Physiological Reports最新文献_第3页

Acute tuft cell ablation in mice induces malabsorption and alterations in secretory and immune cell lineages in the small intestine. 小鼠急性簇细胞消减会诱发吸收不良以及小肠分泌和免疫细胞系的改变。

IF 2.2

Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70264

Michael Momoh, Francisca Adeniran, Cynthia Ramos, Kathleen E DelGiorno, Hiroshi Seno, Joseph T Roland, Izumi Kaji

{"title":"Acute tuft cell ablation in mice induces malabsorption and alterations in secretory and immune cell lineages in the small intestine.","authors":"Michael Momoh, Francisca Adeniran, Cynthia Ramos, Kathleen E DelGiorno, Hiroshi Seno, Joseph T Roland, Izumi Kaji","doi":"10.14814/phy2.70264","DOIUrl":"10.14814/phy2.70264","url":null,"abstract":"Intestinal tuft cells have recently been the focus of many studies due to their function in chemosensation and type 2 immunity in human gastrointestinal diseases. This study investigated the impact of acute tuft cell loss on intestinal physiological function. Tuft cell deletion was induced in DCLK1-IRES-GFP-CreERT2/+;Rosa-DTA (DCLK1-DTA) mice by a single tamoxifen injection, concomitant with littermate controls. Transient deletion of intestinal and biliary tuft cells was maximal on day 4 and recovered by day 7 post tamoxifen. DCLK1-DTA mice presented with significantly shortened small intestinal length and greater body weight loss by day 4. The activity of Na+-dependent glucose transporter 1 (SGLT1) and cystic fibrosis transmembrane regulator (CFTR) was reduced. Correlated with tuft cell reduction, the frequency of cholecystokinin (CCK)+ enteroendocrine and intermediate secretory cells, which co-express Paneth and goblet cell markers, was increased. In the lamina propria, fewer mast cells and leukocytes were found in the Day 4 DCLK1-DTA mice compared to controls. Ablation of tuft cells may induce nutrient malabsorption through alterations in epithelial cell proliferation and differentiation, along with changes in the mucosal defense response. These observations identify a new role for tuft cells in regulating intestinal absorption and mucosal regeneration.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 5","pages":"e70264"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterozygous GAA knockout is nonconsequential on metabolism and the spatial liver transcriptome in high-fat diet-induced obese and prediabetic mice.

IF 2.2

Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70276

Cameron P McCall, Melina C Mancini, Jaroslaw Staszkiewicz, Douglas G Mashek, Timothy D Heden

{"title":"Heterozygous GAA knockout is nonconsequential on metabolism and the spatial liver transcriptome in high-fat diet-induced obese and prediabetic mice.","authors":"Cameron P McCall, Melina C Mancini, Jaroslaw Staszkiewicz, Douglas G Mashek, Timothy D Heden","doi":"10.14814/phy2.70276","DOIUrl":"10.14814/phy2.70276","url":null,"abstract":"Glycophagy is the autophagic degradation of glycogen by the enzyme acid alpha-glucosidase (GAA). Although GAA inhibitors improve metabolic health by inhibiting GAA in the intestine, it is not clear if GAA inhibition in peripheral tissues such as the liver is metabolically beneficial. This study tested if the heterozygous knockout of GAA (HetKO-GAA) alters liver metabolism and metabolic health in mice fed a low-fat diet or a high-fat diet to induce obesity. HetKO-GAA mice fed either diet did not have altered body weight, glucose tolerance, insulin action, energy expenditure, substrate metabolism, liver glucose output, or liver triglycerides compared to control wildtype mice. A liver spatial transcriptomics analysis revealed that high-fat diet feeding reduced the gene abundance of predominantly metabolic pathways in both periportal and perivenous hepatocytes, and uniquely reduced ribosome gene abundance in perivenous hepatocytes. HetKO-GAA mice did not have significantly altered transcriptomes in periportal or perivenous hepatocytes compared to wildtype mice. In conclusion, heterozygous GAA knockout is nonconsequential on metabolism and metabolic health in high-fat diet induced obesity. Spatial transcriptomics revealed alterations in the transcriptome of periportal and perivenous hepatocytes from high-fat diet induced obese mice, highlighting novel targets that could be exploited to improve metabolic health in obesity.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 6","pages":"e70276"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11922812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infrared thermography unveiled the variation of brown adipose tissue thermogenesis among East Asian adults.

IF 2.2

Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70279

Yuka Ishida, Kazuhiro Nakayama

{"title":"Infrared thermography unveiled the variation of brown adipose tissue thermogenesis among East Asian adults.","authors":"Yuka Ishida, Kazuhiro Nakayama","doi":"10.14814/phy2.70279","DOIUrl":"10.14814/phy2.70279","url":null,"abstract":"The thermogenesis of brown adipose tissue (BAT) is interesting because the contribution to human adaptation to cold and obesity resistance has been suggested. 18F-fluorodeoxyglucose-positron emission tomography/computed tomography (FDG-PET/CT) is a common method for measuring BAT activity; however, it has been studied in few large cohorts due to concerns about safety and cost. Studies using alternative methods make it challenging to directly compare BAT activity among studies and interpret those results because the procedure is various. We measured the supraclavicular BAT thermogenesis of 122 healthy Japanese and Chinese adults under mild cold stress using standardized infrared thermography (IRT) and examined the effects of various factors on BAT variation. BAT thermogenesis was significantly higher in females than in males (p < 0.001) and significantly higher in Chinese than in Japanese individuals (p < 0.05). Among the 27 participants enrolled in both summer and winter experiments, BAT thermogenesis increased during winter (p < 0.05) only in Japanese participants. Additionally, individuals born at higher latitudes exhibited greater BAT thermogenesis (p < 0.05), suggesting the involvement of genetic background or cold exposure in early life stages. We obtained interesting anthropological and physiological findings with the use of non-invasive IRT.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 6","pages":"e70279"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of moderate static magnetic fields on voltage-gated potassium ion channels in sympathetic neuron-like PC12 cells.

IF 2.2

Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70236

Eri Kaneda, Takafumi Kawai, Yasushi Okamura, Shigeru Miyagawa

{"title":"Effects of moderate static magnetic fields on voltage-gated potassium ion channels in sympathetic neuron-like PC12 cells.","authors":"Eri Kaneda, Takafumi Kawai, Yasushi Okamura, Shigeru Miyagawa","doi":"10.14814/phy2.70236","DOIUrl":"https://doi.org/10.14814/phy2.70236","url":null,"abstract":"While exposure of moderate static magnetic fields (SMF) can alter neuronal excitability, the effects on sympathetic neurons remain underexplored. This study investigates the effects of moderate SMF on Kv channels in the plasma membrane of sympathetic neuron-like PC12 cells. The current density of Kv channels was significantly lower in the 18-h magnet-exposed group, with effects persisting even after the magnet was removed before patch-clamp measurements. The current density of outward current in the presence of TEA was not different between the two groups, indicating that magnetic field affects TEA-sensitive Kv channels. To further explore these changes, RNA sequencing was performed on samples from both the Sham and 18-h magnet-exposed groups, identifying 37 moderate SMF-sensitive genes. Changes in mRNA expression levels and KEGG analysis suggested that pathways involved in the inhibition of neuronal excitability, such as GABAB receptor activation and Kir3 channel opening, may be more likely to be activated. In conclusion, moderate SMF is strongly associated with reduced current density in PC12 cells, particularly affecting Kv channels. The present study provides fundamental information on the influence of long-term SMF exposure on the excitability of sympathetic neurons.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 6","pages":"e70236"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dextran sodium sulfate-induced colitis in male BALB/c mice leads to albuminuria and increased markers of inflammation and tissue damage in the kidney.

IF 2.2

Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70161

Hanne Salmenkari, Krishna Adeshara, Anniina Pirttiniemi, Jere Lindén, Sanna Lehtonen, Niina Sandholm, Per-Henrik Groop, Markku Lehto

{"title":"Dextran sodium sulfate-induced colitis in male BALB/c mice leads to albuminuria and increased markers of inflammation and tissue damage in the kidney.","authors":"Hanne Salmenkari, Krishna Adeshara, Anniina Pirttiniemi, Jere Lindén, Sanna Lehtonen, Niina Sandholm, Per-Henrik Groop, Markku Lehto","doi":"10.14814/phy2.70161","DOIUrl":"10.14814/phy2.70161","url":null,"abstract":"Inflammatory molecules originating from an inflamed gut can promote systemic inflammation. We studied how acute intestinal injury affects the kidneys and the kallikrein-kinin system in mice with dextran sodium sulfate (DSS)-induced colitis. Seven-week-old male BALB/c mice were treated with 5% DSS for 7 days and either sacrificed immediately (DSS7, n = 6) or given fresh water for 4 more days (DSS11, n = 6). Untreated mice (n = 6) served as controls. Colitis and kidney damage was assessed using histochemical and immunohistochemical staining, ELISA, and RT-qPCR. Markers of kidney injury correlated with markers of colitis. Colitis increased albuminuria, reduced kidney weight, and induced transcription of lipocalin 2, kidney injury molecule-1, and interleukin-1beta, as well as increased immunostaining signal of c-Jun and NF-κB p65 in the kidneys. Colitis caused strong induction of colonic kininogen 2 transcription and bradykinin receptor B1-positive cells in the disrupted mucosa. In the kidney, colitis induced localization of tubular bradykinin receptor B2 to the nuclear envelope and increased kininogen 2 transcription. Disruption of the intestinal barrier by DSS promotes markers of kidney injury and inflammation, and the degree of kidney injury correlates with the severity of colitis. Colitis is associated with increased expression of kallikrein-kinin components in both the colon and kidneys.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 5","pages":"e70161"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetomyographic assessment of pelvic floor muscles compared to ultrasound during pregnancy.

IF 2.2

Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70266

Sallie Oliphant, Luis Mercado, Eric R Siegel, Crystal Jones, Heather Moody, Diana Escalona-Vargas, Hari Eswaran

{"title":"Magnetomyographic assessment of pelvic floor muscles compared to ultrasound during pregnancy.","authors":"Sallie Oliphant, Luis Mercado, Eric R Siegel, Crystal Jones, Heather Moody, Diana Escalona-Vargas, Hari Eswaran","doi":"10.14814/phy2.70266","DOIUrl":"10.14814/phy2.70266","url":null,"abstract":"Maternal birth injury contributes to future pelvic floor disorders, yet we possess an incomplete understanding of the levator ani muscles during pregnancy. We applied a noninvasive magnetomyography technique to characterize levator ani muscle activity in pregnancy with ultrasound and clinical exam. Magnetomyographic measures of levator ani muscle activity were collected using a noninvasive biomagnetic sensor from 53 pregnant women during rest and voluntary muscle contractions of varying intensity. Power spectral density was calculated using Welch's method to obtain the mean power of each Kegel exercise. Levator hiatus circumference was measured using ultrasound, and contraction strength was measured via the Brink scale. Magnetomyography data revealed a mean root mean square (RMS) rest of 39.7 ± 8.6 femtoTesla (fT) and Kegel of 52.9 ± 17.1 fT. Mean power spectral density (PSD) in log10 (fT2/Hz) was 0.9 ± 0.2 at rest and 1.1 ± 0.2 during Kegel. Ultrasound measures of levator hiatus circumference were 13.3 ± 1.6 cm at rest and 11.6 ± 1.7 cm during maximum Kegel. Magnetomyographic correlations with levator hiatus circumference were stronger for amplitude and PSD at rest (-0.35 and -0.33) than for Kegel (-0.20 and -0.19). Magnetomyography-based amplitudes of pelvic floor activity directly correlate with ultrasound levator hiatus circumference during rest and Kegel.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 5","pages":"e70266"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11891282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of training status on muscle excitation and neuromuscular fatigue with resistance exercise with and without blood flow restriction in young men.

IF 2.2

Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70274

Brett H Davis, Guillaume Spielmann, Neil M Johannsen, Victoria Fairchild, Timothy D Allerton, Brian A Irving

{"title":"Effect of training status on muscle excitation and neuromuscular fatigue with resistance exercise with and without blood flow restriction in young men.","authors":"Brett H Davis, Guillaume Spielmann, Neil M Johannsen, Victoria Fairchild, Timothy D Allerton, Brian A Irving","doi":"10.14814/phy2.70274","DOIUrl":"10.14814/phy2.70274","url":null,"abstract":"This study compared muscle (vastus lateralis) excitation, muscle activation, and neuromuscular fatigue in response to low-load resistance exercise with blood flow restriction (LLBFR), medium-load resistance exercise with blood flow restriction (MLBFR), and high-load resistance exercise (HLRE) in resistance-trained (RT; n = 15) and untrained (UT; n = 14) college-aged males. Muscle excitation and activation were measured using surface electromyography (sEMG) and defined as the maximal root mean square amplitudes (RMS AMP) and the integrated area under the sEMG curve (iEMG) per repetition. Neuromuscular fatigue was defined as the reduction in peak torque measured during the postexercise knee extensor maximal isometric contractions (MVIC) relative to the pre-exercise MVIC. The LLBFR sessions showed 23.7% (p < 0.01) lower relative muscle excitation than the MLBFR and 26.7% (p < 0.001) lower than the HLRE. In contrast, LLBFR sessions showed 38.1% (p < 0.001) higher total muscle activation than the MLBFR and 19.3% (p < 0.05) higher than the HLRE. There were no differences between the RT and UT groups for percent change in peak torque or the RMS AMP measured during the knee extensor MVICs following the three exercise treatments (p > 0.05). However, the peak torque and maximal RMS amplitudes were higher in the RT group than in the UT group measured during the pre-exercise MVICs. Our data suggest that the LLBFR led to greater total muscle activation than MLBFR and HLRE despite lower relative muscle excitation independent of training status in our college-aged males.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 6","pages":"e70274"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Examining discordance in spirometry reference equations: A retrospective study.

IF 2.2

Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70212

Gerald S Zavorsky, Sherif Elkinany, Abdullah Alismail, Suman B Thapamagar, Michael H Terry, James D Anholm, Paresh C Giri

{"title":"Examining discordance in spirometry reference equations: A retrospective study.","authors":"Gerald S Zavorsky, Sherif Elkinany, Abdullah Alismail, Suman B Thapamagar, Michael H Terry, James D Anholm, Paresh C Giri","doi":"10.14814/phy2.70212","DOIUrl":"10.14814/phy2.70212","url":null,"abstract":"This study aimed to evaluate discordance, binary classification, and model fit between race-predicted and race-neutral spirometry prediction equations. Spirometry data from 9506 patients (18-95 years old) self-identifying as White, Black, or Hispanic were analyzed, focusing on the lower limit of normal (LLN). Best-fit prediction equations were developed from 3771 patients with normal spirometry, using Bayesian Information Criterion (BIC) to compare models with and without race as a covariate. Results showed that including race as a covariate improved model fit, reducing BIC by at least ten units compared to Race-Neutral equations. Discordance between race-specific and race-neutral equations for detecting airway obstruction and restrictive spirometry patterns ranged from 4% to 13%. Using race-neutral equations resulted in false discovery rates (FDR) of 14% for Hispanics and 45% for Blacks and false negative rates (FNR) of 21% for Hispanics and 27% for Blacks in diagnosing airway obstruction. These findings indicate that removing race as a covariate in spirometry equations increases FDR and FNR, leading to higher misclassification rates. The 4%-13% discordance in interpreting airway obstruction and restrictive patterns has significant clinical implications, underscoring the need for careful consideration in developing spirometry reference equations.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 5","pages":"e70212"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of COVID-19 severity on adult survivors: Is there a relationship between vascular reactivity and cardiorespiratory fitness?

IF 2.2

Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70216

Guilherme Dionir Back, Murilo Rezende Oliveira, Guilherme Peixoto Tinoco Arêas, Patricia Faria Camargo, Cássia da Luz Goulart, Claudio Ricardo de Oliveira, José Carlos Bonjorno Junior, Flávia Rossi Caruso Bonjourno, Ross Arena, Audrey Borghi-Silva

{"title":"The impact of COVID-19 severity on adult survivors: Is there a relationship between vascular reactivity and cardiorespiratory fitness?","authors":"Guilherme Dionir Back, Murilo Rezende Oliveira, Guilherme Peixoto Tinoco Arêas, Patricia Faria Camargo, Cássia da Luz Goulart, Claudio Ricardo de Oliveira, José Carlos Bonjorno Junior, Flávia Rossi Caruso Bonjourno, Ross Arena, Audrey Borghi-Silva","doi":"10.14814/phy2.70216","DOIUrl":"10.14814/phy2.70216","url":null,"abstract":"The impact of COVID-19 on cardiorespiratory fitness (CRF) is negative, increasing the likelihood of exertional symptoms such as fatigue and shortness of breath, and adversely affecting vascular function, impairing cardiovascular health. This study investigated endothelial function and its relationship with CRF in patients who have recovered COVID-19. Patients were evaluated 1 month after infection, including clinical assessment, pulmonary function, endothelial function (measured by flow-mediated dilation), and cardiopulmonary exercise testing. COVID-19 survivors exhibited reduced exercise capacity, with poor values of peak V̇O2peak and FMD (%) according to disease severity. However, endothelial function was worse in COVID-19 patients, regardless of severity, compared to the control group. Significant associations were observed between poorer FMD (%) and peak V̇O2, workload, circulatory power, and V̇O2peak/WR. Endothelial function was significantly associated with CRF in COVID-19 patients according to disease severity. Strategies to improve CRF and reduce the negative impacts of endothelial function damage should be further investigated.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 5","pages":"e70216"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute sympathetic activation blunts the hyperemic and vasodilatory response to passive leg movement in young healthy males.

IF 2.2

Physiological Reports Pub Date : 2025-03-01 DOI: 10.14814/phy2.70180

Brady E Hanson, Joshua F Lee, Ryan S Garten, Zachary Barrett-O'Keefe, Gwenael Layec, Bradley A Ruple, D Walter Wray, Russell S Richardson, Joel D Trinity

{"title":"Acute sympathetic activation blunts the hyperemic and vasodilatory response to passive leg movement in young healthy males.","authors":"Brady E Hanson, Joshua F Lee, Ryan S Garten, Zachary Barrett-O'Keefe, Gwenael Layec, Bradley A Ruple, D Walter Wray, Russell S Richardson, Joel D Trinity","doi":"10.14814/phy2.70180","DOIUrl":"10.14814/phy2.70180","url":null,"abstract":"Heightened muscle sympathetic nerve activity (MSNA) contributes to impaired vasodilatory capacity and vascular dysfunction associated with aging and cardiovascular disease. The contribution of elevated MSNA to the vasodilatory response during passive leg movement (PLM) is not fully understood. This study tested the hypothesis that elevated MSNA diminishes the vasodilatory response to PLM in healthy young males (n = 11, 25 ± 2 yr). Post exercise circulatory occlusion (PECO) following 2 min of isometric handgrip (HG) exercise performed at 25% (ExPECO 25%) and 40% (ExPECO 40%) maximum voluntary contraction was used to incrementally engage the metaboreceptors and augment MSNA. Control trials were performed without PECO (ExCON 25% and ExCON 40%) to account for changes due to HG exercise. PLM was performed 2 min after exercise and hemodynamics were assessed. MSNA was recorded by microneurography in the peroneal nerve (n = 8). Measures of MSNA (i.e., burst incidences) increased during ExPECO 25% (+15 ± 5 burst/100 bpm) and ExPECO 40% (+22 ± 4 burst/100 bpm) and returned to pre-HG levels during ExCON trials. Leg vascular conductance (vasodilation) during PLM was reduced by 16% and 44% during ExPECO 25% and ExPECO 40%, respectively. These findings indicate elevated MSNA attenuates the vasodilatory response to PLM and the magnitude of reduction in vasodilation during PLM is graded with the degree of sympathoexcitation.","PeriodicalId":20083,"journal":{"name":"Physiological Reports","volume":"13 5","pages":"e70180"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0