PLoS ONE最新文献

{"title":"In vivo quantification of collagen-induced arthritis mouse model by three-dimensional volumetric ultrasound and color Doppler.","authors":"Qingyun Jia, Hao Xu, Tengteng Wang, Qianqian Liang, Lisheng Wu","doi":"10.1371/journal.pone.0321124","DOIUrl":"10.1371/journal.pone.0321124","url":null,"abstract":"<p><strong>Purpose: </strong>Ultrasound combined with Doppler techniques has been widely utilized to evaluate joint inflammation and internal structural changes in animal models of rheumatoid arthritis. However, previous assessments using these techniques were predominantly semi-quantitative, which may limit the precision and reliability of the results. Therefore, the primary objective of this study was to explore the potential advantages of three-dimensional (3D) volumetric ultrasound and color Doppler in quantitatively assessing the progression of disease.</p><p><strong>Objective: </strong>To quantify the severity and progression of collagen-induced arthritis (CIA) mouse model using a micro-imaging tool, 3D volumetric ultrasound and color Doppler, and assess if 3D model-based volumetric changes of joint space and vascularity correlate with clinical, histological and bone destruction findings.</p><p><strong>Methods: </strong>CIA was induced in mice on a DBA/1J background at 7 ~ 8 weeks of age. 3D volumetric ultrasound and color Doppler analysis was performed on knee and ankle joints of all mice using the Vevo 2100 system at 0, 2, 4, 8 weeks after the booster immunization. Clinical, histological and bone destruction analysis were performed as usual. Correlation analysis of the volumetric changes of joint space and vascularity with clinical, histological and bone destruction score were assessed via Spearman's test.</p><p><strong>Results: </strong>It was possible to quantify the severity of joint inflammation and intra-articular changes during the progression of CIA by 3D volumetric ultrasound and color Doppler. The 3D model-based volumetric changes of joint space and vascularity have significant correlations with clinical, histological and bone destruction score of arthritis.</p><p><strong>Conclusions: </strong>We demonstrated that 3D volumetric ultrasound and color Doppler is a noninvasive, quantitative tool for evaluating CIA mice in vivo. Despite certain limitations, those technology significantly enhance our ability to monitor disease progression and severity, assess therapeutic interventions, and reduce reliance on invasive techniques.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 4","pages":"e0321124"},"PeriodicalIF":2.9,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring physical behavior in pediatric physical therapy: A mixed methods feasibility study to evaluate a newly developed toolkit and training.","authors":"Barbara Engels, Elles Kotte, Raoul Engelbert, Marleen E Sol, Remko van der Lugt, Harriët Wittink, Jan Willem Gorter, Manon A T Bloemen","doi":"10.1371/journal.pone.0319659","DOIUrl":"10.1371/journal.pone.0319659","url":null,"abstract":"<p><strong>Introduction: </strong>Pediatric physical therapists (PPTs) aim to enhance active physical behavior but lack feasible accelerometry devices to assess and evaluate physical activity (PA). We developed an activity monitoring prototype toolkit (AM-p Toolkit) consisting of a wearable, a docking station, a digital tool for data analysis, and physical tools for communication with children and parents. A training for PPTs was also created. We aim to explore the feasibility of the AM-p Toolkit from the perspectives of PPTs, children, and parents and to assess if training improved PPTs' knowledge, skills, and confidence in using the Toolkit.</p><p><strong>Participants and methods: </strong>Using an explanatory sequential mixed methods design, we collected data through questionnaires, individual interviews, and focus groups, guided by Bowen's dimensions of 'acceptability,' 'demand,' and 'practicality.' We included children with the ability to walk, their parents, and their PPTs. The training was evaluated by analyzing PPTs' knowledge, skills, and confidence using the AM-p Toolkit. Quantitative results were analyzed descriptively (mean [SD] and median [interquartile range] when appropriate and qualitative data were analyzed thematically.</p><p><strong>Results: </strong>Fifteen PPTs, 17 parents, and 20 children completed the study. PPTs rated overall satisfaction on a 10-point scale with the AM-p Toolkit at 6.3 (SD 1.2), and parents rated it 7.3 (SD 1.6). The following themes emerged for acceptability, demand, and practicality respectively: for acceptability: 1) expected added value, 2) quality and usability, and 3) design; for demand: 1) use and non-use, 2) further development, and 3) willingness for future use; and for practicality: 1) time constraints and 2) integration.</p><p><strong>Conclusion: </strong>The AM-p Toolkit shows promise in PPT, with generally positive acceptability among all end-users. PPTs see potential for certain groups of children who can benefit from the AM-p Toolkit. Practicality requires improvements in the web application and refinement of the strap. Training is important and can be strengthened by emphasizing the analysis of assessment results, clinical reasoning, and functional goal-setting.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 4","pages":"e0319659"},"PeriodicalIF":2.9,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of essential tremor on longevity and mortality rates in families.","authors":"Onur Emre Onat, Faruk Ustunel, Cem Akbostanci, Kivilcim E Doganyigit, Merve Sen, Emre Can Gunaydin, Kaya Bilguvar, Muhittin Cenk Akbostanci","doi":"10.1371/journal.pone.0320422","DOIUrl":"10.1371/journal.pone.0320422","url":null,"abstract":"<p><p>Essential Tremor (ET) is a common movement disorder characterized by action tremors, primarily affecting the hands and head. lthough previous studies have suggested potential links between ET and aging-related diseases, its relationship with longevity remains unclear, with conflicting evidence in the literature. To investigate this association, we analyzed data from 1,493 individuals across 145 families, encompassing both ET-positive (ET+) and ET-negative (ET-) participants. Using comprehensive statistical methods, including survival function estimation and regression modeling, we examined the potential influence of ET on lifespan. The median age of our participants was 67 years (IQR 54-77). Among deceased individuals, those with ET had a higher median age at death (80 years, IQR 70-86) compared to their ET- counterparts (70 years, IQR 59-77). Living ET+ participants also demonstrated slightly higher median ages (63 years, IQR 53-74) than living ET- individuals (60 years, IQR 49-71). Survival analysis revealed a significantly prolonged lifespan for ET+ individuals compared to ET- individuals (log-rank p =  1.11 ×  10 ⁻23). Furthermore, hazard ratio (HR) calculations indicated a reduced risk of mortality for the ET+ group (HR =  0.44, CI95% =  0.37-0.52), particularly among males. These findings suggest that ET may be associated with increased longevity, though the underlying biological mechanisms remain unclear. Further research is essential to elucidate the processes contributing to this relationship and to explore its implications for understanding aging and neurodegenerative disorders.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 4","pages":"e0320422"},"PeriodicalIF":2.9,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel ex vivo protocol that mimics length and excitation changes of human muscles during walking induces force losses in EDL but not in soleus of mdx mice.","authors":"Xiao Hu, Allison N McCrady, Katherine E Bukovec, Claire Yuan, Emily Y Miller, Rachel K Bour, Anthony C Bruce, Katherine B Crump, Shayn M Peirce, Robert W Grange, Silvia S Blemker","doi":"10.1371/journal.pone.0320901","DOIUrl":"10.1371/journal.pone.0320901","url":null,"abstract":"<p><p>Although eccentric contraction protocols are widely used to study the pathophysiology and potential treatments for Duchenne muscular dystrophy (DMD), they do not reflect the stresses, strains, strain rates, and excitation profiles that DMD muscles experience during human daily functional tasks, like walking. This limitation of eccentric contractions may impede our understanding of disease progression in DMD and proper assessment of treatment efficacy. The goals of this study were to examine the extent of force loss induced by a gait cycling protocol we developed, and compare to that from a typical eccentric contraction protocol in soleus and extensor digitorum longus (EDL) muscles of mdx mice. To achieve this goal, mdx soleus and EDL muscles were subjected to eccentric contractions at three levels of strain (10%, 20% and 30% optimal length Lo) and up to 200 cycles of our gait cycling protocol that mimicked the length changes and excitation patterns of the corresponding muscles during human walking gait. Our results showed that EDL but not soleus muscles had significant losses in isometric tetanic forces after the cycling protocols. Compared to the eccentric contraction protocol, the decrements in contractile performance from the cycling protocol were similar to those from the eccentric contractions at 10% in soleus and 20% Lo in EDL. Together, these results indicated the gait cycling protocol is a valuable experimental approach to better understand disease progression and to screen and evaluate efficacy of novel therapeutics for DMD.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 4","pages":"e0320901"},"PeriodicalIF":2.9,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving HIV outcomes in Miami's Black populations with clinic-based community health workers protocol: The integrated navigation and support for treatment adherence, counseling, and research (INSTACARE) randomized controlled trial.","authors":"Sonjia Kenya, BreAnne Young, Sebastian Escarfuller, Deborah Jones-Weiss, Olveen Carrasquillo, Ana Riccio, Pan Yue","doi":"10.1371/journal.pone.0316300","DOIUrl":"10.1371/journal.pone.0316300","url":null,"abstract":"<p><strong>Trial registration: </strong>ClinicalTrials.gov NCT04663152.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 4","pages":"e0316300"},"PeriodicalIF":2.9,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of zinc conjugated with Doxorubicin for the prevention of aggregating β-catenin in the Wnt signaling pathway investigated through computational approaches.","authors":"Gomathi Rajagopal, Balajee Ramachandran, Paradesi Deivanayagam, Venkatesan Srinivasadesikan, Boomi Pandi, Saravanan Muthupandian, Rajamanikandan Sundarraj, Goyitom Gebremedhn Gebru","doi":"10.1371/journal.pone.0316665","DOIUrl":"10.1371/journal.pone.0316665","url":null,"abstract":"<p><p>Canonical Wnt signaling plays a key role in tumor cell proliferation which correlates with the accumulation of β-catenin resulting inactivation of the network of targets such as GSK3β, Axin, CK1. Uncontrolled expression of β-catenin leads to different types of cancers and other diseases such as sarcoma and mesenchymal tumor formation. However, β-catenin is an attractive target for cervical cancer. In the present study, the compounds such as Doxorubicin and Zinc conjugated with Doxorubicin were screened against β-catenin using Molecular Docking, Molecular Dynamics Simulation, MM/GBSA, and DFT approaches to explore their insights. The study further demonstrated that the binding energy of Zn conjugated with Doxorubicin has shown -7.2 kcal/mol and Doxorubicin registers -5.9 kcal/mol against β-catenin. The disruption between the β-catenin/Tcf-4 complex was observed through the Zinc-Doxorubicin complex, both the proteins are separated about 12 Å. The Zn-Doxorubicin was stabilized with the hydrophobic residues such as Val349 of β-catenin and Phe21 of Tcf-4. The DFT analysis using the B3LYP/6-31g(d,p) method explores that Zn-doxorubicin in complex with the binding site residues has shown the HOMO-LUMO gap of 2.55 eV. The binding free energy calculations exhibit the Zn conjugated Doxorubicin favors in the study by showing ~ 3 kcal/mol difference with Doxorubicin. The Zn-conjugated Doxorubicin will be discussed in the context of cervical cancer with the hope of improving drug efficacy and reducing toxicities for the betterment of the patient's quality of life.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 4","pages":"e0316665"},"PeriodicalIF":2.9,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nature-based interventions for individual, collective and planetary wellbeing: A protocol for a scoping review.","authors":"Jesse Blackburn, Afonso Pereira, Luke Jefferies, Andrew H Kemp, Amy Isham","doi":"10.1371/journal.pone.0314591","DOIUrl":"10.1371/journal.pone.0314591","url":null,"abstract":"<p><p>Nature-based interventions (NBIs) provide an opportunity to enhance individual wellbeing, improve community cohesion, and promote a culture of care for the environment. Several scoping reviews have attempted to catalogue the positive effects of NBIs on wellbeing, yet, these have typically focused on outcomes relating to individual wellbeing, thus restricting the assessment of the possible benefits of NBIs. Here we present a protocol for a scoping review that will synthesise the evidence relating to the impact of NBIs across a much broader range of domains with a focus on self (individual wellbeing), others (collective wellbeing) and nature (planetary wellbeing). This scoping review will also provide insight into the relative effectiveness of different types of NBIs at enhancing wellbeing across these domains and synthesise the underlying theory on which interventions have been developed and reported outcomes have been presented. A literature search for theses and peer-reviewed studies will be conducted on four databases (APAPsycINFO, Web of Science, Medline, and Scopus) and ProQuest Dissertations & Theses Global. Two independent reviewers will complete a two-stage screening process (title/abstract and full-text) using the Covidence platform. The protocol for this scoping review is registered with the Open Science Framework. Data extraction will focus on publication details, type of intervention, and wellbeing-related outcomes. Results will be reported in a scoping review following standardised guidelines relating to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews. This research will inform the design and delivery of NBI's across a wide range of sectors including health and social care, public policy, education, and community services, to ultimately promote human flourishing at scale.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 4","pages":"e0314591"},"PeriodicalIF":2.9,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ATF6β is not essential for the development of physiological cardiac hypertrophy.","authors":"Emery Davis, Mohammad-Reza Shokri, Mary B Rowland, Thomas York, Caroline T Cohen, Anna Grace Johnson, Patrick E Moore, Saige Schweitzer, Jon Sin, Chuong Bui, Robert N Correll","doi":"10.1371/journal.pone.0320178","DOIUrl":"10.1371/journal.pone.0320178","url":null,"abstract":"<p><p>Physiological cardiac hypertrophy is a compensatory remodeling of the heart in response to stimuli such as exercise training or pregnancy that is reversible and well-tolerated. We previously described how the activating transcription factor 6 (ATF6) proteins, ATF6α and ATF6β, were required for pathological hypertrophy in response to hemodynamic stress. Here, we examine the functional roles of both ATF6 proteins in the context of exercise-induced physiological hypertrophy. After 20 days of swim training, we found differential roles: whole body gene-deleted mice lacking ATF6α had an attenuated hypertrophic response compared to wild-type mice but those lacking ATF6β did not. Additionally, mice lacking ATF6α displayed ventricular dilation and reduced fractional shortening after swimming. While we observed no differences in the expression of downstream UPR signaling between the exercise groups, mice lacking ATF6α showed enhanced phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2). Thus, in response to swim training, loss of ATF6β did not hinder the development of physiological hypertrophy, but loss of ATF6α resulted in significantly reduced cardiac fractional shortening.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 4","pages":"e0320178"},"PeriodicalIF":2.9,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Online risk exposure and anxiety among college students in China: The chain mediating role of negative attribution and interpersonal security.","authors":"","doi":"10.1371/journal.pone.0322421","DOIUrl":"10.1371/journal.pone.0322421","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1371/journal.pone.0319700.].</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 4","pages":"e0322421"},"PeriodicalIF":2.9,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased preference for lysine over arginine in spike proteins of SARS-CoV-2 BA.2.86 variant and its daughter lineages.","authors":"Anže Božič, Rudolf Podgornik","doi":"10.1371/journal.pone.0320891","DOIUrl":"10.1371/journal.pone.0320891","url":null,"abstract":"<p><p>The COVID-19 pandemic offered an unprecedented glimpse into the evolution of its causative virus, SARS-CoV-2. It has been estimated that since its outbreak in late 2019, the virus has explored all possible alternatives in terms of missense mutations for all sites of its polypeptide chain. Spike protein of the virus exhibits the largest sequence variation in particular, with many individual mutations impacting target recognition, cellular entry, and endosomal escape of the virus. Moreover, recent studies unveiled a significant increase in the total charge on the spike protein during the evolution of the virus in the initial period of the pandemic. While this trend has recently come to a halt, we perform a sequence-based analysis of the spike protein of 2665 SARS-CoV-2 variants which shows that mutations in ionizable amino acids continue to occur with the newly emerging variants, with notable differences between lineages from different clades. What is more, we show that within mutations of amino acids which can acquire positive charge, the spike protein of SARS-CoV-2 exhibits a prominent preference for lysine residues over arginine residues. This lysine-to-arginine ratio increased at several points during spike protein evolution, most recently with BA.2.86 and its sublineages, including the recently dominant JN.1, KP.3, and XEC variants. The increased ratio is a consequence of mutations in different structural regions of the spike protein and is now among the highest among viral species in the Coronaviridae family. The impact of high lysine-to-arginine ratio in the spike proteins of BA.2.86 and its daughter lineages on viral fitness remains unclear; we discuss several potential mechanisms that could play a role and that can serve as a starting point for further studies.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 4","pages":"e0320891"},"PeriodicalIF":2.9,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}