Physiological Reports最新文献_第2页

Contributions of the anion exchangers SLC26A1, SLC26A2, SLC26A3, and SLC26A6 to intestinal oxalate transport in human Caco2-BBE cells. 阴离子交换剂SLC26A1、SLC26A2、SLC26A3和SLC26A6对人Caco2-BBE细胞肠道草酸盐转运的贡献

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71125

Khalid A Abdelhalim, Donna Arvans, Yanzhe Wang, Mohamed Elfatih H Bashir, Hatim A Hassan

{"title":"Contributions of the anion exchangers SLC26A1, SLC26A2, SLC26A3, and SLC26A6 to intestinal oxalate transport in human Caco2-BBE cells.","authors":"Khalid A Abdelhalim, Donna Arvans, Yanzhe Wang, Mohamed Elfatih H Bashir, Hatim A Hassan","doi":"10.14814/phy2.71125","DOIUrl":"10.14814/phy2.71125","url":null,"abstract":"Kidney stones affect ~1 in 5 men and 1 in 11 women, with 70%-80% being composed of calcium oxalate. Knowing the contributions of anion exchangers SLC26A2 (A2), A3, and A6 to apical and A1 to basolateral oxalate transport in human intestinal Caco2-BBE (C2) cells is important since enhancing intestinal oxalate secretion (A6/2/?A1) and reducing intestinal oxalate absorption (A3) are potential therapeutic targets. We previously showed A6 contributes > A2 to oxalate transport in C2 cells. Here, we report the contributions of combined A2+A6, A1, and A3 to oxalate transport in C2 cells using siRNA knockdown. A2 (74% reduced mRNA expression), A6 (64% reduced protein abundance), and A2+A6 (combined knockdown utilizing same A2 or A6 C2 silencing batches) mediate 31.1%, 57%, and 78% of apical oxalate transport, respectively. A1 mediates 30.9% (59.5% reduced protein abundance) of basolateral oxalate transport. A3 (78.7% reduced protein abundance) has no significant contribution to apical oxalate transport. In conclusion, most apical oxalate transport in C2 cells is mediated by A2+A6 (with no significant contribution from A3) while A1 contributes to basolateral oxalate transport. Thus, C2 cells can be used for screening/testing novel drugs targeting these transporters, which will have significant potential therapeutic implications for oxalate-related disorders.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 19","pages":"e71125"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13652957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149149581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced but not superior: Performance outcomes from the inaugural enhanced games. 增强但不卓越：首届增强游戏的性能结果。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71123

Rafael A Azevedo, Luis F Coutinho, Monique C Gonçalves, Higor A Oliveira, Guilherme J Gonçalves, Marcus Campos, Hamilton Roschel, Bruno Gualano

{"title":"Enhanced but not superior: Performance outcomes from the inaugural enhanced games.","authors":"Rafael A Azevedo, Luis F Coutinho, Monique C Gonçalves, Higor A Oliveira, Guilherme J Gonçalves, Marcus Campos, Hamilton Roschel, Bruno Gualano","doi":"10.14814/phy2.71123","DOIUrl":"10.14814/phy2.71123","url":null,"abstract":"The Enhanced Games (TEG) emerged as an athletic event engineered to profit from biotechnological enhancement, such as performance-enhancing drugs (PEDs) utilization, relying on three premises: shattering world records (WR), enabling athletes to surpass their personal bests (PB), and utilizing PEDs to offset age-related physical decline. The effectiveness of TEG's PED protocols in achieving these three premises was evaluated. This is a retrospective analysis from 42 TEG athletes across Track, Swimming, and Weightlifting. TEG's performances were compared to current WR and athletes' PB previous to TEG. The anti-aging PED effects were evaluated by the relationship between the age and performance gaps from PB to TEG competition. Athletes' previous PBs were -5.92% ± 4.68% worse than current WR, which further deteriorated to -8.91% ± 7.52% after TEG, and a single WR was broken. Whereas 19 new PBs were recorded, TEG performances were on average -2.86% ± 7.39% worse than previous PB. Some competitive results were achieved by non-enhanced athletes. Performance decline continued linearly as the time elapsed since the athletes' physical prime (i.e., age at PB). TEG failed to deliver on core promises of widespread WR and broad athletic enhancement, and PED protocol did not attenuate expected age-related performance declines.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 19","pages":"e71123"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13623661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue-specific insulin sensitivity in anorexia nervosa: A cross-sectional positron emission tomography (PET) study in female adults. 神经性厌食症的组织特异性胰岛素敏感性：一项成年女性正电子发射断层扫描（PET）研究。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71142

Aino Latva-Rasku, Kyoungjune Pak, Eleni Rebelos, Miikka-Juhani Honka, Prince Dadson, Jukka Koffert, Jouni Tuisku, Laura Pekkarinen, Sanna Laurila, Janina Hieta, Kirsi Laitinen, Anna K Kirjavainen, Jussi Hirvonen, Henry K Karlsson, Max Karukivi, Lauri Nummenmaa, Pirjo Nuutila

{"title":"Tissue-specific insulin sensitivity in anorexia nervosa: A cross-sectional positron emission tomography (PET) study in female adults.","authors":"Aino Latva-Rasku, Kyoungjune Pak, Eleni Rebelos, Miikka-Juhani Honka, Prince Dadson, Jukka Koffert, Jouni Tuisku, Laura Pekkarinen, Sanna Laurila, Janina Hieta, Kirsi Laitinen, Anna K Kirjavainen, Jussi Hirvonen, Henry K Karlsson, Max Karukivi, Lauri Nummenmaa, Pirjo Nuutila","doi":"10.14814/phy2.71142","DOIUrl":"10.14814/phy2.71142","url":null,"abstract":"Anorexia nervosa (AN) has been linked to both impaired and improved whole-body insulin sensitivity, but tissue-specific metabolic alterations remain unexplored. To address this gap, we studied 12 female volunteers with recently diagnosed AN and 13 healthy, lean female controls in a cross-sectional design using positron emission tomography (PET) imaging with the glucose analogue [18F]FDG tracer under hyperinsulinemic conditions. We show that tissue-specific glucose uptake during hyperinsulinemia was higher only in the duodenum of individuals with AN, but comparable in other tissues, possibly indicating altered intestinal glucose handling and nutrient sensing in reduced energy intake. In five participants with AN, glucose uptake was elevated despite reduced skeletal muscle glucose utilization. In controls, growth hormone (GH) levels correlated with skeletal muscle, liver, and brain insulin sensitivity, but these associations were absent in AN, suggesting disrupted endocrine regulation of glucose metabolism. Overall, whole-body insulin sensitivity was not altered, yet tissue-specific glucose metabolism exhibited greater variability in AN. These differences were not directly explained by disease duration or weight loss severity, suggesting contributions from individual genetic background and behavioral patterns.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 19","pages":"e71142"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13653040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149148434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Test-retest reliability and minimal detectable change of pulse wave velocity and flow-mediated dilation in paretic and non-paretic limbs of older adults post-stroke. 脑卒中后老年人卒中后瘫瘫和非瘫瘫肢体的脉搏波速度和血流介导的舒张的最小可检测变化。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71138

Juliano A Abreu, Natalie D'Isabella, Derek W Stouth, Maureen J MacDonald, Ada Tang, Kevin Moncion

{"title":"Test-retest reliability and minimal detectable change of pulse wave velocity and flow-mediated dilation in paretic and non-paretic limbs of older adults post-stroke.","authors":"Juliano A Abreu, Natalie D'Isabella, Derek W Stouth, Maureen J MacDonald, Ada Tang, Kevin Moncion","doi":"10.14814/phy2.71138","DOIUrl":"https://doi.org/10.14814/phy2.71138","url":null,"abstract":"Arterial stiffness and endothelial dysfunction are non-traditional risk markers after stroke, yet reliability and minimal detectable change (MDC) of pulse wave velocity (PWV) and flow-mediated dilation (FMD) have not been established. We examined test-retest reliability and MDC of carotid-femoral (cfPWV), carotid-radial (crPWV), and femoral-dorsalis pedis PWV (ffPWV), and absolute and relative brachial artery FMD in people >12 months post-stroke. Seventeen individuals (n = 4 (24%) females; 63.9 ± 8.6 years; 4.5 ± 2.8 years post-stroke) completed two bilateral assessments of PWV via applanation tonometry and FMD via duplex ultrasound within 1 week. Intraclass correlation coefficients (ICC2,1) were calculated via two-way random-effects models, and MDC derived at 68%, 90%, and 95% confidence levels. We found good-to-excellent reliability for cfPWV in paretic (ICC2,1 = 0.85; 95% CI:0.59, 0.95) and non-paretic (ICC2,1 = 0.94; 95% CI:0.82, 0.98) limbs, with MDC95% values of 2.23 and 1.68 m/s, respectively. crPWV and ffPWV exhibited poor reliability bilaterally (ICC2,1 = 0.12-0.33). Relative FMD showed moderate reliability for paretic (ICC2,1 = 0.50; 95% CI: -0.20, 0.85) and non-paretic (ICC = 0.59; 95% CI:0.04, 0.84) limbs, with MDC95% values of 6.88% and 7.65%, respectively. Reliability for absolute FMD was poor (ICC2,1 = 0.11-0.44). These findings support cfPWV as a reliable vascular assessment in individuals >12 months post-stroke, and provide stroke-specific MDC values to guide interpretability in future trials.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 20","pages":"e71138"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skeletal muscle fatigue resistance is differentially affected in 8-month-old male and female hAβ-KI mice. 8月龄雄性和雌性hAβ-KI小鼠骨骼肌抗疲劳能力受到不同程度的影响。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71153

Emily N Copeland, Evelin Melekh, Val Andrew Fajardo, Rebecca E K MacPherson

{"title":"Skeletal muscle fatigue resistance is differentially affected in 8-month-old male and female hAβ-KI mice.","authors":"Emily N Copeland, Evelin Melekh, Val Andrew Fajardo, Rebecca E K MacPherson","doi":"10.14814/phy2.71153","DOIUrl":"https://doi.org/10.14814/phy2.71153","url":null,"abstract":"Approximately 95% of Alzheimer's disease (AD) diagnoses are sporadic onset, present after the age of 65, and arising from a combination of non-modifiable and modifiable factors. This underscores the potential to identify early systemic changes that may contribute to disease development. One factor may be skeletal muscle atrophy, which has been associated with cognitive decline, although the exact relationship between skeletal muscle and the brain is unknown. Furthermore, whether muscle atrophy begins prior to or after the onset of AD remains elusive. The humanized amyloid-beta knock-in mouse (hAβ-KI) exhibits age-related cognitive decline, insoluble Aβ accumulation, inflammation and microglial activation, making it a useful model of sporadic AD. However, characterization of skeletal muscle changes with aging has yet to be examined. Here, we assess skeletal muscle form and function of the hAβ-KI mouse at 8 and 15 months of age, before and after onset of cognitive decline, through assessment of whole-body lean mass percentage, isolated muscle contraction of the extensor digitorum longus (EDL) and soleus muscle, and fiber type analysis of male and female mice. Whole-body lean mass declined over time in all mice; however, only male hAβ-KI mice had a significant change in body mass and lean mass over the study. At 8-months-old, the isolated EDL of male hAβ-KI mice fatigued faster than the WT mice, but there were no differences at 15 months-old nor fiber type composition at either age. Alternatively, the isolated EDL and soleus of 8-month-old hAβ-KI female mice fatigued slower compared to the WT mice, and there were no differences at 15 months nor fiber type composition at either age. This preliminary characterization work suggests sex-dependent differences in isolated muscle fatigue prior to cognitive decline in the hAβ-KI mouse model of sporadic AD.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 20","pages":"e71153"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149228607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altitude and child development: A cross-sectional comparative study of children in urbanized low-altitude and rural high-altitude settings in Kyrgyzstan. 海拔与儿童发展：吉尔吉斯斯坦城市化低海拔地区和农村高海拔地区儿童的横断面比较研究。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71132

Adam Bøcker Toft Olsen, Elvira Isaeva, Madina Umetalieva, Nargiza Osmonbaeva, Sofie Valborg Wright, Lea Deniz Lund Serefoglu, Freya Natasha Kretzchmar, Trisse Dyrløv Høegh, Jesper Kjærgaard, Kim Gjerum Nielsen, Talant Sooronbaev, Anja Poulsen

{"title":"Altitude and child development: A cross-sectional comparative study of children in urbanized low-altitude and rural high-altitude settings in Kyrgyzstan.","authors":"Adam Bøcker Toft Olsen, Elvira Isaeva, Madina Umetalieva, Nargiza Osmonbaeva, Sofie Valborg Wright, Lea Deniz Lund Serefoglu, Freya Natasha Kretzchmar, Trisse Dyrløv Høegh, Jesper Kjærgaard, Kim Gjerum Nielsen, Talant Sooronbaev, Anja Poulsen","doi":"10.14814/phy2.71132","DOIUrl":"10.14814/phy2.71132","url":null,"abstract":"Childhood malnutrition remains a global challenge; Kyrgyzstan has reduced it, though disparities persist. We hypothesized that developmental differences between lowland and highland children reflect altitude adaptation, not malnutrition alone, obscuring monitoring. This study compared children from Naryn (2400 m) to Bishkek (800 m), examining respiratory, hematological, and anthropometric development. We conducted a cross-sectional study among healthy Kyrgyz schoolchildren aged 10-12 from Naryn and Bishkek. Measurements included anthropometrics, spirometry, hemoglobin, hand-grip strength, and vital signs. Linear regression controlled for socioeconomic, maternal, nutritional, and healthcare factors. Compared to Naryn (n = 143), Bishkek children (n = 123) were taller (2.7 cm, p = 0.006), heavier (2.7 kg, p = 0.008), with larger waist (2.7 cm, p = 0.007) and mid-upper arm circumferences (0.7 cm, p = 0.023), lower hemoglobin (-14.4 g/L, p < 0.001), higher oxygen saturation (2.1%, p < 0.001), weaker hand-grip strength (-1.3 kgf, p = 0.013), and higher HAZ (0.5, p < 0.001). No differences existed in BMI or BAZ. Naryn males were shorter than females (-3.60 cm, p = 0.024). Bishkek children had lower FEV1 (-0.4, p = 0.006) and FVC z-scores (-0.48, p = 0.003). High-altitude Kyrgyz children showed developmental and respiratory patterns suggesting physiological adaptation, though malnutrition cannot be excluded. Lower birthweight partially explained reduced height but not lung function or male-specific growth, highlighting the need for altitude-specific growth and respiratory references and continued research in pediatric high-altitude populations.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 19","pages":"e71132"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13658936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149168077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is cardiovascular autonomic control during the day-to-night transition dependent on dim light melatonin onset? An exploratory analysis in healthy adults. 在昼夜转换期间，心血管自主控制是否依赖于昏暗光线下褪黑激素的发作？健康成人的探索性分析。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71145

Leandro C Brito, Megan Jones, Caroline P Garcês, Steven A Shea, Saurabh S Thosar

{"title":"Is cardiovascular autonomic control during the day-to-night transition dependent on dim light melatonin onset? An exploratory analysis in healthy adults.","authors":"Leandro C Brito, Megan Jones, Caroline P Garcês, Steven A Shea, Saurabh S Thosar","doi":"10.14814/phy2.71145","DOIUrl":"10.14814/phy2.71145","url":null,"abstract":"We tested whether dim-light melatonin onset (DLMO) is time-aligned with the transition between biological day into night in cardiovascular autonomic control (CV onset time). We studied 10 healthy adults (4 females, age 25 ± SD 2 years) under an ultra-short semi-constant routine protocol (<8lux). Participants remained seated for 7 h until 1 h after their habitual bedtime. We collected saliva samples every 30 min and recorded R-R intervals and beat-to-beat blood pressure every hour. DLMO was defined as the interpolated time for melatonin increase over 3 pg/mL. Phase angle was defined as the time difference between DLMO and the midpoint of self-reported sleep as a measure of the alignment between sleep-wake patterns and biological circadian rhythms. CV onset time was defined using a segmental (piecewise) analysis to identify the inflection point (X0 slope) throughout the study. CV autonomic modulation was assessed using indices of heart rate and blood pressure variability. CV onset times for all indices did not differ significantly from DLMO (p > 0.05). Mean differences between DLMO and CV onset time were significantly correlated with the phase angle of entrainment (all significant, r > +0.75). Our results suggest that CV autonomic transition into biological night was not associated with DLMO, which seems to depend on the phase angle of entrainment.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 20","pages":"e71145"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13675520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149207461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of TMS stimulus direction on corticospinal and cortico-cortical excitability of the lower limb in healthy adults. 经颅磁刺激方向对健康成人下肢皮质-脊髓及皮质-皮质兴奋性的影响。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71144

M C Hall, M Zoghi, L Perraton, S Jaberzadeh

{"title":"The effects of TMS stimulus direction on corticospinal and cortico-cortical excitability of the lower limb in healthy adults.","authors":"M C Hall, M Zoghi, L Perraton, S Jaberzadeh","doi":"10.14814/phy2.71144","DOIUrl":"10.14814/phy2.71144","url":null,"abstract":"Transcranial magnetic stimulation (TMS) studies in upper limb muscles have shown that stimulus direction can influence corticospinal and intracortical excitability; however, limited evidence exists for lower limb muscles. This study investigated the effect of TMS stimulus direction on corticospinal and intracortical excitability of the tibialis anterior (TA) muscle using paired-pulse TMS. Nineteen healthy adults (10 males, 9 females; mean age 41.8 ± 23 years) participated. Single- and paired-pulse TMS was delivered using a figure-of-eight coil oriented to induce anterior-to-posterior (AP) or posterior-to-anterior (PA) current flow in the left motor cortex while recording motor evoked potentials (MEPs) from the right TA. Resting motor threshold (RMT), short-interval intracortical inhibition (SICI), long-interval intracortical inhibition (LICI), and intracortical facilitation (ICF) were assessed under both stimulus directions in a single session. No significant differences were identified between AP and PA stimulation for RMT, SICI, or LICI (p > 0.05). However, AP stimulation produced significantly greater ICF responses compared with PA stimulation (p = 0.027). AP and PA stimulus directions produced comparable RMT, SICI, and LICI measures in the TA muscle, although AP stimulation elicited greater ICF. These findings suggest facilitatory intracortical circuits may be more sensitive to stimulus direction than inhibitory pathways.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 20","pages":"e71144"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13673296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149196095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supraphysiological ascorbic acid infusion attenuates cerebrovascular dysfunction in middle-aged and older adults. 生理上抗坏血酸输注减轻中老年人脑血管功能障碍。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71120

Theodore M DeConne, Faria Sanjana, Elizabeth M Habash, Kevin P Decker, Wendy Nichols, Joshua C Hobson, Nicholas A Rizzi, Christopher R Martens

{"title":"Supraphysiological ascorbic acid infusion attenuates cerebrovascular dysfunction in middle-aged and older adults.","authors":"Theodore M DeConne, Faria Sanjana, Elizabeth M Habash, Kevin P Decker, Wendy Nichols, Joshua C Hobson, Nicholas A Rizzi, Christopher R Martens","doi":"10.14814/phy2.71120","DOIUrl":"https://doi.org/10.14814/phy2.71120","url":null,"abstract":"Cerebrovascular endothelial function can be assessed by measuring the shear-mediated dilation or cerebrovascular reactivity (CVR) of the internal carotid artery (ICA) in response to transient hypercapnia (30 s). ICA CVR is blunted with aging; however, it is not known whether age-related reductions in ICA CVR are influenced by antioxidant-responsive mechanisms. The purpose of this study was to determine whether infusion of the antioxidant, ascorbic acid (AA) restores ICA CVR in middle-aged and older (MA/O) adults. We also examined the effect of AA on middle cerebral artery (MCA) CVR to sustained (3-min) hypercapnia. We hypothesized that MA/O adults would exhibit lower cerebrovascular function compared to young (Y) adults, and that infusion of AA would improve cerebrovascular function in MA/O adults, whereas infusion of saline (SAL) would not. The primary analytic sample included nine Y adults (25 ± 2 years) and nineteen (65 ± 6 years) MA/O adults, with analyzable data varying by outcome. ICA CVR was measured using high-resolution duplex ultrasound, and MCA CVR was measured using transcranial Doppler ultrasound. AA infusion improved ICA CVR and MCA CVR in MA/O adults but had no effect in Y adults. These findings suggest that age-related impairments in cerebrovascular function may be partly attributable to mechanisms responsive to acute, supraphysiological AA administration.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 19","pages":"e71120"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13635807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149113466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors influencing baseline electrocardiographic parameters in rats: A systematic review of experimental studies published from 1960 to the present. 影响大鼠基线心电图参数的因素：对1960年至今发表的实验研究的系统回顾。

IF 2.1

Physiological Reports Pub Date : 2026-10-01 DOI: 10.14814/phy2.71131

Viktória Novotná, Soňa Grešová, Pavol Švorc

{"title":"Factors influencing baseline electrocardiographic parameters in rats: A systematic review of experimental studies published from 1960 to the present.","authors":"Viktória Novotná, Soňa Grešová, Pavol Švorc","doi":"10.14814/phy2.71131","DOIUrl":"10.14814/phy2.71131","url":null,"abstract":"Experimental studies in rats use baseline or control values as a reference against which changes after experimental interventions are assessed. However, baseline or control ECG parameters may already be influenced by several factors before the experiment begins, complicating interpretation of the results. Therefore, this review summarizes baseline or control ECG parameters (heart rate, PR (or PQ) interval, QRS complex, QT and QTc intervals, P-wave duration and amplitude, R- and T-wave amplitudes, and ST-segment) from 285 in vivo rat studies published from 1960 to the present, identified through searches of Web of Science, Scopus, and PubMed. The aim of this review was not to assess the quality of the included studies, but to help researchers navigate the data and identify potential biological and methodological sources of variability or confounding affecting baseline or control ECG parameters. Our findings should be interpreted with caution due to limitations including variability and small datasets in some groups, unequal sample sizes, aggregation of study-level mean values into a single mean, grouping of different anesthetic agents into a category, underrepresentation of females, incomplete reporting of anesthesia and measurement time, and inconsistent QTc correction methods. These limitations highlight the need for standardized experimental protocols and reporting practices.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"14 19","pages":"e71131"},"PeriodicalIF":2.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149167031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0