Influences of factors affecting the induction of high protoplast yield and callus induction in two different carnations

IF 1.7 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Oluwaseun Suleimon Adedeji, Aung Htay Naing, Jova Riza Campol, Kyeung Il Park, Chang Kil Kim
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Abstract

This study aims to establish an efficient protocol for protoplast isolation, cell division, and callus induction in two carnation cultivars, Chabaud and Giant Chabaud, by examining the influence of different types of plant tissue, enzyme concentrations, incubation times, cotyledon ages, and medium compositions. Our results indicate that protoplast yield varies significantly between different plant tissues, with true leaves offering the highest yield and viability, especially under a 0.1% driselase concentration and a 6-h incubation time. We observed that increasing the driselase concentration to 1.0% significantly reduced protoplast yields in all tissues tested. In addition, the age of the cotyledons notably affected protoplast yield, with younger cotyledons providing higher yields. The Murashige and Skoog medium supplemented with 1 mg/L zeatin and 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) proved to be the most effective for promoting cell division and colony formation from protoplasts derived from cotyledons and true leaves. The study also found that plant growth regulators (PGRs) significantly influence callus proliferation, with differences observed between protoplast sources.

Abstract Image

影响两种不同康乃馨原生质体产量和胼胝体诱导的因素的影响
本研究旨在通过考察不同类型的植物组织、酶浓度、培养时间、子叶年龄和培养基成分对原生质体分离、细胞分裂和胼胝体诱导的影响,在两个康乃馨栽培品种 Chabaud 和 Giant Chabaud 中建立一套高效的原生质体分离、细胞分裂和胼胝体诱导方案。我们的研究结果表明,不同植物组织的原生质体产量差异很大,其中真叶的产量和存活率最高,尤其是在使用浓度为 0.1% 的 driselase 和培养时间为 6 小时的情况下。我们观察到,将 driselase 浓度提高到 1.0% 会显著降低所有测试组织的原生质体产量。此外,子叶的年龄对原生质体产量也有明显影响,较年轻的子叶产量更高。事实证明,添加了 1 毫克/升玉米素和 1 毫克/升 2,4-二氯苯氧乙酸(2,4-D)的 Murashige 和 Skoog 培养基对促进子叶和真叶原生质体的细胞分裂和菌落形成最有效。研究还发现,植物生长调节剂(PGRs)对胼胝体增殖有显著影响,不同原生质体来源的影响也不同。
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来源期刊
Plant Biotechnology Reports
Plant Biotechnology Reports 生物-生物工程与应用微生物
CiteScore
4.10
自引率
4.20%
发文量
72
审稿时长
>12 weeks
期刊介绍: Plant Biotechnology Reports publishes original, peer-reviewed articles dealing with all aspects of fundamental and applied research in the field of plant biotechnology, which includes molecular biology, genetics, biochemistry, cell and tissue culture, production of secondary metabolites, metabolic engineering, genomics, proteomics, and metabolomics. Plant Biotechnology Reports emphasizes studies on plants indigenous to the Asia-Pacific region and studies related to commercialization of plant biotechnology. Plant Biotechnology Reports does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.
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