Overexpression of CsBRC, an F-box gene from Camellia sinensis, increased the plant branching in tobacco and rice.

IF 2.3 3区生物学 Q2 PLANT SCIENCES

Plant Direct Pub Date : 2024-07-03 eCollection Date: 2024-07-01 DOI:10.1002/pld3.618

Bokun Zhou, Qi Sheng, Xinzhuan Yao, Tong Li, Litang Lu

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Abstract

Tea plant (Camellia sinensis [L.]) is one of the most important crops in China, and tea branch is an important agronomic trait that determines the yield of tea plant. In previous work focused on GWAS that detecting GWAS signals related to plant architecture through whole genome re-sequencing of ancient tea plants, a gene locus TEA 029928 significantly related to plant type was found. Sequence alignment results showed that this gene belonged to the F-box family. We named it CsBRC. CsBRC-GFP fusion proteins were mainly localized in the plasma membrane. By comparing the phenotypes of CsBRC transgenic tobacco and WT tobacco, it was found that the number of branches of transgenic tobacco was significantly higher than that of wild-type tobacco. Through RNA-seq analysis, it was found that CsBRC affects the branching development of plants by regulating the expression of genes related to brassinosteroid synthesis pathway in plants. In addition, overexpression of CsBRC in rice could increase tiller number, grain length and width, and 1,000-grain weight.

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过量表达来自山茶的 F-box 基因 CsBRC 增加了烟草和水稻的植株分枝。

茶树（Camellia sinensis [L.]）是中国最重要的农作物之一，而茶树分枝是决定茶树产量的重要农艺性状。前人的研究重点是通过对古茶树的全基因组重测序来检测与植物结构相关的GWAS信号，其中发现了一个与植物类型显著相关的基因位点TEA 029928。序列比对结果显示，该基因属于F-box家族。我们将其命名为 CsBRC。CsBRC-GFP融合蛋白主要定位于质膜。通过比较 CsBRC 转基因烟草和 WT 烟草的表型，发现转基因烟草的分枝数明显高于野生型烟草。通过 RNA-seq 分析发现，CsBRC 通过调控植物中铜绿素合成途径相关基因的表达来影响植物的分枝发育。此外，在水稻中过表达 CsBRC 能增加分蘖数、谷粒长度和宽度以及千粒重。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Direct Environmental Science-Ecology

CiteScore

5.00

自引率

3.30%

发文量

101

审稿时长

14 weeks

期刊介绍： Plant Direct is a monthly, sound science journal for the plant sciences that gives prompt and equal consideration to papers reporting work dealing with a variety of subjects. Topics include but are not limited to genetics, biochemistry, development, cell biology, biotic stress, abiotic stress, genomics, phenomics, bioinformatics, physiology, molecular biology, and evolution. A collaborative journal launched by the American Society of Plant Biologists, the Society for Experimental Biology and Wiley, Plant Direct publishes papers submitted directly to the journal as well as those referred from a select group of the societies’ journals.