Plant Molecular Biology最新文献

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OsFPN1 functions as an essential metal transporter for Fe homeostasis and Co/Ni tolerance in rice. OsFPN1是水稻铁稳态和Co/Ni耐受性的重要金属转运体。
IF 3.7 2区 生物学
Plant Molecular Biology Pub Date : 2026-03-13 DOI: 10.1007/s11103-026-01701-1
Jindong Wu, Hiromi Nakanishi
{"title":"OsFPN1 functions as an essential metal transporter for Fe homeostasis and Co/Ni tolerance in rice.","authors":"Jindong Wu, Hiromi Nakanishi","doi":"10.1007/s11103-026-01701-1","DOIUrl":"10.1007/s11103-026-01701-1","url":null,"abstract":"<p><p>Iron (Fe) homeostasis in rice (Oryza sativa) requires coordinated uptake and root-to-shoot distribution and can be perturbed by competing transition metals such as cobalt (Co) and nickel (Ni). This study investigated the roles of the metal transporter OsFPN1 in Fe, Co, and Ni transport. OsFPN1–sGFP localized predominantly to the Golgi apparatus. In yeast, OsFPN1 conferred phenotypes consistent with Fe/Co/Ni transport activity. Seedlings carrying CRISPR/Cas9-induced knockout mutations in OsFPN1 were hypersensitive to iron deficiency (–Fe) and Co/Ni stress; under –Fe supplemented with 100 µM Ni, seedlings exhibited severe growth inhibition and loss of viability by day 10. Mutant plants accumulated less Fe in shoots but more in roots under –Fe, while Co and Ni treatments resulted in reduced root accumulation and increased shoot translocation. This implies impaired root retention and enhanced long-distance transport of Co and Ni in the mutants. Yeast two-hybrid (Y2H) screening identified 58 non-redundant candidates as OsFPN1-interacting proteins, with interactions of OsPAR1 (a PRA1-family vesicle trafficking factor) and Os12g0168900 (a vacuolar V-ATPase subunit) validated by Y2H and bimolecular fluorescence complementation (BiFC). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed elevated expression of OsIMA1, OsIMA2, OsPAR1, and Os12g0168900 in mutant shoots, and stronger induction of OsIMA1, OsIMA2, OsNAS2, OsPAR1, and Os12g0168900 in roots specifically under –Fe. These results show that Golgi-localized OsFPN1 is required for proper root-to-shoot Fe allocation and contributes to limiting Co/Ni translocation under metal stress, while the identified protein associations provide candidate links to endomembrane/vacuolar pathways for future functional investigation.</p>","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12987785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147459294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome wide analysis of RLK-Pelle-L-LEC family in Rosa chinensis and negative regulation of black spot disease by RcPLLEC45. 月季RLK-Pelle-L-LEC家族全基因组分析及RcPLLEC45对黑斑病的负调控
IF 3.7 2区 生物学
Plant Molecular Biology Pub Date : 2026-03-12 DOI: 10.1007/s11103-026-01694-x
Xingyu Liu, Xiaowen Zheng, Haijiao Wang, Tingliang Xu
{"title":"Genome wide analysis of RLK-Pelle-L-LEC family in Rosa chinensis and negative regulation of black spot disease by RcPLLEC45.","authors":"Xingyu Liu, Xiaowen Zheng, Haijiao Wang, Tingliang Xu","doi":"10.1007/s11103-026-01694-x","DOIUrl":"10.1007/s11103-026-01694-x","url":null,"abstract":"<p><p>Protein kinases represent one of the largest protein families in plants and play vital roles in growth, development, and stress responses. In this study, we identified 78 RLK-Pelle-L-LEC (PLLEC) subfamily members within the Rosa chinensis genome. Through comprehensive analyses, including genome-wide identification, collinearity assessment, examination of promoter cis-regulatory elements, and gene expression profiling, we elucidated the structural and functional diversity of the RcPLLEC subfamily. Evolutionary analysis indicated that these 78 RcPLLEC genes are largely conserved, with evidence of tandem duplication events contributing to their expansion. Cis-acting element analysis within promoter regions, combined with gene expression clustering, revealed that most RcPLLEC family members possess light-responsive elements and regulatory motifs associated with environmental stress. Furthermore, infection by the fungal pathogen Marssonina rosae (M. rosae) significantly perturbed the expression of the majority of RcPLLEC genes. Notably, the expression profile of the RcPLLEC45 gene exhibited a distinct bimodal pattern during infection. Utilizing Virus-Induced Gene Silencing (VIGS), we investigated the functional role of RcPLLEC45 during M. rosae infection, specifically monitoring alterations in hormone levels and relevant physiological indicators. Our findings demonstrate that RcPLLEC45 expression negatively regulates defense responses against black spot disease. Moreover, we provide evidence suggesting that RcPLLEC45 expression potentially facilitates jasmonic acid (JA) biosynthesis, modulates catalase activity, and influences hemicellulose synthesis, collectively contributing to the susceptibility of rose to M. rosae infection.</p>","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147444565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid selection of early-maturing and low-ear position maize hybrids suitable for China using genomic prediction. 利用基因组预测快速筛选适合中国的早熟低穗玉米杂交品种。
IF 3.7 2区 生物学
Plant Molecular Biology Pub Date : 2026-03-05 DOI: 10.1007/s11103-026-01693-y
Ping Luo, Wenzhe Li, Lin Zhang, Jie Yang, Houwen Wang, Fei Wang, Ruisi Yang, Xinlong Xu, Ke Yang, Hongjun Yong, Mingshun Li, Jianfeng Weng, Degui Zhang, Zhiqiang Zhou, Jienan Han, Wenwei Gao, Junjie Fu, Xinhai Li, Zhiyong Ni, Zhuanfang Hao
{"title":"Rapid selection of early-maturing and low-ear position maize hybrids suitable for China using genomic prediction.","authors":"Ping Luo, Wenzhe Li, Lin Zhang, Jie Yang, Houwen Wang, Fei Wang, Ruisi Yang, Xinlong Xu, Ke Yang, Hongjun Yong, Mingshun Li, Jianfeng Weng, Degui Zhang, Zhiqiang Zhou, Jienan Han, Wenwei Gao, Junjie Fu, Xinhai Li, Zhiyong Ni, Zhuanfang Hao","doi":"10.1007/s11103-026-01693-y","DOIUrl":"10.1007/s11103-026-01693-y","url":null,"abstract":"<p><p>Earlymaturity and lowear position traits are important selective traits in maize breeding procedures, especially in northern China. To construct a genomic prediction model for selecting early-maturity and low-ear-position hybrids adapted to different environments, 34elite inbred lines were selected to produce 285 single-cross hybrids using a partial diallel cross design. These inbred lines constituted a mini-core collection of Chinese maize germplasm, comprising 18 inbred lines from the Stiff Stalk heterotic group and 16 inbred lines from the Non-Stiff Stalk heterotic group. The parental inbred lines were genotyped by sequencing, and the 285 hybrids were phenotyped for early-maturity and low-ear-position-related traits at seven locations in China. The results revealed significant variations in early maturity and low ear position traits among seven locations. These traits were highly correlated across locations, with high heritability ranging from 0.89 to 0.94, indicating that genetic factors primarily govern early maturity and ear position. The population structure showed that the hybrids were mainly composed of four subgroups. Among them, the Reid × Iodent subgroup exhibited the shortest growth period and the lowest ear position across the seven locations and showed relatively high genetic diversity. Correspondingly, the low general combining abilities of the inbred lines in both subgroups across the locations were also identified. The genomic prediction accuracy of these agronomic traits ranged from 0.26 to 0.89. Accuracy across seven locations ranged from 0.37 to 0.89. We also found that effective genomic prediction models can be set and developed using a training set comprising less than 16% of the entire dataset, covering both breeding aims. Genomic prediction achieved moderate to high accuracy with a small training set, demonstrating clear potential to streamline hybrid selection and enhance prediction stability.</p>","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential activity of CYTOKININ OXIDASE/DEHYDROGENASE3 (CKX3) and CKX5 genes in regulating yield components in Brassica napus L. 细胞分裂素氧化酶/脱氢酶e3 (CKX3)和CKX5基因在调控甘蓝型油菜产量成分中的差异活性
IF 3.7 2区 生物学
Plant Molecular Biology Pub Date : 2026-03-05 DOI: 10.1007/s11103-025-01678-3
Ireen Schwarz, Christian Möllers, Thomas Schmülling
{"title":"Differential activity of CYTOKININ OXIDASE/DEHYDROGENASE3 (CKX3) and CKX5 genes in regulating yield components in Brassica napus L.","authors":"Ireen Schwarz, Christian Möllers, Thomas Schmülling","doi":"10.1007/s11103-025-01678-3","DOIUrl":"10.1007/s11103-025-01678-3","url":null,"abstract":"<p><p>Cytokinin is a plant hormone that regulates several yield-related traits in plants. Previously, it was demonstrated that in tetraploid oilseed rape (Brassica napus L.), mutation of all four cytokinin-degrading BnCKX3 and both BnCKX5 genes resulted in increased cytokinin concentration, larger and more active inflorescence meristems, and a higher number of ovules per gynoecium. This resulted in the formation of more flowers and pods on the main stem, thereby increasing seed yield from the main stem of the plants. Here, we investigated the relative contributions of distinct combinations of BnCKX3 and BnCKX5 genes of the A and C genomes to these yield components. Our analysis revealed an unexpectedly strong role for BnCKX5 in regulating these traits and identified distinct supportive BnCKX3 gene mutant combinations. These findings facilitate the selection of relevant alleles for breeding. Furthermore, seeds from BnCKX gene mutant plants showed oil content and concentrations of unsaturated fatty acids similar to those of the wild type. Taken together, this study provides further insight into the role of cytokinin and BnCKX genes in regulating yield components in oilseed rape and provides novel information on functionally relevant alleles.</p>","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12963132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome-wide association mapping, phylogenetic study, and multivariate analysis of component traits of grain yield in wheat under heat stress conditions. 热胁迫条件下小麦产量组成性状的全基因组关联图谱、系统发育研究及多变量分析。
IF 3.7 2区 生物学
Plant Molecular Biology Pub Date : 2026-03-05 DOI: 10.1007/s11103-026-01695-w
Ezhumalai Sivapragasam, Sukumar Taria, Sudhir Kumar, Hari Krishna, Narayana Bhat Devate, Biswabiplab Singh, Elangovan Allimuthu, Dhandapani Raju, Ranjit Ranjan Kumar, Rabi Narayan Sahoo, Monika Dalal, Renu Pandey, Viswanathan Chinnusamy
{"title":"Genome-wide association mapping, phylogenetic study, and multivariate analysis of component traits of grain yield in wheat under heat stress conditions.","authors":"Ezhumalai Sivapragasam, Sukumar Taria, Sudhir Kumar, Hari Krishna, Narayana Bhat Devate, Biswabiplab Singh, Elangovan Allimuthu, Dhandapani Raju, Ranjit Ranjan Kumar, Rabi Narayan Sahoo, Monika Dalal, Renu Pandey, Viswanathan Chinnusamy","doi":"10.1007/s11103-026-01695-w","DOIUrl":"10.1007/s11103-026-01695-w","url":null,"abstract":"<p><p>Heat stress negatively impacts key yield-contributing physiological traits in wheat, leading to a decrease in grain yield. Scanning of genomic regions linked to these traits, along with the identification of the most relevant candidate genes (CGs), is an effective strategy for developing heat-tolerant wheat cultivars in the near future. In this context, a genome-wide association mapping approach has been employed to identify chromosomal regions associated with these traits, along with to identify the putative CGs for heat tolerance in wheat. Genotyping was performed using the 35 K Axiom Wheat Breeder Array. From our study, principal component analysis (PCA) revealed that biomass (BM), canopy temperature (CT), and seed weight per pot (SWPP) explained a higher cumulative variance. Population structure and diversity analysis filtered 13,947 markers and revealed three subpopulations with sufficient diversity. A large whole-genome LD block size of 7.15 MB was obtained at a half LD decay value. We have mapped 14 significant MTAs linked to these traits with - log10(p) value > 5.44 after Bonferroni correction and also identified 14 high-confidence CGs. Our study also identified four haplotype groups, suggesting the potential for a haplotype-based breeding program under heat stress. Promoter analysis revealed 174 cis-regulatory elements (CREs). Phylogenetic analysis of the pleiotropic gene TraesCS7A02G200200 revealed three major clades of closely related species. We have also reported several orthologous genes related to our 14 major CGs. Untranslated regions (UTRs) analysis found several upstream Open Reading Frames (uORFs) in few identified genes, which can be employed to understand the stringent mechanism of gene regulation under heat stress. By using the Multitrait-genotype ideotype index (MGIDI), we have selected 13 high-performance genotypes for their use as donor parent for heat tolerance. Henceforth, after successful validation, these SNPs can be utilized for marker-assisted transfer of genes/QTLs to develop heat-tolerant wheat cultivars.</p>","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rhizobial Nod factors modulate reactive oxygen species, jasmonates, and pattern-recognizing receptors to suppress immune response. 根瘤菌Nod因子调节活性氧、茉莉酸盐和模式识别受体来抑制免疫反应。
IF 3.7 2区 生物学
Plant Molecular Biology Pub Date : 2026-03-05 DOI: 10.1007/s11103-026-01692-z
A M Dymo, P Yu Kozyulina, A V Dolgikh, E S Kantsurova, K V Danko, O A Pavlova, T S Leonova, A D Bovin, N V Smirnova, P A Kulesh, A A Frolov, E A Dolgikh
{"title":"Rhizobial Nod factors modulate reactive oxygen species, jasmonates, and pattern-recognizing receptors to suppress immune response.","authors":"A M Dymo, P Yu Kozyulina, A V Dolgikh, E S Kantsurova, K V Danko, O A Pavlova, T S Leonova, A D Bovin, N V Smirnova, P A Kulesh, A A Frolov, E A Dolgikh","doi":"10.1007/s11103-026-01692-z","DOIUrl":"10.1007/s11103-026-01692-z","url":null,"abstract":"<p><p>Fine-tuning of the immune response plays a key role in legume-rhizobial symbiosis. Rhizobial Nod factors can suppress the defense responses during symbiosis, but the possible mechanisms of such regulation remain poorly understood. Here, we observe that Nod factors effectively suppress the expression of genes encoding defense markers (WRKYs, PRs, PALs), the reactive oxygen species (ROS) formation, and reduce the content of pattern recognition receptor (PRR) LYK9 induced by treatment with deacetylated chitooligosaccharide CO8-DA in pea roots. Since PRR LYK9 may recognize both chitin/COs and peptidoglycan, it likely plays an important role in the activation of defense responses during rhizobial inoculation. To identify potential regulators through which Nod factors suppress the immune response in plants during symbiosis with rhizobia, proteome and transcriptome analyses were performed. This allowed identifying several potential candidates activated by Nod factors, such as superoxide dismutase and catalase enzymes, which prevent excessive ROS accumulation and the development of oxidative stress. We also found ubiquitin ligases and ubiquitin-conjugating enzymes that may target PRRs activated in response to rhizobial inoculation. LYK9 degradation via ubiquitinylation was shown to prevent a hypersensitive response in plants. Nod factors activate enzymes involved in jasmonic acid biosynthesis, which in turn activates the transcription factor ABR1, suppressing the abscisic acid-induced responses and decreasing the immune response. Finally, we showed that LysM-receptor-like kinases PsLYK11/MtLYK11, probable homologs of Arabidopsis AtLYK3 in pea and Medicago, are involved in regulation of the immune response.</p>","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Discovery of the loci associated with the test weight and capsule seed weight variation in Sesame (Sesamum indicum L.). 芝麻(Sesamum indicum L.)试重和蒴果重变异相关基因座的发现。
IF 3.7 2区 生物学
Plant Molecular Biology Pub Date : 2026-02-26 DOI: 10.1007/s11103-026-01686-x
Desawi Hdru Teklu, Jun You, Yuan Gao, Rong Zhou, Muez Berhe, Ahmed A Abbas, Yanxin Zhang, Linhai Wang
{"title":"Discovery of the loci associated with the test weight and capsule seed weight variation in Sesame (Sesamum indicum L.).","authors":"Desawi Hdru Teklu, Jun You, Yuan Gao, Rong Zhou, Muez Berhe, Ahmed A Abbas, Yanxin Zhang, Linhai Wang","doi":"10.1007/s11103-026-01686-x","DOIUrl":"10.1007/s11103-026-01686-x","url":null,"abstract":"<p><p>Genetic improvement on the economical yield-related traits of sesame is crucial for desirable trait selection for analysis. Hence, understanding the genetic basis of genes regulating test weight and capsule seed weight traits and its association with seed yield is crucial to guide breeding program. The objective of the study was to understand natural variations and discover the genetic markers and key genes regulating test weight (TW) and capsule seed weight (CSW) in sesame. 401 diverse sesame genotypes were evaluated under four and two environments for test weight and capsule seed weight, respectively. Results revealed diverse genetic variations on studied traits cross environments. The total TW ranged from 496.23 to 672.92 g L<sup>- 1</sup>, 436.15 to 678.20 g L<sup>- 1</sup> 478.79 to 672.92 g L<sup>- 1</sup>, and 450.40 to 682.61 g L<sup>- 1</sup> in 2023Wuhan, 2019 Jinxian, 2022Wuhan, and 2019Zhumadian environments, respectively. Whereas, CSW varied from 0.02 to 0.29 g in 2023Linqing and 0.05 to 0.30 g in 2023Wuhan. The GWAS detected four (3 SNPs and Indel) and two (1 SNP and InDel) significant SNP and InDel loci associated with TW and CSW, respectively. Of the SNPs, SNP11:142842 was more stable and associated with TW across the four environments. Moreover, SNP7:5945502, and InDel10:18621286 were identified for CSW in Linqing and Wuhan environments, respectively. Candidate genes SINPZ1100015, SINPZ0700254 and SINPZ1001450 were associated with TW and CSW and may represent the major candidate effect gene for the studied traits in sesame. Our findings provide insights into natural variation in sesame for TW and CSW and valuable information for markers-assisted breeding in sesame and other crops.</p>","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147290678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: Uncovering the molecular basis of high morphinan product efficiency in opium poppy through Multi-omics integrated analysis with multi-capsules. 更正:通过多胶囊的多组学综合分析揭示了罂粟中吗啡肽高产物效率的分子基础。
IF 3.8 2区 生物学
Plant Molecular Biology Pub Date : 2026-02-26 DOI: 10.1007/s11103-026-01697-8
Yumin Huang, Mao Sun, Wuhu Gong, Yifeng Liu, Yimeng Cheng, Lijuan Yuan, Yuanming Wu
{"title":"Correction: Uncovering the molecular basis of high morphinan product efficiency in opium poppy through Multi-omics integrated analysis with multi-capsules.","authors":"Yumin Huang, Mao Sun, Wuhu Gong, Yifeng Liu, Yimeng Cheng, Lijuan Yuan, Yuanming Wu","doi":"10.1007/s11103-026-01697-8","DOIUrl":"10.1007/s11103-026-01697-8","url":null,"abstract":"","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147290659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A cyclic nucleotide-gated channel gene HcCNGC11 positively regulates salt stress responses in kenaf (Hibiscus cannabinus L.). 环核苷酸门控通道基因HcCNGC11正调控红麻(Hibiscus cannabinus L.)的盐胁迫反应。
IF 3.7 2区 生物学
Plant Molecular Biology Pub Date : 2026-02-25 DOI: 10.1007/s11103-026-01696-9
Jiao Yue, Canni Chen, Qiuping Wang, Rehmat Ullah, Yuqi Tan, Xu Wang, Huaming Lu, Dengjie Luo, Ru Li, Tao Chen, Peng Chen
{"title":"A cyclic nucleotide-gated channel gene HcCNGC11 positively regulates salt stress responses in kenaf (Hibiscus cannabinus L.).","authors":"Jiao Yue, Canni Chen, Qiuping Wang, Rehmat Ullah, Yuqi Tan, Xu Wang, Huaming Lu, Dengjie Luo, Ru Li, Tao Chen, Peng Chen","doi":"10.1007/s11103-026-01696-9","DOIUrl":"10.1007/s11103-026-01696-9","url":null,"abstract":"<p><p>Cyclic nucleotide-gated channels (CNGCs) are evolutionarily conserved calcium-permeable non-selective cation channels that play critical regulatory roles in plant abiotic stress responses. This study characterizes HcCNGC11 in kenaf (Hibiscus cannabinus L.) through integrated genomic and functional analyses. Subcellular localization analysis using GFP-fusion constructs confirmed plasma membrane-specific targeting of HcCNGC11. Tissue-specific expression profiling revealed that HcCNGC11 transcripts accumulate predominantly in roots (2.8-fold higher than leaves), followed by leaves, stems, flowers, and seeds. Notably, HcCNGC11 demonstrated rapid transcriptional upregulation under 150 mM NaCl stress, reaching maximum induction at 3 h post-treatment. Virus-induced gene silencing of HcCNGC11 significantly inhibited kenaf growth under salt stress. Biochemical analyses of the silenced lines showed 5-66% reduced activities of antioxidant enzymes (SOD, POD, CAT), decreased osmoregulatory substances (soluble protein, proline), reduced chlorophyll content, and elevated ROS (H₂O₂, O₂·⁻) accumulation. Under salt stress, HcCNGC11-silenced plants displayed significant downregulation of antioxidant enzyme genes (HcSOD, HcPOD, HcCAT) as well as stress-responsive genes (HcP5CS, HcLTP, HcNCED). Conversely, Arabidopsis lines overexpressing HcCNGC11 exhibited 20-47% higher antioxidant enzyme activities, increased osmoregulatory substances, enhanced chlorophyll content, and markedly reduced ROS accumulation compared to WT under salt stress. Molecular analysis of these transgenic lines showed upregulated expression of antioxidant genes (AtSOD1, AtPOD1, AtCAT1) and stress-responsive genes (AtSOS1, AtNHX1, AtCOR15). Protein-protein interaction studies, employing both yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays, identified multiple HcCNGC11 binding partners, including HcCaM7, HcCNGC21, HcTHI1, and HcTCP14. Collectively, these results demonstrate that HcCNGC11 positively regulates plant salt tolerance through modulation of antioxidant systems and stress-responsive pathways.</p>","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147284707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of an (E, E)-farnesyl pyrophosphate synthase in Saxifraga stolonifera involved in triterpenoid biosynthesis. 三萜类生物合成中一种(E, E)-法尼酯焦磷酸合成酶的研究。
IF 3.7 2区 生物学
Plant Molecular Biology Pub Date : 2026-02-22 DOI: 10.1007/s11103-026-01687-w
Chengjia Zhao, Jing Han, Shunbao Huang, Xueping Su, Li Cai, Hualei Wang, Jin Liang
{"title":"Characterization of an (E, E)-farnesyl pyrophosphate synthase in Saxifraga stolonifera involved in triterpenoid biosynthesis.","authors":"Chengjia Zhao, Jing Han, Shunbao Huang, Xueping Su, Li Cai, Hualei Wang, Jin Liang","doi":"10.1007/s11103-026-01687-w","DOIUrl":"10.1007/s11103-026-01687-w","url":null,"abstract":"<p><p>Saxifraga stolonifera is a Chinese traditional medicine for otitis media treatment. A few terpenoids were identified from S. stolonifera but the involved genes are unclear. Here, we mined a farnesyl pyrophosphate synthase gene (SsFPS) from the full-length transcriptome of S. stolonifera and confirmed it was expressed in cytoplasm. Subsequently, the enzymatic activity of SsFPS was characterized by in-vitro assays and heterologous expression in E.coli. Moreover, the gene expression pattern of SsFPS was positively correlated to which of a squalene synthase gene (SsSS) involved in triterpenoid biosynthesis and consistent to the accumulation pattern of total saponins in tissues. Taken together, SsFPS was characterized as an (E, E)-farnesyl pyrophosphate synthase in S. stolonifera and suggested to participate in sesquiterpenoid and triterpenoid biosynthesis which provides the support to explore the biosynthesis and pharmacological activity of triterpenoid in Saxifraga.</p>","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147271514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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