Physiological Reports最新文献

Quantification of training-induced alterations in body composition via automated machine learning analysis of MRI images in the thigh region: A pilot study in young females.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70187

Saied Ramedani, Ebru Kelesoglu, Norman Stutzig, Hendrik Von Tengg-Kobligk, Keivan Daneshvar Ghorbani, Tobias Siebert

{"title":"Quantification of training-induced alterations in body composition via automated machine learning analysis of MRI images in the thigh region: A pilot study in young females.","authors":"Saied Ramedani, Ebru Kelesoglu, Norman Stutzig, Hendrik Von Tengg-Kobligk, Keivan Daneshvar Ghorbani, Tobias Siebert","doi":"10.14814/phy2.70187","DOIUrl":"https://doi.org/10.14814/phy2.70187","url":null,"abstract":"The maintenance of an appropriate ratio of body fat to muscle mass is essential for the preservation of health and performance, as excessive body fat is associated with an increased risk of various diseases. Accurate body composition assessment requires precise segmentation of structures. In this study we developed a novel automatic machine learning approach for volumetric segmentation and quantitative assessment of MRI volumes and investigated the efficacy of using a machine learning algorithm to assess muscle, subcutaneous adipose tissue (SAT), and bone volume of the thigh before and after a strength training. Eighteen healthy, young, female volunteers were randomly allocated to two groups: intervention group (IG) and control group (CG). The IG group followed an 8-week strength endurance training plan that was conducted two times per week. Before and after the training, the subjects of both groups underwent MRI scanning. The evaluation of the image data was performed by a machine learning system which is based on a 3D U-Net-based Convolutional Neural Network. The volumes of muscle, bone, and SAT were each examined using a 2 (GROUP [IG vs. CG]) × 2 (TIME [pre-intervention vs. post-intervention]) analysis of variance (ANOVA) with repeated measures for the factor TIME. The results of the ANOVA demonstrate significant TIME × GROUP interaction effects for the muscle volume (F1,16 = 12.80, p = 0.003, ηP 2 = 0.44) with an increase of 2.93% in the IG group and no change in the CG (-0.62%, p = 0.893). There were no significant changes in bone or SAT volume between the groups. This study supports the use of artificial intelligence systems to analyze MRI images as a reliable tool for monitoring training responses on body composition.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70187"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and function of Connexin 43 and Connexin 37 in the murine zona glomerulosa.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70215

Gabriel Stölting, Nicole Hellmig, Hoang An Dinh, Frederike Butz, Ali Kerim Secener, Marina Volkert, Ute I Scholl

{"title":"Expression and function of Connexin 43 and Connexin 37 in the murine zona glomerulosa.","authors":"Gabriel Stölting, Nicole Hellmig, Hoang An Dinh, Frederike Butz, Ali Kerim Secener, Marina Volkert, Ute I Scholl","doi":"10.14814/phy2.70215","DOIUrl":"https://doi.org/10.14814/phy2.70215","url":null,"abstract":"The zona glomerulosa (ZG) synthesizes the mineralocorticoid aldosterone. The primary role of aldosterone is the maintenance of volume and electrolyte homeostasis. Aldosterone synthesis is primarily regulated via tightly controlled oscillations in intracellular calcium levels in response to stimulation. It has previously been shown that calcium oscillations are synchronized through mechanical linkage between adjacent ZG cells. In many other cell types, similar synchronization is rather dependent on gap junctions (GJ). The recent discovery of mutations in CADM1 was linked to impaired GJ function in the ZG. Based on published transcriptomics data, we re-examined the presence and functional impact of GJ in the ZG. We found evidence for the expression of murine connexin 43 and 37 using microarray data, in-situ hybridization and immunohistology. Connexin 43 was also present in human samples. Calcium oscillations in ZG rosettes showed some degree of synchronization as reported previously. Unspecific GJ inhibition only had a small impact on this synchronicity. However, no signs of connections between cytosols could be observed as indicated by the lack of fluorescence recovery after photobleaching. We conclude that, while connexin proteins are expressed in the ZG, functional GJ in the physiological ZG are rare and of little consequence for calcium signaling.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70215"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prolonged sitting is not associated with altered shear-mediated dilation of the internal carotid artery, despite impairing lower limb endothelial function.

IF 2.2

Physiological Reports Pub Date : 2025-02-01 DOI: 10.14814/phy2.70097

Shotaro Saito, Kento Dora, Marino Karaki, Narumi Kunimatsu, Hayato Tsukamoto, Jun Sugawara, Erika Iwamoto, Shigehiko Ogoh

{"title":"Prolonged sitting is not associated with altered shear-mediated dilation of the internal carotid artery, despite impairing lower limb endothelial function.","authors":"Shotaro Saito, Kento Dora, Marino Karaki, Narumi Kunimatsu, Hayato Tsukamoto, Jun Sugawara, Erika Iwamoto, Shigehiko Ogoh","doi":"10.14814/phy2.70097","DOIUrl":"https://doi.org/10.14814/phy2.70097","url":null,"abstract":"The present study aims to examine the effect of 4 h of continuous sitting on cerebral endothelial function, which is a crucial component of cerebral blood flow regulation. We hypothesized that 4 h of sitting may impair cerebral endothelial function similarly to how it affects lower limb vasculature. Thirteen young, healthy participants were instructed to remain seated for 4 h without moving their lower limbs. The blood flow and shear rate (SR) in the popliteal and internal carotid artery (ICA) were measured using duplex Doppler ultrasound. During the 4-h sitting, peripheral (popliteal artery) and cerebral (ICA) endothelial function were assessed every hour. We induced peripheral and cerebral flow-mediated dilation (pFMD and ICA FMD) using hyperemia (5 min of cuff inflation on lower limb, then deflation) or hypercapnia (30s of hypercapnia, end-tidal partial pressure of CO2 + 9 mmHg), respectively. We then calculated each relative peak dilation from the baseline diameter to identify both pFMD and ICA FMD. We observed a significant decrease in pFMD starting at 2 h from the onset of sitting, and this reduction persisted throughout the 4-h sitting [Base (6.8 ± 4.2%) vs. 2-h (3.9 ± 2.0%), p = 0.044; vs. 3-h (3.2 ± 1.8%), p = 0.016; vs. 4-h (3.2 ± 1.9%), p = 0.005]. In contrast, during the 4-h sitting, ICA blood flow, SR, and ICA FMD remained unchanged (p = 0.062, p = 0.068, and p = 0.203, respectively). Unlike peripheral endothelial function, cerebral endothelial function remained stable during 4-h sitting. This suggests that the acute effect of prolonged sitting on cerebral vasculature differs from that of lower limb vasculature.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 3","pages":"e70097"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral administration of pioglitazone inhibits pulmonary hypertension by regulating the gut microbiome and plasma metabolome in male rats. 口服吡格列酮通过调节肠道微生物组和血浆代谢组抑制雄性大鼠肺动脉高压。

IF 2.2

Physiological Reports Pub Date : 2025-01-01 DOI: 10.14814/phy2.70174

Zizhou Zhang, Yaru Liang, Shaocong Mo, Mingming Zhao, Yi Li, Chenting Zhang, Xiaoqian Shan, Shiyun Liu, Jing Liao, Xiaoyun Luo, Junqi Zhu, Chen Wang, Qian Jiang, Chi Hou, Wei Hong, Ning Lai, Yuqin Chen, Lei Xu, Wenju Lu, Jian Wang, Zhongfang Wang, Kai Yang

{"title":"Oral administration of pioglitazone inhibits pulmonary hypertension by regulating the gut microbiome and plasma metabolome in male rats.","authors":"Zizhou Zhang, Yaru Liang, Shaocong Mo, Mingming Zhao, Yi Li, Chenting Zhang, Xiaoqian Shan, Shiyun Liu, Jing Liao, Xiaoyun Luo, Junqi Zhu, Chen Wang, Qian Jiang, Chi Hou, Wei Hong, Ning Lai, Yuqin Chen, Lei Xu, Wenju Lu, Jian Wang, Zhongfang Wang, Kai Yang","doi":"10.14814/phy2.70174","DOIUrl":"10.14814/phy2.70174","url":null,"abstract":"The oral administrated thiazolidinediones (TZDs) have been widely reported to alleviate experimental pulmonary hypertension (PH). However, previous studies mainly focused on their beneficial effects on the cardiopulmonary vascular system but failed to determine their potential roles on gut microenvironment. This study aims to investigate the effects of pioglitazone, an oral TZD drug, on gut microbiome in classic PH rat models induced by hypoxia (HPH) or SU5416/hypoxia (SuHx-PH) and evaluate the therapeutic potential of supplementation of selective probiotics for experimental PH. Pioglitazone remarkably inhibited the PH pathogenesis in both models and reshaped the gut microbiome and plasma metabolome. Correlation analyses represented strong and unique association between the protective metabolites and bacteria genera (Roseburia, Lactobacillus, and Streptococcus) that were positively stimulated by pioglitazone. Supplementation of selective probiotics Roseburia intestinalis (R. intestinalis) partially attenuated SuHx-PH and rebuilt a novel gut microbiome and host metabolome. This study reports for the first time that oral administration of pioglitazone protects PH by regulating the gut microbiome and host metabolome, providing novel insights for the TZD drugs. The data also supports that modulation of gut microbiota by supplementation of selective probiotics could be a novel effective therapeutic strategy for the treatment of PH.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 1","pages":"e70174"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11685845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigations of segmental arterial stiffness in a cross-sectional study on young adult male trained swimmers, cyclists, and non-trained men. 在一项针对年轻成年男性游泳运动员、骑自行车者和未受过训练的男性的横断面研究中对动脉节段性僵硬度的调查。

IF 2.2

Physiological Reports Pub Date : 2025-01-01 DOI: 10.14814/phy2.70186

Masato Nishiwaki, Daisuke Kume, Naoyuki Matsumoto

{"title":"Investigations of segmental arterial stiffness in a cross-sectional study on young adult male trained swimmers, cyclists, and non-trained men.","authors":"Masato Nishiwaki, Daisuke Kume, Naoyuki Matsumoto","doi":"10.14814/phy2.70186","DOIUrl":"10.14814/phy2.70186","url":null,"abstract":"Contrary to cardiovascular risk reductions by aerobic exercise, arterial stiffness, as assessed by brachial-ankle pulse wave velocity (PWV), is higher in swimmers and controls than in other aerobically trained individuals. The main muscles actively recruited in swimming are in the upper limbs, so this study aimed to investigate heart-brachial PWV in swimmers and to compare arterial stiffness indices between modes and measurement localities. Subjects comprised 60 individuals (18-22 years), including 20 untrained controls (Con), 20 aerobically trained cyclists (Aero), and 20 swimmers (Swim). Characteristics and strength did not differ, but peak oxygen uptake was significantly higher in Aero and Swim than in Con. Brachial-ankle PWV was significantly lower in Aero than in Con and Swim and no significant difference was observed between Con and Swim (Con, 1070 ± 115; Aero, 916 ± 109; Swim, 1035 ± 91 cm/s). Nevertheless, heart-brachial PWV was significantly lower in Swim than in Con and tended to be lower in Swim than in Aero (Con, 344 ± 25; Aero, 330 ± 41; Swim, 308 ± 31 cm/s). Heart-ankle PWV was significantly lower in both Swim and Aero than in Con (Con, 618 ± 47; Aero, 580 ± 54; Swim, 576 ± 43 cm/s). Therefore, these findings indicate that swimmers can develop segment-specific reductions in heart-brachial arterial stiffness, unlike aerobically trained cyclists.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 1","pages":"e70186"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11693416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of PKM2 dysregulates inflammatory signaling in the infarcted murine heart. PKM2缺失会使梗死小鼠心脏的炎症信号失调。

IF 2.2

Physiological Reports Pub Date : 2025-01-01 DOI: 10.14814/phy2.70193

Katie C Y Lee, Allison L Williams, Akitoshi Hara, Vedbar S Khadka, Jeffrey Hayashi, Ralph V Shohet

{"title":"Loss of PKM2 dysregulates inflammatory signaling in the infarcted murine heart.","authors":"Katie C Y Lee, Allison L Williams, Akitoshi Hara, Vedbar S Khadka, Jeffrey Hayashi, Ralph V Shohet","doi":"10.14814/phy2.70193","DOIUrl":"https://doi.org/10.14814/phy2.70193","url":null,"abstract":"Inflammation and a metabolic shift from oxidative metabolism to glycolysis are common in the ischemic heart, the latter partly controlled by pyruvate kinase (muscle, PKM). We previously identified alternative splicing promoting the PKM2 isoform after myocardial infarction (MI). We examined the role of PKM2 physiological upregulation after MI, modeled by ligation of the left anterior descending coronary artery, using global PKM2 knockout (PKM2-/-) mice. Echocardiography showed similar cardiac function between PKM2-/- and control mice after MI. However, PKM2-/- infarcted hearts had increased abundances of transcripts associated with oxidative stress and immune responses. Immunohistochemistry revealed greater abundance of macrophages in PKM2-/- hearts prior to MI, with a small increase in CD86+ macrophages in PKM2-/- infarcted hearts. Elevated baseline plasma IL-6, IL-1β, and C-reactive protein, and cardiac IL-6, 3 days post-MI, were observed in PKM2-/- mice. Oxidative lipid products were also elevated in baseline PKM2-/- hearts, while antioxidant glutathione peroxidase 4 was reduced. Greater fibrosis was seen in PKM2-/- hearts 28 days after MI. These findings suggest Pkm2 ablation primes the heart for increased oxidative stress, inflammation, and fibrosis post-MI. The natural upregulation of PKM2 may mitigate fibrosis by reducing oxidative stress and inflammation, highlighting its protective role in the infarcted heart.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 1","pages":"e70193"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705480/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does mental exertion during incremental exercise change substrate oxidation and cardiorespiratory outcomes in individuals with overweight? 增加运动时的脑力消耗是否会改变超重个体的底物氧化和心肺结局？

IF 2.2

Physiological Reports Pub Date : 2025-01-01 DOI: 10.14814/phy2.70172

Samira Pourmirzaei Kouhbanani, Seyed Kamaledin Setarehdan, Rana Fayazmilani

{"title":"Does mental exertion during incremental exercise change substrate oxidation and cardiorespiratory outcomes in individuals with overweight?","authors":"Samira Pourmirzaei Kouhbanani, Seyed Kamaledin Setarehdan, Rana Fayazmilani","doi":"10.14814/phy2.70172","DOIUrl":"10.14814/phy2.70172","url":null,"abstract":"Given the growing concern over the impact of brain health in individuals with overweight, understanding how mental exertion (ME) during exercise affects substrate oxidation and cardiorespiratory outcomes is crucial. This study examines how ME impacts these outcomes during an incremental exercise test in adults with overweight. Seventeen adults who were overweight completed an incremental exercise test on a cycle ergometer two times, with and without the Stroop task. Energy expenditure (EE), carbohydrate and fat oxidation, maximum heart rate (HRmax), maximal oxygen uptake (VO2max), maximum fat oxidation (MFO), and the intensity of exercise that elicited MFO (Fatmax) are measured by indirect calorimetry. ME did not change the EE, carbohydrate, and fat oxidation at any stages of the incremental test. However, ME resulted in significantly lower HRmax, VO2max, and MFO (p < 0.01) and increased NASA-TLX scores but showed no change in Fatmax. These results show ME decreases the value of HRmax, VO2max, and MFO during the incremental exercise test. Due to the increased mental workload demonstrated by the NASA-TLX test, adults with overweight are unable to complete the test to the same extent as they did in the test without ME according to maximal levels in this study.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 1","pages":"e70172"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between skin sympathetic nerve activity and electrocardiogram alterations after subarachnoid hemorrhage. 蛛网膜下腔出血后皮肤交感神经活动与心电图改变的关系。

IF 2.2

Physiological Reports Pub Date : 2025-01-01 DOI: 10.14814/phy2.70202

Yoichiro Nakagawa, Takashi Kusayama, Sho Tamai, Yuta Nagamori, Kazutaka Takeuchi, Shuhei Iwaisako, Taishi Tsutsui, Tomoya Kamide, Kouichi Misaki, Soichiro Usui, Kenji Sakata, Mitsutoshi Nakada, Masayuki Takamura

{"title":"Association between skin sympathetic nerve activity and electrocardiogram alterations after subarachnoid hemorrhage.","authors":"Yoichiro Nakagawa, Takashi Kusayama, Sho Tamai, Yuta Nagamori, Kazutaka Takeuchi, Shuhei Iwaisako, Taishi Tsutsui, Tomoya Kamide, Kouichi Misaki, Soichiro Usui, Kenji Sakata, Mitsutoshi Nakada, Masayuki Takamura","doi":"10.14814/phy2.70202","DOIUrl":"10.14814/phy2.70202","url":null,"abstract":"While autonomic dysregulation and repolarization abnormalities are observed in subarachnoid hemorrhage (SAH), their relationship remains unclear. We aimed to measure skin sympathetic nerve activity (SKNA), a novel method to estimate stellate ganglion nerve activity, and investigate its association with electrocardiogram (ECG) alterations after SAH. We recorded a total of 179 SKNA data from SAH patients at three distinct phases and compared them with 20 data from controls. Compared with control data, in the acute phase data (days 1-3 of SAH), T peak-to-end (Tp-e) interval was significantly prolonged (81 [interquartile range {IQR}: 71-93] vs. 58 [IQR: 54-64] ms, p < 0.001), non-burst amplitude of SKNA was significantly increased (2.4 [IQR: 1.3-4.1] vs. 0.7 [IQR: 0.5-1.7] μV, p < 0.001), and the ratio of low frequency to high frequency (HF) was significantly decreased (0.8 [IQR: 0.5-1.1] vs. 1.1 [IQR: 0.7-1.3], p = 0.028). Linear mixed model revealed a relationship between Tp-e interval and SKNA. Although these abnormalities gradually normalized, delayed cerebral ischemia episodes were associated with increased HF oscillation. Transient sympathetic dysregulation contributes to repolarization impairment after SAH. SKNA may have the potential to monitor adverse outcomes.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 1","pages":"e70202"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miRNA-1 regulation is necessary for mechanical overload-induced muscle hypertrophy in male mice. miRNA-1调控是雄性小鼠机械负荷引起的肌肉肥大所必需的。

IF 2.2

Physiological Reports Pub Date : 2025-01-01 DOI: 10.14814/phy2.70166

Shengyi Fei, Blake D Rule, Joshua S Godwin, C Brooks Mobley, Michael D Roberts, Ferdinand von Walden, Ivan J Vechetti

{"title":"miRNA-1 regulation is necessary for mechanical overload-induced muscle hypertrophy in male mice.","authors":"Shengyi Fei, Blake D Rule, Joshua S Godwin, C Brooks Mobley, Michael D Roberts, Ferdinand von Walden, Ivan J Vechetti","doi":"10.14814/phy2.70166","DOIUrl":"https://doi.org/10.14814/phy2.70166","url":null,"abstract":"MicroRNAs (miRNAs) are small, noncoding RNAs that play a critical role in regulating gene expression post-transcriptionally. They are involved in various developmental and physiological processes, and their dysregulation is linked to various diseases. Skeletal muscle-specific miRNAs, including miR-1, play a crucial role in the development and maintenance of skeletal muscle. It has been demonstrated that the expression of miR-1 decreases by approximately 50% in response to hypertrophic stimuli, suggesting its potential involvement in muscle hypertrophy. In our study, we hypothesize that reduction of miR-1 levels is necessary for skeletal muscle growth due to its interaction to essential pro-growth genes. Promoting a smaller reduction of miR-1 levels, we observed a blunted hypertrophic response in mice undergoing a murine model of muscle hypertrophy. In addition, our results suggest that miR-1 inhibits the expression of Itm2a, a membrane-related protein, as potential miR-1-related candidate for skeletal muscle hypertrophy. While the exact mechanism in muscle hypertrophy has not been identified, our results suggest that miR-1-regulated membrane proteins are important for skeletal muscle hypertrophy.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 1","pages":"e70166"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of a combined long-duration static stretching and resistance training regimen on a competitive bodybuilder: A case study. 结合长时间静态拉伸和阻力训练方案对竞技健美运动员的影响：一个案例研究。

IF 2.2

Physiological Reports Pub Date : 2025-01-01 DOI: 10.14814/phy2.70156

Kai A Homer, Eric R Helms, Alyssa-Joy Spence

{"title":"The effect of a combined long-duration static stretching and resistance training regimen on a competitive bodybuilder: A case study.","authors":"Kai A Homer, Eric R Helms, Alyssa-Joy Spence","doi":"10.14814/phy2.70156","DOIUrl":"https://doi.org/10.14814/phy2.70156","url":null,"abstract":"Both resistance training (RT) and long-duration, high-intensity stretching induce muscular adaptations; however, it is unknown whether the modalities are complementary or redundant, particularly in well-trained individuals. A case-study was conducted on a competitive bodybuilder implementing long-duration, high-intensity stretching of the plantar flexors (60 min 6x/week for 12 weeks) in conjunction with their habitual RT. Ultrasound muscle architecture (muscle thickness [MT], fascicle length [FL], and pennation angle [PA]) measurements were collected at multiple sites at four weekly baseline sessions, six (mid) and 12 (post1) weeks following the commencement of the intervention, and a week after the intervention (post2) while isometric strength and range of motion (RoM) were obtained once at baseline, mid, post1, and post2. 2SD band plots were constructed to determine meaningful changes in MT, FL, and PA from the four baseline measures while percentage and absolute change across each timepoint were calculated for all variables. From baseline to post 1, RoM, strength, and MT increased 25.9%, 11.4%, and 7.4%-23.4%, respectively, while four MT and two PA sites exceeded the threshold for meaningful change. The combined stretching and RT protocols resulted in flexibility, strength, and MT adaptations; however, findings should be generalized with caution given the case-study nature of our investigation.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 2","pages":"e70156"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0