Yanxin Yang, Sijie Wang, Qirui Cheng, Xuan Zou, Zhe Yang, Peng Li, Yuan Wang, Dongjing Yang, Ho Soo Kim, Xiaoyun Jia, Lingzhi Li, Sang-Soo Kwak, Wenbin Wang
{"title":"甘薯抗逆性相关 GDP-L-半乳糖磷酸化酶基因 (IbGGP1) 对非生物胁迫反应的分子特性分析","authors":"Yanxin Yang, Sijie Wang, Qirui Cheng, Xuan Zou, Zhe Yang, Peng Li, Yuan Wang, Dongjing Yang, Ho Soo Kim, Xiaoyun Jia, Lingzhi Li, Sang-Soo Kwak, Wenbin Wang","doi":"10.1007/s11816-024-00922-4","DOIUrl":null,"url":null,"abstract":"<p>Ascorbic acid (AsA) can participate in the enzymatic and nonenzymatic clearance processes of reactive oxygen species (ROS), thereby enhancing stress tolerance in plants. GDP-L-galactose phosphorylase (GGP) is predicted to be a critical enzyme in the L-galactose route of plant AsA biosynthesis. However, information on the catalytic AsA synthesis and stress-resistance effect of the <i>GGP</i> gene in sweetpotato remains scarce. In this study, the <i>IbGGP1</i> gene from sweetpotato was successfully isolated. The qRT-PCR determination revealed a distinctly higher expression level of <i>IbGGP1</i> in sweetpotato flowers, and the gene was induced by multiple stresses, especially in drought, salt, and extreme temperatures. The seed germination, root elongation, and fresh weight were promoted in T<sub>3</sub> <i>Arabidopsis IbGGP1</i>-overexpressing lines as compared to wild-type plants under mannitol and salt stresses. The heterologous overexpression of <i>IbGGP1</i> upregulated the mRNA level of the <i>AtGME</i> and <i>AtGPP</i> genes, and elevated the AsA content and AsA/DHA ratio under soil drought and salt stress. This stress-tolerance phenotype was associated with lower hydrogen peroxide and malondialdehyde content and higher antioxidant enzyme activity. These results indicate that the increased expression of <i>IbGGP1</i> in <i>Arabidopsis</i> improves tolerance to multiple environmental stresses by promoting AsA biosynthesis and ROS-scavenging system. The functional identification of <i>IbGGP1</i> provides a new approach for improving stress tolerance to drought and salt in sweetpotato and other species.</p>","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular characterization of a sweetpotato stress tolerance-associated GDP-L-galactose phosphorylase gene (IbGGP1) in response to abiotic stress\",\"authors\":\"Yanxin Yang, Sijie Wang, Qirui Cheng, Xuan Zou, Zhe Yang, Peng Li, Yuan Wang, Dongjing Yang, Ho Soo Kim, Xiaoyun Jia, Lingzhi Li, Sang-Soo Kwak, Wenbin Wang\",\"doi\":\"10.1007/s11816-024-00922-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Ascorbic acid (AsA) can participate in the enzymatic and nonenzymatic clearance processes of reactive oxygen species (ROS), thereby enhancing stress tolerance in plants. GDP-L-galactose phosphorylase (GGP) is predicted to be a critical enzyme in the L-galactose route of plant AsA biosynthesis. However, information on the catalytic AsA synthesis and stress-resistance effect of the <i>GGP</i> gene in sweetpotato remains scarce. In this study, the <i>IbGGP1</i> gene from sweetpotato was successfully isolated. The qRT-PCR determination revealed a distinctly higher expression level of <i>IbGGP1</i> in sweetpotato flowers, and the gene was induced by multiple stresses, especially in drought, salt, and extreme temperatures. The seed germination, root elongation, and fresh weight were promoted in T<sub>3</sub> <i>Arabidopsis IbGGP1</i>-overexpressing lines as compared to wild-type plants under mannitol and salt stresses. The heterologous overexpression of <i>IbGGP1</i> upregulated the mRNA level of the <i>AtGME</i> and <i>AtGPP</i> genes, and elevated the AsA content and AsA/DHA ratio under soil drought and salt stress. This stress-tolerance phenotype was associated with lower hydrogen peroxide and malondialdehyde content and higher antioxidant enzyme activity. These results indicate that the increased expression of <i>IbGGP1</i> in <i>Arabidopsis</i> improves tolerance to multiple environmental stresses by promoting AsA biosynthesis and ROS-scavenging system. The functional identification of <i>IbGGP1</i> provides a new approach for improving stress tolerance to drought and salt in sweetpotato and other species.</p>\",\"PeriodicalId\":20216,\"journal\":{\"name\":\"Plant Biotechnology Reports\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-08-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Biotechnology Reports\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s11816-024-00922-4\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Biotechnology Reports","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s11816-024-00922-4","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Molecular characterization of a sweetpotato stress tolerance-associated GDP-L-galactose phosphorylase gene (IbGGP1) in response to abiotic stress
Ascorbic acid (AsA) can participate in the enzymatic and nonenzymatic clearance processes of reactive oxygen species (ROS), thereby enhancing stress tolerance in plants. GDP-L-galactose phosphorylase (GGP) is predicted to be a critical enzyme in the L-galactose route of plant AsA biosynthesis. However, information on the catalytic AsA synthesis and stress-resistance effect of the GGP gene in sweetpotato remains scarce. In this study, the IbGGP1 gene from sweetpotato was successfully isolated. The qRT-PCR determination revealed a distinctly higher expression level of IbGGP1 in sweetpotato flowers, and the gene was induced by multiple stresses, especially in drought, salt, and extreme temperatures. The seed germination, root elongation, and fresh weight were promoted in T3Arabidopsis IbGGP1-overexpressing lines as compared to wild-type plants under mannitol and salt stresses. The heterologous overexpression of IbGGP1 upregulated the mRNA level of the AtGME and AtGPP genes, and elevated the AsA content and AsA/DHA ratio under soil drought and salt stress. This stress-tolerance phenotype was associated with lower hydrogen peroxide and malondialdehyde content and higher antioxidant enzyme activity. These results indicate that the increased expression of IbGGP1 in Arabidopsis improves tolerance to multiple environmental stresses by promoting AsA biosynthesis and ROS-scavenging system. The functional identification of IbGGP1 provides a new approach for improving stress tolerance to drought and salt in sweetpotato and other species.
期刊介绍:
Plant Biotechnology Reports publishes original, peer-reviewed articles dealing with all aspects of fundamental and applied research in the field of plant biotechnology, which includes molecular biology, genetics, biochemistry, cell and tissue culture, production of secondary metabolites, metabolic engineering, genomics, proteomics, and metabolomics. Plant Biotechnology Reports emphasizes studies on plants indigenous to the Asia-Pacific region and studies related to commercialization of plant biotechnology. Plant Biotechnology Reports does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.