Plant Biotechnology Reports最新文献

Overexpression of CRK4, the cysteine-rich receptor-like protein kinase of Arabidopsis, regulates the resistance to abiotic stress and abscisic acid responses 拟南芥富半胱氨酸受体样蛋白激酶 CRK4 的过表达调控对非生物胁迫的抗性和脱落酸反应

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-09-19 DOI: 10.1007/s11816-024-00926-0

Ye-Jin Jang, Sung-Dug Oh, Kangmin Kim, Seong-Kon Lee, Ancheol Chang, Doh-Won Yun, Chul Min Kim, Bumkyu Lee

{"title":"Overexpression of CRK4, the cysteine-rich receptor-like protein kinase of Arabidopsis, regulates the resistance to abiotic stress and abscisic acid responses","authors":"Ye-Jin Jang, Sung-Dug Oh, Kangmin Kim, Seong-Kon Lee, Ancheol Chang, Doh-Won Yun, Chul Min Kim, Bumkyu Lee","doi":"10.1007/s11816-024-00926-0","DOIUrl":"https://doi.org/10.1007/s11816-024-00926-0","url":null,"abstract":"Receptor-like kinases (RLKs) are known to regulate plant growth, cell differentiation, and defense mechanisms. Cysteine-rich receptor-like protein kinases (CRKs) are a subfamily of RLKs with more than 40 members. In this study, we found that overexpressing CRK4 in Arabidopsis enhances abiotic tolerance to salinity and drought stress. We constructed a CRK4-overexpressed pCB302ES plasmid, transformed it into Arabidopsis and evaluated the insertion site of the transgene. The CRK4-overexpressed line was selected for molecular analyses, including western blotting and flanking T-DNA sequencing. In pots treated with either 200 mM NaCl or dryness, the CRK4-overexpressed line showed higher resistance than the wild-type Columbia-0 (Col-0). The expression levels of DREB2B, RAB18, and RD29B genes associated with plant stress, were lower than those of wild-type Col-0. In addition, ABF1 and abscisic-acid-responsive element-binding factor 1 expression levels increased in CRK4-overexpressed transgenic Arabidopsis. Furthermore, in an abscisic acid (ABA)-containing medium, CRK4-overexpressed transgenic Arabidopsis and wild-type Col-0 showed different root development patterns. Our results indicate that CRK4 modulates the ABA-signaling pathway and responds to salt and drought stress in Arabidopsis. This study helps elucidate the correlation between the ABA and CRK4 genes.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142263230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of a novel Wx-B1 allele in a waxy wheat (Triticum aestivum L.) 蜡质小麦（Triticum aestivum L.）中一种新型 Wx-B1 等位基因的鉴定和特征描述

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-09-11 DOI: 10.1007/s11816-024-00925-1

Yeonjun Sung, Keonghoon Kim, Jinhee Park, Seongwook Kang, Chulsoo Park, Seongwoo Cho, Changsoo Kim

{"title":"Identification and characterization of a novel Wx-B1 allele in a waxy wheat (Triticum aestivum L.)","authors":"Yeonjun Sung, Keonghoon Kim, Jinhee Park, Seongwook Kang, Chulsoo Park, Seongwoo Cho, Changsoo Kim","doi":"10.1007/s11816-024-00925-1","DOIUrl":"https://doi.org/10.1007/s11816-024-00925-1","url":null,"abstract":"The composition of starch, which includes amylose and amylopectin, greatly affects the quality and characteristics of flour. The balance between these components is crucial in determining the properties and structure of starch. The waxy (Wx) gene encodes granule-bound starch synthase I (GBSSI), which is responsible for amylose biosynthesis in the endosperm. Gunji-3 was created through a cross between the waxy-type wheat cultivar Shinmichal 1 and the bread wheat cultivar Keumkang. Upon comparing the Wx-B1 allele encoding GBSSI in Gunji-3 with the Wx-B1 allele in Shinmichal 1 and Wx-B1b, a total of four SNPs and one deletion were identified in Gunji-3. Additionally, when comparing amino acid sequences with Wx-B1 alleles, differences at three positions were found, indicating that the mutant carried a new Wx-B1 allele named Wx-B1o. The physicochemical properties of Gunji-3 starch were characterized by a lower amylose content of 2.30% compared to 7.45% in Shinmichal 1 and 27.67% in Keumkang. Additionally, it exhibited a higher water retention capacity of 84.66% compared to 75.91% in Shinmichal 1 and 66.07% in Keumkang. The newly introduced waxy-type wheat could provide an essential basis for understanding wheat's starch characteristics and various breeding programs.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142176326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of a sweetpotato stress tolerance-associated GDP-L-galactose phosphorylase gene (IbGGP1) in response to abiotic stress 甘薯抗逆性相关 GDP-L-半乳糖磷酸化酶基因 (IbGGP1) 对非生物胁迫反应的分子特性分析

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-08-20 DOI: 10.1007/s11816-024-00922-4

Yanxin Yang, Sijie Wang, Qirui Cheng, Xuan Zou, Zhe Yang, Peng Li, Yuan Wang, Dongjing Yang, Ho Soo Kim, Xiaoyun Jia, Lingzhi Li, Sang-Soo Kwak, Wenbin Wang

{"title":"Molecular characterization of a sweetpotato stress tolerance-associated GDP-L-galactose phosphorylase gene (IbGGP1) in response to abiotic stress","authors":"Yanxin Yang, Sijie Wang, Qirui Cheng, Xuan Zou, Zhe Yang, Peng Li, Yuan Wang, Dongjing Yang, Ho Soo Kim, Xiaoyun Jia, Lingzhi Li, Sang-Soo Kwak, Wenbin Wang","doi":"10.1007/s11816-024-00922-4","DOIUrl":"https://doi.org/10.1007/s11816-024-00922-4","url":null,"abstract":"Ascorbic acid (AsA) can participate in the enzymatic and nonenzymatic clearance processes of reactive oxygen species (ROS), thereby enhancing stress tolerance in plants. GDP-L-galactose phosphorylase (GGP) is predicted to be a critical enzyme in the L-galactose route of plant AsA biosynthesis. However, information on the catalytic AsA synthesis and stress-resistance effect of the GGP gene in sweetpotato remains scarce. In this study, the IbGGP1 gene from sweetpotato was successfully isolated. The qRT-PCR determination revealed a distinctly higher expression level of IbGGP1 in sweetpotato flowers, and the gene was induced by multiple stresses, especially in drought, salt, and extreme temperatures. The seed germination, root elongation, and fresh weight were promoted in T3 Arabidopsis IbGGP1-overexpressing lines as compared to wild-type plants under mannitol and salt stresses. The heterologous overexpression of IbGGP1 upregulated the mRNA level of the AtGME and AtGPP genes, and elevated the AsA content and AsA/DHA ratio under soil drought and salt stress. This stress-tolerance phenotype was associated with lower hydrogen peroxide and malondialdehyde content and higher antioxidant enzyme activity. These results indicate that the increased expression of IbGGP1 in Arabidopsis improves tolerance to multiple environmental stresses by promoting AsA biosynthesis and ROS-scavenging system. The functional identification of IbGGP1 provides a new approach for improving stress tolerance to drought and salt in sweetpotato and other species.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142176366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential expression of sweetpotato nodulin 26-like intrinsic protein (NIP) genes in response to infection with the root knot nematode 甘薯结节素 26 样内在蛋白 (NIP) 基因对根结线虫感染的响应差异表达

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-08-13 DOI: 10.1007/s11816-024-00923-3

Jaewook Kim, Jung-Wook Yang, Kang-Lok Lee, Ki Jung Nam, Donghwan Shim, Yun-Hee Kim

{"title":"Differential expression of sweetpotato nodulin 26-like intrinsic protein (NIP) genes in response to infection with the root knot nematode","authors":"Jaewook Kim, Jung-Wook Yang, Kang-Lok Lee, Ki Jung Nam, Donghwan Shim, Yun-Hee Kim","doi":"10.1007/s11816-024-00923-3","DOIUrl":"https://doi.org/10.1007/s11816-024-00923-3","url":null,"abstract":"Sweetpotato (Ipomoea batatas L.) is susceptible to infection by the root knot nematode (RKN) Meloidogyne incognita, which results in significant reductions in the yield of this important tuber crop worldwide. Previously, transcriptome analysis revealed differential gene expression between the roots of RKN-susceptible and -resistant sweetpotato cultivars after RKN infection, raising the possibility of identifying the genes involved in protection against RKN infection. A number of trait-specific and/or response-specific genes were also identified, including RKN infection-responsive aquaporin protein encoded by the nodulin-26-like intrinsic protein (NIP) gene. Therefore, in this study, we investigated the role of NIP genes in the defense response to RKN infection in susceptible and resistant sweetpotato cultivars. A comparison of the nucleotide sequences of sweetpotato with those of its closely related species, morning glory, revealed a group of NIP genes that could be categorized into four different ortholog groups. These four groups contained several unique genes that showed changes in expression in sweetpotato roots after RKN infection. These results indicate that NIP genes could have a potential role in protecting sweetpotato roots from RKN infection.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142176367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of key genes regulating macronutrient accumulation and final yield in wheat under potassium deficiency 识别调控缺钾条件下小麦主要营养素积累和最终产量的关键基因

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-08-05 DOI: 10.1007/s11816-024-00916-2

Samar G. Thabet, Fatmah Ahmed Safhi, Ahmad M. Alqudah

{"title":"Identification of key genes regulating macronutrient accumulation and final yield in wheat under potassium deficiency","authors":"Samar G. Thabet, Fatmah Ahmed Safhi, Ahmad M. Alqudah","doi":"10.1007/s11816-024-00916-2","DOIUrl":"https://doi.org/10.1007/s11816-024-00916-2","url":null,"abstract":"Potassium deficiency in wheat can significantly influence the accumulation of other macronutrients and affect various yield traits. Understanding the genetic factors controlling wheat macronutrient accumulation and yield attributes is important for improved nutritional wheat quality and human health under potassium deficiency This study investigated a set of 111 wheat accessions to assess their response to potassium deprivation. The accessions were subjected to two different levels of potassium treatment: moderate (K1) and low (K2). The wheat grains were analyzed for four macronutrients, mainly magnesium (Mg), calcium (Ca), potassium (K), and phosphorus (P), as well as yield attributes, under both treatments. A statistically significant decrease was observed for all assessed minerals and yield traits in wheat accessions under low potassium. Genome-wide association study (GWAS) analysis identified 366 SNP markers that were significantly linked with all assessed macronutrients and yield parameters, regardless of the potassium treatments. Remarkably, 14 genomic regions were identified that exhibited highly significant relationships with all evaluated characteristics under both treatments. Interestingly, the TraesCS1B02G359800 gene was located on chromosome 3B and annotated as protein kinases that harbor the variation of NGS, P, Mg_K2, and Mg_K1. Protein kinases can modulate the activity of ion transporters and channels, such as the High-Affinity K+ Transporter (HKT) family, to enhance the uptake and redistribution of potassium and other macronutrient. Therefore, integrating these genetic insights with modern breeding techniques holds the promise of developing superior wheat varieties that can meet the challenges of global food security.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141942022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of heat stress-tolerant weedy rice and SNP identification of heat-tolerance-related genes 耐热胁迫杂交水稻的筛选及耐热相关基因的 SNP 鉴定

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-07-31 DOI: 10.1007/s11816-024-00920-6

Mainul Hasan Sarker, Md Hamed Hussain, Ting Xiang Neik, Md Zobaer Hasan, Wei Yee Wee, Hock Siew Tan, Swee-suak Ko, Beng-Kah Song

{"title":"Screening of heat stress-tolerant weedy rice and SNP identification of heat-tolerance-related genes","authors":"Mainul Hasan Sarker, Md Hamed Hussain, Ting Xiang Neik, Md Zobaer Hasan, Wei Yee Wee, Hock Siew Tan, Swee-suak Ko, Beng-Kah Song","doi":"10.1007/s11816-024-00920-6","DOIUrl":"https://doi.org/10.1007/s11816-024-00920-6","url":null,"abstract":"Rice, a staple crop that feeds more than one-third of the world’s population, encounters a wide range of biotic and abiotic stresses due to climate change. Rising temperature is one of the significant abiotic stresses affecting rice productivity worldwide. The development of heat-tolerant rice cultivars is critical in this regard. Weedy rice could potentially serve as a natural resource for genes conferring agronomically important traits beneficial to cultivated rice. However, heat tolerance in both cultivated and weedy rice is still understudied. This study screened a set of 180 weedy rice accessions for heat stress tolerance and further characterised them using genome-wide single-nucleotide polymorphisms (SNPs) analysis. Five heat-tolerant (HT) accessions (MU244, MU235, MU249, MU260 and MU237), along with five heat-susceptible (HS) accessions (MU100, MU114, MU264, MU251 and MU005), were subjected to relative electrical conductivity (REC) test and reactive oxidative species assay (ROS). These tests verified that the five HT accessions performed better under heat stress than their HS counterparts. In addition, whole-genome sequences of three HT (MU235, MU237 and MU066) and four HS (MU100, MU114, MU022 and MU005) accessions were selected for the genome-wide SNPs comparison, revealing substantial amino acid variation in the heat-tolerance-related genes between the HT and HS rice groups. The proposed genes and genome-wide SNP markers may help rice breeders better understand how different rice cultivars respond to heat stress.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141867977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characteristics and phylogenetic definition on the complete chloroplast genome of Petrocodon longitubus 长舌石龙子完整叶绿体基因组的分子特征和系统发育定义

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-07-31 DOI: 10.1007/s11816-024-00919-z

Zaiqi Luo, FengXia Yan, Ronghui Jiang, Yanjun Chen, Changsha Luo, CongRui Li

{"title":"Molecular characteristics and phylogenetic definition on the complete chloroplast genome of Petrocodon longitubus","authors":"Zaiqi Luo, FengXia Yan, Ronghui Jiang, Yanjun Chen, Changsha Luo, CongRui Li","doi":"10.1007/s11816-024-00919-z","DOIUrl":"https://doi.org/10.1007/s11816-024-00919-z","url":null,"abstract":"Petrocodon is a small genus in the family Gesneriaceae, which is special for its remarkable floral diversity, and has high ornamental value. In this study, the complete chloroplast genome sequence and genome characteristics of Petrocodon longitubus are first reported. The genome size is 152,958 bp, including a large single-copy region (LSC, 83,901 bp), a small single-copy region (SSC, 18,255 bp), and two inverted repeat sequences (IRs, 25,401 bp, each). The chloroplast genome of P. longitubus was analyzed, revealing a total GC content of 37.47%. A total of 131 genes were de novo assembled, consisting of 87 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. A comparative analysis was conducted between the chloroplast genome of P. longitubus and three other published species of Petrocodon. The chloroplast genome of four Petrocodon species was found to have a double-chain ring structure, with a size ranging from 152,958 to 153,292 bp. Chloroplast genome size had indistinguishable. Four Petrocodon species was ra elatively conserved sequence, with 87 or 88 protein-coding genes, and 8 rRNA were the most conserved, which contains 42 ~ 50 SSR sites, which are mainly mononucleotides and dinucleotides, 4 boundary transition regions, then trinucleotides, pentanucleotides and hexanucleotides have been not detected. The non-preferred codons of the chloroplast genome in the four Petrocodon species are those ending in A, C, G, or T. The chloroplast genomes of these four Petrocodon species are highly similar to each other and to several Primulina species. Phylogenetic trees indicate that P. longitubus and other Petrocodon species were grouped together in a clade, with P. longitubus form a single clade. The results support the scientific naming of P. Longitubusr based on horticultural traits and further clarify the systematic status using molecular information.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141867976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enlightening the biotechnological approaches of legumes: present and past scenario to mitigate the biotic and abiotic stress 揭示豆科植物的生物技术方法：缓解生物和非生物胁迫的现状和前景

IF 2.4 4区生物学

Plant Biotechnology Reports Pub Date : 2024-07-24 DOI: 10.1007/s11816-024-00921-5

Janani Sree Sivakumar, Dhandapani Gurusamy, Selvakumar Subramaniam, Vasanth Krishnan

{"title":"Enlightening the biotechnological approaches of legumes: present and past scenario to mitigate the biotic and abiotic stress","authors":"Janani Sree Sivakumar, Dhandapani Gurusamy, Selvakumar Subramaniam, Vasanth Krishnan","doi":"10.1007/s11816-024-00921-5","DOIUrl":"https://doi.org/10.1007/s11816-024-00921-5","url":null,"abstract":"Legumes have played an essential part as food and feed, but their productivity is inadequate to deliver the protein needs of a rapidly growing human population and livestock sectors. In numerous places around the world, legumes are the peculiar source of protein which essential for the human consumption. They are typically used as a supplement to other dietary proteins. As legumes have the richest protein source, the health experts and consumers demand increased rapidly. Simultaneously, the inhabitants of humans in the universe increased promptly. Meanwhile, fluctuation in biotic and also abiotic components is an extensive constraint for crop growth. It may provoke stress which results in the reduction of yield. However, the legume shows recalcitrant nature, these are the major root cause of the shortage of food. The biotechnology provides a solution to rectify all those issues and genetic transformation plays a consequential impact on enhancement of the legume productivity. This review clearly emphasizes the constraints of legume productivity, the strategy used, skill requirement and current advances in biotechnology for legume improvement.","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141778089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic activity assays and functional component profiling reveals coordinated defense response of mungbean against Cercospora canescens during leaf spot disease 酶活性测定和功能成分分析揭示了绿豆在叶斑病期间对 Cercospora canescens 的协调防御反应

IF 1.7 4区生物学

Plant Biotechnology Reports Pub Date : 2024-07-15 DOI: 10.1007/s11816-024-00918-0

Mohammad Irfan, Mohd. Ashraf Bhat, Uzma Rashid, Enas A. Almanzalawi, Tahani M. Alqahtani, Sheikh Mansoor, Kyung-Hwan Boo

引用次数: 0

In planta production of human-derived RANKL 在植物体内生产源于人类的 RANKL

IF 1.7 4区生物学

Plant Biotechnology Reports Pub Date : 2024-07-15 DOI: 10.1007/s11816-024-00917-1

Jae-Ho Lee, Kyoung Rok Geem, Zhihao Chen, Jujin Jeong, Sang-Wook Park, Dong Wook Lee

引用次数: 0