Plant Direct最新文献

{"title":"Intraspecific differences in the photosynthetic responses to chloroplast CO₂ and photon flux density at different leaf temperatures of four grapevine cultivars grown in common outdoor conditions.","authors":"Dennis H Greer","doi":"10.1002/pld3.595","DOIUrl":"10.1002/pld3.595","url":null,"abstract":"<p><p>Comparative measurements of four <i>Vitis vinifera</i> cultivars were undertaken to assess assimilation tolerance to the high growth temperatures currently pervading Australian and other wine growing regions. The cultivars, cvs. Chardonnay, Merlot, Semillon, and Shiraz, were all grown in common growth conditions, and an hypothesis promulgated genotypic variation in assimilation and in the leaf temperature dependency. Assimilation responses to varying light intensity and to varying chloroplast CO₂ at a range of leaf temperatures (15-45°C) were measured in leaves of each cultivar in mid-summer. Light response curves revealed marked genotype differences in maximum assimilation, but temperature effects also varied. Semillon leaves were most sensitive to temperature, with marked and steep differences in assimilation at different temperatures while Chardonnay and Merlot were least sensitive, with relatively flat responses. There were also marked cultivar differences in response to CO₂ and significant effects of leaf temperature. CO₂-saturated assimilation varied markedly, with Semillon and Merlot leaves most responsive to temperature, although there were differences in optimum temperatures and maximum rates. Chardonnay leaves remained least tolerant, with lowest rates of assimilation across most temperatures. Assimilation at 45°C also separated the cultivars and two cultivars had higher rates than at 15°C while Chardonnay and Merlot leaves had higher rates at 15°C. There were no cultivar differences in the temperature dependency of Ribulose 1,5-bisphosphate (RuBP) carboxylation, but Semillon had a much steeper temperature dependency on RuBP regeneration than the other cultivars. All these responses confirmed the hypothesis and concluded the high-temperature tolerance of Semillon and Shiraz and the poor adaptability of Chardonnay and possibly Merlot to perform in the current high-temperature growth conditions.</p>","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"8 6","pages":"e595"},"PeriodicalIF":3.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11154808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Critical parameters for robust <i>Agrobacterium</i>-mediated transient transformation and quantitative promoter assays in <i>Catharanthus roseus</i> seedlings.","authors":"Lauren F Cole-Osborn, Emma Meehan, Carolyn W T Lee-Parsons","doi":"10.1002/pld3.596","DOIUrl":"10.1002/pld3.596","url":null,"abstract":"<p><p><i>Agrobacterium</i>-mediated transient expression methods are widely used to study gene function in both model and non-model plants. Using a dual-luciferase assay, we quantified the effect of <i>Agrobacterium</i>-infiltration parameters on the transient transformation efficiency of <i>Catharanthus roseus</i> seedlings. We showed that transformation efficiency is highly sensitive to seedling developmental state and a pre- and post-infiltration dark incubation and is less sensitive to the <i>Agrobacterium</i> growth stage. For example, 5 versus 6 days of germination in the dark increased seedling transformation efficiency by seven- to eight-fold while a dark incubation pre- and post-infiltration increased transformation efficiency by five- to 13-fold. <i>Agrobacterium</i> in exponential compared with stationary phase increased transformation efficiency by two-fold. Finally, we quantified the variation in our <i>Agrobacterium</i>-infiltration method in replicate infiltrations and experiments. Within a given experiment, significant differences of up to 2.6-fold in raw firefly luciferase (<i>FLUC</i>) and raw <i>Renilla</i> luciferase (<i>RLUC</i>) luminescence occurred in replicate infiltrations. These differences were significantly reduced when FLUC was normalized to RLUC values, highlighting the utility of including a reference reporter to minimize false positives. Including a second experimental replicate further reduced the potential for false positives. This optimization and quantitative validation of <i>Agrobacterium</i> infiltration in <i>C. roseus</i> seedlings will facilitate the study of this important medicinal plant and will expand the application of <i>Agrobacterium</i>-mediated transformation methods in other plant species.</p>","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"8 6","pages":"e596"},"PeriodicalIF":3.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11154794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional repression of GTL1 under water‐deficit stress promotes anthocyanin biosynthesis to enhance drought tolerance","authors":"Noel Anthony Mano, Mearaj A. Shaikh, Joshua R. Widhalm, Chan Yul Yoo, Michael V. Mickelbart","doi":"10.1002/pld3.594","DOIUrl":"https://doi.org/10.1002/pld3.594","url":null,"abstract":"The transcription factor GT2‐LIKE 1 (GTL1) has been implicated in orchestrating a transcriptional network of diverse physiological, biochemical, and developmental processes. In response to water‐limiting conditions, GTL1 is a negative regulator of stomatal development, but its potential rolein other water‐deficit responses is unknown. We hypothesized that GTL1 regulates transcriptome changes associated with drought tolerance over leaf developmental stages. To test the hypothesis, gene expression was profiled by RNA‐seq analysis in emerging and expanding leaves of wild‐type and a drought‐tolerant <jats:italic>gtl1‐4</jats:italic> knockout mutant under well‐watered and water‐deficit conditions. Our comparative analysis of genotype‐treatment combinations within leaf developmental age identified 459 and 1073 differentially expressed genes in emerging and expanding leaves, respectively, as water‐deficit responsive GTL1‐regulated genes. Transcriptional profiling identified a potential role of GTL1 in two important pathways previously linked to drought tolerance: flavonoid and polyamine biosynthesis. In expanding leaves, negative regulation of <jats:italic>GTL1</jats:italic> under water‐deficit conditions promotes biosynthesis of flavonoids and anthocyanins that may contribute to drought tolerance. Quantification of polyamines did not support a role for GTL1 in these drought‐responsive pathways, but this is likely due to the complex nature of polyamine synthesis and turnover. Our global transcriptome analysis suggests that transcriptional repression of GTL1 by water deficit allows plants to activate diverse pathways that collectively contribute to drought tolerance.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"162 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141149339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis of the defense response in \"Cabernet Sauvignon\" grape leaf induced by <i>Apolygus lucorum</i> feeding.","authors":"Heng Yao, Suhong Gao, Tianhua Sun, Guona Zhou, Changkuan Lu, Baojia Gao, Wenshu Chen, Yiming Liang","doi":"10.1002/pld3.590","DOIUrl":"10.1002/pld3.590","url":null,"abstract":"<p><p>To investigate the molecular mechanism of the defense response of \"Cabernet Sauvignon\" grapes to feeding by <i>Apolygus lucorum</i>, high-throughput sequencing technology was used to analyze the transcriptome of grape leaves under three different treatments: feeding by <i>A. lucorum</i>, puncture injury, and an untreated control. The research findings indicated that the differentially expressed genes were primarily enriched in three aspects: cellular composition, molecular function, and biological process. These genes were found to be involved in 42 metabolic pathways, particularly in plant hormone signaling metabolism, plant-pathogen interaction, MAPK signaling pathway, and other metabolic pathways associated with plant-induced insect resistance. Feeding by <i>A. lucorum</i> stimulated and upregulated a significant number of genes related to jasmonic acid and calcium ion pathways, suggesting their crucial role in the defense molecular mechanism of \"Cabernet Sauvignon\" grapes. The consistency between the gene expression and transcriptome sequencing results further supports these findings. This study provides a reference for the further exploration of the defense response in \"Cabernet Sauvignon\" grapes by elucidating the expression of relevant genes during feeding by <i>A. lucorum</i>.</p>","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"8 5","pages":"e590"},"PeriodicalIF":2.3,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11108798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141080828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Project ChemicalBlooms: Collaborating with citizen scientists to survey the chemical diversity and phylogenetic distribution of plant epicuticular wax blooms","authors":"Le Thanh Dien Nguyen, Nicole Groth, Kylie Mondloch, Edgar B. Cahoon, Keith Jones, Lucas Busta","doi":"10.1002/pld3.588","DOIUrl":"https://doi.org/10.1002/pld3.588","url":null,"abstract":"Plants use chemistry to overcome diverse challenges. A particularly striking chemical trait that some plants possess is the ability to synthesize massive amounts of epicuticular wax that accumulates on the plant's surfaces as a white coating visible to the naked eye. The ability to synthesize basic wax molecules appears to be shared among virtually all land plants, and our knowledge of ubiquitous wax compound synthesis is reasonably advanced. However, the ability to synthesize thick layers of visible epicuticular crystals (“wax blooms”) is restricted to specific lineages, and our knowledge of how wax blooms differ from ubiquitous wax layers is less developed. Here, we recruited the help of citizen scientists and middle school students to survey the wax bloom chemistry of 78 species spanning dicot, monocot, and gymnosperm lineages. Using gas chromatography–mass spectrometry, we found that the major wax classes reported from bulk wax mixtures can be present in wax bloom crystals, with fatty acids, fatty alcohols, and alkanes being present in many species' bloom crystals. In contrast, other compounds including aldehydes, ketones, secondary alcohols, and triterpenoids were present in only a few species' wax bloom crystals. By mapping the 78 wax bloom chemical profiles onto a phylogeny and using phylogenetic comparative analyses, we found that secondary alcohol and triterpenoid‐rich wax blooms were present in lineage‐specific patterns that would not be expected to arise by chance. That finding is consistent with reports that secondary alcohol biosynthesis enzymes are found only in certain lineages but was a surprise for triterpenoids, which are intracellular components in virtually all plant lineages. Thus, our data suggest that a lineage‐specific mechanism other than biosynthesis exists that enables select species to generate triterpenoid‐rich surface wax crystals. Overall, our study outlines a general mode in which research scientists can collaborate with citizen scientists as well as middle and high school classrooms not only to enhance data collection and generate testable hypotheses but also to directly involve classrooms in the scientific process and inspire future STEM workers.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"15 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141063299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phaseolus vulgaris STP13.1 is an H+‐coupled monosaccharide transporter, present in source leaves and seed coats, with higher substrate affinity at depolarized potentials","authors":"Joseph L. Pegler, John W. Patrick, Benjamin McDermott, Anthony Brown, Jackson M. J. Oultram, Christopher P. L. Grof, John M. Ward","doi":"10.1002/pld3.585","DOIUrl":"https://doi.org/10.1002/pld3.585","url":null,"abstract":"Sugar transport proteins (STPs) are high‐affinity H<jats:sup>+</jats:sup>‐coupled hexose symporters. Recently, the contribution of STP13 to bacterial and fungal pathogen resistance across multiple plant species has garnered significant interest. Quantitative PCR analysis of source leaves, developing embryos, and seed coats of <jats:styled-content style=\"fixed-case\"><jats:italic>Phaseolus vulgaris</jats:italic></jats:styled-content> <jats:italic>L</jats:italic>. (common bean) revealed that <jats:italic>PvSTP13.1</jats:italic> was expressed in source leaves and seed coats throughout seed development. In contrast, <jats:italic>PvSTP13.1</jats:italic> transcripts were detected at exceedingly low levels in developing embryos. To characterize the transport mechanism, PvSTP13.1 was expressed in <jats:styled-content style=\"fixed-case\"><jats:italic>Xenopus laevis</jats:italic></jats:styled-content> oocytes, and inward‐directed currents were analyzed using two‐electrode voltage clamping. PvSTP13.1 was shown to function as an H<jats:sup>+</jats:sup>‐coupled monosaccharide symporter exhibiting a unique high affinity for hexoses and aldopentoses at depolarized membrane potentials. Specifically, of the 31 assessed substrates, which included aldohexoses, deoxyhexoses, fructose, 3‐O‐methyl‐D‐glucose, aldopentoses, polyols, glycosides, disaccharides, trisaccharides, and glucuronic acid, PvSTP13.1 displayed the highest affinity (<jats:italic>K</jats:italic><jats:sub>0.5</jats:sub>) for glucose (43 μM), mannose (92 μM), galactose (145 μM), fructose (224 μM), xylose (1.0 mM), and fucose (3.7 mM) at pH 5.6 at a depolarized membrane potential of −40 mV. The results presented here suggest PvSTP13.1 contributes to retrieval of hexoses from the apoplasmic space in source leaves and coats of developing seeds.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"52 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140634924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pectin methylesterification state and cell wall mechanical properties contribute to neighbor proximity‐induced hypocotyl growth in Arabidopsis","authors":"Fabien Sénéchal, Sarah Robinson, Evert Van Schaik, Martine Trévisan, Prashant Saxena, Didier Reinhardt, Christian Fankhauser","doi":"10.1002/pld3.584","DOIUrl":"https://doi.org/10.1002/pld3.584","url":null,"abstract":"Plants growing with neighbors compete for light and consequently increase the growth of their vegetative organs to enhance access to sunlight. This response, called shade avoidance syndrome (SAS), involves photoreceptors such as phytochromes as well as phytochrome interacting factors (PIFs), which regulate the expression of growth‐mediating genes. Numerous cell wall‐related genes belong to the putative targets of PIFs, and the importance of cell wall modifications for enabling growth was extensively shown in developmental models such as dark‐grown hypocotyl. However, the contribution of the cell wall in the growth of de‐etiolated seedlings regulated by shade cues remains poorly established. Through analyses of mechanical and biochemical properties of the cell wall coupled with transcriptomic analysis of cell wall‐related genes from previously published data, we provide evidence suggesting that cell wall modifications are important for neighbor proximity‐induced elongation. Further analysis using loss‐of‐function mutants impaired in the synthesis and remodeling of the main cell wall polymers corroborated this. We focused on the <jats:italic>cgr2cgr3</jats:italic> double mutant that is defective in methylesterification of homogalacturonan (HG)‐type pectins. By following hypocotyl growth kinetically and spatially and analyzing the mechanical and biochemical properties of cell walls, we found that methylesterification of HG‐type pectins was required to enable global cell wall modifications underlying neighbor proximity‐induced hypocotyl growth. Collectively, our work suggests that plant competition for light induces changes in the expression of numerous cell wall genes to enable modifications in biochemical and mechanical properties of cell walls that contribute to neighbor proximity‐induced growth.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"38 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140634427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Down-regulation of wheat Rubisco activase isoforms expression by virus-induced gene silencing","authors":"Juan Alejandro Perdomo, Joanna C. Scales, Wing-Sham Lee, Kostya Kanyuka, Elizabete Carmo-Silva","doi":"10.1002/pld3.583","DOIUrl":"https://doi.org/10.1002/pld3.583","url":null,"abstract":"Rubisco activase (Rca) is an essential photosynthetic enzyme that removes inhibitors from the catalytic sites of the carboxylating enzyme Rubisco. In wheat, Rca is composed of one longer 46 kDa α-isoform and two shorter 42 kDa β-isoforms encoded by the genes <i>TaRca1</i> and <i>TaRca2</i>. <i>TaRca1</i> produces a single transcript from which a short 1β-isoform is expressed, whereas two alternative transcripts are generated from <i>TaRca2</i> directing expression of either a long 2α-isoform or a short 2β-isoform. The 2β isoform is similar but not identical to 1β. Here, virus-induced gene silencing (VIGS) was used to silence the different <i>TaRca</i> transcripts. Abundance of the transcripts and the respective protein isoforms was then evaluated in the VIGS-treated and control plants. Remarkably, treatment with the construct specifically targeting <i>TaRca1</i> efficiently decreased expression not only of <i>TaRca1</i> but also of the two alternative <i>TaRca2</i> transcripts. Similarly, specific targeting of the <i>TaRca2</i> transcript encoding a long isoform <i>TaRca2α</i> resulted in silencing of both <i>TaRca2</i> alternative transcripts. The corresponding protein isoforms decreased in abundance. These findings indicate concomitant down-regulation of <i>TaRca1</i> and <i>TaRca2</i> at both transcript and protein levels and may impact the feasibility of altering the relative abundance of Rca isoforms in wheat.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"51 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140564929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of leaf extraction and storage conditions for eco‐metabolomics studies","authors":"Jakob Lang, Sergio E. Ramos, Marharyta Smohunova, Laurent Bigler, Meredith C. Schuman","doi":"10.1002/pld3.578","DOIUrl":"https://doi.org/10.1002/pld3.578","url":null,"abstract":"Mass spectrometry‐based plant metabolomics is frequently used to identify novel natural products or study the effect of specific treatments on a plant's metabolism. Reliable sample handling is required to avoid artifacts, which is why most protocols mandate shock freezing of plant tissue in liquid nitrogen and an uninterrupted cooling chain. However, the logistical challenges of this approach make it infeasible for many ecological studies. Especially for research in the tropics, permanent cooling poses a challenge, which is why many of those studies use dried leaf tissue instead. We screened a total of 10 extraction and storage approaches for plant metabolites extracted from maize leaf tissue across two cropping seasons to develop a methodology for agroecological studies in logistically challenging tropical locations. All methods were evaluated based on changes in the metabolite profile across a 2‐month storage period at different temperatures with the goal of reproducing the metabolite profile of the living plant as closely as possible. We show that our newly developed on‐site liquid–liquid extraction protocol provides a good compromise between sample replicability, extraction efficiency, material logistics, and metabolite profile stability. We further discuss alternative methods which showed promising results and feasibility of on‐site sample handling for field studies.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"196 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140565494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root hydraulic properties: An exploration of their variability across scales","authors":"Juan C. Baca Cabrera, Jan Vanderborght, Valentin Couvreur, Dominik Behrend, Thomas Gaiser, Thuy Huu Nguyen, Guillaume Lobet","doi":"10.1002/pld3.582","DOIUrl":"https://doi.org/10.1002/pld3.582","url":null,"abstract":"Root hydraulic properties are key physiological traits that determine the capacity of root systems to take up water, at a specific evaporative demand. They can strongly vary among species, cultivars or even within the same genotype, but a systematic analysis of their variation across plant functional types (PFTs) is still missing. Here, we reviewed published empirical studies on root hydraulic properties at the segment‐, individual root‐, or root system scale and determined its variability and the main factors contributing to it. This corresponded to a total of 241 published studies, comprising 213 species, including woody and herbaceous vegetation.We observed an extremely large range of variation (of orders of magnitude) in root hydraulic properties, but this was not caused by systematic differences among PFTs. Rather, the (combined) effect of factors such as root system age, driving force used for measurement, or stress treatments shaped the results. We found a significant decrease in root hydraulic properties under stress conditions (drought and aquaporin inhibition, <jats:italic>p</jats:italic> &lt; .001) and a significant effect of the driving force used for measurement (hydrostatic or osmotic gradients, <jats:italic>p</jats:italic> &lt; .001). Furthermore, whole root system conductance increased significantly with root system age across several crop species (<jats:italic>p</jats:italic> &lt; .01), causing very large variation in the data (&gt;2 orders of magnitude). Interestingly, this relationship showed an asymptotic shape, with a steep increase during the first days of growth and a flattening out at later stages of development. We confirmed this dynamic through simulations using a state‐of‐the‐art computational model of water flow in the root system for a variety of crop species, suggesting common patterns across studies and species.These findings provide better understanding of the main causes of root hydraulic properties variations observed across empirical studies. They also open the door to better representation of hydraulic processes across multiple plant functional types and at large scales. All data collected in our analysis has been aggregated into an open access database (<jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"https://roothydraulic-properties.shinyapps.io/database/\">https://roothydraulic-properties.shinyapps.io/database/</jats:ext-link>), fostering scientific exchange.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"107 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140564774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}