大规模花生毛根培养基中高效花生素混合物的生物活性评估

IF 1.7 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Phadtraphorn Chayjarung, Kanjana Wongkrajang, Chanyanut Pankaew, Chonnikan Tothong, Wannakan Poonsap, Pakwuan Wongshaya, Siriwat Kucharoenphaibul, Anupan Kongbangkerd, Apinun Limmongkon
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引用次数: 0

摘要

花生毛根培养是生产有价值的前烯烃类化合物的有效工具。在生物反应器中使用大量壳聚糖(CHT)、茉莉酸甲酯(MeJA)和甲基-β-环糊精(CD)诱导毛细根,然后对其进行冷冻干燥并用丙酮提取。从第一柱色谱中获得的由部分纯化的混合花生苷组成的 Gr.4 组显示出最高的抗氧化活性,其粗提取物的抗氧化活性为 3,067.53 ± 176.98 µmole Trolox/克。此外,在检测抗菌活性时,部分纯化的 Gr.4 对金黄色葡萄球菌、鼠伤寒沙门氏菌、大肠杆菌和白色念珠菌的最小抑菌浓度 (MIC) 值最低,分别为 31、63、125 和 78 µg/ml。此外,使用芬顿反应进行的 DNA 挑刺试验表明,当质粒 DNA 暴露于浓度为 25-100 µg/ml 的部分纯化的 Gr.4 时,DNA 会受到损伤。此外,扫描电子显微镜(SEM)结果显示,在使用 2xMIC 的部分纯化的 Gr.4 处理后,所有受测微生物细胞都出现了不规则和异常现象。与粗提取物相比,混合花生素化合物表现出更强的活性,这表明混合花生素具有很高的效力。此外,分离和纯化的简易性表明,混合花生素化合物可作为有效化合物进一步应用,而不是以单独纯化的形式使用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Bioactivity assessment of high potency arachidin mixture from large-scale peanut hairy root culture medium

Bioactivity assessment of high potency arachidin mixture from large-scale peanut hairy root culture medium

Peanut hairy root culture serves as a potent tool for producing valuable prenylated stilbenoids. A significant amount of chitosan (CHT), methyl jasmonate (MeJA), and methyl-β-cyclodextrin (CD) was used to elicit hairy roots in a bioreactor, which were later subjected to freeze-drying and extracted with acetone. The group Gr.4, obtained from the first-column chromatography and consisting of partially purified mixed arachidin, exhibited the highest antioxidant activity, measuring 3,067.53 ± 176.98 µmole Trolox/g crude extract. Moreover, when examining the antimicrobial activity, the partially purified Gr.4 showed the lowest minimum inhibition concentration (MIC) values against Staphylococcus aureus, Salmonella Typhimurium, Escherichia coli, and Candida albicans, with values of 31, 63, 125, and 78 µg/ml, respectively. Furthermore, the DNA nicking assay using the Fenton reaction demonstrated the DNA damage effect when plasmid DNA was exposed to concentrations of 25–100 µg/ml of partially purified Gr.4. Additionally, the scanning electron microscopy (SEM) results revealed irregularities and abnormalities in all tested microbial cells after treatment with 2xMIC of partially purified Gr.4. The enhanced activity exhibited by mixed arachidin compounds compared to the crude extract indicates a high potency of mixed arachidin. Moreover, the simplicity of isolation and purification suggests that the mixed arachidin compounds could be a preferable alternative for further applications as effective compounds rather than using them in individually purified forms.

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来源期刊
Plant Biotechnology Reports
Plant Biotechnology Reports 生物-生物工程与应用微生物
CiteScore
4.10
自引率
4.20%
发文量
72
审稿时长
>12 weeks
期刊介绍: Plant Biotechnology Reports publishes original, peer-reviewed articles dealing with all aspects of fundamental and applied research in the field of plant biotechnology, which includes molecular biology, genetics, biochemistry, cell and tissue culture, production of secondary metabolites, metabolic engineering, genomics, proteomics, and metabolomics. Plant Biotechnology Reports emphasizes studies on plants indigenous to the Asia-Pacific region and studies related to commercialization of plant biotechnology. Plant Biotechnology Reports does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.
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