Physiological Reports最新文献_第8页

The effects of alpha-pinene against paracetamol-induced liver damage in male rats. α-蒎烯对扑热息痛引起的雄性大鼠肝损伤的影响。

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70227

Kaveh Rahimi, Anahita Rezaie, Younes Allahverdi, Parham Shahriari, Mahtab Taheri Mirghaed

{"title":"The effects of alpha-pinene against paracetamol-induced liver damage in male rats.","authors":"Kaveh Rahimi, Anahita Rezaie, Younes Allahverdi, Parham Shahriari, Mahtab Taheri Mirghaed","doi":"10.14814/phy2.70227","DOIUrl":"10.14814/phy2.70227","url":null,"abstract":"This study aims to evaluate the hepatoprotective effect of alpha-pinene against N-acetyl-p-aminophenol, paracetamol, (APA)-induced liver damage in rats. Thirty Wistar rats were divided into five groups (n = 6): Group 1: Normal (control). Group 2: APA 640 mg/kg. Group 3: alpha-pinene 50 mg/kg (APA+ αPi 50 mg/kg). Group 4: alpha-pinene 100 mg/kg (APA+ αPi 100 mg/kg). Group 5: silymarin 50 mg/kg (APA+ SIL). Alpha-pinene or silymarin was orally administered after APA administration for 14 consecutive days. This study investigated liver damage by preparing pathology slides from liver tissue. Levels of AST, ALT, ALP, total bilirubin, total antioxidant capacity (TAC), and total oxidant status (TOS) were measured. Inflammatory factors, including NF-kB gene expression and levels of IL-6 and TNF-a, were also measured. Administering alpha-pinene with APA can prevent liver damage induced by APA. Alpha-pinene can enhance TAC while reducing TOS, ALT, AST, ALP, and total bilirubin. Moreover, the results have also revealed that alpha-pinene decreases NF-kB expression, which leads to a reduction in IL-6 and TNF-a levels. It appears that alpha-pinene induces liver protective effects against APA damage by reducing the activity of liver enzymes, improving antioxidant/oxidative status, and reducing inflammation through the regulation of NF-kB and pro-inflammatory cytokines.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70227"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11793005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Positive pressure ventilation improves oxygen saturation at altitude during recreational aviation: A pilot study.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70210

Jenna L Taylor, Aidan K Downs, Crystal L Danner, J Hunter Downs, Josh Donkor, Jessica I Johnston, Douglas Rozendaal, Peter L Larsen, Bruce D Johnson, Douglas T Summerfield

{"title":"Positive pressure ventilation improves oxygen saturation at altitude during recreational aviation: A pilot study.","authors":"Jenna L Taylor, Aidan K Downs, Crystal L Danner, J Hunter Downs, Josh Donkor, Jessica I Johnston, Douglas Rozendaal, Peter L Larsen, Bruce D Johnson, Douglas T Summerfield","doi":"10.14814/phy2.70210","DOIUrl":"10.14814/phy2.70210","url":null,"abstract":"Aim: We investigated whether a commercial bi-level positive airway pressure (BPAP) device, would improve peripheral oxygen saturation (SpO2) during recreational aviation up to 12,500 feet without supplemental oxygen. Ten adults with recreational flight experience (age:47 ± 14; female = 5) completed a standardized flight profile in an unpressurised aircraft, involving randomized crossover design at 8,000 feet and 12,500 feet with BPAP or control. SpO2, middle cerebral artery velocity (MCAv), heart rate (HR), respiratory rate (RR), and relative tidal volume (TV) index were measured continuously. Psychomotor vigilance test (3-min) assessed reaction time halfway through taxi and altitude stages. Altitude significantly (p < 0.05) decreased mean SpO2, MCAv, and RR, and increased TV index and HR. There was no effect of altitude (p > 0.05) on reaction time. BPAP increased mean SpO2 at 8,000 feet [Control: 92 ± 1%; BPAP: 94 ± 2%; mean difference (MD) = 2 ± 2%; p = 0.002] and 12,500 feet [Control: 86 ± 4%; BPAP: 89 ± 4%; MD = 2 ± 3%; p = 0.013]. BPAP lowered MCAv at 8,000 feet [Control: 53 ± 10 cm/s; BPAP: 50 ± 9 cm/s; MD = -3 ± 2 cm/s; p = 0.001] and 12,500 feet [Control: 52 ± 10 cm/s; BPAP: 50 ± 8 cm/s; MD = -2 ± 3 cm/s; p = 0.041]. BPAP increased TV index at 8,000 feet (Control: 6.6 ± 1.3; BPAP:8.1 ± 1.8; MD = 1.9 ± 0.8; p < 0.001) but not 12,500 feet, without effect on RR or reaction time. This study provides preliminary results that BPAP may improve mean SpO2 for recreational aviators up to 12,500 feet without supplemental oxygen.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70210"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11794239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eight weeks of resistance exercise improves mood state and intestinal permeability in healthy adults: A randomized controlled trial.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70219

Emily Dow, Mario I Hernandez, Carol S Johnston

{"title":"Eight weeks of resistance exercise improves mood state and intestinal permeability in healthy adults: A randomized controlled trial.","authors":"Emily Dow, Mario I Hernandez, Carol S Johnston","doi":"10.14814/phy2.70219","DOIUrl":"10.14814/phy2.70219","url":null,"abstract":"To explore a potential link between resistance exercise and the gut-brain axis, this study examined the impact of resistance exercise on intestinal permeability, as indicated by lipopolysaccharide binding protein (LBP), and mood state in healthy adults. Sedentary participants (n = 20; 39.5 ± 12.1 y; 27.4 ± 5.3 kg/m2) were randomly assigned to the resistance exercise (REX) or wait-listed control (CON) groups. REX participants strength trained 3× weekly (advancing from 45%-55% to 70%-80% 1RM for 3-4 sets over 8 weeks). Strength testing, evaluation of mood states, and collection of fasting blood occurred at baseline and weeks 4 and 8. At baseline, LBP concentrations were inversely correlated to all strength measures (r range: -0.48 to -0.57; p < 0.05). The gain in total strength [(split squat left + right)/2 + bench press] was 45% higher for REX versus CON participants (p = 0.019), and serum LBP concentrations fell 16% for REX participants and rose 9% in CON participants (p = 0.014). Mood was significantly improved by resistance training versus control (but this improvement was not related to changes in LBP; r = -0.001). These findings support a role for resistance exercise in improving mood state and intestinal barrier function, but more research is warranted to further explore the effects of resistance training on the gut-brain axis.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70219"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11807843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and function of Connexin 43 and Connexin 37 in the murine zona glomerulosa.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70215

Gabriel Stölting, Nicole Hellmig, Hoang An Dinh, Frederike Butz, Ali Kerim Secener, Marina Volkert, Ute I Scholl

{"title":"Expression and function of Connexin 43 and Connexin 37 in the murine zona glomerulosa.","authors":"Gabriel Stölting, Nicole Hellmig, Hoang An Dinh, Frederike Butz, Ali Kerim Secener, Marina Volkert, Ute I Scholl","doi":"10.14814/phy2.70215","DOIUrl":"10.14814/phy2.70215","url":null,"abstract":"The zona glomerulosa (ZG) synthesizes the mineralocorticoid aldosterone. The primary role of aldosterone is the maintenance of volume and electrolyte homeostasis. Aldosterone synthesis is primarily regulated via tightly controlled oscillations in intracellular calcium levels in response to stimulation. It has previously been shown that calcium oscillations are synchronized through mechanical linkage between adjacent ZG cells. In many other cell types, similar synchronization is rather dependent on gap junctions (GJ). The recent discovery of mutations in CADM1 was linked to impaired GJ function in the ZG. Based on published transcriptomics data, we re-examined the presence and functional impact of GJ in the ZG. We found evidence for the expression of murine connexin 43 and 37 using microarray data, in-situ hybridization and immunohistology. Connexin 43 was also present in human samples. Calcium oscillations in ZG rosettes showed some degree of synchronization as reported previously. Unspecific GJ inhibition only had a small impact on this synchronicity. However, no signs of connections between cytosols could be observed as indicated by the lack of fluorescence recovery after photobleaching. We conclude that, while connexin proteins are expressed in the ZG, functional GJ in the physiological ZG are rare and of little consequence for calcium signaling.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70215"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11775451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative effects of avocado oil on bleomycin-induced lung fibrosis and oxidative stress in rats.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70228

Naoures Ochi, Anouar Abidi, Wael Taamalli, Ayda Ayedi, Hichem Sebai

{"title":"Ameliorative effects of avocado oil on bleomycin-induced lung fibrosis and oxidative stress in rats.","authors":"Naoures Ochi, Anouar Abidi, Wael Taamalli, Ayda Ayedi, Hichem Sebai","doi":"10.14814/phy2.70228","DOIUrl":"10.14814/phy2.70228","url":null,"abstract":"Pulmonary fibrosis (PF), is a chronic interstitial lung disease, characterized by changes in the alveoli, excessive accumulation of extracellular matrix, persistent inflammation, and oxidative stress. In this study, we aimed to explore the therapeutic effects of avocado oil (Ao) on bleomycin (BLM)-induced PF. Four this, 24 male rats were divided into four groups (n = 6): the first group served as a control, the second served as a fibrotic group, instilled intratracheally only with BLM (2 mg/kg bw), and the remaining groups were treated by gastric gavage with Ao at different doses (3.5 and 5 mL/kg bw) for 25 days after BLM instillation. The fibrosis induction revealed significant alterations, including increased lipid peroxidation and decreased antioxidant enzyme activities such as superoxyde dismutase (SOD) and catalase (CAT), level of thiols group coupled with a high fibrosis score (FS) and an inflammatory index (II), along with excessive collagen deposition in the pulmonary interstitium. Ao treatment reversed all disturbances induced by BLM in oxidative stress parameters and relatively repairs the histological damage caused by BLM by reducing the FS and the II. The antioxidant, anti-inflammatory and anti-fibrosis power of Ao, may suggest this last as a promising candidate for the treatment of PF.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70228"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prolonged sitting is not associated with altered shear-mediated dilation of the internal carotid artery, despite impairing lower limb endothelial function.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70097

Shotaro Saito, Kento Dora, Marino Karaki, Narumi Kunimatsu, Hayato Tsukamoto, Jun Sugawara, Erika Iwamoto, Shigehiko Ogoh

{"title":"Prolonged sitting is not associated with altered shear-mediated dilation of the internal carotid artery, despite impairing lower limb endothelial function.","authors":"Shotaro Saito, Kento Dora, Marino Karaki, Narumi Kunimatsu, Hayato Tsukamoto, Jun Sugawara, Erika Iwamoto, Shigehiko Ogoh","doi":"10.14814/phy2.70097","DOIUrl":"10.14814/phy2.70097","url":null,"abstract":"The present study aims to examine the effect of 4 h of continuous sitting on cerebral endothelial function, which is a crucial component of cerebral blood flow regulation. We hypothesized that 4 h of sitting may impair cerebral endothelial function similarly to how it affects lower limb vasculature. Thirteen young, healthy participants were instructed to remain seated for 4 h without moving their lower limbs. The blood flow and shear rate (SR) in the popliteal and internal carotid artery (ICA) were measured using duplex Doppler ultrasound. During the 4-h sitting, peripheral (popliteal artery) and cerebral (ICA) endothelial function were assessed every hour. We induced peripheral and cerebral flow-mediated dilation (pFMD and ICA FMD) using hyperemia (5 min of cuff inflation on lower limb, then deflation) or hypercapnia (30s of hypercapnia, end-tidal partial pressure of CO2 + 9 mmHg), respectively. We then calculated each relative peak dilation from the baseline diameter to identify both pFMD and ICA FMD. We observed a significant decrease in pFMD starting at 2 h from the onset of sitting, and this reduction persisted throughout the 4-h sitting [Base (6.8 ± 4.2%) vs. 2-h (3.9 ± 2.0%), p = 0.044; vs. 3-h (3.2 ± 1.8%), p = 0.016; vs. 4-h (3.2 ± 1.9%), p = 0.005]. In contrast, during the 4-h sitting, ICA blood flow, SR, and ICA FMD remained unchanged (p = 0.062, p = 0.068, and p = 0.203, respectively). Unlike peripheral endothelial function, cerebral endothelial function remained stable during 4-h sitting. This suggests that the acute effect of prolonged sitting on cerebral vasculature differs from that of lower limb vasculature.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70097"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11776389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporary bilateral clamping of renal arteries induces ischemia-reperfusion: A new pig model of acute kidney injury using total intravenous anesthesia.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70203

Axel Guilpin, Mathieu Magnin, Axel Aigle, Jean-Yves Ayoub, Timothée Schuhler, Romain Lac, Thierry Marchal, Thomas Brichart, Abdessalem Hammed, Vanessa Louzier

{"title":"Temporary bilateral clamping of renal arteries induces ischemia-reperfusion: A new pig model of acute kidney injury using total intravenous anesthesia.","authors":"Axel Guilpin, Mathieu Magnin, Axel Aigle, Jean-Yves Ayoub, Timothée Schuhler, Romain Lac, Thierry Marchal, Thomas Brichart, Abdessalem Hammed, Vanessa Louzier","doi":"10.14814/phy2.70203","DOIUrl":"10.14814/phy2.70203","url":null,"abstract":"Ischemia-reperfusion (IR) is a leading cause of acute kidney injury (AKI), and pigs are commonly used in preclinical AKI models. However, existing models often vary in the methods used to induce ischemia, and the resulting AKI tends to be mild-to-moderate. Moreover, follow-up is often performed under volatile anesthesia, which, in contrast to total intravenous anesthesia (TIVA), can induce malignant hyperthermia and cause hemodynamic instability. Here we present a novel surgical model of IR-induced AKI using bilateral renal artery clamping under TIVA. Anesthesia was induced via TIVA with diazepam, ketamine, and morphine. After retroperitoneal exposure, the renal arteries were isolated and clamped with a plastic tube for 90 min, followed by 8 h of reperfusion. The IR group (n = 6) was compared with a Sham group (n = 5) that underwent the same procedure without IR. The IR group developed moderate-to-severe AKI as evidenced by reduced glomerular filtration, a 158% increase in plasma creatinine versus 21% in the Sham group, and elevated neutrophil gelatinase-associated lipocalin levels (+280% in IR vs. 0% in Sham), indicating tubular injury. Histopathology confirmed these findings. Thus, this preclinical model successfully induced moderate-to-severe AKI in pigs. The TIVA anesthetic protocol offered several advantages compared to halogenated gas anesthesia.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70203"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11788332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing a meditation module to affect etiological and preventive factors in primary hypertensive patients-A pilot study.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70226

Kapila Goswami Sharma, S D Manjula, Shobha U Kamath, M Mukhyaprana Prabhu, M G Ramesh Babu, Ujjal Bose, K Vasanthalaxmi

{"title":"Designing a meditation module to affect etiological and preventive factors in primary hypertensive patients-A pilot study.","authors":"Kapila Goswami Sharma, S D Manjula, Shobha U Kamath, M Mukhyaprana Prabhu, M G Ramesh Babu, Ujjal Bose, K Vasanthalaxmi","doi":"10.14814/phy2.70226","DOIUrl":"10.14814/phy2.70226","url":null,"abstract":"The aim of the present pilot study is to design, validate, and check the feasibility and efficacy of the designed meditation module on etiological and preventive factors in primary hypertensive patients (PHP). The systematic and detailed meditation module was formulated to prevent the complications of hypertension. The designed module was validated by 5 subject experts in the field of complementary and alternative therapy and research. Content Validity Ratio (CVR), Average Congruency Percentage (ACP), and Content Validity Index were calculated. The intervention was given for 5 days a week for 4 weeks, to 9 PHP to check the feasibility and the efficacy of the module by analyzing biochemical and psychological parameters. A randomized controlled trial is planned ahead with this module as intervention. The designed meditation module had 19 concepts with CVR >0.49 and ACP > 90% which were retained, 2 were modified (CVR 0.6, ACP 80) and 1 was deleted as CVR was <0.49 and ACP < 70%. The feasibility test mean was 93.33 ± 8.66 and the efficacy was tested by increase in the brain-derived neurotrophic factor (BDNF) (p = 0.022) and decrease in anxiety (p = 0.022) and perceived stress (p = 0.016), improvement in emotional quotient (p = 0.011), mental state (p = 0.044), quality of sleep (p = 0.001), quality of life (p = 0.011), and happiness state (p = 0.003). The designed meditation module was found valid by the experts, feasible to patients, and efficient in improving biochemical and psychological parameters among PHP to affect the hypertensive etiological factors and check the progress and complications of the disorder. Trial Registration: CTRI/2022/09/045421.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70226"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11802630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RBT-1, a "preconditioning" agent, mitigates syndecan-1 shedding in patients undergoing "on pump" cardiac surgery and following experimental AKI.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70218

Ali C M Johnson, Richard A Zager

{"title":"RBT-1, a \"preconditioning\" agent, mitigates syndecan-1 shedding in patients undergoing \"on pump\" cardiac surgery and following experimental AKI.","authors":"Ali C M Johnson, Richard A Zager","doi":"10.14814/phy2.70218","DOIUrl":"10.14814/phy2.70218","url":null,"abstract":"During systemic stress, syndecan-1 (SDC-1) shedding into plasma results, implying endothelial damage. RBT-1, a \"preconditioning\" agent, has been shown to mitigate postoperative complications following cardiac surgeries. This study assessed whether RBT-1 preconditioning attenuated SDC-1 shedding in these patients, implying a vascular protective effect. Patients (n, 112) were randomized to receive low-dose RBT-1, high-dose RBT-1, or placebo 24-48 h prior to surgery. Plasma samples were obtained before and 2 days postsurgery and assayed for SDC-1 (ELISA). To gain further insights, male CD-1 mice were subjected to acute renal injuries, and RBT-1's impact on plasma SDC-1 increases, vascular/aortic stress responses (NGAL/KIM-1/IL-6 gene induction), and two vascular cytoprotective pathways (Nrf2; ferritin) were assessed. Baseline plasma SDC-1 levels did not differ between patient groups. The placebo group developed an approximate 50% plasma SDC-1 (ng/mL) increase (p, 0.012). Conversely, no significant SDC-1 increases were seen in the RBT-1 treatment groups. Experimental injury markedly increased plasma SDC-1 concentrations, and these were significantly blunted by RBT-1 preconditioning. RBT-1 also mitigated AKI-induced aortic NGAL/KIM-1/IL-6 mRNA increases, activated aortic Nrf2, and increased vascular ferritin levels. RBT-1 preconditioning diminishes SDC-1 release and upregulates vascular ferritin and Nrf2. Hence, RBT-1 preconditioning can confer select vasoprotective effects.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70218"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11794240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postnatal enteral plasma supplementation following birth asphyxia increases fluid retention and kidney health in newborn pigs.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70238

Jingren Zhong, Stanislava Pankratova, Richard Doughty, Christoffer Kirkelund Flyger, Per Torp Sangild, Kerstin Skovgaard, Henrik Elvang Jensen, Duc Ninh Nguyen, Thomas Thymann

{"title":"Postnatal enteral plasma supplementation following birth asphyxia increases fluid retention and kidney health in newborn pigs.","authors":"Jingren Zhong, Stanislava Pankratova, Richard Doughty, Christoffer Kirkelund Flyger, Per Torp Sangild, Kerstin Skovgaard, Henrik Elvang Jensen, Duc Ninh Nguyen, Thomas Thymann","doi":"10.14814/phy2.70238","DOIUrl":"10.14814/phy2.70238","url":null,"abstract":"Birth asphyxia can result in kidney dysfunction, disturbances in systemic electrolytes and fluid balance in newborns. Currently, there is no proven dietary approach to support asphyxiated newborns. This study investigates whether oral plasma supplementation improves kidney function and overall health in asphyxiated newborns. Cesarean-delivered near-term pigs with or without an 8 min intrauterine clamping of the umbilical cord were fed a milk replacer dissolved in water for 24 h in Experiment 1. Pigs were fed 72 h with milk replacers dissolved in either maternal plasma or water in Experiment 2. Blood, urine, and kidney tissue were collected for further analyses. Asphyxia disrupted blood electrolyte balance. And plasma feeding led to higher fluid retention for both asphyxiated and control pigs. Additionally, plasma feeding may also affect kidney development and protect kidneys from asphyxia induced impairments. Birth asphyxia in pigs led to immediate disturbance of electrolyte balance, impaired fluid retention, and kidney impairments. Plasma feeding may improve postnatal newborn hydration and may also improve the condition of kidneys following asphyxia.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70238"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11798866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0