Green biotherapeutics: overcoming challenges in plant-based expression platforms

IF 1.7 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Plant Biotechnology Reports Pub Date : 2024-06-21 DOI:10.1007/s11816-024-00910-8

Rishabh Rajkumar Jadhav, Deepa Khare

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Abstract

Plant-based expression platforms offer a promising avenue to produce biotherapeutics due to their scalability, cost-effectiveness, and potential for complex protein expression. However, the widespread adoption of these platforms faces significant challenges. This review provides an overview of the current landscape of plant-based expression systems, highlighting their advantages and limitations. Regulatory hurdles, concerns about product purity and consistency, technical limitations, and economic considerations are discussed as major obstacles to the utilization of plant-based platforms. Strategies for overcoming these challenges, including advancements in genetic engineering, optimization of protein expression, post-translational modification and quality, and implementation of stringent quality control measures, are explored. Case studies and success stories illustrate the feasibility and potential of plant-based expression platforms for commercial production. Future perspectives and opportunities for collaboration between academia, industry, and regulatory agencies are also discussed. Ultimately, this review aims to provide insights into the potential of plant-based expression platforms and the path forward to harness their full potential in the production of green biotherapeutics.

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绿色生物疗法：克服基于植物的表达平台所面临的挑战

基于植物的表达平台因其可扩展性、成本效益和表达复杂蛋白质的潜力，为生产生物治疗药物提供了一条前景广阔的途径。然而，这些平台的广泛应用面临着巨大的挑战。本综述概述了基于植物的表达系统的现状，重点介绍了它们的优势和局限性。其中讨论了监管障碍、对产品纯度和一致性的担忧、技术限制和经济因素，这些都是利用基于植物的平台的主要障碍。探讨了克服这些挑战的策略，包括基因工程的进步、蛋白质表达的优化、翻译后修饰和质量，以及实施严格的质量控制措施。案例研究和成功案例说明了基于植物的表达平台用于商业生产的可行性和潜力。此外，还讨论了学术界、工业界和监管机构之间未来的合作前景和机会。最后，本综述旨在深入探讨基于植物的表达平台的潜力，以及在生产绿色生物治疗药物过程中充分发挥其潜力的前进道路。

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来源期刊

Plant Biotechnology Reports 生物-生物工程与应用微生物

CiteScore

4.10

自引率

4.20%

发文量

审稿时长

>12 weeks

期刊介绍： Plant Biotechnology Reports publishes original, peer-reviewed articles dealing with all aspects of fundamental and applied research in the field of plant biotechnology, which includes molecular biology, genetics, biochemistry, cell and tissue culture, production of secondary metabolites, metabolic engineering, genomics, proteomics, and metabolomics. Plant Biotechnology Reports emphasizes studies on plants indigenous to the Asia-Pacific region and studies related to commercialization of plant biotechnology. Plant Biotechnology Reports does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.