Plant Molecular Biology最新文献_第9页

Identification of a drought stress response module in tomato plants commonly induced by fungal endophytes that confer increased drought tolerance. 鉴定番茄植物干旱胁迫响应模块，通常由真菌内生菌诱导，赋予增加耐旱性。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-12-17 DOI: 10.1007/s11103-024-01532-y

Adrián González Ortega-Villaizán, Eoghan King, Manish K Patel, Estefanía Rodríguez-Dobreva, Marcia González-Teuber, Patricio Ramos, Jesús Vicente-Carbajosa, Begoña Benito, Stephan Pollmann

{"title":"Identification of a drought stress response module in tomato plants commonly induced by fungal endophytes that confer increased drought tolerance.","authors":"Adrián González Ortega-Villaizán, Eoghan King, Manish K Patel, Estefanía Rodríguez-Dobreva, Marcia González-Teuber, Patricio Ramos, Jesús Vicente-Carbajosa, Begoña Benito, Stephan Pollmann","doi":"10.1007/s11103-024-01532-y","DOIUrl":"10.1007/s11103-024-01532-y","url":null,"abstract":"Global climate change exacerbates abiotic stresses, as drought, heat, and salt stresses are anticipated to increase significantly in the coming years. Plants coexist with a diverse range of microorganisms. Multiple inter-organismic relationships are known to confer benefits to plants, including growth promotion and enhanced tolerance to abiotic stresses. In this study, we investigated the mutualistic interactions between three fungal endophytes originally isolated from distinct arid environments and an agronomically relevant crop, Solanum lycopersicum. We demonstrated a significant increase in shoot biomass under drought conditions in co-cultivation with Penicillium chrysogenum isolated from Antarctica, Penicillium minioluteum isolated from the Atacama Desert, Chile, and Serendipita indica isolated from the Thar Desert, India. To elucidate plant gene modules commonly induced by the different endophytes that could explain the observed drought tolerance effect in tomato, a comprehensive transcriptomics analysis was conducted. This analysis led to the identification of a shared gene module in the fungus-infected tomato plants. Within this module, gene network analysis enabled us to identify genes related to abscisic acid (ABA) signaling, ABA transport, auxin signaling, ion homeostasis, proline biosynthesis, and jasmonic acid signaling, providing insights into the molecular basis of drought tolerance commonly mediated by fungal endophytes. Our findings highlight a conserved response in the mutualistic interactions between endophytic fungi isolated from unrelated environments and tomato roots, resulting in improved shoot biomass production under drought stress.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 1","pages":"7"},"PeriodicalIF":3.9,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11652604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary analysis of anthocyanin biosynthetic genes: insights into abiotic stress adaptation. 花青素生物合成基因的进化分析：对非生物胁迫适应的启示。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-12-16 DOI: 10.1007/s11103-024-01540-y

Sebastian Buitrago, Xinsun Yang, Lianjun Wang, Rui Pan, Wenying Zhang

{"title":"Evolutionary analysis of anthocyanin biosynthetic genes: insights into abiotic stress adaptation.","authors":"Sebastian Buitrago, Xinsun Yang, Lianjun Wang, Rui Pan, Wenying Zhang","doi":"10.1007/s11103-024-01540-y","DOIUrl":"10.1007/s11103-024-01540-y","url":null,"abstract":"Anthocyanin regulation can be fruitfully explored from a diverse perspective by studying distantly related model organisms. Land plants pioneers faced a huge evolutionary leap, involving substantial physiological and genetic changes. Anthocyanins have evolved alongside these changes, becoming versatile compounds capable of mitigating terrestrial challenges such as drought, salinity, extreme temperatures and high radiation. With the accessibility of whole-genome sequences from ancient plant lineages, deeper insights into the evolution of key metabolic pathways like phenylpropanoids have emerged. Despite understanding the function of anthocyanins under stress, gaps remain in uncovering the precise metabolic and regulatory mechanisms driving their overproduction under stressful conditions. For example, the regulatory effect of reactive oxygen species (ROS) on well-known transcription factors like MYBs is not fully elucidated. This manuscript presents an evolutionary analysis of the anthocyanin biosynthetic pathway to elucidate key genes. CINNAMATE 4-HYDROXYLASE (C4H) and CHALCONE ISOMERASE (CHI2) received particular attention. C4H exposes remarkable differences between aquatic and land plants, while CHI2 demonstrates substantial variation in gene copy number and sequence similarity across species. The role of transcription factors, such as MYB, and the involvement of ROS in the regulation of anthocyanin biosynthesis are discussed. Complementary gene expression analyses under abiotic stress in Arabidopsis thaliana, Selaginella moellendorffii, and Marchantia polymorpha reveal intriguing gene-stress relationships. This study highlights evolutionary trends and the regulatory complexity of anthocyanin production under abiotic stress, providing insights and opening avenues for future research.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 1","pages":"6"},"PeriodicalIF":3.9,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous expression of Halostachys caspica pathogenesis-related protein 10 increases salt and drought resistance in transgenic Arabidopsis thaliana. Halostachys caspica致病相关蛋白10的异源表达可提高转基因拟南芥的抗盐和抗旱能力。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-12-13 DOI: 10.1007/s11103-024-01536-8

Jing Cao, Ayixianmuguli Maitirouzi, Yudan Feng, Hua Zhang, Youqiang Heng, Jinbo Zhang, Yan Wang

{"title":"Heterologous expression of Halostachys caspica pathogenesis-related protein 10 increases salt and drought resistance in transgenic Arabidopsis thaliana.","authors":"Jing Cao, Ayixianmuguli Maitirouzi, Yudan Feng, Hua Zhang, Youqiang Heng, Jinbo Zhang, Yan Wang","doi":"10.1007/s11103-024-01536-8","DOIUrl":"10.1007/s11103-024-01536-8","url":null,"abstract":"Pathogenesis-related proteins (PR), whose expressions are induced by biotic and abiotic stress, play important roles in plant defense. Previous research identified the salt-induced HcPR10 gene in the halophyte Halostachys caspica as a regulator of plant growth and development through interactions with cytokinin. However, the mechanisms by which HcPR10 mediates resistance to abiotic stress remain poorly understood. In this study, we found that the heterologous expression of HcPR10 significantly enhanced salt and drought tolerance in Arabidopsis, likely by increasing the activity of antioxidant enzyme systems, allowing for effective scavenging of reactive oxygen species (ROS) and thus protecting plant cells from oxidative damage. Additionally, the overexpression of HcPR10 also activated the expression of stress-related genes in Arabidopsis. Furthermore, using yeast two-hybrid technology, five proteins (HcLTPG6, HcGPX6, HcUGT73B3, HcLHCB2.2, and HcMSA1) were identified as potential interacting partners for HcPR10, which could positively regulate the salt stress response mediated by HcPR10. Our findings lay the foundation for a better understanding of the molecular mechanisms of HcPR10 in response to abiotic stress and reveal additional candidate genes for improving crop salt tolerance through genetic engineering.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 1","pages":"5"},"PeriodicalIF":3.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SaTDT enhanced plant tolerance to NaCl stress by modulating the levels of malic acid and citric acid in cells. 通过调节细胞中苹果酸和柠檬酸的水平，SaTDT 增强了植物对 NaCl 胁迫的耐受性。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-12-12 DOI: 10.1007/s11103-024-01522-0

Xiangyu Wei, Li Xu, Suisui Dong, Nina He, Qianqian Xi, Dan Yao, Qianqian Wang, Yue Zuo, Chen Ling, Meiting Qi, Wen Bai, Kai Han, Yuwei Zhao, Long Tang, Yang Gao

{"title":"SaTDT enhanced plant tolerance to NaCl stress by modulating the levels of malic acid and citric acid in cells.","authors":"Xiangyu Wei, Li Xu, Suisui Dong, Nina He, Qianqian Xi, Dan Yao, Qianqian Wang, Yue Zuo, Chen Ling, Meiting Qi, Wen Bai, Kai Han, Yuwei Zhao, Long Tang, Yang Gao","doi":"10.1007/s11103-024-01522-0","DOIUrl":"10.1007/s11103-024-01522-0","url":null,"abstract":"The issue of soil salinization is a global concern that significantly impairs crop productivity, quality, and distribution. Tonoplast Dicarboxylate Transporter (TDT) is a pivotal malic acid transporter localized on the vacuolar membrane, involving in maintaining intracellular pH homeostasis in plants. However, the molecular mechanisms and regulatory pathways underlying plant salt tolerance through TDT remain elusive. In this study, we cloned a gene encoding vacuolar membrane dicarboxylic acid transporter designated as SaTDT from the halophyte Spartina alterniflora. Subsequently, its role in regulating salt stress was investigated. The heterologous expression of SaTDT in Arabidopsis thaliana was observed to enhance the transgenic plants' tolerance to salt stress and alleviate the growth damage caused by this stress. The overexpression of SaTDT can simultaneously enhance plant photosynthetic efficiency by regulating the cellular contents of malic acid and citric acid, or by increasing the activity of MDH and PEPC enzymes. It also regulates and balances energy utilization during carbon assimilation under salt-stressed conditions, thereby establishing an energetic foundation for enhancing plant tolerance to stress. SaTDT also has the capacity to enhance the plant cells' ability in regulating antioxidant enzyme activity or osmotic accumulation, thereby playing a crucial role in maintaining intracellular redox homeostasis. In conclusion, our findings establish a foundation basis for elucidating the regulatory role of the SaTDT gene in S.alterniflora's adaptation to high-salinity habitats.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 1","pages":"4"},"PeriodicalIF":3.9,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant growth Enhancement in Colchicine-Treated Tomato Seeds without Polyploidy Induction. 秋水仙素处理过的番茄种子在不诱导多倍体的情况下提高了植株生长。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-12-12 DOI: 10.1007/s11103-024-01521-1

Rosa Irma Obando-González, Luis Enrique Martínez-Hernández, Leandro Alberto Núñez-Muñoz, Berenice Calderón-Pérez, Roberto Ruiz-Medrano, José Abrahán Ramírez-Pool, Beatriz Xoconostle-Cázares

{"title":"Plant growth Enhancement in Colchicine-Treated Tomato Seeds without Polyploidy Induction.","authors":"Rosa Irma Obando-González, Luis Enrique Martínez-Hernández, Leandro Alberto Núñez-Muñoz, Berenice Calderón-Pérez, Roberto Ruiz-Medrano, José Abrahán Ramírez-Pool, Beatriz Xoconostle-Cázares","doi":"10.1007/s11103-024-01521-1","DOIUrl":"10.1007/s11103-024-01521-1","url":null,"abstract":"Plant breeding plays a pivotal role in the development of improved tomato cultivars, addressing various challenges faced by this crop worldwide. Tomato crop yield is affected by biotic and abiotic stress, including diverse pathogens and pests, extreme temperatures, drought, and soil salinity, thus affecting fruit quality, and overall crop productivity. Through strategic plant breeding approaches, it is possible to increase the genetic diversity of tomato cultivars, leading to the development of varieties with increased resistance to prevalent diseases and pests, improved tolerance to environmental stress, and enhanced adaptability to changing agroclimatic conditions. The induction of genetic variability using antimitotic agents, such as colchicine, has been widely employed in plant breeding precisely to this end. In this study, we analyzed the transcriptome of colchicine-treated tomato plants exhibiting larger size, characterized by larger leaves, while seedlings of the T2 generation harbored three cotyledons. A total of 382 differentially expressed genes encoding proteins associated with anatomical structure development, hormone synthesis and transport, flavonoid biosynthesis, and responses to various stimuli, stresses, and defense mechanisms were identified. Gene enrichment analysis suggests a role for auxin and flavonoid biosynthesis in cotyledon formation. Furthermore, single-nucleotide polymorphisms were mapped in colchicine-treated plants and determined which corresponded to differentially- expressed genes. Interestingly, most were associated to only a few genes in a similar location. This study provides significant insights into the genes and metabolic pathways affected in colchicine-treated tomatoes that exhibit improved agronomic traits, such as plant vigor and improved photosynthesis rate.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 1","pages":"3"},"PeriodicalIF":3.9,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11638462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GhCNGC31 is critical for conferring resistance to Verticillium wilt in cotton. GhCNGC31是棉花抗黄萎病的关键基因。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-12-12 DOI: 10.1007/s11103-024-01533-x

Tianming Li, Wenjing Jia, Lin Li, Shi Xu, Ruqiang Xu

{"title":"GhCNGC31 is critical for conferring resistance to Verticillium wilt in cotton.","authors":"Tianming Li, Wenjing Jia, Lin Li, Shi Xu, Ruqiang Xu","doi":"10.1007/s11103-024-01533-x","DOIUrl":"10.1007/s11103-024-01533-x","url":null,"abstract":"In the past decades, cyclic nucleotide-gated ion channels (CNGCs) have been extensively studied in diploid species Arabidopsis thaliana. However, the functional diversification of CNGCs in crop plants, mostly polyploid, remains poorly understood. In allotetraploid Upland cotton (Gossypium hirsutum), GhCNGC31 is one of the multiple orthologs of AtCNGC2, being present in the plasma membrane, capable of interacting with itself and binding to calmodulins and cyclic nucleotides. GhCNGC31 knockdown plants exhibited slight growth inhibition, and became more susceptible to Verticillium dahliae infection, which was associated with the reduced lignin and flavonoid accumulation, impaired ROS (reactive oxygen species) burst, and down-regulation of defense-related genes PR1, JAZ2, LOX2, and RBOH10. RNA-Seq analysis identified 1817 differentially expressed genes from GhCNGC31 knockdown, of which 1184 (65%) were responsive to V. dahliae infection and accounted for 57% among a total of 2065 V. dahliae-responsive genes identified in this study. These GhCNGC31-regulated genes mainly function with cell wall organization and biogenesis, cellular carbohydrate metabolic or biosynthetic process, cellular component macromolecule biosynthetic process, and rhythmic process. They are significantly enriched in the pathways of plant MAPK signaling, plant-pathogen interaction, phenylpropanoid biosynthesis, and plant hormone signal transduction. A set of transcription factors (TFs) and resistance (R) genes are among the GhCNGC31-regulated genes, which are significantly over-represented with the TCP and WRKY TFs families, as well as with the R genes of T (TIR) and TNL (TIR-NB-LRR) classes. Together, our results unraveled a critical role of GhCNGC31 for conferring resistance to Verticillium wilt in cotton.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 1","pages":"2"},"PeriodicalIF":3.9,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 2-oxoglutarate-dependent dioxygenase, GLUCORAPHASATIN SYNTHASE 1 (GRS1) is a major determinant for different aliphatic glucosinolates between radish and Chinese cabbage. 2-氧戊二酸依赖的双加氧酶，GLUCORAPHASATIN SYNTHASE 1 （GRS1）是萝卜和白菜之间不同脂肪型硫代葡萄糖苷的主要决定因素。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-12-10 DOI: 10.1007/s11103-024-01537-7

Peter Choi, Adji Baskoro Dwi Nugroho, Heewon Moon, Dong-Hwan Kim

{"title":"A 2-oxoglutarate-dependent dioxygenase, GLUCORAPHASATIN SYNTHASE 1 (GRS1) is a major determinant for different aliphatic glucosinolates between radish and Chinese cabbage.","authors":"Peter Choi, Adji Baskoro Dwi Nugroho, Heewon Moon, Dong-Hwan Kim","doi":"10.1007/s11103-024-01537-7","DOIUrl":"10.1007/s11103-024-01537-7","url":null,"abstract":"Glucosinolates (GSLs) are secondary metabolites in Brassicaceae plants and play a defensive role against a variety of abiotic and biotic stresses. Also, it exhibits anti-cancer activity against cancer cell in human. Different profiles of aliphatic GSL compounds between radish and Chinese cabbage were previously reported. However, molecular details underlying the divergent profile between two species were not clearly understood. In this study, we found that major difference of aliphatic GSLs profiles between two species is determined by the dominantly expressed genes in first step of the secondary modification phase, which are responsible for enzymatic catalysis of methylthioalkyl-glucosinolate. For instance, active expression of GLUCORAPHASATIN SYNTHASE 1 (GRS1) gene in radish play an important role in the production of glucoraphasatin (GRH) and glucoraphenin (GRE), a major aliphatic GSLs in radish. Meanwhile, Chinese cabbage was found to merely produce glucoraphasatin (GRH), instead producing glucoraphanin (GRA) and gluconapin (GNP) due to the mere expression of GRS1 homologs and abundant expressions of FLAVIN-CONTAINING MONOOXYGENASES (FMO GS-OX) homologs in Chinese cabbage. In addition, we noticed that wounding treatment on leaf tissues substantially enhanced the production of aliphatic and benzenic GSLs in both Chinese cabbage and radish, indicating that GSLs are wound-induced defensive compounds in both Chinese cabbage and radish plants.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 1","pages":"1"},"PeriodicalIF":3.9,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogen peroxide priming triggers splicing memory in grape berries. 过氧化氢引物触发葡萄浆果中的拼接记忆。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-11-28 DOI: 10.1007/s11103-024-01528-8

Ding-Ding Zuo, Hao-Ting Sun, Lu Yang, Meng-Ling Zheng, Jing Zhang, Da-Long Guo

{"title":"Hydrogen peroxide priming triggers splicing memory in grape berries.","authors":"Ding-Ding Zuo, Hao-Ting Sun, Lu Yang, Meng-Ling Zheng, Jing Zhang, Da-Long Guo","doi":"10.1007/s11103-024-01528-8","DOIUrl":"10.1007/s11103-024-01528-8","url":null,"abstract":"Plants are highly sensitive to environmental changes, and alternative splicing (AS) has been described in many studies due to its important control role in stress response. Recent studies indicated that plants exhibit splicing memory to stress to effectively activate transcriptional adaptation. Hydrogen peroxide (H2O2), as a reactive oxygen species (ROS), has toxic effects on plants. However, it also has a significant effect on promoting early maturity of 'Kyoho' grape at low concentrations. To explore the mechanism of priming treatment of H2O2 showing better promotion effect, the RNA-Seq data of H2O2-primied and no-primied fruits were analyzed. The genes with H2O2 stress splicing memory were identified, accompanied by changes in H3K4me3 modification levels, and their splicing memory patterns were verified by PCR and agarose gel electrophoresis. This finding establishes a link between alternative splicing memory and fruit ripening under H2O2 regulation and contribute to develop the application of H2O2 in fruit ripening.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"114 6","pages":"129"},"PeriodicalIF":3.9,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142740177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolite and transcriptome reveal the lipid-associated key components and genes regulated by BoORP3a in ornamental kale. 代谢物和转录组揭示了观赏甘蓝中与脂质相关的关键成分和受 BoORP3a 调控的基因。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-11-28 DOI: 10.1007/s11103-024-01524-y

Zheng Liu, Fuhui Zhou, Yashu Li, Xin Feng, Pengfang Zhu

{"title":"Metabolite and transcriptome reveal the lipid-associated key components and genes regulated by BoORP3a in ornamental kale.","authors":"Zheng Liu, Fuhui Zhou, Yashu Li, Xin Feng, Pengfang Zhu","doi":"10.1007/s11103-024-01524-y","DOIUrl":"10.1007/s11103-024-01524-y","url":null,"abstract":"BoORP3a, an oxysterol-binding protein, located in the endoplasmic reticulum (ER), may function in cuticular wax deposition in ornamental kale. In this study, we investigated its regulation of the key components of cuticular wax and lipids, metabolic pathways, and potential target genes. HS-SPME/GC-MS identified 34 and 31 volatile organic compounds in wild-type and the BoORP3a-overexpressing plant OE-ORP3a-7, respectively, primarily including alkane, ketone, ester, and alcohol. Hentriacontane, 15-nonacosanone, and > C20 alkanes were more abundant in OE-ORP3a-7, which may result in more cuticular wax in this plant. RNA sequencing identified 223 differentially expressed genes (DEGs) between wild-type and OE-ORP3a-7, comprising 119 upregulated and 104 downregulated DEGs. The KEGG enrichment analysis revealed that the downregulated DEGs in OE-ORP3a-7 were involved in glyoxylate and dicarboxylate metabolism, SNARE (Soluble N-ethylmaleimide-sensitive factor attachment protein receptor) interactions in vesicular transport, fatty acid biosynthesis, and glycerolipid metabolism; the upregulated DEGs were involved in steroid biosynthesis, fatty acid degradation, alpha-linolenic acid metabolism, and sphingolipid metabolism. Bo1g106990, Bo1g123670, and Bo9g166090 were identified as key DEGs in lipid-related pathways. We speculate that BoORP3a regulates several lipid metabolisms and may coordinate lipid turnover and remodeling. The results of this study will enrich the functionality of the ORPs family, provide new insights into plant wax research, and have significant implications for ornamental kale breeding.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"114 6","pages":"130"},"PeriodicalIF":3.9,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142740179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms of plant productivity enhancement by nano fertilizers for sustainable agriculture. 纳米肥料提高植物生产力的分子机制，促进可持续农业。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-11-26 DOI: 10.1007/s11103-024-01527-9

Arpan Dey, Ayan Sadhukhan

{"title":"Molecular mechanisms of plant productivity enhancement by nano fertilizers for sustainable agriculture.","authors":"Arpan Dey, Ayan Sadhukhan","doi":"10.1007/s11103-024-01527-9","DOIUrl":"10.1007/s11103-024-01527-9","url":null,"abstract":"Essential plant nutrients encapsulated or combined with nano-dimensional adsorbents define nano fertilizers (NFs). Nanoformulation of non-essential elements enhancing plant growth and stress tolerance also comes under the umbrella of NFs. NFs have an edge over conventional chemical fertilizers, viz., higher plant biomass and yield using much lesser fertilization, thereby reducing environmental pollution. Foliar and root applications of NFs lead to their successful uptake by the plant, depending on the size, surface charge, and other physicochemical properties of NFs. Smaller NFs can pass through channels on the waxy cuticle depending on the hydrophobicity, while larger NFs pass through the stomatal conduits of leaves. Charge-based adsorption, followed by apoplastic movement and endocytosis, translocates NFs through the root, while the size of NFs influences passage into vascular tissues. Recent transcriptomic, proteomic, and metabolomic studies throw light on the molecular mechanisms of growth promotion by NFs. The expression levels of nutrient transporter genes are regulated by NFs, controlling uptake and minimizing excess nutrient toxicity. Accelerated growth by NFs is brought about by their extensive regulation of cell division, photosynthesis, carbohydrate, and nitrogen metabolism, as well as the phytohormone-dependent signaling pathways related to development, stress response, and plant defense. NFs mimic Ca,2+ eliciting second messengers and associated proteins in signaling cascades, reaching transcription factors and finally orchestrating gene expression to enhance growth and stress tolerance. Developing advanced nano fertilizers of the future must involve exploring molecular interactions with plants to reduce toxicity and improve effectiveness.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"114 6","pages":"128"},"PeriodicalIF":3.9,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0