Physiological Reports最新文献_第10页

Associations between skeletal muscle phenotype, positional role, and on-ice performance in elite male ice hockey players. 精英男子冰球运动员骨骼肌表型、位置角色和冰上表现之间的关联。

IF 2.2

Physiological Reports Pub Date : 2024-11-01 DOI: 10.14814/phy2.70081

Jeppe F Vigh-Larsen, Hallur Thorsteinsson, Martin Thomassen, Jeppe Panduro, Bjørn Fristrup, Morten B Randers, Jens L Olesen, Peter Krustrup, Kristian Overgaard, Lars Nybo, Magni Mohr

{"title":"Associations between skeletal muscle phenotype, positional role, and on-ice performance in elite male ice hockey players.","authors":"Jeppe F Vigh-Larsen, Hallur Thorsteinsson, Martin Thomassen, Jeppe Panduro, Bjørn Fristrup, Morten B Randers, Jens L Olesen, Peter Krustrup, Kristian Overgaard, Lars Nybo, Magni Mohr","doi":"10.14814/phy2.70081","DOIUrl":"10.14814/phy2.70081","url":null,"abstract":"We evaluated associations between muscle phenotype, positional role, and on-ice performance in male U20 Danish national team ice hockey players. Sixteen players (10 forwards, six defensemen) participated in a game with activity tracking. Resting thigh muscle biopsies were analyzed for metabolic enzyme activity and protein expression linked to performance. On-ice intermittent exercise capacity, repeated sprint ability, and maximal isometric knee-extensor torque were also assessed. No significant position-specific muscle phenotype characteristics were found, but forwards generally exhibited higher levels of several membrane proteins (p = 0.100-0.991). NAKα2, NAK∑, KATP, ClC-1, and NHE1 showed significant correlations with total distance (r = 0.52-0.59, p = 0.016-0.046), however, within positions these only persisted for KATP (r = 0.70, p = 0.024) and NAKα2 (r = 0.57, p = 0.085) in forwards, where CS enzyme activity also displayed a strong association with distance covered (r = 0.75, p = 0.019). For high-intensity skating, NAKα2 (r = 0.56, p = 0.025) and KATP (r = 0.50, p = 0.048) similarly exhibited the strongest associations, persisting within forwards (r = 0.63, p = 0.052 and r = 0.72; p = 0.018, respectively). In conclusion, although several muscle proteins involved in ion and metabolic regulation were associated with performance, only NAKα2 and KATP displayed consistent relationships within positions. Moreover, CS enzyme activity was strongly related to total distance within forwards, coherent with the proposed importance of oxidative capacity in intense intermittent exercise.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 21","pages":"e70081"},"PeriodicalIF":2.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of exposure duration on human pressure-induced inflammatory responses: Comparison between tunnel workers and underwater divers. 暴露时间对人体压力引起的炎症反应的影响：隧道工人与水下潜水员的比较。

IF 2.2

Physiological Reports Pub Date : 2024-11-01 DOI: 10.14814/phy2.70130

Zuha Imtiyaz, Owen J O'Neill, Douglas Sward, Phi-Nga Jeannie Le, Awadhesh K Arya, Veena M Bhopale, Abid R Bhat, Stephen R Thom

{"title":"Influence of exposure duration on human pressure-induced inflammatory responses: Comparison between tunnel workers and underwater divers.","authors":"Zuha Imtiyaz, Owen J O'Neill, Douglas Sward, Phi-Nga Jeannie Le, Awadhesh K Arya, Veena M Bhopale, Abid R Bhat, Stephen R Thom","doi":"10.14814/phy2.70130","DOIUrl":"10.14814/phy2.70130","url":null,"abstract":"Information is scarce on human responses to high pressure exposures out of water, such as related to tunnel construction workers. We hypothesized that differences in the longer durations of exposures for tunnel workers versus underwater divers results in greater inflammatory responses linked to the pathophysiology of decompression sickness (DCS). Blood was analyzed from 15 tunnel workers (36.1 ± 10.5 (SD) years old, 6 women) exposed to 142-156 kPa pressure for 4.1-4.9 h compared to 8 SCUBA divers (39.3 ± 13.3 (SD) years old, 6 women) exposed to 149 kPa for 0.61 hours. Despite differences in pressure duration between groups, elevations were the same for blood microparticles (MPs) (128 ± 28% MPs/μl) and intra-MPs interleukin (IL-1β) (376 ± 212% pg/million MPs), and for decreases of plasma gelsolin (pGSN, 31 ± 27% μg/mL). The number of circulating CD66b + neutrophils and evidence of cell activation, insignificant for divers, increased in tunnel workers. Across 3 exposures, the mean neutrophil count increased 150 ± 11%. Neutrophil activation increased by 1 to 2% of cells expressing cell surface CD18, myeloperoxidase, platelet-specific CD41, and decrease of cell bound pGSN. We conclude that MPs elevations occur rapidly in humans and reach steady state in minutes with pressure exposures and neutrophil activation requires significantly longer exposure times.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 22","pages":"e70130"},"PeriodicalIF":2.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11578652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perturbations of tryptophan catabolism via the kynurenine pathway are associated with stage 2 postoperative outcomes in single ventricle heart disease. 色氨酸通过犬尿氨酸途径分解的干扰与单心室心脏病术后第二阶段的结果有关。

IF 2.2

Physiological Reports Pub Date : 2024-11-01 DOI: 10.14814/phy2.70133

Jennifer Romanowicz, Sierra Niemiec, Ludmila Khailova, Tanner Lehmann, Christopher A Mancuso, Max B Mitchell, Gareth J Morgan, Mark Twite, Michael V DiMaria, Jelena Klawitter, Jesse A Davidson, Benjamin S Frank

{"title":"Perturbations of tryptophan catabolism via the kynurenine pathway are associated with stage 2 postoperative outcomes in single ventricle heart disease.","authors":"Jennifer Romanowicz, Sierra Niemiec, Ludmila Khailova, Tanner Lehmann, Christopher A Mancuso, Max B Mitchell, Gareth J Morgan, Mark Twite, Michael V DiMaria, Jelena Klawitter, Jesse A Davidson, Benjamin S Frank","doi":"10.14814/phy2.70133","DOIUrl":"10.14814/phy2.70133","url":null,"abstract":"Preliminary evidence suggests perturbations of the kynurenine pathway (KP) of tryptophan metabolism in infants with single ventricle heart disease (SVHD). In 72 infants with SVHD undergoing stage 2 palliation (S2P) and 41 controls, we quantified serum KP metabolite concentrations via tandem mass spectroscopy pre-S2P and post-S2P at 2, 24, and 48 h and assessed metabolite relationships with post-S2P outcomes (length of stay, hypoxemia burden, and intubation duration). Pre-S2P, SVHD infants had lower tryptophan and serotonin levels and higher kynurenic acid, 3-hydroxykynurenine, and picolinic acid levels than controls. Post-S2P, metabolites peaked at 2 h, with return to baseline by 48 h for all except kynurenic acid, which remained elevated. Metabolite concentrations pre-S2P were poorly associated with outcomes. A lower serotonin peak 2 h post-S2P was associated with longer length of stay and intubation duration. Multiple metabolites at 24 and 48 h correlated with outcomes; notably, elevated kynurenic acid was associated with worse results for all three outcomes. Our results confirm that interstage SVHD infants have altered KP activity compared to controls. Further, the link between outcomes and KP metabolites post-S2P-but not at baseline-demonstrates that acute, perioperative changes in tryptophan catabolism may be more important to tolerating S2P physiology than chronic interstage changes.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 22","pages":"e70133"},"PeriodicalIF":2.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitigating hemoglobin-induced nephropathy: ApoHb-hp protection of podocytes. 减轻血红蛋白诱发的肾病：保护荚膜细胞的载脂蛋白-hp

IF 2.2

Physiological Reports Pub Date : 2024-11-01 DOI: 10.14814/phy2.70132

Daniela Lucas, Carlos Munoz, Quintin O'Boyle, Ivan S Pires, Andre F Palmer, Pedro Cabrales

{"title":"Mitigating hemoglobin-induced nephropathy: ApoHb-hp protection of podocytes.","authors":"Daniela Lucas, Carlos Munoz, Quintin O'Boyle, Ivan S Pires, Andre F Palmer, Pedro Cabrales","doi":"10.14814/phy2.70132","DOIUrl":"10.14814/phy2.70132","url":null,"abstract":"This study investigates hemoglobin (Hb)-induced kidney injury and the protective role of the ApoHemoglobin-Haptoglobin (ApoHb-Hp) complex against heme and Hb damage. Hb facilitates oxygen (O2) delivery but poses challenges outside red blood cells (RBCs) due to toxic Hb and heme mechanisms. These are managed by binding to serum proteins like Haptoglobin (Hp) and Hemopexin (Hpx). During hemolysis, depletion of Hp and Hpx leaves tissues vulnerable to Hb and heme. To address this, we developed the ApoHb-Hp complex, based on Apohemoglobin, which is produced by removing heme from Hb, conjugated with Hp. This complex acts as a dual scavenger for Hb and heme, preventing tissue damage. Our findings demonstrate that ApoHb-Hp significantly protects MPC5 podocytes from Hb-induced damage. Fluorescent staining showed a higher percentage of nephrin-positive cells in the ApoHb-Hp group, and MTT assays revealed enhanced cell viability compared to Hb alone. Additionally, ApoHb-Hp reduced reactive oxygen species (ROS) production, with the Hb group exhibiting significantly elevated ROS levels. The ApoHb-Hp complex mitigated the depletion of protective mechanisms, as shown by significant increases in superoxide dismutase (SOD) and glutathione (GSH). Moreover, ApoHb-Hp treatment reduced the activation of the NLRP3 inflammasome signaling pathway and inflammatory cytokines IL-1β and IL-18. These findings underscore the therapeutic potential of ApoHb-Hp in mitigating Hb-induced renal damage by preserving podocyte viability and reducing oxidative stress. Overall, ApoHb-Hp maintained protective mechanisms depleted otherwise by Hb. These findings highlight ApoHb-Hp's potential as a therapeutic agent against Hb-induced renal damage, offering insights into its mechanisms and implications for treating conditions involving hemolysis.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 22","pages":"e70132"},"PeriodicalIF":2.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glenn circulation causes early and progressive shunting in a surgical model of pulmonary arteriovenous malformations. 格伦循环在肺动静脉畸形手术模型中引起早期和进行性分流。

IF 2.2

Physiological Reports Pub Date : 2024-11-01 DOI: 10.14814/phy2.70123

Tina C Wan, Henry Rousseau, Carol Mattern, Madeline Tabor, Matthew R Hodges, Ramani Ramchandran, Andrew D Spearman

{"title":"Glenn circulation causes early and progressive shunting in a surgical model of pulmonary arteriovenous malformations.","authors":"Tina C Wan, Henry Rousseau, Carol Mattern, Madeline Tabor, Matthew R Hodges, Ramani Ramchandran, Andrew D Spearman","doi":"10.14814/phy2.70123","DOIUrl":"10.14814/phy2.70123","url":null,"abstract":"Pulmonary arteriovenous malformations (PAVMs) universally develop in patients with single ventricle congenital heart disease. Single ventricle PAVMs have been recognized for over 50 years but remain poorly understood. To improve our understanding, we developed a surgical rat model of Glenn circulation and characterized PAVM physiology over multiple time points. We performed a left thoracotomy and end-to-end anastomosis of the left superior vena cava to the left pulmonary artery (unilateral Glenn), or sham surgical control. To assess PAVM physiology, we quantified intrapulmonary shunting using two independent methods (bubble echocardiography and fluorescent microsphere injection). Additionally, we performed arterial blood gas measurements to assess oxygenation and plethysmography to assess ventilation. We identified pathologic intrapulmonary shunting by bubble echocardiography as early as 2 weeks post-Glenn, and shunting continued at 2- and 6-months post-Glenn. Shunting also progressed over time, demonstrated by increased shunting of 10 μm microspheres at 6 months. Shunting was accompanied by mildly decreased oxygenation but no differences in ventilation. Our surgical animal model of unilateral Glenn circulation recreates the clinical condition of single ventricle PAVMs with early and progressive intrapulmonary shunting. This model is poised to characterize single ventricle PAVM pathophysiology and lead to mechanistic and therapeutic discovery.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 22","pages":"e70123"},"PeriodicalIF":2.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circ_0089761 accelerates colorectal cancer metastasis and immune escape via miR-27b-3p/PD-L1 axis. Circ_0089761通过miR-27b-3p/PD-L1轴加速结直肠癌转移和免疫逃逸。

IF 2.2

Physiological Reports Pub Date : 2024-11-01 DOI: 10.14814/phy2.70137

Qizhong Gao, Xiaowei Cheng, Xiang Gao

{"title":"Circ_0089761 accelerates colorectal cancer metastasis and immune escape via miR-27b-3p/PD-L1 axis.","authors":"Qizhong Gao, Xiaowei Cheng, Xiang Gao","doi":"10.14814/phy2.70137","DOIUrl":"10.14814/phy2.70137","url":null,"abstract":"Circular RNAs have been implicated as critical regulators in the initiation and progression of colorectal cancer (CRC). This study was intended to elucidate the functional significance of the circ_0089761/miR-27b-3p/programmed cell death ligand 1 (PD-L1) axis in CRC. Our findings indicated that circ_0089761 expression was significantly elevated in CRC tissues and cell lines. Furthermore, the high expression of circ_0089761 was correlated with TNM stage and tumor size. Silencing circ_0089761 inhibited CRC cell proliferation, migration, and invasion, and increased apoptosis. Mechanistically, circ_0089761 facilitated its biological function by binding to miR-27b-3p to upregulate PD-L1 expression in CRC. Coculture experiments confirmed that low expression of circ_0089761 impeded CD8 + T cell apoptosis and depletion, activated CD8 + T cell function, and increased secretion of the immune effector cytokines IFN-γ, TNF-α, perforin, and granzyme-B. MiR-27b-3p inhibition or PD-L1 overexpression partially impeded CD8 + T cell function. The circ_0089761/miR-27b-3p/PD-L1 axis is postulated to exert pivotal functions in the mechanistic progression of CRC. Furthermore, it holds promising prospects as a feasible biomarker and therapeutic target for CRC.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 23","pages":"e70137"},"PeriodicalIF":2.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11617067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the repeatability of expiratory flow limitation during incremental exercise in healthy adults. 评估健康成年人在增量运动中呼气流量限制的可重复性。

IF 2.2

Physiological Reports Pub Date : 2024-10-01 DOI: 10.14814/phy2.70068

Jack R Dunsford, Jasvir K Dhaliwal, Gracie O Grift, Robert Pryce, Paolo B Dominelli, Yannick Molgat-Seon

{"title":"Assessing the repeatability of expiratory flow limitation during incremental exercise in healthy adults.","authors":"Jack R Dunsford, Jasvir K Dhaliwal, Gracie O Grift, Robert Pryce, Paolo B Dominelli, Yannick Molgat-Seon","doi":"10.14814/phy2.70068","DOIUrl":"10.14814/phy2.70068","url":null,"abstract":"We sought to determine the repeatability of EFL in healthy adults during incremental cycle exercise. We hypothesized that the repeatability of EFL would be \"strong\" when assessed as a binary variable (i.e., absent or present) but \"poor\" when assessed as a continuous variable (i.e., % tidal volume overlap). Thirty-two healthy adults performed spirometry and an incremental cycle exercise test to exhaustion on two occasions. Standard cardiorespiratory variables were measured at rest and throughout exercise, and EFL was assessed by overlaying tidal expiratory flow-volume and maximal expiratory flow-volume curves. The repeatability of EFL was determined using Cohen's κ for binary assessments of EFL and intraclass correlation (ICC) for continuous measures of EFL. During exercise, n = 12 participants (38%) experienced EFL. At peak exercise, the repeatability of EFL was \"minimal\" (κ = 0.337, p = 0.145) when assessed as a binary variable and \"poor\" when measured as a continuous variable (ICC = 0.338, p = 0.025). At matched levels of minute ventilation during high-intensity exercise (i.e., >75% of peak oxygen uptake), the repeatability of EFL was \"weak\" when measured as a binary variable (κ = 0.474, p = 0.001) and \"moderate\" when measured as a continuous variable (ICC = 0.603, p < 0.001). Our results highlight the day-to-day variability associated with assessing EFL during exercise in healthy adults.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 19","pages":"e70068"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11446834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Submaximal eccentric resistance training increases serial sarcomere number and improves dynamic muscle performance in old rats. 亚极限偏心阻力训练可增加老年大鼠的序列肌节数量并改善动态肌肉性能。

IF 2.2

Physiological Reports Pub Date : 2024-10-01 DOI: 10.14814/phy2.70036

Avery Hinks, Ethan Vlemmix, Geoffrey A Power

{"title":"Submaximal eccentric resistance training increases serial sarcomere number and improves dynamic muscle performance in old rats.","authors":"Avery Hinks, Ethan Vlemmix, Geoffrey A Power","doi":"10.14814/phy2.70036","DOIUrl":"10.14814/phy2.70036","url":null,"abstract":"The age-related loss of muscle mass is partly accounted for by the loss of sarcomeres in series, contributing to declines in muscle mechanical performance. Resistance training biased to eccentric contractions increases serial sarcomere number (SSN) in young muscle, however, maximal eccentric training in old rats previously did not alter SSN and worsened performance. A submaximal eccentric training stimulus may be more conducive to adaptation for aged muscle. The purpose of this study was to assess whether submaximal eccentric training can increase SSN and improve mechanical function in old rats. Twelve 32-month-old male F344/BN rats completed 4 weeks of submaximal (60% maximum) eccentric plantar-flexion training 3 days/week. Pre- and post-training, we assessed in-vivo maximum isometric torque at a stretched and neutral ankle angle, the passive torque-angle relationship, and the isotonic torque-velocity-power relationship. The soleus and medial gastrocnemius (MG) were harvested for SSN measurements via laser diffraction, with the untrained leg as a control. SSN increased 11% and 8% in the soleus and MG, respectively. Training also shifted optimal torque production towards longer muscle lengths, reduced passive torque 42%, and increased peak isotonic power 23%. Submaximal eccentric training was beneficial for aged muscle adaptations, increasing SSN, reducing muscle passive tension, and improving dynamic contractile performance.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 19","pages":"e70036"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statin suppresses the development of excessive intimal proliferation in a Kawasaki disease mouse model. 他汀类药物可抑制川崎病小鼠模型内膜过度增殖的发展。

IF 2.2

Physiological Reports Pub Date : 2024-10-01 DOI: 10.14814/phy2.70096

Yusuke Motoji, Ryuji Fukazawa, Ryosuke Matsui, Makoto Watanabe, Yoshiaki Hashimoto, Noriko Nagi-Miura, Tadashi Kitamura, Kagami Miyaji

{"title":"Statin suppresses the development of excessive intimal proliferation in a Kawasaki disease mouse model.","authors":"Yusuke Motoji, Ryuji Fukazawa, Ryosuke Matsui, Makoto Watanabe, Yoshiaki Hashimoto, Noriko Nagi-Miura, Tadashi Kitamura, Kagami Miyaji","doi":"10.14814/phy2.70096","DOIUrl":"https://doi.org/10.14814/phy2.70096","url":null,"abstract":"Kawasaki disease (KD) causes vascular injury and lifelong remodeling. Excessive intimal proliferation has been observed, resulting in coronary artery lesions (CALs). However, the mechanisms underlying vascular remodeling in CAL and statin treatment have not been comprehensively elucidated. This study aimed to investigate the effects of statins on vascular remodeling using a KD mouse model. Candida albicans water-soluble substance (CAWS) was intraperitoneally injected in 5-week-old male apolipoprotein-E-deficient mice. They were categorized as follows (n = 4): control, CAWS, CAWS+statin, and late-statin groups. The mice were euthanized at 6 or 10 weeks after injection. Statins (atorvastatin) were initiated after CAWS injection, except for the late-statin group, for which statins were internally administered 6 weeks after injection. Elastica van Gieson staining and immunostaining were performed for evaluation. Statins substantially suppressed the marked neointimal hyperplasia induced by CAWS. Additionally, CAWS induced TGFβ receptor II and MAC-2 expression around the coronary arteries, which was suppressed by the statins. KD-like vasculitis might promote the formation of aneurysm by destroying elastic laminae and inducing vascular stenosis by neointimal proliferation. The anti-inflammatory effects of statins might inhibit neointimal proliferation. Therefore, statin therapy might be effective in adult patients with KD with CAL by inhibiting vascular remodeling.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 20","pages":"e70096"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11489001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Going with the flow: New insights regarding flow induced K⁺ secretion in the distal nephron. 顺其自然：关于远端肾小球流量诱导 K+ 分泌的新见解。

IF 2.2

Physiological Reports Pub Date : 2024-10-01 DOI: 10.14814/phy2.70087

Samia Lasaad, Andrew J Nickerson, Gilles Crambert, Lisa M Satlin, Thomas R Kleyman

{"title":"Going with the flow: New insights regarding flow induced K+ secretion in the distal nephron.","authors":"Samia Lasaad, Andrew J Nickerson, Gilles Crambert, Lisa M Satlin, Thomas R Kleyman","doi":"10.14814/phy2.70087","DOIUrl":"10.14814/phy2.70087","url":null,"abstract":"K+ secretion in the distal nephron has a critical role in K+ homeostasis and is the primary route by which K+ is lost from the body. Renal K+ secretion is enhanced by increases in dietary K+ intake and by increases in tubular flow rate in the distal nephron. This review addresses new and important insights regarding the mechanisms underlying flow-induced K+ secretion (FIKS). While basal K+ secretion in the distal nephron is mediated by renal outer medullary K+ (ROMK) channels in principal cells (PCs), FIKS is mediated by large conductance, Ca2+/stretch activated K+ (BK) channels in intercalated cells (ICs), a distinct cell type. BK channel activation requires an increase in intracellular Ca2+ concentration ([Ca2+]i), and both PCs and ICs exhibit increases in [Ca2+]i in response to increases in tubular fluid flow rate, associated with an increase in tubular diameter. PIEZO1, a mechanosensitive, nonselective cation channel, is expressed in the basolateral membranes of PCs and ICs, where it functions as a mechanosensor. The loss of flow-induced [Ca2+]i transients in ICs and BK channel-mediated FIKS in microperfused collecting ducts isolated from mice with IC-specific deletion of Piezo1 in the CCD underscores the importance of PIEZO1 in the renal regulation of K+ transport.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"12 20","pages":"e70087"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11491169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0