兰花品种石斛DnMSI1基因的克隆及表达分析。

IF 2.8 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Signaling & Behavior Pub Date : 2022-12-31 Epub Date: 2022-01-10 DOI:10.1080/15592324.2021.2021649

Baolu Cui, Min Huang, Chongdai Guo, Ruihong Li, Yuqi Wang

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引用次数: 3

摘要

WD40重复蛋白是酵母MSI1的同源物，在植物中是保守的，参与蛋白复合物，在植物发育中起基础作用。虽然一些msi1样蛋白已经在植物中被克隆和鉴定，但msi1样蛋白在二年生观赏植物石斛中的作用尚不清楚。本文报道了利用RACE技术克隆石斛(Dendrobium nobile Lindl)的DnMSI1基因。我们发现DnMSI1的表达受GA3和TDZ的诱导，但受ABA、PP333和干旱和盐胁迫的抑制。此外，DnMSI1在拟南芥中的过表达导致其对NaCl胁迫的耐受性降低。上述结果表明，DnMSI1在调节石斛耐盐性中起负调控作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Cloning and expression analysis of DnMSI1 gene in orchid species Dendrobium nobile Lindl.

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Cloning and expression analysis of DnMSI1 gene in orchid species Dendrobium nobile Lindl.

WD40 repeat proteins, the homologs of yeast MSI1, are conserved in plants, participating in protein complexes and playing fundamental functions in plant development. Although several MSI1-like proteins have been cloned and characterized in plants, the roles of MSI1-like proteins in the biennial ornamental plant, Dendrobium nobile Lindl, are still unclear. Here, we report the cloning of the DnMSI1 gene from Dendrobium nobile Lindl with RACE technology. We found that DnMSI1 expression was induced by GA₃ and TDZ but inhibited by ABA, PP333, and drought and salt stress. Furthermore, DnMSI1 over-expression in Arabidopsis resulted in decreased tolerance to NaCl stress. These results suggest that DnMSI1 plays negative regulation roles in regulating salinity-stress resistance in Dendrobium nobile Lindl.

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来源期刊

Plant Signaling & Behavior Agricultural and Biological Sciences-Plant Science

CiteScore

6.00

自引率

3.40%

发文量

111

期刊介绍： Plant Signaling & Behavior, a multidisciplinary peer-reviewed journal published monthly online, publishes original research articles and reviews covering the latest aspects of signal perception and transduction, integrative plant physiology, and information acquisition and processing.