Plant Biotechnology Reports最新文献

Form-associated quality variation and exploratory transcriptome-metabolome characteristics in selected Gastrodia elata tuber samples 天麻块茎样品中形态相关质量变异和探索性转录组-代谢组特征

4区生物学

Plant Biotechnology Reports Pub Date : 2026-07-29 DOI: 10.1007/s11816-026-01075-2

Rui Yang, Fulai Luo, Ping Wen

引用次数: 0

Microstructure analysis contributes to selection of stem cell-rich explants and identification of embryogenic callus in oil palm tissue culture 显微结构分析有助于油棕组织培养中富含干细胞的外植体的选择和胚性愈伤组织的鉴定

4区生物学

Plant Biotechnology Reports Pub Date : 2026-05-13 DOI: 10.1007/s11816-026-01061-8

Jing Yang, Dongxia Li, Peng Shi, Zhiying Li, Dapeng Zhang, Yin Min Htwe, Qun Yu, Xuan He, Yong Wang

引用次数: 0

Precision microneedle-assisted wounding enhances regeneration and enables efficient Agrobacterium-mediated transformation in carnation (Dianthus caryophyllus L.) 精准微针辅助损伤可促进石竹再生，实现农杆菌介导的高效转化。

4区生物学

Plant Biotechnology Reports Pub Date : 2026-02-17 DOI: 10.1007/s11816-026-01049-4

Jun Yang, Chang Kil Kim

{"title":"Precision microneedle-assisted wounding enhances regeneration and enables efficient Agrobacterium-mediated transformation in carnation (Dianthus caryophyllus L.)","authors":"Jun Yang, Chang Kil Kim","doi":"10.1007/s11816-026-01049-4","DOIUrl":"https://doi.org/10.1007/s11816-026-01049-4","url":null,"abstract":"Carnation (Dianthus caryophyllus L.) is an important ornamental crop, but its molecular breeding is limited by low regeneration competence and inefficient Agrobacterium-mediated transformation. This study aimed to establish a reproducible transformation framework for carnation by integrating optimized regeneration conditions with precision microneedle-assisted wounding and infection strategies. Significant genotype-dependent variation in regeneration capacity and auxin responsiveness was observed among six cultivars. Targeted microneedle-assisted wounding at the leaf-base vein junction markedly enhanced direct shoot regeneration, resulting in a 2.27-fold increase in shoot number per explant and earlier regeneration compared with non-wounded controls. Among three infection strategies tested, immersion-wounding with sterile microneedles followed by bacterial immersion produced the highest regeneration frequency (60.0%) and shoot formation. Optimization of non-ionic surfactants revealed that Tween 20 (0.01%) provided a broad and stable operational window, whereas Triton X-100 showed strong concentration-dependent phytotoxicity. Using the optimized protocol, five independent acdS-transgenic carnation lines were successfully recovered, corresponding to a PCR-verified transformation efficiency of 16.67% on an explant basis. PCR and qRT-PCR analyses confirmed stable transgene integration and expression without adverse effects on plant morphology. These results demonstrate that precision microneedle-assisted wounding is a key enabling factor for efficient regeneration and facilitates Agrobacterium-mediated transformation in carnation, providing a practical platform for molecular breeding of regeneration-recalcitrant ornamental crops.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"20 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11816-026-01049-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147381892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive morphological, biochemical, and internal transcribed spacer-based genetic diversity analysis of Daucus spp. genotypes 基于形态学、生物化学和内部转录间隔区基因型的综合遗传多样性分析

4区生物学

Plant Biotechnology Reports Pub Date : 2025-06-27 DOI: 10.1007/s11816-025-00998-6

Sameena Lone, Sumati Narayan, Khalid Z. Masoodi, Muzaffar Malik, Farooq Ahmad Khan, Khairiah Mubarak Alwutayd, Carmen Vlădulescu, Manh Cuong, Khursheed Hussain

引用次数: 0

Overexpression of CRK4, the cysteine-rich receptor-like protein kinase of Arabidopsis, regulates the resistance to abiotic stress and abscisic acid responses 拟南芥富半胱氨酸受体样蛋白激酶 CRK4 的过表达调控对非生物胁迫的抗性和脱落酸反应

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-09-19 DOI: 10.1007/s11816-024-00926-0

Ye-Jin Jang, Sung-Dug Oh, Kangmin Kim, Seong-Kon Lee, Ancheol Chang, Doh-Won Yun, Chul Min Kim, Bumkyu Lee

{"title":"Overexpression of CRK4, the cysteine-rich receptor-like protein kinase of Arabidopsis, regulates the resistance to abiotic stress and abscisic acid responses","authors":"Ye-Jin Jang, Sung-Dug Oh, Kangmin Kim, Seong-Kon Lee, Ancheol Chang, Doh-Won Yun, Chul Min Kim, Bumkyu Lee","doi":"10.1007/s11816-024-00926-0","DOIUrl":"https://doi.org/10.1007/s11816-024-00926-0","url":null,"abstract":"Receptor-like kinases (RLKs) are known to regulate plant growth, cell differentiation, and defense mechanisms. Cysteine-rich receptor-like protein kinases (CRKs) are a subfamily of RLKs with more than 40 members. In this study, we found that overexpressing CRK4 in Arabidopsis enhances abiotic tolerance to salinity and drought stress. We constructed a CRK4-overexpressed pCB302ES plasmid, transformed it into Arabidopsis and evaluated the insertion site of the transgene. The CRK4-overexpressed line was selected for molecular analyses, including western blotting and flanking T-DNA sequencing. In pots treated with either 200 mM NaCl or dryness, the CRK4-overexpressed line showed higher resistance than the wild-type Columbia-0 (Col-0). The expression levels of DREB2B, RAB18, and RD29B genes associated with plant stress, were lower than those of wild-type Col-0. In addition, ABF1 and abscisic-acid-responsive element-binding factor 1 expression levels increased in CRK4-overexpressed transgenic Arabidopsis. Furthermore, in an abscisic acid (ABA)-containing medium, CRK4-overexpressed transgenic Arabidopsis and wild-type Col-0 showed different root development patterns. Our results indicate that CRK4 modulates the ABA-signaling pathway and responds to salt and drought stress in Arabidopsis. This study helps elucidate the correlation between the ABA and CRK4 genes.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"189 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142263230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of a novel Wx-B1 allele in a waxy wheat (Triticum aestivum L.) 蜡质小麦（Triticum aestivum L.）中一种新型 Wx-B1 等位基因的鉴定和特征描述

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-09-11 DOI: 10.1007/s11816-024-00925-1

Yeonjun Sung, Keonghoon Kim, Jinhee Park, Seongwook Kang, Chulsoo Park, Seongwoo Cho, Changsoo Kim

{"title":"Identification and characterization of a novel Wx-B1 allele in a waxy wheat (Triticum aestivum L.)","authors":"Yeonjun Sung, Keonghoon Kim, Jinhee Park, Seongwook Kang, Chulsoo Park, Seongwoo Cho, Changsoo Kim","doi":"10.1007/s11816-024-00925-1","DOIUrl":"https://doi.org/10.1007/s11816-024-00925-1","url":null,"abstract":"The composition of starch, which includes amylose and amylopectin, greatly affects the quality and characteristics of flour. The balance between these components is crucial in determining the properties and structure of starch. The waxy (Wx) gene encodes granule-bound starch synthase I (GBSSI), which is responsible for amylose biosynthesis in the endosperm. Gunji-3 was created through a cross between the waxy-type wheat cultivar Shinmichal 1 and the bread wheat cultivar Keumkang. Upon comparing the Wx-B1 allele encoding GBSSI in Gunji-3 with the Wx-B1 allele in Shinmichal 1 and Wx-B1b, a total of four SNPs and one deletion were identified in Gunji-3. Additionally, when comparing amino acid sequences with Wx-B1 alleles, differences at three positions were found, indicating that the mutant carried a new Wx-B1 allele named Wx-B1o. The physicochemical properties of Gunji-3 starch were characterized by a lower amylose content of 2.30% compared to 7.45% in Shinmichal 1 and 27.67% in Keumkang. Additionally, it exhibited a higher water retention capacity of 84.66% compared to 75.91% in Shinmichal 1 and 66.07% in Keumkang. The newly introduced waxy-type wheat could provide an essential basis for understanding wheat's starch characteristics and various breeding programs.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"41 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142176326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of a sweetpotato stress tolerance-associated GDP-L-galactose phosphorylase gene (IbGGP1) in response to abiotic stress 甘薯抗逆性相关 GDP-L-半乳糖磷酸化酶基因 (IbGGP1) 对非生物胁迫反应的分子特性分析

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-08-20 DOI: 10.1007/s11816-024-00922-4

Yanxin Yang, Sijie Wang, Qirui Cheng, Xuan Zou, Zhe Yang, Peng Li, Yuan Wang, Dongjing Yang, Ho Soo Kim, Xiaoyun Jia, Lingzhi Li, Sang-Soo Kwak, Wenbin Wang

{"title":"Molecular characterization of a sweetpotato stress tolerance-associated GDP-L-galactose phosphorylase gene (IbGGP1) in response to abiotic stress","authors":"Yanxin Yang, Sijie Wang, Qirui Cheng, Xuan Zou, Zhe Yang, Peng Li, Yuan Wang, Dongjing Yang, Ho Soo Kim, Xiaoyun Jia, Lingzhi Li, Sang-Soo Kwak, Wenbin Wang","doi":"10.1007/s11816-024-00922-4","DOIUrl":"https://doi.org/10.1007/s11816-024-00922-4","url":null,"abstract":"Ascorbic acid (AsA) can participate in the enzymatic and nonenzymatic clearance processes of reactive oxygen species (ROS), thereby enhancing stress tolerance in plants. GDP-L-galactose phosphorylase (GGP) is predicted to be a critical enzyme in the L-galactose route of plant AsA biosynthesis. However, information on the catalytic AsA synthesis and stress-resistance effect of the GGP gene in sweetpotato remains scarce. In this study, the IbGGP1 gene from sweetpotato was successfully isolated. The qRT-PCR determination revealed a distinctly higher expression level of IbGGP1 in sweetpotato flowers, and the gene was induced by multiple stresses, especially in drought, salt, and extreme temperatures. The seed germination, root elongation, and fresh weight were promoted in T3 Arabidopsis IbGGP1-overexpressing lines as compared to wild-type plants under mannitol and salt stresses. The heterologous overexpression of IbGGP1 upregulated the mRNA level of the AtGME and AtGPP genes, and elevated the AsA content and AsA/DHA ratio under soil drought and salt stress. This stress-tolerance phenotype was associated with lower hydrogen peroxide and malondialdehyde content and higher antioxidant enzyme activity. These results indicate that the increased expression of IbGGP1 in Arabidopsis improves tolerance to multiple environmental stresses by promoting AsA biosynthesis and ROS-scavenging system. The functional identification of IbGGP1 provides a new approach for improving stress tolerance to drought and salt in sweetpotato and other species.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"12 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142176366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential expression of sweetpotato nodulin 26-like intrinsic protein (NIP) genes in response to infection with the root knot nematode 甘薯结节素 26 样内在蛋白 (NIP) 基因对根结线虫感染的响应差异表达

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-08-13 DOI: 10.1007/s11816-024-00923-3

Jaewook Kim, Jung-Wook Yang, Kang-Lok Lee, Ki Jung Nam, Donghwan Shim, Yun-Hee Kim

{"title":"Differential expression of sweetpotato nodulin 26-like intrinsic protein (NIP) genes in response to infection with the root knot nematode","authors":"Jaewook Kim, Jung-Wook Yang, Kang-Lok Lee, Ki Jung Nam, Donghwan Shim, Yun-Hee Kim","doi":"10.1007/s11816-024-00923-3","DOIUrl":"https://doi.org/10.1007/s11816-024-00923-3","url":null,"abstract":"Sweetpotato (Ipomoea batatas L.) is susceptible to infection by the root knot nematode (RKN) Meloidogyne incognita, which results in significant reductions in the yield of this important tuber crop worldwide. Previously, transcriptome analysis revealed differential gene expression between the roots of RKN-susceptible and -resistant sweetpotato cultivars after RKN infection, raising the possibility of identifying the genes involved in protection against RKN infection. A number of trait-specific and/or response-specific genes were also identified, including RKN infection-responsive aquaporin protein encoded by the nodulin-26-like intrinsic protein (NIP) gene. Therefore, in this study, we investigated the role of NIP genes in the defense response to RKN infection in susceptible and resistant sweetpotato cultivars. A comparison of the nucleotide sequences of sweetpotato with those of its closely related species, morning glory, revealed a group of NIP genes that could be categorized into four different ortholog groups. These four groups contained several unique genes that showed changes in expression in sweetpotato roots after RKN infection. These results indicate that NIP genes could have a potential role in protecting sweetpotato roots from RKN infection.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"62 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142176367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of key genes regulating macronutrient accumulation and final yield in wheat under potassium deficiency 识别调控缺钾条件下小麦主要营养素积累和最终产量的关键基因

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-08-05 DOI: 10.1007/s11816-024-00916-2

Samar G. Thabet, Fatmah Ahmed Safhi, Ahmad M. Alqudah

{"title":"Identification of key genes regulating macronutrient accumulation and final yield in wheat under potassium deficiency","authors":"Samar G. Thabet, Fatmah Ahmed Safhi, Ahmad M. Alqudah","doi":"10.1007/s11816-024-00916-2","DOIUrl":"https://doi.org/10.1007/s11816-024-00916-2","url":null,"abstract":"Potassium deficiency in wheat can significantly influence the accumulation of other macronutrients and affect various yield traits. Understanding the genetic factors controlling wheat macronutrient accumulation and yield attributes is important for improved nutritional wheat quality and human health under potassium deficiency This study investigated a set of 111 wheat accessions to assess their response to potassium deprivation. The accessions were subjected to two different levels of potassium treatment: moderate (K1) and low (K2). The wheat grains were analyzed for four macronutrients, mainly magnesium (Mg), calcium (Ca), potassium (K), and phosphorus (P), as well as yield attributes, under both treatments. A statistically significant decrease was observed for all assessed minerals and yield traits in wheat accessions under low potassium. Genome-wide association study (GWAS) analysis identified 366 SNP markers that were significantly linked with all assessed macronutrients and yield parameters, regardless of the potassium treatments. Remarkably, 14 genomic regions were identified that exhibited highly significant relationships with all evaluated characteristics under both treatments. Interestingly, the TraesCS1B02G359800 gene was located on chromosome 3B and annotated as protein kinases that harbor the variation of NGS, P, Mg_K2, and Mg_K1. Protein kinases can modulate the activity of ion transporters and channels, such as the High-Affinity K+ Transporter (HKT) family, to enhance the uptake and redistribution of potassium and other macronutrient. Therefore, integrating these genetic insights with modern breeding techniques holds the promise of developing superior wheat varieties that can meet the challenges of global food security.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"1 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141942022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of heat stress-tolerant weedy rice and SNP identification of heat-tolerance-related genes 耐热胁迫杂交水稻的筛选及耐热相关基因的 SNP 鉴定

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-07-31 DOI: 10.1007/s11816-024-00920-6

Mainul Hasan Sarker, Md Hamed Hussain, Ting Xiang Neik, Md Zobaer Hasan, Wei Yee Wee, Hock Siew Tan, Swee-suak Ko, Beng-Kah Song

{"title":"Screening of heat stress-tolerant weedy rice and SNP identification of heat-tolerance-related genes","authors":"Mainul Hasan Sarker, Md Hamed Hussain, Ting Xiang Neik, Md Zobaer Hasan, Wei Yee Wee, Hock Siew Tan, Swee-suak Ko, Beng-Kah Song","doi":"10.1007/s11816-024-00920-6","DOIUrl":"https://doi.org/10.1007/s11816-024-00920-6","url":null,"abstract":"Rice, a staple crop that feeds more than one-third of the world’s population, encounters a wide range of biotic and abiotic stresses due to climate change. Rising temperature is one of the significant abiotic stresses affecting rice productivity worldwide. The development of heat-tolerant rice cultivars is critical in this regard. Weedy rice could potentially serve as a natural resource for genes conferring agronomically important traits beneficial to cultivated rice. However, heat tolerance in both cultivated and weedy rice is still understudied. This study screened a set of 180 weedy rice accessions for heat stress tolerance and further characterised them using genome-wide single-nucleotide polymorphisms (SNPs) analysis. Five heat-tolerant (HT) accessions (MU244, MU235, MU249, MU260 and MU237), along with five heat-susceptible (HS) accessions (MU100, MU114, MU264, MU251 and MU005), were subjected to relative electrical conductivity (REC) test and reactive oxidative species assay (ROS). These tests verified that the five HT accessions performed better under heat stress than their HS counterparts. In addition, whole-genome sequences of three HT (MU235, MU237 and MU066) and four HS (MU100, MU114, MU022 and MU005) accessions were selected for the genome-wide SNPs comparison, revealing substantial amino acid variation in the heat-tolerance-related genes between the HT and HS rice groups. The proposed genes and genome-wide SNP markers may help rice breeders better understand how different rice cultivars respond to heat stress.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"212 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141867977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0