Physiological Reports最新文献

筛选
英文 中文
Comparing methods to measure the dispersion of breathing parameters during exercise testing: A simulation study based on real-life parameters from patients with dysfunctional breathing.
IF 2.2
Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70233
Léon Genecand, Cyril Jaksic, Roberto Desponds, Gaëtan Simian, Ivan Guerreiro, Sara Thorens, Marco Altarelli, Isabelle Frésard, Chloé Cantero, Aurélien Bringard, Antoine Beurnier, Pierantonio Laveneziana, David Montani, Anne Bergeron, Frédéric Lador, Pierre-Olivier Bridevaux
{"title":"Comparing methods to measure the dispersion of breathing parameters during exercise testing: A simulation study based on real-life parameters from patients with dysfunctional breathing.","authors":"Léon Genecand, Cyril Jaksic, Roberto Desponds, Gaëtan Simian, Ivan Guerreiro, Sara Thorens, Marco Altarelli, Isabelle Frésard, Chloé Cantero, Aurélien Bringard, Antoine Beurnier, Pierantonio Laveneziana, David Montani, Anne Bergeron, Frédéric Lador, Pierre-Olivier Bridevaux","doi":"10.14814/phy2.70233","DOIUrl":"10.14814/phy2.70233","url":null,"abstract":"<p><p>The dispersion of the tidal volume and of the breathing frequency have been used to diagnose dysfunctional breathing during cardio-pulmonary exercise testing. No validated methods to objectively describe this dispersion exist. We aimed to validate such a method. We used simulations based on real-life parameters. Moving standard deviation (MSD) and residuals from locally estimated scatterplot smoothing (LOESS) were evaluated. The precision and the bias of each tested method at rest and during exercise simulations, with and without sighs, were measured. For LOESS, a 2nd degree polynomial was used, and different spans were tested (LOESS<sub>1</sub>, LOESS<sub>0.75</sub>, and LOESS<sub>0.5</sub>). For MSD, different number of points used for the calculation were tested (MSD<sub>7</sub>, MSD<sub>11</sub>, MSD<sub>15</sub>, and MSD19). The LOESS method was globally more precise, had less bias, and was less influenced by the trend as compared to MSD in almost all simulations except for extremely low dispersion combined with extreme trends. LOESS<sub>0.75</sub> had intermediate bias and precision between LOESS<sub>0.5</sub> and LOESS<sub>1</sub> in all simulations. LOESS<sub>0.75</sub> is a method that combines high precision, low bias, and low influenceability of trends. It could be considered as the method of choice to evaluate the dispersion of breathing parameters during cardiopulmonary exercise testing.</p>","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 5","pages":"e70233"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11870078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143528173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A ketogenic-promoting beverage acutely elevates cardiac function and myocardial blood flow compared to placebo in adults: A cardiac MRI investigation.
IF 2.2
Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70208
Christopher D Crabtree, Yue Pan, Preethi Chandrasekaran, Yuwei Wang, Debbie Scandling, Teryn Bedell, Justen Stoner, Drew Decker, Madison Kackley, Bradley Robinson, Alex Buga, Yuchi Han, Jeff Volek, Orlando P Simonetti
{"title":"A ketogenic-promoting beverage acutely elevates cardiac function and myocardial blood flow compared to placebo in adults: A cardiac MRI investigation.","authors":"Christopher D Crabtree, Yue Pan, Preethi Chandrasekaran, Yuwei Wang, Debbie Scandling, Teryn Bedell, Justen Stoner, Drew Decker, Madison Kackley, Bradley Robinson, Alex Buga, Yuchi Han, Jeff Volek, Orlando P Simonetti","doi":"10.14814/phy2.70208","DOIUrl":"10.14814/phy2.70208","url":null,"abstract":"<p><p>Increasing evidence suggests cardiac function improves in healthy and failing hearts alongside circulating ketones (1-4 mM). This study characterized cardiac function and blood flow responses to a ketogenic beverage compared to a volume/calorie matched placebo with repeated imaging over 120 min. This was a two-group, placebo-controlled, acute cardiac imaging study. Adults without cardiac abnormalities underwent baseline cardiac MRI including quantitative myocardial perfusion to measure myocardial blood flow (MBF). Subjects consumed 50 g of a ketogenic-promoting beverage [bis-hexanoyl R-1-3-butanediol (BH-BD)] (BH-BD; n = 11) or a calorically/volume-matched lipid-based placebo (PL; n = 10) with cardiac MRI every 15-30 min. Following 120 min, subjects underwent a final scan including MBF measurement. R-BHB and glucose were measured at every timepoint. 120 min following BH-BD consumption, R-BHB reached 2.1 mM. Cardiac output (CO) was elevated compared to PL (p < 0.05) and increased +31% 120 min after BH-BD ingestion (p < 0.001). CO elevation was due to increased stroke volume (+11%; p = 0.02) and heart rate (+22%; p < 0.001). MBF increased 29% from baseline (p < 0.001). PL did not induce differences in cardiac parameters. 50 g BH-BD ingestion achieves exogenous ketosis and is associated with elevated MBF and CO providing evidence supporting their use as a therapeutic clinical agent.</p>","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 6","pages":"e70208"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Shape factor versus truncated cone-based quantification of quadriceps and hamstring muscle volumes-A choice between accuracy and precision.
IF 2.2
Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70263
Daniel P Fitze, Nicola Mair-Noack, Dominik Brun, Daniel Nanz, Jess G Snedeker, Jörg Spörri
{"title":"Shape factor versus truncated cone-based quantification of quadriceps and hamstring muscle volumes-A choice between accuracy and precision.","authors":"Daniel P Fitze, Nicola Mair-Noack, Dominik Brun, Daniel Nanz, Jess G Snedeker, Jörg Spörri","doi":"10.14814/phy2.70263","DOIUrl":"10.14814/phy2.70263","url":null,"abstract":"<p><p>This study aimed to determine the average location of maximal anatomical cross-sectional area of the quadriceps and hamstrings and to investigate the agreement of different muscle volume estimation methods. Magnetic resonance imaging datasets were acquired from 39 soccer players. Muscle volumes were calculated using slice-by-slice segmentation and compared with the shape factor and truncated cone-based estimates. Descriptive data were expressed as means ± standard deviations, and Bland-Altman plots were used for agreement analyses. The average location of maximal anatomical cross-sectional area was at 61 ± 10%, 64 ± 10%, 29 ± 3%, and 56 ± 6% for the vastus lateralis, rectus femoris, vastus medialis, and vastus intermedius, respectively. For the hamstrings, the maximal anatomical cross-sectional area was at 45 ± 3%, 48 ± 9%, 58 ± 7%, and 38 ± 8% for the biceps femoris short head, biceps femoris long head, semitendinosus, and semimembranosus, respectively. Relative biases ranged from 2% to 9% for the shape factor and from 6% to 14% for the truncated cone method. The ranges of agreement were -15% to 29% and -1% to 19%, respectively. The shape factor method showed better accuracy, while the truncated cone method displayed better precision.</p>","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 6","pages":"e70263"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The impact of dietary sodium and fructose on renal sodium handling and blood pressure in healthy adults.
IF 2.2
Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70284
Ronald K McMillan, Joseph M Stock, Nathan T Romberger, Megan M Wenner, Sheau C Chai, William B Farquhar
{"title":"The impact of dietary sodium and fructose on renal sodium handling and blood pressure in healthy adults.","authors":"Ronald K McMillan, Joseph M Stock, Nathan T Romberger, Megan M Wenner, Sheau C Chai, William B Farquhar","doi":"10.14814/phy2.70284","DOIUrl":"10.14814/phy2.70284","url":null,"abstract":"<p><p>Increased dietary sodium is linked to hypertension, but most young adults display \"sodium-resistant\" blood pressure (BP), meaning BP is not elevated with sodium loading. In sodium-resistant rodents, fructose induces salt-sensitive BP via increased renal sodium reabsorption. Therefore, we tested the impact of fructose and sodium on renal sodium handling and BP in healthy adults, hypothesizing that their combination would impair sodium excretion and increase BP. Thirty-six participants enrolled in a randomized, double-blind, crossover trial involving three diets varying in fructose and sodium. On day 7, participants wore ambulatory BP monitors and collected 24-h urine. Although high sodium increased urinary sodium excretion, excretion was 15% lower with high fructose plus high salt versus high salt alone (235.1 ± 85.0 vs. 277.9 ± 121.2 mmol/24 h, p = 0.05). Compared to the recommended diet, high salt alone did not significantly change 24 h. MAP; however, high fructose plus high salt modestly raised 24 h MAP (81 ± 6 vs. 84 ± 7 mmHg, p = 0.03). High fructose and high salt increased serum interleukin-6 concentrations compared to the recommended diet (0.31 ± 0.2 vs. 0.24 ± 0.19 pg/mL, p = 0.04). These findings suggest that increased sodium and fructose alter renal sodium handling and BP in young adults.</p>","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 6","pages":"e70284"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Titin is a new factor regulating arterial stiffness through vascular smooth muscle cell tone in male rats.
IF 2.2
Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70270
Chaoqun Zhu, Terrance Bishop, Zachery R Gregorich, Wei Guo
{"title":"Titin is a new factor regulating arterial stiffness through vascular smooth muscle cell tone in male rats.","authors":"Chaoqun Zhu, Terrance Bishop, Zachery R Gregorich, Wei Guo","doi":"10.14814/phy2.70270","DOIUrl":"10.14814/phy2.70270","url":null,"abstract":"<p><p>Arterial stiffness is a robust predictor of cardiovascular disease and mortality. As such, there is substantial interest in uncovering its causal factors for the development of targeted treatments to regulate arterial stiffness. The elastic protein titin is a key determinant of myocardial stiffness, yet whether it plays a role in regulating arterial stiffness is unknown. In this study, we aimed to investigate the role of titin in vascular smooth muscle cell (VSMC) and overall arterial stiffness. To do this, we took advantage of rats lacking RNA binding motif 20 (RBM20), the primary splicing regulator of titin, in striated muscles. Using this model, we demonstrate that RBM20 regulates titin isoform expression in smooth muscle, with loss of the protein leading to the expression of larger titin isoforms. We show that the expression of larger titin reduces the stiffness of VSMCs. While decreased titin-based VSMC stiffness did not affect baseline arterial stiffness, we found that arterial stiffness was reduced in response to a challenge with the potent vasoconstrictor angiotensin II (Ang II). The observed reduction in arterial stiffness following Ang II treatment was not the result of changes in either the extracellular matrix or myofilaments. We further show that the expression of a larger titin isoform ameliorates cardiac remodeling caused by Ang II-associated hypertension. In summary, our study provides the first evidence that titin regulates VSMC stiffness, which is relevant for arterial stiffness in the context of elevated blood pressure. Furthermore, our data provide proof-of-concept evidence that targeting RBM20 to reduce arterial stiffness through titin isoform switching may benefit aging- or hypertension-associated arterial stiffness and vascular diseases.</p>","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 6","pages":"e70270"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928681/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corticomuscular and intermuscular coherence during evidence accumulation in sensorimotor decision-making.
IF 2.2
Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70237
Yvonne F Visser, W Pieter Medendorp, Luc P J Selen
{"title":"Corticomuscular and intermuscular coherence during evidence accumulation in sensorimotor decision-making.","authors":"Yvonne F Visser, W Pieter Medendorp, Luc P J Selen","doi":"10.14814/phy2.70237","DOIUrl":"10.14814/phy2.70237","url":null,"abstract":"<p><p>Evidence accumulation processes during decision-making are thought to continuously feed into the motor system, preparing multiple competing motor plans, of which one is executed when the evidence is complete. Previously, the state of this accumulation process has been studied by reading out the preparatory state of the motor system with evoked responses, once per trial. In this study, we aim to continuously track the sensorimotor decision during the trial using corticomuscular (CMC) and intermuscular coherence (IMC). We recorded EEG and EMG of healthy young adults (n = 34) who viewed random dot motion stimuli, with varying strengths across trials, and indicated their perceived motion direction by reaching towards one of two targets, requiring either flexion or extension of the elbow. Coherence was computed in the beta band. After stimulus presentation, both CMC and IMC show an initial phasic pattern, which is followed by sustained coherence patterns at a level that depends on stimulus strength for CMC. Prior to reach onset, the CMC for different stimulus strengths had a tendency to settle at similar levels. This tendency tentatively marks a stimulus-independent decision bound. We conclude that CMC, and to a lesser extent IMC, track the evidence accumulation process on a single trial.</p>","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 6","pages":"e70237"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cold pressor-induced sympathetic activation blunts the femoral but not carotid artery vascular responsiveness.
IF 2.2
Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70281
Guilherme F Speretta, Gaia Giuriato, Gianluigi Dorelli, Chiara Barbi, Anna Pedrinolla, Massimo Venturelli
{"title":"Cold pressor-induced sympathetic activation blunts the femoral but not carotid artery vascular responsiveness.","authors":"Guilherme F Speretta, Gaia Giuriato, Gianluigi Dorelli, Chiara Barbi, Anna Pedrinolla, Massimo Venturelli","doi":"10.14814/phy2.70281","DOIUrl":"10.14814/phy2.70281","url":null,"abstract":"<p><p>Vascular responsiveness due to passive leg movement (PLM) on the brain remains unknown. This study aimed to evaluate the effects of cold-induced sympathetic activation (CPT) on femoral and ipsilateral and contralateral carotid arteries' vascular responsiveness evoked by PLM. Thirteen participants (seven males and six females; age: 27.0 ± 2.3 years) undertook a randomized session in which PLM was performed on the right leg at rest and during CPT. Right femoral (fBF) and right (ipsilateral) and left (contralateral) carotid (cBF) blood flows were measured by ultrasounds, and heart hemodynamics were assessed via photoplethysmography and impedance cardiograph. Systolic arterial pressure (SAP) time series were used to infer sympathetic modulation to the vessels. Femoral (fVC) and carotid (cVC) vascular conductance (BF/MAP) were calculated. CPT evoked changes in PLM on cBF, fBF, and fVC (interaction and time effect). cBF peak and cBF and cVC area under the curve were higher in the contralateral carotid in the two interventions. Low-frequency power of SAP was higher in PLM-CPT than in PLM; all p < 0.05. These results suggest that the CPT-induced increases in sympathetic modulation attenuate the vascular responsiveness in the femoral, but not the carotid, arteries. Also, the contralateral carotid increased blood flow during PLM, regardless of the CPT.</p>","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 6","pages":"e70281"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evidence of myogenic vasoconstriction in human bone vasculature.
IF 2.2
Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70278
Adina E Draghici, Matthew R Ely, Jason W Hamner
{"title":"Evidence of myogenic vasoconstriction in human bone vasculature.","authors":"Adina E Draghici, Matthew R Ely, Jason W Hamner","doi":"10.14814/phy2.70278","DOIUrl":"10.14814/phy2.70278","url":null,"abstract":"<p><p>Despite the critical importance of blood flow for bone, mechanisms regulating bone vasculature are poorly understood. Myogenic vasoconstriction is an important regulatory mechanism that is engaged in most daily activities, but our understanding primarily derives from animal work and/or other vascular beds. In young healthy adults, we employed two levels of leg dependency to engage myogenic vasoconstriction. We measured tibial blood content via near-infrared spectroscopy (total hemoglobin, ΔtHb) and contrasted it to whole leg flow via popliteal blood flow velocity (LBV) via Doppler ultrasound. Myogenic vasoconstriction was engaged by lowering the leg below heart level (supine to upright to dependent), resulting in increased leg perfusion pressure as assessed by brachial mean pressure adjusted for the hydrostatic pressure from the heart to the tibia. Increased leg perfusion pressure in both positions (Δ30.1 ± 1.36 and Δ42.1 ± 1.16 mmHg; p < 0.01) was accompanied by graded declines in LBV (Δ-1.88 ± 0.21 and Δ-2.98 ± 0.27 cm/(s*beat); p < 0.01), indicating whole limb myogenic vasoconstriction. Tibial hemoglobin content did not change (ΔtHb: -0.28 ± 1.76 and 1.26 ± 2.33 μM; p > 0.5), indicating myogenic vasoconstriction was evident, but of lower magnitude compared to the whole leg. These results indicate that myogenic vasoconstriction plays an active role in regulating the tibial vasculature, but with a less robust response compared to the whole leg.</p>","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 6","pages":"e70278"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rest-to-work and work-to-rest transients of interstitial PO2 in spinotrapezius muscle of young and old male rats.
IF 2.2
Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70260
Aleksander S Golub, William H Nugent, Roland N Pittman, Bjorn K Song
{"title":"Rest-to-work and work-to-rest transients of interstitial PO<sub>2</sub> in spinotrapezius muscle of young and old male rats.","authors":"Aleksander S Golub, William H Nugent, Roland N Pittman, Bjorn K Song","doi":"10.14814/phy2.70260","DOIUrl":"10.14814/phy2.70260","url":null,"abstract":"<p><p>Muscle function declines with age. Since the primary energy source for contraction is aerobic, this study investigated age-related changes in muscle oxygenation dynamics to: characterize PO<sub>2</sub> transients during rest-work transitions, identify age-specific differences in oxygen delivery/utilization balance, and examine the relationship between interstitial and arterial oxygen tension (PO<sub>2</sub>). Interstitial PO<sub>2</sub> was measured with a high-resolution stroboscopic phosphorescence quenching technique to map intra-contractile dynamics during changes in muscle activity-rest-to-work (RtW) and work-to-rest (WtR) in rats aged three (young) and 23 (old) months. RtW (τ<sub>w</sub>) and WtR (τ<sub>r</sub>) PO<sub>2</sub> transitions had lag periods and mono-exponential time constants. In young muscles, lag was 4 s, τ<sub>w</sub> = 9.0 ± 3.7 s, and τ<sub>r</sub> = 15.4 ± 3.9 s. For old, lag was also 4 s with increases to τ<sub>w</sub> = 15.9 ± 3.5 s and τ<sub>r</sub> = 41.4 ± 8.3 s. Resting PO<sub>2</sub>'s were higher for young than for old (66.7 ± 13.7 vs. 60.2 ± 13.0 mmHg; p < 0.05). Work reduced PO<sub>2</sub> with a greater effect on old (42.5 ± 14.0 vs. 28.3 ± 16.5 mmHg; p < 0.05). Intra-contractile measurements revealed a spike in PO<sub>2</sub> (11 mmHg amplitude for >200 ms), which was absent in old. Further, sustained exercise in young showed a rising trend in PO<sub>2</sub>, while old remained at nadir. The missing PO<sub>2</sub> spike in aged muscle contributes to reduced PO<sub>2</sub> during work and may explain age-related loss of endurance.</p>","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 5","pages":"e70260"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11867932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultrastructural analysis of mitochondrial morphology and in the human rhabdosphincter: Insights into urinary incontinence.
IF 2.2
Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70265
Shinro Hata, Mayuka Shinohara, Hiromitsu Mimata, Toshitaka Shin
{"title":"Ultrastructural analysis of mitochondrial morphology and in the human rhabdosphincter: Insights into urinary incontinence.","authors":"Shinro Hata, Mayuka Shinohara, Hiromitsu Mimata, Toshitaka Shin","doi":"10.14814/phy2.70265","DOIUrl":"10.14814/phy2.70265","url":null,"abstract":"<p><p>Urinary incontinence increases with age, reducing the elderly quality of life. Understanding its mechanisms and developing treatments are urgent tasks. While healthy striated muscle maintains homeostasis through mitophagy, aging is thought to reduce autophagy activity. This study aimed to detect abnormal mitochondrial accumulation in the rhabdosphincter using transmission electron microscopy (TEM). We collected the rhabdosphincter samples from seven patients undergoing cystectomy and used the rectus abdominis as controls. Both tissues were examined with Hematoxylin and eosin (HE) staining and TEM. ImageJ software was used to measure the mitochondrial area, perimeter, and luminance. HE staining revealed that the rhabdosphincter had fewer muscle fibers and more stromal tissue than the rectus abdominis. TEM images showed more gaps in muscle bundles and signs of mitochondrial damage, vacuolation, and swelling in the rhabdosphincter. Quantitative analysis revealed a larger average mitochondrial area (0.21 μm<sup>2</sup> vs. 0.063 μm<sup>2</sup>, p < 0.01), longer perimeter (1.83 μm vs. 0.94 μm, p < 0.01) and higher luminance (156.6 vs. 90.2, p < 0.01) than those of the rectus abdominis. The rhabdosphincter of elderly individuals showed significant mitochondrial morphological abnormalities, with increased swelling and vacuolation.</p>","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 5","pages":"e70265"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信