Plant Direct最新文献_第8页

Arabidopsis 3-Deoxy-d-Arabino-Heptulosonate 7-Phosphate (DAHP) Synthases of the Shikimate Pathway Display Both Manganese- and Cobalt-Dependent Activities. 莽草酸途径的3-脱氧-d-阿拉伯-七磷酸庚糖酸酯（DAHP）合成酶显示出锰和钴依赖活性。

IF 2.3 3区生物学

Plant Direct Pub Date : 2025-01-21 eCollection Date: 2025-01-01 DOI: 10.1002/pld3.70037

Ryo Yokoyama, Hiroshi A Maeda

{"title":"Arabidopsis 3-Deoxy-d-Arabino-Heptulosonate 7-Phosphate (DAHP) Synthases of the Shikimate Pathway Display Both Manganese- and Cobalt-Dependent Activities.","authors":"Ryo Yokoyama, Hiroshi A Maeda","doi":"10.1002/pld3.70037","DOIUrl":"10.1002/pld3.70037","url":null,"abstract":"The plant shikimate pathway directs a significant portion of photosynthetically assimilated carbon into the downstream biosynthetic pathways of aromatic amino acids (AAA) and aromatic natural products. 3-Deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthase (hereafter DHS) catalyzes the first step of the shikimate pathway, playing a critical role in controlling the carbon flux from central carbon metabolism into the AAA biosynthesis. Previous biochemical studies suggested the presence of manganese- and cobalt-dependent DHS enzymes (DHS-Mn and DHS-Co, respectively) in various plant species. Unlike well-studied DHS-Mn, however, the identity of DHS-Co is still unknown. Here, we show that all three DHS isoforms of Arabidopsis thaliana exhibit both DHS-Mn and DHS-Co activities in vitro. A phylogenetic analysis of various DHS orthologs and related sequences showed that Arabidopsis 3-deoxy-D-manno-octulosonate-8-phosphate synthase (KDOPS) proteins were closely related to microbial Type I DHSs. Despite their sequence similarity, these Arabidopsis KDOPS proteins showed no DHS activity. Meanwhile, optimization of the DHS assay conditions led to the successful detection of DHS-Co activity from Arabidopsis DHS recombinant proteins. Compared with DHS-Mn, DHS-Co activity displayed the same redox dependency but distinct optimal pH and cofactor sensitivity. Our work provides biochemical evidence that the DHS isoforms of Arabidopsis possess DHS-Co activity.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"9 1","pages":"e70037"},"PeriodicalIF":2.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-Wide Analysis of the APETALA2/Ethylene-Responsive Factor Gene Family in Carthamus tinctorius L. 红花APETALA2/乙烯反应因子基因家族的全基因组分析

IF 2.3 3区生物学

Plant Direct Pub Date : 2025-01-16 eCollection Date: 2025-01-01 DOI: 10.1002/pld3.70032

Zheng-Wei Tan, Dan-Dan Lu, Yong-Liang Yu, Lei Li, Lan-Jie Xu, Wei Dong, Chun-Ming Li, Qing Yang, Hui-Zhen Liang

{"title":"Genome-Wide Analysis of the APETALA2/Ethylene-Responsive Factor Gene Family in Carthamus tinctorius L.","authors":"Zheng-Wei Tan, Dan-Dan Lu, Yong-Liang Yu, Lei Li, Lan-Jie Xu, Wei Dong, Chun-Ming Li, Qing Yang, Hui-Zhen Liang","doi":"10.1002/pld3.70032","DOIUrl":"https://doi.org/10.1002/pld3.70032","url":null,"abstract":"The APETALA2/ethylene-responsive factor (AP2/ERF) superfamily represents a class of transcription factors involved in plant growth, development, and stress responses. Carthamus tinctorius L., also known as safflower, is an important plant whose flowers contain carthamin, an expensive aromatic pigment with various medicinal and flavoring properties. This study aimed to elucidate the roles of these transcription factors in plant growth, metabolic regulation, and environmental adaptation in safflower, providing foundational information and theoretical support for genetic improvement and stress resilience research in this crop. In this study, we identified and characterized the AP2/ERF family genes in safflower through a comprehensive genomic analysis. A total of 127 AP2/ERF genes were identified and clustered into seven groups and 14 subgroups based on phylogenetic analysis. Multiple sequence alignment revealed that the basic region and two helical structures were highly conserved in most AP2/ERF proteins. Cis-acting elements in the promoters of the AP2/ERF genes were analyzed, and a degree of safflower specificity was observed among different safflower species. Tissue-specific expression analysis showed that 23, 21, 15, and 9 genes were most abundantly expressed in the roots, leaves, flowers, and buds, respectively, while only eight genes were highly expressed in all tissues examined. These results indicate that the AP2/ERF family genes in safflower are diverse and complex, with distinct expression patterns for different genes in different safflower species. The findings provide important fundamental data for in-depth studies of the growth, development, and stress response mechanisms in safflower.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"9 1","pages":"e70032"},"PeriodicalIF":2.3,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chloroplast Vesiculation and Induced Chloroplast Vesiculation and Senescence-Associated Gene 12 Expression During Tomato Flower Pedicel Abscission. 番茄花梗脱落过程中叶绿体空泡和诱导的叶绿体空泡及衰老相关基因12的表达

IF 2.3 3区生物学

Plant Direct Pub Date : 2025-01-08 eCollection Date: 2025-01-01 DOI: 10.1002/pld3.70035

Magda Tušek Žnidarič, Maja Zagorščak, Živa Ramšak, Katja Stare, Marko Chersicola, Maruša Pompe Novak, Aleš Kladnik, Marina Dermastia

{"title":"Chloroplast Vesiculation and Induced Chloroplast Vesiculation and Senescence-Associated Gene 12 Expression During Tomato Flower Pedicel Abscission.","authors":"Magda Tušek Žnidarič, Maja Zagorščak, Živa Ramšak, Katja Stare, Marko Chersicola, Maruša Pompe Novak, Aleš Kladnik, Marina Dermastia","doi":"10.1002/pld3.70035","DOIUrl":"10.1002/pld3.70035","url":null,"abstract":"Abscission is a tightly regulated process in which plants shed unnecessary, infected, damaged, or aging organs, as well as ripe fruits, through predetermined abscission zones in response to developmental, hormonal, and environmental signals. Despite its importance, the underlying mechanisms remain incompletely understood. This study highlights the deleterious effects of abscission on chloroplast ultrastructure in the cells of the tomato flower pedicel abscission zone, revealing spatiotemporal differential gene expression and key transcriptional networks involved in chloroplast vesiculation during abscission. Significant changes in chloroplast structure and vesicle formation were observed 8 and 14 h after abscission induction, coinciding with the differential expression of vesiculation-related genes, particularly with upregulation of Senescence-Associated Gene 12 (SAG12) and Chloroplast Vesiculation (CV). This suggests a possible vesicle transport of chloroplast degrading material for recycling by autophagy-independent senescence-associated vacuoles (SAVs) and CV-containing vesicles (CCVs). Ethylene signaling appears to be involved in the regulation of these processes, as treatment with a competitive inhibitor of ethylene action, 1-methylcyclopropene, delayed vesiculation, reduced the expression of SAG12, and increased expression of Curvature Thylakoid 1A (CURT1A). In addition, chloroplast vesiculation during abscission was associated with differential expression of photosynthesis-related genes, particularly those involved in light reactions, underscoring the possible functional impact of the observed structural changes. This work provides new insights into the molecular and ultrastructural mechanisms underlying abscission and offers potential new targets for agricultural or biotechnological applications.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"9 1","pages":"e70035"},"PeriodicalIF":2.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11710935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modification of Gene Expression Involved in Alkaloid Production in Opium Poppy by VIGS Combined With Pretreatment of Macerozyme Enzyme. VIGS联合大酶酶预处理对罂粟生物碱生产相关基因表达的修饰

IF 2.3 3区生物学

Plant Direct Pub Date : 2025-01-07 eCollection Date: 2025-01-01 DOI: 10.1002/pld3.70034

Deniz Ece Özcan, Deniz Köm, Ozan Doğan, Semiha Erişen, Selma Onarici

{"title":"Modification of Gene Expression Involved in Alkaloid Production in Opium Poppy by VIGS Combined With Pretreatment of Macerozyme Enzyme.","authors":"Deniz Ece Özcan, Deniz Köm, Ozan Doğan, Semiha Erişen, Selma Onarici","doi":"10.1002/pld3.70034","DOIUrl":"10.1002/pld3.70034","url":null,"abstract":"Papaver somniferum L., a medicinal plant renowned for its pharmaceutical alkaloids, has captivated scientific interest due to its rich secondary metabolite profile. This study explores a novel approach to manipulating alkaloid biosynthesis pathways by integrating virus-induced gene silencing (VIGS) with macerozyme enzyme pretreatment. Targeting key genes in the benzylisoquinoline alkaloid (BIA) pathway (CODM, T6ODM, COR, DIOX2), the research aimed to elucidate the transformative potential of enzymatic preconditioning in somatic embryo cultures. To address the cell wall barrier, a known limitation in genetic manipulation, macerozyme pretreatment was employed, significantly enhancing gene silencing efficacy. Quantitative reverse transcription PCR analyses revealed significant alterations in gene expression profiles with macerozyme pretreatment, whereas no changes were observed in its absence. The T6ODM + DIOX combination was the most effective, reducing CODM, T6ODM, and DIOX2 expression by 72%, 65%, and 60%, respectively. Conversely, T6ODM expression increased by up to 107% in the CODM treatment. Notably, COR expression displayed dual regulatory dynamics, with suppression (47% decrease in T6ODM + DIOX) and enhancement (49% increase in CODM+DIOX) observed under different conditions. These findings underscore the complex interplay of gene regulation in the morphine biosynthesis pathway. This study highlights the critical role of macerozyme enzymatic pretreatment in overcoming cell wall barriers, enabling effective VIGS applications in somatic suspension cultures. The combination of VIGS and enzymatic pretreatment provides a robust platform for targeted metabolic engineering, offering insights into the regulation of morphine biosynthesis and paving the way for advancements in pharmaceutical alkaloid production and functional genomics in medicinal plants.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"9 1","pages":"e70034"},"PeriodicalIF":2.3,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11706800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomics of long-term, low oxygen storage coupled with ethylene signaling interference suggests neofunctionalization of hypoxia response pathways in apple (Malus domestica). 长期低氧储存和乙烯信号干扰的转录组学研究表明，苹果（Malus domestica）缺氧反应通路的新功能。

IF 2.3 3区生物学

Plant Direct Pub Date : 2024-12-20 eCollection Date: 2024-12-01 DOI: 10.1002/pld3.70025

John A Hadish, Heidi L Hargarten, Huiting Zhang, James P Mattheis, Stephen P Ficklin, Loren A Honaas

{"title":"Transcriptomics of long-term, low oxygen storage coupled with ethylene signaling interference suggests neofunctionalization of hypoxia response pathways in apple (Malus domestica).","authors":"John A Hadish, Heidi L Hargarten, Huiting Zhang, James P Mattheis, Stephen P Ficklin, Loren A Honaas","doi":"10.1002/pld3.70025","DOIUrl":"10.1002/pld3.70025","url":null,"abstract":"Research on how plants respond to hypoxia has concentrated on model organisms where tissues can only survive hypoxic conditions for a few hours to a few days. In contrast, hypoxic conditions are used commercially as a method to prolong the shelf life of Malus domestica (apple) fruit for up to a year of storage without substantial changes in fruit quality, not to mention a lack of tissue death. This ability of apples to withstand protracted hypoxic conditions is an interesting adaptation that has had limited molecular investigation despite its economic importance. Here, we investigate the long-term apple hypoxia response using a time-course RNA-seq analysis of several postharvest storage conditions. We use phylogenetics, differential expression, and regulatory networks to identify genes that regulate and are regulated by the hypoxia response. We identify potential neofunctionalization of core-hypoxia response genes in apples, including novel regulation of group VII ethylene response factor (ERF VII) and plant cysteine oxidase (PCO) family members.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"8 12","pages":"e70025"},"PeriodicalIF":2.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11660084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of β-Alanine From Isoleucine and Propionate Catabolism via Aminotransferases. 异亮氨酸和丙酸通过转氨酶分解代谢合成β-丙氨酸。

IF 2.3 3区生物学

Plant Direct Pub Date : 2024-12-18 eCollection Date: 2024-12-01 DOI: 10.1002/pld3.70030

Margo H Goldfarb, Joseph Boesel, Kai C Wilczewski-Shirai, Peter Reinhart, Trenton Scherger, Chloe Webb, Morgan Newlun, Kerry A Rouhier

{"title":"Synthesis of β-Alanine From Isoleucine and Propionate Catabolism via Aminotransferases.","authors":"Margo H Goldfarb, Joseph Boesel, Kai C Wilczewski-Shirai, Peter Reinhart, Trenton Scherger, Chloe Webb, Morgan Newlun, Kerry A Rouhier","doi":"10.1002/pld3.70030","DOIUrl":"10.1002/pld3.70030","url":null,"abstract":"In plants, the nonproteinogenic amino acid β-alanine plays a role in response to hypoxia, flooding, drought, heat, and heavy metal stress conditions. It is also a key intermediate in the synthesis of essential molecules including vitamin B5 and coenzyme A (CoA) through the condensation reaction with pantoate. While the syntheses of pantoate, vitamin B5, and CoA appear to be conserved across plants and bacteria, the synthesis of β-alanine is not. Bacteria and fungi use aspartate, whereas plants can use uracil, spermidine, or propionate to synthesize β-alanine. Given that these three precursors can be formed from the metabolism of glutamine, arginine, isoleucine, and valine, the synthesis of β-alanine could be linked to numerous pathways. Studies of valine catabolism in Arabidopsis suggested that some branched-chain amino acids could in fact serve as precursors for the synthesis of β-alanine. Using GC-MS and isotopically labeled isoleucine and propionate, we linked their metabolism to the synthesis of β-alanine via a proposed transamination of malonate semialdehyde. We then identified three aminotransferases that each catalyzed this final reversible transamination reaction. These results affirm our hypothesis that isoleucine metabolism is also linked to the synthesis of β-alanine via the transamination of metabolic intermediates.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"8 12","pages":"e70030"},"PeriodicalIF":2.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11655180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A bHLH Transcription Factor Confers Salinity Stress Tolerance in Betula platyphylla. 一种bHLH转录因子赋予白桦耐盐胁迫能力。

IF 2.3 3区生物学

Plant Direct Pub Date : 2024-12-17 eCollection Date: 2024-12-01 DOI: 10.1002/pld3.70029

Qilong Fang, Di Wu, Hu Sun, Luyao Wang, Yuping Liu, Wenfeng Mei, Huiyan Guo

{"title":"A bHLH Transcription Factor Confers Salinity Stress Tolerance in Betula platyphylla.","authors":"Qilong Fang, Di Wu, Hu Sun, Luyao Wang, Yuping Liu, Wenfeng Mei, Huiyan Guo","doi":"10.1002/pld3.70029","DOIUrl":"10.1002/pld3.70029","url":null,"abstract":"Basic helix-loop-helix (bHLH) proteins comprise a large family of transcription factors that are involved in plant growth and development, as well as responses to various types of environmental stress. Betula platyphylla (birch) is a pioneer tree species in secondary forest that plays a key role in maintaining ecosystem stability and forest regeneration, but few bHLHs involved in abiotic stress responses have been unveiled in birch. In this study, nine BpbHLH TFs related to stress responses in the birch genome were identified. Quantitative real-time polymerase chain reaction (RT-PCR) analysis indicated that the expression of these TFs can be induced by salt stress, and the expression of BpbHLH1 was higher than that of other BpbHLH genes. Particle bombardment analysis revealed that BpbHLH1 was localized to the nucleus. Yeast transformation found that BpbHLH1 has transcriptional activation activity. We generated BpbHLH1-overexpressing and silencing transgenic birch plants and subjected them to salt stress analysis. BpbHLH1 can enhance the salt tolerance of birch plants by increasing the reactive oxygen species scavenging ability and inhibiting cell death. Yeast one-hybrid, ß-glucuronidase, and chromatin immunoprecipitation assays revealed that BpbHLH1 can regulate the expression of target genes involved in stress resistance by binding to the E-box-1, E-box-2 and G-box elements in their promoters. The results of this study enhanced our understanding of the salt tolerance conferred by BpbHLH TFs in B. platyphylla and identified useful genes for the breeding of novel birch germplasm.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"8 12","pages":"e70029"},"PeriodicalIF":2.3,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11651711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined Physiological and Transcriptomic Analysis Reveals Key Regulatory Networks and Potential Hub Genes Controlling Chilling Tolerance During Soybean Germination. 结合生理和转录组学分析揭示了大豆发芽过程中关键的调控网络和潜在的枢纽基因。

IF 2.3 3区生物学

Plant Direct Pub Date : 2024-12-17 eCollection Date: 2024-12-01 DOI: 10.1002/pld3.70027

Jianguo Xie, Yuhong Zheng, Guang Li, Wei Zhang, Fanfan Meng, Xuhong Fan, Xingmiao Sun, Yunfeng Zhang, Mingliang Wang, Qingshan Chen, Shuming Wang, Hongwei Jiang

{"title":"Combined Physiological and Transcriptomic Analysis Reveals Key Regulatory Networks and Potential Hub Genes Controlling Chilling Tolerance During Soybean Germination.","authors":"Jianguo Xie, Yuhong Zheng, Guang Li, Wei Zhang, Fanfan Meng, Xuhong Fan, Xingmiao Sun, Yunfeng Zhang, Mingliang Wang, Qingshan Chen, Shuming Wang, Hongwei Jiang","doi":"10.1002/pld3.70027","DOIUrl":"10.1002/pld3.70027","url":null,"abstract":"Chilling is an important limiting factor for seed germination of soybean (Glycine max [L.] Merr.). To reveal the regulatory mechanism of chilling tolerance during the soybean germination stage, based on previous studies, the chilling tolerance line R48 and chilling sensitive line R89 in chromosome segment substitution lines were selected for physiological index determination and transcriptome sequencing. It was found that reactive oxygen species (ROS) scavenging system related enzymes, ROS, and osmotic regulators were significantly different between the two lines. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes enrichment were performed on the differentially expressed genes obtained by transcriptome sequencing. It was found that terms or pathways related to flavonoids, unsaturated fatty acids, and abscisic acid were highly enriched. In addition, weighted gene coexpression network analysis (WGCNA) method was used to analyze the physiological index data and transcriptome sequencing data. Four main coexpression modules significantly related to physiological indicators were obtained, and the hub genes in each module were screened according to eigengene-based connectivity value. Haplotype analysis of important candidate genes using soybean germplasm resources showed that there were significant differences in germination indexes between different major haplotypes of Glyma.17G163200. Based on the results of enrichment analysis and WGCNA, the regulation model of low temperature tolerance during soybean germination was preliminarily drawn. This study will provide theoretical guidance for analyzing the molecular regulation mechanism of cold tolerance in soybean germination stage.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"8 12","pages":"e70027"},"PeriodicalIF":2.3,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11651713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the role of grafting in abiotic stress management: Contemporary insights and automation trends. 探索嫁接在非生物胁迫管理中的作用：当代见解和自动化趋势。

IF 2.3 3区生物学

Plant Direct Pub Date : 2024-12-15 eCollection Date: 2024-12-01 DOI: 10.1002/pld3.70021

Kaukab Razi, Preethika Suresh, Pritam Paramguru Mahapatra, Musa Al Murad, Ajila Venkat, Michitaka Notaguchi, Dong Won Bae, Muthu Arjuna Samy Prakash, Sowbiya Muneer

{"title":"Exploring the role of grafting in abiotic stress management: Contemporary insights and automation trends.","authors":"Kaukab Razi, Preethika Suresh, Pritam Paramguru Mahapatra, Musa Al Murad, Ajila Venkat, Michitaka Notaguchi, Dong Won Bae, Muthu Arjuna Samy Prakash, Sowbiya Muneer","doi":"10.1002/pld3.70021","DOIUrl":"10.1002/pld3.70021","url":null,"abstract":"Grafting is a technique that involves attaching a rootstock to the aerial part of another genotype or species (scion), leading to improved crop performance and sustainable growth. The ability to tolerate abiotic stresses depends on cell membrane stability, a reduction in electrolyte leakage, and the species of scion and rootstock chosen. This external mechanism, grafting, serves as a beneficial tool in influencing crop performance by combining nutrient uptake and translocation to shoots, promoting sustainable plant growth, and enhancing the potential yield of both fruit and vegetable crops. Grafting helps to enhance crop production and improve the capacity of plants to utilize water when undergoing abiotic stress, particularly in genotypes that produce high yields upon rootstocks that are capable of decreasing the impact of drought stress on the shoot. The rootstock plays a pivotal role in establishing a grafted plant by forming a union between the graft and the rootstock. This process is characterized by its integrative, reciprocal nature, enabling plants to tolerate abiotic stress conditions. Grafting has been shown to alleviate the overproduction of lipid peroxidation and reactive oxygen species in the leaves and roots and enhance drought tolerance in plants by maintaining antioxidant enzyme activities and stress-responsive gene expression. Phytohormones, such as cytokinin, auxin, and gibberellin, play a critical role in maintaining rootstock-scion interactions. This review unveils the role of grafting in mitigating various environmental stressors, establishment of a robust graft junction, physiology of rootstock-scion communication, the mechanism underlying rootstock influence, hormonal regulations and the utilization of agri-bots in perfect healing and further cultivation of vegetable crops through grafting.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"8 12","pages":"e70021"},"PeriodicalIF":2.3,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11646695/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Arabidopsis Cell Culture With Weak Circadian Rhythms Under Constant Light Compared With Constant Dark Can Be Rescued by ELF3. 恒光条件下与恒暗条件下较弱昼夜节律的拟南芥细胞培养可通过ELF3进行拯救。

IF 2.3 3区生物学

Plant Direct Pub Date : 2024-11-28 eCollection Date: 2024-11-01 DOI: 10.1002/pld3.70028

Kanjana Laosuntisuk, Jigar S Desai, Colleen J Doherty

{"title":"An Arabidopsis Cell Culture With Weak Circadian Rhythms Under Constant Light Compared With Constant Dark Can Be Rescued by ELF3.","authors":"Kanjana Laosuntisuk, Jigar S Desai, Colleen J Doherty","doi":"10.1002/pld3.70028","DOIUrl":"10.1002/pld3.70028","url":null,"abstract":"Callus and cell suspension culture techniques are valuable tools in plant biotechnology and are widely used in fundamental and applied research. For studies in callus and cell suspension cultures to be relevant, it is essential to know if the underlying biochemistry is similar to intact plants. This study examined the expression of core circadian genes in Arabidopsis callus from the cell suspension named AT2 and found that the circadian rhythms were impaired. The circadian waveforms were like intact plants in the light/dark cycles, but the circadian expression in the AT2 callus became weaker in the free-running, constant light conditions. Temperature cycles could drive the rhythmic expression in constant conditions, but there were novel peaks at the point of temperature transitions unique to each clock gene. We found that callus freshly induced from seedlings had normal oscillations, like intact plants, suggesting that the loss of the circadian oscillation in the AT2 callus was specific to this callus. We determined that neither the media composition nor the source of the AT2 callus caused this disruption. We observed that ELF3 expression was not differentially expressed between dawn and dusk in both entrained, light-dark cycles and constant light conditions. Overexpression of AtELF3 in the AT2 callus partially recovers the circadian oscillation in the AT2 callus. This work shows that while callus and cell suspension cultures can be valuable tools for investigating plant responses, careful evaluation of their phenotype is important. Moreover, the altered circadian rhythms under constant light and temperature cycles in the AT2 callus could be useful backgrounds to understand the connections driving circadian oscillators and light and temperature sensing at the cellular level.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"8 11","pages":"1-16"},"PeriodicalIF":2.3,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11603095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0