Physiological Reports最新文献_第7页

Improved glucose handling in female rat offspring of a hypertensive pregnancy with intrauterine growth restriction.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70222

Melissa A Cedars, Kate M Root, Brian Akhaphong, Megan Beetch, Abigail E Miles, Ronald R Regal, Emilyn U Alejandro, Jean F Regal

{"title":"Improved glucose handling in female rat offspring of a hypertensive pregnancy with intrauterine growth restriction.","authors":"Melissa A Cedars, Kate M Root, Brian Akhaphong, Megan Beetch, Abigail E Miles, Ronald R Regal, Emilyn U Alejandro, Jean F Regal","doi":"10.14814/phy2.70222","DOIUrl":"10.14814/phy2.70222","url":null,"abstract":"Hypertensive disorders of pregnancy, intrauterine growth restriction (IUGR), and reduced pancreatic β-cell area increases risk of offspring developing type 2 diabetes (T2D). Our previous studies using rat reduced uteroplacental perfusion pressure (RUPP) model of gestational hypertension and IUGR demonstrated reduced pancreatic β-cell area in offspring at embryonic day 19 and postnatal day 13 (PD13). We hypothesized reduced β-cell area early in life would manifest as hyperglycemia and glucose intolerance as animals aged. However, glucose intolerance did not differ in RUPP versus control offspring to 1 year of life, whether intraperitoneal or oral glucose challenge. At PD28, female RUPP offspring show normalized β-cell area compared to controls and improved ability to clear glucose following oral challenge. Oral glucose challenge results in significant increase in incretin GLP-1 in RUPP female offspring compared to control. Insulin tolerance did not differ amongst control and RUPP offspring, except at PD28 where insulin reduced blood glucose more effectively in RUPP female offspring versus control. Insulin-induced vasodilation in isolated aorta and insulin signaling in fat are more pronounced in RUPP PD28 female offspring versus control. Thus, our studies demonstrate compensatory mechanisms protect IUGR offspring of a hypertensive pregnancy from long-term metabolic effects and development of T2D.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70222"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the role of beta-endorphin in activity-based anorexia in mice.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70201

Connor W Christensen, Samantha E Weed, Travis E Brown, Shane T Hentges

{"title":"Exploring the role of beta-endorphin in activity-based anorexia in mice.","authors":"Connor W Christensen, Samantha E Weed, Travis E Brown, Shane T Hentges","doi":"10.14814/phy2.70201","DOIUrl":"10.14814/phy2.70201","url":null,"abstract":"Anorexia nervosa (AN) remains one of the most lethal mental health disorders and is poorly understood from a neurobiological perspective. The most widely used animal model of AN is activity-based anorexia (ABA) where scheduled food presentation leads to a spontaneous maladaptive increase in running-wheel activity and rapid weight loss in rodents, recapitulating specific aspects of AN. Research using the ABA paradigm to probe the role of hedonic and homeostatic circuits has indicated that the hypothalamic proopiomelanocortin (POMC) system may play a role in both the increased activity and reduced food intake observed. Previous work has shown that Pomc mRNA and its peptide product beta-endorphin (β-end) are increased during the onset of ABA. β-end is reinforcing and increases locomotor activity, and mice lacking the mu opioid receptor (MOR), the primary target of β-end, display blunted food-anticipatory activity in the ABA paradigm. Thus, the current work was designed to determine if aspects of ABA would be diminished in mice lacking β-end. We did not find any significant differences in wheel-running, food intake, or body weight loss in β-end knockout mice of either sex during ABA compared to wild-type littermates. Therefore, we conclude that the development of ABA does not require β-end.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70201"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11810985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A method for MRI-guided bronchoscopy to identify obstructed airway segments.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70119

David G Mummy, Marrissa J McIntosh, Katherine J Carey, Shannon Kehoe, Stephane Esnault, Mats W Johansson, Michael D Evans, Ronald L Sorkness, Mark Schiebler, Nizar N Jarjour, Loren C Denlinger, Sean B Fain

{"title":"A method for MRI-guided bronchoscopy to identify obstructed airway segments.","authors":"David G Mummy, Marrissa J McIntosh, Katherine J Carey, Shannon Kehoe, Stephane Esnault, Mats W Johansson, Michael D Evans, Ronald L Sorkness, Mark Schiebler, Nizar N Jarjour, Loren C Denlinger, Sean B Fain","doi":"10.14814/phy2.70119","DOIUrl":"10.14814/phy2.70119","url":null,"abstract":"Bronchoscopy is not conventionally guided by prior knowledge of segmental airway obstruction. Hyperpolarized gas magnetic resonance imaging (MRI) ventilation abnormalities and computed tomography (CT) air trapping are related to lung function and asthma severity but have not been used to target segmental inflammation and remodeling. We evaluate the feasibility of using bronchoscopy guided by 3He MRI and CT to reveal differences in inflammatory response, morphology, and cellular activity in poorly- (defect) versus well-ventilated (control) lung regions. Eleven participants (5 female; age, 22.8 ± 3.4 years; 9 asthma) who experienced a cold with increased lower airway symptoms underwent 3He MRI and/or CT at least 6 weeks after recovery. Differences between defect and control regions were compared. In defect as compared to control sites, bronchoalveolar lavage neutrophils (p = 0.06) and granulocytes (p = 0.08) trended towards an increase; inflammatory mediators (i.e., 15-epi-LXA4, LXA4) were also significantly different (p < 0.05) between sites. Correlations were observed between macrophages, neutrophils, and eosinophils with inflammatory mediators (i.e., 15-epi-LXA4, LXA4, LTB4). Correlations were observed for macrophages and neutrophils with 15-epi-LXA4, and eosinophils with LXA4 and leukotriene B4. Basement membrane wall thickness was similar for defect versus control sites (p = 0.9). These results support the feasibility of image-guided methods to identify airway obstruction phenotypes.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 4","pages":"e70119"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of training-induced alterations in body composition via automated machine learning analysis of MRI images in the thigh region: A pilot study in young females.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70187

Saied Ramedani, Ebru Kelesoglu, Norman Stutzig, Hendrik Von Tengg-Kobligk, Keivan Daneshvar Ghorbani, Tobias Siebert

{"title":"Quantification of training-induced alterations in body composition via automated machine learning analysis of MRI images in the thigh region: A pilot study in young females.","authors":"Saied Ramedani, Ebru Kelesoglu, Norman Stutzig, Hendrik Von Tengg-Kobligk, Keivan Daneshvar Ghorbani, Tobias Siebert","doi":"10.14814/phy2.70187","DOIUrl":"10.14814/phy2.70187","url":null,"abstract":"The maintenance of an appropriate ratio of body fat to muscle mass is essential for the preservation of health and performance, as excessive body fat is associated with an increased risk of various diseases. Accurate body composition assessment requires precise segmentation of structures. In this study we developed a novel automatic machine learning approach for volumetric segmentation and quantitative assessment of MRI volumes and investigated the efficacy of using a machine learning algorithm to assess muscle, subcutaneous adipose tissue (SAT), and bone volume of the thigh before and after a strength training. Eighteen healthy, young, female volunteers were randomly allocated to two groups: intervention group (IG) and control group (CG). The IG group followed an 8-week strength endurance training plan that was conducted two times per week. Before and after the training, the subjects of both groups underwent MRI scanning. The evaluation of the image data was performed by a machine learning system which is based on a 3D U-Net-based Convolutional Neural Network. The volumes of muscle, bone, and SAT were each examined using a 2 (GROUP [IG vs. CG]) × 2 (TIME [pre-intervention vs. post-intervention]) analysis of variance (ANOVA) with repeated measures for the factor TIME. The results of the ANOVA demonstrate significant TIME × GROUP interaction effects for the muscle volume (F1,16 = 12.80, p = 0.003, ηP 2 = 0.44) with an increase of 2.93% in the IG group and no change in the CG (-0.62%, p = 0.893). There were no significant changes in bone or SAT volume between the groups. This study supports the use of artificial intelligence systems to analyze MRI images as a reliable tool for monitoring training responses on body composition.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70187"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11776390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peripheral blood mononuclear cell number and paracrine function in responses to a 50-km trail race: An exploratory study.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70255

Rian Q Landers-Ramos, Katherine Kim, James Heilman, William S Evans, Odessa Addison, Sushant M Ranadive, Steven J Prior

{"title":"Peripheral blood mononuclear cell number and paracrine function in responses to a 50-km trail race: An exploratory study.","authors":"Rian Q Landers-Ramos, Katherine Kim, James Heilman, William S Evans, Odessa Addison, Sushant M Ranadive, Steven J Prior","doi":"10.14814/phy2.70255","DOIUrl":"10.14814/phy2.70255","url":null,"abstract":"Peripheral blood mononuclear cells (PBMCs) represent a heterogeneous mix of cells with paracrine functions that may be altered following prolonged exercise. We determined the effect of ultramarathon running on PBMC paracrine function and PBMC subtype number. Recreational athletes participated in a 50 km ultramarathon. Blood was sampled from N = 7 at baseline, 10 km, 50 km, and 24 h post-race. PBMCs were isolated and cultured, and conditioned media was used for a HUVEC-based proliferation assay. CD31+, CD3+, and CD31+/CD3+ PBMCs were quantified at each time point. Proliferation increased from baseline to 50 km (p = 0.004) and was reduced from 50 km to 24 h post (p = 0.008). There was an increase in CD31+ PBMCs after 50 km (p = 0.014), returning to baseline at 24 h post-race (p = 0.246). CD3+ PBMC and CD31+/CD3+ PBMC numbers were reduced after 50 km (p = 0.001 and p = 0.002, respectively), returning to baseline levels 24 h post-race (p = 0.190 and p = 0.315, respectively). PBMC paracrine activity following a 50 km enhances endothelial cell proliferation. Alterations in PBMC subtypes after 50 km suggest a protective role of PBMCs in response to prolonged stresses of ultramarathon running.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 4","pages":"e70255"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11839398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time-dependent bladder activity changes in streptozotocin-induced female diabetic rats.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70220

Keiichiro Izumi, Tadanobu Chuyo Kamijo, Takuma Oshiro, Ryu Kimura, Asuka Ashikari, Masahiro Kurobe, Takahiro Akimoto, Minoru Miyazato

{"title":"Time-dependent bladder activity changes in streptozotocin-induced female diabetic rats.","authors":"Keiichiro Izumi, Tadanobu Chuyo Kamijo, Takuma Oshiro, Ryu Kimura, Asuka Ashikari, Masahiro Kurobe, Takahiro Akimoto, Minoru Miyazato","doi":"10.14814/phy2.70220","DOIUrl":"10.14814/phy2.70220","url":null,"abstract":"This study aimed to investigate the long-term physiological and morphological changes in the bladders of diabetic rats. Sixty-nine female Sprague-Dawley rats were divided into a control and six diabetic (3 days and 2, 4, 8, 12, and 24 weeks after induction of type 1 diabetes) groups. Metabolic cages and cystometry were used to evaluate bladder function. Bladder contractility was assessed using an organ bath test, and Masson's trichrome staining was performed. In the metabolic cage study, the urination frequency during the dark period significantly increased in the early stages at 3 days of diabetes (p < 0.05). The voiding interval significantly increased (p < 0.05) at 8-12 weeks of diabetes, while the residual urine volume and voiding efficiency worsened at 24 weeks. In the organ bath study, the dose-response curve of carbachol for median effective concentration did not change; however, the bladder contractile force was enhanced at 8 weeks (p = 0.028). Histological analysis revealed increased fibrosis at 4 weeks of diabetes. Diabetic bladder dysfunction is characterized by storage and voiding bladder activity changes in the early stages that induces urinary frequency and reduced voiding efficiency in the late phase; this turning point occurs at 8 weeks after diabetes.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 4","pages":"e70220"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Age-related differences in agility are related to both muscle strength and corticospinal tract function.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70223

Evan G MacKenzie, Nick W Bray, Syed Z Raza, Caitlin J Newell, Hannah M Murphy, Michelle Ploughman

{"title":"Age-related differences in agility are related to both muscle strength and corticospinal tract function.","authors":"Evan G MacKenzie, Nick W Bray, Syed Z Raza, Caitlin J Newell, Hannah M Murphy, Michelle Ploughman","doi":"10.14814/phy2.70223","DOIUrl":"10.14814/phy2.70223","url":null,"abstract":"Agility is essential for \"healthy\" aging, but neuromuscular contributions to age-related differences in agility are not entirely understood. We recruited healthy (n = 32) non-athletes (30-84 years) to determine: (1) if aging is associated with agility and (2) whether muscle strength or corticospinal tract function predicts agility. We assessed muscle strength via a validated knee extension test, corticospinal tract function via transcranial magnetic stimulation, and agility via spatiotemporal values (i.e., leg length-adjusted hop length and hop length variability) collected during a novel propulsive bipedal hopping (agility) task on an electronic walkway. Pearson correlation revealed aging is associated with leg length-adjusted hop length (r = -0.671, p < 0.001) and hop length variability (r = 0.423, p = 0.016). Further, leg length-adjusted hop length and hop length variability correlated with quadriceps strength (r = 0.581, p < 0.001; r = -0.364, p = 0.048) and corticospinal tract function (r = -0.384, p = 0.039; r = 0.478, p = 0.007). However, hierarchical regressions indicated that, when controlling for sex, muscle strength only predicts leg length-adjusted hop length (R2 = 0.345, p = 0.002), whereas corticospinal tract function only predicts hop length variability (R2 = 0.239, p = 0.014). Therefore, weaker quadriceps decrease the distance hopped, and deteriorating corticospinal tract function increases variability in hop length.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 4","pages":"e70223"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11845323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regional cerebral pulsatile hemodynamics during isocapnic and poikilocapnic hyperthermia in young men.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70258

Spencer J Skaper, Brooke R Shepley, Ibrahim Amr Wafai, Philip N Ainslie, Anthony R Bain, Kurt J Smith

{"title":"Regional cerebral pulsatile hemodynamics during isocapnic and poikilocapnic hyperthermia in young men.","authors":"Spencer J Skaper, Brooke R Shepley, Ibrahim Amr Wafai, Philip N Ainslie, Anthony R Bain, Kurt J Smith","doi":"10.14814/phy2.70258","DOIUrl":"10.14814/phy2.70258","url":null,"abstract":"Hyperthermia is known to induce hypocapnia-driven reductions in cerebral blood flow; however, it is unknown if it causes changes in hemodynamic pulsatility that negatively influence cerebrovascular function. This retrospective analysis aimed to assess cerebrovascular hemodynamic pulsatile buffering (damping factor; DFi) during poikilocapnic (HT) and isocapnic (HT-C) hyperthermia. We hypothesized that HT would reduce cerebral DFi, while HT-C would attenuate the reduction in DFi by limiting increases in resistance. Ten healthy males were passively heated +2°C from normothermia (BL). Blood flow through the internal carotid artery (ICA) and vertebral artery (VA) was measured using vascular ultrasound. Blood velocity through the middle cerebral artery (MCA) and the posterior cerebral artery (PCA) was measured using transcranial ultrasound. DFi was calculated as the ratio of proximal to distal pulsatility index (PI): Anterior cerebral DFi = PIICA/PIMCA; Posterior cerebral DFi = PIVA/PIPCA. Anterior DFi decreased in both HT (1.08 ± 0.19 a.u; p = 0.007) and HT-C (1.12 ± 0.231 a.u; p = 0.021) conditions from BL values (1.27 ± 0.14 a.u). No changes were observed in posterior DFi, p = 0.116. Irrespective of PaCO2 clamping, both hyperthermic conditions reduced anterior DFi, suggesting other mechanisms are responsible for cerebrovascular hemodynamic buffering. Posterior DFi responses were not observed, suggesting preferential buffering of the hyperthermic posterior circulation (VA-PCA).","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 4","pages":"e70258"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11847895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organ development in growth-restricted fetuses in the reduced uterine perfusion pressure rat model: A vascular approach of brain, heart, and kidney.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70244

J Alhama-Riba, C M van Kammen, K T Nijholt, D Viveen, K Amarouchi, D Shasha, M M Krebber, F E Hoebeek, A T Lely, C H A Nijboer, F Terstappen

{"title":"Organ development in growth-restricted fetuses in the reduced uterine perfusion pressure rat model: A vascular approach of brain, heart, and kidney.","authors":"J Alhama-Riba, C M van Kammen, K T Nijholt, D Viveen, K Amarouchi, D Shasha, M M Krebber, F E Hoebeek, A T Lely, C H A Nijboer, F Terstappen","doi":"10.14814/phy2.70244","DOIUrl":"10.14814/phy2.70244","url":null,"abstract":"Fetal growth restriction (FGR) increases the risk of developing cardiovascular, renal, and neurovascular diseases. An overlapping vascular pathophysiology as a response to chronic hypoxia and circulatory redistribution in utero, might underlie this lifelong burden. This study aims to assess potential vascular detoriations in multiple organs following FGR using the Reduced Uterine Perfusion Pressure (RUPP) rat model. The fetal brain, heart, and kidney were collected (RUPP n = 16 vs. sham n = 13) at embryonic day (E)19 for histological assessment of various aspects of vascular and structural development. Results indicated similar microvascularisation in all organs between the groups. Structural assessment demonstrated a decreased brain area and thickness of the somatosensory cortex and thicker right ventricular wall of the heart (not driven by increased proliferation) in RUPP fetuses, and no differences in renal development. In conclusion, the fetal stage might be too early to detect detoriation in organ vasculature, while this study did reveal subtle alterations in structural development of mostly the brain, followed by the heart with sparing of the kidneys. Potentially compensatory mechanisms may be at play at this fetal stage. Nevertheless, small subclinical adaptations could make the FGR offspring more susceptible for second hits with manifestation at older age.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70244"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11821727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143409871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

L-arginine ameliorates hypertension and cardiac mitochondrial abnormalities but not cardiac injury in male metabolic syndrome rats.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70183

Kaito Tagami, Touko Okuzawa, Keisuke Yoshida, Rin Mishima, Natsuki Obara, Asuko Kunimatsu, Mayako Koide, Tamami Teranishi, Koji Itakura, Katsuhide Ikeda, Toyoaki Murohara, Kohzo Nagata

{"title":"L-arginine ameliorates hypertension and cardiac mitochondrial abnormalities but not cardiac injury in male metabolic syndrome rats.","authors":"Kaito Tagami, Touko Okuzawa, Keisuke Yoshida, Rin Mishima, Natsuki Obara, Asuko Kunimatsu, Mayako Koide, Tamami Teranishi, Koji Itakura, Katsuhide Ikeda, Toyoaki Murohara, Kohzo Nagata","doi":"10.14814/phy2.70183","DOIUrl":"10.14814/phy2.70183","url":null,"abstract":"L-Arginine supplementation has beneficial effects on metabolic disorders in rodents. We here investigated the effects of exogenous L-arginine on cardiac pathology and mitochondrial reactive oxygen species (ROS) production and dynamics in DahlS.Z-Leprfa/Leprfa (DS/obese) rats, a model of metabolic syndrome (MetS). DS/obese rats and their lean homozygous littermate (DahlS.Z-Lepr+/Lepr+, or DS/lean) controls were provided with drinking water containing 0.50% L-arginine-HCl or 0.85% L-alanine (isonitrogenous control) from 13 to 17 weeks of age. L-Arginine supplementation markedly alleviated hypertension without affecting cardiac injury in MetS rats. It also attenuated the increase in ROS production apparent in cardiac mitochondria isolated from MetS rats as well as suppressed the associated upregulation of Nox4 mRNA and protein in the heart. Furthermore, L-arginine reversed the decrease in the size of cardiac mitochondria as well as changes in the expression of DRP1 and OPA1 proteins apparent in the L-alanine-treated MetS rat heart. Cardiac arginase II gene expression and arginase activity were increased by L-arginine treatment in MetS rats but not CONT rats. L-Arginine supplementation thus ameliorated hypertension and cardiac mitochondrial abnormalities in MetS rats, with the lack of a cardioprotective effect possibly being due to increased arginase activity.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 4","pages":"e70183"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0