Plant Direct最新文献_第4页

Genome and Tissue-Specific Transcriptome of the Tropical Milkweed (Asclepias curassavica). 热带乳草（Asclepias curassavica）基因组和组织特异性转录组。

IF 2.3 3区生物学

Plant Direct Pub Date : 2025-03-18 eCollection Date: 2025-03-01 DOI: 10.1002/pld3.70031

Honglin Feng, Jing Zhang, Adrian F Powell, Gretta L Buttelmann, Lily Yang, Ethan Yan, Fumin Wang, Steven B Broyles, Georg Jander, Susan R Strickler

{"title":"Genome and Tissue-Specific Transcriptome of the Tropical Milkweed (Asclepias curassavica).","authors":"Honglin Feng, Jing Zhang, Adrian F Powell, Gretta L Buttelmann, Lily Yang, Ethan Yan, Fumin Wang, Steven B Broyles, Georg Jander, Susan R Strickler","doi":"10.1002/pld3.70031","DOIUrl":"10.1002/pld3.70031","url":null,"abstract":"Tropical milkweed (Asclepias curassavica) serves as a host plant for monarch butterflies (Danaus plexippus) and other insect herbivores that can tolerate the abundant cardiac glycosides that are characteristic of this species. Cardiac glycosides, along with additional specialized metabolites, also contribute to the ethnobotanical uses of A. curassavica. To facilitate further research on milkweed metabolism, we assembled the 197-Mbp genome of a fifth-generation inbred line of A. curassavica into 619 contigs, with an N50 of 10 Mbp. Scaffolding resulted in 98% of the assembly being anchored to 11 chromosomes, which are mostly colinear with the previously assembled common milkweed (A. syriaca) genome. Assembly completeness evaluations showed that 98% of the BUSCO gene set is present in the A. curassavica genome assembly. The transcriptomes of six tissue types (young leaves, mature leaves, stems, flowers, buds, and roots), with and without defense elicitation by methyl jasmonate treatment, showed both tissue-specific gene expression and induced expression of genes that may be involved in cardiac glycoside biosynthesis. Expression of a CYP87A gene, the predicted first gene in the cardiac glycoside biosynthesis pathway, was observed only in the stems and roots and was induced by methyl jasmonate. Together, this genome sequence and transcriptome analysis provide important resources for further investigation of the ecological and medicinal uses of A. curassavica.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"9 3","pages":"e70031"},"PeriodicalIF":2.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11914377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of plasma membrane SUT1 in poplar alters lateral sucrose partitioning in stem and promotes leaf necrosis. 杨树质膜SUT1过表达改变茎侧蔗糖分配，促进叶片坏死。

IF 2.3 3区生物学

Plant Direct Pub Date : 2025-03-12 eCollection Date: 2025-03-01 DOI: 10.1002/pld3.70023

Liang-Jiao Xue, Moh'd I Hozain, Christopher J Frost, Afraz Talebi, Batbayar Nyamdari, Kavita B Aulakh, Ran Zhou, Scott A Harding, Chung-Jui Tsai

{"title":"Overexpression of plasma membrane SUT1 in poplar alters lateral sucrose partitioning in stem and promotes leaf necrosis.","authors":"Liang-Jiao Xue, Moh'd I Hozain, Christopher J Frost, Afraz Talebi, Batbayar Nyamdari, Kavita B Aulakh, Ran Zhou, Scott A Harding, Chung-Jui Tsai","doi":"10.1002/pld3.70023","DOIUrl":"10.1002/pld3.70023","url":null,"abstract":"In Populus and many other tree species, photoassimilate sucrose diffuses down a concentration gradient via symplastically connected mesophyll cells to minor vein phloem for long-distance transport. There is no evidence for apoplastic phloem-loading in Populus. However, plasma membrane sucrose transporters (SUT1 and SUT3) orthologous to those associated with apoplastic phloem loading are expressed in vascular tissues of poplar. While SUT3 functions in sucrose import into developing xylem, the role of SUT1 remains unclear. Here, we overexpressed PtaSUT1 in Populus tremula x P. alba to examine the effects on sucrose partitioning in transgenic plants. Overall leaf sucrose levels were similar between wild type and transgenic lines. Stem sucrose levels were not changed in bark but were significantly reduced in the adjacent xylem, suggesting hindered intercellular sucrose trafficking from the phloem to the developing xylem. Fully expanded leaves of transgenic plants deteriorated prematurely with declining photosynthesis prior to severe necrotic spotting. Necrotic spotting advanced most rapidly in the distal portion of mature leaves and was accompanied by sharp hexose increases and sharp sucrose decreases there. Leaf transcriptome profiling and network inference revealed the down-regulation of copper proteins and elevated expression of copper microRNAs prior to noticeable leaf injury. Our results suggest ectopic expression of PtaSUT1 altered sucrose partitioning in stems with systemic effects on leaf health and copper homeostasis mediated in part by sucrose-sensitive copper miRNAs.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"9 3","pages":"e70023"},"PeriodicalIF":2.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic Markers Associated With Soybean Resistance to the Stem Borer, Dectes texanus (Coleoptera: Cerambycidae). 大豆对茎螟虫（Dectes texanus）抗性的基因组标记。

IF 2.3 3区生物学

Plant Direct Pub Date : 2025-03-12 eCollection Date: 2025-03-01 DOI: 10.1002/pld3.70040

Sarah Johnson, David Hyten

{"title":"Genomic Markers Associated With Soybean Resistance to the Stem Borer, Dectes texanus (Coleoptera: Cerambycidae).","authors":"Sarah Johnson, David Hyten","doi":"10.1002/pld3.70040","DOIUrl":"10.1002/pld3.70040","url":null,"abstract":"The Dectes stem borer, Dectes texanus LeConte (Coleoptera: Cerambycidae), can significantly reduce yields by causing significant lodging in soybean. While this stem borer has not been considered a major pest of soybean, damage from it is increasing in the United States Midwest region with no current elite cultivars found resistant. Our objective was to map quantitative trait loci (QTL) that reduce girdled stems caused by Dectes stem borer infection and infestation of Dectes stem borer. A genome-wide association study (GWAS) using 50,000 single nucleotide polymorphisms was used to analyze data from a population of maturity group (MG) V to VII soybean accessions grown in North Carolina, which had been scored for Dectes stem borer larvae infestation and girdled stems caused by Dectes stem borer infestation. The GWAS identified 3 QTL with reduced larvae infestation and 4 QTL for reduced girdled stems. Allele effects ranged from 1% to 9% reduced larvae infestation or girdled stems. The QTL identified and germplasm containing the beneficial alleles can be used for improving resistance to the damage caused by the Dectes stem borer in elite soybean cultivars.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"9 3","pages":"e70040"},"PeriodicalIF":2.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11903490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppression of Chorismate Mutase 1 in Hybrid Poplar to Investigate Potential Redundancy in the Supply of Lignin Precursors. 抑制杂交杨木枝酸突变酶1研究木质素前体供应的潜在冗余。

IF 2.3 3区生物学

Plant Direct Pub Date : 2025-03-12 eCollection Date: 2025-03-01 DOI: 10.1002/pld3.70053

Yaseen Mottiar, Timothy J Tschaplinski, John Ralph, Shawn D Mansfield

{"title":"Suppression of Chorismate Mutase 1 in Hybrid Poplar to Investigate Potential Redundancy in the Supply of Lignin Precursors.","authors":"Yaseen Mottiar, Timothy J Tschaplinski, John Ralph, Shawn D Mansfield","doi":"10.1002/pld3.70053","DOIUrl":"10.1002/pld3.70053","url":null,"abstract":"Chorismate is an important branchpoint metabolite in the biosynthesis of lignin and a wide array of metabolites in plants. Chorismate mutase (CM), the enzyme responsible for transforming chorismate into prephenate, is a key regulator of metabolic flux towards the synthesis of aromatic amino acids and onwards to lignin. We examined three CM genes in hybrid poplar (Populus alba × grandidentata; P39, abbreviated as Pa×g) and used RNA interference (RNAi) to suppress the expression of Pa×gCM1, the most highly expressed isoform found in xylem tissue. Although this strategy was successful in disrupting Pa×gCM1 transcripts, there was also an unanticipated increase in lignin content, a shift towards guaiacyl lignin units, and more xylem vessels with smaller lumen areas, at least in the most severely affected transgenic line. This was accompanied by compensatory expression of the other two CM isoforms, Pa×gCM2 and Pa×gCM3, as well as widespread changes in gene expression and metabolism. This study investigates potential redundancy within the CM gene family in the developing xylem of poplar and highlights the pivotal role of chorismate in plant metabolism, development, and physiology.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"9 3","pages":"e70053"},"PeriodicalIF":2.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of Pleiotropic Regulatory Functions in Tannin1, the Sorghum Ortholog of Arabidopsis Master Regulator TTG1. 拟南芥主调控物TTG1的高粱同源物单宁1多效调控功能缺失

IF 2.3 3区生物学

Plant Direct Pub Date : 2025-03-12 eCollection Date: 2025-03-01 DOI: 10.1002/pld3.70055

Anthony Schuh, Geoffrey P Morris

{"title":"Loss of Pleiotropic Regulatory Functions in Tannin1, the Sorghum Ortholog of Arabidopsis Master Regulator TTG1.","authors":"Anthony Schuh, Geoffrey P Morris","doi":"10.1002/pld3.70055","DOIUrl":"10.1002/pld3.70055","url":null,"abstract":"Transcriptional master regulators are often targeted to improve plant traits, but antagonistic pleiotropic effects of these regulators can hamper this approach. The Myb-bHLH-WDR (MBW) complex is a broadly conserved transcriptional regulator affecting pigmentation, biotic stress resistance, and abiotic stress tolerance. We investigated the function of sorghum grain pigmentation regulator Tannin1, the ortholog of Arabidopsis pleiotropic WD40 regulator TTG1, to test for conserved pleiotropic regulatory effects and to better understand the evolution of the MBW complex in Poaceae. We characterized genome-wide differential expression of leaf tissue using RNA sequencing in near-isogenic lines (NILs) that contrasted wildtype Tan1 and loss-of-function tan1-b alleles, under optimal temperature and chilling stress. Notably, Gene Ontology analyses revealed no pathways with differential expression between Tan1 and tan1-b NILs, suggesting that, in contrast to Arabidopsis TTG1, Tannin1 has no pleiotropic regulatory role in leaves. Further, NILs had no visible difference in anthocyanin pigmentation, and no genes with known or expected function in flavonoid synthesis were differentially expressed. Genome-wide, only 18 total genes were differentially expressed between NILs, with six of these genes located inside the NIL introgression region, an observation most parsimoniously explained by cis-regulatory effects unrelated to Tannin1 regulation. Comparing our findings with known function of TTG1 orthologs in maize, rice, and Arabidopsis, we conclude that pleiotropic regulatory function in leaf tissue was likely lost in panicoid grass evolution before the sorghum-maize split. These findings inform future molecular breeding of MBW regulated traits and highlight the benefit of subfunctionalization to relieve pleiotropic constraints.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"9 3","pages":"e70055"},"PeriodicalIF":2.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11898007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ALPHA: A High Throughput System for Quantifying Growth in Aquatic Plants. ALPHA：水生植物生长定量的高通量系统。

IF 2.3 3区生物学

Plant Direct Pub Date : 2025-03-12 eCollection Date: 2025-03-01 DOI: 10.1002/pld3.70048

Kassidy A Robinson, Victoria Augoustides, Tanaka Madenyika, Ryan C Sartor

{"title":"ALPHA: A High Throughput System for Quantifying Growth in Aquatic Plants.","authors":"Kassidy A Robinson, Victoria Augoustides, Tanaka Madenyika, Ryan C Sartor","doi":"10.1002/pld3.70048","DOIUrl":"10.1002/pld3.70048","url":null,"abstract":"The need for more sustainable agricultural systems is becoming increasingly apparent. The global demand for agricultural products-food, feed, fuel and fiber-will continue to increase as the global population continues to grow. This challenge is compounded by climate change. Not only does a changing climate make it difficult to maintain stable yields but current agricultural systems are a major source of greenhouse gas emissions and continue to drive the problem further. Therefore, future agricultural systems must not only increase production but also significantly decrease negative environmental impacts. One approach to addressing this is to begin breeding and cultivating new plant species that have fundamental sustainability advantages over our existing crops. The Lemnaceae, commonly known as duckweeds, are one family of plants that have potential to increase output and reduce the negative environmental impacts of agricultural production. Herein we describe the Automated Lab-scale PHenotyping Apparatus, ALPHA, for high-throughput phenotyping of Lemnaceae. ALPHA is being used for selective breeding of one species, Lemna gibba, toward the goal of creating a new crop for use in sustainable agricultural systems. ALPHA can be used on many small aquatic plant species to assess growth rates in different environmental conditions. A proof of principle use case is demonstrated where ALPHA is used to determine saltwater tolerance of six different clones of L. gibba.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"9 3","pages":"e70048"},"PeriodicalIF":2.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unequal Genetic Redundancies Among MYC bHLH Transcription Factors Underlie Seedling Photomorphogenesis in Arabidopsis. MYC bHLH转录因子不平等遗传冗余是拟南芥幼苗光形态发生的基础。

IF 2.3 3区生物学

Plant Direct Pub Date : 2025-02-13 eCollection Date: 2025-02-01 DOI: 10.1002/pld3.70042

Vikas Garhwal, Sreya Das, Sreeramaiah N Gangappa

{"title":"Unequal Genetic Redundancies Among MYC bHLH Transcription Factors Underlie Seedling Photomorphogenesis in Arabidopsis.","authors":"Vikas Garhwal, Sreya Das, Sreeramaiah N Gangappa","doi":"10.1002/pld3.70042","DOIUrl":"10.1002/pld3.70042","url":null,"abstract":"Light is one of the most critical ecological cues controlling plant growth and development. Plants have evolved complex mechanisms to cope with fluctuating light signals. In Arabidopsis, bHLH transcription factors MYC2, MYC3, and MYC4 have been shown to play a vital role in protecting plants against herbivory and necrotrophic pathogens. While the role of MYC2 in light-mediated seedling development has been studied in some detail, the role of MYC3 and MYC4 still needs to be discovered. Here, we show that MYC4 negatively regulates seedling photomorphogenesis, while the MYC3 function seems redundant. However, the genetic analysis reveals that MYC3/MYC4 together act as positive regulators of seedling photomorphogenic growth as the myc3myc4 double mutants showed exaggerated hypocotyl growth compared to the myc3 and myc4 single mutants and Col-0. Intriguingly, the loss of MYC2 function in the myc3myc4 double mutant background (myc2myc3myc4) resulted in further enhancement in the hypocotyl growth than myc3myc4 double mutants in WL, BL and FRL, suggesting that MYC2/3/4 together play an essential and positive role in meditating optimal seedling photomorphogenesis. Besides, MYC3/MYC4 genetically and physically interact with HY5 to partially inhibit its function in controlling hypocotyl and photo-pigment accumulation. Moreover, our results suggest that COP1 physically interacts and degrades MYC3 and MYC4 through the 26S proteasomal pathway and controls their response to dark and light for fine-tuning HY5 function and seedling photomorphogenesis.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"9 2","pages":"e700042"},"PeriodicalIF":2.3,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11825187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LeafDNet: Transforming Leaf Disease Diagnosis Through Deep Transfer Learning. LeafDNet：通过深度迁移学习转化叶片疾病诊断。

IF 2.3 3区生物学

Plant Direct Pub Date : 2025-02-12 eCollection Date: 2025-02-01 DOI: 10.1002/pld3.70047

Tofayet Sultan, Mohammad Sayem Chowdhury, Nusrat Jahan, M F Mridha, Sultan Alfarhood, Mejdl Safran, Dunren Che

{"title":"LeafDNet: Transforming Leaf Disease Diagnosis Through Deep Transfer Learning.","authors":"Tofayet Sultan, Mohammad Sayem Chowdhury, Nusrat Jahan, M F Mridha, Sultan Alfarhood, Mejdl Safran, Dunren Che","doi":"10.1002/pld3.70047","DOIUrl":"10.1002/pld3.70047","url":null,"abstract":"The health and productivity of plants, particularly those in agricultural and horticultural industries, are significantly affected by timely and accurate disease detection. Traditional manual inspection methods are labor-intensive, subjective, and often inaccurate, failing to meet the precision required by modern agricultural practices. This research introduces an innovative deep transfer learning method utilizing an advanced version of the Xception architecture, specifically designed for identifying plant diseases in roses, mangoes, and tomatoes. The proposed model introduces additional convolutional layers following the base Xception architecture, combined with multiple trainable dense layers, incorporating advanced regularization and dropout techniques to optimize feature extraction and classification. This architectural enhancement enables the model to capture complex, subtle patterns within plant leaf images, contributing to more robust disease identification. A comprehensive dataset comprising 5491 images across four distinct disease categories was employed for the training, validation, and testing of the model. The experimental results showcased outstanding performance, achieving 98% accuracy, 99% precision, 98% recall, and a 98% F1-score. The model outperformed traditional techniques as well as other deep learning-based methods. These results emphasize the potential of this advanced deep learning framework as a scalable, efficient, and highly accurate solution for early plant disease detection, providing substantial benefits for plant health management and supporting sustainable agricultural practices.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"9 2","pages":"e70047"},"PeriodicalIF":2.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11815709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143409940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Promoter Collection for Cell-Targeted Analysis Within the Stomatal Complex. 气孔复合体细胞靶向分析的启动子集合。

IF 2.3 3区生物学

Plant Direct Pub Date : 2025-02-12 eCollection Date: 2025-02-01 DOI: 10.1002/pld3.70045

Thanh-Hao Nguyen, Jovaras Krasauskas, Thu Binh-Anh Nguyen, Azka Noureen, Mark Smedley, John M Christie, Wendy Harwood, Michael R Blatt, Penny Hundleby

{"title":"A Promoter Collection for Cell-Targeted Analysis Within the Stomatal Complex.","authors":"Thanh-Hao Nguyen, Jovaras Krasauskas, Thu Binh-Anh Nguyen, Azka Noureen, Mark Smedley, John M Christie, Wendy Harwood, Michael R Blatt, Penny Hundleby","doi":"10.1002/pld3.70045","DOIUrl":"10.1002/pld3.70045","url":null,"abstract":"Stomatal aperture is driven by changes in turgor of the guard cells that surround the stomatal pore. Epidermal cells immediately surrounding the guard cells are thought to contribute to the kinetics of aperture changes through changes in their turgor that opposes the guard cells and thought their putative roles in solute storage for use by the guard cells. Nonetheless, our knowledge remains fragmentary of surrounding cell mechanics, in large part because the tools and strategies needed to target the surrounding cells independent of the guard cells are limited. Here, we have analyzed sets of promoters for Arabidopsis, Brassica, and barley that will allow physiological studies of the roles of epidermal cells and also surrounding cells in the case of barley in stomatal behavior. These tissue-specific promoters offer distinct advantages over widely used, constitutive promoters by enabling precise and targeted gene expression within guard cells and the adjacent epidermal cells. As genetic tools, the promoters will have applications in strategies centered on physiological analyses and differential comparisons following expression targeted between the guard cells and the foliar epidermis as a whole. As such, they are well suited to questions around the mechanics of solute and water flux that will advance an understanding of the stomatal complex in these model species.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"9 2","pages":"e70045"},"PeriodicalIF":2.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11815712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143409886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Delivery of Marker-Free DNA to Plant Genome by the Transgenic Selection-Associated Fragment Elimination (T-SAFE) System. 利用转基因选择相关片段消除（T-SAFE）系统将无标记DNA传递到植物基因组。

IF 2.3 3区生物学

Plant Direct Pub Date : 2025-02-05 eCollection Date: 2025-02-01 DOI: 10.1002/pld3.70046

Yi Yang, Huan Chang, Leiwen Pan, Dongbei Guo, Shun Peng, Ting Mao, Yuehui Zhang, Shui Wang

{"title":"Delivery of Marker-Free DNA to Plant Genome by the Transgenic Selection-Associated Fragment Elimination (T-SAFE) System.","authors":"Yi Yang, Huan Chang, Leiwen Pan, Dongbei Guo, Shun Peng, Ting Mao, Yuehui Zhang, Shui Wang","doi":"10.1002/pld3.70046","DOIUrl":"10.1002/pld3.70046","url":null,"abstract":"The presence of a selection marker in transgenic plants has raised public concerns regarding health safety. We have developed a CRISPR/Cas9-based DNA delivery system termed transgenic selection-associated fragment elimination (T-SAFE). The T-SAFE system comprises four cassettes: the selection marker, CRISPR/Cas9, spacer-plus-protospacer adjacent motif (SP), and the cargo. The first two cassettes, the selection marker and CRISPR/Cas9, are collectively referred to as SCC. The SCC is flanked by two identical SPs derived from the fruit fly Ebony gene, which efficiently facilitate the SCC cleavage and subsequently lead to self-elimination of the selection marker upon integration of exogenous DNA into the plant genome. To inhibit the production of a functional Cas9 protein in bacteria, the IV2 intron of the potato ST-LS1 gene has been incorporated into the Cas9 gene. Additionally, the Cas9 gene is driven by a reproductive cell-specific or inducible promoter to avoid SCC cleavage in nonreproductive plant cells. These innovative features allow the T-SAFE system to achieve an elimination efficiency of the selection marker ranging from 10%-30% in Arabidopsis and 5%-8% in rice, with a DNA delivery capacity of approximately 10 kb. This approach offers a safe means for genetically modifying plants.","PeriodicalId":20230,"journal":{"name":"Plant Direct","volume":"9 2","pages":"e70046"},"PeriodicalIF":2.3,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11799591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0