Plant Biotechnology Reports最新文献

筛选
英文 中文
Overexpression of CRK4, the cysteine-rich receptor-like protein kinase of Arabidopsis, regulates the resistance to abiotic stress and abscisic acid responses 拟南芥富半胱氨酸受体样蛋白激酶 CRK4 的过表达调控对非生物胁迫的抗性和脱落酸反应
IF 2.4 4区 生物学
Plant Biotechnology Reports Pub Date : 2024-09-19 DOI: 10.1007/s11816-024-00926-0
Ye-Jin Jang, Sung-Dug Oh, Kangmin Kim, Seong-Kon Lee, Ancheol Chang, Doh-Won Yun, Chul Min Kim, Bumkyu Lee
{"title":"Overexpression of CRK4, the cysteine-rich receptor-like protein kinase of Arabidopsis, regulates the resistance to abiotic stress and abscisic acid responses","authors":"Ye-Jin Jang, Sung-Dug Oh, Kangmin Kim, Seong-Kon Lee, Ancheol Chang, Doh-Won Yun, Chul Min Kim, Bumkyu Lee","doi":"10.1007/s11816-024-00926-0","DOIUrl":"https://doi.org/10.1007/s11816-024-00926-0","url":null,"abstract":"<p>Receptor-like kinases (RLKs) are known to regulate plant growth, cell differentiation, and defense mechanisms. Cysteine-rich receptor-like protein kinases (CRKs) are a subfamily of RLKs with more than 40 members. In this study, we found that overexpressing <i>CRK4</i> in <i>Arabidopsis</i> enhances abiotic tolerance to salinity and drought stress. We constructed a <i>CRK4</i>-overexpressed pCB302ES plasmid, transformed it into <i>Arabidopsis</i> and evaluated the insertion site of the transgene. The <i>CRK4</i>-overexpressed line was selected for molecular analyses, including western blotting and flanking T-DNA sequencing. In pots treated with either 200 mM NaCl or dryness, the <i>CRK4</i>-overexpressed line showed higher resistance than the wild-type Columbia-0 (Col-0). The expression levels of <i>DREB2B</i>, <i>RAB18,</i> and <i>RD29B</i> genes associated with plant stress, were lower than those of wild-type Col-0. In addition, <i>ABF1</i> and abscisic-acid-responsive element-binding factor 1 expression levels increased in <i>CRK4</i>-overexpressed transgenic <i>Arabidopsis</i>. Furthermore, in an abscisic acid (ABA)-containing medium, <i>CRK4</i>-overexpressed transgenic <i>Arabidopsis</i> and wild-type Col-0 showed different root development patterns. Our results indicate that CRK4 modulates the ABA-signaling pathway and responds to salt and drought stress in <i>Arabidopsis</i>. This study helps elucidate the correlation between the ABA and CRK4 genes.</p>","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"189 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142263230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification and characterization of a novel Wx-B1 allele in a waxy wheat (Triticum aestivum L.) 蜡质小麦(Triticum aestivum L.)中一种新型 Wx-B1 等位基因的鉴定和特征描述
IF 2.4 4区 生物学
Plant Biotechnology Reports Pub Date : 2024-09-11 DOI: 10.1007/s11816-024-00925-1
Yeonjun Sung, Keonghoon Kim, Jinhee Park, Seongwook Kang, Chulsoo Park, Seongwoo Cho, Changsoo Kim
{"title":"Identification and characterization of a novel Wx-B1 allele in a waxy wheat (Triticum aestivum L.)","authors":"Yeonjun Sung, Keonghoon Kim, Jinhee Park, Seongwook Kang, Chulsoo Park, Seongwoo Cho, Changsoo Kim","doi":"10.1007/s11816-024-00925-1","DOIUrl":"https://doi.org/10.1007/s11816-024-00925-1","url":null,"abstract":"<p>The composition of starch, which includes amylose and amylopectin, greatly affects the quality and characteristics of flour. The balance between these components is crucial in determining the properties and structure of starch. The waxy (<i>Wx</i>) gene encodes granule-bound starch synthase I (GBSSI), which is responsible for amylose biosynthesis in the endosperm. Gunji-3 was created through a cross between the waxy-type wheat cultivar Shinmichal 1 and the bread wheat cultivar Keumkang. Upon comparing the <i>Wx-B1</i> allele encoding GBSSI in Gunji-3 with the <i>Wx-B1</i> allele in Shinmichal 1 and <i>Wx-B1b</i>, a total of four SNPs and one deletion were identified in Gunji-3. Additionally, when comparing amino acid sequences with <i>Wx-B1</i> alleles, differences at three positions were found, indicating that the mutant carried a new <i>Wx-B1</i> allele named <i>Wx-B1o</i>. The physicochemical properties of Gunji-3 starch were characterized by a lower amylose content of 2.30% compared to 7.45% in Shinmichal 1 and 27.67% in Keumkang. Additionally, it exhibited a higher water retention capacity of 84.66% compared to 75.91% in Shinmichal 1 and 66.07% in Keumkang. The newly introduced waxy-type wheat could provide an essential basis for understanding wheat's starch characteristics and various breeding programs.</p>","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"41 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142176326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular characterization of a sweetpotato stress tolerance-associated GDP-L-galactose phosphorylase gene (IbGGP1) in response to abiotic stress 甘薯抗逆性相关 GDP-L-半乳糖磷酸化酶基因 (IbGGP1) 对非生物胁迫反应的分子特性分析
IF 2.4 4区 生物学
Plant Biotechnology Reports Pub Date : 2024-08-20 DOI: 10.1007/s11816-024-00922-4
Yanxin Yang, Sijie Wang, Qirui Cheng, Xuan Zou, Zhe Yang, Peng Li, Yuan Wang, Dongjing Yang, Ho Soo Kim, Xiaoyun Jia, Lingzhi Li, Sang-Soo Kwak, Wenbin Wang
{"title":"Molecular characterization of a sweetpotato stress tolerance-associated GDP-L-galactose phosphorylase gene (IbGGP1) in response to abiotic stress","authors":"Yanxin Yang, Sijie Wang, Qirui Cheng, Xuan Zou, Zhe Yang, Peng Li, Yuan Wang, Dongjing Yang, Ho Soo Kim, Xiaoyun Jia, Lingzhi Li, Sang-Soo Kwak, Wenbin Wang","doi":"10.1007/s11816-024-00922-4","DOIUrl":"https://doi.org/10.1007/s11816-024-00922-4","url":null,"abstract":"<p>Ascorbic acid (AsA) can participate in the enzymatic and nonenzymatic clearance processes of reactive oxygen species (ROS), thereby enhancing stress tolerance in plants. GDP-L-galactose phosphorylase (GGP) is predicted to be a critical enzyme in the L-galactose route of plant AsA biosynthesis. However, information on the catalytic AsA synthesis and stress-resistance effect of the <i>GGP</i> gene in sweetpotato remains scarce. In this study, the <i>IbGGP1</i> gene from sweetpotato was successfully isolated. The qRT-PCR determination revealed a distinctly higher expression level of <i>IbGGP1</i> in sweetpotato flowers, and the gene was induced by multiple stresses, especially in drought, salt, and extreme temperatures. The seed germination, root elongation, and fresh weight were promoted in T<sub>3</sub> <i>Arabidopsis IbGGP1</i>-overexpressing lines as compared to wild-type plants under mannitol and salt stresses. The heterologous overexpression of <i>IbGGP1</i> upregulated the mRNA level of the <i>AtGME</i> and <i>AtGPP</i> genes, and elevated the AsA content and AsA/DHA ratio under soil drought and salt stress. This stress-tolerance phenotype was associated with lower hydrogen peroxide and malondialdehyde content and higher antioxidant enzyme activity. These results indicate that the increased expression of <i>IbGGP1</i> in <i>Arabidopsis</i> improves tolerance to multiple environmental stresses by promoting AsA biosynthesis and ROS-scavenging system. The functional identification of <i>IbGGP1</i> provides a new approach for improving stress tolerance to drought and salt in sweetpotato and other species.</p>","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"12 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142176366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential expression of sweetpotato nodulin 26-like intrinsic protein (NIP) genes in response to infection with the root knot nematode 甘薯结节素 26 样内在蛋白 (NIP) 基因对根结线虫感染的响应差异表达
IF 2.4 4区 生物学
Plant Biotechnology Reports Pub Date : 2024-08-13 DOI: 10.1007/s11816-024-00923-3
Jaewook Kim, Jung-Wook Yang, Kang-Lok Lee, Ki Jung Nam, Donghwan Shim, Yun-Hee Kim
{"title":"Differential expression of sweetpotato nodulin 26-like intrinsic protein (NIP) genes in response to infection with the root knot nematode","authors":"Jaewook Kim, Jung-Wook Yang, Kang-Lok Lee, Ki Jung Nam, Donghwan Shim, Yun-Hee Kim","doi":"10.1007/s11816-024-00923-3","DOIUrl":"https://doi.org/10.1007/s11816-024-00923-3","url":null,"abstract":"<p>Sweetpotato (<i>Ipomoea batatas L.</i>) is susceptible to infection by the root knot nematode (RKN) <i>Meloidogyne incognita</i>, which results in significant reductions in the yield of this important tuber crop worldwide. Previously, transcriptome analysis revealed differential gene expression between the roots of RKN-susceptible and -resistant sweetpotato cultivars after RKN infection, raising the possibility of identifying the genes involved in protection against RKN infection. A number of trait-specific and/or response-specific genes were also identified, including RKN infection-responsive aquaporin protein encoded by the nodulin-26-like intrinsic protein (NIP) gene. Therefore, in this study, we investigated the role of <i>NIP</i> genes in the defense response to RKN infection in susceptible and resistant sweetpotato cultivars. A comparison of the nucleotide sequences of sweetpotato with those of its closely related species, morning glory, revealed a group of <i>NIP</i> genes that could be categorized into four different ortholog groups. These four groups contained several unique genes that showed changes in expression in sweetpotato roots after RKN infection. These results indicate that <i>NIP</i> genes could have a potential role in protecting sweetpotato roots from RKN infection.</p>","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"62 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142176367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of key genes regulating macronutrient accumulation and final yield in wheat under potassium deficiency 识别调控缺钾条件下小麦主要营养素积累和最终产量的关键基因
IF 2.4 4区 生物学
Plant Biotechnology Reports Pub Date : 2024-08-05 DOI: 10.1007/s11816-024-00916-2
Samar G. Thabet, Fatmah Ahmed Safhi, Ahmad M. Alqudah
{"title":"Identification of key genes regulating macronutrient accumulation and final yield in wheat under potassium deficiency","authors":"Samar G. Thabet, Fatmah Ahmed Safhi, Ahmad M. Alqudah","doi":"10.1007/s11816-024-00916-2","DOIUrl":"https://doi.org/10.1007/s11816-024-00916-2","url":null,"abstract":"<p>Potassium deficiency in wheat can significantly influence the accumulation of other macronutrients and affect various yield traits. Understanding the genetic factors controlling wheat macronutrient accumulation and yield attributes is important for improved nutritional wheat quality and human health under potassium deficiency This study investigated a set of 111 wheat accessions to assess their response to potassium deprivation. The accessions were subjected to two different levels of potassium treatment: moderate (K1) and low (K2). The wheat grains were analyzed for four macronutrients, mainly magnesium (Mg), calcium (Ca), potassium (K), and phosphorus (P), as well as yield attributes, under both treatments. A statistically significant decrease was observed for all assessed minerals and yield traits in wheat accessions under low potassium. Genome-wide association study (GWAS) analysis identified 366 SNP markers that were significantly linked with all assessed macronutrients and yield parameters, regardless of the potassium treatments. Remarkably, 14 genomic regions were identified that exhibited highly significant relationships with all evaluated characteristics under both treatments. Interestingly, the <i>TraesCS1B02G359800</i> gene was located on chromosome 3B and annotated as protein kinases that harbor the variation of NGS, P, Mg_K2, and Mg_K1. Protein kinases can modulate the activity of ion transporters and channels, such as the High-Affinity K<sup>+</sup> Transporter (HKT) family, to enhance the uptake and redistribution of potassium and other macronutrient. Therefore, integrating these genetic insights with modern breeding techniques holds the promise of developing superior wheat varieties that can meet the challenges of global food security.</p>","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"1 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141942022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Screening of heat stress-tolerant weedy rice and SNP identification of heat-tolerance-related genes 耐热胁迫杂交水稻的筛选及耐热相关基因的 SNP 鉴定
IF 2.4 4区 生物学
Plant Biotechnology Reports Pub Date : 2024-07-31 DOI: 10.1007/s11816-024-00920-6
Mainul Hasan Sarker, Md Hamed Hussain, Ting Xiang Neik, Md Zobaer Hasan, Wei Yee Wee, Hock Siew Tan, Swee-suak Ko, Beng-Kah Song
{"title":"Screening of heat stress-tolerant weedy rice and SNP identification of heat-tolerance-related genes","authors":"Mainul Hasan Sarker, Md Hamed Hussain, Ting Xiang Neik, Md Zobaer Hasan, Wei Yee Wee, Hock Siew Tan, Swee-suak Ko, Beng-Kah Song","doi":"10.1007/s11816-024-00920-6","DOIUrl":"https://doi.org/10.1007/s11816-024-00920-6","url":null,"abstract":"<p>Rice, a staple crop that feeds more than one-third of the world’s population, encounters a wide range of biotic and abiotic stresses due to climate change. Rising temperature is one of the significant abiotic stresses affecting rice productivity worldwide. The development of heat-tolerant rice cultivars is critical in this regard. Weedy rice could potentially serve as a natural resource for genes conferring agronomically important traits beneficial to cultivated rice. However, heat tolerance in both cultivated and weedy rice is still understudied. This study screened a set of 180 weedy rice accessions for heat stress tolerance and further characterised them using genome-wide single-nucleotide polymorphisms (SNPs) analysis. Five heat-tolerant (HT) accessions (MU244, MU235, MU249, MU260 and MU237), along with five heat-susceptible (HS) accessions (MU100, MU114, MU264, MU251 and MU005), were subjected to relative electrical conductivity (REC) test and reactive oxidative species assay (ROS). These tests verified that the five HT accessions performed better under heat stress than their HS counterparts. In addition, whole-genome sequences of three HT (MU235, MU237 and MU066) and four HS (MU100, MU114, MU022 and MU005) accessions were selected for the genome-wide SNPs comparison, revealing substantial amino acid variation in the heat-tolerance-related genes between the HT and HS rice groups. The proposed genes and genome-wide SNP markers may help rice breeders better understand how different rice cultivars respond to heat stress.</p>","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"212 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141867977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular characteristics and phylogenetic definition on the complete chloroplast genome of Petrocodon longitubus 长舌石龙子完整叶绿体基因组的分子特征和系统发育定义
IF 2.4 4区 生物学
Plant Biotechnology Reports Pub Date : 2024-07-31 DOI: 10.1007/s11816-024-00919-z
Zaiqi Luo, FengXia Yan, Ronghui Jiang, Yanjun Chen, Changsha Luo, CongRui Li
{"title":"Molecular characteristics and phylogenetic definition on the complete chloroplast genome of Petrocodon longitubus","authors":"Zaiqi Luo, FengXia Yan, Ronghui Jiang, Yanjun Chen, Changsha Luo, CongRui Li","doi":"10.1007/s11816-024-00919-z","DOIUrl":"https://doi.org/10.1007/s11816-024-00919-z","url":null,"abstract":"<p><i>Petrocodon</i> is a small genus in the family Gesneriaceae, which is special for its remarkable floral diversity, and has high ornamental value. In this study, the complete chloroplast genome sequence and genome characteristics of <i>Petrocodon longitubus</i> are first reported. The genome size is 152,958 bp, including a large single-copy region (LSC, 83,901 bp), a small single-copy region (SSC, 18,255 bp), and two inverted repeat sequences (IRs, 25,401 bp, each). The chloroplast genome of P. longitubus was analyzed, revealing a total GC content of 37.47%. A total of 131 genes were de novo assembled, consisting of 87 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. A comparative analysis was conducted between the chloroplast genome of <i>P. longitubus</i> and three other published species of <i>Petrocodon</i>. The chloroplast genome of four <i>Petrocodon</i> species was found to have a double-chain ring structure, with a size ranging from 152,958 to 153,292 bp. Chloroplast genome size had indistinguishable. Four <i>Petrocodon</i> species was ra elatively conserved sequence, with 87 or 88 protein-coding genes, and 8 rRNA were the most conserved, which contains 42 ~ 50 SSR sites, which are mainly mononucleotides and dinucleotides, 4 boundary transition regions, then trinucleotides, pentanucleotides and hexanucleotides have been not detected. The non-preferred codons of the chloroplast genome in the four Petrocodo<i>n</i> species are those ending in A, C, G, or T. The chloroplast genomes of these four Petrocodon species are highly similar to each other and to several <i>Primulina</i> species. Phylogenetic trees indicate that <i>P. longitubus</i> and other <i>Petrocodon</i> species were grouped together in a clade, with <i>P. longitubus</i> form a single clade. The results support the scientific naming of <i>P. Longitubusr</i> based on horticultural traits and further clarify the systematic status using molecular information.</p>","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"35 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141867976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enlightening the biotechnological approaches of legumes: present and past scenario to mitigate the biotic and abiotic stress 揭示豆科植物的生物技术方法:缓解生物和非生物胁迫的现状和前景
IF 2.4 4区 生物学
Plant Biotechnology Reports Pub Date : 2024-07-24 DOI: 10.1007/s11816-024-00921-5
Janani Sree Sivakumar, Dhandapani Gurusamy, Selvakumar Subramaniam, Vasanth Krishnan
{"title":"Enlightening the biotechnological approaches of legumes: present and past scenario to mitigate the biotic and abiotic stress","authors":"Janani Sree Sivakumar, Dhandapani Gurusamy, Selvakumar Subramaniam, Vasanth Krishnan","doi":"10.1007/s11816-024-00921-5","DOIUrl":"https://doi.org/10.1007/s11816-024-00921-5","url":null,"abstract":"<p>Legumes have played an essential part as food and feed, but their productivity is inadequate to deliver the protein needs of a rapidly growing human population and livestock sectors. In numerous places around the world, legumes are the peculiar source of protein which essential for the human consumption. They are typically used as a supplement to other dietary proteins. As legumes have the richest protein source, the health experts and consumers demand increased rapidly. Simultaneously, the inhabitants of humans in the universe increased promptly. Meanwhile, fluctuation in biotic and also abiotic components is an extensive constraint for crop growth. It may provoke stress which results in the reduction of yield. However, the legume shows recalcitrant nature, these are the major root cause of the shortage of food. The biotechnology provides a solution to rectify all those issues and genetic transformation plays a consequential impact on enhancement of the legume productivity. This review clearly emphasizes the constraints of legume productivity, the strategy used, skill requirement and current advances in biotechnology for legume improvement.</p>","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"7 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141778089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring secondary structures within the nuclear ribosomal DNA internal transcribed spacers (nr DNA ITS) for enhanced phylogenetic insights in Medicago sativa (alfalfa) 探索核糖体 DNA 内部转录间隔(nr DNA ITS)中的二级结构,提高对紫花苜蓿(Medicago sativa)系统发育的认识
IF 2.4 4区 生物学
Plant Biotechnology Reports Pub Date : 2024-07-13 DOI: 10.1007/s11816-024-00915-3
M. Alp Furan, Gulistan Genli, Mehtap Yıldız, Hyeon-Jin Sun
{"title":"Exploring secondary structures within the nuclear ribosomal DNA internal transcribed spacers (nr DNA ITS) for enhanced phylogenetic insights in Medicago sativa (alfalfa)","authors":"M. Alp Furan, Gulistan Genli, Mehtap Yıldız, Hyeon-Jin Sun","doi":"10.1007/s11816-024-00915-3","DOIUrl":"https://doi.org/10.1007/s11816-024-00915-3","url":null,"abstract":"<p>ITS sections, which are situated inside the ribosomal DNA operon, are recognized for their high levels of diversity and slow rate of evolution, which makes them important for plant phylogenetic studies. The objective of this work is to use the internal transcribed spacer (ITS) regions of 18 alfalfa (<i>Medicago sativa</i>) genotypes/varieties to determine their genetic relationships and to verify possible genetic markers for variety identification and classification. This has been done by validating the secondary structures of the ITS regions and observing the effects of the relationships among the lowest energy state, the total number of possible secondary structure hairpins, and (GC) content. The results indicated that the length of the ITS sequences varied between 426 and 629 nucleotides and that the G + C content of the ITS region varied between 46.6 and 50%. In this study, the analysis of molecular variance (AMOVA) was used to validate the effectiveness of configuration analysis and revealed that fluctuations in the data accounted for a substantial amount of the observed total variance. Moreover, 94 percent of the genotypes analyzed exhibited a significant degree of variety. This shows that a large fraction of the observed variation is likely attributable to genetic variables. Population four, comprising Bilensoy80, Emily, Quin, Vendor, and Felicia, had the highest degree of heterozygosity at 68.8%, whereas population two, including Gozlu1, Prosementi, Nimet and Local (Van), had the lowest level at 37.5%. The original Shannon’s approach as an unbiased estimator employed in population genetics research validated the differences among alfalfa genotypes with the AMOVA analysis results of this study. Individual differences were found to be 59%, whereas population differences were found to be 41%. There have been a few ITS studies on <i>Medicago sativa</i> that have utilized ITS as a phylogenetic marker to estimate connections and define new taxonomic categories (e.g., tribes). However, our research also includes an analysis of the secondary structure of these sequences and the results of this study imply that ITS sequence and secondary structure data can be utilized to understand the intraspecific genetic makeup of different alfalfa varieties.</p>","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"46 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141614676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sorghum-encoded microRNAs: current status and future prospects 高粱编码的 microRNA:现状与前景
IF 2.4 4区 生物学
Plant Biotechnology Reports Pub Date : 2024-07-11 DOI: 10.1007/s11816-024-00914-4
Rekha Yadav, Chandra Pal Singh
{"title":"Sorghum-encoded microRNAs: current status and future prospects","authors":"Rekha Yadav, Chandra Pal Singh","doi":"10.1007/s11816-024-00914-4","DOIUrl":"https://doi.org/10.1007/s11816-024-00914-4","url":null,"abstract":"<p>Sorghum (<i>Sorghum bicolor</i> (L.) Moench) is an annual or short-term perennial plant belonging to the economically important family Poaceae. Sorghum, a C4 crop, has multiple uses like food, fodder, forage, and also as a biofuel feedstock. With an ability to thrive under harsh environmental conditions and adaptability to diverse climates and soils, sorghum has a long history of cultivation in the semi-arid tropics of Africa, Asia, and Latin America. Gene regulation plays an important role in adaptability to adverse environmental conditions. MicroRNAs (miRNAs) are one of the classes of small non-coding RNAs that have emerged as a key regulators of gene expression. These small RNAs are profoundly present in all higher eukaryotes including plants. These are involved in regulating the intrinsic normal growth of cells and the development of organisms as well as in maintaining the integrity of genomes. In plants, miRNAs have been functionally implicated in abiotic stress tolerance, flower development, root development, grain size determination, yield, and immune responses. Several miRNAs have been reported in sorghum, and the potential functions of some miRNAs have been characterized. Here, in this review, an overview of sorghum-encoded miRNAs is provided. The potential known and putative functions of these miRNAs are critically discussed. In addition, the possible methods for employing miRNAs as a tool for sorghum improvement are also suggested. The present review will help us to understand the miRNA functions in sorghum and underlying regulatory gene networks which have applications to design effective methods to achieve desired traits.</p>","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"41 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141610921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信