Plant Molecular Biology最新文献_第8页

Comparative transcriptomic analysis of heterotic maize development during kernel filling. 杂种玉米灌浆期发育的比较转录组学分析。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-04-02 DOI: 10.1007/s11103-025-01584-8

Wenyu Li, Xiangkun Guo, Wen Yao, Keke Li, Qi Zheng, Yongbiao Yu, Zhiwei Zhang, Yan Wang, Weigang Yao, Ju Wu, Huan Hu, Lingwei Hu, Long Zhang, Xinyu Li, Yongbin Dong, Yuling Li

{"title":"Comparative transcriptomic analysis of heterotic maize development during kernel filling.","authors":"Wenyu Li, Xiangkun Guo, Wen Yao, Keke Li, Qi Zheng, Yongbiao Yu, Zhiwei Zhang, Yan Wang, Weigang Yao, Ju Wu, Huan Hu, Lingwei Hu, Long Zhang, Xinyu Li, Yongbin Dong, Yuling Li","doi":"10.1007/s11103-025-01584-8","DOIUrl":"10.1007/s11103-025-01584-8","url":null,"abstract":"Heterosis, characterized by enhanced performance of a hybrid relative to its parental lines, has been a fundament of plant breeding strategies. Despite the application of heterosis, its molecular mechanisms remain elusive. Here, we focused on the maize heterotic hybrid Yudan132, which showed enhanced agronomic traits compared to its parental lines, including ear and kernel size, kernel weight, and overall yield. Notably, Yudan132 showed increased accumulation of storage substances, characterized by starch, protein contents and grain-filling rates, all of which collectively contribute to the augmented kernel weight. Through gene expression profiling, we identified differentially expressed genes (DEGs) in Yudan132 and its parental lines across four distinct kernel developmental stages (12, 20, 28, and 40 days after pollination). These DEGs displayed both additive and non-additive expression patterns, each contributing to heterosis in maize kernels. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis highlighted their involvement in metabolic pathways, biosynthesis of secondary metabolites, carbon metabolism, starch and sucrose metabolism processes. Within these pathways, the enriched DEGs predominantly associated with the gene categories of peroxidase, cytochrome P450, ketoacyl-CoA synthase, and phospholipase D. Furthermore, we identified the transcription factor bZIP88 among the DEGs, which was involved in the regulation of seed size and weight in transgenic Arabidopsis. These results suggested a potential role for bZIP88 in modulating kernel development, thereby further implicating the involvement of the identified DEGs in the molecular mechanisms of heterosis. These findings provide the genetic role of heterosis in kernel and the molecular mechanism regulating kernel development.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"53"},"PeriodicalIF":3.9,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simulation study of factors affecting the accuracy of transcriptome models under complex environments. 复杂环境下影响转录组模型准确性因素的模拟研究。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-28 DOI: 10.1007/s11103-025-01578-6

Dan Eiju, Yoichi Hashida, Taro Maeda, Koji Iwayama, Atsushi J Nagano

{"title":"Simulation study of factors affecting the accuracy of transcriptome models under complex environments.","authors":"Dan Eiju, Yoichi Hashida, Taro Maeda, Koji Iwayama, Atsushi J Nagano","doi":"10.1007/s11103-025-01578-6","DOIUrl":"10.1007/s11103-025-01578-6","url":null,"abstract":"Characterization of molecular responses in real and complex field environments is essential for understanding the environmental response of plants. Field transcriptomics prediction consists of modeling of transcriptomes in outdoor fields with various environmental variables: Meteorological parameters, atmospheric gases, soil conditions, herbivores, management, etc. It is the most comprehensive method of studying gene expression dynamics in complex environments. However, it is not clear what factors influence the accuracy of field transcriptome models. In this study, a novel simulation system was developed. Using the system, we performed a large-scale simulation to reveal the factors affecting the accuracy of the models. We found that the factors that had the greatest impact on the accuracy are, in order of importance, the expression pattern of the gene, the number of samples in the training data, the diurnal coverage of the training data, and the temperature coverage of the training data. Validation using actually measured transcriptome data showed similar results to the simulations. Our simulation system and the analysis results will be helpful for developing efficient sampling strategies for training data and for generating simulated data for benchmarking new modelling methods. It will also be valuable to dissect the relative importance of various factors behind transcriptome dynamics in the real environment.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"52"},"PeriodicalIF":3.9,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological and compensatory roles of three starch-branching enzymes in different rice organs. 三种淀粉分支酶在水稻不同器官中的生理和代偿作用。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-26 DOI: 10.1007/s11103-025-01573-x

Satoko Miura, Naoko Crofts, Nana Koyama, Yuko Hosaka, Ryutaro Morita, Misato Abe, Yasunori Nakamura, Naoko Fujita

{"title":"Physiological and compensatory roles of three starch-branching enzymes in different rice organs.","authors":"Satoko Miura, Naoko Crofts, Nana Koyama, Yuko Hosaka, Ryutaro Morita, Misato Abe, Yasunori Nakamura, Naoko Fujita","doi":"10.1007/s11103-025-01573-x","DOIUrl":"10.1007/s11103-025-01573-x","url":null,"abstract":"Starch-branching enzymes (BEs) generate amylopectin branches in starch, and three isoforms are found in the rice genome. BEI and BEIIa are expressed ubiquitously, on the other hand BEIIb is exclusively expressed in the endosperm. The loss of BEIIb generates opaque seeds with drastically reduced short amylopectin chains, whereas the loss of BEI slightly reduces long amylopectin chains. However, the loss of BEIIa has no obvious effect on amylopectin structure in the endosperm. Little is known about these isozymes' specific and compensatory roles in other tissues. In this study, all three combinations of double mutants were generated, and their starch properties were analyzed to reveal the function of the solely remaining isozyme, deduce the compensatory function of the missing isozymes, and clarify their roles in different organs. be2a be2b displayed severe sterility and hindered starch synthesis in the endosperm; be1 be2a displayed only minor alterations in endosperm starch; but be1 be2b displayed a drastic increase in amylose content in the endosperm. These results indicate that BEIIa has some compensatory function for BEIIb, and BEI alone is insufficient. Additionally, BEIIa or BEIIb is necessary for endosperm formation. Unlike the endosperm, short amylopectin chains were drastically reduced in the leaf sheath of be1 be2a and be2a be2b, and amylose content was drastically increased in the leaf sheath of be1 be2a and be2a be2b but not be1 be2b. These results indicate that BEIIa has a major role in the leaf sheath, and the generation of short amylopectin chains by BEII can be partially compensated by BEI.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"51"},"PeriodicalIF":3.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143721003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidating the antioxidant potential of some flavanones as MAO-B inhibitors through DAM, in silico molecular docking and computational analysis. 通过DAM、硅学分子对接和计算分析，阐明一些黄酮类化合物作为MAO-B抑制剂的抗氧化潜力。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-24 DOI: 10.1007/s11103-025-01567-9

E Athira, S Akhila Darsan, Shinta Davis, Vijisha K Rajan

{"title":"Elucidating the antioxidant potential of some flavanones as MAO-B inhibitors through DAM, in silico molecular docking and computational analysis.","authors":"E Athira, S Akhila Darsan, Shinta Davis, Vijisha K Rajan","doi":"10.1007/s11103-025-01567-9","DOIUrl":"10.1007/s11103-025-01567-9","url":null,"abstract":"Seven flavanones underwent computational evaluation to determine their effectiveness in filtering UV radiation and scavenging free radicals. The investigated flavanones exhibited enhanced radical scavenging capabilities relative to the parent flavanone, with Hesperidin demonstrating the highest EA and Qmax values, consistent with its antireductant activity. The remaining flavanones displayed lower IE values, suggesting their antioxidant efficacy. Spectroscopic analysis revealed that the HOMO-LUMO and HOMO-1-LUMO transitions are the primary electronic transitions in the UV-Visible spectra of the studied flavanones. Their absorption within the UV-A and UV-B range (260-345 nm) indicates potential utility as UV filters. Theoretical calculations demonstrate that the reactivity of flavanones is concentrated in ring [B], with a reactivity order of 3' > 4' > 2' > 6 > 7 > 5. The BDE values reveal that the 3'-OH group has the lowest value, followed by the 4' position, while hydrogen bonding is responsible for the increased BDE value at position 5. The values of ΔBDE and ΔAIP, relative to phenol, provide a framework for elucidating the preferred mechanism, HAT or SET, underlying the antioxidant behavior. Molecular docking simulations identified hesperetin, 2'-Hydroxyflavanone, 4'-Hydroxyflavanone, Eriodictyol, and Naringenin as potential MAO-B inhibitors, outperforming their synthetic counterparts in this regard.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"50"},"PeriodicalIF":3.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of genes associated with soybean protein content using a genome-wide association study. 利用全基因组关联研究检测大豆蛋白含量相关基因。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-22 DOI: 10.1007/s11103-025-01576-8

Zhiyuan Yu, Bo Hu, Hailong Ning, Wen-Xia Li

{"title":"Detection of genes associated with soybean protein content using a genome-wide association study.","authors":"Zhiyuan Yu, Bo Hu, Hailong Ning, Wen-Xia Li","doi":"10.1007/s11103-025-01576-8","DOIUrl":"10.1007/s11103-025-01576-8","url":null,"abstract":"The protein content in soybean seeds serves as a crucial measure of soybean quality. Breeding high-protein varieties remains the most cost-effective and efficient approach to increasing soybean protein levels. Nevertheless, limited research has focused on identifying the genes responsible for high protein content among the diverse soybean cultivars. To address this gap, a genome-wide association study (GWAS) was conducted on 455 soybean varieties with varying protein content to predict and validate novel genes involved in regulating protein levels in soybean seeds. Protein content data were obtained from three distinct environments, along with three environmental variables derived from oil content, which is closely related to protein levels. Genotyping was performed using the SoySNP180k BeadChip, yielding genotype data for 63,306 non-redundant single nucleotide polymorphisms (SNPs). Five multi-locus GWAS methods were employed, resulting in the identification of 81 significant quantitative trait nucleotides (QTNs), of which 37 QTNs detected across different methods and environments were further analyzed. Moreover, the simulation platform Blib was used to conduct single-crossing simulation breeding on 81 QTN loci for actual breeding prediction. Haplotype analysis based on re-sequencing data confirmed 2 genes closely linked to protein synthesis, providing a theoretical basis for breeding high-protein soybean varieties and developing molecular breeding strategies.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"49"},"PeriodicalIF":3.9,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Arabidopsis F-box protein FBS associated with the helix-loop-helix transcription factor FAMA involved in stomatal immunity. 拟南芥F-box蛋白FBS与螺旋-环-螺旋转录因子FAMA相关，参与气孔免疫。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-20 DOI: 10.1007/s11103-025-01577-7

Chunxia Zhang, Junling Yue, Shi Li, Chaoran Zuo, Yi Li, Qixiumei He, Jie Le

引用次数: 0

Chemical application improves stress resilience in plants. 施用化学药剂可提高植物的抗逆性。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-19 DOI: 10.1007/s11103-025-01566-w

Khurram Bashir, Daisuke Todaka, Kaori Sako, Minoru Ueda, Farhan Aziz, Motoaki Seki

{"title":"Chemical application improves stress resilience in plants.","authors":"Khurram Bashir, Daisuke Todaka, Kaori Sako, Minoru Ueda, Farhan Aziz, Motoaki Seki","doi":"10.1007/s11103-025-01566-w","DOIUrl":"10.1007/s11103-025-01566-w","url":null,"abstract":"In recent years, abiotic stresses, including droughts, floods, high temperatures, and salinity, have become increasingly frequent and severe. These stresses significantly hinder crop yields and product quality, posing substantial challenges to sustainable agriculture and global food security. Simultaneously, the rapidly growing global population exacerbates the need to enhance crop production under worsening environmental conditions. Consequently, the development of effective strategies to strengthen the resilience of crop plants against high temperatures, water scarcity, and extreme environmental conditions is critical for mitigating the impacts of abiotic stress. Plants respond to these environmental challenges by reprogramming their transcriptome and metabolome. Common strategies for developing stress-tolerant plants include screening germplasm, generating transgenic crop plants, and employing genome editing techniques. Recently, chemical treatment has emerged as a promising approach to enhance abiotic stress tolerance in crops. This technique involves the application of exogenous chemical compounds that induce molecular and physiological changes, thereby providing a protective shield against abiotic stress. Forward and reverse genetic approaches have facilitated the identification of chemicals capable of modulating plant responses to abiotic stresses. These priming agents function as epigenetic regulators, agonists, or antagonists, playing essential roles in regulating stomatal closure to conserve water, managing cellular signaling through reactive oxygen species and metabolites to sustain plant growth, and activating gluconeogenesis to enhance cellular metabolism. This review summarizes recent advancements in the field of chemical priming and explores strategies to improve stress tolerance and crop productivity, thereby contributing to the enhancement of global food security.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"47"},"PeriodicalIF":3.9,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11922999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemically-induced cellular stress signals are transmitted to alternative splicing via UsnRNA levels to alter gene expression in Arabidopsis thaliana. 化学诱导的细胞应激信号通过UsnRNA水平传递到选择性剪接，从而改变拟南芥的基因表达。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-16 DOI: 10.1007/s11103-025-01575-9

Hirokazu Takahashi, Toshihiro Arae, Kodai Ishibashi, Ryosuke Sano, Taku Demura, Misato Ohtani

{"title":"Chemically-induced cellular stress signals are transmitted to alternative splicing via UsnRNA levels to alter gene expression in Arabidopsis thaliana.","authors":"Hirokazu Takahashi, Toshihiro Arae, Kodai Ishibashi, Ryosuke Sano, Taku Demura, Misato Ohtani","doi":"10.1007/s11103-025-01575-9","DOIUrl":"10.1007/s11103-025-01575-9","url":null,"abstract":"Alternative pre-mRNA splicing (AS) is a crucial regulatory layer of gene expression in eukaryotes. AS patterns can change in response to abiotic and biotic stress, allowing cellular functions to adapt to environmental conditions. Here, we examined the effects of cellular stress-inducing chemicals on AS-mediated gene regulation in Arabidopsis thaliana by investigating the alternatively spliced forms of SERINE-ARGININE PROTEIN30 (SRp30) and U1-70 K, encoding splicing factors, as well as ASCORBATE PEROXIDASE3 (APX3) and FOLYLPOLYGLUTAMATE SYNTHASE3 (FPGS3), encoding enzymes important for stress responses. Disrupting key cellular activities, including nitric oxide metabolism, ATPase activity, plastid function, and genome stability, affected AS patterns in Arabidopsis. Stress treatment altered the abundance of uridine-rich small nuclear RNAs (UsnRNAs), especially U1 snRNAs, which are essential non-coding RNA components of U1 small nuclear ribonucleoproteins (U1 snRNPs), suggesting that abnormalities in AS are partially mediated by changes in U1 snRNA levels. The shoot redifferentiation defectice2-1 (srd2-1) mutant defective for snRNA transcription was hypersensitive for stress treatment, since it showed changes in AS patterns at lower concentrations of stress inducers to compare with the wild type. Together, our data suggest that cellular stress can influence gene expression in plants by regulating AS, which is partially regulated by UsnRNA levels through the SRD2-mediated snRNA transcription.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"46"},"PeriodicalIF":3.9,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A glycogen synthase kinase-3 gene enhances grain yield heterosis in semi-dwarf rapeseed. 糖原合酶激酶-3基因可提高半矮油菜籽的籽粒产量异质性。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-14 DOI: 10.1007/s11103-025-01555-z

Li Bao, Liu Xinhong, Yang Qian, Zhang Hui, Tan Wenqing, Yan Mingli, Deng Lichao, Li Mei, Qu Liang, Guo Yiming

{"title":"A glycogen synthase kinase-3 gene enhances grain yield heterosis in semi-dwarf rapeseed.","authors":"Li Bao, Liu Xinhong, Yang Qian, Zhang Hui, Tan Wenqing, Yan Mingli, Deng Lichao, Li Mei, Qu Liang, Guo Yiming","doi":"10.1007/s11103-025-01555-z","DOIUrl":"10.1007/s11103-025-01555-z","url":null,"abstract":"Optimizing plant height is a key breeding objective in Brassica napus to enhance lodging resistance and increase yield potential. In the present study, we identified a semi-dwarf gene in rapeseed, BnDWARF5 (BnDF5), which encodes a glycogen synthase kinase 3, BRASSINOSTEROID-INSENSITIVE 2 (BnaC03.BIN2), primarily controlling the elongation of basal internodes by inhibiting the elongation of internode cells. Genetic mapping and cloning revealed that BnDF5 is governed by a semi-dominant/dominant gene located on chromosome C03. Sequencing uncovered an SNP in BnaC03.BIN2 due to an amino acid substitution, which was confirmed via kompetitive allele-specific polymerase chain reaction marker analysis, and expressing the mutated BnaC03.BIN2 in the wild type resulted in decreased plant height. Practical breeding applications showed that heterozygous BnDF5 plants exhibited optimal intermediate height and strong yield heterosis, making the semi-dwarf mutant a valuable genetic resource for developing semi-dwarf rapeseed varieties with improved lodging resistance and yield.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"45"},"PeriodicalIF":3.9,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light regulates seed dormancy through FHY3-mediated activation of ACC OXIDASE 1 in Arabidopsis. 光通过fhy3介导的ACC氧化酶1激活调节拟南芥种子休眠。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-13 DOI: 10.1007/s11103-025-01559-9

Yitong Liu, Shuangrong Liu, Yanjun Jing, Jialong Li, Rongcheng Lin

{"title":"Light regulates seed dormancy through FHY3-mediated activation of ACC OXIDASE 1 in Arabidopsis.","authors":"Yitong Liu, Shuangrong Liu, Yanjun Jing, Jialong Li, Rongcheng Lin","doi":"10.1007/s11103-025-01559-9","DOIUrl":"10.1007/s11103-025-01559-9","url":null,"abstract":"Seed dormancy enables plants to delay germination until conditions are favorable for the survival of the next generation. Seed dormancy and germination are controlled by a combination of external and internal signals, in which light and ethylene act as critical regulators. However, how light and ethylene are interlinked to control these two processes remains to be investigated. Here, we show that ethylene and its precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), promote seed germination under light. Light facilitates the conversion of ACC to ethylene, in which phytochrome B (phyB) and FAR-RED ELONGATED HYPOCOTYL3 (FHY3) are functionally required. ACC oxidases (ACOs) catalyze the conversion of ACC to ethylene, among which ACO1 is specifically and predominantly expressed in imbibed seeds. Ethylene induces FHY3 protein accumulation in imbibed seeds, whereby FHY3 directly binds to ACO1 promoter and specifically mediates light-promoted ACO1 expression. Light promotes ACO1 protein accumulation. Overexpression of ACO1 significantly promotes seed germination, and almost completely restores the dormant defect of fhy3 loss-of-function mutants. In summary, this study reveals an ethylene-responsive regulatory cascade of phyB-FHY3-ACO1 that integrates external light input with internal factors to regulate seed dormancy and germination.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"44"},"PeriodicalIF":3.9,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0