Plant Direct最新文献

{"title":"Project ChemicalBlooms: Collaborating with citizen scientists to survey the chemical diversity and phylogenetic distribution of plant epicuticular wax blooms","authors":"Le Thanh Dien Nguyen, Nicole Groth, Kylie Mondloch, Edgar B. Cahoon, Keith Jones, Lucas Busta","doi":"10.1002/pld3.588","DOIUrl":"https://doi.org/10.1002/pld3.588","url":null,"abstract":"Plants use chemistry to overcome diverse challenges. A particularly striking chemical trait that some plants possess is the ability to synthesize massive amounts of epicuticular wax that accumulates on the plant's surfaces as a white coating visible to the naked eye. The ability to synthesize basic wax molecules appears to be shared among virtually all land plants, and our knowledge of ubiquitous wax compound synthesis is reasonably advanced. However, the ability to synthesize thick layers of visible epicuticular crystals (“wax blooms”) is restricted to specific lineages, and our knowledge of how wax blooms differ from ubiquitous wax layers is less developed. Here, we recruited the help of citizen scientists and middle school students to survey the wax bloom chemistry of 78 species spanning dicot, monocot, and gymnosperm lineages. Using gas chromatography–mass spectrometry, we found that the major wax classes reported from bulk wax mixtures can be present in wax bloom crystals, with fatty acids, fatty alcohols, and alkanes being present in many species' bloom crystals. In contrast, other compounds including aldehydes, ketones, secondary alcohols, and triterpenoids were present in only a few species' wax bloom crystals. By mapping the 78 wax bloom chemical profiles onto a phylogeny and using phylogenetic comparative analyses, we found that secondary alcohol and triterpenoid‐rich wax blooms were present in lineage‐specific patterns that would not be expected to arise by chance. That finding is consistent with reports that secondary alcohol biosynthesis enzymes are found only in certain lineages but was a surprise for triterpenoids, which are intracellular components in virtually all plant lineages. Thus, our data suggest that a lineage‐specific mechanism other than biosynthesis exists that enables select species to generate triterpenoid‐rich surface wax crystals. Overall, our study outlines a general mode in which research scientists can collaborate with citizen scientists as well as middle and high school classrooms not only to enhance data collection and generate testable hypotheses but also to directly involve classrooms in the scientific process and inspire future STEM workers.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"15 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141063299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phaseolus vulgaris STP13.1 is an H+‐coupled monosaccharide transporter, present in source leaves and seed coats, with higher substrate affinity at depolarized potentials","authors":"Joseph L. Pegler, John W. Patrick, Benjamin McDermott, Anthony Brown, Jackson M. J. Oultram, Christopher P. L. Grof, John M. Ward","doi":"10.1002/pld3.585","DOIUrl":"https://doi.org/10.1002/pld3.585","url":null,"abstract":"Sugar transport proteins (STPs) are high‐affinity H<jats:sup>+</jats:sup>‐coupled hexose symporters. Recently, the contribution of STP13 to bacterial and fungal pathogen resistance across multiple plant species has garnered significant interest. Quantitative PCR analysis of source leaves, developing embryos, and seed coats of <jats:styled-content style=\"fixed-case\"><jats:italic>Phaseolus vulgaris</jats:italic></jats:styled-content> <jats:italic>L</jats:italic>. (common bean) revealed that <jats:italic>PvSTP13.1</jats:italic> was expressed in source leaves and seed coats throughout seed development. In contrast, <jats:italic>PvSTP13.1</jats:italic> transcripts were detected at exceedingly low levels in developing embryos. To characterize the transport mechanism, PvSTP13.1 was expressed in <jats:styled-content style=\"fixed-case\"><jats:italic>Xenopus laevis</jats:italic></jats:styled-content> oocytes, and inward‐directed currents were analyzed using two‐electrode voltage clamping. PvSTP13.1 was shown to function as an H<jats:sup>+</jats:sup>‐coupled monosaccharide symporter exhibiting a unique high affinity for hexoses and aldopentoses at depolarized membrane potentials. Specifically, of the 31 assessed substrates, which included aldohexoses, deoxyhexoses, fructose, 3‐O‐methyl‐D‐glucose, aldopentoses, polyols, glycosides, disaccharides, trisaccharides, and glucuronic acid, PvSTP13.1 displayed the highest affinity (<jats:italic>K</jats:italic><jats:sub>0.5</jats:sub>) for glucose (43 μM), mannose (92 μM), galactose (145 μM), fructose (224 μM), xylose (1.0 mM), and fucose (3.7 mM) at pH 5.6 at a depolarized membrane potential of −40 mV. The results presented here suggest PvSTP13.1 contributes to retrieval of hexoses from the apoplasmic space in source leaves and coats of developing seeds.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"52 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140634924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pectin methylesterification state and cell wall mechanical properties contribute to neighbor proximity‐induced hypocotyl growth in Arabidopsis","authors":"Fabien Sénéchal, Sarah Robinson, Evert Van Schaik, Martine Trévisan, Prashant Saxena, Didier Reinhardt, Christian Fankhauser","doi":"10.1002/pld3.584","DOIUrl":"https://doi.org/10.1002/pld3.584","url":null,"abstract":"Plants growing with neighbors compete for light and consequently increase the growth of their vegetative organs to enhance access to sunlight. This response, called shade avoidance syndrome (SAS), involves photoreceptors such as phytochromes as well as phytochrome interacting factors (PIFs), which regulate the expression of growth‐mediating genes. Numerous cell wall‐related genes belong to the putative targets of PIFs, and the importance of cell wall modifications for enabling growth was extensively shown in developmental models such as dark‐grown hypocotyl. However, the contribution of the cell wall in the growth of de‐etiolated seedlings regulated by shade cues remains poorly established. Through analyses of mechanical and biochemical properties of the cell wall coupled with transcriptomic analysis of cell wall‐related genes from previously published data, we provide evidence suggesting that cell wall modifications are important for neighbor proximity‐induced elongation. Further analysis using loss‐of‐function mutants impaired in the synthesis and remodeling of the main cell wall polymers corroborated this. We focused on the <jats:italic>cgr2cgr3</jats:italic> double mutant that is defective in methylesterification of homogalacturonan (HG)‐type pectins. By following hypocotyl growth kinetically and spatially and analyzing the mechanical and biochemical properties of cell walls, we found that methylesterification of HG‐type pectins was required to enable global cell wall modifications underlying neighbor proximity‐induced hypocotyl growth. Collectively, our work suggests that plant competition for light induces changes in the expression of numerous cell wall genes to enable modifications in biochemical and mechanical properties of cell walls that contribute to neighbor proximity‐induced growth.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"38 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140634427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Down-regulation of wheat Rubisco activase isoforms expression by virus-induced gene silencing","authors":"Juan Alejandro Perdomo, Joanna C. Scales, Wing-Sham Lee, Kostya Kanyuka, Elizabete Carmo-Silva","doi":"10.1002/pld3.583","DOIUrl":"https://doi.org/10.1002/pld3.583","url":null,"abstract":"Rubisco activase (Rca) is an essential photosynthetic enzyme that removes inhibitors from the catalytic sites of the carboxylating enzyme Rubisco. In wheat, Rca is composed of one longer 46 kDa α-isoform and two shorter 42 kDa β-isoforms encoded by the genes <i>TaRca1</i> and <i>TaRca2</i>. <i>TaRca1</i> produces a single transcript from which a short 1β-isoform is expressed, whereas two alternative transcripts are generated from <i>TaRca2</i> directing expression of either a long 2α-isoform or a short 2β-isoform. The 2β isoform is similar but not identical to 1β. Here, virus-induced gene silencing (VIGS) was used to silence the different <i>TaRca</i> transcripts. Abundance of the transcripts and the respective protein isoforms was then evaluated in the VIGS-treated and control plants. Remarkably, treatment with the construct specifically targeting <i>TaRca1</i> efficiently decreased expression not only of <i>TaRca1</i> but also of the two alternative <i>TaRca2</i> transcripts. Similarly, specific targeting of the <i>TaRca2</i> transcript encoding a long isoform <i>TaRca2α</i> resulted in silencing of both <i>TaRca2</i> alternative transcripts. The corresponding protein isoforms decreased in abundance. These findings indicate concomitant down-regulation of <i>TaRca1</i> and <i>TaRca2</i> at both transcript and protein levels and may impact the feasibility of altering the relative abundance of Rca isoforms in wheat.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"51 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140564929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of leaf extraction and storage conditions for eco‐metabolomics studies","authors":"Jakob Lang, Sergio E. Ramos, Marharyta Smohunova, Laurent Bigler, Meredith C. Schuman","doi":"10.1002/pld3.578","DOIUrl":"https://doi.org/10.1002/pld3.578","url":null,"abstract":"Mass spectrometry‐based plant metabolomics is frequently used to identify novel natural products or study the effect of specific treatments on a plant's metabolism. Reliable sample handling is required to avoid artifacts, which is why most protocols mandate shock freezing of plant tissue in liquid nitrogen and an uninterrupted cooling chain. However, the logistical challenges of this approach make it infeasible for many ecological studies. Especially for research in the tropics, permanent cooling poses a challenge, which is why many of those studies use dried leaf tissue instead. We screened a total of 10 extraction and storage approaches for plant metabolites extracted from maize leaf tissue across two cropping seasons to develop a methodology for agroecological studies in logistically challenging tropical locations. All methods were evaluated based on changes in the metabolite profile across a 2‐month storage period at different temperatures with the goal of reproducing the metabolite profile of the living plant as closely as possible. We show that our newly developed on‐site liquid–liquid extraction protocol provides a good compromise between sample replicability, extraction efficiency, material logistics, and metabolite profile stability. We further discuss alternative methods which showed promising results and feasibility of on‐site sample handling for field studies.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"196 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140565494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root hydraulic properties: An exploration of their variability across scales","authors":"Juan C. Baca Cabrera, Jan Vanderborght, Valentin Couvreur, Dominik Behrend, Thomas Gaiser, Thuy Huu Nguyen, Guillaume Lobet","doi":"10.1002/pld3.582","DOIUrl":"https://doi.org/10.1002/pld3.582","url":null,"abstract":"Root hydraulic properties are key physiological traits that determine the capacity of root systems to take up water, at a specific evaporative demand. They can strongly vary among species, cultivars or even within the same genotype, but a systematic analysis of their variation across plant functional types (PFTs) is still missing. Here, we reviewed published empirical studies on root hydraulic properties at the segment‐, individual root‐, or root system scale and determined its variability and the main factors contributing to it. This corresponded to a total of 241 published studies, comprising 213 species, including woody and herbaceous vegetation.We observed an extremely large range of variation (of orders of magnitude) in root hydraulic properties, but this was not caused by systematic differences among PFTs. Rather, the (combined) effect of factors such as root system age, driving force used for measurement, or stress treatments shaped the results. We found a significant decrease in root hydraulic properties under stress conditions (drought and aquaporin inhibition, <jats:italic>p</jats:italic> &lt; .001) and a significant effect of the driving force used for measurement (hydrostatic or osmotic gradients, <jats:italic>p</jats:italic> &lt; .001). Furthermore, whole root system conductance increased significantly with root system age across several crop species (<jats:italic>p</jats:italic> &lt; .01), causing very large variation in the data (&gt;2 orders of magnitude). Interestingly, this relationship showed an asymptotic shape, with a steep increase during the first days of growth and a flattening out at later stages of development. We confirmed this dynamic through simulations using a state‐of‐the‐art computational model of water flow in the root system for a variety of crop species, suggesting common patterns across studies and species.These findings provide better understanding of the main causes of root hydraulic properties variations observed across empirical studies. They also open the door to better representation of hydraulic processes across multiple plant functional types and at large scales. All data collected in our analysis has been aggregated into an open access database (<jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"https://roothydraulic-properties.shinyapps.io/database/\">https://roothydraulic-properties.shinyapps.io/database/</jats:ext-link>), fostering scientific exchange.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"107 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140564774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivated sunflower (Helianthus annuus L.) has lower tolerance of moderate drought stress than its con‐specific wild relative, but the underlying traits remain elusive","authors":"Vivian H. Tran, Kristen M. Nolting, Lisa A. Donovan, Andries A. Temme","doi":"10.1002/pld3.581","DOIUrl":"https://doi.org/10.1002/pld3.581","url":null,"abstract":"Cultivated crops are generally expected to have less abiotic stress tolerance than their wild relatives. However, this assumption is not well supported by empirical literature and may depend on the type of stress and how it is imposed, as well as the measure of tolerance being used. Here, we investigated whether wild and cultivated accessions of <jats:styled-content style=\"fixed-case\"><jats:italic>Helianthus annuus</jats:italic></jats:styled-content> differed in stress tolerance assessed as proportional decline in biomass due to drought and whether wild and cultivated accessions differed in trait responses to drought and trait associations with tolerance. In a greenhouse study, <jats:styled-content style=\"fixed-case\"><jats:italic>H. annuus</jats:italic></jats:styled-content> accessions in the two domestication classes (eight cultivated and eight wild accessions) received two treatments: a well‐watered control and a moderate drought implemented as a dry down followed by maintenance at a predetermined soil moisture level with automated irrigation. Treatments were imposed at the seedling stage, and plants were harvested after 2 weeks of treatment. The proportional biomass decline in response to drought was 24% for cultivated <jats:styled-content style=\"fixed-case\"><jats:italic>H. annuus</jats:italic></jats:styled-content> accessions but was not significant for the wild accessions. Thus, using the metric of proportional biomass decline, the cultivated accessions had less drought tolerance. Among accessions, there was no tradeoff between drought tolerance and vigor assessed as biomass in the control treatment. In a multivariate analysis, wild and cultivated accessions did not differ from each other or in response to drought for a subset of morphological, physiological, and allocational traits. Analyzed individually, traits varied in response to drought in wild and/or cultivated accessions, including declines in specific leaf area, leaf theoretical maximum stomatal conductance (g<jats:sub>smax</jats:sub>), and stomatal pore length, but there was no treatment response for stomatal density, succulence, or the ability to osmotically adjust. Focusing on traits associations with tolerance, plasticity in g<jats:sub>smax</jats:sub> was the most interesting because its association with tolerance differed by domestication class (although the effects were relatively weak) and thus might contribute to lower tolerance of cultivated sunflower. Our <jats:styled-content style=\"fixed-case\"><jats:italic>H. annuus</jats:italic></jats:styled-content> results support the expectation that stress tolerance is lower in crops than wild relatives under some conditions. However, determining the key traits that underpin differences in moderate drought tolerance between wild and cultivated <jats:styled-content style=\"fixed-case\"><jats:italic>H. annuus</jats:italic></jats:styled-content> remains elusive.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"1 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140565316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A K homology (KH) domain protein identified by a forward genetic screen affects bundle sheath anatomy in Arabidopsis thaliana","authors":"Zahida Bano, Peter Westhoff","doi":"10.1002/pld3.577","DOIUrl":"https://doi.org/10.1002/pld3.577","url":null,"abstract":"Because of their photosynthetic capacity, leaves function as solar panels providing the basis for the growth of the entire plant. Although the molecular mechanisms of leaf development have been well studied in model dicot and monocot species, a lot of information is still needed about the interplay of the genes that regulate cell division and differentiation and thereby affect the photosynthetic performance of the leaf. We were specifically interested in understanding the differentiation of mesophyll and bundle sheath cells in <jats:styled-content style=\"fixed-case\"><jats:italic>Arabidopsis thaliana</jats:italic></jats:styled-content> and aimed to identify genes that are involved in determining bundle sheath anatomy. To this end, we established a forward genetic screen by using ethyl methanesulfonate (EMS) for mutagenizing a reporter line expressing a chloroplast‐targeted green fluorescent protein (sGFP) under the control of a bundle sheath‐specific promoter. Based on the GFP fluorescence phenotype, numerous mutants were produced, and by pursuing a mapping‐by‐sequencing approach, the genomic segments containing mutated candidate genes were identified. One of the lines with an enhanced GFP fluorescence phenotype (named <jats:italic>ELEVATED BUNDLE SHEATH CELLS SIGNAL 1 [ebss1]</jats:italic>) was selected for further study, and the responsible gene was verified by CRISPR/Cas9‐based mutagenesis of candidate genes located in the mapped genomic segment. The verified gene, At2g25970, encodes a K homology (KH) domain‐containing protein.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"83 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140564754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A core of cell wall proteins functions in wall integrity responses in Arabidopsis thaliana","authors":"Oyeyemi Ajayi, Ellen Zelinsky, Charles T. Anderson","doi":"10.1002/pld3.579","DOIUrl":"https://doi.org/10.1002/pld3.579","url":null,"abstract":"Cell walls surround all plant cells, and their composition and structure are tightly regulated to maintain cellular and organismal homeostasis. In response to wall damage, the cell wall integrity (CWI) system is engaged to ameliorate effects on plant growth. Despite the central role CWI plays in plant development, our current understanding of how this system functions at the molecular level is limited. Here, we investigated the transcriptomes of etiolated seedlings of mutants of <jats:styled-content style=\"fixed-case\"><jats:italic>Arabidopsis thaliana</jats:italic></jats:styled-content> with defects in three major wall polysaccharides, pectin (<jats:italic>quasimodo2</jats:italic>), cellulose (<jats:italic>cellulose synthase3</jats:italic><jats:sup><jats:italic>je5</jats:italic></jats:sup>), and xyloglucan (<jats:italic>xyloglucan xylosyltransferase1</jats:italic> and <jats:italic>2</jats:italic>), to probe whether changes in the expression of cell wall‐related genes occur and are similar or different when specific wall components are reduced or missing. Many changes occurred in the transcriptomes of pectin‐ and cellulose‐deficient plants, but fewer changes occurred in the transcriptomes of xyloglucan‐deficient plants. We hypothesize that this might be because pectins interact with other wall components and/or integrity sensors, whereas cellulose forms a major load‐bearing component of the wall; defects in either appear to trigger the expression of structural proteins to maintain wall cohesion in the absence of a major polysaccharide. This core set of genes functioning in CWI in plants represents an attractive target for future genetic engineering of robust and resilient cell walls.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"6 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140564770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antagonistic functions of CTL1 and SUH1 mediate cell wall assembly in Arabidopsis","authors":"Nguyen Thi Thuy, Hyun‐Jung Kim, Suk‐Whan Hong","doi":"10.1002/pld3.580","DOIUrl":"https://doi.org/10.1002/pld3.580","url":null,"abstract":"Plant genomes contain numerous genes encoding chitinase‐like (CTL) proteins, which have a similar protein structure to chitinase belonging to the glycoside hydrolase (GH) family but lack the chitinolytic activity to cleave the <jats:italic>β</jats:italic>‐1,4‐glycosidic bond in chitins, polymers of <jats:italic>N</jats:italic>‐acetylglucosamine. <jats:italic>CTL1</jats:italic> mutations found in rice and <jats:italic>Arabidopsis</jats:italic> have caused pleiotropic developmental defects, including altered cell wall composition and decreased abiotic stress tolerance, likely due to reduced cellulose content. In this study, we identified <jats:italic>suppressor of hot2 1</jats:italic> (<jats:italic>suh1</jats:italic>) as a genetic suppressor of the <jats:italic>ctl1</jats:italic><jats:sup><jats:italic>hot2‐1</jats:italic></jats:sup> mutation in <jats:italic>Arabidopsis</jats:italic>. The mutation in <jats:italic>SUH1</jats:italic> restored almost all examined <jats:italic>ctl1</jats:italic><jats:sup><jats:italic>hot2‐1</jats:italic></jats:sup> defects to nearly wild‐type levels or at least partially. <jats:italic>SUH1</jats:italic> encodes a Golgi‐located type II membrane protein with glycosyltransferase (GT) activity, and its mutations lead to a reduction in cellulose content and hypersensitivity to cellulose biosynthesis inhibitors, although to a lesser extent than <jats:italic>ctl1</jats:italic><jats:sup><jats:italic>hot2‐1</jats:italic></jats:sup> mutation. The <jats:italic>SUH1</jats:italic> promoter fused with the GUS reporter gene exhibited GUS activity in interfascicular fibers and xylem in stems; meanwhile, the <jats:italic>ctl1</jats:italic><jats:sup><jats:italic>hot2‐1</jats:italic></jats:sup> mutation significantly increased this activity. Our findings provide genetic and molecular evidence that the antagonistic activities of CTL1 and SUH1 play an essential role in assembling the cell wall in <jats:italic>Arabidopsis</jats:italic>.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"304 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140199018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}