The Arabidopsis F-box protein FBS associated with the helix-loop-helix transcription factor FAMA involved in stomatal immunity.

IF 3.9 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Molecular Biology Pub Date : 2025-03-20 DOI:10.1007/s11103-025-01577-7

Chunxia Zhang, Junling Yue, Shi Li, Chaoran Zuo, Yi Li, Qixiumei He, Jie Le

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引用次数: 0

Abstract

Stomatal pores serve as primary entry points for pathogen invasion. Stomatal closure is a crucial strategy that plants employ to counter pathogen attack. Here, we report that F-BOX STRESS-INDUCED (FBS) is essential for modulating stomatal closure, thereby enhancing resistance to bacteria in Arabidopsis thaliana. The fbs2-1 fbs3-1 fbs4-2 triple mutant displayed increased susceptibility to Pseudomonas syringae pv. tomato (PstDC3000) due to impaired stomatal closure. Additionally, FBS4 interacts with and degrades the basic helix-loop-helix (bHLH) transcription factor FAMA. Both the fama-1 single mutant plants and the fama-1 fbs2-1 fbs3-1 fbs4-2 quadruple mutant plants exhibited resistance to PstDC3000 inoculation. Furthermore, the expression levels of abscisic acid (ABA)-responsive genes RD29A, RD29B, ABI2, and CIPK25 were altered in the fbs2-1 fbs3-1 fbs4-2 and fama-1 mutant plants. Collectively, our data demonstrate that FBS, in association with FAMA, plays an important role in pathogen invasion by influencing ABA signaling-related stomatal closure.

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拟南芥F-box蛋白FBS与螺旋-环-螺旋转录因子FAMA相关，参与气孔免疫。

气孔是病原菌入侵的主要入口。气孔关闭是植物抵御病原菌侵袭的重要策略。在这里，我们报道了F-BOX应力诱导（FBS）对调节气孔关闭至关重要，从而增强了拟南芥对细菌的抗性。fbs2-1 - fbs1 -1 - fbs2- 2三重突变体对丁香假单胞菌pv的敏感性增加。番茄（PstDC3000）由于气孔关闭受损。此外，FBS4与基本螺旋-环-螺旋（bHLH）转录因子FAMA相互作用并降解。fama-1单突变株和fama-1 fbs2-1 fbs1 -1 fbs2- 2四突变株均表现出对PstDC3000接种的抗性。此外，脱落酸（ABA）应答基因RD29A、RD29B、ABI2和CIPK25在fbs2-1、fbs1 -1、fbs2- 2和fama-1突变株中的表达水平发生了改变。总的来说，我们的数据表明FBS与FAMA一起通过影响ABA信号相关的气孔关闭在病原体入侵中起重要作用。

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来源期刊

Plant Molecular Biology 生物-生化与分子生物学

自引率

2.00%

发文量

审稿时长

1.4 months

期刊介绍： Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.