Physiological Reports最新文献_第7页

Vascular remodeling in arteriovenous fistula treated with PDE5A inhibitors. PDE5A抑制剂治疗动静脉瘘血管重构。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70331

Maheshika Somarathna, Hannah Northrup, Kevin Ingle, Tatyana Isayeva-Waldrop, Nguyen Thuy Nhu Nguyen, Bailey Lose, Yan-Ting Shiu, Timmy Lee

{"title":"Vascular remodeling in arteriovenous fistula treated with PDE5A inhibitors.","authors":"Maheshika Somarathna, Hannah Northrup, Kevin Ingle, Tatyana Isayeva-Waldrop, Nguyen Thuy Nhu Nguyen, Bailey Lose, Yan-Ting Shiu, Timmy Lee","doi":"10.14814/phy2.70331","DOIUrl":"https://doi.org/10.14814/phy2.70331","url":null,"abstract":"The arteriovenous fistula (AVF) is the lifeline for hemodialysis patients. However, there are currently no effective therapies promoting AVF maturation. AVF dilation by smooth muscle cell relaxation, through increased cyclic guanosine monophosphate (cGMP), is one potential mechanism to improve AVF remodeling. In this study, we examined the cGMP pathway and its inhibitor phosphodiesterase 5A (PDE5A) in rat, pig, and human AVF. We administered the PDE5A inhibitor, sildenafil, to rats with femoral AVFs and analyzed AVF histological and hemodynamic parameters. We observed that AVF creation increases PDE5A expression in rodent and porcine AVF models. Similarly, we observed an increase in PDE5A expression in the anastomotic regions of AVFs from hemodialysis patients when compared to pre-AVF placement. Sildenafil-treated rats showed significantly increased ultrasound-derived AVF volumetric blood flow and increased MRI-derived 3-dimensional lumen diameter when compared to controls. MRI-based computational fluid dynamics showed that sildenafil-treated rats had increased anastomotic hemodynamics compared to control rats. Histology showed similar intimal hyperplasia in sildenafil-treated and control rats. In conclusion, sildenafil treatment increases AVF vein outward expansion and blood flow without affecting the level of intimal hyperplasia. PDE5A inhibitors serve as a potential therapeutic approach to promote AVF maturation by enhancing outward vascular remodeling.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70331"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12040441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ASIC currents in cultured primate retinal amacrine/ganglion cells. 培养灵长类视网膜无突/神经节细胞的ASIC电流。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70290

Talib Saafir, Tiandong Leng, Koichi Inoue, Zhi-Gang Xiong, Peter MacLeish

{"title":"ASIC currents in cultured primate retinal amacrine/ganglion cells.","authors":"Talib Saafir, Tiandong Leng, Koichi Inoue, Zhi-Gang Xiong, Peter MacLeish","doi":"10.14814/phy2.70290","DOIUrl":"10.14814/phy2.70290","url":null,"abstract":"Acid-sensing ion channels (ASICs) are proton-gated cation channels belonging to the epithelial Na + channel/degenerin superfamily. In the CNS, ASICs are involved in synaptic plasticity, learning/memory, and acidosis-mediated injury. Previous studies showed that ASICs are expressed in rodent retina where activation likely participates in the phototransduction process and retinal integrity. However, there have been no studies examining the expression of ASICs in primate retina. Using molecular biology and patch-clamp techniques, we explored the expression of ASICs in monkey retina and cultured monkey retinal cells, and the electrophysiological/pharmacological properties of ASICs in cultured amacrine/ganglion cells. RT-PCR detected the expression of ASIC1a, 2a, 3, and 4 in intact monkey retina and cultured retinal cells. Patch-clamp recordings showed transient ASIC currents with a pH 0.5 of 4.69. The currents were almost completely blocked by amiloride (100 μM) but were insensitive to PcTx-1 (20 nM). The currents were potentiated by zinc (100 μM) and showed recovery from desensitization with a time constant of 0.18 s and were resistant to low conditioning pH with a pH 0.5 for steady-state inactivation of 6.45. Our results for the first time demonstrate the expression of functional ASICs in primate amacrine/ganglion cells and suggest that ASIC currents in these cells are mediated predominantly by ASIC2a containing channels.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70290"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12069859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of interval versus continuous exercise on cerebral vascular flow-mediated dilatation in young healthy males. 间歇运动与连续运动对年轻健康男性脑血管血流介导的扩张的影响。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70354

Harvey J Walsh, Shotaro Saito, Narumi Kunimatsu, Marino Karaki, James P Fisher, Shigehiko Ogoh

{"title":"Effects of interval versus continuous exercise on cerebral vascular flow-mediated dilatation in young healthy males.","authors":"Harvey J Walsh, Shotaro Saito, Narumi Kunimatsu, Marino Karaki, James P Fisher, Shigehiko Ogoh","doi":"10.14814/phy2.70354","DOIUrl":"https://doi.org/10.14814/phy2.70354","url":null,"abstract":"Aerobic exercise reduces the risk of cerebrovascular dysfunction. One proposed mechanism is exercise-induced increases in cerebral shear stress (SS) improving cerebral endothelial function. A recent report indicated that interval exercise (Int-Ex) induces greater cerebral SS than continuous exercise (Con-Ex); however, its effect on cerebral endothelial function remains unclear. We hypothesized that Int-Ex would enhance cerebral SS and, consequently, cerebral endothelial function more than Con-Ex. Fourteen healthy males (21 ± 0.6 years) completed 32 min of Int-Ex and work-equivalent Con-Ex on a semi-recumbent bike on separate days. Cerebrovascular flow-mediated dilatation (cFMD) was assessed before exercise (Pre), 15 min (Post-15) and 40 min post-exercise (Post-40). cFMD was defined as peak internal carotid artery vasodilatation (Δ% from baseline; Duplex ultrasound) in response to a 30-s hypercapnic exposure, raising end-tidal partial pressure of carbon dioxide by ~7 mmHg. Post-exercise cerebral SS was greater after Int-Ex versus Con-Ex (p = 0.002). Int-Ex evoked a ~37% increase in post-exercise cerebral SS compared to rest, with a negligible increase for Con-Ex. cFMD did not differ between Int-Ex and Con-Ex trials before exercise (Pre, 6.35 ± 3.89% vs. 5.54 ± 3.83%; p = 0.542) and remained unchanged post-exercise (Post-15, 7.20 ± 4.47% vs. 6.13 ± 4.08%; Post-40, 5.69 ± 3.86% vs. 6.94 ± 3.55%; p = 0.583). These results indicate that Int-Ex and Con-Ex have similar acute effects on cerebral endothelial function.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70354"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12051378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid exposure leads to metabolic dysfunction in fetal sheep cardiomyocytes. 脂质暴露导致胎羊心肌细胞代谢功能障碍。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70386

Natasha N Chattergoon, Karthikeyan Bose, Samantha Louey, Sonnet S Jonker

{"title":"Lipid exposure leads to metabolic dysfunction in fetal sheep cardiomyocytes.","authors":"Natasha N Chattergoon, Karthikeyan Bose, Samantha Louey, Sonnet S Jonker","doi":"10.14814/phy2.70386","DOIUrl":"10.14814/phy2.70386","url":null,"abstract":"Fetal circulating lipids are low but rise precipitously following birth. It is unknown how prematurely elevated lipids affect the fetal heart, which primarily uses carbohydrates for energy. Fetal sheep were surgically instrumented and received Intralipid 20® or Lactated Ringer's Solution intravenously. After 8 days, myocardial biopsies were taken, and cardiomyocytes were dispersed. Lipid uptake was assessed by labeled saturated long-chain fatty acids (LCFA) and very long-chain fatty acids (VLCFA) incorporation. Maximal oxygen consumption rates (OCR) were measured. Gene and protein expression levels were measured by quantitative PCR and Western blotting. Intralipid treatment increased LCFA (p < 0.001) and VLCFA (p < 0.001) lipid droplet number, and LCFA (males p = 0.002) and VLCFA (p = 0.018) droplet size. Fetal Intralipid treatment reduced maximal OCR in basal media (p = 0.005). Palmitic acid decreased maximal OCR regardless of fetal treatment or length of in vitro exposure (p = 0.006). Fetal Intralipid upregulated genes included CD36 (p = 0.001), CPT1A (p < 0.001), CPT1B (p < 0.001), VLCAD (p < 0.001), and PDK4 (p < 0.001), with no differences in protein expression. There were no effects on ER stress, apoptosis, or autophagy markers. Extended elevated lipid levels in the fetus increased lipid uptake and may have shifted substrate preference towards lipids, but all lipid exposure depressed fetal cardiac metabolism. Prematurely elevated lipids mature but suppress oxidative metabolism.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 10","pages":"e70386"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12106950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feasibility of replacing ^99mTc-DTPA GFR measurements with eGFR from cystatin C in individuals with spinal cord injuries. 脊髓损伤患者用胱抑素C的eGFR替代99mTc-DTPA GFR测量的可行性

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70315

Tatiana Kristensen, Peter S Oturai, Bryan T Haddock, Fin Biering-Sørensen, Christina Kruuse, Ulrik B Andersen

{"title":"Feasibility of replacing 99mTc-DTPA GFR measurements with eGFR from cystatin C in individuals with spinal cord injuries.","authors":"Tatiana Kristensen, Peter S Oturai, Bryan T Haddock, Fin Biering-Sørensen, Christina Kruuse, Ulrik B Andersen","doi":"10.14814/phy2.70315","DOIUrl":"10.14814/phy2.70315","url":null,"abstract":"In individuals with spinal cord injury (SCI) and neurogenic bladder dysfunction, guidelines recommend regular monitoring of kidney function by measuring the glomerular filtration rate using an externally administered filtration markers such as 99mTc-DTPA, since creatinine-based eGFR models are inaccurate due to lower muscle mass in these individuals. To examine the feasibility of substituting GFR measurements with eGFR based on s-cystatin C, simultaneous 99mTc-DTPA clearance (mGFR) and cystatin C-based clearance (eGFRcys) measures were evaluated in 248 individuals with SCI. In a subgroup of 26 participants, the test-retest variability of eGFRcys was assessed. Finally, long-term (1-3 years) repeatability of simultaneously measured mGFR and eGFRcys was evaluated in 40 individuals. We could demonstrate a very good correlation between mGFR and eGFRcys, with an intraclass correlation (ICC) of 0.92, a very good test-retest variation of eGFRcys (ICC: 0.98) and a very good long-term repeatability of eGFRcys and mGFR (ICC 0.92 and 0.94, respectively). We conclude that in individuals with SCI, eGFR calculated from a single sample of cystatin C can replace measurements of GFR using an externally administered substance. Using a fixed normal limit rather than an age-corrected normal material for p-cystatin C or eGFRCYS will misclassify many individuals as having chronic kidney disease.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70315"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12069801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human macrophage pro-inflammatory polarization in response to free cholesterol and cholesterol remnants. 人巨噬细胞对游离胆固醇和胆固醇残留物的促炎极化反应。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70367

Paukner Karel, Muffova Barbora, Bartuskova Hana, Mareš Jan, Janousek Libor, Fronek Jiri, Kauerova Sona, Kralova Lesna Ivana, Poledne Rudolf

{"title":"Human macrophage pro-inflammatory polarization in response to free cholesterol and cholesterol remnants.","authors":"Paukner Karel, Muffova Barbora, Bartuskova Hana, Mareš Jan, Janousek Libor, Fronek Jiri, Kauerova Sona, Kralova Lesna Ivana, Poledne Rudolf","doi":"10.14814/phy2.70367","DOIUrl":"10.14814/phy2.70367","url":null,"abstract":"Atherosclerosis is a chronic inflammatory disease of the blood vessels caused by elevated levels of lipoproteins. The hyperlipoproteinemia triggers a series of cellular changes, particularly the activation of the macrophages, which play a crucial role in the development and progression of atherosclerosis. The presence of free cholesterol (FC) in lipoproteins may contribute to macrophage stimulation. However, the mechanisms linking the accumulation of FC in macrophages to their pro-inflammatory activation remain poorly understood. Our research found a positive correlation between the number of pro-inflammatory macrophages (CD14 + CD16 + CD36high) in visceral adipose tissue and the levels of LDL-C and cholesterol remnant particles in 56 healthy people. In contrast, the proportion of anti-inflammatory, alternatively activated macrophages (CD14 + CD16-CD163+) correlated negatively with HDL-C. Additionally, our in vitro study demonstrated that macrophages accumulating FC promoted a pro-inflammatory response, activating the TNF-α and chemokine CCL3 genes. Furthermore, the accumulation of FC in macrophages alters the surface receptors on macrophages (CD206 and CD16) and increases cellular granularity. Notably, the CD36 surface receptor and the ACAT and CD36 genes did not show a response. These results suggest a link between excessive FC accumulation and systemic inflammation to underlie the development of atherosclerosis.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 10","pages":"e70367"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanical properties of the gastrocnemius muscle-tendon unit in male athletic high jumpers. 男性跳高运动员腓肠肌-肌腱单元的力学特性。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70370

Toshihide Fujimori, Natsuki Sado

{"title":"Mechanical properties of the gastrocnemius muscle-tendon unit in male athletic high jumpers.","authors":"Toshihide Fujimori, Natsuki Sado","doi":"10.14814/phy2.70370","DOIUrl":"https://doi.org/10.14814/phy2.70370","url":null,"abstract":"Tendon compliance facilitates power exertion during stretch-shortening cycle exercises through muscle-tendon interaction. Tendons stiffen in response to mechanical loadings, and their stiffness sometimes affects motor performance, but no consensus has been reached yet. We investigated the gastrocnemius muscle-tendon properties of 10 male amateur high jumpers and 14 untrained males. Participants performed maximum voluntary contraction (MVC) of ankle plantar flexion. We measured the maximum joint torque and Achilles tendon stiffness using a torque dynamometer for force measurement, an ultrasound apparatus to track tendon elongation, and a motion capture system to correct joint rotation. High jumpers exerted significantly greater MVC torque than untrained individuals (152.8 ± 31.8 vs. 103.6 ± 18.9 Nm). Tendon stiffness did not significantly differ between groups (287.3 ± 90.9 vs. 258.4 ± 85.6 N/mm). This suggests that strengthening muscles and stiffening tendons may independently adapt through high jump training. In high jumpers, high jump personal best record significantly correlated with MVC torque (r = 0.73) but not significantly correlated with tendon stiffness (r = -0.07). Muscle force exertion ability enhanced by training should be important for improving high jump performance, while tendon stiffening is not necessary for performance. We suggest that humans may inherently have adequate tendon properties for jumping, even without specific training.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70370"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of extracellular matrix proteins on bovine fibro-adipogenic progenitor cell adhesion, proliferation, and differentiation in vitro. 细胞外基质蛋白对体外牛纤维脂肪祖细胞粘附、增殖和分化的影响。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70283

Perri Gish, Madison Stewart, Brandon Khuu, Nathaniel Meyer, Payam Vahmani, Lucas Smith

{"title":"The impact of extracellular matrix proteins on bovine fibro-adipogenic progenitor cell adhesion, proliferation, and differentiation in vitro.","authors":"Perri Gish, Madison Stewart, Brandon Khuu, Nathaniel Meyer, Payam Vahmani, Lucas Smith","doi":"10.14814/phy2.70283","DOIUrl":"https://doi.org/10.14814/phy2.70283","url":null,"abstract":"Fibro-adipogenic progenitor cells (FAPs) are mesenchymal stem cells that produce extracellular matrix (ECM) and intramuscular adipocytes in skeletal muscle. While FAPs have demonstrated responsiveness to their physical environment, there is limited knowledge of how the ECM substrate of FAPs impacts their differentiation, particularly in livestock animals. We hypothesized that the ECM substrate FAPs are cultured on will differentially impact their adherence, proliferation, and differentiation. Through an initial screen of 9 ECM proteins and their combinations, significant variation of bovine FAP attachment and differentiation across coatings was observed. The ECM substrates fibronectin, collagen 6, vitronectin, and a combination of fibronectin and collagen 6 were selected for further testing. Notably, fibronectin increased cell proliferation and attachment rates, without impairing FAP adipogenic or fibrogenic differentiation compared to the other coatings. Benefits of fibronectin were maintained at lower concentrations and when combined with less favorable coatings such as collagen 6. When assessed for their adipogenic potential on each coating at different substrate stiffnesses, lipid accumulation decreased with increasing substrate stiffness, while cell attachment increased on stiffer substrates. Overall, these results demonstrate the high responsiveness of FAPs to their ECM substrate, along with highlighting fibronectin as a preferred substrate for in vitro experiments with bovine FAPs.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70283"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12045701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of chronic exposure to biomass pollutants on cardiorespiratory responses and the occurrence of exercise-induced bronchoconstriction in healthy men. 慢性暴露于生物质污染物对健康男性心肺反应和运动性支气管收缩的影响

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70368

Pierre Lofuta Olenga Vuvu, Malgorzata Klass, Nathalie Pauwen, Augustin Mboko Kipula, Philippe van de Borne, Alain van Muylem, Silvia Perez-Bogerd, Gael Deboeck

{"title":"Effects of chronic exposure to biomass pollutants on cardiorespiratory responses and the occurrence of exercise-induced bronchoconstriction in healthy men.","authors":"Pierre Lofuta Olenga Vuvu, Malgorzata Klass, Nathalie Pauwen, Augustin Mboko Kipula, Philippe van de Borne, Alain van Muylem, Silvia Perez-Bogerd, Gael Deboeck","doi":"10.14814/phy2.70368","DOIUrl":"https://doi.org/10.14814/phy2.70368","url":null,"abstract":"Exposure to charcoal biomass (CB) pollutants affects the cardiorespiratory system. We assessed cardiopulmonary responses (CPR) to exercise in charcoal producers (CPs) compared to farmers and evaluated the prevalence of exercise-induced bronchoconstriction (EIB). Forty-five CPs and 36 farmers, healthy males aged 23-39, completed a 15-m Incremental Shuttle Walk and Run Test (15-m ISWRT). Air quality index (AQI) and CO intoxication were measured, CPR was assessed through heart rate (HR), blood pressures (SBP, DBP), and spirometry at rest, peak exercise, and during recovery at 5 and 15 min. Aerobic capacity (VO2 max) was estimated from the distance covered during the 15-m ISWRT, and EIB was defined as a >10% decrease in FEV1 from baseline values. AQI was worse in charcoal workplaces, and CPs had higher CO intoxication than farmers (p < 0.0001). Both groups reached maximal exercise %HRmax: 84 (82-89) versus 84 (80-89), p = 0.37 and showed similar predicted VO2 max 36.2 (31.1-43.1) versus 38.9 (32.2-43.7) mL/kg/min, p = 0.60. However, after ISWRT, CPs had lower FEV1 than farmers (2.9 ± 0.6 vs. 3.3 ± 0.6 L, p < 0.003) and slower recovery. EIB prevalence was higher in CPs (60.0% vs. 27.8%, p = 0.006). Chronic exposure to CB increases EIB in healthy CPs, suggesting heightened airway hyperreactivity.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70368"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mild hyperbaric oxygen enhances recovery of the plantaris muscle atrophy induced by cast immobilization of the hindlimb in male rats. 轻度高压氧促进雄性大鼠后肢固定后足底肌萎缩的恢复。

IF 2.2

Physiological Reports Pub Date : 2025-05-01 DOI: 10.14814/phy2.70350

Ai Takemura, Tatsuro Egawa, Ryo Takagi, Ryota Iyama, Zhao Haiyu, Shinichiro Suzuki, Reika Fujino, Takuya Fukunaga, Tatsuya Hayashi, Satoshi Fujita

{"title":"Mild hyperbaric oxygen enhances recovery of the plantaris muscle atrophy induced by cast immobilization of the hindlimb in male rats.","authors":"Ai Takemura, Tatsuro Egawa, Ryo Takagi, Ryota Iyama, Zhao Haiyu, Shinichiro Suzuki, Reika Fujino, Takuya Fukunaga, Tatsuya Hayashi, Satoshi Fujita","doi":"10.14814/phy2.70350","DOIUrl":"https://doi.org/10.14814/phy2.70350","url":null,"abstract":"Loss of muscle mass is associated with muscle functional decline and mortality. The present study aimed to determine whether exposure to mild hyperbaric oxygen (MHO) during and after casting immobilization reduces muscle atrophy. We distributed eight-week-old rats into control (CON), cast immobilization (Cast), and Cast + MHO (1.3 atmosphere absolute with 38% oxygen) groups. Rats were cast for 2 weeks under the normal or MHO condition, followed by a two-week recovery period under the same condition after cast removal. The plantaris muscle weight (mg/g BW) decreased by approximately 11.5% in the Cast group compared to the CON group (p < 0.01), while there were no differences between the CON and Cast + MHO groups, suggesting that MHO enhanced the recovery of muscle atrophy. However, the soleus muscle weight (mg/g BW) decreased by casting immobilization, regardless of MHO. The enzyme activity by succinate dehydrogenase (SDH) staining in the plantaris muscle was lower in the Cast group than in the CON group (p < 0.01), while there were no differences between the CON and Cast + MHO groups. In summary, MHO enhances the recovery of plantaris muscle atrophy and partially attenuates the decreased SDH activity after cast immobilization of hindlimb in rats.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 9","pages":"e70350"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12051368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144047949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0