Plant Direct最新文献

{"title":"Cultivated sunflower (Helianthus annuus L.) has lower tolerance of moderate drought stress than its con‐specific wild relative, but the underlying traits remain elusive","authors":"Vivian H. Tran, Kristen M. Nolting, Lisa A. Donovan, Andries A. Temme","doi":"10.1002/pld3.581","DOIUrl":"https://doi.org/10.1002/pld3.581","url":null,"abstract":"Cultivated crops are generally expected to have less abiotic stress tolerance than their wild relatives. However, this assumption is not well supported by empirical literature and may depend on the type of stress and how it is imposed, as well as the measure of tolerance being used. Here, we investigated whether wild and cultivated accessions of <jats:styled-content style=\"fixed-case\"><jats:italic>Helianthus annuus</jats:italic></jats:styled-content> differed in stress tolerance assessed as proportional decline in biomass due to drought and whether wild and cultivated accessions differed in trait responses to drought and trait associations with tolerance. In a greenhouse study, <jats:styled-content style=\"fixed-case\"><jats:italic>H. annuus</jats:italic></jats:styled-content> accessions in the two domestication classes (eight cultivated and eight wild accessions) received two treatments: a well‐watered control and a moderate drought implemented as a dry down followed by maintenance at a predetermined soil moisture level with automated irrigation. Treatments were imposed at the seedling stage, and plants were harvested after 2 weeks of treatment. The proportional biomass decline in response to drought was 24% for cultivated <jats:styled-content style=\"fixed-case\"><jats:italic>H. annuus</jats:italic></jats:styled-content> accessions but was not significant for the wild accessions. Thus, using the metric of proportional biomass decline, the cultivated accessions had less drought tolerance. Among accessions, there was no tradeoff between drought tolerance and vigor assessed as biomass in the control treatment. In a multivariate analysis, wild and cultivated accessions did not differ from each other or in response to drought for a subset of morphological, physiological, and allocational traits. Analyzed individually, traits varied in response to drought in wild and/or cultivated accessions, including declines in specific leaf area, leaf theoretical maximum stomatal conductance (g<jats:sub>smax</jats:sub>), and stomatal pore length, but there was no treatment response for stomatal density, succulence, or the ability to osmotically adjust. Focusing on traits associations with tolerance, plasticity in g<jats:sub>smax</jats:sub> was the most interesting because its association with tolerance differed by domestication class (although the effects were relatively weak) and thus might contribute to lower tolerance of cultivated sunflower. Our <jats:styled-content style=\"fixed-case\"><jats:italic>H. annuus</jats:italic></jats:styled-content> results support the expectation that stress tolerance is lower in crops than wild relatives under some conditions. However, determining the key traits that underpin differences in moderate drought tolerance between wild and cultivated <jats:styled-content style=\"fixed-case\"><jats:italic>H. annuus</jats:italic></jats:styled-content> remains elusive.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"1 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140565316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A K homology (KH) domain protein identified by a forward genetic screen affects bundle sheath anatomy in Arabidopsis thaliana","authors":"Zahida Bano, Peter Westhoff","doi":"10.1002/pld3.577","DOIUrl":"https://doi.org/10.1002/pld3.577","url":null,"abstract":"Because of their photosynthetic capacity, leaves function as solar panels providing the basis for the growth of the entire plant. Although the molecular mechanisms of leaf development have been well studied in model dicot and monocot species, a lot of information is still needed about the interplay of the genes that regulate cell division and differentiation and thereby affect the photosynthetic performance of the leaf. We were specifically interested in understanding the differentiation of mesophyll and bundle sheath cells in <jats:styled-content style=\"fixed-case\"><jats:italic>Arabidopsis thaliana</jats:italic></jats:styled-content> and aimed to identify genes that are involved in determining bundle sheath anatomy. To this end, we established a forward genetic screen by using ethyl methanesulfonate (EMS) for mutagenizing a reporter line expressing a chloroplast‐targeted green fluorescent protein (sGFP) under the control of a bundle sheath‐specific promoter. Based on the GFP fluorescence phenotype, numerous mutants were produced, and by pursuing a mapping‐by‐sequencing approach, the genomic segments containing mutated candidate genes were identified. One of the lines with an enhanced GFP fluorescence phenotype (named <jats:italic>ELEVATED BUNDLE SHEATH CELLS SIGNAL 1 [ebss1]</jats:italic>) was selected for further study, and the responsible gene was verified by CRISPR/Cas9‐based mutagenesis of candidate genes located in the mapped genomic segment. The verified gene, At2g25970, encodes a K homology (KH) domain‐containing protein.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"83 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140564754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A core of cell wall proteins functions in wall integrity responses in Arabidopsis thaliana","authors":"Oyeyemi Ajayi, Ellen Zelinsky, Charles T. Anderson","doi":"10.1002/pld3.579","DOIUrl":"https://doi.org/10.1002/pld3.579","url":null,"abstract":"Cell walls surround all plant cells, and their composition and structure are tightly regulated to maintain cellular and organismal homeostasis. In response to wall damage, the cell wall integrity (CWI) system is engaged to ameliorate effects on plant growth. Despite the central role CWI plays in plant development, our current understanding of how this system functions at the molecular level is limited. Here, we investigated the transcriptomes of etiolated seedlings of mutants of <jats:styled-content style=\"fixed-case\"><jats:italic>Arabidopsis thaliana</jats:italic></jats:styled-content> with defects in three major wall polysaccharides, pectin (<jats:italic>quasimodo2</jats:italic>), cellulose (<jats:italic>cellulose synthase3</jats:italic><jats:sup><jats:italic>je5</jats:italic></jats:sup>), and xyloglucan (<jats:italic>xyloglucan xylosyltransferase1</jats:italic> and <jats:italic>2</jats:italic>), to probe whether changes in the expression of cell wall‐related genes occur and are similar or different when specific wall components are reduced or missing. Many changes occurred in the transcriptomes of pectin‐ and cellulose‐deficient plants, but fewer changes occurred in the transcriptomes of xyloglucan‐deficient plants. We hypothesize that this might be because pectins interact with other wall components and/or integrity sensors, whereas cellulose forms a major load‐bearing component of the wall; defects in either appear to trigger the expression of structural proteins to maintain wall cohesion in the absence of a major polysaccharide. This core set of genes functioning in CWI in plants represents an attractive target for future genetic engineering of robust and resilient cell walls.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"6 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140564770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antagonistic functions of CTL1 and SUH1 mediate cell wall assembly in Arabidopsis","authors":"Nguyen Thi Thuy, Hyun‐Jung Kim, Suk‐Whan Hong","doi":"10.1002/pld3.580","DOIUrl":"https://doi.org/10.1002/pld3.580","url":null,"abstract":"Plant genomes contain numerous genes encoding chitinase‐like (CTL) proteins, which have a similar protein structure to chitinase belonging to the glycoside hydrolase (GH) family but lack the chitinolytic activity to cleave the <jats:italic>β</jats:italic>‐1,4‐glycosidic bond in chitins, polymers of <jats:italic>N</jats:italic>‐acetylglucosamine. <jats:italic>CTL1</jats:italic> mutations found in rice and <jats:italic>Arabidopsis</jats:italic> have caused pleiotropic developmental defects, including altered cell wall composition and decreased abiotic stress tolerance, likely due to reduced cellulose content. In this study, we identified <jats:italic>suppressor of hot2 1</jats:italic> (<jats:italic>suh1</jats:italic>) as a genetic suppressor of the <jats:italic>ctl1</jats:italic><jats:sup><jats:italic>hot2‐1</jats:italic></jats:sup> mutation in <jats:italic>Arabidopsis</jats:italic>. The mutation in <jats:italic>SUH1</jats:italic> restored almost all examined <jats:italic>ctl1</jats:italic><jats:sup><jats:italic>hot2‐1</jats:italic></jats:sup> defects to nearly wild‐type levels or at least partially. <jats:italic>SUH1</jats:italic> encodes a Golgi‐located type II membrane protein with glycosyltransferase (GT) activity, and its mutations lead to a reduction in cellulose content and hypersensitivity to cellulose biosynthesis inhibitors, although to a lesser extent than <jats:italic>ctl1</jats:italic><jats:sup><jats:italic>hot2‐1</jats:italic></jats:sup> mutation. The <jats:italic>SUH1</jats:italic> promoter fused with the GUS reporter gene exhibited GUS activity in interfascicular fibers and xylem in stems; meanwhile, the <jats:italic>ctl1</jats:italic><jats:sup><jats:italic>hot2‐1</jats:italic></jats:sup> mutation significantly increased this activity. Our findings provide genetic and molecular evidence that the antagonistic activities of CTL1 and SUH1 play an essential role in assembling the cell wall in <jats:italic>Arabidopsis</jats:italic>.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"304 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140199018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Target-site mutations Ile1781Leu and Ile2041Asn in the <i>ACCase2</i> gene confer resistance to fluazifop-p-butyl and pinoxaden herbicides in a johnsongrass accession from Arkansas, USA.","authors":"Fidel González-Torralva, Jason K Norsworthy","doi":"10.1002/pld3.576","DOIUrl":"10.1002/pld3.576","url":null,"abstract":"<p><p>Johnsongrass [<i>Sorghum halepense</i> (L.) Pers.] is a troublesome weed species in different agricultural and non-agricultural areas. Because of its biology, reproductive system, and seed production, effective management is challenging. An accession with low susceptibility to the acetyl-CoA carboxylase (ACCase)-inhibiting herbicides fluazifop-p-butyl (fluazifop) and pinoxaden was collected in eastern Arkansas. In this research, the molecular mechanisms responsible for ACCase resistance were investigated. Dose-response experiments showed a resistance factor of 181 and 133 for fluazifop and pinoxaden, respectively. Molecular analysis of both <i>ACCase1</i> and <i>ACCase2</i> genes was researched. Nucleotide comparison of <i>ACCase1</i> between resistant and susceptible accessions showed no single nucleotide polymorphisms. Nonetheless, analysis of <i>ACCase2</i> in fluazifop-resistant johnsongrass plants revealed the Ile1781Leu target-site mutation was dominant (nearly 75%), whereas the majority of pinoxaden-resistant johnsongrass plants had the Ile2041Asn (60%). Not all sequenced johnsongrass plants displayed a target-site mutation, suggesting the presence of additional resistance mechanisms. Amplification of <i>ACCase1</i> and <i>ACCase2</i> was not responsible for resistance because of the similar values obtained in both resistant and susceptible accessions. Experiments with malathion and NBD-Cl suggest the presence of herbicide metabolism. Outcomes of this research demonstrated that fluazifop- and pinoxaden-resistant johnsongrass plants displayed a target-site mutation in <i>ACCase2</i>, but also that non-target-site resistance mechanisms would be involved and require a detailed study.</p>","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"8 3","pages":"e576"},"PeriodicalIF":2.3,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10955616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140185349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining extracellular matrix proteome and phosphoproteome of chickpea and meta-analysis reveal novel proteoforms and evolutionary significance of clade-specific wall-associated events in plant","authors":"Kanika Narula, Arunima Sinha, Pooja Choudhary, Sudip Ghosh, Eman Elagamey, Archana Sharma, Atreyee Sengupta, Niranjan Chakraborty, Subhra Chakraborty","doi":"10.1002/pld3.572","DOIUrl":"https://doi.org/10.1002/pld3.572","url":null,"abstract":"Extracellular matrix (ECM) plays central roles in cell architecture, innate defense and cell wall integrity (CWI) signaling. During transition to multicellularity, modular domain structures of ECM proteins and proteoforms have evolved due to continuous adaptation across taxonomic clades under different ecological niche. Although this incredible diversity has to some extent been investigated at protein level, extracellular phosphorylation events and molecular evolution of ECM proteoform families remains unexplored. We developed matrisome proteoform atlas in a grain legume, chickpea and performed meta-analyses of 74 plant matrisomes. MS/MS analysis identified 1,424 proteins and 315 phosphoproteins involved in diverse functions. Cross-species ECM protein network identified proteoforms associated with CWI maintenance system. Phylogenetic characterization of eighteen matrix protein families highlighted the role of taxon-specific paralogs and orthologs. Novel information was acquired on gene expansion and loss, co-divergence, sub functionalization and neofunctionalization during evolution. Modular networks of matrix protein families and hub proteins showed higher diversity across taxonomic clades than among organs. Furthermore, protein families differ in nonsynonymous to synonymous substitution rates. Our study pointed towards the matrix proteoform functionality, sequence divergence variation, interactions between wall remodelers and molecular evolution using a phylogenetic framework. This is the first report on comprehensive matrisome proteoform network illustrating presence of CWI signaling proteins in land plants.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"148 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140152965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light quality-dependent roles of REVEILLE proteins in the circadian system.","authors":"Cassandra L Hughes, Yuyan An, Julin N Maloof, Stacey L Harmer","doi":"10.1002/pld3.573","DOIUrl":"10.1002/pld3.573","url":null,"abstract":"<p><p>Several closely related Myb-like activator proteins are known to have partially redundant functions within the plant circadian clock, but their specific roles are not well understood. To clarify the function of the <i>REVEILLE 4</i>, <i>REVEILLE 6</i>, and <i>REVEILLE 8</i> transcriptional activators, we characterized the growth and clock phenotypes of CRISPR-Cas9-generated single, double, and triple <i>rve</i> mutants. We found that these genes act synergistically to regulate flowering time, redundantly to regulate leaf growth, and antagonistically to regulate hypocotyl elongation. We previously reported that increasing intensities of monochromatic blue and red light have opposite effects on the period of triple <i>rve468</i> mutants. Here, we further examined light quality-specific phenotypes of <i>rve</i> mutants and report that <i>rve468</i> mutants lack the blue light-specific increase in expression of some circadian clock genes observed in wild type. To investigate the basis of these blue light-specific circadian phenotypes, we examined RVE protein abundances and degradation rates in blue and red light and found no significant differences between these conditions. We next examined genetic interactions between <i>RVE</i> genes and <i>ZEITLUPE</i> and <i>ELONGATED HYPOCOTYL5</i>, two factors with blue light-specific functions in the clock. We found that the <i>RVEs</i> interact additively with both <i>ZEITLUPE</i> and <i>ELONGATED HYPOCOTYL5</i> to regulate circadian period, which suggests that neither of these factors are required for the blue light-specific differences that we observed. Overall, our results suggest that the <i>RVEs</i> have separable functions in plant growth and circadian regulation and that they are involved in blue light-specific circadian signaling via a novel mechanism.</p>","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"8 3","pages":"e573"},"PeriodicalIF":2.3,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10936234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140120423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of melatonin, proline, and salicylic acid on seedling growth, photosynthetic activity, and leaf nutrients of sorghum under salt stress.","authors":"Mehmet Sait Kiremit, Elif Öztürk, Hakan Arslan, Bhaskara Anggarda Gathot Subrata, Hasan Akay, Aigerim Bakirova","doi":"10.1002/pld3.574","DOIUrl":"10.1002/pld3.574","url":null,"abstract":"<p><p>Soil salinization poses a significant challenge to the sustainability and productivity of agriculture worldwide. This issue continues to hinder plant growth, requiring innovative solutions to alleviate salt stress. Moreover, climate change accelerates soil salinization, which may soon spread to previously unaffected agricultural areas. Therefore, the present study evaluated the potential role of different seed priming agents (hydro (H), salicylic acid (SA), proline (P), and melatonin (MEL)) on seedlings and leaf macro and micronutrients of sorghum grown under four (.27, 2.5, 5.0, and 8.0 dS m^-1) soil salinity conditions. Soil salinity drastically reduced all the growth parameters of sorghum seedlings, primarily the reduction in growth traits, which was remarkable after 2.5 dS m^-1 soil salinity. In addition, plant height, shoot fresh weight, and stomata were reduced by 40.8%, 74.6%, and 36.5%, respectively, at 8.0 dS m^-1 compared to .27 dS m^-1. SA- and MEL-primed seeds mitigated the harmful effects of soil salinity by reducing Na⁺ accumulation in the leaves and increasing the K⁺/Na⁺ and Ca²⁺/Na⁺ ratios and photosynthetic activity under salt stress. However, the Zn²⁺, Mn²⁺, and Cu²⁺ contents of sorghum leaves increased with increasing soil salinity, and these nutrients also improved with seed priming by SA, MEL, and P. Considering all nutrients, MEL-primed sorghum seeds had better macro- and micro-nutrient uptake capacities than the H, SA, and P treatments under high soil salinity conditions. Finally, the present study showed that MEL-induced improvement in salt tolerance in sorghum seedlings was related to enhanced nutritional status, photosynthetic activity, and biomass production in salinized areas.</p>","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"8 3","pages":"e574"},"PeriodicalIF":2.3,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10933660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140120388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosome-scale genome assembly of <i>Poa trivialis</i> and population genomics reveal widespread gene flow in a cool-season grass seed production system.","authors":"Caio A C G Brunharo, Christopher W Benson, David R Huff, Jesse R Lasky","doi":"10.1002/pld3.575","DOIUrl":"10.1002/pld3.575","url":null,"abstract":"<p><p><i>Poa trivialis</i> (L.) is a cool-season grass species found in various environments worldwide. In addition to being a desired turfgrass species, it is a common weed of agricultural systems and natural areas. As a weed, it is an important contaminant of commercial cool-season grass seed lots, resulting in widespread gene flow facilitated by human activities and causing significant economic losses to farmers. To better understand and manage infestations, we assembled and annotated a haploid genome of <i>P. trivialis</i> and studied troublesome field populations from Oregon, the largest cool-season grass seed producing region in the United States. The genome assembly resulted in 1.35 Gb of DNA sequence distributed among seven chromosome-scale scaffolds, revealing a high content of transposable elements, conserved synteny with <i>Poa annua</i>, and a close relationship with other C₃ grasses. A reduced-representation sequencing analysis of field populations revealed limited genetic diversity and suggested potential gene flow and human-assisted dispersal in the region. The genetic resources and insights into <i>P. trivialis</i> provided by this study will improve weed management strategies and enable the development of molecular detection tests for contaminated seed lots to limit seed-mediated gene flow. These resources should also be beneficial for turfgrass breeders seeking to improve desirable traits of commercial <i>P. trivialis</i> varieties and help to guide breeding efforts in other crops to enhance the resiliency of agricultural ecosystems under climate change. Significance Statement: The chromosome-scale assembly of Poa trivialis and population genomic analyses provide crucial insights into the gene flow of weedy populations in agricultural systems and contribute a valuable genomic resource for the plant science community.</p>","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"8 3","pages":"e575"},"PeriodicalIF":2.3,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10934236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140120387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non‐destructive, whole‐plant phenotyping reveals dynamic changes in water use efficiency, photosynthesis, and rhizosphere acidification of sorghum accessions under osmotic stress","authors":"Daniel N. Ginzburg, Jack A. Cox, Seung Y. Rhee","doi":"10.1002/pld3.571","DOIUrl":"https://doi.org/10.1002/pld3.571","url":null,"abstract":"Noninvasive phenotyping can quantify dynamic plant growth processes at higher temporal resolution than destructive phenotyping and can reveal phenomena that would be missed by end‐point analysis alone. Additionally, whole‐plant phenotyping can identify growth conditions that are optimal for both above‐ and below‐ground tissues. However, noninvasive, whole‐plant phenotyping approaches available today are generally expensive, complex, and non‐modular. We developed a low‐cost and versatile approach to noninvasively measure whole‐plant physiology over time by growing plants in isolated hydroponic chambers. We demonstrate the versatility of our approach by measuring whole‐plant biomass accumulation, water use, and water use efficiency every two days on unstressed and osmotically stressed sorghum accessions. We identified relationships between root zone acidification and photosynthesis on whole‐plant water use efficiency over time. Our system can be implemented using cheap, basic components, requires no specific technical expertise, and should be suitable for any non‐aquatic vascular plant species.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"75 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140076093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}