PLoS ONE最新文献

{"title":"Expression of Concern: Electromyographic activity of pelvic floor muscles in different positions during the use an innovative vaginal educator: Cross-sectional study.","authors":"","doi":"10.1371/journal.pone.0360775","DOIUrl":"https://doi.org/10.1371/journal.pone.0360775","url":null,"abstract":"","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"21 10","pages":"e0360775"},"PeriodicalIF":2.8,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Concern: Spatial pattern of China's rural digital economy based on subjective-Objective evaluation: Evidence from 2085 counties.","authors":"","doi":"10.1371/journal.pone.0360864","DOIUrl":"https://doi.org/10.1371/journal.pone.0360864","url":null,"abstract":"","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"21 10","pages":"e0360864"},"PeriodicalIF":2.8,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Concern: Decision analysis of PPP project's parties based on deep consumer participation.","authors":"","doi":"10.1371/journal.pone.0360779","DOIUrl":"https://doi.org/10.1371/journal.pone.0360779","url":null,"abstract":"","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"21 10","pages":"e0360779"},"PeriodicalIF":2.8,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction: Pyrroloquinoline Quinine Inhibits RANKL-Mediated Expression of NFATc1 in Part via Suppression of c-Fos in Mouse Bone Marrow Cells and Inhibits Wear Particle-Induced Osteolysis in Mice.","authors":"","doi":"10.1371/journal.pone.0360868","DOIUrl":"https://doi.org/10.1371/journal.pone.0360868","url":null,"abstract":"","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"21 10","pages":"e0360868"},"PeriodicalIF":2.8,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Concern: Evaluation of China's provincial digital economy development level and its coupling coordination relationship.","authors":"","doi":"10.1371/journal.pone.0360865","DOIUrl":"https://doi.org/10.1371/journal.pone.0360865","url":null,"abstract":"","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"21 10","pages":"e0360865"},"PeriodicalIF":2.8,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Reduced SOD2 expression does not influence prion disease course or pathology in mice.","authors":"Simote T Foliaki, Brent Race, Katie Williams, Chase Baune, Bradley R Groveman, Cathryn L Haigh","doi":"10.1371/journal.pone.0360777","DOIUrl":"https://doi.org/10.1371/journal.pone.0360777","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1371/journal.pone.0259597.].</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"21 10","pages":"e0360777"},"PeriodicalIF":2.8,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of SGK1 as a candidate regulator associated with myeloid cell senescence in ME/CFS: An integrative transcriptomic and experimental study.","authors":"Yan Jin, Rui Qin, Wan Li, Jin Liu, Xinghua Chen","doi":"10.1371/journal.pone.0359082","DOIUrl":"10.1371/journal.pone.0359082","url":null,"abstract":"<p><strong>Background: </strong>Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex illness lacking objective diagnostic biomarkers and clear pathophysiological understanding. Patients exhibit immune dysregulation and chronic inflammation reminiscent of aging phenotypes, yet the role of myeloid cell senescence in this disease remains unexplored.</p><p><strong>Methods: </strong>A stepwise integrative transcriptomic and computational strategy was employed. Differentially expressed genes were identified from the bulk transcriptomic dataset GSE14577. Weighted gene co-expression network analysis was performed to identify key modules correlated with the ME/CFS phenotype. Candidate genes were obtained by intersecting these modules with aging-related genes from the GenAge database. Core hub genes were further selected using LASSO regression and random forest machine learning algorithms. Cell type-specific expression was mapped using single-cell RNA sequencing data GSE214283, and functional validation was conducted via siRNA knockdown in a THP-1 cell senescence model.</p><p><strong>Results: </strong>Integrative analysis identified HMGCR and SGK1 as core hub genes. Functional enrichment analysis revealed that candidate genes were enriched in pathways related to cellular senescence and inflammation. Single-cell transcriptomics analysis suggested that SGK1 expression was enriched in myeloid cell populations, including monocytes and neutrophils, in individuals with ME/CFS, whereas HMGCR showed a broader expression pattern. Single-gene GSEA indicated that SGK1 was associated with autophagy and lysosomal function. In vitro experiments showed that SGK1 expression was significantly upregulated in D-galactose-induced senescent THP-1 cells, and SGK1 knockdown markedly reduced the mRNA expression levels of senescence markers p16, p21, and p53.</p><p><strong>Conclusion: </strong>This study identifies SGK1 as a candidate molecule associated with myeloid cell senescence-related alterations in ME/CFS. Integrated transcriptomic, single-cell, and experimental analyses suggest a potential link between SGK1 expression and aging-associated immune alterations. However, further validation in primary ME/CFS-derived immune cells and clinical cohorts is required to determine the biological significance and clinical relevance of SGK1.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"21 10","pages":"e0359082"},"PeriodicalIF":2.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13674702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149207491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Economic valuation of ecosystem services in agriculture and agroforestry: A meta-analysis.","authors":"Mahugnon Maxime Obe, Cokou Patrice Kpadé, Alphonse Singbo, Terence Epule Epule, Vincent Poirier","doi":"10.1371/journal.pone.0360792","DOIUrl":"10.1371/journal.pone.0360792","url":null,"abstract":"<p><p>The economic valuation of ecosystem services in agriculture and agroforestry is crucial for sustainable development. However, valuation methods vary significantly across contexts. Using the PRISMA framework, we systematically reviewed 146 studies, combined effect sizes through meta-analytic modeling, and conducted spatial autocorrelation analyses to explore geographic patterns. Market price valuation (MPV) and contingent valuation (CVM) are the most used methods in ecosystem service assessments, accounting for 25.8% and 20.8% of studies, respectively. The CVM and life cycle impact assessment (LCIA) approaches play a key role in interdisciplinary research, connecting all ecosystem service categories. Analysis revealed extreme heterogeneity and significant publication bias for cultural (CULT), provisioning (PROV), and regulating (REG) services. Valuation outcomes are strongly influenced by the choice of methods (such as MPV and benefit transfer methods) and unit standardization practices, with effect sizes decreasing when values are standardized to United States dollars per ha per year (USD/ha/year). Spatial autocorrelation was minimal, indicating the importance of local context in the valuation results. Temporal trends indicate episodic changes in the relative effectiveness of valuation approaches. Empirically, MPV showed greater robustness (18%) than CVM, which exhibited greater sensitivity to data constraints (16%). These findings highlight the need to prioritize data quality and context specificity within standardized valuation frameworks to improve policy relevance and comparability in ecosystem service assessments, particularly for underrepresented CULT and supporting (SUP) services.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"21 10","pages":"e0360792"},"PeriodicalIF":2.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13674745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149207792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rates of nonproliferative diabetic retinopathy (NPDR) regression among patients with very mild or mild NPDR in an Indian Health Service teleophthalmology program, 2015 - 2020.","authors":"Sven-Erik Bursell, Stephanie J Fonda, Kaitlynn Borik, Jeff Stainback, Dara Shahon","doi":"10.1371/journal.pone.0361052","DOIUrl":"10.1371/journal.pone.0361052","url":null,"abstract":"<p><p>Diabetic retinopathy (DR) regression is not regularly reported; yet understanding of its prevalence in early-stage DR may impact strategies for eye care management. This study's primary aim was to examine DR regression in patients initially diagnosed with very mild or mild nonproliferative DR (NPDR). A secondary aim was to assess the association between retinopathy regression and diabetes therapy, diabetes duration, and Hemoglobin A1c (HbA1c). This was a retrospective, longitudinal cohort study using medical record data from the United States's Indian Health Service's teleophthalmology program for DR evaluation. Baseline exams occurred in 2015-2017 and follow-up (FU) exams occurred within 3 years of baseline. There were 2,348 patients included in this cohort with very mild NPDR at baseline and 705 patients with mild NPDR. For the whole sample, mean age was 56 ± 12.8 years, 55% were female, diabetes duration was 13.0 ± 9.1 years, and HbA1c was 8.8 ± 2.1%. Proportions for DR regression were 0.47 (CI = .45 - .50) (from very mild NPDR) and 0.36 (CI = 0.33-0.40) (from mild NPDR). Proportions for any DR progression were 0.27 (CI = 0.25-0.29) (from very mild NPDR) and 0.34 (CI = 0.30-0.38) (from mild NPDR). Multivariable analyses showed that lower HbA1c levels were associated with greater odds of diabetic retinopathy regression in patients with either very mild or mild baseline NPDR, whereas insulin use was associated with lower odds of regression. Shorter diabetes duration was associated with greater odds of regression only among patients with very mild NPDR at baseline. DR regression was more frequent than DR progression particularly for patients initially with very mild NPDR. DR reversal was more likely in early-stage DR. Behavioral modifications such as increased physical activity and weight loss that lower HbA1c and subsequent risks for DR progression are important to emphasize in diabetic eye care.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"21 10","pages":"e0361052"},"PeriodicalIF":2.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13674746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149207875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between the use of fillers and Japanese proficiency: Focusing on OPI level assessment.","authors":"Zuohao Chen","doi":"10.1371/journal.pone.0360747","DOIUrl":"10.1371/journal.pone.0360747","url":null,"abstract":"<p><p>This study employed a Cumulative Link Mixed Model (CLMM) to examine the relationship between Japanese learners' filler use and OPI (Oral Proficiency Interview) level ratings. The results showed no significant association with proficiency for the i(い)-group, u(う)-group, ano(あの)-group, and eeto(ええと)-group fillers. In contrast, the a(あ)-group, e(え)-group, o(お)-group, and n(ん)-group fillers were significantly negatively associated with higher proficiency, while the use of maa(まあ)-group and sono(その)-group fillers was significantly positively associated with higher proficiency. Furthermore, an analysis of maa(まあ)-group and sono(その)-group fillers based on their functions and discourse roles showed that turn-internal maa(まあ) and inferential/re-presenting sono(その) require at least two clauses to be produced. This indicates a connection with paragraph-level language production, which is one of the criteria for 'Text Type' in OPI assessment. It was also found that the use of these fillers with such functions is significantly positively associated with higher proficiency. On the other hand, for fillers such as ano(あの) and eeto(ええと), as well as maa(まあ) used in adjacency pairs, no significant increase with proficiency was observed, confirming that their use is not associated with paragraph-level production. The use of fillers linked to paragraph-level production can be considered one of the key linguistic factors that can help explain the relationship between filler use and the development of oral language proficiency.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"21 10","pages":"e0360747"},"PeriodicalIF":2.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13674766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149207887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}