Physiological Reports最新文献_第10页

Roux-en-Y gastric bypass surgery decreases intestinal aryl hydrocarbon receptor signaling in male mice. Roux-en-Y胃旁路手术降低雄性小鼠肠道芳烃受体信号。

IF 2.2

Physiological Reports Pub Date : 2025-08-01 DOI: 10.14814/phy2.70524

Annie Wang, Madelynn E Agabao-Tucker, Michael L Goodson, Allison K Ehrlich, Bethany P Cummings

{"title":"Roux-en-Y gastric bypass surgery decreases intestinal aryl hydrocarbon receptor signaling in male mice.","authors":"Annie Wang, Madelynn E Agabao-Tucker, Michael L Goodson, Allison K Ehrlich, Bethany P Cummings","doi":"10.14814/phy2.70524","DOIUrl":"https://doi.org/10.14814/phy2.70524","url":null,"abstract":"The impact of Roux-en-Y gastric bypass (RYGB) on indole metabolism and AhR signaling is poorly understood. Therefore, we tested the hypothesis that RYGB changes the indole metabolite profile in the gut, leading to changes in AhR signaling. To test this hypothesis, we developed a mouse model of RYGB that recapitulates the human procedure. Given that indole metabolism is influenced by body weight, we included sham-operated control mice that were either fed ad libitum (S-AL) or food restricted to match body weight to RYGB (S-WM). We measured weight- and surgery-dependent changes to glucose metabolism, indole metabolism, and AhR activation. RYGB-operated mice exhibited body weight-independent improvements in glucose tolerance, insulin, and glucagon-like peptide-1 secretion. Plasma levels of indole-3-propionic acid (I3PA) and kynurenic acid (KA) were reduced in RYGB mice compared to S-WM mice, but not S-AL mice. However, the S-WM group exhibited a trend for increased I3PA and KA compared to S-AL. Consistent with the reduction in indoles, RYGB reduced AhR signaling and increased lipocalin-2 expression, a marker of inflammation. These data suggest that indole metabolites do not contribute to the metabolic benefits of RYGB, but their reduction may contribute to increases in gut inflammation after RYGB.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 16","pages":"e70524"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12378703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Convergent skeletal muscle cytokine responses to TFEB overexpression and voluntary wheel running reflect sex-based variability to exercise adaptations in mice. 骨骼肌细胞因子对TFEB过表达的趋同反应和自愿轮式跑步反映了小鼠运动适应的性别差异。

IF 2.2

Physiological Reports Pub Date : 2025-08-01 DOI: 10.14814/phy2.70519

Dalton C Patterson, Allison Birnbaum, Ian Matthews, Constanza J Cortes

{"title":"Convergent skeletal muscle cytokine responses to TFEB overexpression and voluntary wheel running reflect sex-based variability to exercise adaptations in mice.","authors":"Dalton C Patterson, Allison Birnbaum, Ian Matthews, Constanza J Cortes","doi":"10.14814/phy2.70519","DOIUrl":"https://doi.org/10.14814/phy2.70519","url":null,"abstract":"Running promotes skeletal muscle remodeling through metabolic and inflammatory signaling pathways, though the extent to which these responses are sex-dependent remains unclear. We profiled cytokine responses in quadriceps lysates from sedentary, voluntary wheel-running (VWR), and muscle-specific TFEB-overexpressing (cTFEB;HSACre) male and female mice. Cytokine analysis revealed 40 differentially expressed factors associated with exercise and/or TFEB overexpression, many exhibiting sex-dimorphic patterns. In males, VWR increased interleukins (IL-1α, IL-1β, IL-2, IL-5, and IL-17) and chemokines (e.g., MCP-1, CCL5, and CXCL9), including components of TNF signaling (e.g., TNFα, sTNFR1/2, and Fas ligand). These elevations were partially recapitulated by TFEB overexpression in sedentary males. In contrast, female VWR muscle showed limited changes, with significant differences restricted to IL-3, IL-3Rb, IL-13, and CXCL16. These findings demonstrate sex-specific cytokine responses to endurance-like stimuli and suggest a broader or more prolonged inflammatory remodeling profile in male skeletal muscle. Moreover, muscle-specific TFEB overexpression reproduced similar endurance-induced cytokine changes, particularly in males, highlighting TFEB as a partial molecular mimic of exercise-associated inflammatory signaling in skeletal muscle. Together, our data underscore the importance of sex as a biological variable in exercise-induced cytokine remodeling and support the utility of TFEB overexpression as a platform for prioritizing exercise-associated phenotypes.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 16","pages":"e70519"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of sugammadex on heart rate variability in anesthetized male rats. 糖madex对麻醉雄性大鼠心率变异性的影响。

IF 2.2

Physiological Reports Pub Date : 2025-08-01 DOI: 10.14814/phy2.70507

Mika Nishikawa, Toru Kawada, Keita Saku, Hiroyuki Kinoshita, Shinji Kawahito

引用次数: 0

Comparative analysis of morning and evening training on performance and well-being in elite soccer players. 优秀足球运动员早晚训练对成绩和幸福感的影响比较分析。

IF 2.2

Physiological Reports Pub Date : 2025-08-01 DOI: 10.14814/phy2.70510

Okba Selmi, Mohamed Amine Rahmoune, Anissa Bouassida, Santo Marsigliante, Antonella Muscella

{"title":"Comparative analysis of morning and evening training on performance and well-being in elite soccer players.","authors":"Okba Selmi, Mohamed Amine Rahmoune, Anissa Bouassida, Santo Marsigliante, Antonella Muscella","doi":"10.14814/phy2.70510","DOIUrl":"10.14814/phy2.70510","url":null,"abstract":"The purpose of the study was to determine how training at different times of day affects soccer players' performance, mood, and physiological responses. Twenty male professional soccer players participated in the study, which involved small-sided games (SSGs) conducted in both morning and evening sessions. Players' mood states, perceived exertion, heart rate, and performance metrics were measured to assess the impact of circadian rhythms on their physical and psychological responses. Results showed significantly higher HRmean (p = 0.025) and %HRmax (p = 0.022) and significantly lower blood lactate concentration (p = 0.015) in the morning. Despite similar perceived exertion and recovery scores, players reported greater enjoyment in evening sessions (p = 0.0008). Mood states were more favorable in the evening, with significantly lower levels of stress (p = 0.007), fatigue (p = 0.009), and muscle soreness (p = 0.012). The Hooper's Index was also markedly lower in the evening (p = 0.0008). Evening performance was superior, with significantly more ball interceptions (p = 0.033), successful passes (p = 0.0004), and ball possession recoveries (p = 0.025), alongside fewer lost balls in the evening compared to the morning (p = 0.008). In conclusion, the study demonstrates that time of day significantly influences both psychological and physical responses in professional soccer players, with evening sessions leading to a more favorable mood and better performance. These findings support the consideration of circadian rhythms when scheduling training and matches to optimize athletic outcomes.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 15","pages":"e70510"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12339409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144822231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipocyte-specific Krüppel-like factor 14 overexpression confers sex-biased protection from weight gain on a high-fat diet. 脂肪细胞特异性kr<s:1> ppel样因子14的过度表达赋予性别偏见保护免受高脂肪饮食的体重增加。

IF 2.2

Physiological Reports Pub Date : 2025-08-01 DOI: 10.14814/phy2.70513

Yonathan Tamrat Aberra, Qianyi Yang, Ashlyn Cowan, Bilge Yaylak Gediksiz, Mitchell Thomas, David Gragirenes-Delgado, Foday Keita, Mete Civelek

{"title":"Adipocyte-specific Krüppel-like factor 14 overexpression confers sex-biased protection from weight gain on a high-fat diet.","authors":"Yonathan Tamrat Aberra, Qianyi Yang, Ashlyn Cowan, Bilge Yaylak Gediksiz, Mitchell Thomas, David Gragirenes-Delgado, Foday Keita, Mete Civelek","doi":"10.14814/phy2.70513","DOIUrl":"10.14814/phy2.70513","url":null,"abstract":"Metabolic syndrome, a constellation of cardiometabolic risk factors including abdominal obesity, predisposes individuals to type 2 diabetes and cardiovascular disease. One frequently replicated MetSyn genetic association signal is located near KLF14 and is linked to central adiposity, with stronger effects observed in females. Lower KLF14 expression has been associated with detrimental metabolic phenotypes; however, the therapeutic effect of KLF14 germline overexpression remains unexplored. Here, we generated an adipocyte-specific Klf14 overexpression mouse model to investigate its role in metabolic regulation. Transgenic overexpression conferred sex-dependent metabolic benefits, including reduced weight gain, improved body composition, and enhanced acute insulin sensitivity, predominantly in female mice. These effects were accompanied by increased expression of genes involved in lipid uptake and thermogenic browning of white adipose tissue. Our findings demonstrate that KLF14 overexpression confers protective metabolic effects in a sex-specific manner and support the potential of KLF14-targeted strategies for treating metabolic syndrome.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 15","pages":"e70513"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12339416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144822230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic shifts in plasma amino acids and related metabolites in response to SGLT2 inhibition and hyperglycemia in type 1 diabetes. 1型糖尿病患者血浆氨基酸和相关代谢物对SGLT2抑制和高血糖的代谢变化

IF 2.2

Physiological Reports Pub Date : 2025-08-01 DOI: 10.14814/phy2.70465

Luxcia Kugathasan, Nagarjunachary Ragi, Subrata Debnath, Vikas S Sridhar, Soumya Maity, Tianqing Feng, Esmeralda Treviño, Bruce A Perkins, David Z I Cherney, Kumar Sharma

{"title":"Metabolic shifts in plasma amino acids and related metabolites in response to SGLT2 inhibition and hyperglycemia in type 1 diabetes.","authors":"Luxcia Kugathasan, Nagarjunachary Ragi, Subrata Debnath, Vikas S Sridhar, Soumya Maity, Tianqing Feng, Esmeralda Treviño, Bruce A Perkins, David Z I Cherney, Kumar Sharma","doi":"10.14814/phy2.70465","DOIUrl":"https://doi.org/10.14814/phy2.70465","url":null,"abstract":"Regulated kidney function is dependent on maintaining efficient energy utilization. Our aim in this study was to determine the effects of acute, ambient hyperglycemia and sodium-glucose cotransporter-2 (SGLT2) inhibition on plasma amino acid metabolism in patients with type 1 diabetes (T1D). The ATIRMA trial, a single-arm study, evaluated the effects of 8 weeks of oral empagliflozin (25 mg/day) in 40 young adults with T1D. The study involved consecutive two-day assessments of clamped euglycemia and hyperglycemia at both baseline and post-treatment. MetaboAnalyst 6.0 categorized 35 metabolites into significant pathways, which were statistically compared using principal component analysis. Acute hyperglycemia induced changes to 10 metabolic pathways, including but not limited to increases in cysteine and methionine metabolism (0.52 ± 0.12, p < 0.0001), valine, leucine, and isoleucine biosynthesis (0.31 ± 0.10, p = 0.002); and nitrogen metabolism (0.11 ± 0.03, p = 0.003). Introduction of empagliflozin was associated with a decrease in adenine, and an increase in cysteine and methionine metabolism (0.31 ± 0.13, p = 0.02) when maintained under euglycemia and a decrease in nitrogen metabolism under hyperglycemia (-0.07 ± 0.04, p = 0.04). Our findings show that SGLT2 inhibition counteracts the hyperglycemia-induced changes in plasma amino acid metabolism, potentially improving energy efficiency and metabolic health, though more research is needed to confirm these metabolic effects.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 16","pages":"e70465"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12371268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute metabolite responses to swimming exercise of different intensities in highly trained male and female swimmers. 高度训练的男女游泳运动员不同强度游泳运动的急性代谢物反应。

IF 2.2

Physiological Reports Pub Date : 2025-08-01 DOI: 10.14814/phy2.70532

Andew D Govus, Lachlan J G Mitchell, Chloe D Goldsmith, Katie E McGibbon, David B Pyne, Maria Kozlovskaia, Nathan G Lawler

{"title":"Acute metabolite responses to swimming exercise of different intensities in highly trained male and female swimmers.","authors":"Andew D Govus, Lachlan J G Mitchell, Chloe D Goldsmith, Katie E McGibbon, David B Pyne, Maria Kozlovskaia, Nathan G Lawler","doi":"10.14814/phy2.70532","DOIUrl":"https://doi.org/10.14814/phy2.70532","url":null,"abstract":"We investigated metabolite responses to different swimming intensities in 16 highly trained swimmers (9 males, 7 females, aged 16-24 years). After determining critical swimming speed (CS) with a 12 × 25 m maximal effort test, participants completed three swimming trials at moderate (below CS), heavy (at CS), and severe (above CS) intensities on separate days. Capillary blood samples (1 mL) were collected before and after each trial for metabolite profiling via mass spectrometry. Orthogonal partial least squares analysis (OPLS-DA) revealed distinct metabolite changes between moderate and severe intensity trials [R2X (cum): 0.56; R2Y(cum): 0.95, pR2Y: 0.02, pQ2: 0.02]. Free fatty acids (FFAs) 18:0, 20:0, 20:4, and 22:4 showed higher log2 fold changes (log2FC) after moderate compared to heavy and severe trials (all p < 0.01). Plasma lactate, pyruvate, alanine, and HDL-4 cholesterol concentrations had greater log2FC after heavy and severe trials than moderate (all p < 0.01). Males tended to show lower log2FC in FFA than females in the severe trial, though this was not significant. These findings demonstrate that swimming intensity influences metabolite profiles, with reduced lipid metabolism and increased TCA cycle activity as intensity rises. A 1 mL capillary blood sample can effectively capture these metabolic shifts.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 16","pages":"e70532"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12381355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dose-response effects of hydrolyzed curcumin on recovery, inflammation, and oxidative stress following exercise-induced muscle damage in males. 水解姜黄素对男性运动引起的肌肉损伤后的恢复、炎症和氧化应激的剂量反应效应。

IF 2.2

Physiological Reports Pub Date : 2025-08-01 DOI: 10.14814/phy2.70504

Toby A Helder, Sean Condon, Ian Grant, Nathan A Lewis, Jessica Hill, Charles R Pedlar, Luke Hughes

{"title":"The dose-response effects of hydrolyzed curcumin on recovery, inflammation, and oxidative stress following exercise-induced muscle damage in males.","authors":"Toby A Helder, Sean Condon, Ian Grant, Nathan A Lewis, Jessica Hill, Charles R Pedlar, Luke Hughes","doi":"10.14814/phy2.70504","DOIUrl":"10.14814/phy2.70504","url":null,"abstract":"This study investigated the effects of two dosages (750 mg and 1500 mg) of hydrolyzed curcumin on physiological recovery following exercise-induced muscle damage (EIMD). In a randomized, placebo-controlled, double-blind design, 34 recreationally active males (27 ± 6 years; 180 ± 7.3 cm; 82 ± 11.3 kg) were assigned to three groups: PLA (2 × 750-mg/day placebo), LOW (1 × 750-mg/day curcumin + 1 × 750-mg/day placebo), and HIGH (2 × 750-mg/day curcumin). Supplements were delivered in 15 mL gel sachets over 7 days, starting 48 h before EIMD. The EIMD protocol involved 8 sets of 10 repetitions at 110% of one-repetition maximum on the leg press, with 5-s eccentric phases and assisted concentric phases, targeting the quadriceps. Recovery was assessed pre, post, 24, 48, and 72 h post-EIMD via the Free Oxygen Radical Test (FORT), creatine kinase (CK), interleukin-6 (IL-6), isokinetic peak power, and a muscle endurance test (sustained isometric contraction at 50% peak torque). The HIGH group showed significantly greater reductions in pain, CK, FORT, and IL-6 (p < 0.05), but slower muscle endurance recovery at 24 h compared to LOW. Findings suggest a dose-response effect, with higher curcumin doses improving biochemical recovery but potentially impairing performance recovery.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 15","pages":"e70504"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12334844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Norepinephrine inhibits cell cycle re-entry of neonatal rat ventricular cardiomyocytes characterized by the absence of de novo nestin expression. 去甲肾上腺素抑制新生大鼠心室心肌细胞的细胞周期再进入，其特征是没有新生巢素的表达。

IF 2.2

Physiological Reports Pub Date : 2025-08-01 DOI: 10.14814/phy2.70488

Adrien Aubry, Mariana Kebbe, Aya Al-Katat, Louis Villeneuve, Angelino Calderone

{"title":"Norepinephrine inhibits cell cycle re-entry of neonatal rat ventricular cardiomyocytes characterized by the absence of de novo nestin expression.","authors":"Adrien Aubry, Mariana Kebbe, Aya Al-Katat, Louis Villeneuve, Angelino Calderone","doi":"10.14814/phy2.70488","DOIUrl":"10.14814/phy2.70488","url":null,"abstract":"The p38α/β MAPK inhibitor SB203580 in the presence of a phorbol ester (protein kinase C activator) induced cell cycle re-entry of two subpopulations of neonatal rat ventricular cardiomyocytes (NNVMs) distinguished by the absence or de novo nestin expression. Recent studies have reported that the β-adrenergic receptor inhibited the cell cycle re-entry of ventricular cardiomyocytes. The present study tested the hypothesis that sympathetic recruitment of p38α/β MAPK signaling suppressed cell cycle re-entry of one or both subpopulations of 1-day-old NNVMs. Norepinephrine (NE; 1 μM) or Isoproterenol (ISO; 1 μM) treatment of NNVMs for 24 h significantly attenuated 5-bromo-2'-deoxyuridine incorporation, and NE increased p38α MAPK phosphorylation. SB203580 (10 μM) treatment of NE/ISO-stimulated NNVMs selectively re-established the cell cycle re-entry of the subpopulation distinguished by the absence of de novo nestin expression. Phorbol 12,13-dibutyrate (PDBu; 100 nM) treatment attenuated 5-bromo-2'-deoxyuridine incorporation, and SB203580 treatment predominantly initiated re-entry into the cell cycle of the NNVM subpopulation characterized by de novo nestin expression. siRNA-mediated PKC-α protein downregulation in NNVMs selectively suppressed PDBu/SB203580-mediated de novo nestin and subsequent cell cycle re-entry. Thus, sympathetic stimuli acting via the β1/2-adrenergic receptor selectively inhibited the cell cycle re-entry of the nestin(-)-NNVM subpopulation via recruitment of p38α/β MAPK.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 15","pages":"e70488"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12309976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144754033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic time course of skeletal muscle disuse and rehabilitation in middle-aged adults. 中年人骨骼肌失用与康复的转录组学时间过程。

IF 2.2

Physiological Reports Pub Date : 2025-08-01 DOI: 10.14814/phy2.70497

Zachary D Von Ruff, Sean P Kilroe, Erik D Marchant, Emily J Arentson-Lantz, Steven Widen, Jill Thompson, Alejandro Villasante-Tezanos, Elena Volpi, Doug Paddon-Jones, Blake B Rasmussen

{"title":"Transcriptomic time course of skeletal muscle disuse and rehabilitation in middle-aged adults.","authors":"Zachary D Von Ruff, Sean P Kilroe, Erik D Marchant, Emily J Arentson-Lantz, Steven Widen, Jill Thompson, Alejandro Villasante-Tezanos, Elena Volpi, Doug Paddon-Jones, Blake B Rasmussen","doi":"10.14814/phy2.70497","DOIUrl":"10.14814/phy2.70497","url":null,"abstract":"Disuse drives rapid muscle atrophy and metabolic dysfunction. This study aimed to characterize phenotypic and transcriptomic skeletal muscle changes in middle-aged individuals during disuse and rehabilitation. Eleven healthy middle-aged adults (6 males, 5 females; age; 57 ± 5 years) underwent 7 days of unilateral lower limb suspension (ULLS). Following disuse, participants participated in a rehabilitation program consisting of either a lower-body resistance exercise (RE) or walking control (WC) three times weekly for 2 weeks. Bilateral skeletal muscle biopsies were collected at Day 0 and Day 7 of disuse and 2 h post-exercise on Days 7, 9, 11, and 21. Strength testing was conducted, and RNA sequencing was performed on muscle samples. Seven days of disuse reduced knee extension strength (14%; p < 0.05) and isometric force (13%; p < 0.05). Over-representation analysis revealed a downregulation of mRNAs related to cellular respiration and NADH dehydrogenase complex assembly. Resistance exercise induced robust, but different, transcriptional changes in both disuse- and control-legs. Walking had minimal effect on the muscle transcriptome. We conclude that 7 days of disuse reduced leg strength, decreased mitochondrial gene expression, and increased inflammation and apoptosis-related genes. We also conclude that resistance exercise enhanced recovery from disuse by improving strength, associated with significant transcriptomic changes.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 15","pages":"e70497"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144795089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0