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High prevalence of poor sleep quality and sleep deficit: A study in children, adolescents, and adult soccer players. 睡眠质量差和睡眠不足的高患病率:一项针对儿童、青少年和成年足球运动员的研究。
IF 2.6 3区 综合性期刊
PLoS ONE Pub Date : 2025-10-10 eCollection Date: 2025-01-01 DOI: 10.1371/journal.pone.0333774
Lúcio A Cunha, Elisa A Marques, João Brito, Michele Lastella, Pedro Figueiredo
{"title":"High prevalence of poor sleep quality and sleep deficit: A study in children, adolescents, and adult soccer players.","authors":"Lúcio A Cunha, Elisa A Marques, João Brito, Michele Lastella, Pedro Figueiredo","doi":"10.1371/journal.pone.0333774","DOIUrl":"https://doi.org/10.1371/journal.pone.0333774","url":null,"abstract":"<p><strong>Objective: </strong>This observational study aimed to provide insight into the sleep behaviors, chronotypes, and sleep needs of athletes by examining children, adolescents, and adult soccer players from different competitive levels.</p><p><strong>Methods: </strong>The study included 864 soccer players (n = 747 males) of various age groups [median age: 17 (interquartile range: 14-20)]. The participants completed an online questionnaire that included demographic questions, the Pittsburgh Sleep Quality Index (PSQI), the Epworth Sleepiness Scale (ESS), the Morningness-Eveningness Questionnaire (MEQ), and a question about their sleep needs.</p><p><strong>Results: </strong>Adult players had a higher prevalence of poor sleep quality, excessive daytime sleepiness, and sleep deficit than children and teenagers (p < 0.001). Lower sleep duration was associated with poorer sleep quality and excessive daytime sleepiness (rs = -0.59 to -0.17, p < 0.01). Sleep needs were significantly higher than the habitual sleep duration in teenagers (42 min, p < 0.001) and adult players (41 min, p < 0.001). Players that had poor sleep quality (OR = 3.98, 95% CI: 2.78-5.77), excessive daytime sleepiness (OR = 3.72, 95% CI: 2.32-6.1), evening chronotype (OR = 2.54, 95% CI: 1.48-4.40), and later ending time of training (OR = 1.12, 95% CI: 1.07-1.19) could be at a higher risk of having sleep deficit.</p><p><strong>Conclusion: </strong>Nearly half of the adult players had poor sleep quality. Additionally, one-third of the children, and almost half of the teenagers and adult players, had a sleep deficit. Players with poor sleep quality, an evening chronotype, excessive daytime sleepiness, and a later training time might be at a higher risk of experiencing a sleep deficit.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 10","pages":"e0333774"},"PeriodicalIF":2.6,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12513668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145275538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Uncovering key determinants of well-being among older Canadian retirees in New Brunswick: Protocol for a mixed-methods study. 揭示新不伦瑞克省加拿大老年退休人员幸福感的关键决定因素:混合方法研究的协议。
IF 2.6 3区 综合性期刊
PLoS ONE Pub Date : 2025-10-10 eCollection Date: 2025-01-01 DOI: 10.1371/journal.pone.0334022
Yasin M Yasin, Areej Al-Hamad, Kateryna Metersky, Emily Richard
{"title":"Uncovering key determinants of well-being among older Canadian retirees in New Brunswick: Protocol for a mixed-methods study.","authors":"Yasin M Yasin, Areej Al-Hamad, Kateryna Metersky, Emily Richard","doi":"10.1371/journal.pone.0334022","DOIUrl":"https://doi.org/10.1371/journal.pone.0334022","url":null,"abstract":"<p><strong>Aims: </strong>To uncover the key determinants of physical, mental, social, financial, and spiritual well-being among older Canadian retirees living in New Brunswick and explore how individual, community, organizational, and societal factors interact to shape healthier, more fulfilling aging.</p><p><strong>Design: </strong>Explanatory sequential mixed-methods study guided by the Socio-Ecological Model.</p><p><strong>Methods: </strong>A cross-sectional survey will be conducted with 600 retirees aged 65 and older using the WISE Scale, a multidimensional measure of well-being. Surveys will be offered online and in person with accessibility supports. Data will be analyzed in SPSS using descriptive statistics and multiple regression. A purposive subsample of 15-25 participants will be invited for semi-structured interviews to enrich understanding of survey findings. Interviews will be thematically analyzed in NVivo, following the four pillars of trustworthiness. Triangulation will integrate quantitative and qualitative findings.</p><p><strong>Discussion: </strong>The study will generate detailed insights into how multiple layers of influence affect older adults' well-being in retirement, addressing critical gaps in research, policy, and practice. Findings will inform tailored community programs, effective strategies for healthcare providers, equitable policies, and age-friendly supports that promote holistic well-being for older retirees in New Brunswick, particularly among English-speaking populations. By identifying specific factors that enhance or hinder well-being, this research will support more responsive and inclusive strategies for healthy aging.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 10","pages":"e0334022"},"PeriodicalIF":2.6,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12513576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145275544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Editorial Note: Undergoing lignin-coated seeds to cold plasma to enhance the growth of wheat seedlings and obtain future outcome under stressed ecosystems. 编者按:将木质素包被的种子导入冷等离子体,以促进小麦幼苗的生长,并获得在逆境生态系统下的未来结果。
IF 2.6 3区 综合性期刊
PLoS ONE Pub Date : 2025-10-10 eCollection Date: 2025-01-01 DOI: 10.1371/journal.pone.0334274
{"title":"Editorial Note: Undergoing lignin-coated seeds to cold plasma to enhance the growth of wheat seedlings and obtain future outcome under stressed ecosystems.","authors":"","doi":"10.1371/journal.pone.0334274","DOIUrl":"https://doi.org/10.1371/journal.pone.0334274","url":null,"abstract":"","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 10","pages":"e0334274"},"PeriodicalIF":2.6,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12513580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145275428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
On the effectiveness of network metrics on key class prediction: An empirical study. 网络指标对关键类别预测有效性的实证研究。
IF 2.6 3区 综合性期刊
PLoS ONE Pub Date : 2025-10-10 eCollection Date: 2025-01-01 DOI: 10.1371/journal.pone.0334408
Shiyuan Zhou, Wei Wu, Jiale Wang, Hongbing Liu, Chenxiang Yuan
{"title":"On the effectiveness of network metrics on key class prediction: An empirical study.","authors":"Shiyuan Zhou, Wei Wu, Jiale Wang, Hongbing Liu, Chenxiang Yuan","doi":"10.1371/journal.pone.0334408","DOIUrl":"https://doi.org/10.1371/journal.pone.0334408","url":null,"abstract":"<p><p>Key classes are the most important classes in a software system, which provide an excellent foundation for developers-especially those new to the field-to understand unfamiliar software systems. In the past decade, several key class prediction (KCP) approaches have been proposed. They used design metrics extracted from source code and unweighted network metrics computed on class coupling networks as features and built machine-learning models to predict whether a class is a key class or not. However, previous studies mainly focused on improving the performance of KCP models in the within-project (i.e., KCP in the same project) context, and the network metrics they used are unweighted and inaccurate, as they are computed on unweighted and incomplete class coupling networks. These limitations lead to a lack of thorough evaluation of the effectiveness of network metrics for KCP, especially in the cross-project (KCP across diverse projects) context, which in turn results in uncertainty about how to choose suitable metrics as features when building KCP models. To fill this gap, in this paper, we thoroughly evaluate the effectiveness of network metrics for KCP. Specifically, we build weighted and more complete class coupling networks for software, and introduce a set of weighted network metrics to characterize class complexity. Then, we build different KCP models using the Random Forest learner and the Naive Bayes model for two KCP contexts (i.e., within-project and cross-project), respectively, with design metrics, unweighted/weighted network metrics, and their combinations being features. Finally, through an empirical study on 18 open-source Java projects, we thoroughly investigate the relative effectiveness of network metrics over design metrics across the two KCP contexts. Our results suggest that when building KCP models, to achieve better performance, researchers and practitioners should consider using unweighted (or weighted) network metrics alone or along with design metrics in the within-project KCP context, using design metrics alone or along with unweighted (or weighted) network metrics in the cross-project KCP context, and using unweighted (or weighted) network metrics along with design metrics across the two KCP contexts.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 10","pages":"e0334408"},"PeriodicalIF":2.6,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12513632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145275508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Predictive value of urinary [TIMP-2]•[IGFBP7] for AKI among sepsis, stroke, and cardiac surgery cohorts: A prospective study. 尿[TIMP-2]•[IGFBP7]在败血症、卒中和心脏手术队列中对AKI的预测价值:一项前瞻性研究
IF 2.6 3区 综合性期刊
PLoS ONE Pub Date : 2025-10-10 eCollection Date: 2025-01-01 DOI: 10.1371/journal.pone.0332272
Chaoyun Jiang, Cheng Yang, Hui Chen, Xiaofang Jiang, Jiahao Zhang, Juan Felipe Alvarez, Haichuan Yu, Yao Zhu, Lianjiu Su, Zhiyong Peng
{"title":"Predictive value of urinary [TIMP-2]•[IGFBP7] for AKI among sepsis, stroke, and cardiac surgery cohorts: A prospective study.","authors":"Chaoyun Jiang, Cheng Yang, Hui Chen, Xiaofang Jiang, Jiahao Zhang, Juan Felipe Alvarez, Haichuan Yu, Yao Zhu, Lianjiu Su, Zhiyong Peng","doi":"10.1371/journal.pone.0332272","DOIUrl":"https://doi.org/10.1371/journal.pone.0332272","url":null,"abstract":"<p><strong>Background: </strong>TIMP-2 and IGFBP7 have shown effectiveness as biomarkers for predicting Acute Kidney Injury (AKI). However, the variations in the predictive capacity of urinary [TIMP-2]• [IGFBP7] for AKI across different etiologies remain unexplored. This study aimed to assess the predictive capability of urinary [TIMP-2]• [IGFBP7] for AKI in three distinct disease cohorts (stroke, sepsis, and cardiac surgery) characterized by differing AKI etiologies.</p><p><strong>Methods: </strong>This prospective observational study evaluated the predictive value of urinary [TIMP-2]• [IGFBP7] among three cohorts with varying AKI causes. Binary logistic regression was employed to identify AKI's independent risk factors and develop a combined prediction model. The predictive value was assessed using Receiver Operating Characteristic (ROC) curves and Area Under the Curve (AUC) analyses.</p><p><strong>Results: </strong>337 patients were included in the final analysis, with 109 (32.3%) developing AKI. AKI occurred in 39 (22.2%) stroke patients, 52 (50%) sepsis patients, and 18 (31.6%) post-cardiac surgery patients. [TIMP-2]• [IGFBP7] exhibited predictive value for AKI with an AUC of 0.86 (95% CI 0.75-0.90) in stroke, 0.82 (95% CI 0.74-0.91) in sepsis, and 0.90 (95% CI 0.82-0.98) in post-cardiac surgery. DeLong's test indicated no significant differences in the predictive value of [TIMP-2]• [IGFBP7] between the cardiac surgery group and the stroke (P=0.20) and sepsis (P=0.21) groups.</p><p><strong>Conclusion: </strong>The combined prediction model, which integrates urinary [TIMP-2]• [IGFBP7] concentrations and AKI risk factors, significantly enhances AKI prediction. No significant differences were found in the predictive value of urinary [TIMP-2]• [IGFBP7] for AKI among the stroke, sepsis, and cardiac surgery cohorts.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 10","pages":"e0332272"},"PeriodicalIF":2.6,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12513614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145275517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High variation in the gluten composition and grain protein content among synthetic wheat lines. 合成小麦品系间面筋成分和籽粒蛋白质含量差异较大。
IF 2.6 3区 综合性期刊
PLoS ONE Pub Date : 2025-10-10 eCollection Date: 2025-01-01 DOI: 10.1371/journal.pone.0331619
Rahman Ebrahimzadegan, Ali Sanati, Ghader Mirzaghaderi
{"title":"High variation in the gluten composition and grain protein content among synthetic wheat lines.","authors":"Rahman Ebrahimzadegan, Ali Sanati, Ghader Mirzaghaderi","doi":"10.1371/journal.pone.0331619","DOIUrl":"https://doi.org/10.1371/journal.pone.0331619","url":null,"abstract":"<p><p>Wheat wild relatives are important sources for the genetic enhancement of cultivated wheat. Here, we evaluated the gluten composition, grain protein content, and several quality-related gluten indices across 47 synthetic wheat lines or amphiploids resulted from the crosses between emmer wheat, durum wheat, T. timopheevii, Ae. crassa, Ae. ventricosa and Ae. tauschii. The grain protein content ranged from 15% to 23.5%, in 79% of the studied lines. Lines exhibiting high protein contents generally demonstrated normal gluten strength. This characteristic primarily resulted from the inclusion of emmer wheat, durum wheat, or T. timopheevii as one of the parental lines in their pedigree. About 18% of the lines, which mainly resulted from (T. durum × Ae. tauschii) × common wheat crosses demonstrated strong gluten properties. The analysis of high molecular weight glutenin subunits (HMW-GSs) revealed a greater diversity for the Glu-B1 locus than those from Glu-A1 and Glu-D1. The most frequently identified HMW-GSs included Null, 1, and 2* at the Glu-A1 locus; 21 + 19, 7 + 8, 14 + 15, 6 + 8, 14 + 18, 21 + 15, 13 + 16 + 9, and 6 + 22 at the Glu-B1; and 3 + 10 or 3 + 10.5, 2 + 12 or 2 + 12.5, and 5 + 10 or 5 + 10.5 at the Glu-D1. Subunits associated with the bread-making quality of wheat, particularly observed in durum wheat × Ae. tauschii cross combinations. Cluster analysis based on gliadin and glutenin subunits did not accurately reflect the genomic composition of the lines, though some lines with similar genomic backgrounds were clustered together. These results suggest the potential of our synthetic wheat lines to enhance the nutritional and baking quality of wheat flour.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 10","pages":"e0331619"},"PeriodicalIF":2.6,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12513617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145275531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tracing Neanderthal mobility through the Romanian Carpathians: A GIS-based least-cost connectivity model. 通过罗马尼亚喀尔巴阡山脉追踪尼安德特人的流动性:基于gis的最低成本连接模型。
IF 2.6 3区 综合性期刊
PLoS ONE Pub Date : 2025-10-10 eCollection Date: 2025-01-01 DOI: 10.1371/journal.pone.0334149
George Murătoreanu, Marian Cosac
{"title":"Tracing Neanderthal mobility through the Romanian Carpathians: A GIS-based least-cost connectivity model.","authors":"George Murătoreanu, Marian Cosac","doi":"10.1371/journal.pone.0334149","DOIUrl":"https://doi.org/10.1371/journal.pone.0334149","url":null,"abstract":"<p><p>Out of the 12,300 known caves within the Romanian Carpathian and Dobrudja mountain area, only 16 have been the focus of consistent archaeological researches which revealed traces of human activity (lithic artefacts, faunal material, combustion areas), roughly consistent with a Middle Palaeolithic technological and/or chronological background. Establishing natural areas connected in terms of accessibility to these caves may offer a different perspective for future researches and, above all, may increase the chances of discovering new caves with traces of human activity. The present study aims to integrate advanced spatial analysis methods, such as Linkage Mapper and Circuitscape, to assess the potential connectivity of these sites. The two models were developed by researchers in the field of biology and belong to the field of deterministic spatial modeling and algorithm-based geospatial analysis. Following the application of these models, we identified the areas of influence of the 16 caves, determined the least-cost paths between them and the main natural obstacles, in order to model spatial connectivity and identify new possible sites along these routes.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 10","pages":"e0334149"},"PeriodicalIF":2.6,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12513586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145275564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigating genetic links of vitamin D metabolism pathway genes (CYP2R1, CYP27B1, CYP24A1, and DBP) in Multiple Sclerosis patients. 研究多发性硬化症患者维生素D代谢途径基因(CYP2R1、CYP27B1、CYP24A1和DBP)的遗传联系。
IF 2.6 3区 综合性期刊
PLoS ONE Pub Date : 2025-10-10 eCollection Date: 2025-01-01 DOI: 10.1371/journal.pone.0333924
Laith Al-Eitan, Asaad Ataa
{"title":"Investigating genetic links of vitamin D metabolism pathway genes (CYP2R1, CYP27B1, CYP24A1, and DBP) in Multiple Sclerosis patients.","authors":"Laith Al-Eitan, Asaad Ataa","doi":"10.1371/journal.pone.0333924","DOIUrl":"https://doi.org/10.1371/journal.pone.0333924","url":null,"abstract":"<p><p>Multiple sclerosis (MS) is believed to result from a complex interplay of behavioral, genetic, and environmental risk factors. Furthermore, some studies indicated that vitamin D deficiency is linked to the emergence of different diseases, including MS. This study aims to determine the genetic associations between vitamin D metabolism gene polymorphisms and MS susceptibility in the Jordanian community. A total of 388 samples (192 MS patients and 196 controls). Genotypes for CYP2R1 (rs10741657, rs12794714), CYP27B1 (rs10877012), CYP24A1 (rs2248359), and DBP (rs7041, rs4588) were determined by PCR/RFLP assay method. The study revealed a significant association with increased MS risk in SNPs rs10877012 (C/A, P = 0.0002) of the CYP27B1 gene and rs4588 (A/A, P = 0.04) of the DBP gene. Additionally, the haplotypes of the CYP2R1 gene revealed a significant association with MS patients and controls (GG, p = 1e-04; AA, p < 0.0001). Moreover, only a SNP rs4588 of the DBP gene has been significantly associated (P = 0.04) with a clinical phenotype of multiple sclerosis and vitamin D deficiency. Understanding these genetic variations in multiple sclerosis susceptibility genes can help healthcare professionals improve early diagnosis and develop personalized treatment options.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 10","pages":"e0333924"},"PeriodicalIF":2.6,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12513619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145275587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential composition of the pulmonary microbiome in HIV-positive versus HIV-negative patients with Pneumocystis jirovecii. hiv阳性与hiv阴性肺囊虫患者肺微生物组的差异组成
IF 2.6 3区 综合性期刊
PLoS ONE Pub Date : 2025-10-10 eCollection Date: 2025-01-01 DOI: 10.1371/journal.pone.0334220
Chang-Run Zhang, Mei Wang, Lei Wang, Li Liu, Yi-Dan Shen, Rui-Fang Chen, Shuai Ren, Wei-Zhong Jin, Hai-Cheng Tang, Qing-Guo Wu
{"title":"Differential composition of the pulmonary microbiome in HIV-positive versus HIV-negative patients with Pneumocystis jirovecii.","authors":"Chang-Run Zhang, Mei Wang, Lei Wang, Li Liu, Yi-Dan Shen, Rui-Fang Chen, Shuai Ren, Wei-Zhong Jin, Hai-Cheng Tang, Qing-Guo Wu","doi":"10.1371/journal.pone.0334220","DOIUrl":"https://doi.org/10.1371/journal.pone.0334220","url":null,"abstract":"<p><p>Pneumocystis jirovecii is frequently detected in HIV patients and individuals with compromised immune function. The clinical outcomes of these two groups differ significantly, yet the underlying reasons remain unclear, with limited studies addressing this issue. This study investigates the alterations in the pulmonary microbiota of HIV-positive and non-HIV patients following pneumocystis jirovecii infection.Collect bronchoalveolar lavage fluid from patients with HIV and non HIV infected Pneumocystis jirovecii, and compare the differences in pulmonary microbiota between the two groups.In total, 77 patients with pulmonary infection that had next generation sequencing performed on their bronchoalveolar lavage fluid and confirmed pneumocystis jirovecii infection were recruited in our study. Of the 77 patients with pneumocystis jirovecii infection, 52 were infected with HIV, and 25 were uninfected.Our findings indicate that HIV-positive patients exhibit a more diverse microbiota, predominantly characterized by viral co-infections. Specifically, 88.5% of HIV-positive patients experienced viral co-infections, primarily involving herpes viruses, followed by bacterial (61.5%) and fungal (40.4%) co-infections. In contrast, non-HIV patients predominantly exhibited bacterial co-infections (72%), followed by viral (52%) and fungal (36%) co-infections. By analyzing the next generation sequencing data of both groups, we identified statistically significant differences in viral infections (p < 0.001), while no significant differences were observed for bacterial or fungal infections. Furthermore, among the background bacteria detected via next generation sequencing in both patient groups, 22 microbial species were commonly present. Notably, Leptospiral virus, Rosette fungus, and Actinomycetes were detected at higher frequencies in HIV-infected pneumocystis jirovecii patients, with statistically significant differences.Through comparing the pulmonary microbiota profiles of HIV-positive and non-HIV patients post-pneumocystis jirovecii infection, we uncovered distinct differences between the two groups, which may hold implications for guiding subsequent treatment strategies and improving clinical outcomes.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 10","pages":"e0334220"},"PeriodicalIF":2.6,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12513626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145275612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the potential of nest archives for establishing long-term trends in local populations of an Arctic-nesting colonial sea duck. 探索巢穴档案的潜力,以建立北极筑巢殖民地海鸭当地种群的长期趋势。
IF 2.6 3区 综合性期刊
PLoS ONE Pub Date : 2025-10-10 eCollection Date: 2025-01-01 DOI: 10.1371/journal.pone.0332605
Inmaculada Álvarez-Manzaneda, Kathleen M Rühland, Marlo Campbell, Matthew P Duda, Mark L Mallory, Nik Clyde, H Grant Gilchrist, Kathryn E Hargan, John P Smol
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