. PSK-α, PSK-γ, and PSK-δ are three reported PSK members involved in regulating plant growth, development, and resistance to biotic and abiotic stresses. Here, we reported a novel type of PSK, PSK-ε with the sequence Y<sub>SO3</sub>VY<sub>SO3</sub>TN, and its precursor proteins (MtPSKε, LjPSKε, and GmPSKε), specifically from legume species. PSK-ε peptide differs from PSK-δ by one amino acid and is close to PSK-δ in the phylogenetic relationship. Expression profile analysis showed that <i>MtPSKε</i> was highly expressed in <i>Medicago truncatula</i> roots, especially in root tips and emerged lateral roots. Application of the synthetic sulfated PSK-ε peptide and overexpression of <i>MtPSKε</i> significantly promoted <i>M. truncatula</i> root elongation and increased lateral root number, probably by inducing cell division and expansion in roots. Furthermore, <i>MtPSKε</i> expression was induced by rhizobia infection and was detected in root nodules including nodule primordia. Both PSK-ε peptide treatment and <i>MtPSKε</i> overexpression significantly increased nodule number in <i>M. truncatula</i>. Taken together, these results demonstrate that PSK-ε, a novel type of phytosulfokine, positively regulates root elongation and formation of lateral root and root nodule in <i>M. truncatula</i>.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2134672"},"PeriodicalIF":2.8000,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9662189/pdf/","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Signaling & Behavior","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/15592324.2022.2134672","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 2

Abstract

Phytosulfokines (PSKs) are a class of tyrosine-sulfated pentapeptides. PSK-α, PSK-γ, and PSK-δ are three reported PSK members involved in regulating plant growth, development, and resistance to biotic and abiotic stresses. Here, we reported a novel type of PSK, PSK-ε with the sequence YSO3VYSO3TN, and its precursor proteins (MtPSKε, LjPSKε, and GmPSKε), specifically from legume species. PSK-ε peptide differs from PSK-δ by one amino acid and is close to PSK-δ in the phylogenetic relationship. Expression profile analysis showed that MtPSKε was highly expressed in Medicago truncatula roots, especially in root tips and emerged lateral roots. Application of the synthetic sulfated PSK-ε peptide and overexpression of MtPSKε significantly promoted M. truncatula root elongation and increased lateral root number, probably by inducing cell division and expansion in roots. Furthermore, MtPSKε expression was induced by rhizobia infection and was detected in root nodules including nodule primordia. Both PSK-ε peptide treatment and MtPSKε overexpression significantly increased nodule number in M. truncatula. Taken together, these results demonstrate that PSK-ε, a novel type of phytosulfokine, positively regulates root elongation and formation of lateral root and root nodule in M. truncatula.

Abstract Image

Abstract Image

Abstract Image

一种新型植物磺胺酮PSK-ε对苜蓿根伸长、侧根和根瘤的形成具有正向调控作用。
植物硫代因子(Phytosulfokines, pks)是一类酪氨酸磺酸五肽。PSK-α、PSK-γ和PSK-δ是三个已报道的PSK成员,参与调节植物的生长、发育以及对生物和非生物胁迫的抗性。在这里,我们报道了一种新的PSK类型,序列为YSO3VYSO3TN的PSK-ε及其前体蛋白(MtPSKε, LjPSKε和GmPSKε),特别是来自豆类物种。PSK-ε肽与PSK-δ相差1个氨基酸,在系统发育关系上与PSK-δ接近。表达谱分析表明,MtPSKε在苜蓿根中高度表达,尤其是根尖和侧根。应用人工合成的硫酸化PSK-ε肽和过表达MtPSKε显著促进了白曲根的伸长和侧根数量的增加,这可能是通过诱导根内细胞的分裂和扩增来实现的。此外,根瘤菌感染诱导MtPSKε表达,并在根瘤包括根瘤原基中检测到。PSK-ε肽处理和MtPSKε过表达均显著增加了截骨松瘤的数量。综上所述,PSK-ε是一种新型植物磺酰素,对根伸长、侧根和根瘤的形成具有正向调控作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Plant Signaling & Behavior
Plant Signaling & Behavior Agricultural and Biological Sciences-Plant Science
CiteScore
6.00
自引率
3.40%
发文量
111
期刊介绍: Plant Signaling & Behavior, a multidisciplinary peer-reviewed journal published monthly online, publishes original research articles and reviews covering the latest aspects of signal perception and transduction, integrative plant physiology, and information acquisition and processing.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信