PLoS ONEPub Date : 2025-05-29eCollection Date: 2025-01-01DOI: 10.1371/journal.pone.0325842
Tao Feng, Xiaohuan Dong, Yueyun Wang
{"title":"Correction: Assessing the impact of digital transformation on capital market information efficiency under environmental uncertainty: Evidence from China.","authors":"Tao Feng, Xiaohuan Dong, Yueyun Wang","doi":"10.1371/journal.pone.0325842","DOIUrl":"https://doi.org/10.1371/journal.pone.0325842","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1371/journal.pone.0295187.].</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0325842"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Understanding public trust in information about interim nuclear waste storage: The roles of acceptance, gender, and proximity.","authors":"Aizhan Zabirova, Hitomi Matsunaga, Yuya Kashiwazaki, Xu Xiao, Thierry Schneider, Noboru Takamura","doi":"10.1371/journal.pone.0323896","DOIUrl":"https://doi.org/10.1371/journal.pone.0323896","url":null,"abstract":"<p><p>Following the accident at the Fukushima Daiichi Nuclear Power Station in March 2011, the Japanese government implemented extensive decontamination and waste management measures, including the establishment of an interim storage facility in Okuma and Futaba. This study aims to analyze residents' trust in the information provided by the public authorities regarding this facility and to identify factors influencing that trust. A survey was conducted among 1,558 former residents of Tomioka, Okuma, and Futaba who were 18 years or older and held resident cards for one of these towns in both March 2011 and 2024. The questionnaire collected data on demographic variables; intention to return; thoughts on the storage facility; trust in public information; concerns about radiation-related genetic risks and negative perceptions due to the nuclear accident; and mental health status, assessed using the mental component of the SF-8 health survey. The results showed that 57.7% of respondents trusted the information provided by the public authorities regarding the storage facility. Factors significantly associated with trust included their acceptance of the facility, lack of concerns about radiation-related genetic risks, lack of concerns about negative images resulting from the nuclear accident, and good mental health status. A logistic regression analysis indicated that acceptance of the facility and good mental health significantly increased the likelihood of trusting information provided by the public authorities, while concerns about genetic risks and negative images significantly decreased it. These findings underscore the vital importance of recognizing and addressing residents' concerns about health risks and negative perceptions related to nuclear waste management. By supporting the mental well-being of the community and fostering transparent communication, authorities can build trust and improve the acceptance of waste management facilities. Actively engaging with residents and responding to their worries facilitates the identification of adequate and acceptable recovery efforts after a nuclear accident.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0323896"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correlations between pain, functioning, and quality of life in manual wheelchair users with spinal cord injury.","authors":"Mokgadi Kholofelo Mashola, Elzette Korkie, Diphale Joyce Mothabeng","doi":"10.1371/journal.pone.0324850","DOIUrl":"https://doi.org/10.1371/journal.pone.0324850","url":null,"abstract":"<p><strong>Background: </strong>Pain after spinal cord injury (SCI) is one of the most important contributors to poor rehabilitation outcomes, reduced quality of life (QOL) as well as poorer physical, social, and psychological functioning.</p><p><strong>Objective: </strong>To determine the correlation of overall and shoulder pain on functioning and QOL in community-dwelling people with SCI.</p><p><strong>Methods: </strong>This quantitative correlational study included people with SCI with or without pain, who were discharged from five rehabilitation hospitals in Gauteng, South Africa. The presence of pain, wheelchair function, and QOL were investigated using the Numeric Rating Scale, Wheelchair Function Test Questionnaire, and the WHOQOL-BREF questionnaire. Pectoralis minor muscle (PMm) length was measured using a Vernier caliper and the Scapular Dyskinesis test was used to observe for scapular dyskinesis. Descriptive statistics; Independent t-tests, ANOVA tests, and Fisher's exact tests were performed using the SPSS v27 at a 0.05 level of significance.</p><p><strong>Results: </strong>85% of the 122 participants reported overall pain, mainly burning (32.7%) and below the level of injury (39.4%), with only 14.8% reporting shoulder pain. There was no overall difference in QOL between participants with and without pain, however, pain prevented individuals from doing what they needed to do (p < 0.05). Pain severity was negatively correlated with QOL (p < 0.001) and sleep satisfaction (p < 0.05). There were no correlations found between shoulder pain and PMm length, as well as scapular dyskinesis. However, there was a negative correlation between shoulder pain and wheelchair function in those who reported shoulder pain (p < 0.01).</p><p><strong>Conclusion: </strong>Pain is problematic after SCI and although shoulder pain is not as prominent, it has the potential to negatively impact an individual's ability to use their wheelchair effectively.</p><p><strong>Contribution: </strong>Findings from this study emphasise the negative role of pain on life satisfaction and QOL. Stakeholders involved in SCI rehabilitation can consider including comprehensive pain management within the interprofessional model of care.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0324850"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Spatial interpolation of health and demographic variables: Predicting malaria indicators with and without covariates.","authors":"Camille Morlighem, Chibuzor Christopher Nnanatu, Corentin Visée, Atoumane Fall, Catherine Linard","doi":"10.1371/journal.pone.0322819","DOIUrl":"https://doi.org/10.1371/journal.pone.0322819","url":null,"abstract":"<p><p>Accurate mapping and disaggregation of key health and demographic risk factors have become increasingly important for disease surveillance, which can reveal geographical social inequalities for improved health interventions and for monitoring progress on relevant Sustainable Development Goals (SDGs). Household surveys like the Demographic and Health Surveys have been widely used as a proxy for mapping SDG-related household characteristics. However, there is no consensus on the workflow to be used, and different methods have been implemented with varying complexities. This study aims to compare multiple modelling frameworks to model indicators of human vulnerability to malaria (SDG Target 3.3) in Senegal. These indicators were categorised into socioeconomic (e.g., stunting prevalence, wealth index) and malaria prevention indicators (e.g., indoor residual spraying, insecticide-treated net ownership). We compared three categories of the commonly used methods: (1) spatial interpolation methods (i.e., inverse distance weighting, thin plate splines, kriging), (2) ensemble methods (i.e., random forest), and (3) Bayesian geostatistical models. Most indicators could be modelled with medium to high predictive accuracy, with R2 values ranging from 0.40 to 0.86. No method or method category emerged as the best, but performance varied widely. Overall, socioeconomic indicators were generally better predicted by covariate-based models (e.g., random forest and Bayesian models), while methods using spatial autocorrelation alone (e.g., thin plate splines) performed better for variables with heterogeneous spatial structure, such as ethnicity and malaria prevention indicators. Increasing the complexity of the models did not always improve predictive performance, e.g., thin plate splines sometimes outperformed random forest or Bayesian geostatistical models. Beyond performance, we compared the different methods using other criteria (e.g., the ability to constrain the prediction range or to quantify prediction uncertainty) and discussed their implications for selecting a modelling approach tailored to the needs of the end user.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0322819"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PLoS ONEPub Date : 2025-05-29eCollection Date: 2025-01-01DOI: 10.1371/journal.pone.0322800
Yunting Wu, Aimin Zhu, Lijuan Yu, Wenbo Wang
{"title":"A study on fresh product supply chain management decisions considering subsidies and different transaction contracts.","authors":"Yunting Wu, Aimin Zhu, Lijuan Yu, Wenbo Wang","doi":"10.1371/journal.pone.0322800","DOIUrl":"https://doi.org/10.1371/journal.pone.0322800","url":null,"abstract":"<p><p>With the continuous improvement of people's living standards and the increasing demand for high-quality fresh products, enhancing the quality of fresh products has become an urgent and crucial issue that requires attention. Existing studies have shown that strengthening the preservation capacity of fresh products at the origin can effectively improve product quality. Therefore, this paper starts from the perspective of enhancing the freshness preservation level at the origin, considering providing financial subsidies to the supplier from both the government and the retailer's side, introducing the factor of supplier risk aversion behavior, and respectively constructing and solving the Stackelberg game models under wholesale price contract, cost-sharing contract, revenue-sharing contract, and transfer payment contract to explore how government subsidies and risk aversion affect the optimal decisions of members, the freshness of products, and the utility level of the supply chain. The research results indicate that: (1) The increase in government subsidies has a positive impact on the operation of the fresh product supply chain, which will raise wholesale prices, freshness preservation efforts, selling prices, and the overall utility level of the supply chain. (2) The risk aversion of supplier leads to a reduction in transfer payment costs, wholesale prices, and freshness preservation efforts, but this will, in turn, prompt retailer to lower selling prices, thereby enhancing the utility levels of both the retailer and the supply chain entity. (3) The freshness preservation effort by the supplier is higher in transfer payment contracts and cost-sharing contracts with a high cost-sharing ratio. Under transfer payment contracts, supplier and supply chain entity achieve the highest utility levels, while the most efficient utility level of retailer depends on the risk aversion threshold of supplier. When this threshold is exceeded, it will switch to cost-sharing contracts.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0322800"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PLoS ONEPub Date : 2025-05-29eCollection Date: 2025-01-01DOI: 10.1371/journal.pone.0325208
Jan Siefert, Niklas Zimmermann, Kandarp Thakkar, Ágnes Mészáros
{"title":"Pharmacy customers' views towards the potential introduction of pharmacist prescribing: A survey study.","authors":"Jan Siefert, Niklas Zimmermann, Kandarp Thakkar, Ágnes Mészáros","doi":"10.1371/journal.pone.0325208","DOIUrl":"https://doi.org/10.1371/journal.pone.0325208","url":null,"abstract":"<p><strong>Background: </strong>Pharmacist prescribing has been introduced in several countries owing to systemic issues within healthcare. This systematic change can improve general public's access to prescribed medication. In Germany, pharmacist prescribing and its acceptance among the population remains unexplored because legislators insist on the exclusive right of doctors to prescribe medication.</p><p><strong>Objectives: </strong>This study investigated pharmacy customers' views towards pharmacist prescribing. The study sought to determine whether pharmacy customers in Germany could imagine the introduction of pharmacist prescribing.</p><p><strong>Methods: </strong>Pharmacy customers in Germany participated in an anonymised survey in August-October 2023. Participants were recruited using a convenience sampling method, mainly from community pharmacies. The questionnaire comprised 28 questions and covers participants' characteristics, key aspects of patient care and statements regarding the topic of pharmacists prescribing. The collected data were coded and analysed using IBM SPSS version 28.0.1.</p><p><strong>Results: </strong>In total, 316 participants completed the survey. The average age was 51.0 years, and the majority were females (69.6%, n = 220). Overall, participants reported a high level of satisfaction with the general services provided by community pharmacies. A high to very high level of trust in their community pharmacist (CP) was reported by 84.4% of the participants. A total of 79.1% of the participants stated that pharmacists should be trained for prescribing authorisations. A key finding of this research was that most participants (88.0%), regardless of age and gender, agreed that the concept of pharmacist prescribing is favourable.</p><p><strong>Conclusion: </strong>Participants across all age groups were generally positive about pharmacist prescribing and supportive of its implementation, while expressing the need for pharmacists to undergo further training tailored to the specific prescribing authorisation. This study provides groundwork for further research and contributes to policy-making considerations.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0325208"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PLoS ONEPub Date : 2025-05-29eCollection Date: 2025-01-01DOI: 10.1371/journal.pone.0325043
Haley Q Marcarian, Anutr Sivakoses, Anika M Arias, Olivia C Ihedioha, Benjamin R Lee, Maria C Bishop, Alfred L M Bothwell
{"title":"Renal cancer cells acquire immune surface protein through trogocytosis and horizontal gene transfer.","authors":"Haley Q Marcarian, Anutr Sivakoses, Anika M Arias, Olivia C Ihedioha, Benjamin R Lee, Maria C Bishop, Alfred L M Bothwell","doi":"10.1371/journal.pone.0325043","DOIUrl":"https://doi.org/10.1371/journal.pone.0325043","url":null,"abstract":"<p><p>Trogocytosis is an underappreciated phenomenon that shapes the immune microenvironment surrounding many types of solid tumors. The consequences of membrane-bound proteins being deposited from a donor immune cell to a recipient cancer cell via trogocytosis are still unclear. Here, we report that human clear cell renal carcinoma tumors stably express the lymphoid markers CD45, CD56, CD14, and CD16. Flow cytometry performed on fresh kidney tumors revealed consistent CD45 expression on tumor cells, as well as varying levels of the other markers mentioned previously. These results were consistent with our immunofluorescent analysis, which also revealed colocalization of lymphoid markers with carbonic anhydrase 9, a standard kidney tumor marker. RNA analysis showed a significant upregulation of genes typically associated with immune cells by tumor cells. Finally, we show evidence of chromosomal DNA being transferred from immune cells to tumor cells through physical contact. This horizontal gene transfer has transcriptional consequences in the recipient tumor cell, resulting in a fusion phenotype that expresses both immune and cancer specific proteins. This work demonstrates a novel mechanism by which tumor cell protein expression is altered through the acquisition of surface membrane fragments and genomic DNA from infiltrating lymphocytes. These results alter the way in which we understand tumor-immune cell interactions and may reveal new insights into the mechanisms by which tumors develop. Additionally, further studies into trogocytosis and other mechanisms of contact-mediated cellular transfer will help push the field towards the next generation of immunotherapies and biomarkers for treating renal cell carcinoma and other cancers.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0325043"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Heat shock transcription factor 2 reduces mitochondrial pathway apoptosis in intestinal epithelial cells by inhibiting the increase in mitochondrial membrane permeability in ulcerative colitis.","authors":"Wen Wang, Yunling Wen, Juan Luo, Yinglei Miao, Fengrui Zhang, Junkun Niu","doi":"10.1371/journal.pone.0325275","DOIUrl":"https://doi.org/10.1371/journal.pone.0325275","url":null,"abstract":"<p><p>The destruction of intestinal mucosal mechanical barrier homeostasis caused by excessive apoptosis of intestinal epithelial cells (IECs) is an important reason for the occurrence and development of ulcerative colitis (UC). The increase in mitochondrial membrane permeability caused by the opening of the mitochondrial membrane permeability transition pore (mPTP) is a key link in the initiation of mitochondrial pathway apoptosis. Our previous studies revealed that heat shock transcription factor 2 (HSF2), which is highly expressed in the intestinal mucosa of UC patients, can inhibit the expression of the cytochrome C (Cyto-C)/Caspase-9/Caspase-3 proteins in the mitochondrial pathway of apoptosis, but the regulatory mechanism is unknown. It has been reported that heat shock proteins regulated by heat shock transcription factors are closely related to mPTP opening. Therefore, we hypothesized that HSF2 affects mitochondrial pathway apoptosis in IECs by regulating mPTP opening. In this study, we altered the level of HSF2 in Caco-2 cells by lentivirus transfection to explore the changes in the mitochondrial membrane permeability of Caco-2 cells in an inflammatory environment. Subsequently, the mPTP agonist atractylorhizin (Atr) and inhibitor cyclosporine A (CsA) were used to clarify the regulatory effects of HSF2 on mPTP and the Cyto-C/Caspase-9/Caspase-3 pathways. Our study confirmed for the first time that HSF2 plays a protective role in UC by inhibiting mPTP opening, the increase in mitochondrial membrane permeability and the activation of the mitochondrial-mediated apoptosis pathway in IECs.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0325275"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PLoS ONEPub Date : 2025-05-29eCollection Date: 2025-01-01DOI: 10.1371/journal.pone.0324465
Irene Simonetti, Stefano Landi, Chiara Leardini, Anna Giani, Arianna Bortolani, Francesco Fantin
{"title":"Impact of functional status and biomarkers on hospital costs and readmission rates in geriatric patients: An observational study with comprehensive geriatric assessment.","authors":"Irene Simonetti, Stefano Landi, Chiara Leardini, Anna Giani, Arianna Bortolani, Francesco Fantin","doi":"10.1371/journal.pone.0324465","DOIUrl":"https://doi.org/10.1371/journal.pone.0324465","url":null,"abstract":"<p><p>This study aimed to analyze the association between functional status, biomarkers, and hospitalization characteristics on costs and the probability of re-admission at 30 and 180 days in geriatric patients. It is used an observational design with both administrative data and additional clinical data not usually collected. Multivariate linear regression for hospitalization costs and multivariate logistic regressions for readmissions were used. Variables studied included the Barthel Index, Charlson index, albumin and blood pressure levels, previous hospitalizations, length of stay (LoS), and controls. Data from 953 patients aged over 65, admitted to the Geriatric ward between September 1st, 2018, and December 31st, 2019, were analyzed. The Charlson comorbidity index, number of comorbidities, and LoS were positively related to hospitalization costs. Previous hospitalizations and LoS were the main predictors of readmission. Systolic blood pressure was negatively associated with the odds of re-admission but showed no association with hospital costs. Higher functional status, as measured by the Barthel index, was linked to lower odds of unplanned hospitalization but was not statistically significant for costs. Functional status and biomarkers had moderate effects on costs and readmission odds. These findings can aid in early healthcare planning and resource management, providing valuable information for prioritizing patients and designing cost-effective care interventions.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0324465"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PLoS ONEPub Date : 2025-05-29eCollection Date: 2025-01-01DOI: 10.1371/journal.pone.0325016
Yang-In Yim, Xufeng Wu, Anjelika Gasilina, John A Hammer
{"title":"Two cancer cell lines utilize Myosin 10 and the kinesin HSET differentially to maintain mitotic spindle bipolarity.","authors":"Yang-In Yim, Xufeng Wu, Anjelika Gasilina, John A Hammer","doi":"10.1371/journal.pone.0325016","DOIUrl":"https://doi.org/10.1371/journal.pone.0325016","url":null,"abstract":"<p><p>Cancer cells often undergo mitosis possessing more than two centrosomes. To avoid a multipolar mitosis, the consequences of which are typically aneuploidy induced senescence, they must cluster their extra centrosomes to create a pseudo-bipolar spindle. Such supernumerary centrosome clustering (SNCC) requires Myosin 10 (Myo10) and the pole-focusing kinesin HSET. We showed recently that Myo10 promotes SNCC in HeLa cells by promoting retraction fiber-based cell adhesion, and that it further supports spindle bipolarity by preventing the generation of extra spindle poles via pericentriolar material (PCM) fragmentation. Here we quantified the contribution that Myo10 and HSET make individually and together to SNCC and PCM/pole integrity in HeLa cells and in MDA-MB-231 cells, which differ from HeLa in being more dependent on SNCC and less dependent on retraction fiber-based cell adhesion. As expected, knockdown of Myo10 and HSET individually increased the frequency of multipolar spindles in both cell types. Their effects were surprisingly not additive, however. For HeLa and MDA-MB-231 cells undergoing mitosis with more than two centrosomes, the defect in SNCC was almost entirely responsible for their multipolar phenotype following knockdown of either Myo10 or HSET. For HeLa and MDA-MB-231 cells undergoing mitosis with two centrosomes, PCM/pole fragmentation was the primary cause of multipolar spindles following HSET knockdown. Unlike HeLa, however, MDA-MB-231 cells exhibited very little PCM/pole fragmentation following Myo10 knockdown. This difference may be due to the smaller role that Myo10 plays in retraction fiber-based adhesion in MDA-MB-231. Finally, we show that HSET knockdown disrupts retraction fiber formation and organization, which may explain why the defects in double knockdown cells were not significantly greater than in HSET knockdown cells. These and other results can inform efforts to target these two motor proteins to selectively kill cancer cells by increasing their frequency of multipolar divisions.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0325016"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}