PLoS ONE最新文献

{"title":"Examining the relationship between income and both mental and physical health among adults in the UK: Analysis of 12 waves (2009-2022) of Understanding Society.","authors":"Howard Robert Reed, Daniel Nettle, Fiorella Parra-Mujica, Graham Stark, Richard Wilkinson, Matthew Thomas Johnson, Elliott Aidan Johnson","doi":"10.1371/journal.pone.0316792","DOIUrl":"10.1371/journal.pone.0316792","url":null,"abstract":"<p><p>There is growing evidence of a causal relationship between income and health. At the same time, pressure on reactive health and care services in the UK is increasing. Previous work to quantify the relationship has focused on particular age groups, conditions, or single-item self-rated health. This article reports findings from a study that aimed to provide more comprehensive estimates with an objective of creating an evidential basis for microsimulation modelling of upstream income interventions. We analyse the relationship between income and two health measures - SF-12 Mental Component Summary (MCS-12) and Physical Component Summary (PCS-12) - across 12 waves (2009/11-2020/22) of Understanding Society: The UK Household Longitudinal Study. Using a 'within-between' model (Model 1), we find that increases in income compared with an individual's average and a higher income compared with the sample average is associated with better mental health (higher MCS-12 score) and better functional physical health (higher PCS-12 score). However, for a given increase in household income (say £100 per month), the association with better mental and physical health is smaller at higher incomes. This suggests that redistribution from high-income to lower income households would increase average population physical and mental health, other things being equal. Using a random-effects logistic regression (Model 2), we similarly find that average income quintile is inversely and monotonically associated with the probability of having clinically significant symptoms of depressive disorders (MCS-12 ≤ 45.6) and physical health problems (PCS-12 score ≤ 50.0), with smaller changes in these probabilities from increases in income at higher points in the income distribution. These findings facilitate microsimulation modelling including an estimation of the impact of changes in QALYs, from changes in income, enabling a more detailed and complete understanding of which socioeconomic interventions might begin to address some of the causes of long-term health conditions that are underpinned by socioeconomic determinants.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0316792"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medical students' and educators' opinions of teleconsultation in practice and undergraduate education: A UK-based mixed-methods study.","authors":"Lisa-Christin Wetzlmair-Kephart, Andrew O'Malley, Veronica O'Carroll","doi":"10.1371/journal.pone.0302088","DOIUrl":"10.1371/journal.pone.0302088","url":null,"abstract":"<p><strong>Introduction: </strong>As information and communication technology continues to shape the healthcare landscape, future medical practitioners need to be equipped with skills and competencies that ensure safe, high-quality, and person-centred healthcare in a digitised healthcare system. This study investigated undergraduate medical students' and medical educators' opinions of teleconsultation practice in general and their opinions of teleconsultation education.</p><p><strong>Methods: </strong>This study used a cross-sectional, mixed-methods approach, utilising the additional coverage design to sequence and integrate qualitative and quantitative data. An online questionnaire was sent out to all medical schools in the UK, inviting undergraduate medical students and medical educators to participate. Questionnaire participants were given the opportunity to take part in a qualitative semi-structured interview. Descriptive and correlation analyses and a thematic analysis were conducted.</p><p><strong>Results: </strong>A total of 248 participants completed the questionnaire and 23 interviews were conducted. Saving time and the reduced risks of transmitting infectious diseases were identified as common advantages of using teleconsultation. However, concerns about confidentiality and accessibility to services were expressed by students and educators. Eight themes were identified from the thematic analysis. The themes relevant to teleconsultation practice were (1) The benefit of teleconsultations, (2) A second-best option, (3) Patient choice, (4) Teleconsultations differ from in-person interactions, and (5) Impact on the healthcare system. The themes relevant to teleconsultation education were (6) Considerations and reflections on required skills, (7) Learning and teaching content, and (8) The future of teleconsultation education.</p><p><strong>Discussion: </strong>The results of this study have implications for both medical practice and education. Patient confidentiality, safety, respecting patients' preferences, and accessibility are important considerations for implementing teleconsultations in practice. Education should focus on assessing the appropriateness of teleconsultations, offering accessible and equal care, and developing skills for effective communication and clinical reasoning. High-quality teleconsultation education can influence teleconsultation practice.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0302088"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OM-VST: A video action recognition model based on optimized downsampling module combined with multi-scale feature fusion.","authors":"Xiaozhong Geng, Cheng Chen, Ping Yu, Baijin Liu, Weixin Hu, Qipeng Liang, Xintong Zhang","doi":"10.1371/journal.pone.0318884","DOIUrl":"10.1371/journal.pone.0318884","url":null,"abstract":"<p><p>Video classification, as an essential task in computer vision, aims to identify and label video content using computer technology automatically. However, the current mainstream video classification models face two significant challenges in practical applications: first, the classification accuracy is not high, which is mainly attributed to the complexity and diversity of video data, including factors such as subtle differences between different categories, background interference, and illumination variations; and second, the number of model training parameters is too high resulting in longer training time and increased energy consumption. To solve these problems, we propose the OM-Video Swin Transformer (OM-VST) model. This model adds a multi-scale feature fusion module with an optimized downsampling module based on a Video Swin Transformer (VST) to improve the model's ability to perceive and characterize feature information. To verify the performance of the OM-VST model, we conducted comparison experiments between it and mainstream video classification models, such as VST, SlowFast, and TSM, on a public dataset. The results show that the accuracy of the OM-VST model is improved by 2.81% while the number of parameters is reduced by 54.7%. This improvement significantly enhances the model's accuracy in video classification tasks and effectively reduces the number of parameters during model training.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0318884"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary investigation of anti-fatigue effects and potential mechanisms of meiju oral liquid in mouse and zebrafish models.","authors":"Lanlan Zhang, Jingcheng Zhao, Xi Zhou, Maitinuer Maiwulanjiang","doi":"10.1371/journal.pone.0316761","DOIUrl":"10.1371/journal.pone.0316761","url":null,"abstract":"<p><p>Meiju Oral Liquid (MOL), a representative medicinal formula in China, stems from the traditional use of specific Chinese medicinal herbs known for their anti-fatigue properties, including rose, jujube, chicory, and wolfberry. While these individual herbs have been recognized for their benefits, the formulation of MOL itself has not been extensively studied. This study was designed to evaluate the potential anti-fatigue effects of MOL, prepared from these natural herbs, and to explore its underlying mechanisms. In this research, both mouse and zebrafish models were utilized to investigate the anti-fatigue effects of MOL. Chemical characterization of MOL and identification of bioactive compounds in serum were conducted using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). The results demonstrated that MOL significantly prolonged the weight-bearing swimming time in mice, increased hepatic and muscle glycogen content, and reduced serum levels of blood urea nitrogen, blood lactate, and inflammatory markers (IL-1β, IL-6, TNF-α, and NO). Furthermore, MOL down-regulated the expression of NOX4 and TNF-α proteins while up-regulating p-PI3K and p-AKT proteins in the liver tissues of fatigued mice. In zebrafish models, MOL exhibited protective effects against sodium sulfite-induced lethality, enhanced high-speed motion trajectories, and increased movement distances in both normal and fatigued zebrafish. Additionally, MOL downregulated IL-1β, IL-6, TNF-α, and TNF-β mRNA levels while up-regulating PI3K and AKT1 mRNA levels in fatigued zebrafish. These findings suggested that the anti-fatigue effects of MOL may be mediated through the activation of the PI3K/AKT signaling pathway as well as the inhibition of TNF-α and NOX4 expression. In addition, a total of ninety-four chemical components were identified in MOL, with twenty-three migration compounds detected in mouse serum. These migration compounds are likely the primary active agents, contributing to the reduction of metabolite accumulation, enhancement of glycogen synthesis, and suppression of inflammatory responses. Taken together, our findings underscore the potential anti-fatigue effects of MOL, warranting further investigation into its therapeutic applications and the specific roles of its bioactive compounds.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0316761"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An exploration of the support received by mothers for kangaroo mother care practice along the health facility-community continuum in a sub-district of Northern Karnataka, India.","authors":"Maryann Washington, Leah Macaden, Prem K Mony, Sumithra Selvam, Annetta Smith","doi":"10.1371/journal.pone.0308738","DOIUrl":"10.1371/journal.pone.0308738","url":null,"abstract":"<p><strong>Introduction: </strong>Early initiation with optimal duration of Kangaroo Mother Care (KMC), for all stable small babies (<2000grams at birth), is essential for accelerated reduction of neonatal mortality. The purpose of this paper is to explore the support received by mothers along the health facility-community continuum and its association with KMC practice.</p><p><strong>Methods: </strong>All live small babies aged >  4 weeks of life, who were residing in the Gangawati sub-district, were recruited on a rolling basis (Dec 2017-Sept 2018) to obtain the estimated sample size of 210. Mother-baby dyads were visited in their homes to collect information [knowledge, attitude, and support received] for KMC initiation and maintenance till required. Secondary data on KMC duration was obtained from the district-wide project database.</p><p><strong>Results: </strong>A total of 209 mothers with 227 small babies were interviewed (18 had twins). The mothers had a mean age of 23 (±4) years; and 7(±5) years of education, with 5 (±2) family members >  18 years in their households. More than half (51%) of the babies were female with a mean age of 35.6 (±7.5)days/ 4-6weeks and mean birth weight of 1693.6 (±221.4)grams irrespective of gestational age; 21.6% of whom were ≤ 1500g at birth. Most of the babies 205 (90.3%) were initiated on KMC at the health facility. The score obtained for KMC initiation [45%} and KMC maintenance support at the health facility [51.3%] was minimal. Multiple regression linear analysis showed that overall KMC support at the health facility was significantly higher for first-time mothers [β coefficient -1.54 (95% CI -2.87, -0.22)] and better knowledge scores on KMC [β coefficient 0.21 (05% CI 0.01, 0.42)]. KMC maintenance support was significantly higher for first-time mothers [β coeff -3.62 (95% CI -6.29, -0.96)] and for mothers whose babies had lower birth weights [β coeff -4.27 (95% CI -7.50, -1.05)].</p><p><strong>Conclusion: </strong>Mothers require support to initiate and continue KMC along the health facility-community continuum (S1 Table). The role of support at home would require further exploration to determine its association with KMC practice.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0308738"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human capital and regional disparities: Advancing accounting frameworks with education, health, and population dynamics.","authors":"Shuning Chen, Xiangdan Piao, Jun Xie, Shunsuke Managi","doi":"10.1371/journal.pone.0315166","DOIUrl":"10.1371/journal.pone.0315166","url":null,"abstract":"<p><p>This study advances the inclusive wealth accounting of human capital (HC) to improve global research on the valuation of HC for sustainability. By innovatively integrating complex population dynamics, including schooling and labor force participation, and using a net present value (NPV) valuation method aligned with capital budgeting principles, we quantitatively measure HC in 165 countries. As a methodological advancement, we use a unified framework that incorporates education, health and economic participation via the measurement of life expectancy in different life stages to inform sustainable development investments. Our analysis from 1990 to 2020 reveals significant differences in HC development across countries. While education is strongly correlated with GDP growth, disparities in health and economic participation are critical barriers to long-term HC accumulation. Our findings argue for a comprehensive policy approach that goes beyond investing in education for its financial benefits and includes substantial improvements in health and economic opportunities to promote more equitable HC growth. We emphasize the need to incorporate complex population dynamics into HC assessments to better understand and strengthen the interdependencies between these critical factors, with the aim of reducing global development gaps.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0315166"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of drug target interaction based on under sampling strategy and random forest algorithm.","authors":"Feng Chen, Zhigang Zhao, Zheng Ren, Kun Lu, Yang Yu, Wenyan Wang","doi":"10.1371/journal.pone.0318420","DOIUrl":"10.1371/journal.pone.0318420","url":null,"abstract":"<p><p>Drug target interactions (DTIs) play a crucial role in drug discovery and development. The prediction of DTIs based on computational method can effectively assist the experimental techniques for DTIs identification, which are time-consuming and expensive. However, the current computational models suffer from low accuracy and high false positive rate in the prediction of DTIs, especially for datasets with extremely unbalanced sample categories. To accurately identify the interaction between drugs and target proteins, a variety of descriptors that fully show the characteristic information of drugs and targets are extracted and applied to the integrated method random forest (RF) in this work. Here, the random projection method is adopted to reduce the feature dimension such that simplify the model calculation. In addition, to balance the number of samples in different categories, a down sampling method NearMiss (NM) which can control the number of samples is used. Based on the gold standard datasets (nuclear receptors, ion channel, GPCRs and enzymes), the proposed method achieves the auROC of 92.26%, 98.21%, 97.65%, 99.33%, respectively. The experimental results show that the proposed method yields significantly higher performance than that of state-of-the-art methods in predicting drug target interaction.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0318420"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Happy-Productive worker thesis: The role of work characteristics, gender, and age.","authors":"Jaime A Bayona, William F Durán, Jesús Perdomo-Ortiz, Delio I Castañeda, Carlos A Valencia, Pauline Fatien Diochon, Diego F Alvarado","doi":"10.1371/journal.pone.0316656","DOIUrl":"10.1371/journal.pone.0316656","url":null,"abstract":"<p><p>Happy-productive worker thesis (HPWT) research predicts four configurations depending on well-being and performance levels, one synergistic and three antagonists; however, there has been some discrepancy in the expected results of HPWT, as there are some inclusive results about the specific characteristics that lead to each one of the predicted groups. In this study, we face these discrepancies using a three-configuration model that is more realistic in the organizational context, and exploring how work characteristics, gender, and age can predict workers' membership in such configurations. We performed multinomial logistic regressions using a sample of 504 Colombian workers and their supervisors from different economic sectors. The results indicated that different work characteristics are associated with the membership of workers in each group, and how this membership varies depending on gender and age group. Our findings offer new research and practice insights about the role of HPWT in HRM (human resources management).</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0316656"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promoting language and literacy through shared book reading in the NICU: A scoping review.","authors":"Lama K Farran, Sharon L Leslie, Susan N Brasher","doi":"10.1371/journal.pone.0318690","DOIUrl":"10.1371/journal.pone.0318690","url":null,"abstract":"<p><strong>Background: </strong>Infants in the neonatal intensive care unit (NICU) are at a heightened risk for language and literacy delays and disorders. Despite the well-established empirical support for early shared reading, the available evidence to date has been scant, revealing mixed results. This study sought to characterize current research on shared reading in the NICU using a scoping review methodology.</p><p><strong>Methods: </strong>Studies were eligible for inclusion if they were peer-reviewed, written in the English language, focused on human infants in the NICU, and published between January 1, 2003, and December 31, 2023. No population age range was applied, and quantitative, qualitative, or mixed methods designs were considered. Database searches yielded 338 articles with only eight articles meeting eligibility criteria for inclusion.</p><p><strong>Conclusion: </strong>In spite of a modest number of studies on this topic, utilizing limited methodologies, the evidence from this scoping review shows the benefits of shared reading for infants and their caregivers during their NICU stay. Expanding such efforts by embedding shared reading as part of standard practice is recommended.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0318690"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the silent threat: Investigating asymptomatic plasmodium infections in Gorgora, Ethiopia through microscopy and loop-mediated isothermal amplification.","authors":"Tena Cherkos, Adane Derso, Banchamlak Tegegne, Abebe Birhanu, Kassahun Cherkos, Zufan Yiheyis Abreham, Banchayehu Getnet, Tsedenya Gebeyehu, Mulat Yimer, Tegegne Eshetu, Wossenseged Lemma, Aberham Abere, Yalewayker Tegegne, Dylan R Pillai","doi":"10.1371/journal.pone.0313746","DOIUrl":"10.1371/journal.pone.0313746","url":null,"abstract":"<p><strong>Background: </strong>The asymptomatic carriers of the Plasmodium parasite represent a large hidden reservoir of Plasmodium species. These individuals can carry microscopically detectable levels and sub-microscopic levels of Plasmodium parasitemia. As a result, the use of clinical diagnostic methods, such as Rapid Diagnosis Tests (RDTs) and Giemsa microscopy leads to underestimation of the burden of asymptomatic malaria. Thus, the use of highly sensitive molecular methods such as loop-mediated isothermal amplification techniques (LAMP) helps to overcome these limitations and is crucial to reporting the true burden of the disease.</p><p><strong>Objective: </strong>This study aimed to determine the prevalence of Asymptomatic Plasmodium infections (APIs), and evaluate light microscopy for the detection of APIs using the LAMP method as a reference in Gorgora, Western Dembia district, Northwest Ethiopia.</p><p><strong>Method: </strong>A community-based cross-sectional survey was carried out from May 17 to June 30, 2023, among households located in particular kebeles at Gorgora. The study participants were chosen using a multi-stage sampling technique. One Hundred Fifteen households and one household member from each household were selected using systematic random and simple random sampling techniques, respectively. To gather sociodemographic data, semi-structured questionnaires were used. Capillary blood samples were obtained from each study participant and screened for Plasmodium species infections using a LAMP kit and light microscopy. The data were entered into Epi Data version 4.6 and exported to SPSS version 25 for analysis.</p><p><strong>Results: </strong>The prevalence of APIs through light microscopy examination and LAMP analysis was 6.1% (95%CI: 2.5-12.1) and 11.3% (95%CI: 6.2-18.6) respectively. Using LAMP as a reference, the sensitivity and specificity of microscopy were 53.8% and 100% respectively. Microscopy examination missed six Plasmodium infections that were positive by LAMP analysis. A measure of agreement between LAMP and Microscopy was found to be 0.67(k = 0.67).</p><p><strong>Conclusions: </strong>A significant proportion of APIs was found which likely act as a reservoir of transmission. This study also showed a significant number of APIs were missed by microscopy techniques. Thus, efforts to control and eliminate malaria should also consider these sub-microscopic infections.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0313746"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}