Plant Direct最新文献_第6页

Combining extracellular matrix proteome and phosphoproteome of chickpea and meta-analysis reveal novel proteoforms and evolutionary significance of clade-specific wall-associated events in plant 结合鹰嘴豆的细胞外基质蛋白质组和磷酸蛋白质组以及荟萃分析，揭示植物中新的蛋白质形式和支系特异性壁相关事件的进化意义

IF 3 3区生物学

Plant Direct Pub Date : 2024-03-18 DOI: 10.1002/pld3.572

Kanika Narula, Arunima Sinha, Pooja Choudhary, Sudip Ghosh, Eman Elagamey, Archana Sharma, Atreyee Sengupta, Niranjan Chakraborty, Subhra Chakraborty

{"title":"Combining extracellular matrix proteome and phosphoproteome of chickpea and meta-analysis reveal novel proteoforms and evolutionary significance of clade-specific wall-associated events in plant","authors":"Kanika Narula, Arunima Sinha, Pooja Choudhary, Sudip Ghosh, Eman Elagamey, Archana Sharma, Atreyee Sengupta, Niranjan Chakraborty, Subhra Chakraborty","doi":"10.1002/pld3.572","DOIUrl":"https://doi.org/10.1002/pld3.572","url":null,"abstract":"Extracellular matrix (ECM) plays central roles in cell architecture, innate defense and cell wall integrity (CWI) signaling. During transition to multicellularity, modular domain structures of ECM proteins and proteoforms have evolved due to continuous adaptation across taxonomic clades under different ecological niche. Although this incredible diversity has to some extent been investigated at protein level, extracellular phosphorylation events and molecular evolution of ECM proteoform families remains unexplored. We developed matrisome proteoform atlas in a grain legume, chickpea and performed meta-analyses of 74 plant matrisomes. MS/MS analysis identified 1,424 proteins and 315 phosphoproteins involved in diverse functions. Cross-species ECM protein network identified proteoforms associated with CWI maintenance system. Phylogenetic characterization of eighteen matrix protein families highlighted the role of taxon-specific paralogs and orthologs. Novel information was acquired on gene expansion and loss, co-divergence, sub functionalization and neofunctionalization during evolution. Modular networks of matrix protein families and hub proteins showed higher diversity across taxonomic clades than among organs. Furthermore, protein families differ in nonsynonymous to synonymous substitution rates. Our study pointed towards the matrix proteoform functionality, sequence divergence variation, interactions between wall remodelers and molecular evolution using a phylogenetic framework. This is the first report on comprehensive matrisome proteoform network illustrating presence of CWI signaling proteins in land plants.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140152965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light quality-dependent roles of REVEILLE proteins in the circadian system. REVEILLE 蛋白在昼夜节律系统中的作用取决于光质。

IF 2.3 3区生物学

Plant Direct Pub Date : 2024-03-13 eCollection Date: 2024-03-01 DOI: 10.1002/pld3.573

Cassandra L Hughes, Yuyan An, Julin N Maloof, Stacey L Harmer

{"title":"Light quality-dependent roles of REVEILLE proteins in the circadian system.","authors":"Cassandra L Hughes, Yuyan An, Julin N Maloof, Stacey L Harmer","doi":"10.1002/pld3.573","DOIUrl":"10.1002/pld3.573","url":null,"abstract":"Several closely related Myb-like activator proteins are known to have partially redundant functions within the plant circadian clock, but their specific roles are not well understood. To clarify the function of the REVEILLE 4, REVEILLE 6, and REVEILLE 8 transcriptional activators, we characterized the growth and clock phenotypes of CRISPR-Cas9-generated single, double, and triple rve mutants. We found that these genes act synergistically to regulate flowering time, redundantly to regulate leaf growth, and antagonistically to regulate hypocotyl elongation. We previously reported that increasing intensities of monochromatic blue and red light have opposite effects on the period of triple rve468 mutants. Here, we further examined light quality-specific phenotypes of rve mutants and report that rve468 mutants lack the blue light-specific increase in expression of some circadian clock genes observed in wild type. To investigate the basis of these blue light-specific circadian phenotypes, we examined RVE protein abundances and degradation rates in blue and red light and found no significant differences between these conditions. We next examined genetic interactions between RVE genes and ZEITLUPE and ELONGATED HYPOCOTYL5, two factors with blue light-specific functions in the clock. We found that the RVEs interact additively with both ZEITLUPE and ELONGATED HYPOCOTYL5 to regulate circadian period, which suggests that neither of these factors are required for the blue light-specific differences that we observed. Overall, our results suggest that the RVEs have separable functions in plant growth and circadian regulation and that they are involved in blue light-specific circadian signaling via a novel mechanism.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10936234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140120423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of melatonin, proline, and salicylic acid on seedling growth, photosynthetic activity, and leaf nutrients of sorghum under salt stress. 褪黑素、脯氨酸和水杨酸对盐胁迫下高粱幼苗生长、光合作用活性和叶片养分的影响

IF 2.3 3区生物学

Plant Direct Pub Date : 2024-03-13 eCollection Date: 2024-03-01 DOI: 10.1002/pld3.574

Mehmet Sait Kiremit, Elif Öztürk, Hakan Arslan, Bhaskara Anggarda Gathot Subrata, Hasan Akay, Aigerim Bakirova

{"title":"Effects of melatonin, proline, and salicylic acid on seedling growth, photosynthetic activity, and leaf nutrients of sorghum under salt stress.","authors":"Mehmet Sait Kiremit, Elif Öztürk, Hakan Arslan, Bhaskara Anggarda Gathot Subrata, Hasan Akay, Aigerim Bakirova","doi":"10.1002/pld3.574","DOIUrl":"10.1002/pld3.574","url":null,"abstract":"Soil salinization poses a significant challenge to the sustainability and productivity of agriculture worldwide. This issue continues to hinder plant growth, requiring innovative solutions to alleviate salt stress. Moreover, climate change accelerates soil salinization, which may soon spread to previously unaffected agricultural areas. Therefore, the present study evaluated the potential role of different seed priming agents (hydro (H), salicylic acid (SA), proline (P), and melatonin (MEL)) on seedlings and leaf macro and micronutrients of sorghum grown under four (.27, 2.5, 5.0, and 8.0 dS m-1) soil salinity conditions. Soil salinity drastically reduced all the growth parameters of sorghum seedlings, primarily the reduction in growth traits, which was remarkable after 2.5 dS m-1 soil salinity. In addition, plant height, shoot fresh weight, and stomata were reduced by 40.8%, 74.6%, and 36.5%, respectively, at 8.0 dS m-1 compared to .27 dS m-1. SA- and MEL-primed seeds mitigated the harmful effects of soil salinity by reducing Na+ accumulation in the leaves and increasing the K+/Na+ and Ca2+/Na+ ratios and photosynthetic activity under salt stress. However, the Zn2+, Mn2+, and Cu2+ contents of sorghum leaves increased with increasing soil salinity, and these nutrients also improved with seed priming by SA, MEL, and P. Considering all nutrients, MEL-primed sorghum seeds had better macro- and micro-nutrient uptake capacities than the H, SA, and P treatments under high soil salinity conditions. Finally, the present study showed that MEL-induced improvement in salt tolerance in sorghum seedlings was related to enhanced nutritional status, photosynthetic activity, and biomass production in salinized areas.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10933660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140120388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromosome-scale genome assembly of Poa trivialis and population genomics reveal widespread gene flow in a cool-season grass seed production system. Poa trivialis 染色体尺度基因组组装和种群基因组学揭示了冷季型草籽生产系统中广泛的基因流动。

IF 2.3 3区生物学

Plant Direct Pub Date : 2024-03-13 eCollection Date: 2024-03-01 DOI: 10.1002/pld3.575

Caio A C G Brunharo, Christopher W Benson, David R Huff, Jesse R Lasky

{"title":"Chromosome-scale genome assembly of Poa trivialis and population genomics reveal widespread gene flow in a cool-season grass seed production system.","authors":"Caio A C G Brunharo, Christopher W Benson, David R Huff, Jesse R Lasky","doi":"10.1002/pld3.575","DOIUrl":"10.1002/pld3.575","url":null,"abstract":"Poa trivialis (L.) is a cool-season grass species found in various environments worldwide. In addition to being a desired turfgrass species, it is a common weed of agricultural systems and natural areas. As a weed, it is an important contaminant of commercial cool-season grass seed lots, resulting in widespread gene flow facilitated by human activities and causing significant economic losses to farmers. To better understand and manage infestations, we assembled and annotated a haploid genome of P. trivialis and studied troublesome field populations from Oregon, the largest cool-season grass seed producing region in the United States. The genome assembly resulted in 1.35 Gb of DNA sequence distributed among seven chromosome-scale scaffolds, revealing a high content of transposable elements, conserved synteny with Poa annua, and a close relationship with other C3 grasses. A reduced-representation sequencing analysis of field populations revealed limited genetic diversity and suggested potential gene flow and human-assisted dispersal in the region. The genetic resources and insights into P. trivialis provided by this study will improve weed management strategies and enable the development of molecular detection tests for contaminated seed lots to limit seed-mediated gene flow. These resources should also be beneficial for turfgrass breeders seeking to improve desirable traits of commercial P. trivialis varieties and help to guide breeding efforts in other crops to enhance the resiliency of agricultural ecosystems under climate change. Significance Statement: The chromosome-scale assembly of Poa trivialis and population genomic analyses provide crucial insights into the gene flow of weedy populations in agricultural systems and contribute a valuable genomic resource for the plant science community.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10934236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140120387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non‐destructive, whole‐plant phenotyping reveals dynamic changes in water use efficiency, photosynthesis, and rhizosphere acidification of sorghum accessions under osmotic stress 非破坏性全株表型揭示渗透胁迫下高粱品种水分利用效率、光合作用和根圈酸化的动态变化

IF 3 3区生物学

Plant Direct Pub Date : 2024-03-07 DOI: 10.1002/pld3.571

Daniel N. Ginzburg, Jack A. Cox, Seung Y. Rhee

{"title":"Non‐destructive, whole‐plant phenotyping reveals dynamic changes in water use efficiency, photosynthesis, and rhizosphere acidification of sorghum accessions under osmotic stress","authors":"Daniel N. Ginzburg, Jack A. Cox, Seung Y. Rhee","doi":"10.1002/pld3.571","DOIUrl":"https://doi.org/10.1002/pld3.571","url":null,"abstract":"Noninvasive phenotyping can quantify dynamic plant growth processes at higher temporal resolution than destructive phenotyping and can reveal phenomena that would be missed by end‐point analysis alone. Additionally, whole‐plant phenotyping can identify growth conditions that are optimal for both above‐ and below‐ground tissues. However, noninvasive, whole‐plant phenotyping approaches available today are generally expensive, complex, and non‐modular. We developed a low‐cost and versatile approach to noninvasively measure whole‐plant physiology over time by growing plants in isolated hydroponic chambers. We demonstrate the versatility of our approach by measuring whole‐plant biomass accumulation, water use, and water use efficiency every two days on unstressed and osmotically stressed sorghum accessions. We identified relationships between root zone acidification and photosynthesis on whole‐plant water use efficiency over time. Our system can be implemented using cheap, basic components, requires no specific technical expertise, and should be suitable for any non‐aquatic vascular plant species.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140076093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and expression of strigolactone biosynthesis and signaling genes and the in vitro effects of strigolactones in olive (Olea europaea L.) 橄榄（Olea europaea L.）中绞股蓝内酯生物合成和信号转导基因的鉴定与表达以及绞股蓝内酯的体外效应

IF 3 3区生物学

Plant Direct Pub Date : 2024-02-25 DOI: 10.1002/pld3.568

Aslıhan Özbilen, Fatih Sezer, Kemal Melih Taşkin

{"title":"Identification and expression of strigolactone biosynthesis and signaling genes and the in vitro effects of strigolactones in olive (Olea europaea L.)","authors":"Aslıhan Özbilen, Fatih Sezer, Kemal Melih Taşkin","doi":"10.1002/pld3.568","DOIUrl":"https://doi.org/10.1002/pld3.568","url":null,"abstract":"Strigolactones (SLs), synthesized in plant roots, play a dual role in modulating plant growth and development, and in inducing the germination of parasitic plant seeds and arbuscular mycorrhizal fungi in the rhizosphere. As phytohormones, SLs are crucial in regulating branching and shaping plant architecture. Despite the significant impact of branching strategies on the yield performance of fruit crops, limited research has been conducted on SLs in these crops. In our study, we identified the transcript sequences of SL biosynthesis and signaling genes in olive (Olea europaea L.) using rapid amplification of cDNA ends. We predicted the corresponding protein sequences, analyzed their characteristics, and conducted molecular docking with bioinformatics tools. Furthermore, we quantified the expression levels of these genes in various tissues using quantitative real-time PCR. Our findings demonstrate the predominant expression of SL biosynthesis and signaling genes (OeD27, OeMAX3, OeMAX4, OeMAX1, OeD14, and OeMAX2) in roots and lateral buds, highlighting their importance in branching. Treatment with rac-GR24, an SL analog, enhanced the germination frequency of olive seeds in vitro compared with untreated embryos. Conversely, inhibition of SL biosynthesis with TIS108 increased lateral bud formation in a hard-to-root cultivar, underscoring the role of SLs as phytohormones in olives. These results suggest that modifying SL biosynthesis and signaling pathways could offer novel approaches for olive breeding, with potential applicability to other fruit crops.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139969717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A fibrous scaffold for in vitro culture and experimental studies of Physcomitrium patens 体外培养和实验研究用的纤维支架

IF 3 3区生物学

Plant Direct Pub Date : 2024-02-19 DOI: 10.1002/pld3.570

Ryan Calcutt, Yasaman Aghli, Treena Arinzeh, Ram Dixit

{"title":"A fibrous scaffold for in vitro culture and experimental studies of Physcomitrium patens","authors":"Ryan Calcutt, Yasaman Aghli, Treena Arinzeh, Ram Dixit","doi":"10.1002/pld3.570","DOIUrl":"https://doi.org/10.1002/pld3.570","url":null,"abstract":"The model moss, Physcomitrium patens, is routinely cultured on cellophane placed over a solid nutrient medium. While this culture method is convenient for moss propagation, it is not suitable for studying how topographical features and mechanical cues from the environment influence the growth and development of moss. Here, we show that P. patens can be grown on fibrous scaffolds consisting of nanoscale, randomly oriented fibers composed of polyvinylidene tri-fluoroethylene (NRP). The moss adheres tightly to NRP in contrast to the lack of adhesion to cellophane. Adhesion to the scaffold is associated with slower tip growth of moss protonema for some time, followed by an increase in tip growth rate that is equivalent to that on cellophane. In addition, the orientation of the first subapical cell division plane differs between NRP-grown and cellophane-grown protonema. Nonetheless, moss colonies grown on NRP did not show signs of nutrient or photosynthetic stress and developed normal gametophores. Together, these data establish NRP as a suitable substrate for the culture of P. patens and to probe the influence of mechanical forces on tip growth and cell division of moss.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139921820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The melon Fom-1–Prv resistance gene pair: Correlated spatial expression and interaction with a viral protein 甜瓜 Fom-1-Prv 抗性基因对：相关空间表达以及与病毒蛋白的相互作用

IF 3 3区生物学

Plant Direct Pub Date : 2024-02-14 DOI: 10.1002/pld3.565

Michael Normantovich, Arie Amitzur, Sharon Offri, Ekaterina Pashkovsky, Yula Shnaider, Shahar Nizan, Ohad Yogev, Avi Jacob, Christopher G. Taylor, Cécile Desbiez, Steven A. Whitham, Amalia Bar-Ziv, Rafael Perl-Treves

{"title":"The melon Fom-1–Prv resistance gene pair: Correlated spatial expression and interaction with a viral protein","authors":"Michael Normantovich, Arie Amitzur, Sharon Offri, Ekaterina Pashkovsky, Yula Shnaider, Shahar Nizan, Ohad Yogev, Avi Jacob, Christopher G. Taylor, Cécile Desbiez, Steven A. Whitham, Amalia Bar-Ziv, Rafael Perl-Treves","doi":"10.1002/pld3.565","DOIUrl":"https://doi.org/10.1002/pld3.565","url":null,"abstract":"The head-to-head oriented pair of melon resistance genes, Fom-1 and Prv, control resistance to Fusarium oxysporum races 0 and 2 and papaya ringspot virus (PRSV), respectively. They encode, via several RNA splice variants, TIR-NBS-LRR proteins, and Prv has a C-terminal extra domain with a second NBS homologous sequence. In other systems, paired R-proteins were shown to operate by “labor division,” with one protein having an extra integrated domain that directly binds the pathogen's Avr factor, and the second protein executing the defense response. We report that the expression of the two genes in two pairs of near-isogenic lines was higher in the resistant isoline and inducible by F. oxysporum race 2 but not by PRSV. The intergenic DNA region separating the coding sequences of the two genes acted as a bi-directional promoter and drove GUS expression in transgenic melon roots and transgenic tobacco plants. Expression of both genes was strong in melon root tips, around the root vascular cylinder, and the phloem and xylem parenchyma of tobacco stems and petioles. The pattern of GUS expression suggests coordinated expression of the two genes. In agreement with the above model, Prv's extra domain was shown to interact with the cylindrical inclusion protein of PRSV both in yeast cells and in planta.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139767929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The maize striate leaves2 (sr2) gene encodes a conserved DUF3732 domain and is homologous to the rice yss1 gene. 玉米条纹叶2（sr2）基因编码一个保守的DUF3732结构域，与水稻yss1基因同源。

IF 2.3 3区生物学

Plant Direct Pub Date : 2024-02-13 eCollection Date: 2024-02-01 DOI: 10.1002/pld3.567

Meghan J Brady, Maya Cheam, Jonathan I Gent, R Kelly Dawe

{"title":"The maize striate leaves2 (sr2) gene encodes a conserved DUF3732 domain and is homologous to the rice yss1 gene.","authors":"Meghan J Brady, Maya Cheam, Jonathan I Gent, R Kelly Dawe","doi":"10.1002/pld3.567","DOIUrl":"10.1002/pld3.567","url":null,"abstract":"Maize striate leaves2 (sr2) is a mutant that causes white stripes on leaves that has been used in mapping studies for decades though the underlying gene has not been identified. The sr2 locus has been previously mapped to small regions of normal chromosome 10 (N10) and a rearranged variant called abnormal chromosome 10 (Ab10). A comparison of assembled genomes carrying N10 and Ab10 revealed only five candidate sr2 genes. Analysis of a stock carrying the sr2 reference allele (sr2-ref) showed that one of the five genes has a transposon insertion that disrupts its protein sequence and has a severe reduction in mRNA. An independent Mutator transposon insertion in the gene (sr2-Mu) failed to complement the sr2-ref mutation, and plants homozygous for sr2-Mu showed white striped leaf margins. The sr2 gene encodes a DUF3732 protein with strong homology to a rice gene with a similar mutant phenotype called young seedling stripe1 (yss1). These and other published data suggest that sr2 may have a function in plastid gene expression.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10864124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139735913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salinity inhibits seed germination and embryo growth by reducing starch mobilization efficiency in barley 盐分通过降低大麦的淀粉动员效率抑制种子萌发和胚胎生长

IF 3 3区生物学

Plant Direct Pub Date : 2024-02-02 DOI: 10.1002/pld3.564

Min Xiong, Jian Xu, Zhou Zhou, Bin Peng, Yuxiang Shen, Huiquan Shen, Xiao Xu, Changya Li, Lina Deng, Gongneng Feng

{"title":"Salinity inhibits seed germination and embryo growth by reducing starch mobilization efficiency in barley","authors":"Min Xiong, Jian Xu, Zhou Zhou, Bin Peng, Yuxiang Shen, Huiquan Shen, Xiao Xu, Changya Li, Lina Deng, Gongneng Feng","doi":"10.1002/pld3.564","DOIUrl":"https://doi.org/10.1002/pld3.564","url":null,"abstract":"Barley is one of the world's earliest domesticated crops, which is widely used for beer production, animal feeding, and health care. Barley seed germination, particularly in increasingly saline soils, is key to ensure the safety of crop production. However, the mechanism of salt-affected seed germination in barley remains elusive. Here, two different colored barley varieties were used to independently study the regulation mechanism of salt tolerance during barley seed germination. High salinity delays barley seed germination by slowing down starch mobilization efficiency in seeds. The starch plate test revealed that salinity had a significant inhibitory effect on α-amylase activity in barley seeds. Further, NaCl treatment down-regulated the expression of Amy1, Amy2 and Amy3 genes in germinated seeds, thereby inhibiting α-amylase activity. In addition, the result of embryogenic culture system in vitro showed that the shoot elongation of barley was significantly inhibited by salt stress. These findings indicate that it is a feasible idea to study the regulation mechanism of salinity on barley seed germination and embryo growth from the aspect of starch-related source-sink communication.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139667386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0