PLoS ONE最新文献

{"title":"Assessing anthropogenic impact on the habitat of threatened rock cavy (Kerodon rupestris) through its alarm calls.","authors":"Wesley N Almeida, Kamila S Barros, Sérgio L G Nogueira-Filho, Selene S C Nogueira","doi":"10.1371/journal.pone.0323711","DOIUrl":"https://doi.org/10.1371/journal.pone.0323711","url":null,"abstract":"<p><p>Acoustic monitoring is emerging as a key tool in wildlife conservation, especially for species in inaccessible habitats like the rock cavy (Kerodon rupestris), an endangered species native to Brazil's threatened Caatinga biome. Emotional stress from threatening situations affects breathing, heart rate, and vocal muscle tension, altering vocal acoustic parameters. This allows researchers to gauge the animal's environmental perception through its vocalizations. We aimed to evaluate emotional disturbance indicators in free-range rock cavies' vocalizations to suggest an acoustic index during threats. We compared calls from rock cavies in two areas with similar habitats but that differ in terms of anthropic impacts. Area 1 (A1) is near urban areas and disturbed by livestock and dogs, and Area 2 (A2) is farther from urban areas and free from human disturbance. Data on calls and behaviors were collected ad libitum in both areas. The alarm whistle call, making up 73.5% of total calls, was most common. Across 108 observation hours per area, 392 alarm whistle calls were recorded, with more calls in A1 than A2 (223 vs. 169; Chi-square = 29.44, DF = 1, P < 0.001). This resulted in a 32% higher hourly call rate in A1 (2.6 calls/h vs. 1.6 calls/h). Both male and female cavies in A1 had higher high-frequency (F1, 388 = 7.80, P = 0.005) and peak-frequency calls (F1, 388 = 21.32, P < 0.001). Given the similar landscape and resource availability in both areas, the differences in call emission rate and parameters are likely linked to emotional responses to human disturbances in A1. Thus, alarm whistle calls at an hourly rate of 2.6 calls/h or higher, with high-frequency and peak-frequency at or above 7222 Hz and 2603 Hz, can indicate anthropogenic disturbance in the Caatinga biome, aiding remote monitoring efforts.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0323711"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the impacts of inter-basin water transfer projects on ecosystem services in water source areas: Evidence from the Hanjiang River Basin.","authors":"Nana Zhuang, Min Wang, Chenyi Shi, Shen Fu, Qiyuan Yang, Conghui Ding, Yiao Ouyang, Hai Liu","doi":"10.1371/journal.pone.0323068","DOIUrl":"https://doi.org/10.1371/journal.pone.0323068","url":null,"abstract":"<p><p>Inter-basin water transfer projects (IBWT) are a key strategy for alleviating regional water shortages. However, studies on the long-term effects of such projects on ecosystem services (ESs) in water source areas, as well as their spatiotemporal evolution, remain insufficient. In particular, the specific impacts of the entire project lifecycle (project initiation, dam heightening, project operation, and ecological restoration) on ESs need further exploration. This study focuses on the Middle Route of the South-to-North Water Diversion Project in China, utilizing multi-source datasets (land use and land cover, meteorological data, soil texture, digital elevation models, normalized difference vegetation index, and net primary productivity), applying the biophysical model method to examine the spatiotemporal variations in ESs across the Hanjiang River Basin over the past three decades, and investigates the impact of IBWT on the ESs of water source areas. The findings reveal: (1) During the project initiation phase (2000-2010), vegetation restoration strategies enhanced soil retention by over 60%, demonstrating that simultaneous project-ecological implementation can mitigate habitat degradation risks. (2) The dam heightening phase (2005-2010) drove spatial reconfiguration of water-energy fluxes, leading to declines in water conservation and carbon sequestration in the midstream region, while enhancing flood mitigation and climate regulation in the reservoir area, unveiling the reshaping mechanisms of dam heightening on ESs supply patterns. (3) The operation phase exhibited significant temporal heterogeneity: initial operation (2010-2015) saw a sharp decline in water conservation (>40%) and soil retention (>60%) due to hydrological disturbances, whereas sustained operation (2015-2020) restored water conservation by nearly 70% and soil retention by over 40% through ecological restoration, alongside a net increase of 14.14% in carbon sequestration, confirming the time-lag compensation effects of restoration measures and the dynamic interplay between ecological restoration and project interventions. This research presents empirical evidence supporting the sustainable management and ecological restoration of IBWT, emphasizing the need to balance spatial water allocation with ecological conservation.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0323068"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the ORF: Paralog-specific regulation of RPS7/eS7 mRNAs via 3'-UTRs and promoter sequences.","authors":"Sachiko Hayashi, Tohru Yoshihisa","doi":"10.1371/journal.pone.0324525","DOIUrl":"https://doi.org/10.1371/journal.pone.0324525","url":null,"abstract":"<p><p>In a classical view, each paralogous ribosomal protein (RP) is equally synthesized and integrated into the ribosome. Therefore, RP-paralog mRNAs are generally believed to be similarly regulated on their transcription and/or stability. In this paper, we report that two Rps7p/eS7 paralogs of Saccharomyces cerevisiae are differently regulated; deletion of RPS7A upregulates RPS7B paralogous mRNA expression but not vice versa. Their 3'-UTR sequences critically regulated the stabilities of both RPS7A and RPS7B mRNAs. Alterations in these sequences led to a diminished expression of RPS7A and RPS7B mRNAs in a transcript-dependent manner, suggesting that RPS7-paralog mRNAs have different properties for their expression and/or stability. The C-terminal tagging of the ORF and mutation analyses in the 3'-UTR indicate that both RPS7-paralog mRNAs critically rely on their 3'-UTRs for mRNA expressions and/or stabilities. We also found that activities of both RPS7A and RPS7B promoters are regulated by abundance of Rps7Ap and that Fhl1p, a key transcriptional regulator of RP genes, is essential for transcription of RPS7B but not RPS7A while simultaneous deletion of a consensus sequence for Fhl1p in the RPS7A promoter region and the FHL1 gene completely abolishes the promoter activity. These results indicate that yeast has a distinct buffering system for Rps7p production between the two RPS7-paralogs, which is sensitive to variation on their 3'-UTRs and is partially mediated in a transcription-dependent manner.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0324525"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"T7 RNA polymerase-based gene expression from a transcriptionally silent rDNA spacer in the endosymbiont-harboring trypanosomatid Angomonas deanei.","authors":"Lena Kröninger, Anay K Maurya, Christian Stiebeling, Florian P Stirba, Zio Kim, Eva C M Nowack","doi":"10.1371/journal.pone.0322611","DOIUrl":"https://doi.org/10.1371/journal.pone.0322611","url":null,"abstract":"<p><p>Eukaryotic life has been shaped fundamentally by the integration of bacterial endosymbionts. The trypanosomatid Angomonas deanei that contains a β-proteobacterial endosymbiont, represents an emerging model to elucidate initial steps in symbiont integration. Although the repertoire of genetic tools for A. deanei is growing, no conditional gene expression system is available yet, which would be key for the functional characterization of essential or expression of toxic proteins. Development of a conditional expression system based on endogenous RNA polymerase II (POLII) is hampered by the absence of information on transcription signals in A. deanei as well as the unusual genetic system used in the Trypanosomatidae that relies on read-through transcription. This mode of transcription can result in polar effects when manipulating expression of genes in their endogenous loci. Finally, only a few resistance markers are available for A. deanei yet, restricting the number of genetic modifications that can be introduced into one strain. To increase the range of possible genetic manipulations in A. deanei, and in particular, build the base for a conditional expression system that does not interfere with the endogenous gene expression machinery, here we (i) implemented two new drug resistance markers, (ii) identified the spacer upstream of the rDNA array on chromosome 13 as transcriptionally silent genomic locus, and (iii) used this locus for engineering an ectopic expression system that depends on the T7 RNA polymerase expressed from the δ-amastin locus. We show that transgene expression in this system is independent of the activity of endogenous RNA polymerases, reaches expression levels similar to the previously described POLII-dependent expression from the γ-amastin locus, and can be applied for studying endosymbiosis. In sum, the new tools expand the possibilities for genetic manipulations of A. deanei and provide a solid base for the development of an ectopic conditional expression system.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0322611"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence on the Porter hypothesis: China's Resource Tax Law may be a path to achieve corporate sustainable development.","authors":"Xiaoqi Zhang, Yu He","doi":"10.1371/journal.pone.0323668","DOIUrl":"https://doi.org/10.1371/journal.pone.0323668","url":null,"abstract":"<p><p>Promoting utilization efficiency and sustainable energy development is crucial for achieving sustainable social development in China, the largest energy producer and consumer worldwide. Accordingly, the Chinese authorities enacted the Resource Tax Law (RTL) on August 26, 2019. Whether such a policy can achieve its desired goals has not been verified. Thus, our study applies a difference-in-differences approach to examine the effect of RTL on a firm's energy efficiency (EE) with panel data of A-shares from 2017 to 2022. Benchmark analysis and robustness tests demonstrate RTL's significant effect on resource-based firm's EE. Additional tests deeply explore the policy effect under different situations. First, RTL boosts more for firms with higher initial EE levels than those with lower levels. Second, RTL enhances EE by promoting firms' research and development investments, confirming the existence of the Porter hypothesis in China. Third, this policy shows a stronger positive effect on firms with political connections than those without. Finally, we detect that RTL has no significant impact in the western region, while its effect is significantly stronger in the middle region compared to the eastern region. Our empirical findings suggest that corporate firms and the government should adopt appropriate actions to enhance RTL's policy effect.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0323668"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gait assessment using a 2D video-based motion analysis app in healthy subjects and subjects with lower limb amputation - A pilot study.","authors":"Frithjof Doerks, Fenna Harms, Michael Schwarze, Eike Jakubowitz, Bastian Welke","doi":"10.1371/journal.pone.0324499","DOIUrl":"https://doi.org/10.1371/journal.pone.0324499","url":null,"abstract":"<p><strong>Introduction: </strong>Although three-dimensional marker-based motion analysis is the gold standard for biomechanical investigations, it is time-consuming and cost-intensive. The conjunction of monocular video recordings with pose estimation algorithms addresses this gap. With the Orthelligent VISION app (OPED GmbH) a commercial and easy-to-use tool is now available for implementation in everyday clinical practice. The study investigates the accuracy of the 2D video-based system in measuring joint kinematics, expressed as range of motion, compared to an optoelectronic 3D motion analysis system as the gold standard.</p><p><strong>Materials and methods: </strong>Its accuracy was determined by synchronously measuring ten healthy subjects with Orthelligent and the optoelectronic 3D motion analysis system Qualisys (Qualisys AB) during level walking and at different treadmill walking speeds (1 m/s; 1.4 m/s; 1.8 m/s). Range of motion (RoM) of lower limb joints and time-distance parameters were compared using Bland-Altman plots, t-tests, and correlations between systems. Kinematic outputs of two subjects with a lower limb amputation were also analyzed.</p><p><strong>Results: </strong>The mean RoM deviation was smaller for the knee (3.8°) and hip joints (3.7°) than for the ankle joint (5.4°), but differed significantly between systems in most conditions. The correlation range was 0.36 ≤ r ≤ 0.83, with best results for 1 m/s treadmill walking (mean r = 0.71 across joints). While the accuracy was affected by high inter-subject variability, individual RoM changes from slow to fast walking did not differ between the systems. The kinematics of the prosthetic and sound leg of individuals with an amputation exhibited characteristic patterns in the video-based system, even though side differences were smaller compared to the optoelectronic measurement.</p><p><strong>Conclusions: </strong>The rather high inter-subject variability would make future comparisons between individuals challenging. Nonetheless, the app shows potential for intra-subject progress monitoring.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0324499"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycaemic level and glycaemic variability in acute ischaemic stroke and functional outcome: An observational continuous glucose monitoring study.","authors":"Lukana Preechasuk, Siân Rilstone, Wen Xi Tang, Jackie Man, Mingming Yang, Erica Zhao, Lily Hoque, Elif Tuncay, Peter Wilding, Ian Godsland, Omid Halse, Soma Banerjee, Nick Oliver, Neil E Hill","doi":"10.1371/journal.pone.0318456","DOIUrl":"https://doi.org/10.1371/journal.pone.0318456","url":null,"abstract":"<p><strong>Introduction: </strong>Glycaemic variability has been associated with poor outcomes in critically ill patients. We aimed to study the association between glycaemic variability and functional outcome in patients with acute ischaemic stroke using continuous glucose monitoring to ensure all episodes of hyper- and hypoglycaemia were captured.</p><p><strong>Research design and methods: </strong>Participants with acute ischaemic stroke were enrolled and started blinded continuous glucose monitoring (Dexcom G6) between November 2020 and December 2022. Glucose data from the first 72 hours after admission were analysed. Patients were classified into 3 groups based on change in functional status (Modified Rankin Scale) between admission and discharge. These included (i) remained independent (RI); (ii) deteriorated to dependent (DD); and (iii) remained dependent (RD).</p><p><strong>Results: </strong>Data of 67 patients (mean±SD age 72.1 ± 14 years) were analysed; 19 participants had diabetes. The median (IQR) National Institutes of Health Stroke Scale (NIHSS) was 8 (3,14), and 34.3% received reperfusion therapy. The percentage of patients with RI, DD, and RD was 25.4, 55.2, 19.4%. Patients with DD had older age, higher rate of atrial fibrillation, systolic blood pressure, rate of in-hospital infection, NIHSS at admission and at 24 hours after reperfusion therapy compared to those RI. Continuous glucose monitoring was started at 38.4 (29.5,51) hours after stroke onset. Those with DD had higher mean glucose, %time above 180 mg/dL, and glucose standard deviation than the RI group at discharge. Multivariate analysis showed only an association between NIHSS at admission and deterioration in functional status.</p><p><strong>Conclusions: </strong>In this pilot study, an association between glycaemic variability and functional deterioration after acute ischaemic stroke was not observed. Clinical Trial Registration numberNCT04521634.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0318456"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Housing prices in China follow Zipf's law.","authors":"Yalin He, Bailin Zheng, Yue Kai","doi":"10.1371/journal.pone.0324239","DOIUrl":"https://doi.org/10.1371/journal.pone.0324239","url":null,"abstract":"<p><p>This study verifies that housing prices in China follow Zipf's law by analyzing the average housing prices in 200 Chinese cities between 2015 and 2023. The Kolmogorov-Smirnov (KS) test further supports this conclusion. Furthermore, the study explores housing price trends in different city tiers and uses rank clocks to reveal price fluctuations, which is not captured by the Zipf's law. Using nine-year housing price time series data, the ARIMA and ConvLSTM models generate short-term forecasts. The forecasting results are evaluated using various indicators, and KS tests on the predicted prices show a better conformity to Zipf's law. This paper also builds a dynamic housing price model, offering a new perspective to understand and predict housing price trends. The results of this paper can shed light on the real estate market.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0324239"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of living kidney donation on blood pressure and arterial stiffness: Systematic review and meta-analysis.","authors":"Maria Smyrli, Theodora Oikonomaki, Chrysanthi Skalioti, Eleni Kapsia, Ioannis N Boletis, Smaragdi Marinaki","doi":"10.1371/journal.pone.0325390","DOIUrl":"https://doi.org/10.1371/journal.pone.0325390","url":null,"abstract":"<p><strong>Background: </strong>We aimed to conduct a systematic review and meta-analysis (PROSPERO CRD42023480478) regarding the impact of kidney donation on arterial stiffness indices such as pulse wave velocity (PWV) and augmentation index (AIx), along with its effect on blood pressure.</p><p><strong>Methods: </strong>We searched for publications related to kidney/renal donors, arterial stiffness, blood pressure, and cardiovascular risk, and included every study employing those terms. A p-value < 0.05 was considered statistically significant.</p><p><strong>Results: </strong>Twelve studies and 2059 individuals, with a mean age of 46.53 ± 11.27 years, were included in the analysis. Male donors constituted 40.6% of the participants, and the mean follow-up was 2.62 ± 3.2 years. Eleven studies indicated that systolic (SBP) and diastolic blood pressure remained stable within the first year after nephrectomy. However, as the follow-up period extended, especially beyond one year, both were increased (median difference (MD) of SBP was 2.09 [0.06, 4.12] over the first year and 7.7 [6.96, 8.44] over the 5 years of follow-up). Regarding PWV and AIx, assessed in 6 studies, no fluctuations were observed post-donation (MD of PWV was 0.1620 [-0.0423; 0.3662], and MD of AIx was 8.2265 [3.6450; 12.8080]). In addition, 11 studies revealed a decline in the estimated glomerular filtration rate (eGFR) after nephrectomy (MD -27.4960, p-value < 0.0001), though no albuminuria was observed. Lastly, BMI demonstrated negligible changes throughout the follow-up.</p><p><strong>Conclusion: </strong>Kidney donation is a relatively safe procedure, and despite the observed decline in eGFR, it does not per se impose further cardiovascular burden on the donors. However, the heterogeneity and the lack of data underscores the need for high-quality studies so as to elucidate the connection between arterial stiffness, blood pressure, and GFR level.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0325390"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of study shift on the interrelationships among chronobiological factors, health practices, and anthropometry in adolescents.","authors":"Raphael Corrêa Martins, Flávia Dos Santos Barbosa Brito, Cintia Chaves Curioni","doi":"10.1371/journal.pone.0322617","DOIUrl":"https://doi.org/10.1371/journal.pone.0322617","url":null,"abstract":"<p><p>The mismatch between chronobiological predispositions and social demands makes it difficult for adolescents with an evening chronotype to maintain healthy habits. This study aimed to explore the interrelationships among chronobiological factors, health practices, and anthropometry in adolescents attending morning and afternoon class shifts. In this cross-sectional study, 925 adolescents (14-19 years old) completed an online questionnaire covering food practices, chronotype, sleep duration, social jetlag, screen time, socioeconomic data, physical activity, school shift, and anthropometric measurements. The interrelationships among these variables were analyzed through structural equation modeling using six path models. Food practices were categorized into \"complete meals\" (breakfast, lunch, dinner, and bean consumption) - as healthy practices, \"unhealthy foods\" (sweets, fried snacks, soft drinks, salty ultra-processed foods, and fast food) and \"vegetables and fruits,\" stratified by shift (morning or afternoon), with body mass index by age (BMI/age) as the main outcome. Among students in the morning shift, reduced sleep time was directly associated with increased BMI/age, while longer screen time was negatively associated with complete meal practices and physical activity. In the afternoon shift, a greater tendency towards an evening chronotype among adolescents was associated with reduced complete meal practices and lower regular fruit and vegetable consumption. Sleep duration was positively associated with a greater likelihood of a complete meal practices and regular fruit consumption. Moreover, longer screen time was positively associated with unhealthy food consumption. In both shifts, physically active adolescents consumed fruits more regularly. The practice of regular consumption of complete meals, fruits, vegetables, or unhealthy foods, in addition to being directly influenced by chronotype and other variables, was also influenced by shift. Adolescents with shorter sleep duration were at a higher risk of being overweight, suggesting a direct influence of sleep on anthropometric measurements. Our findings underscore the importance of considering study shifts in future interventions.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 5","pages":"e0322617"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}