Plant Direct最新文献_第10页

Chitin and laminarin additively trigger wheat reactive oxygen species but not resistance to Fusarium head blight. 几丁质和昆布蛋白可额外触发小麦活性氧，但不能抵抗镰刀菌枯萎病。

IF 2.3 3区生物学

Plant Direct Pub Date : 2023-10-17 eCollection Date: 2023-10-01 DOI: 10.1002/pld3.538

Guixia Hao, Nicholas A Rhoades, Susan McCormick

引用次数: 0

Reducing nitrate and tobacco-specific nitrosamine level in burley tobacco leaves through grafting on flue-cured tobacco rootstock. 通过在烤烟砧木上嫁接降低白肋烟叶片中的硝酸盐和烟草特定亚硝胺水平。

IF 2.3 3区生物学

Plant Direct Pub Date : 2023-10-12 eCollection Date: 2023-10-01 DOI: 10.1002/pld3.536

Yuqing Feng, Yuanyuan Zhao, Geng Li, Hongzhi Shi

{"title":"Reducing nitrate and tobacco-specific nitrosamine level in burley tobacco leaves through grafting on flue-cured tobacco rootstock.","authors":"Yuqing Feng, Yuanyuan Zhao, Geng Li, Hongzhi Shi","doi":"10.1002/pld3.536","DOIUrl":"10.1002/pld3.536","url":null,"abstract":"Nitrosation of pyridine alkaloids in tobacco generates tobacco-specific nitrosamines (TSNAs), which are notable toxicants in tobacco products and smoke. Burley tobacco, a chloroplast- and nitrogen (N)-deficient phenotype that accumulates high levels of nitrate-nitrogen (NO3-N) in its leaves, is particularly susceptible to TSNAs formation. In this study, reciprocal pot and field grafting experiments were conducted using burley tobacco Eyan No.1 and flue-cured tobacco K326 to investigate whether grafting burley tobacco scions on flue-cured tobacco rootstocks could enhance pigment biosynthesis and photosynthesis, while reducing the NO3-N level in burley tobacco leaves. Grafting burley tobacco scions on flue-cured tobacco rootstocks significantly increased the total pigment content, photosynthetic rate, biomass, nitrate reductase and glutamine synthetase activities, as well as ammonium-nitrogen (NH4-N), total soluble and reducing sugar, and soluble protein levels in burley tobacco leaves compared with burley tobacco self-rooting, while decreasing the NO3-N level and nitrate-N to total N ratio. Transcriptomic analysis revealed that grafting resulted in upregulated expression of genes involved in starch, sucrose, porphyrin, chlorophyll, and N metabolism, as well as carbon fixation and carotenoid biosynthesis. The findings suggest that grafting on high N use efficiency rootstock is an exceptionally promising means of decreasing NO3-N accumulation by improving photosynthesis and N metabolism in the scion, thereby reducing the levels of harmful TSNAs.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/55/aa/PLD3-7-e536.PMC10568975.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41237959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myb-like transcription factors have epistatic effects on circadian clock function but additive effects on plant growth. Myb样转录因子对生物钟功能具有上位性影响，但对植物生长具有加性影响。

IF 2.3 3区生物学

Plant Direct Pub Date : 2023-10-06 eCollection Date: 2023-10-01 DOI: 10.1002/pld3.533

Cassandra L Hughes, Stacey L Harmer

{"title":"Myb-like transcription factors have epistatic effects on circadian clock function but additive effects on plant growth.","authors":"Cassandra L Hughes, Stacey L Harmer","doi":"10.1002/pld3.533","DOIUrl":"10.1002/pld3.533","url":null,"abstract":"The functions of closely related Myb-like repressor and Myb-like activator proteins within the plant circadian oscillator have been well-studied as separate groups, but the genetic interactions between them are less clear. We hypothesized that these repressors and activators would interact additively to regulate both circadian and growth phenotypes. We used CRISPR-Cas9 to generate new mutant alleles and performed physiological and molecular characterization of plant mutants for five of these core Myb-like clock factors compared with a repressor mutant and an activator mutant. We first examined circadian clock function in plants likely null for both the repressor proteins, CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY), and the activator proteins, REVEILLE 4 (RVE4), REVEILLE (RVE6), and REVEILLE (RVE8). The rve468 triple mutant has a long period and flowers late, while cca1 lhy rve468 quintuple mutants, similarly to cca1 lhy mutants, have poor circadian rhythms and flower early. This suggests that CCA1 and LHY are epistatic to RVE4, RVE6, and RVE8 for circadian clock and flowering time function. We next examined hypocotyl elongation and rosette leaf size in these mutants. The cca1 lhy rve468 mutants have growth phenotypes intermediate between cca1 lhy and rve468 mutants, suggesting that CCA1, LHY, RVE4, RVE6, and RVE8 interact additively to regulate growth. Together, our data suggest that these five Myb-like factors interact differently in regulation of the circadian clock versus growth. More generally, the near-norm al seedling phenotypes observed in the largely arrhythmic quintuple mutant demonstrate that circadian-regulated output processes, like control of hypocotyl elongation, do not always depend upon rhythmic oscillator function.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3c/2d/PLD3-7-e533.PMC10557472.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41128346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of genes associated with abiotic stress tolerance in sweetpotato using weighted gene co-expression network analysis. 利用加权基因共表达网络分析鉴定甘薯非生物胁迫耐受相关基因。

IF 2.3 3区生物学

Plant Direct Pub Date : 2023-10-03 eCollection Date: 2023-10-01 DOI: 10.1002/pld3.532

Mercy Kitavi, Dorcus C Gemenet, Joshua C Wood, John P Hamilton, Shan Wu, Zhangjun Fei, Awais Khan, C Robin Buell

{"title":"Identification of genes associated with abiotic stress tolerance in sweetpotato using weighted gene co-expression network analysis.","authors":"Mercy Kitavi, Dorcus C Gemenet, Joshua C Wood, John P Hamilton, Shan Wu, Zhangjun Fei, Awais Khan, C Robin Buell","doi":"10.1002/pld3.532","DOIUrl":"10.1002/pld3.532","url":null,"abstract":"Sweetpotato, Ipomoea batatas (L.), a key food security crop, is negatively impacted by heat, drought, and salinity stress. The orange-fleshed sweetpotato cultivar \"Beauregard\" was exposed to heat, salt, and drought treatments for 24 and 48 h to identify genes responding to each stress condition in leaves. Analysis revealed both common (35 up regulated, 259 down regulated genes in the three stress conditions) and unique sets of up regulated (1337 genes by drought, 516 genes by heat, and 97 genes by salt stress) and down regulated (2445 genes by drought, 678 genes by heat, and 204 genes by salt stress) differentially expressed genes (DEGs) suggesting common, yet stress-specific transcriptional responses to these three abiotic stressors. Gene Ontology analysis of down regulated DEGs common to both heat and salt stress revealed enrichment of terms associated with \"cell population proliferation\" suggestive of an impact on the cell cycle by the two stress conditions. To identify shared and unique gene co-expression networks under multiple abiotic stress conditions, weighted gene co-expression network analysis was performed using gene expression profiles from heat, salt, and drought stress treated 'Beauregard' leaves yielding 18 co-expression modules. One module was enriched for \"response to water deprivation,\" \"response to abscisic acid,\" and \"nitrate transport\" indicating synergetic crosstalk between nitrogen, water, and phytohormones with genes encoding osmotin, cell expansion, and cell wall modification proteins present as key hub genes in this drought-associated module. This research lays the groundwork for exploring to a further degree, mechanisms for abiotic stress tolerance in sweetpotato.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10546384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41107212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence. NAC结构域转录因子VNI2和ATAF2形成蛋白质复合物并调节叶片衰老。

IF 2.3 3区生物学

Plant Direct Pub Date : 2023-09-18 eCollection Date: 2023-09-01 DOI: 10.1002/pld3.529

Isura Sumeda Priyadarshana Nagahage, Kohei Matsuda, Kyoko Miyashita, Sumire Fujiwara, Chanaka Mannapperuma, Takuya Yamada, Shingo Sakamoto, Toshiki Ishikawa, Minoru Nagano, Misato Ohtani, Ko Kato, Hirofumi Uchimiya, Nobutaka Mitsuda, Maki Kawai-Yamada, Taku Demura, Masatoshi Yamaguchi

{"title":"NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence.","authors":"Isura Sumeda Priyadarshana Nagahage, Kohei Matsuda, Kyoko Miyashita, Sumire Fujiwara, Chanaka Mannapperuma, Takuya Yamada, Shingo Sakamoto, Toshiki Ishikawa, Minoru Nagano, Misato Ohtani, Ko Kato, Hirofumi Uchimiya, Nobutaka Mitsuda, Maki Kawai-Yamada, Taku Demura, Masatoshi Yamaguchi","doi":"10.1002/pld3.529","DOIUrl":"10.1002/pld3.529","url":null,"abstract":"The NAM, ATAF1/2, and CUC2 (NAC) domain transcription factor VND-INTERACTING2 (VNI2) negatively regulates xylem vessel formation by interacting with another NAC domain transcription factor, VASCULAR-RELATED NAC-DOMAIN7 (VND7), a master regulator of xylem vessel formation. Here, we screened interacting proteins with VNI2 using yeast two-hybrid assay and isolated two NAC domain transcription factors, Arabidopsis thaliana ACTIVATION FACTOR 2 (ATAF2) and NAC DOMAIN CONTAINING PROTEIN 102 (ANAC102). A transient gene expression assay showed that ATAF2 upregulates the expression of genes involved in leaf senescence, and VNI2 effectively inhibits the transcriptional activation activity of ATAF2. vni2 mutants accelerate leaf senescence, whereas ataf2 mutants delay leaf senescence. In addition, the accelerated leaf senescence phenotype of the vni2 mutant is recovered by simultaneous mutation of ATAF2. Our findings strongly suggest that VNI2 interacts with and inhibits ATAF2, resulting in negatively regulating leaf senescence.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10507225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41110397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blue-green fluorescence during hypersensitive cell death arises from phenylpropanoid deydrodimers. 超敏细胞死亡时蓝绿色荧光是由苯丙烷脱氢二聚体引起的。

IF 3 3区生物学

Plant Direct Pub Date : 2023-09-12 eCollection Date: 2023-09-01 DOI: 10.1002/pld3.531

Basem Kanawati, Marko Bertic, Franco Moritz, Felix Habermann, Ina Zimmer, David Mackey, Philippe Schmitt-Kopplin, Jörg-Peter Schnitzler, Jörg Durner, Frank Gaupels

{"title":"Blue-green fluorescence during hypersensitive cell death arises from phenylpropanoid deydrodimers.","authors":"Basem Kanawati, Marko Bertic, Franco Moritz, Felix Habermann, Ina Zimmer, David Mackey, Philippe Schmitt-Kopplin, Jörg-Peter Schnitzler, Jörg Durner, Frank Gaupels","doi":"10.1002/pld3.531","DOIUrl":"10.1002/pld3.531","url":null,"abstract":"Infection of Arabidopsis with avirulent Pseudomonas syringae and exposure to nitrogen dioxide (NO2) both trigger hypersensitive cell death (HCD) that is characterized by the emission of bright blue-green (BG) autofluorescence under UV illumination. The aim of our current work was to identify the BG fluorescent molecules and scrutinize their biosynthesis, localization, and functions during the HCD. Compared with wild-type (WT) plants, the phenylpropanoid-deficient mutant fah1 developed normal HCD except for the absence of BG fluorescence. Ultrahigh resolution metabolomics combined with mass difference network analysis revealed that WT but not fah1 plants rapidly accumulate dehydrodimers of sinapic acid, sinapoylmalate, 5-hydroxyferulic acid, and 5-hydroxyferuloylmalate during the HCD. FAH1-dependent BG fluorescence appeared exclusively within dying cells of the upper epidermis as detected by microscopy. Saponification released dehydrodimers from cell wall polymers of WT but not fah1 plants. Collectively, our data suggest that HCD induction leads to the formation of free BG fluorescent dehydrodimers from monomeric sinapates and 5-hydroxyferulates. The formed dehydrodimers move from upper epidermis cells into the apoplast where they esterify cell wall polymers. Possible functions of phenylpropanoid dehydrodimers are discussed.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/88/3f/PLD3-7-e531.PMC10496137.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10264543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous CAS9 editing of cpSRP43, LHCA6, and LHCA7 in Picochlorum celeri lowers chlorophyll levels and improves biomass productivity. CAS9 同时编辑 Picochlorum celeri 中的 cpSRP43、LHCA6 和 LHCA7 可降低叶绿素水平并提高生物量生产率。

IF 2.3 3区生物学

Plant Direct Pub Date : 2023-09-12 eCollection Date: 2023-09-01 DOI: 10.1002/pld3.530

Anagha Krishnan, Melissa Cano, Devin A Karns, Tyson A Burch, Maria Likhogrud, Moena Aqui, Shaun Bailey, John Verruto, William Lambert, Fedor Kuzminov, Mahva Naghipor, Yingjun Wang, Christopher C Ebmeier, Joseph C Weissman, Matthew C Posewitz

{"title":"Simultaneous CAS9 editing of cpSRP43, LHCA6, and LHCA7 in Picochlorum celeri lowers chlorophyll levels and improves biomass productivity.","authors":"Anagha Krishnan, Melissa Cano, Devin A Karns, Tyson A Burch, Maria Likhogrud, Moena Aqui, Shaun Bailey, John Verruto, William Lambert, Fedor Kuzminov, Mahva Naghipor, Yingjun Wang, Christopher C Ebmeier, Joseph C Weissman, Matthew C Posewitz","doi":"10.1002/pld3.530","DOIUrl":"10.1002/pld3.530","url":null,"abstract":"High cellular pigment levels in dense microalgal cultures contribute to excess light absorption. To improve photosynthetic yields in the marine microalga Picochlorum celeri, CAS9 gene editing was used to target the molecular chaperone cpSRP43. Depigmented strains (>50% lower chlorophyll) were generated, with proteomics showing attenuated levels of most light harvesting complex (LHC) proteins. Gene editing generated two types of cpSRP43 transformants with distinct lower pigment phenotypes: (i) a transformant (Δsrp43) with both cpSRP43 diploid alleles modified to encode non-functional polypeptides and (ii) a transformant (STR30309) with a 3 nt in-frame insertion in one allele at the CAS9 cut site (non-functional second allele), leading to expression of a modified cpSRP43. STR30309 has more chlorophyll than Δsrp43 but substantially less than wild type. To further decrease light absorption by photosystem I in STR30309, CAS9 editing was used to stack in disruptions of both LHCA6 and LHCA7 to generate STR30843, which has higher (5-24%) productivities relative to wild type in solar-simulating bioreactors. Maximal productivities required frequent partial harvests throughout the day. For STR30843, exemplary diel bioreactor yields of ~50 g m-2 day-1 were attained. Our results demonstrate diel productivity gains in P. celeri by lowering pigment levels.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/23/df/PLD3-7-e530.PMC10497401.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10260155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormonal and proteomic analyses of southern blight disease caused by Athelia rolfsii and root chitosan priming on Cannabis sativa in an in vitro hydroponic system. 在离体水培系统中对 Athelia rolfsii 和壳聚糖对大麻根部引诱引起的南方枯萎病进行激素和蛋白质组分析。

IF 2.3 3区生物学

Plant Direct Pub Date : 2023-09-08 eCollection Date: 2023-09-01 DOI: 10.1002/pld3.528

Pipob Suwanchaikasem, Shuai Nie, Jamie Selby-Pham, Robert Walker, Berin A Boughton, Alexander Idnurm

{"title":"Hormonal and proteomic analyses of southern blight disease caused by Athelia rolfsii and root chitosan priming on Cannabis sativa in an in vitro hydroponic system.","authors":"Pipob Suwanchaikasem, Shuai Nie, Jamie Selby-Pham, Robert Walker, Berin A Boughton, Alexander Idnurm","doi":"10.1002/pld3.528","DOIUrl":"10.1002/pld3.528","url":null,"abstract":"Southern blight disease, caused by the fungal pathogen Athelia rolfsii, suppresses plant growth and reduces product yield in Cannabis sativa agriculture. Mechanisms of pathology of this soil-borne disease remain poorly understood, with disease management strategies reliant upon broad-spectrum antifungal use. Exposure to chitosan, a natural elicitor, has been proposed as an alternative method to control diverse fungal diseases in an eco-friendly manner. In this study, C. sativa plants were grown in the Root-TRAPR system, a transparent hydroponic growth device, where plant roots were primed with .2% colloidal chitosan prior to A. rolfsii inoculation. Both chitosan-primed and unprimed inoculated plants displayed classical symptoms of wilting and yellowish leaves, indicating successful infection. Non-primed infected plants showed increased shoot defense responses with doubling of peroxidase and chitinase activities. The levels of growth and defense hormones including auxin, cytokinin, and jasmonic acid were increased 2-5-fold. In chitosan-primed infected plants, shoot peroxidase activity and phytohormone levels were decreased 1.5-4-fold relative to the unprimed infected plants. When compared with shoots, roots were less impacted by A. rolfsii infection, but the pathogen secreted cell wall-degrading enzymes into the root-growth solution. Chitosan priming inhibited root growth, with root lengths of chitosan-primed plants approximately 65% shorter than the control, but activated root defense responses, with root peroxidase activity increased 2.7-fold along with increased secretion of defense proteins. The results suggest that chitosan could be an alternative platform to manage southern blight disease in C. sativa cultivation; however, further optimization is required to maximize effectiveness of chitosan.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10275566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transformation and regeneration of DNA polymerase Θ mutant rice plants. DNA 聚合酶 Θ 突变水稻植株的转化和再生。

IF 2.3 3区生物学

Plant Direct Pub Date : 2023-09-05 eCollection Date: 2023-09-01 DOI: 10.1002/pld3.526

Ayako Nishizawa-Yokoi, Stanton B Gelvin

引用次数: 0

Effect of graphene on soybean root colonization by Bradyrhizobium strains. 石墨烯对巴西根瘤菌菌株在大豆根部定殖的影响。

IF 2.3 3区生物学

Plant Direct Pub Date : 2023-09-03 eCollection Date: 2023-09-01 DOI: 10.1002/pld3.522

Athira Sethu Madhavan, Lilia Ernestina Montanez Hernandez, Zheng Rong Gu, Senthil Subramanian

{"title":"Effect of graphene on soybean root colonization by Bradyrhizobium strains.","authors":"Athira Sethu Madhavan, Lilia Ernestina Montanez Hernandez, Zheng Rong Gu, Senthil Subramanian","doi":"10.1002/pld3.522","DOIUrl":"10.1002/pld3.522","url":null,"abstract":"Legume crops such as soybean obtain a large portion of their nitrogen nutrition through symbiotic nitrogen fixation by diazotrophic rhizobia bacteria in root nodules. However, nodule occupancy by low-capacity nitrogen-fixing rhizobia can lead to lower-than-optimal levels of nitrogen fixation. Seed/root coating with engineered materials such as graphene-carrying biomolecules that may promote specific attraction/attachment of desirable bacterial strains is a potential strategy that can help overcome this rhizobia competition problem. As a first step towards this goal, we assessed the impact of graphene on soybean and Bradyrhizobium using a set of growth, biochemical, and physiological assays. Three different concentrations of graphene were tested for toxicity in soybean (50, 250, and 1,000 mg/l) and Bradyrhizobia (25, 50, and 100 mg/l). Higher graphene concentrations (250 mg/l and 1,000 mg/l) promoted seed germination but slightly delayed plant development. Spectrometric and microscopy assays for hydrogen peroxide and superoxide anion suggested that specific concentrations of graphene led to higher levels of reactive oxygen species in the roots. In agreement, these roots also showed higher activities of antioxidant enzymes, catalase, and ascorbate peroxidase. Conversely, no toxic effects were detected on Bradyrhizobia treated with graphene, and neither did they have higher levels of reactive oxygen species. Graphene treatments at 250 mg/l and 1,000 mg/l significantly reduced the number of nodules, but rhizobia infection and the overall nitrogenase activity were not affected. Our results show that graphene can be used as a potential vehicle for seed/root treatment.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c8/0e/PLD3-7-e522.PMC10475502.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10166946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0