Plant Biotechnology Reports最新文献_第2页

New dimension in leaf stomatal behavior analysis: a robust method with machine learning approach 叶气孔行为分析的新维度：采用机器学习方法的稳健方法

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-05-09 DOI: 10.1007/s11816-024-00902-8

Ki-Bon Ku, Anh Tuan Le, Thanh Tuan Thai, Sheikh Mansoor, Piya Kittipadakul, Janejira Duangjit, Ho-Min Kang, San Su Min Oh, Ngo Hoang Phan, Yong Suk Chung

{"title":"New dimension in leaf stomatal behavior analysis: a robust method with machine learning approach","authors":"Ki-Bon Ku, Anh Tuan Le, Thanh Tuan Thai, Sheikh Mansoor, Piya Kittipadakul, Janejira Duangjit, Ho-Min Kang, San Su Min Oh, Ngo Hoang Phan, Yong Suk Chung","doi":"10.1007/s11816-024-00902-8","DOIUrl":"https://doi.org/10.1007/s11816-024-00902-8","url":null,"abstract":"Stomata are specialized pores that play a vital role in gas exchange and photosynthesis. Microscopic images are often used to assess stomatal characteristics in plants; however, this can be a challenging task. By utilizing Matterport’s Mask R-CNN implementation as the foundational model, fine-tuning was conducted on a dataset of 810 microscopic images of Hedyotis corymbosa leaves’ surfaces for automated stomatal pores detection. The outcomes were promising, with the model achieving a convergence of 98% mean average precision (mAP) for both detection and segmentation. The training loss and validation loss values converged around 0.18 and 0.37, respectively. Regression analyses demonstrated the statistical significance (p values ≤ 0.05) of predictor parameters. Notably, the tightest cluster of data points was observed in stomata pore area measurements, followed by width and length. This highlights the precision of the stomatal pore area in characterizing stomatal traits. Despite challenges posed by the original dataset’s low-resolution images and artifacts like dust, bubbles, and blurriness, our innovative utilization of the Mask R-CNN algorithm yielded commendable outcomes. This research introduces a robust approach for stomatal phenotyping with broad applications in plant biology and environmental studies.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"2 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140930741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic, biochemical and genetic diversity of pepper (Capsicum spp.) germplasm reflects selection for cultivar types and spatial distribution 辣椒（辣椒属）种质的表型、生化和遗传多样性反映了对栽培品种类型和空间分布的选择

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-05-02 DOI: 10.1007/s11816-024-00904-6

Sayed Azrah Indrabi, Ajaz Malik, Geetika Malik, Khursheed Hussain, Asif Shikari, Javid Iqbal Mir, Ji Eun Park, Anh Tuan Le, Sheikh Mansoor, Khalid Z. Masoodi

{"title":"Phenotypic, biochemical and genetic diversity of pepper (Capsicum spp.) germplasm reflects selection for cultivar types and spatial distribution","authors":"Sayed Azrah Indrabi, Ajaz Malik, Geetika Malik, Khursheed Hussain, Asif Shikari, Javid Iqbal Mir, Ji Eun Park, Anh Tuan Le, Sheikh Mansoor, Khalid Z. Masoodi","doi":"10.1007/s11816-024-00904-6","DOIUrl":"https://doi.org/10.1007/s11816-024-00904-6","url":null,"abstract":"Throughout the globe morphological, biochemical and genetic variability exists in chilli and is harnessed to achieve specific breeding objectives. In this study, chilli germplasm was characterized based on horticultural traits, biochemical quantification and simple sequence repeat (SSR) polymorphism for diversity estimation. A total of 36 SSR primers were utilised to study the genetic divergence among 48 genotypes of chilli collected from nine states of India. Among the 36 primers, sixteen amplified null alleles. A total of 41 alleles were detected with average 2.05 alleles per locus. The largest number of alleles (5) were obtained with marker CAMS-234. The polymorphic information content ranged from 0.06 to 0.72 with an average of 0.50. On the basis of SSR analysis, the UPGMA cluster classified 48 genotypes into three groups. There was significant variability in germplasm for all morpho-biochemical traits. Kashi Anmol (100.50 q/ha) expressed the highest yield. Highest vitamin C content at green stage was recorded in IC-561635 (187 mg/100 g) and the greatest capsaicin content (9547.90 µg/g) equivalent to pungency of 171,862.2 Scoville heat units (SHU) was recorded in Bhut Jolokia. Principal component analysis indicates that the first five principal components explain 74.63% per cent of the total variation. Additionally, analysis of molecular variance (AMOVA) showed that 1% of the total genetic variation occurred among the population and 99% genetic variation within the populations, whereas the pairwise Fst specified the moderate genetic variation ranging from 0.002 to 0.020. The present investigation has strengthened the knowledge of genetic worth of this germplasm for application in various genetic improvement programmes.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"15 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140886972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influences of factors affecting the induction of high protoplast yield and callus induction in two different carnations 影响两种不同康乃馨原生质体产量和胼胝体诱导的因素的影响

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-04-29 DOI: 10.1007/s11816-024-00903-7

Oluwaseun Suleimon Adedeji, Aung Htay Naing, Jova Riza Campol, Kyeung Il Park, Chang Kil Kim

{"title":"Influences of factors affecting the induction of high protoplast yield and callus induction in two different carnations","authors":"Oluwaseun Suleimon Adedeji, Aung Htay Naing, Jova Riza Campol, Kyeung Il Park, Chang Kil Kim","doi":"10.1007/s11816-024-00903-7","DOIUrl":"https://doi.org/10.1007/s11816-024-00903-7","url":null,"abstract":"This study aims to establish an efficient protocol for protoplast isolation, cell division, and callus induction in two carnation cultivars, Chabaud and Giant Chabaud, by examining the influence of different types of plant tissue, enzyme concentrations, incubation times, cotyledon ages, and medium compositions. Our results indicate that protoplast yield varies significantly between different plant tissues, with true leaves offering the highest yield and viability, especially under a 0.1% driselase concentration and a 6-h incubation time. We observed that increasing the driselase concentration to 1.0% significantly reduced protoplast yields in all tissues tested. In addition, the age of the cotyledons notably affected protoplast yield, with younger cotyledons providing higher yields. The Murashige and Skoog medium supplemented with 1 mg/L zeatin and 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) proved to be the most effective for promoting cell division and colony formation from protoplasts derived from cotyledons and true leaves. The study also found that plant growth regulators (PGRs) significantly influence callus proliferation, with differences observed between protoplast sources.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"77 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140812768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of CRISPR/Cas9 ribonucleoprotein delivery into cabbage protoplasts for efficient DNA-free gene editing 优化 CRISPR/Cas9 核糖核蛋白向卷心菜原生质体的输送，实现高效的无 DNA 基因编辑

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-04-10 DOI: 10.1007/s11816-024-00901-9

Sora Lee, Su Hyun Park, Yu Jeong Jeong, Soyoung Kim, Bo Ryeong Kim, Bo-Keun Ha, Chaein Na, Jiyoung Lee, Jae Cheol Jeong, Cha Young Kim

{"title":"Optimization of CRISPR/Cas9 ribonucleoprotein delivery into cabbage protoplasts for efficient DNA-free gene editing","authors":"Sora Lee, Su Hyun Park, Yu Jeong Jeong, Soyoung Kim, Bo Ryeong Kim, Bo-Keun Ha, Chaein Na, Jiyoung Lee, Jae Cheol Jeong, Cha Young Kim","doi":"10.1007/s11816-024-00901-9","DOIUrl":"https://doi.org/10.1007/s11816-024-00901-9","url":null,"abstract":"The CRISPR/Cas9-based gene editing system for the direct delivery of pre-assembled Cas9 ribonucleoproteins (RNPs), consisting of a Cas9 nuclease and a single guide RNA (sgRNA), into plant protoplasts enables DNA-free gene editing without introducing foreign gene into plants. Here, we described the optimization of CRISPR/Cas9 RNPs delivery into cabbage protoplasts for efficient DNA-free gene editing. We determined the insertion and deletion (indel) frequency of BoMYBL2-1, a negative regulatory gene for anthocyanin biosynthesis in cabbage (Brassica oleracea var. capitata). We optimized the molar ratio of Cas9 to sgRNA and the incubation time of RNP–protoplast transfection to enhance the indel frequency under various conditions. Based on the BoMYBL2-1 nucleotide sequences, we designed nine sgRNAs to target BoMYBL2-1. Our in vitro digestion assay showed that all sgRNAs were able to cleave the targeted fragment. When the sgRNA and Cas9 proteins were subsequently transfected into protoplasts isolated from cabbage cotyledons, the deep sequencing results showed that the indel frequency of sgRNAs in BoMYBL2-1 was the highest (7.4%) with sgRNA3. We compared various molar ratios of Cas9 and sgRNA and incubation times of RNP–protoplast transfection to optimize transfection and ensure high indel frequency. The highest frequency was observed when the Cas9:sgRNA ratio was 1:10. Furthermore, when the incubation time for RNP–protoplast transfection was 1 min and 3 min, the indel frequency was higher than 25%. Altogether, these results provide valuable information on the optimized conditions for high-efficiency gene editing using CRISPR/Cas9 RNP delivery into cabbage protoplasts.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"148 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140588837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection and characterization of lead-tolerant sweetpotato cultivars for phytoremediation 用于植物修复的耐铅甘薯栽培品种的选育和特征描述

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-04-08 DOI: 10.1007/s11816-024-00900-w

Dias Daurov, Ye-Hoon Lim, Sul-U Park, Yun-Hee Kim, Ainash Daurova, Zagipa Sapakhova, Kuanysh Zhapar, Zhanar Abilda, Maxat Toishimanov, Malika Shamekova, Kabyl Zhambakin, Ho Soo Kim, Sang-Soo Kwak

{"title":"Selection and characterization of lead-tolerant sweetpotato cultivars for phytoremediation","authors":"Dias Daurov, Ye-Hoon Lim, Sul-U Park, Yun-Hee Kim, Ainash Daurova, Zagipa Sapakhova, Kuanysh Zhapar, Zhanar Abilda, Maxat Toishimanov, Malika Shamekova, Kabyl Zhambakin, Ho Soo Kim, Sang-Soo Kwak","doi":"10.1007/s11816-024-00900-w","DOIUrl":"https://doi.org/10.1007/s11816-024-00900-w","url":null,"abstract":"Lead (Pb) is one of the most toxic heavy metals (HMs) for plants and the environment. Sweetpotato [Ipomoea batatas (L.) Lam], the sixth most important food crop in the world, is tolerant to various environmental stresses, owing to its high antioxidant capacity. In this study, we selected sweetpotato cultivars showing high tolerance to lead (Pb) for phytoremediation-related applications. Young seedlings of 20 sweetpotato cultivars were treated with 30 mM Pb. Daeyumi (KO-12) and Dahomi (KO-5) were selected as Pb-tolerant and -sensitive cultivars, respectively, based on their photosynthetic activity and growth inhibition index (I50). In the Pb treatment, hydrogen peroxide and malondialdehyde contents of KO-12 were 1.5-fold less than those of KO-5. In addition, KO-12 showed a higher ability to accumulate Pb in roots and leaves than KO-5. Expression levels of four Pb-responsive genes, including the metallothionein gene IbMT1, were higher in the roots and leaves of KO-12 than in those of KO-5. Interestingly, KO-12 showed greater tolerance to high Pb concentrations than sunflower and rapeseed, which have been well-studied for phytoremediation. Our results suggest that sweetpotato is a suitable biomaterial for the phytoremediation of soils contaminated with HMs, including lead, for sustainable agriculture.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"50 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140589031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome analysis revealed that Arabidopsis model plant invokes the activation of heat shock proteins and ER stress response against cesium stress 转录组分析发现拟南芥模式植物在铯胁迫下会激活热休克蛋白和ER胁迫反应

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-04-04 DOI: 10.1007/s11816-024-00895-4

Dasom Choi, Dae Kwan Ko, Dong-Hwan Kim

{"title":"Transcriptome analysis revealed that Arabidopsis model plant invokes the activation of heat shock proteins and ER stress response against cesium stress","authors":"Dasom Choi, Dae Kwan Ko, Dong-Hwan Kim","doi":"10.1007/s11816-024-00895-4","DOIUrl":"https://doi.org/10.1007/s11816-024-00895-4","url":null,"abstract":"Cesium (Cs) toxicity has deleterious effects on plant growth and development. However, the molecular mechanism of the toxic effect of Cs on plants has been poorly understood. To obtain insights into the molecular events occurring in plants under Cs stress, we performed a comparative transcriptomic analysis between control and Cs-treated plants via RNA-seq. We identified 183 differentially expressed genes (141 upregulated and 42 downregulated) under Cs stress (1.5 mM CsCl). Gene ontology (GO) analysis using differentially expressed genes in Cs stress indicated that Cs triggered plant stress signaling pathways like reactive oxygen species (i.e., hydrogen peroxide). Further KEGG and MapMan metabolic pathway analyses revealed that many abiotic/biotic stress signaling pathways were highly induced. In particular, heat shock protein family genes were substantially induced upon exposure to Cs stress. We investigated the root growth of several knockout mutants of heat shock protein family genes and found that heat stress response was compromised in these mutants compared to wild type plants. It suggested that heat shock protein genes including HSP17s, HSP23s, HSP101, and HSFA2 proteins are deployed upon exposure to Cs for plant stress tolerance. Our study provided novel insights into the molecular events occurring in Cs-stressed plants.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"57 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140588725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of moss and Arabidopsis zinc-finger homeodomain transcription factors in regulating plant architecture 苔藓和拟南芥锌指同源转录因子在调控植物结构中的作用

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-04-01 DOI: 10.1007/s11816-024-00897-2

Young Koung Lee, Keunhwa Kim, Doreen Ware

{"title":"Role of moss and Arabidopsis zinc-finger homeodomain transcription factors in regulating plant architecture","authors":"Young Koung Lee, Keunhwa Kim, Doreen Ware","doi":"10.1007/s11816-024-00897-2","DOIUrl":"https://doi.org/10.1007/s11816-024-00897-2","url":null,"abstract":"Zinc-finger homeodomain transcription factors (ZF-HD TFs) are relatively a small gene family in Arabidopsis involved in plant development and stress response. However, the biological functions of ZF-HD TFs remain largely undiscovered. Here, we aimed to elucidate the evolutionary history and functional role of ZF-HD TFs in other species, by performing phylogenic analysis and domain and motif identification studies in Arabidopsis, sorghum (Sorghum bicolor), and moss (Physcomitrella patens). Forty-two ZF-HD TF proteins were classified into two distinct subfamilies based on the conserved ZF Cys/His-rich dimerization and homeodomain (HD) domains. The phylogenetic tree of proteins was further divided into five groups based on the similarity of sequences, and three distinct motifs were defined in the amino acid sequences. Genetic analysis revealed that the moss PpZF-HD1, Pp3c1_15290, gene partially rescued the amiR zf-HD-79 mutant lines at phenotypic and molecular levels. Subcellular localization studies revealed that moss PpZF-HD1 was localized in the cytosol and nuclei. Phylogenetic analysis and genetic complementation revealed that ZF-HD TFs play functional roles in regulating plant architecture, which is conserved in Arabidopsis, sorghum, and moss. Although our study is only a preliminary exploration into ZF-HD TFs, it provides a novel perspective that will help future researchers better understand the biological role of ZF-HD proteins in plants.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"23 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140588832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of an Agrobacterium-mediated genetic transformation and CRISPR/Cas9-mediated mutagenesis of haploid inducer genes in Pak-choi plants (Brassica rapa ssp. chinensis) 建立农杆菌介导的遗传转化和 CRISPR/Cas9 介导的白屈菜单倍体诱导基因诱变技术

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-03-26 DOI: 10.1007/s11816-024-00898-1

Young-Cheon Kim, May Phyo Thu, Falguni Maliha Rahman, Young Jae Yun, Jin Hoon Jang, Ok Ran Lee, Jeong Hwan Lee

{"title":"Establishment of an Agrobacterium-mediated genetic transformation and CRISPR/Cas9-mediated mutagenesis of haploid inducer genes in Pak-choi plants (Brassica rapa ssp. chinensis)","authors":"Young-Cheon Kim, May Phyo Thu, Falguni Maliha Rahman, Young Jae Yun, Jin Hoon Jang, Ok Ran Lee, Jeong Hwan Lee","doi":"10.1007/s11816-024-00898-1","DOIUrl":"https://doi.org/10.1007/s11816-024-00898-1","url":null,"abstract":"Pak-choi (Brassica rapa ssp. chinensis) is a popular vegetative crop in southern China, East Asia, and Southeast Asia. Owing to the threat of climate change, rapid breeding strategies for vegetable cultivars that are tolerant to abiotic and biotic stresses are required. Thus, the rapid fixation of useful agronomic traits using doubled haploid technology is urgent. The haploid-inducer gene is key to doubled haploidization. Two known CENH3 and pPLAIIγ genes, in which altered or partially deleted forms lead to haploid induction, were selected, and direct editing of Pak-choi CENH3 and pPLAIIγ genes (BcCENH3 and BcpPLAIIγ) was conducted using an Agrobacterium-mediated CRISPR/Cas9 system. First, BcCENH3 and BcpPLAIIγ genes were characterized by analyzing the spatial expression patterns and subcellular localization. The CENH3 expression levels in carpels and pPLAIIγ in various parts of Pak-choi flowers were higher than those of other parts. BcCENH3 and BcpPLAIIγ proteins targeted in the nucleus and plasma membrane, respectively. Whole plants were successfully regenerated from the shoot apical meristem (SAM) regions of Pak-choi seedlings using the optimized procedure and culture conditions. The regeneration results of SAM explants after Agrobacterium-mediated transformation of constructs expressing CRISPR/Cas9 and BcCENH3 or BcpPLAIIγ sgRNAs confirmed four independent BcCENH3-targeted transgenic lines with 2.1%, 1.8%, 1.8%, and 1.7% INDEL frequencies, and three independent BcpPLAIIγ-targeted transgenic lines with 24.5%, 33.7%, and 33.0% INDEL frequencies. Thus, our results suggested the possibility of developing transgenic Pak-choi lines by applying the CRISPR/Cas9 genome editing technology to BcCENH3 and BcpPLAIIγ as two haploid-inducer genes.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"44 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140315652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient CRISPR/Cas9-mediated gene editing of the ZjEIN2 gene in Zoysia japonica CRISPR/Cas9 介导的对车轴草 ZjEIN2 基因的高效基因编辑

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-02-29 DOI: 10.1007/s11816-024-00890-9

Jin Hee Kim, Mi-Young Park, Lanshuo Wang, Phan Phuong Thao Doan, Yueyue Yuan, Hyo-Yeon Lee, Jeongsik Kim

{"title":"Efficient CRISPR/Cas9-mediated gene editing of the ZjEIN2 gene in Zoysia japonica","authors":"Jin Hee Kim, Mi-Young Park, Lanshuo Wang, Phan Phuong Thao Doan, Yueyue Yuan, Hyo-Yeon Lee, Jeongsik Kim","doi":"10.1007/s11816-024-00890-9","DOIUrl":"https://doi.org/10.1007/s11816-024-00890-9","url":null,"abstract":"CRISPR/Cas9-based targeted gene editing has emerged as a versatile tool for deciphering gene function and improving traits in plants. However, this technique has not been applied to Zoysia japonica, a prominent warm-season turfgrass widely used for green spaces. Leaf senescence, a vital process affecting crop quality, occurs in Z. japonica during late growth, diminishing its aesthetic value and performance. In this study, we adeptly employed CRISPR/Cas9-mediated gene editing to create Z. japonica exhibiting delayed leaf senescence by targeting the ZjEIN2 gene, a crucial regulator of ethylene-mediated senescence. Precise gene editing, which generated knockout mutations in ZjEIN2, led to delayed leaf senescence in both dark and ethylene treatment conditions. This provided strong evidence for ZjEIN2’s role in leaf senescence regulation. These findings highlight the potential of CRISPR/Cas9-mediated gene editing as a biotechnological strategy to enhance anti-senescence traits in Z. japonica and potentially other crops. This study carries significant implications for sustainable agriculture and turfgrass management, offering promising avenues for future applications and research.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"20 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140010355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of physiological and biochemical activities of Eugenia uniflora by green-synthesized silver nanoparticle and melatonin under drought stress 干旱胁迫下绿色合成银纳米粒子和褪黑素对单叶洋金花生理生化活性的调节作用

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-02-21 DOI: 10.1007/s11816-024-00887-4

Ayomide H. Labulo, Oyinade A. David, Augustine D. Terna, Timileyin P. Omotosho, Nicholas S. Tanko, Ibrahim Hassan, Bosede R. Oluwole, Adeyinka Odebode

{"title":"Modulation of physiological and biochemical activities of Eugenia uniflora by green-synthesized silver nanoparticle and melatonin under drought stress","authors":"Ayomide H. Labulo, Oyinade A. David, Augustine D. Terna, Timileyin P. Omotosho, Nicholas S. Tanko, Ibrahim Hassan, Bosede R. Oluwole, Adeyinka Odebode","doi":"10.1007/s11816-024-00887-4","DOIUrl":"https://doi.org/10.1007/s11816-024-00887-4","url":null,"abstract":"The development of an effective and eco-friendly silver nanoparticle (AgNPs) to abate the effect of abiotic stress is an important area of nano-biotechnology. This study aimed to study the priming effect of plant-based green-synthesized silver nanoparticles and melatonin on the physiological and biochemical activities of drought-stressed E. uniflora. Sterilized seeds of E. uniflora were primed with 0.06 mg/l of ML-AgNPs, 0.06 mg/l of melatonin, and a nano-silver formulation of melatonin (1:1). Primed seeds were planted and subjected to 7 days under drought stress. The ML-AgNPs enhanced germination percentage, speed and vigor, and shoot elongation and induced the production of APx, CAT, and proline dehydrogenase (100% increases). Melatonin improved the activities of APx and CAT, total protein, accumulation of proline, and proline dehydrogenase (200% increases) and stabilized MDA content. Meanwhile, silver nano-formulation of melatonin increased leaves proliferation of leaves and production of APx, GPx, SOD, and CAT. Accumulation of proline and 100% upregulation of proline dehydrogenase osmo-regulated the effects of the drought, reduced MDA contents, and stabilized the excessive production of H2O2 and O2−. The ML-AgNO3 showed an efficient delivery system of melatonin into the plant under drought stress. As a result, our research shows that melatonin in silver nano-formulation (1:1) is a useful biostimulant against drought stress.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"18 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139921451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0