Plant Signaling & Behavior最新文献

{"title":"Differential regulation of jasmonate responses in multiple <i>jaz</i> mutants.","authors":"Yue Zhang,&nbsp;Bei Liu,&nbsp;Jiaqi Zhai,&nbsp;Qinglei Wang,&nbsp;Susheng Song","doi":"10.1080/15592324.2021.1997240","DOIUrl":"https://doi.org/10.1080/15592324.2021.1997240","url":null,"abstract":"<p><p>The phytohormones jasmonates (JAs) regulate diverse aspects of plant growth and defense responses. The JA-ZIM domain (JAZ) family of repressors are targeted by the JA receptor Coronatine Insensitive 1 for ubiquitination and subsequent degradation via the 26S proteasome. We previously investigated the functions of JAZs in JA responses by analyzing <i>jaz</i> mutants of the phylogenetic group I (<i>jaz1/2/5/6</i>), group II/III (<i>jaz10/11/12</i>), group IV/V (<i>jaz3/4/7/9</i> and <i>jaz3/4/7/8/9</i>), and their high-order mutant <i>jaz1/2/3/4/5/6/7/9/10/11/12</i>. Here, we examined JA-regulated root growth, apical hook curvature, flowering time, and defense against the insect <i>Spodoptera exigua</i> in the intermediate <i>jaz</i> mutants <i>jaz1/2/5/6/10/11/12, jaz1/2/3/4/5/6/7/9</i>, and <i>jaz3/4/7/8/9/10/11/12</i>. This study shows that these <i>jaz</i> mutants differentially affect JA responses, suggesting the complexity of JA pathway in these multiple <i>jaz</i> mutants.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"1997240"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8903784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39578373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ROS and calcium oscillations are required for polarized root hair growth.","authors":"Xinxin Zhang,&nbsp;Ang Bian,&nbsp;Teng Li,&nbsp;Lifei Ren,&nbsp;Li Li,&nbsp;Yuan Su,&nbsp;Qun Zhang","doi":"10.1080/15592324.2022.2106410","DOIUrl":"https://doi.org/10.1080/15592324.2022.2106410","url":null,"abstract":"<p><p>Root hairs are filamentous extensions from epidermis of plant roots with growth limited to the apical dome. Cell expansion undergoes tightly regulated processes, including the coordination between cell wall loosening and cell wall crosslinking, to form the final shape and size. Tip-focused gradients and oscillations of reactive oxygen species (ROS) together with calcium ions (Ca²⁺) as indispensable regulated mechanisms control rapid and polarized elongation of root hair cells. ROS homeostasis mediated by plasma membrane-localized NADPH oxidases, known as respiratory burst oxidase homologues (RBOHs), and class III cell wall peroxidases (PRXs), modulates cell wall properties during cell expansion. The expression levels of <i>RBOHC</i>, an NADPH oxidase that produces ROS, and class III <i>PRXs</i> are directly upregulated by <i>ROOT HAIR DEFECTIVE SIX-LIKE 4</i> (<i>RSL4</i>), encoding a basic-helix-loop-helix (bHLH) transcription factor, to modulate root hair elongation. Cyclic nucleotide-gated channels (CNGCs), as central regulators of Ca²⁺ oscillations, also regulate root hair extension. Here, we review how the gradients and oscillations of Ca²⁺ and ROS interact to promote the expansion of root hair cells.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2106410"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359386/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40689640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternative or cytochrome? Respiratory pathways in traps of aquatic carnivorous bladderwort <i>Utricularia reflexa</i>.","authors":"Andrej Pavlovič,&nbsp;Jana Jakšová,&nbsp;Martin Hrivňacký,&nbsp;Lubomír Adamec","doi":"10.1080/15592324.2022.2134967","DOIUrl":"https://doi.org/10.1080/15592324.2022.2134967","url":null,"abstract":"<p><p>Carnivorous plants of the genus <i>Utricularia</i> (bladderwort) form modified leaves into suction bladder traps. The bladders are metabolically active plant tissue with high rates of mitochondrial respiration (R_D). In general, plants possess two mitochondrial electron transport pathways to reduce oxygen to water: cytochrome and an alternative. Due to the high metabolic rate in the bladders, it is tempting to assume that the bladders prefer the cytochrome <i>c</i> oxidative pathway. Surprisingly, we revealed that alternative oxidase (AOX), which yields only a little ATP, is much more abundant in the bladders of <i>Utricularia reflexa</i> in comparison with the shoots. This pattern is similar to the carnivorous plants with passive pitcher traps (e.g. <i>Sarracenia, Nepenthes</i>) and seems to be widespread across many carnivorous taxa. The exact role of AOX in the traps of carnivorous plants remains to be investigated.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2134967"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590445/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10760645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosynthetic electron transport rate and root dynamics of finger millet in response to <i>Trichoderma harzianum</i>.","authors":"Ramwant Gupta,&nbsp;Munna Singh,&nbsp;Bibi Rafeiza Khan","doi":"10.1080/15592324.2022.2146373","DOIUrl":"https://doi.org/10.1080/15592324.2022.2146373","url":null,"abstract":"<p><p>Finger millet (ragi) is the main food grain for many people, especially in the arid and semiarid regions of developing countries in Asia and Africa. The grains contain an exceptionally higher amount of Ca (>300 mg/100 g) when compared to other major cereals. For sustainable production of ragi in the current scenario of climate change, this study aimed to evaluate the impact of <i>Trichoderma harzianum</i> (TRI) on ragi performance. The performance of photosynthetic pigment pool, photosynthetic apparatus, and root dynamics of three varieties of ragi (PRM-1, PRM-701, and PRM-801) in response to four treatments <i>viz</i>., C (soil), S+ TRI (soil + <i>Trichoderma</i>), farmyard manure (soil+ FYM), and FYM+TRI (Soil + FYM + <i>Trichoderma</i>) were studied. Results have shown a significant increase in the photosynthetic pigment pool and optimized functional and structural integrity of the photosynthetic apparatus in response to the combination of farmyard manure (FYM) with TRI. Higher yield parameters <i>viz</i>., φ(Po) and φ(Eo), δ(Ro), efficiency ψ(Eo), performance indices - PI_abs and PI_total, and enhanced root canopy and biomass were observed in all three varieties. Improved electron transport from PSII to PSI, root canopy and biomass, may also suitably favor biological carbon sequestration to retain soil health and plant productivity in case grown in association with FYM and TRI.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2146373"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10426692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Better together: the use of virus-induced gene silencing technique to repress the expression of two endogenous citrus genes simultaneously.","authors":"Nabil Killiny","doi":"10.1080/15592324.2022.2106079","DOIUrl":"https://doi.org/10.1080/15592324.2022.2106079","url":null,"abstract":"<p><p>Virus-induced gene silencing is a promising technique for functional genomics studies. <i>Citrus tristeza virus</i> was employed successfully to create an infectious clone that was used to silence endogenous citrus genes. Phytoene desaturase (<i>PDS</i>) and delta (δ)-aminolevulinic acid dehydratase (<i>ALAD</i>) were targeted successfully in citrus. Silencing <i>PDS</i> usually results in a photo-bleached leaf phenotype while silencing <i>ALAD</i> causes discrete yellow spots in leaves. Silencing two or more genes simultaneously using the same infectious clone could be difficult due to the capacity of the plasmid and subsequent cloning. On the other hand, inoculating a new construct into a citrus plant pre-infected with another construct fails due to the superinfection exclusion phenomenon. Herein, I report our successful trials whereby we simultaneously graft-inoculate constructs targeting <i>PDS</i> and <i>ALAD</i>. The budwoods were graft-inoculated into the same tree but on two different branches. Interestingly, a new phenotype was produced because of the silencing of the two genes, which we called \"color-breaking\". The phenotype was observed in both branches. Gene expression analysis showed a significant reduction of <i>PDS</i> and <i>ALAD</i> transcripts. This finding suggests the possibility of targeting more than one gene using different constructs, however, the graft-inoculation must be at the same time.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2106079"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40664623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A plant cell wall-associated kinase encoding gene is dramatically downregulated during nematode infection of potato.","authors":"Shiyan Chen,&nbsp;Lili Cui,&nbsp;Xiaohong Wang","doi":"10.1080/15592324.2021.2004026","DOIUrl":"https://doi.org/10.1080/15592324.2021.2004026","url":null,"abstract":"<p><p>Plant cell wall associated kinases (WAKs) and WAK-like kinases (WAKLs) have been increasingly recognized as important regulators of plant immunity against various plant pathogens. However, the role of the <i>WAK</i>/<i>WAKL</i> family in plant-nematode interactions remains to be determined. Here, we analyzed a <i>WAK</i>-encoding gene (<i>Soltu.DM.02G029720.1</i>) from potato (<i>Solanum tuberosum</i>). The <i>Soltu.DM.02G029720.1</i> encoded protein contains domains characteristic of WAK/WAKL proteins and shows the highest similarity to SlWAKL2 from tomato (<i>S. lycopersicum</i>). We thus named the gene as <i>StWAKL2</i>. Phylogenetic analysis of a wide range of plant WAKs/WAKLs further revealed close similarity of StWAKL2 to three WAK/WAKL proteins demonstrated to play a role in disease resistance. To gain insights into the potential regulation and function of <i>StWAKL2</i>, transgenic potato lines containing the <i>StWAKL2</i> promoter fused to the β-glucuronidase (GUS) reporter gene were generated and used to investigate <i>StWAKL2</i> expression during plant development and upon nematode infection. Histochemical analyses revealed that <i>StWAKL2</i> has specific expression patterns in potato leaf and root tissues. During nematode infection, GUS activity was mostly undetected at nematode infection sites over the course of nematode parasitism, although strong GUS activity was observed in root tissues adjacent to the infection region. Furthermore, mining of the transcriptomic data derived from cyst nematode infection of Arabidopsis roots identified a few <i>WAK</i>/<i>WAKL</i> genes, including a <i>StWAKL2</i> homologue, found to be significantly down-regulated in nematode-induced feeding sites. These results indicated that specific suppression of <i>WAK</i>/<i>WAKL</i> genes in nematode-induced feeding sites might be crucial for cyst nematodes to achieve successful infection of host plants. Further studies are needed to uncover the role of <i>WAK</i>/<i>WAKL</i> genes in plant defenses against nematode infection.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2004026"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39770900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and functional diversification of dihydroflavonol 4-Reductase gene <i>HvDFR</i> from <i>Hosta ventricosa</i> indicate its role in driving anthocyanin accumulation.","authors":"Shijie Qin,&nbsp;Yitong Liu,&nbsp;Baiqi Cui,&nbsp;Jianlin Cheng,&nbsp;Shuying Liu,&nbsp;Hongzhang Liu","doi":"10.1080/15592324.2021.2010389","DOIUrl":"https://doi.org/10.1080/15592324.2021.2010389","url":null,"abstract":"<p><p>Anthocyanins are natural colorants are synthesized in a branch of the flavonoid pathway. Dihydroflavonol-4reductase (DFR) catalyzes dihydroflavonoids into anthocyanins biosynthesis, which is a key regulatory enzyme of anthocyanin biosynthesis in plants. <i>Hosta ventricosa</i> is an ornamental plant with elegant flowers and rich colorful leaves. How the function of HvDFR contributes to the anthocyanins biosynthesis is still unknown. In this study, the DFR homolog was identified from <i>H. ventricosa</i> and sequence analysis showed that HvDFR possessed the conserved NADPH binding and catalytic domains. A phylogenetic analysis showed that HvDFR was close to the clade formed with MaDFR and HoDFR in Asparagaceae. Gene expression analysis revealed that HvDFR was constitutive expressed in all tissues and expressed highly in flower as well as was positively correlated with anthocyanin content. In addition, the subcellular location of HvDFR showed that is in the nucleus and cell membrane. Overexpression of HvDFR in transgenic tobacco lines enhanced the anthocyanins accumulation along with the key genes upregulated, such as F3H, F3'H, ANS, and UFGT. Our results indicated a functional activity of the HvDFR, which provide an insight into the regulation of anthocyanins content in <i>H. ventricosa.</i></p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2010389"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39872688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A metal tolerance protein, MTP10, is required for the calcium and magnesium homeostasis in <i>Arabidopsis</i>.","authors":"Haiman Ge,&nbsp;Qiaolin Shao,&nbsp;Jinlin Chen,&nbsp;Jiahong Chen,&nbsp;Xueqin Li,&nbsp;Yu Tan,&nbsp;Wenzhi Lan,&nbsp;Lei Yang,&nbsp;Yuan Wang","doi":"10.1080/15592324.2021.2025322","DOIUrl":"https://doi.org/10.1080/15592324.2021.2025322","url":null,"abstract":"<p><p>Nutrient antagonism typically refers to the fact that too high a concentration of one nutrient inhibits the absorption of another nutrient. In plants, Ca²⁺ (Calcium) and Mg²⁺ (Magnesium) are the two most abundant divalent cations, which are known to have antagonistic interactions. Hence, maintaining their homeostasis is crucial for plant growth and development. In this study, we showed that MTP10 (Metal Tolerance Protein 10) is an important regulator for maintaining homeostasis of Mg and Ca in Arabidopsis. The mtp10 mutant displayed severe growth retardation in the presence of excess Mg²⁺, to which the addition of Ca²⁺ was able to rescue the phenotype of mtp10 mutant. Additionally, the deficiency of Ca²⁺ in the culture medium accelerated the high-Mg sensitivity of the mtp10 mutant. The yeast complementation assay suggested that AtMTP10 had no Ca²⁺ transport activity. And the ICP-MS data further confirmed the antagonistic relationship between Ca²⁺ and Mg²⁺, with the addition of Ca²⁺ reducing the excessive accumulation of Mg²⁺ and high-Mg inhibiting the uptake of Ca²⁺. We conclude that the Arabidopsis MTP10 is essential for the regulation of Mg and Ca homeostasis.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2025322"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39917094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid transporter LSR1 positively regulates leaf senescence in Arabidopsis.","authors":"Guanping Feng,&nbsp;Yihui Zhong,&nbsp;Wenying Zou","doi":"10.1080/15592324.2021.2007328","DOIUrl":"https://doi.org/10.1080/15592324.2021.2007328","url":null,"abstract":"<p><p>Senescence is the final stage in the life history of a leaf, whereby plants relocate nutrients from leaves to other developing organs. Recent efforts have begun to focus on understanding the network-based molecular mechanism that incorporates various environmental signals and leaf age information and involves a complex process with the coordinated actions of multiple pathways. Here, we identified a novel participant, named LSR1 (Leaf Senescence Related 1), that involved in the regulation of leaf senescence. Loss-of-function <i>lsr1-1</i> mutant showed delayed leaf senescence whereas the overexpression of <i>LSR1</i> accelerated senescence. LSR1 encodes a lipid transfer protein, and the results show that the protein is located in chloroplast and intercellular space. The LSR1 may be involved in the regulation of leaf senescence by transporting lipids in plants.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":" ","pages":"2007328"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39756868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silencing of <i>SlDRB1</i> gene reduces resistance to tomato yellow leaf curl virus (TYLCV) in tomato (<i>Solanum lycopersicum</i>).","authors":"Xin Huang,&nbsp;Jianming Wei,&nbsp;Dan Wu,&nbsp;Na Mi,&nbsp;Sili Fang,&nbsp;Yao Xiao,&nbsp;Yunzhou Li","doi":"10.1080/15592324.2022.2149942","DOIUrl":"https://doi.org/10.1080/15592324.2022.2149942","url":null,"abstract":"<p><p>Double-stranded RNA-binding proteins are small molecules in the RNA interference (RNAi) pathway that form the RNAi machinery together with the Dicer-like protein (DCL) as a cofactor. This machinery cuts double-stranded RNA (dsRNA) to form multiple small interfering RNAs (siRNAs). Our goal was to clarify the function of <i>DRB</i> in tomato resistant to TYLCV. In this experiment, the expression of the <i>SlDRB1</i> and <i>SlDRB4</i> genes was analyzed in tomato leaves by qPCR, and the function of <i>SlDRB1</i> and <i>SlDRB4</i> in resistance to TYLCV was investigated by virus-induced gene silencing (VIGS). Then, peroxidase activity was determined. The results showed that the expression of <i>SlDRB1</i> gradually increased after inoculation of 'dwarf tomato' plants with tomato yellow leaf curl virus (TYLCV), but this gene was suppressed after 28 days. Resistance to TYLCV was significantly weakened after silencing of the <i>SlDRB1</i> gene. However, there were no significant expression differences in <i>SlDRB4</i> after TYLCV inoculation. Our study showed that silencing <i>SlDRB1</i> attenuated the ability of tomato plants to resist virus infection; therefore, <i>SlDRB1</i> may play a key role in the defense against TYLCV in tomato plants, whereas <i>SlDRB4</i> is likely not involved in this defense response. Taken together, These results suggest that the <i>DRB</i> gene is involved in the mechanism of antiviral activity.</p>","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":"17 1","pages":"2149942"},"PeriodicalIF":2.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10422684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}