Plant Direct最新文献

{"title":"Characteristics of turion development in two aquatic carnivorous plants: Hormonal profiles, gas exchange and mineral nutrient content","authors":"L. Adamec, L. Plačková, Karel Doležal","doi":"10.1002/pld3.558","DOIUrl":"https://doi.org/10.1002/pld3.558","url":null,"abstract":"Abstract Turions are vegetative, dormant, and storage overwintering organs formed in perennial aquatic plants in response to unfavorable ecological conditions and originate by extreme condensation of apical shoot segments. The contents of cytokinins, auxins, and abscisic acid were estimated in shoot apices of summer growing, rootless aquatic carnivorous plants, Aldrovanda vesiculosa and Utricularia australis, and in developing turions at three stages and full maturity to reveal hormonal patterns responsible for turion development. The hormones were analyzed in miniature turion samples using ultraperformance liquid chromatography coupled with triple quadrupole mass spectrometry. Photosynthetic measurements in young leaves also confirmed relatively high photosynthetic rates at later turion stages. The content of active cytokinin forms was almost stable in A. vesiculosa during turion development but markedly decreased in U. australis . In both species, auxin content culminated in the middle of turion development and then decreased again. The content of abscisic acid as the main inhibitory hormone was very low in growing plants in both species but rose greatly at first developmental stages and stayed very high in mature turions. The hormonal data indicate a great strength of developing turions within sink–source relationships and confirm the central role of abscisic acid in regulating the turion development.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"84 21","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139458183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cadmium accumulation dynamics in the rice endosperm during grain filling revealed by autoradiography","authors":"A. Hirose, K. Tanoi, T. Nakanishi, N. Kobayashi","doi":"10.1002/pld3.562","DOIUrl":"https://doi.org/10.1002/pld3.562","url":null,"abstract":"Abstract Cadmium (Cd) is one of the environmental pollutants contaminated in our food. Several previous reports showed that rice polishing cannot be efficient to reduce Cd content in white rice, implying the characteristic Cd distribution in rice grain. However, Cd distribution has not been fully elucidated so far. Herein, 109Cd radiotracer experiment was performed using the rice seedlings at various time points after flowering to obtain autoradiographs of the brown rice to visually understand the Cd transport and distribution during the grain‐filling process. It was shown that 109Cd accumulated in the outermost area of the brown rice, and also in the middle part of the starchy endosperm, resulting in the appearance of the double circle distribution pattern, which was not observed in the autoradiographs of 65Zn. The inner circle of 109Cd located around the center of the endosperm was developed particularly at around 8 and 10 days after flowering. After this period, 109Cd started to deposit at the outer part of the endosperm, which was also found in the autoradiograph of 14C‐sucrose. Considering the physiology of grain development, the contribution of water transport and protein synthesis in the endosperm on the characteristic Cd distribution pattern was hypothesized.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"33 11","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139457618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STOP1-regulated SMALL AUXIN UP RNA55 (SAUR55) is involved in proton/malate co-secretion for Al tolerance in Arabidopsis","authors":"Raj Kishan Agrahari, Yuriko Kobayashi, Takuo Enomoto, Tasuku Miyachi, Marie Sakuma, Miki Fujita, Takuya Ogata, Yasunari Fujita, Satoshi Iuchi, Masatomo Kobayashi, Yoshiharu Y. Yamamoto, Hiroyuki Koyama","doi":"10.1002/pld3.557","DOIUrl":"https://doi.org/10.1002/pld3.557","url":null,"abstract":"Proton (H⁺) release is linked to aluminum (Al)-enhanced organic acids (OAs) excretion from the roots under Al rhizotoxicity in plants. It is well-reported that the Al-enhanced organic acid excretion mechanism is regulated by SENSITIVE TO PROTON RHIZOTOXICITY1 (STOP1), a zinc-finger TF that regulates major Al tolerance genes. However, the mechanism of H⁺ release linked to OAs excretion under Al stress has not been fully elucidated. Recent physiological and molecular-genetic studies have implicated the involvement of SMALL AUXIN UP RNAs (SAURs) in the activation of plasma membrane H⁺-ATPases for stress responses in plants. We hypothesized that STOP1 is involved in the regulation of Al-responsive <i>SAURs</i>, which may contribute to the co-secretion of protons and malate under Al stress conditions. In our transcriptome analysis of the roots of the <i>stop1</i> (sensitive to proton rhizotoxicity1) mutant, we found that STOP1 regulates the transcription of one of the <i>SAURs</i>, namely <i>SAUR55</i>. Furthermore, we observed that the expression of <i>SAUR55</i> was induced by Al and repressed in the STOP1 T-DNA insertion knockout (KO) mutant (<i>STOP1</i>-KO). Through in silico analysis, we identified a functional STOP1-binding site in the promoter of <i>SAUR55</i>. Subsequent in vitro and in vivo studies confirmed that STOP1 directly binds to the promoter of <i>SAUR55</i>. This suggests that STOP1 directly regulates the expression of <i>SAUR55</i> under Al stress. We next examined proton release in the rhizosphere and malate excretion in the T-DNA insertion KO mutant of SAUR55 (<i>saur55</i>), in conjunction with <i>STOP1</i>-KO. Both <i>saur55</i> and <i>STOP1</i>-KO suppressed rhizosphere acidification and malate release under Al stress. Additionally, the root growth of <i>saur55</i> was sensitive to Al-containing media. In contrast, the overexpressed line of <i>SAUR55</i> enhanced rhizosphere acidification and malate release, leading to increased Al tolerance. These associations with Al tolerance were also observed in natural variations of Arabidopsis. These findings demonstrate that transcriptional regulation of <i>SAUR55</i> by STOP1 positively regulates H⁺ excretion via PM H⁺-ATPase 2 which enhances Al tolerance by malate secretion from the roots of Arabidopsis. The activation of PM H⁺-ATPase 2 by SAUR55 was suggested to be due to PP2C.D2/D5 inhibition by interaction on the plasma membrane with its phosphatase. Furthermore, RNAi-suppression of <i>NtSTOP1</i> in tobacco shows suppression of rhizosphere acidification under Al stress, which was associated with the suppression of <i>SAUR55</i> orthologs, which are inducible by Al in tobacco. It suggests that transcriptional regulation of Al-inducible <i>SAUR</i>s by STOP1 plays a critical role in OAs excretion in several plant species as an Al tolerance mechanism.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"52 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139065201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PELOTA and HBS1 suppress co-translational messenger RNA decay in Arabidopsis.","authors":"Rong Guo, Brian D Gregory","doi":"10.1002/pld3.553","DOIUrl":"10.1002/pld3.553","url":null,"abstract":"<p><p>Various messenger RNA (mRNA) decay mechanisms play major roles in controlling mRNA quality and quantity in eukaryotic organisms under different conditions. While it is known that the recently discovered co-translational mRNA decay (CTRD), the mechanism that allows mRNAs to be degraded while still being actively translated, is prevalent in yeast, humans, and various angiosperms, the regulation of this decay mechanism is less well studied. Moreover, it is still unclear whether this decay mechanism plays any role in the regulation of specific physiological processes in eukaryotes. Here, by re-analyzing the publicly available polysome profiling or ribosome footprinting and degradome sequencing datasets, we discovered that highly translated mRNAs tend to have lower co-translational decay levels. Based on this finding, we then identified Pelota and Hbs1, the translation-related ribosome rescue factors, as suppressors of co-translational mRNA decay in Arabidopsis. Furthermore, we found that Pelota and Hbs1 null mutants have lower germination rates compared to the wild-type plants, implying that proper regulation of co-translational mRNA decay is essential for normal developmental processes. In total, our study provides further insights into the regulation of CTRD in Arabidopsis and demonstrates that this decay mechanism does play important roles in Arabidopsis physiological processes.</p>","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"7 12","pages":"e553"},"PeriodicalIF":3.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10751093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139040395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential expansion and retention patterns of LRR-RLK genes across plant evolution.","authors":"Zachary Kileeg, Aparna Haldar, Hasna Khan, Arooj Qamar, G Adam Mott","doi":"10.1002/pld3.556","DOIUrl":"10.1002/pld3.556","url":null,"abstract":"<p><p>To maximize overall fitness, plants must accurately respond to a host of growth, developmental, and environmental signals throughout their life. Many of these internal and external signals are perceived by the leucine-rich repeat receptor-like kinases, which play roles in regulating growth, development, and immunity. This largest family of receptor kinases in plants can be divided into subfamilies based on the conservation of the kinase domain, which demonstrates that shared evolutionary history often indicates shared molecular function. Here we investigate the evolutionary history of this family across the evolution of 112 plant species. We identify lineage-specific expansions of the malectin-domain containing subfamily LRR subfamily I primarily in the Brassicales and bryophytes. Most other plant lineages instead show a large expansion in LRR subfamily XII, which in Arabidopsis is known to contain key receptors in pathogen perception. This striking asymmetric expansion may reveal a dichotomy in the evolutionary history and adaptation strategies employed by plants. A greater understanding of the evolutionary pressures and adaptation strategies acting on members of this receptor family offers a way to improve functional predictions for orphan receptors and simplify the identification of novel stress-related receptors.</p>","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"7 12","pages":"e556"},"PeriodicalIF":2.3,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10739070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139032467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PhosBoost: Improved phosphorylation prediction recall using gradient boosting and protein language models.","authors":"Elly Poretsky, Carson M Andorf, Taner Z Sen","doi":"10.1002/pld3.554","DOIUrl":"https://doi.org/10.1002/pld3.554","url":null,"abstract":"<p><p>Protein phosphorylation is a dynamic and reversible post-translational modification that regulates a variety of essential biological processes. The regulatory role of phosphorylation in cellular signaling pathways, protein-protein interactions, and enzymatic activities has motivated extensive research efforts to understand its functional implications. Experimental protein phosphorylation data in plants remains limited to a few species, necessitating a scalable and accurate prediction method. Here, we present PhosBoost, a machine-learning approach that leverages protein language models and gradient-boosting trees to predict protein phosphorylation from experimentally derived data. Trained on data obtained from a comprehensive plant phosphorylation database, qPTMplants, we compared the performance of PhosBoost to existing protein phosphorylation prediction methods, PhosphoLingo and DeepPhos. For serine and threonine prediction, PhosBoost achieved higher recall than PhosphoLingo and DeepPhos (.78, .56, and .14, respectively) while maintaining a competitive area under the precision-recall curve (.54, .56, and .42, respectively). PhosphoLingo and DeepPhos failed to predict any tyrosine phosphorylation sites, while PhosBoost achieved a recall score of .6. Despite the precision-recall tradeoff, PhosBoost offers improved performance when recall is prioritized while consistently providing more confident probability scores. A sequence-based pairwise alignment step improved prediction results for all classifiers by effectively increasing the number of inferred positive phosphosites. We provide evidence to show that PhosBoost models are transferable across species and scalable for genome-wide protein phosphorylation predictions. PhosBoost is freely and publicly available on GitHub.</p>","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"7 12","pages":"e554"},"PeriodicalIF":3.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10732782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138831234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of temperature effects on the protein accumulation of the FT-FD module using newly generated Arabidopsis transgenic plants.","authors":"Kyung-Ho Park, Sol-Bi Kim, Jae-Hoon Jung","doi":"10.1002/pld3.552","DOIUrl":"10.1002/pld3.552","url":null,"abstract":"<p><p>Arabidopsis flowering is dependent on interactions between a component of the florigens FLOWERING LOCUS T (FT) and the basic leucine zipper (bZIP) transcription factor FD. These proteins form a complex that activates the genes required for flowering competence and integrates environmental cues, such as photoperiod and temperature. However, it remains largely unknown how FT and FD are regulated at the protein level. To address this, we created <i>FT</i> transgenic plants that express the N-terminal FLAG-tagged FT fusion protein under the control of its own promoter in <i>ft</i> mutant backgrounds. <i>FT</i> transgenic plants complemented the delayed flowering of the <i>ft</i> mutant and exhibited similar <i>FT</i> expression patterns to wild-type Col-0 plants in response to changes in photoperiod and temperature. Similarly, we generated <i>FD</i> transgenic plants in <i>fd</i> mutant backgrounds that express the N-terminal MYC-tagged FD fusion protein under the <i>FD</i> promoter, rescuing the late flowering phenotypes in the <i>fd</i> mutant. Using these transgenic plants, we investigated how temperature regulates the expression of FT and FD proteins. Temperature-dependent changes in FT and FD protein levels are primarily regulated at the transcript level, but protein-level temperature effects have also been observed to some extent. In addition, our examination of the expression patterns of FT and FD in different tissues revealed that similar to the spatial expression pattern of <i>FT</i>, <i>FD</i> mRNA was expressed in both the leaf and shoot apex, but FD protein was only detected in the apex, suggesting a regulatory mechanism that restricts FD protein expression in the leaf during the vegetative growth phase. These transgenic plants provided a valuable platform for investigating the role of the FT-FD module in flowering time regulation.</p>","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"7 12","pages":"e552"},"PeriodicalIF":2.3,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10727963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138809008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TurboID-mediated proximity labeling for screening interacting proteins of FIP37 in <i>Arabidopsis</i>.","authors":"Xiaofang Li, Yanping Wei, Qili Fei, Guilin Fu, Yu Gan, Chuanlin Shi","doi":"10.1002/pld3.555","DOIUrl":"https://doi.org/10.1002/pld3.555","url":null,"abstract":"<p><p>Proximity labeling was recently developed to detect protein-protein interactions and members of subcellular multiprotein structures in living cells. Proximity labeling is conducted by fusing an engineered enzyme with catalytic activity, such as biotin ligase, to a protein of interest (bait protein) to biotinylate adjacent proteins. The biotinylated protein can be purified by streptavidin beads, and identified by mass spectrometry (MS). TurboID is an engineered biotin ligase with high catalytic efficiency, which is used for proximity labeling. Although TurboID-based proximity labeling technology has been successfully established in mammals, its application in plant systems is limited. Here, we report the usage of TurboID for proximity labeling of FIP37, a core member of m⁶A methyltransferase complex, to identify FIP37 interacting proteins in <i>Arabidopsis thaliana</i>. By analyzing the MS data, we found 214 proteins biotinylated by GFP-TurboID-FIP37 fusion, including five components of m⁶A methyltransferase complex that have been previously confirmed. Therefore, the identified proteins may include potential proteins directly involved in the m⁶A pathway or functionally related to m⁶A-coupled mRNA processing due to spatial proximity. Moreover, we demonstrated the feasibility of proximity labeling technology in plant epitranscriptomics study, thereby expanding the application of this technology to more subjects of plant research.</p>","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"7 12","pages":"e555"},"PeriodicalIF":3.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10727772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138809011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"α-Tomatine gradient across artificial roots recreates the recruitment of tomato root-associated <i>Sphingobium</i>.","authors":"Kyoko Takamatsu, Miwako Toyofuku, Fuki Okutani, Shinichi Yamazaki, Masaru Nakayasu, Yuichi Aoki, Masaru Kobayashi, Kentaro Ifuku, Kazufumi Yazaki, Akifumi Sugiyama","doi":"10.1002/pld3.550","DOIUrl":"10.1002/pld3.550","url":null,"abstract":"<p><p>α-Tomatine is a major saponin that accumulates in tomatoes (<i>Solanum lycopersicum</i>). We previously reported that α-tomatine secreted from tomato roots modulates root-associated bacterial communities, particularly by enriching the abundance of <i>Sphingobium</i> belonging to the family <i>Sphingomonadaceae</i>. To further characterize the α-tomatine-mediated interactions between tomato plants and soil bacterial microbiota, we first cultivated tomato plants in pots containing different microbial inoculants originating from three field soils. Four bacterial genera, namely, <i>Sphingobium</i>, <i>Bradyrhizobium</i>, <i>Cupriavidus</i>, and <i>Rhizobacter</i>, were found to be commonly enriched in tomato root-associated bacterial communities. We constructed a pseudo-rhizosphere system using a mullite ceramic tube as an artificial root to investigate the influence of α-tomatine in modifying bacterial communities. The addition of α-tomatine from the artificial root resulted in the formation of a concentration gradient of α-tomatine that mimicked the tomato rhizosphere, and distinctive bacterial communities were observed in the soil close to the artificial root. <i>Sphingobium</i> was enriched according to the α-tomatine concentration gradient, whereas <i>Bradyrhizobium</i>, <i>Cupriavidus</i>, and <i>Rhizobacter</i> were not enriched in α-tomatine-treated soil. The tomato root-associated bacterial communities were similar to the soil bacterial communities in the vicinity of artificial root-secreting exudates; however, hierarchical cluster analysis revealed a distinction between root-associated and pseudo-rhizosphere bacterial communities. These results suggest that the pseudo-rhizosphere device at least partially creates a rhizosphere environment in which α-tomatine enhances the abundance of <i>Sphingobium</i> in the vicinity of the root. Enrichment of <i>Sphingobium</i> in the tomato rhizosphere was also apparent in publicly available microbiota data, further supporting the tight association between tomato roots and <i>Sphingobium</i> mediated by α-tomatine.</p>","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"7 12","pages":"e550"},"PeriodicalIF":2.3,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10728018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138809014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial community and chemical composition of cigar tobacco (Nicotiana tabacum L.) leaves altered by tobacco wildfire disease","authors":"Hongyang Si, Bing Cui, Fang Liu, Mingqin Zhao","doi":"10.1002/pld3.551","DOIUrl":"https://doi.org/10.1002/pld3.551","url":null,"abstract":"Tobacco wildfire disease caused by <i>Pseudomonas syringae</i> pv. <i>tabaci</i> is one of the most destructive foliar bacterial diseases occurring worldwide. However, the effect of wildfire disease on cigar tobacco leaves has not been clarified in detail. In this study, the differences in microbiota and chemical factors between wildfire disease-infected leaves and healthy leaves were characterized using high-throughput Illumina sequencing and a continuous-flow analytical system, respectively. The results demonstrated significant alterations in the structure of the phyllosphere microbial community in response to wildfire disease, and the infection of <i>P. syringae</i> pv. <i>tabaci</i> led to a decrease in bacterial richness and diversity. Furthermore, the content of nicotine, protein, total nitrogen, and Cl⁻ in diseased leaves significantly increased by 47.86%, 17.46%, 20.08%, and 72.77% in comparison to healthy leaves, while the levels of total sugar and reducing sugar decreased by 59.59% and 70.0%, respectively. Notably, the wildfire disease had little effect on the content of starch and K⁺. Redundancy analysis revealed that <i>Pseudomonas</i>, <i>Staphylococcus</i>, <i>Cladosporium</i>, and <i>Wallemia</i> displayed positive correlations with nicotine, protein, total nitrogen, Cl⁻ and K⁺ contents, while <i>Pantoea</i>, <i>Erwinia</i>, <i>Sphingomonas</i>, <i>Terrisporobacter</i>, <i>Aspergillus</i>, <i>Alternaria</i>, <i>Sampaiozyma</i>, and <i>Didymella</i> displayed positive correlations with total sugar and reducing sugar contents. <i>Brevibacterium</i>, <i>Brachybacterium</i>, and <i>Janibacter</i> were found to be enriched in diseased leaves, suggesting their potential role in disease suppression. Co-occurrence network analysis indicated that positive correlations were prevalent in microbial networks, and the bacterial network of healthy tobacco leaves exhibited greater complexity compared to diseased tobacco leaves. This study revealed the impact of wildfire disease on the microbial community and chemical compositions of tobacco leaves and provides new insights for the biological control of tobacco wildfire disease.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"39 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138628148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}