甘薯抗逆性相关 GDP-L-半乳糖磷酸化酶基因 (IbGGP1) 对非生物胁迫反应的分子特性分析

IF 1.7 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yanxin Yang, Sijie Wang, Qirui Cheng, Xuan Zou, Zhe Yang, Peng Li, Yuan Wang, Dongjing Yang, Ho Soo Kim, Xiaoyun Jia, Lingzhi Li, Sang-Soo Kwak, Wenbin Wang
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引用次数: 0

摘要

抗坏血酸(AsA)可参与活性氧(ROS)的酶促和非酶促清除过程,从而提高植物的抗逆性。据预测,GDP-L-半乳糖磷酸化酶(GGP)是植物 AsA 生物合成过程中 L-半乳糖途径的关键酶。然而,有关甘薯中 GGP 基因催化 AsA 合成和抗逆作用的信息仍然很少。本研究成功分离了甘薯中的 IbGGP1 基因。通过qRT-PCR测定发现,IbGGP1在甘薯花中的表达水平明显较高,且该基因在多种胁迫下均有诱导作用,尤其是在干旱、盐渍和极端温度下。在甘露醇和盐胁迫下,与野生型植株相比,T3拟南芥IbGGP1表达株系的种子萌发率、根系伸长率和鲜重均有所提高。在土壤干旱和盐胁迫下,IbGGP1的异源过表达上调了AtGME和AtGPP基因的mRNA水平,并提高了AsA含量和AsA/DHA比值。这种抗逆表型与较低的过氧化氢和丙二醛含量以及较高的抗氧化酶活性有关。这些结果表明,拟南芥中 IbGGP1 表达量的增加可促进 AsA 的生物合成和 ROS 清除系统,从而提高对多种环境胁迫的耐受性。IbGGP1 的功能鉴定为提高甘薯和其他物种对干旱和盐胁迫的耐受性提供了一种新方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Molecular characterization of a sweetpotato stress tolerance-associated GDP-L-galactose phosphorylase gene (IbGGP1) in response to abiotic stress

Molecular characterization of a sweetpotato stress tolerance-associated GDP-L-galactose phosphorylase gene (IbGGP1) in response to abiotic stress

Ascorbic acid (AsA) can participate in the enzymatic and nonenzymatic clearance processes of reactive oxygen species (ROS), thereby enhancing stress tolerance in plants. GDP-L-galactose phosphorylase (GGP) is predicted to be a critical enzyme in the L-galactose route of plant AsA biosynthesis. However, information on the catalytic AsA synthesis and stress-resistance effect of the GGP gene in sweetpotato remains scarce. In this study, the IbGGP1 gene from sweetpotato was successfully isolated. The qRT-PCR determination revealed a distinctly higher expression level of IbGGP1 in sweetpotato flowers, and the gene was induced by multiple stresses, especially in drought, salt, and extreme temperatures. The seed germination, root elongation, and fresh weight were promoted in T3 Arabidopsis IbGGP1-overexpressing lines as compared to wild-type plants under mannitol and salt stresses. The heterologous overexpression of IbGGP1 upregulated the mRNA level of the AtGME and AtGPP genes, and elevated the AsA content and AsA/DHA ratio under soil drought and salt stress. This stress-tolerance phenotype was associated with lower hydrogen peroxide and malondialdehyde content and higher antioxidant enzyme activity. These results indicate that the increased expression of IbGGP1 in Arabidopsis improves tolerance to multiple environmental stresses by promoting AsA biosynthesis and ROS-scavenging system. The functional identification of IbGGP1 provides a new approach for improving stress tolerance to drought and salt in sweetpotato and other species.

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来源期刊
Plant Biotechnology Reports
Plant Biotechnology Reports 生物-生物工程与应用微生物
CiteScore
4.10
自引率
4.20%
发文量
72
审稿时长
>12 weeks
期刊介绍: Plant Biotechnology Reports publishes original, peer-reviewed articles dealing with all aspects of fundamental and applied research in the field of plant biotechnology, which includes molecular biology, genetics, biochemistry, cell and tissue culture, production of secondary metabolites, metabolic engineering, genomics, proteomics, and metabolomics. Plant Biotechnology Reports emphasizes studies on plants indigenous to the Asia-Pacific region and studies related to commercialization of plant biotechnology. Plant Biotechnology Reports does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.
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