PLoS ONE最新文献

{"title":"Akt3 links mitochondrial function to the regulation of Aurora B and mitotic fidelity.","authors":"Zachary J Hough, Fatemeh Nasehi, Daniel G Corum, Russell A Norris, Ann C Foley, Robin C Muise-Helmericks","doi":"10.1371/journal.pone.0315751","DOIUrl":"10.1371/journal.pone.0315751","url":null,"abstract":"<p><p>Akt3 is a key regulator of mitochondrial homeostasis in the endothelium. Akt3 depletion results in mitochondrial dysfunction, decreased mitochondrial biogenesis, and decreased angiogenesis. Here we link mitochondrial homeostasis with mitotic fidelity-depletion of Akt3 results in the missegregation of chromosomes as visualized by multinucleation and micronuclei formation. We have connected Akt3 to Aurora B, a significant player in chromosome segregation. Akt3 localizes to the nucleus, where it associates with and regulates WDR12. During mitosis, WDR12 is localized to the dividing chromosomes, and its depletion results in a similar mitotic phenotype to Akt3 depletion. WDR12 associates with Aurora B, both of which are downregulated under conditions of Akt3 depletion. We used the model oxidant paraquat to induce mitochondrial dysfunction to test whether the Akt3-dependent effect on mitochondrial homeostasis is linked to mitotic function. Paraquat treatment also causes chromosome missegregation by inhibiting the expression of Akt3, WDR12, and Aurora B. The inhibition of ROS rescued both the mitotic fidelity and the expression of Akt3 and Aurora B. Akt3 directly phosphorylates the major nuclear export protein CRM-1, causing an increase in its expression, resulting in the inhibition of PGC-1 nuclear localization, the master regulator of mitochondrial biogenesis. The Akt3/Aurora B pathway is also dependent on CRM-1. CRM-1 overexpression resulted in chromosome missegregation and downregulation of Aurora B similar to that of Akt3 depletion. Akt3 null hearts at midgestation (E14.5), a stage in which proliferation is occurring, have decreased Aurora B expression, increased CRM-1 expression, decreased proliferation, and increased apoptosis. Akt3 null hearts are smaller and have a thinner compact cell layer than age-matched wild-type mice. Akt3 null tissue has dysmorphic nuclear structures, suggesting mitotic catastrophe. Our findings show that mitochondrial dysfunction induced by paraquat or Akt3 depletion results in a CRM-1-dependent disruption of Aurora B and mitotic fidelity.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0315751"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Potential model of Scalesia pedunculata carbon sequestration through restoration efforts in agricultural fields of Galapagos.","authors":"Nicolás Velasco, Patricia Jaramillo Diaz","doi":"10.1371/journal.pone.0319749","DOIUrl":"10.1371/journal.pone.0319749","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1371/journal.pone.0302680.].</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0319749"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping the value of commercial fishing and potential costs of offshore wind energy on the U.S: West Coast: Towards an assessment of resource use tradeoffs.","authors":"Blake E Feist, Robert Griffin, Jameal F Samhouri, Leena Riekkola, Andrew O Shelton, Y Allen Chen, Kayleigh Somers, Kelly Andrews, Owen R Liu, Jennifer Ise","doi":"10.1371/journal.pone.0315319","DOIUrl":"10.1371/journal.pone.0315319","url":null,"abstract":"<p><p>The West Coast of the U.S. has a vast offshore wind energy (OWE) electricity generation potential with value on the order of billions of USD, and pressure is mounting to develop large OWE projects. However, this seascape has numerous existing resource extraction uses, including a multi-billion dollar commercial fishing industry, which create the potential for conflict. To date, spatially explicit comparisons of OWE and commercial fisheries value have not been done, but are essential for marine spatial planning and for investigating the tradeoffs of OWE development on existing marine uses. In this analysis, we generate maps of OWE levelized cost of energy and of total economic activity generated by the top eight commercial fishing targets that account for the vast majority (~84%) of landed revenue off the U.S. West Coast. We quantify spatial overlap between these two ocean uses and use multiobjective optimization to develop tradeoff frontiers to investigate implications for both sectors from established state goals or mandates for OWE power generation capacity. There are clear differences in the exposure of each fishery in their traditional fishing grounds as a function of differing OWE capacity goals and outcomes vary depending on whether OWE development goals are achieved at a state-by-state level or a region-wide level. Responsible siting of OWE projects includes careful consideration of existing commercial fishing activities, and responsible transition to renewable energies on the West Coast and elsewhere accounts for the socio-economic consequences of the total economic activity associated with each fishery.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0315319"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of novel antiviral host factors by functional gene expression analysis using in vitro HBV infection assay systems.","authors":"Takuto Nosaka, Tatsushi Naito, Yu Akazawa, Kazuto Takahashi, Hidetaka Matsuda, Masahiro Ohtani, Tsutomu Nishizawa, Hiroaki Okamoto, Yasunari Nakamoto","doi":"10.1371/journal.pone.0314581","DOIUrl":"10.1371/journal.pone.0314581","url":null,"abstract":"<p><p>To cure hepatitis B virus (HBV) infection, it is essential to elucidate the function of hepatocyte host factors in regulating the viral life cycle. Signaling and transcription activator of transcription (STAT)1 play important roles in immune responses, but STAT1-independent pathways have also been shown to have important biological reactivity. Using an in vitro HBV infection assay system, the current study aimed to investigate the STAT1-independent host factors that contribute to the control of viral infection by comprehensive functional screening. The in vitro HBV infection system was established using primary human hepatocytes (PXB cells) infected with HBV derived from a plasmid containing the 1.3-mer HBV genome. Comprehensive functional studies were performed using small interfering RNA (siRNA) and vector transfection and analyzed using microarrays. Knockdown of STAT1 increased viral products in HBV-transfected HepG2 cells, but decreased in HBV-infected PXB cells. RNA microarray was performed using HBV-infected PXB cells with STAT1 knockdown. Fumarylacetoacetate hydrolase (FAH) was extracted by siRNA of genes in PXB cells altered by STAT1 knockdown. Transfection of FAH inhibited HBV replication. Dimethyl fumarate (DMF), the methyl ester of FAH metabolite, showed antiviral effects by inducing autophagy and anti-HBV-related genes. Independently of STAT1, FAH was identified as a host factor that contributes to the control of viral infection, and its metabolite, DMF, exhibited antiviral activity. These results suggest that the novel host factor FAH and its metabolites may be an innovative therapeutic strategy to control the HBV life cycle.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0314581"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An optimized machine learning framework for predicting and interpreting corporate ESG greenwashing behavior.","authors":"Fanlong Zeng, Jintao Wang, Chaoyan Zeng","doi":"10.1371/journal.pone.0316287","DOIUrl":"10.1371/journal.pone.0316287","url":null,"abstract":"<p><p>The accurate prediction and interpretation of corporate Environmental, Social, and Governance (ESG) greenwashing behavior is crucial for enhancing information transparency and improving regulatory effectiveness. This paper addresses the limitations in hyperparameter optimization and interpretability of existing prediction models by introducing an optimized machine learning framework. The framework integrates an Improved Hunter-Prey Optimization (IHPO) algorithm, an eXtreme Gradient Boosting (XGBoost) model, and SHapley Additive exPlanations (SHAP) theory to predict and interpret corporate ESG greenwashing behavior. Initially, a comprehensive ESG greenwashing prediction dataset was developed through an extensive literature review and expert interviews. The IHPO algorithm was then employed to optimize the hyperparameters of the XGBoost model, forming an IHPO-XGBoost ensemble learning model for predicting corporate ESG greenwashing behavior. Finally, SHAP was used to interpret the model's prediction outcomes. The results demonstrate that the IHPO-XGBoost model achieves outstanding performance in predicting corporate ESG greenwashing, with R², RMSE, MAE, and adjusted R² values of 0.9790, 0.1376, 0.1000, and 0.9785, respectively. Compared to traditional HPO-XGBoost models and XGBoost models combined with other optimization algorithms, the IHPO-XGBoost model exhibits superior overall performance. The interpretability analysis using SHAP theory highlights the key features influencing the prediction outcomes, revealing the specific contributions of feature interactions and the impacts of individual sample features. The findings provide valuable insights for regulators and investors to more effectively identify and assess potential corporate ESG greenwashing behavior, thereby enhancing regulatory efficiency and investment decision-making.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0316287"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conservative endometrioma surgery: The combined technique versus CO2-laser vaporization only (BLAST: Belgium LAser STudy): Clinical protocol for a multicenter randomized controlled trial.","authors":"Celine Bafort, Sharon Lie Fong, Steffen Fieuws, Brecht Geysenbergh, Michelle Nisolle, Jean-Luc Squifflet, Linda Tebache, Christine Wyns, Christel Meuleman, Carla Tomassetti","doi":"10.1371/journal.pone.0315709","DOIUrl":"10.1371/journal.pone.0315709","url":null,"abstract":"<p><strong>Background: </strong>The surgical management of endometrioma(s) remains challenging. Although laparoscopic surgery is a well-established treatment of endometrioma(s), caution is required to minimize ovarian damage. Several surgical techniques have been described to treat endometrioma(s): classical cystectomy, ablative techniques, or a combination of both. As cystectomy is strongly associated with a reduction in ovarian reserve, this randomized controlled trial (RCT) aims to determine to what extent the two other surgical procedures may affect ovarian reserve by comparing changes in serum anti-Müllerian hormone (AMH) levels concentrations after each type of surgery.</p><p><strong>Methods: </strong>This is a multicenter, non-blinded, RCT with parallel groups (group 1 (combined technique) versus group 2 (CO2 laser vaporization only)) and allocation 1:1. Four Belgian centers will be involved. Main inclusion criteria are symptomatic patients (pain and/or infertility), 18-40 years (both inclusive) with an endometriotic cyst (mean diameter of ≥ 2.5 cm and ≤ 8 cm) and AMH level ≥ 0.7 ng/mL. Suspicion of malignancy, a contralateral endometrioma of > 2 cm, use of gonadotrophin-releasing hormone (GnRH) analogues around timing of surgery or previous oophorectomy are exclusion criteria. The primary aim is the evaluation of the difference in serum AMH levels between baseline and 3 months postoperatively (or delta AMH). The secondary outcomes include differences in AMH levels at 6 and 12 months postoperatively, cyst recurrence rate, evolution of pain pattern and fertility outcomes.</p><p><strong>Discussion: </strong>The present study will help us to answer the question on which surgical technique for endometrioma(s) has the most favorable outcome in patients wishing to preserve their reproductive potential.</p><p><strong>Trial registration: </strong>ClinicalTrials.gov: NCT04151433. Registered on November 5th, 2019.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0315709"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Efficacy of probiotic supplements in the treatment of sarcopenia: A systematic review and meta-analysis.","authors":"Yi Wang, Ping Lei","doi":"10.1371/journal.pone.0320199","DOIUrl":"10.1371/journal.pone.0320199","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1371/journal.pone.0317699.].</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0320199"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: National income accounting attributes and economic welfare. Evidence from Pakistan.","authors":"Shuang Yang, Muhammad Waris, Muhammad Kashif Nawaz, Cheng Chen, Ijaz Younis","doi":"10.1371/journal.pone.0320259","DOIUrl":"10.1371/journal.pone.0320259","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1371/journal.pone.0301829.].</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0320259"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Energy evolution and damage ontology modeling of coal destruction at different water contents.","authors":"Yongjiang Yu, Jiaming Liu, Wenjing Guo, Zhiyuan Song, Yuntao Yang, Shangqing Zhao, Dong Xu, Zhiqiang Wu","doi":"10.1371/journal.pone.0316941","DOIUrl":"10.1371/journal.pone.0316941","url":null,"abstract":"<p><p>The aim of this study was to investigate the energy evolution characteristics and an ontological model of the deformation of coal under different water contents. Uniaxial compression tests were conducted for coal with different water contents, and the analyses were based on the energy principle and the principle of minimum energy dissipation. The results showed that the physical properties of the coal specimens were different under different water contents, the peak strain was positively correlated with water content, and the compressive strength and elastic modulus were negatively correlated with water content. Additionally, the compressive strength and elastic modulus of the coal specimens showed a steep and subsequent slow-change trend. From an energy perspective, the higher the water content of the coal specimens, the higher their energy dissipation at the peak; the smaller the limiting elastic strain energy, the lower the absorbed energy. The principle of minimum energy dissipation was used to deduce the energy evolution and mechanical properties of coal body damage under different water contents, deriving the initial and critical values of damage. The water content of the coal specimens was positively correlated with their initial and critical values of damage, and the relationship with water content was nonlinear. This result was used to establish a stress-strain ontology model for coal rocks with different water contents under uniaxial compression. The model is an improvement over traditional ontology models, addressing the problem of low accuracy in simulations of materials at the compaction stage.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0316941"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring bioactive compound origins: Profiling gene cluster signatures related to biosynthesis in microbiomes of Sof Umer Cave, Ethiopia.","authors":"Abu Feyisa Meka, Gessesse Kebede Bekele, Musin Kelel Abas, Mesfin Tafesse Gemeda","doi":"10.1371/journal.pone.0315536","DOIUrl":"10.1371/journal.pone.0315536","url":null,"abstract":"<p><p>Sof Umer Cave is an unexplored extreme environment that hosts novel microbes and potential genetic resources. Microbiomes from caves have been genetically adapted to produce various bioactive metabolites, allowing them to survive and tolerate harsh conditions. However, the biosynthesis-related gene cluster signatures in the microbiomes of Sof Umer Cave have not been explored. Therefore, high-throughput shotgun sequencing was used to explore biosynthesis-related gene clusters (BGCs) in the microbiomes of Sof Umer Cave. The GeneAll DNA Soil Mini Kit was used to extract high-molecular-weight DNA from homogenized samples, and the purified DNA was sequenced using a NovaSeq PE150. According to the Micro-RN database, the most common microbial genera in Sof Umer Cave are Protobacteria, Actinobacteria, Verrucomicrobiota, and Cyanobacteria. The biosynthesis-related gene clusters were annotated and classified, and the BGCs were predicted using antiSMASH and NAPDOS1. A total of 460 putative regions of BGCs encoding a wide range of secondary metabolites were identified, including RiPP (47.82%), terpene (19.57%), NRPS (13.04%), hybrid (2.18%), and other newly annotated (10.87%) compounds. Additionally, the NAPDOS pipeline identified a calcium-dependent antibiotic gene cluster from Streptomyces coelicolor, an actinomycin gene cluster from Streptomyces chrysomallus, and a bleomycin gene cluster from Streptomyces verticillus. These findings highlight the untapped biosynthetic potential of the Sof Umer Cave microbiome, as well as its potential for the discovery of natural products.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 3","pages":"e0315536"},"PeriodicalIF":2.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}