PLoS ONE最新文献

{"title":"Acceptance-based process variables on postpartum well-being and distress: The mediating role of psychological flexibility.","authors":"Alexa Bonacquisti, Emily C Woodworth, Maria Diaz, Victoria A Grunberg","doi":"10.1371/journal.pone.0312454","DOIUrl":"https://doi.org/10.1371/journal.pone.0312454","url":null,"abstract":"<p><strong>Objective: </strong>Although up to 20% of women experience postpartum depression and/or anxiety, current interventions are limited. Identifying the processes that impact outcomes can inform and enhance interventions. Our study aims to examine: (1) whether acceptance-based process variables (awareness, acceptance, cognitive defusion, psychological flexibility) were associated with postpartum outcomes; and (2) whether psychological flexibility mediated the relationship between treatment engagement and postpartum outcomes.</p><p><strong>Method: </strong>Postpartum women (N = 266) were recruited online using snowball sampling via social media (i.e., Facebook). They completed a cross-sectional survey of validated and reliable quantitative measures assessing their well-being, emotional distress, and acceptance-based processes.</p><p><strong>Results: </strong>Hierarchical regressions revealed that women with more awareness (β = .13; p = .023), acceptance (β = .17; p = .036), and cognitive defusion (β = -.46; p < .001) reported better well-being. When adding in psychological flexibility, cognitive defusion (β = -.24; p = .031) and psychological flexibility (β = -.33; p = .003) explained variance in well-being. Women with more acceptance (β = -.32; p < .001) and cognitive defusion (β = .52; p < .001) endorsed less emotional distress. When adding in psychological flexibility, acceptance (β = -.28; p < .001), cognitive defusion (β = .40; p < .001), and psychological flexibility (β = .18; p = .047) explained variance in emotional distress. Mediation models revealed that psychological flexibility explained the relationship between mental health treatment and well-being (b = 3.91, SE = 0.66, CI = [2.69, 5.30]) and emotional distress (b = -5.74, SE = 1.08, CI = [-1.26, 3.00]).</p><p><strong>Conclusions: </strong>Targeting these acceptance-based processes in interventions may help to improve maternal, child, and family outcomes.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"19 12","pages":"e0312454"},"PeriodicalIF":2.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Binocular contributions to motion detection and motion discrimination during locomotion.","authors":"Hongyi Guo, Robert S Allison","doi":"10.1371/journal.pone.0315392","DOIUrl":"https://doi.org/10.1371/journal.pone.0315392","url":null,"abstract":"<p><p>During locomotion, the visual system can factor out the motion component caused by observer locomotion from the complex target flow vector to obtain the world-relative target motion. This process, which has been termed flow parsing, is known to be incomplete, but viewing with both eyes could potentially aid in this task. Binocular disparity and binocular summation could both improve performance when viewing with both eyes. To separate the binocular disparity and binocular summation and analyse how they affect flow parsing, we tested detection and discrimination thresholds under three viewing conditions: stereoscopic, synoptic (binocular but without disparity) and monocular. Experiment 1 tested motion detection during simulated forward self-motion and when stationary. Experiment 2 and 3 tested motion discrimination in forward and backward self-motion and stationary conditions. We found that binocular disparity significantly improved detection thresholds and discrimination biases, at the cost of lower precision. Binocular summation only significantly improved detection thresholds when stationary. It did not significantly affect detection thresholds during locomotion, discrimination biases, or discrimination precisions. Our results indicated that both binocular summation and binocular disparity contribute to motion detection and motion discrimination, but they affect performance differently while stationary and during locomotion.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"19 12","pages":"e0315392"},"PeriodicalIF":2.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of lysine and methionine on mRNA expression of candidate transcription factors by primary bovine mammary epithelial cells.","authors":"Boning Li, Ashlin M Edick, Madison K Fox, John Doelman, Sergio A Burgos, John P Cant","doi":"10.1371/journal.pone.0305440","DOIUrl":"https://doi.org/10.1371/journal.pone.0305440","url":null,"abstract":"<p><p>It has been established that essential amino acids (EAA) regulate protein synthesis in mammary epithelial cells by rapidly altering the phosphorylation state of translation factors. However, the long-term transcriptional response to EAA supply has been investigated much less. Eight transcription factors were selected as candidate mediators of EAA effects on mammary cell function via the amino acid response (ATF4, ATF6), mitogen-activated protein kinase (JUN, FOS, EGR1), and mechanistic target of rapamycin complex 1 (MYC, HIF1A, SREBF1). The objective was to determine if and when expression of these candidate genes was affected in primary cultures of bovine mammary epithelial cells more than 24 h after imposing an EAA deficiency, and to evaluate effects of EAA deficiency on protein synthesis, endoplasmic reticulum size, cell proliferation, and lipogenesis. Differentiated cells were cultured in 1 of 3 treatment media representing normal physiological concentrations of all amino acids (CTL), low lysine (LK), or low methionine (LM) for 24, 40, 48, or 60 h. Both LK and LM suppressed protein synthesis and activated ATF4 expression, indicating the classic amino acid response pathway had been triggered. However, there was no effect of LK or LM on endoplasmic reticulum size, possibly related to elevated ATF6 expression on LM. Expression of early response genes JUN, FOS, EGR1 and MYC was not elevated by EAA deficiency but LM decreased EGR1 expression. LM also increased expression of HIF1A. The EGR1 and HIF1A expression results are consistent with the decrease in cell proliferation rate observed. Variable responses in SREBF1 expression to LK and LM at different timepoints may have contributed to a lack of effect on lipogenesis rates. These findings indicate that EAA deficiency may inhibit mammary protein synthesis and cell proliferation through transcription factors.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"19 12","pages":"e0305440"},"PeriodicalIF":2.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revolutionizing cross-border e-commerce: A deep dive into AI and big data-driven innovations for the straw hat industry.","authors":"Junjie Dai, Xiaoyan Mao, Pengyue Wu, Huijie Zhou, Lei Cao","doi":"10.1371/journal.pone.0305639","DOIUrl":"https://doi.org/10.1371/journal.pone.0305639","url":null,"abstract":"<p><p>This paper investigates the impact of artificial intelligence (AI) and big data analytics on optimizing cross-border e-commerce efficiency for straw hat manufacturers in Zhejiang Province, China. It identifies market and consumer demand trends through machine learning analysis of comprehensive e-commerce data and leverages generative AI to revolutionize production and marketing processes. The integration of AI-generated content (AIGC) technology facilitates streamlined design-to-production cycles and rapid adaptation to market changes and consumer feedback. Findings demonstrate that the application of AI and big data significantly enhances market responsiveness and sales performance for straw hat enterprises in cross-border e-commerce. This research contributes a novel framework for employing AI and big data to navigate the complexities of international commerce, providing strategic insights for small and micro enterprises seeking to expand their global market footprint.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"19 12","pages":"e0305639"},"PeriodicalIF":2.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of internal coupling and coordination degree and diagnosis of obstacle factors for high-quality regional economic development: Evidence from Chongqing's \" One District, Two Groups\".","authors":"Sifang Che, Xi Zhang, Weijia Shu","doi":"10.1371/journal.pone.0312820","DOIUrl":"https://doi.org/10.1371/journal.pone.0312820","url":null,"abstract":"<p><p>The study builds a high-quality development index system encompassing dimensions of economic vitality, coordinated development, green development, and digital development. Utilizing the entropy weight TOPSIS method, the coupling coordination model, and the barrier factor model, this research examines the high-quality economic development level, internal coupling coordination, and obstacle factors in Chongqing's \"one district and two groups\" from 2017 to 2021. The findings are as follows: (1)The high-quality economic development level of the \"one district and two groups\" has been increasing year by year. This growth is driven by the joint progress across all dimensions, though regional disparities exist in the development levels of different dimensions. The order of high-quality economic development from highest to lowest is: the main city metropolitan area, southeast Chongqing, and northeast Chongqing. (2)The development level of internal coupling coordination for high-quality economic development within the \"one district and two groups\" has also been rising annually. However, the overall level of coupling coordination development remains low, although regional coupling coordinated development is gradually forming. There was no evidence of σ convergence in the coupling coordination of high-quality economic development within the \"one district and two groups.\" The main city metropolitan area shows stability with an expansion trend, while the \"two groups\" exhibit divergence. (3)Overall, Chongqing's barrier index order was \"economic vitality > coordinated development > green development > digital development.\" There were differences in obstacle factors between \"one district\" and \"two groups\"; the \"two groups\" align with the overall performance, while the main metropolitan area performs as \"green development > economic vitality > coordinated development > digital development.\" The research conclusions provide a theoretical basis and decision-making reference for improving coordinated development within the region. (4)The main obstacles to high-quality economic development in Chongqing and the \"one district and two groups\" are centered on the dimensions of economic vitality and coordinated development, while also reflecting green development considerations.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"19 12","pages":"e0312820"},"PeriodicalIF":2.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cannabis sativa extracts inhibit LDL oxidation and the formation of foam cells in vitro, acting as potential multi-step inhibitors of atherosclerosis development.","authors":"Bruno Musetti, Alejandra Kun, David Menchaca, Alejandra Rodríguez-Haralambides, Javier Varela, Leonor Thomson, Edward M Bahnson","doi":"10.1371/journal.pone.0310777","DOIUrl":"https://doi.org/10.1371/journal.pone.0310777","url":null,"abstract":"<p><p>Atherosclerotic disease is the leading cause of death world-wide. Our goal was to explore the effect of phytocannabinoids on the molecular mechanisms triggering the development of the atheromatous lesion. Three cannabis sativa extracts of different chemotypes were chemically characterized by UPLC-DAD. The capacity of the extracts to prevent the oxidation of LDL, the formation of foam cells and the activation of an inflammatory response by J774 cells, were monitored by UV-Vis spectrometry, confocal-microscopy and western blot. Three varieties of cannabis sativa, with high (E1), intermediate (E2) and low (E3) THC/CBD ratios were selected. The three cannabis extracts inhibited the oxidation of LDL by copper ions and the formation of foam cells by J774.1 cells challenged with oxLDL (ED50 5-12 μg mL-1). The effect of the cannabinoid extracts on the endocytic process was independent of the canonical cannabinoid receptors, CB1 and CB2, but related to the action of non-canonical receptors (TRPV1, TRPV4 and GPR55), involved in calcium signaling. Decreased levels of CD36 and OLR1 scavenger receptors were, at least partially, responsible for the diminished uptake of oxLDL induced by phytocannabinoids. The downregulation of CD36 and OLR1 could be explained by the observed inhibitory effect of the cannabis extracts on the activation of the NFκB pathway by oxLDL. Phytocannabinoids interfere with the main events leading to the development of the atheromatous plaque, opening new venues on atherosclerosis therapy.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"19 12","pages":"e0310777"},"PeriodicalIF":2.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pattern, causes and functional outcome of peripheral neuropathies in the Amazon region.","authors":"Nathalie Deschamps, Mathieu Nacher, Pierre-Marie Preux, Valérie Takam, Romain Blaizot, Beatrice Cenciu, Nadia Sabbah, Bertrand De Toffol","doi":"10.1371/journal.pone.0315760","DOIUrl":"https://doi.org/10.1371/journal.pone.0315760","url":null,"abstract":"<p><strong>Background: </strong>Peripheral neuropathies have a wide variety of causes and pathophysiologies. Assuming that there are local particularities in the Amazon region, the aim of this study was to describe the characteristics of patients by determining the factors associated with functional severity.</p><p><strong>Methods: </strong>This was a retrospective observational study at Cayenne Hospital between January 2015 and May 2023. We included patients from the French hospital activity monitoring database \"Programme de médicalisation des systèmes d'information\" by searching for the keywords \"neuropathy\" \"Guillain-Barre syndrome\"(GBS) \"multineuritis\" \"polyneuritis\". The Peripheral Neuropathy Disability (PND) score was determined to quantify the functional severity of patients.</p><p><strong>Results: </strong>A total of 754 patients were included, with a mean age of 60 years (SD = 14.6) and a predominance of women (53.6%). Gait disorders were present in 16.3% of patients (PND between 2 and 4). Mortality was 14.3% at 3 years. The most common causes of neuropathy were diabetes (58.2%), chemotherapy induced polyneuropathy toxicity (21.5%), Guillain-Barre syndrome (6.0%), unlabeled (3.2%), and infectious causes (2.0%). Infectious causes mainly included HIV in 13 patients (43.3%) and leprosy in 8 patients (26.7%). Only GBS was significantly associated with functional severity but all patients with a deficiency had a severe score. But, after excluding GBS, infectious causes were significantly associated with a severe PND score (aOR = 3.69 [1.18-11.58]).</p><p><strong>Discussion: </strong>The characteristics of French Guiana combine those found in developed and developing countries, with an over-representation of infections (notably HIV and leprosy), and diabetes. The causes often result from social inequalities in health.</p><p><strong>Conclusion: </strong>This is the first comprehensive study of the diverse causes of neuropathy in a territory undergoing epidemiologic transition in the Amazon region.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"19 12","pages":"e0315760"},"PeriodicalIF":2.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embedding the patient voice into research on spontaneous preterm birth-themes from a Preterm Birth Advisory Council.","authors":"Gillian Corbett, Mandy Daly, Dylan Keegan, Patricia Horgan, Catriona Keyes, Larissa Luethe, Siobhan Corcoran, Fionnuala M McAuliffe","doi":"10.1371/journal.pone.0312370","DOIUrl":"https://doi.org/10.1371/journal.pone.0312370","url":null,"abstract":"<p><strong>Background: </strong>Spontaneous preterm birth (sPTB) has a deep immediate impact on patients but also alters their care and experience in subsequent pregnancies. There is an absence of the pregnant patient's voice in the research surrounding pregnancy at risk of sPTB.</p><p><strong>Materials/methods: </strong>The Preterm Birth Advisory Council was established at the National Maternity Hospital (NMH) in January 2023, to introduce and embed the patient voice in research into sPTB prevention. Council members include patients with lived experience of sPTB, patient advocate representatives and clinicians involved in sPTB preventative care. Topics around sPTB prevention were openly discussed with experts by experience and shared with sPTB advocacy groups. Responses were analysed for themes most important to those with lived experience. Ethical approval was granted by NMH Research Ethics Committee.</p><p><strong>Results: </strong>In total, seven experts by experience gave their views over the course of a three month period. Six key themes were observed: Clinical Outcomes in Preterm Birth Research. Low priority was placed on the modality, route and duration of interventions. The most important aspect of an intervention is its clinical efficacy. High tolerance thresholds were described in sacrifice for a positive pregnancy outcomePreterm Birth Preventative care as a patient-led care model. While medical and surgical interventions play their role in objectively reducing risk, much of pregnancy care after preterm birth centres on psychological support and continuity of care.Lack of awareness on risk factors for sPTB is an obstacle to timely referral and access to care. Patient experience of preterm birth prevention and timely interventions are improved where risk factor awareness exists.Importance of preconceptual counselling. The unexpected nature of adverse outcome adds additional trauma to the event itself. Anticipation of adverse event can improve sense of control, minimise trauma and increase ability to cope.The partner's experience of pregnancy at risk of preterm birth is largely missing from focus of research and clinical care. Peer-support, digital resources or partner handbooks may be helpful for support persons.Traumatic language included referring to a mid-trimester pregnancy loss or peri-viable preterm birth as a 'miscarriage.' Regarding language around preterm birth, there was no preferred term identified for women with risk factors or experience of preterm birth.</p><p><strong>Conclusions: </strong>The Preterm Birth Advisory Council places the voice of those impacted by preterm birth at the centre of research into its prevention. The themes identified may guide activities within this research area in local settings and international platforms. It is the council's hope that supported by their work, it will be the patient's voice that rings loudest in research in spontaneous preterm birth prevention.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"19 12","pages":"e0312370"},"PeriodicalIF":2.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food-based indices for the assessment of nutritive value and environmental impact of meals and diets: A systematic review protocol.","authors":"Eva-Leanne Thomas, David Livingstone, Anne P Nugent, Jayne V Woodside, Paul Brereton","doi":"10.1371/journal.pone.0315894","DOIUrl":"https://doi.org/10.1371/journal.pone.0315894","url":null,"abstract":"<p><p>Current food production and consumption practices are impacting both human and planetary health. Though these challenges are multifaceted, shifting to healthy dietary choices from sustainable food systems is one solution. Food-based labelling is a common public-health strategy aimed at influencing consumption practices, primarily displaying front-of-pack nutrition labelling to encourage healthier choices. Due to the dual impact of food on human and planetary health there is a growing need to additionally include environmental impact information. However, this potentially conflicting information could overwhelm consumers, necessitating a simplified approach that combines both nutritive and environmental values. Previous work has identified existing models, termed sustainable food profiling models, for labelling of individual food products based on their environmental impacts and nutritive value. Foods are rarely eaten in isolation and are often consumed as composite meals, which aggregate to diets. Therefore, it is important to identify indices that exist to assess or rank meals and diets according to their nutritional and environmental impacts. Following the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) 2015 guidelines, our systematic review protocol was registered with the International Prospective Register of Systematic Reviews (PROSPERO) on 01 May 2024 (PROSPERO registration ID = CRD42024537149). In the present protocol the methodology to identify and review existing food-based indices for the assessment of nutritive value and environmental impact of meals and diets and their intended public health purpose is described. Our primary research questions are: (i) what food-based indices assessing nutritive value and environmental impact exist for classification or ranking of meals or diets? and (ii) what are the methods used to create, and the key characteristics of, these indices?. The results are expected to increase understanding of, and highlight the variation in, the creation of combined measures for the assessment of nutritive value and environmental impact for meals and diets. Additionally, findings can be used to inform researchers, business, and policy actors on future approaches for labelling foods, meals, or diets in a way that supports healthy and sustainable meal choices and diets.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"19 12","pages":"e0315894"},"PeriodicalIF":2.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population travel increases the risk of Plasmodium falciparum infection in the highland population of Gardula Zone, South Ethiopia: A longitudinal study.","authors":"Muluken Assefa, Fekadu Massebo, Temesgen Ashine, Teklu Wegayehu","doi":"10.1371/journal.pone.0315900","DOIUrl":"https://doi.org/10.1371/journal.pone.0315900","url":null,"abstract":"<p><p>Population movement influences malaria epidemiology and can be a threat to malaria control and elimination. In Ethiopia, highland dwellers often travel to lowland areas where malaria is endemic. The current study aimed to assess the incidence of malaria and risk factors among dwellers in two highland villages of the former Dirashe District (now Gardula Zone), South Ethiopia. A longitudinal study was conducted from 10/05/2018 to 30/11/2018. A total of 1672 individuals from 329 households were recruited via a systematic random sampling technique. Blood samples were collected from all consented family members. The study participants were interviewed via a pretested questionnaire. Bivariate and multivariate analyses were conducted to determine risk factors associated with malaria infection. A total of 4,884 blood samples were screened for malaria parasites in three rounds of surveys. Among those, 82 slides were positive for malaria parasites, 70 (85%) of which were collected during active case detection, and the remaining 12 (15%) were captured by passive case detection. Plasmodium falciparum accounted for 69.5% (57), and the remaining 30.5% (25) were Plasmodium vivax. The incidence of malaria in the highlands of Dirashe District was 0.2 infections per person-year at risk. Inhabitants who traveled to lowlands in the past 30 days (AOR = 2.60, 95% CI: 1.27-5.33) had a significantly greater risk of contracting Plasmodium falciparum infection. Those people traveling in May and November, those participants who had no formal education and agricultural workers, had a greater risk of developing malaria infection. Low bed net ownership (63.2%) and use (52.9%) have been documented among highland populations. This study revealed that people who travel from highlands to malaria-endemic lowland areas for agriculture are at increased risk of developing malaria. Hence, malaria interventions targeting travelers should be implemented to reduce the imported malaria burden in highlands.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"19 12","pages":"e0315900"},"PeriodicalIF":2.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}