Physiological Reports最新文献

Quantifying the effects of hemoglobin saturation on plasma strong ion difference during blood oxygenation and decarboxylation. 定量血红蛋白饱和度对血氧和脱羧过程中血浆强离子差的影响。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71118

Lorenzo Giosa, Martin Krbec, Jakub Halamík, Adrian Bolnberger, Mattia Busana, Serena Brusatori, Tue Diemer, Søren R Kristensen, Micah L A Heldeweg, Francesco Zadek, Stephen E Rees, František Duška

{"title":"Quantifying the effects of hemoglobin saturation on plasma strong ion difference during blood oxygenation and decarboxylation.","authors":"Lorenzo Giosa, Martin Krbec, Jakub Halamík, Adrian Bolnberger, Mattia Busana, Serena Brusatori, Tue Diemer, Søren R Kristensen, Micah L A Heldeweg, Francesco Zadek, Stephen E Rees, František Duška","doi":"10.14814/phy2.71118","DOIUrl":"10.14814/phy2.71118","url":null,"abstract":"The strong ion difference (SID) is assessed to interpret acid-base disorders, yet measured plasma values are influenced by electrolyte redistribution across compartments. Current models focus on pH-dependent plasma-erythrocyte shifts. Here, we aim to quantify the effects of hemoglobin oxygen saturation (sO2). We induced oxygenation and decarboxylation of human venous blood (n = 20) via room-air equilibration. We modeled the contribution of ∆sO2 to plasma-erythrocyte shifts through the Haldane effect and hypothesized that combining sO2- with pH-dependent mechanisms would allow accurate prediction of redistribution related ∆SID. After room-air equilibration, sO2 fraction increased by 0.46 [0.39-0.54], CO2 tension decreased by 29 [25-32] mmHg, and pH increased by 0.25 [0.19-0.32]. SID decreased by 5.3 [4.2 to 5.6] mEq/L, and its changes were independently associated with ∆sO2 (∆SID/∆sO2 = -3.0 [-5.4 to -0.67] mEq/L, p < 0.01). Accordingly, when only pH-dependent redistribution was considered, ∆SID prediction yielded a ∆sO2-dependent (p < 0.01) underestimation of measured ∆SID (mean bias [limits of agreement]: -1.6 [-3.7 to 0.5] mEq/L). Including sO2-dependent effects improved the bias (-0.4 [-2.0 to 1.3] mEq/L) and removed its ∆sO2-dependence (p = 0.63), and this result was maintained using a simplified model (∆SID = 1.5·[Hemoglobin+Albumin]g/dL·∆pH+[Hemoglobin/4]g/dL·∆sO2). We conclude that ∆sO2 independently affects ∆SID during blood oxygenation and decarboxylation. Incorporating sO2- alongside pH-dependent electrolyte shifts enables accurate prediction of redistribution-related ∆SID.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 19","pages":"e71118"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13623668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does a low-carb, high-fat diet change muscle insulin receptor concentration and body composition in a swine biomedical model? 在猪生物医学模型中，低碳水化合物、高脂肪饮食会改变肌肉胰岛素受体浓度和身体成分吗？

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71124

Natalie Acosta-Castellanos, Shelly Tiede, Christopher J Byrd, Pawel Borowicz, Jennifer M Young, Eric P Berg

{"title":"Does a low-carb, high-fat diet change muscle insulin receptor concentration and body composition in a swine biomedical model?","authors":"Natalie Acosta-Castellanos, Shelly Tiede, Christopher J Byrd, Pawel Borowicz, Jennifer M Young, Eric P Berg","doi":"10.14814/phy2.71124","DOIUrl":"10.14814/phy2.71124","url":null,"abstract":"Our objective was to evaluate body composition and muscle insulin receptor concentration resulting from consumption of a low-carbohydrate, high-fat diet using swine as a surrogate for humans. Two treatments, a control standard swine feed (CON; n = 7) and test diet where beef tallow replaced the corn carbohydrate (LCHF; n = 8), were fed for 32 days to post-pubertal gilts (Landrace × Yorkshire). Body weight (BW), subcutaneous fat (FD), daily gain (ADG), daily feed intake (ADFI), and fasting blood chemistry were measured. Organ weights, and pancreas, longissimus, and semimembranosus samples were collected from three gilts per treatment. LCHF gilts had reduced ADG, ADFI, FD, and visceral fat (p = 0.09, 0.04, 0.06, and 0.03; respectively) with similar BW (p = 0.18). Blood chemistry parameters were not affected but for fasting glucose, which tended to be higher for LCHF (p = 0.054). The percentage (p = 0.09) and number (p = 0.07) of active pancreatic β-cells tended to be higher for LCHF gilts, as was the concentration of semimembranosus insulin receptors (p = 0.04). Short-term consumption of a low-carb, high-fat diet improved body composition, insulin production mechanics, and muscle insulin receptor density in a swine biomedical model.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 19","pages":"e71124"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13623663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altitude thresholds for hemoglobin and plasma volume regulation in adult residents of different altitudes and their impact on hemoglobin concentration. 不同海拔地区成人血红蛋白和血浆容量调节的海拔阈值及其对血红蛋白浓度的影响。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71108

Edgar Cristancho-Mejía, Andrea Catalina Trompetero-Gonzalez, William Fernando Benavides-Pinzón, Walter Franz-Joachim Schmidt

{"title":"Altitude thresholds for hemoglobin and plasma volume regulation in adult residents of different altitudes and their impact on hemoglobin concentration.","authors":"Edgar Cristancho-Mejía, Andrea Catalina Trompetero-Gonzalez, William Fernando Benavides-Pinzón, Walter Franz-Joachim Schmidt","doi":"10.14814/phy2.71108","DOIUrl":"https://doi.org/10.14814/phy2.71108","url":null,"abstract":"Life at altitude is characterized by increased erythropoietic activity and hemoglobin concentration ([Hb]). The first aim of the project was to identify the altitudes at which significant increases in hemoglobin mass (Hbmass) and decreases in plasma volume (PV) occur among residents living at different altitudes. The second aim was to evaluate the extent to which Hbmass and PV contribute to changes in [Hb] at moderate altitudes. The study included 133 young men and 148 women from six Andean regions at altitudes ranging from 970 to 2600 m. Hbmass was determined using carbon monoxide rebreathing. PV was calculated using Hbmass, [Hb], and hematocrit. Hbmass increased up to 2600 m by 35.9 ± 11.6 g, while PV decreased by 208 ± 53 mL (both p < 0.01). The first significant increase in Hbmass occurred at 2180 m and the first significant decrease in PV occurred at 1730 m. At 2600 m, Hbmass and PV contributed equally to the 1.2 g/dL increase in [Hb], that is, 0.56 and 0.61 g/dL, respectively. The threshold for a decrease in PV in Andeans is ~450 m lower than the threshold for an increase in Hbmass. The contribution of hemoconcentration to the increase in [Hb] at moderate altitudes is equal to the contribution of erythropoiesis.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 19","pages":"e71108"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13633473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149113766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adding Tat-Beclin1 to an endurance training program promotes additive improvements in physical function in aged male mice. 将Tat-Beclin1添加到耐力训练计划中，可以促进老年雄性小鼠身体功能的附加改善。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71128

Thuan Thien Tchen, Shakibur Rahman, Thaysa Ghiarone, Lynn A Spruce, Hossein Fazelinia, Elizabeth M Brown, Charalampos Papachristou, Sue C Bodine, Vitor A Lira, Kleiton A S Silva

{"title":"Adding Tat-Beclin1 to an endurance training program promotes additive improvements in physical function in aged male mice.","authors":"Thuan Thien Tchen, Shakibur Rahman, Thaysa Ghiarone, Lynn A Spruce, Hossein Fazelinia, Elizabeth M Brown, Charalampos Papachristou, Sue C Bodine, Vitor A Lira, Kleiton A S Silva","doi":"10.14814/phy2.71128","DOIUrl":"10.14814/phy2.71128","url":null,"abstract":"Autophagy is a hallmark of aging, but it has not been exclusively targeted to attenuate aging-related physical dysfunction. Here, we combined Tat-Beclin1 and endurance training to determine its effect on physical function domains in aged male mice. Mice were randomly divided into Control (N = 5), Tat-Beclin1 (TB, 15 mg/kg, 2×/week, N = 9), Exercise (Exe, 70%-maximal-running-speed, 3×/week, N = 9), and TB+Exe (N = 8) for 1 month in 23-month-old male C57BL/6J mice. Animals were assessed for grip strength (GS), endurance capacity (EC) on a treadmill, and balance and coordination on a rotarod. Gastrocnemius/plantaris (G/P) and tibialis anterior muscles were harvested for western blotting, myofiber typing, and proteomic profiling (G/P only). Although no significant interaction was observed, the TB+Exe group showed improvements in GS, EC, and balance and coordination performance, an outcome comparable with additive effects of both therapies. Limited changes were observed in myofiber typing and autophagy markers among groups. A proteomic analysis revealed that TB upregulated biological processes involved in muscle contraction, whereas TB+Exe surprisingly upregulated acute inflammatory responses, including proteins such as haptoglobin and orosomucoid-1. Altogether, combining TB and endurance training elicits additive effects on physical functions of aged male mice and may represent a new approach to attenuate aging-related physical dysfunction.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 19","pages":"e71128"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13652958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149149037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exercise training does not mitigate high-phosphate diet-induced mitochondrial dysfunction in skeletal muscle in male mice. 运动训练不能减轻高磷酸盐饮食引起的雄性小鼠骨骼肌线粒体功能障碍。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71139

Hannah F Sanders, Dominic W Kolonay, Jade A Blackwell, Jiaru Liu, Isabella R Dobrski, Daniel A Branch, Lucas A Lefers, Benjamen H Kern, Kedryn K Baskin

{"title":"Exercise training does not mitigate high-phosphate diet-induced mitochondrial dysfunction in skeletal muscle in male mice.","authors":"Hannah F Sanders, Dominic W Kolonay, Jade A Blackwell, Jiaru Liu, Isabella R Dobrski, Daniel A Branch, Lucas A Lefers, Benjamen H Kern, Kedryn K Baskin","doi":"10.14814/phy2.71139","DOIUrl":"https://doi.org/10.14814/phy2.71139","url":null,"abstract":"Inorganic phosphate consumption in adults is two-to-three-fold higher than the recommended daily allowance due to its widespread use as a preservative and flavor enhancer in processed foods. We previously reported that 12 weeks of high-phosphate diet (HPD) reduced VO2max and altered metabolic gene expression and mitochondrial dysfunction in mouse skeletal muscle. Here, we investigated whether exercise training reverses the negative consequences of HPD. Male mice were fed normal (NPD) or HPD for 5 weeks, then continued on NPD or HPD with 9 weeks of voluntary exercise training. Serum, urine, and fecal phosphate increased within 1 day of HPD-feeding and remained elevated throughout the study. Trained NPD- and HPD-fed mice performed similarly on forced exercise tests, but mitochondrial respiration was significantly decreased in skeletal muscle from trained HPD-fed mice. RNA sequencing revealed distinct sets of differentially expressed genes when comparing sedentary versus exercise-trained muscle of HPD-fed mice relative to the same comparison in NPD-fed mice. These findings indicate that voluntary exercise training preserves endurance but does not fully restore gene expression or mitochondrial function in muscle from HPD-fed mice. Thus, HPD-induced skeletal muscle dysregulation is not completely reversible with exercise, underscoring the importance of both diet and exercise for metabolic health.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 20","pages":"e71139"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EXPRESSION OF CONCERN: BReast CAncer susceptibility gene 2 deficiency exacerbates oxidized LDL-induced DNA damage and endothelial apoptosis. 关注表达：乳腺癌易感基因2缺乏加剧氧化ldl诱导的DNA损伤和内皮细胞凋亡。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71121

引用次数: 0

Beyond canonical homeostasis: Dynamic, plastic, and intermittently unregulated physiology as the norm. 超越规范的内稳态：作为规范的动态、可塑和间歇性不受调节的生理。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71150

Warren Burggren, Tobis Wang

{"title":"Beyond canonical homeostasis: Dynamic, plastic, and intermittently unregulated physiology as the norm.","authors":"Warren Burggren, Tobis Wang","doi":"10.14814/phy2.71150","DOIUrl":"https://doi.org/10.14814/phy2.71150","url":null,"abstract":"Homeostasis is often taught as steady-state regulation keeping internal variables within narrow limits-reflecting a mammalian bias. Yet, canonical homeostasis is far from universal. In many animals, including humans, normal physiological states allow some variables to fluctuate cyclically or stochastically while others remain tightly controlled. Indeed, across non-mammalian species, broad physiological variability is frequently normal rather than exceptional. Here we present case studies showing that plastic, variable regulation-sometimes approaching randomness-is both common and adaptive. Examples include intermittent breathing during diving, episodic feeding and digestion, pulsatile hormone release, variable sleep cycles, irregular gastric emptying, fluctuating renal blood flow, and stochastic immune responses. These complex regulatory patterns represent evolved strategies-not failures of homeostasis-allowing organisms to function efficiently in dynamic environments. Such flexible regulation, including unregulated intervals, may minimize energetic costs compared with continuous tight control, enhancing evolutionary fitness. We propose that variable degrees of physiological regulation have evolved as energy-efficient solutions shaped by natural selection. Expanding the concept of homeostasis to include intermittent and stochastic forms will improve our understanding of physiological adaptation, with significant implications for ecology and evolutionary biology. We further advocate new experimental approaches to distinguish adaptive variability from true regulatory failure.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 20","pages":"e71150"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TLR4 signaling cascades leading to increases in albumin permeability, nephrin ectodomain shedding, and apoptosis in MPC-5 podocytes. TLR4信号级联导致MPC-5足细胞白蛋白通透性增加、肾素外结构域脱落和凋亡。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71147

Eun Young Kim, Stuart E Dryer

{"title":"TLR4 signaling cascades leading to increases in albumin permeability, nephrin ectodomain shedding, and apoptosis in MPC-5 podocytes.","authors":"Eun Young Kim, Stuart E Dryer","doi":"10.14814/phy2.71147","DOIUrl":"10.14814/phy2.71147","url":null,"abstract":"Proteinuria is a cardinal symptom of kidney disease, but common transient proteinurias may reflect a normal physiological response to distant tissue damage or infection. Here we observed that activation of Toll-like receptor-4 (TLR4) using the selective agonist CRX572 induced three distinct functional effects in cultured podocytes. TLR4 activation evoked a reversible increase in albumin flux (PAlb) across a confluent layer of podocytes in 60 m or less. At 24 h matrix metalloproteinase-dependent shedding of nephrin ectodomains was detected. Finally, apoptosis occurred after 72 h. These effects were blocked by TAK242, a selective TLR4 antagonist. TLR4 activation engaged multiple intracellular transduction cascades. Activation of Erk was observed at 30 m, increased reactive oxygen species (ROS) was seen at 60 m and 24 h, and activation of NFκB was seen with 24 h of TLR activation. The increase in PAlb required increased ROS and activation of Erk. The subsequent nephrin ectodomain shedding also required Erk signaling but did not require increased cytosolic ROS or NFκB activation. Finally, TLR4-evoked apoptosis required both increased ROS and activation of NFκB but did not require Erk activation. Innate immune signaling through TLR4 may alter glomerular function via parallel pleiotropic signaling cascades in podocytes.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 20","pages":"e71147"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13673513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149196108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Original W'-balance models do not transfer to high-intensity interval training in trained adult runners: Proposal of a single-coefficient linear alternative. 原始的W'平衡模型不能转移到训练有素的成年跑步者的高强度间歇训练：单系数线性替代方案的建议。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71152

Felipe García-Pinillos, Diego Jaén-Carrillo, Leonardo Riquelme-Sebastián, Santiago Alejo Ruiz-Alias, Iván Fernández-Navarrete, Fernando González-Mohino, Violeta Muñoz de la Cruz, Javier Olaya-Cuartero

{"title":"Original W'-balance models do not transfer to high-intensity interval training in trained adult runners: Proposal of a single-coefficient linear alternative.","authors":"Felipe García-Pinillos, Diego Jaén-Carrillo, Leonardo Riquelme-Sebastián, Santiago Alejo Ruiz-Alias, Iván Fernández-Navarrete, Fernando González-Mohino, Violeta Muñoz de la Cruz, Javier Olaya-Cuartero","doi":"10.14814/phy2.71152","DOIUrl":"https://doi.org/10.14814/phy2.71152","url":null,"abstract":"This study evaluated the off-the-shelf predictive accuracy of four original W'-balance recovery models (Morton and Billat linear, W'BAL-INT, W'BAL-ODE and W'BAL-CAEN) and proposed a single-coefficient modified linear formulation (Linear_mod) for predicting high-intensity interval training (HIIT) performance with passive recovery in trained runners. Twenty-seven trained runners completed a 9/3-min running critical power (CP) test and two open-ended HIIT sessions to volitional exhaustion at 108% CP with a 2:1 work: rest ratio. Work intervals were individualized so that energy expenditure above CP equalled 40% (HIIT40%) or 60% (HIIT60%) of W'. The original models were applied using their published parameters. All substantially over-predicted the number of intervals completed (pooled MAE 26.4-72.4 intervals; bias +20.1 to +72.4). Linear_mod with its default recovery coefficient (R = 1) achieved a MAE of 4.85 intervals. Optimizing R at the population level (R = 1.63) reduced MAE to 2.85 intervals, while athlete-specific optimisation (R = 1.54 ± 0.29) reduced MAE to 0.55 intervals. Individual R values were stable between protocols (within-subject CV = 9.9%). Original W'-balance models did not accurately predict running HIIT performance, whereas Linear_mod provided high predictive accuracy using a single athlete-specific recovery coefficient.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 20","pages":"e71152"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149228576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heliox versus trimix in closed-circuit rebreather diving: A comparative study of pulmonary and decompression stress in male divers. 闭合循环再呼吸潜水中的Heliox与trimix：男性潜水员肺和减压压力的比较研究。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71129

Emmanuel Gouin, Jérémy Orsat, Emmanuel Dugrenot, Rhiannon J Brenner, Géraldine Leman, Eléonore Bettacchioli, Michael Theron, Marie-Agnès Giroux-Metges, Erwan L'Her, François Guerrero

{"title":"Heliox versus trimix in closed-circuit rebreather diving: A comparative study of pulmonary and decompression stress in male divers.","authors":"Emmanuel Gouin, Jérémy Orsat, Emmanuel Dugrenot, Rhiannon J Brenner, Géraldine Leman, Eléonore Bettacchioli, Michael Theron, Marie-Agnès Giroux-Metges, Erwan L'Her, François Guerrero","doi":"10.14814/phy2.71129","DOIUrl":"10.14814/phy2.71129","url":null,"abstract":"Closed-circuit rebreather (CCR) and helium-based breathing mixtures are widely used in technical diving. This study compared the effects of Heliox and Trimix on pulmonary function and decompression stress during CCR dives. Nineteen divers performed a standardized dive (50 ± 3 msw, 147 ± 10 min) using Heliox (16% O2/84% He; n = 9) or Trimix (16% O2/32% He/52% N2; n = 10). Pre- and post-dive venous gas emboli (VGE), pulmonary function, lung ultrasound, heart rate variability, and inflammatory parameters were assessed. VGE were detected in all Heliox divers but in only 50% of Trimix divers. Heliox exhibited higher VGE counts 45 min post-dive than Trimix (12 [IQR 3-32] vs. 0 [IQR 0-6], p = 0.03); this difference disappeared by 90 min. No clinically significant pulmonary function changes were observed. Lung diffusion capacity decreased moderately regardless of breathing gas (-6% ± 7%). Lung ultrasound revealed minor transient B-line changes. Dive induced mild inflammatory responses, including neutrophilia and increased MRP8/14 in both groups. Heliox divers also showed transient complement activation alongside increased circulating platelets. CCR dives induced limited pulmonary stress, without gas-dependent differences. Although Heliox may promote a higher post-dive VGE burden and inflammatory responses, the relationship between these physiological effects and decompression sickness risk remains uncertain.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 19","pages":"e71129"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13639384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149113046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0