Plant Molecular Biology最新文献_第9页

Genome-wide identification and functional characterization of the CaD27 gene family in pepper under cold stress. 低温胁迫下辣椒CaD27基因家族的全基因组鉴定及功能表征

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-02-20 DOI: 10.1007/s11103-026-01688-9

Jian Li, Jie Li, Hongbo Fu, Yanzhuang Wang, Juan Li, Minkun Pei, Jihua Yu, Ping Yang

{"title":"Genome-wide identification and functional characterization of the CaD27 gene family in pepper under cold stress.","authors":"Jian Li, Jie Li, Hongbo Fu, Yanzhuang Wang, Juan Li, Minkun Pei, Jihua Yu, Ping Yang","doi":"10.1007/s11103-026-01688-9","DOIUrl":"10.1007/s11103-026-01688-9","url":null,"abstract":"Strigolactones (SLs) are crucial phytohormones involved in plant growth and stress adaptation. However, the role of SL biosynthetic genes, particularly CaD27, in pepper under cold stress remains unclear. In this study, we identified three CaD27 genes in the pepper genome, all harboring promoter regions enriched with phytohormone and stress-responsive cis-elements. Functional analysis via virus-induced gene silencing (VIGS) revealed that silencing CaD27.1 or CaD27.3 markedly impaired cold tolerance. Under cold stress, silenced plants exhibited excessive accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), along with elevated relative electrolyte leakage (REL). Concurrently, antioxidant enzyme activities and the expression of related genes were significantly suppressed. The expression of key cold-responsive genes was also downregulated. Furthermore, abscisic acid (ABA) biosynthesis was compromised, as evidenced by reduced levels of ABA and downregulated expression of ABA synthesis genes (CaNCED1, CaABA2, CaAAO4) and the receptor gene CaPYL2, whereas the ABA catabolism gene CaCYP707A4 remained unchanged. This ABA deficiency led to impaired stomatal closure and exacerbated wilting. Our findings demonstrate that CaD27.1 and CaD27.3 positively regulate cold tolerance in pepper by modulating ROS scavenging and ABA-mediated stomatal responses. Collectively, our findings identify CaD27.1 and CaD27.3 as positive regulators of cold tolerance in pepper and establish them as promising candidate genes for breeding cold-resilient pepper varieties.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146258544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introgression of barley chromosome arms 4H and 6H into wheat via Robertsonian translocations: GBS-assisted structural analysis and impact on grain nutrient composition. 大麦染色体臂4H和6H通过罗伯逊易位渗入小麦：gbs辅助结构分析及其对籽粒营养成分的影响

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-02-18 DOI: 10.1007/s11103-026-01689-8

László Ivanizs, Eszter Gaál, Klaudia Kruppa, András Farkas, Péter Mikó, Edina Türkösi, Marianna Rakszegi, Péter Kovács, Balázs Kalapos, Andrea Gulyás, Norbert Hidvégi, Kitti Szőke-Pázsi, Márta Molnár-Láng, Éva Szakács, Mahmoud Said, Jan Bartoš, Tünde Pusztahelyi, Dimitar Douchkov, István Molnár

{"title":"Introgression of barley chromosome arms 4H and 6H into wheat via Robertsonian translocations: GBS-assisted structural analysis and impact on grain nutrient composition.","authors":"László Ivanizs, Eszter Gaál, Klaudia Kruppa, András Farkas, Péter Mikó, Edina Türkösi, Marianna Rakszegi, Péter Kovács, Balázs Kalapos, Andrea Gulyás, Norbert Hidvégi, Kitti Szőke-Pázsi, Márta Molnár-Láng, Éva Szakács, Mahmoud Said, Jan Bartoš, Tünde Pusztahelyi, Dimitar Douchkov, István Molnár","doi":"10.1007/s11103-026-01689-8","DOIUrl":"10.1007/s11103-026-01689-8","url":null,"abstract":"In addition to wild relatives, cultivated Triticeae species such as barley can also serve as an excellent gene source to improve the agronomic traits of bread wheat. The Asakaze-Manas addition lines previously developed in Martonvásár exhibit low fertility and unstable inheritance; nonetheless, they could serve as ’bridge materials’ for the incorporation of barley chromatin into the wheat genome. By leveraging the centric breakage-fusion mechanism of unpaired chromosomes and the gametocidal effect of chromosome 2C from Aegilops cylindrica, the 4H and 6H addition lines were used to develop genetically stable translocation genotypes. In situ hybridization and molecular marker analyses were applied to identify the wheat and barley chromosome arms in the compensatory translocations, designated as T6HS.6BL, T6BS.6HL and T4BS.4HL. These results were confirmed by GBS read coverage analysis, which revealed a putative pericentric inversion in the barley chromosome arms involved in the T6HS.6BL and T6BS.6HL. The comparable agronomic performance of the centric fusion lines indicates that these chromosomal rearrangements had minimal, if any, negative impact relative to the parental wheat varieties. The newly developed translocation lines had an increased amount of minerals such as iron, suggesting that they could serve as promising genetic material for the biofortification of hexaploid wheat.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":"23"},"PeriodicalIF":3.7,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12916549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146220705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic analysis of fatty acid and cannabinoid synthases in an S1 cannabis progeny segregating for propyl cannabinoid biosynthesis. 大麻S1后代分离丙基大麻素生物合成的脂肪酸和大麻素合成酶的转录组学分析。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-02-17 DOI: 10.1007/s11103-026-01690-1

Samuel Haiden, Anthony Torres, Anthony Provatas, Gerald Berkowitz

{"title":"Transcriptomic analysis of fatty acid and cannabinoid synthases in an S1 cannabis progeny segregating for propyl cannabinoid biosynthesis.","authors":"Samuel Haiden, Anthony Torres, Anthony Provatas, Gerald Berkowitz","doi":"10.1007/s11103-026-01690-1","DOIUrl":"10.1007/s11103-026-01690-1","url":null,"abstract":"The biosynthesis of cannabinoids possessing a 5-carbon acyl-tail (cannabigerolic acid (CBGa), tetrahydrocannabinolic acid (THCa), and cannabidiolic acid (CBDa)) in C. sativa is relatively well characterized, but several other families of cannabinoids with various acyl-tail lengths exist. This study aims to deepen our understanding of three carbon (C3, propyl) cannabinoid biosynthesis. Existing theories suggest that two alleles, a truncated BETA-KETOACYL-REDUCTASE (BKR) mutant and an ACYL-LIPID-THIOESTERASE paralog (ALT4), are responsible for conferring propyl-cannabinoid biosynthesis. This study evaluated several factors across an S1 progeny segregating for varin accumulation. We demonstrate that C3 and C5 dominant chemovars exhbit different patterns in total acid cannabinoid (TAC) accumulation, and that the ratio of C3:C5 cannabinoids in high C3 individuals follows a distinct pattern which correlates with ALT4 expression. In the high-C3 group, we discovered that the butanoic acid ratio was significantly higher than the high-C5 group. We also show that the ratio of C3:C5 cannabinoids is much higher in vegetative leaves than mature flowers. Several genes that are critical to cannabinoid biosynthesis were differentially expressed across 6 weeks of flowering between the C3 and C5 groups, suggesting potential mechanisms for chemotype differentiation. We characterized the expression pattern of ALT4 and demonstrate that neither the overexpression of ALT4 nor the overexpression of a truncated, 7-exon BKR mutant yield an increased propyl cannabinoid ratio. Finally, we demonstrate that nearly all of the C3 individuals had a unique SNP pattern in the BKR locus, while almost none of the C5 individuals had any variation at the BKR locus.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":"22"},"PeriodicalIF":3.7,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146207267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allele-specific chromatin dynamics underpin heterosis and shoot development in hybrid poplar 'Nanlin895'. 杂交杨‘南林895’杂种优势和芽部发育的等位基因特异性染色质动力学基础

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-02-11 DOI: 10.1007/s11103-026-01684-z

Ying-Chang Mei, Yong-Xia Chen, Yu Guo, Yu-Ying Liu, Sheng-Ying Zhang, Xiao-Xia Zhang, Bo Xu, Bai-Chen Wang, Qing Chao

{"title":"Allele-specific chromatin dynamics underpin heterosis and shoot development in hybrid poplar 'Nanlin895'.","authors":"Ying-Chang Mei, Yong-Xia Chen, Yu Guo, Yu-Ying Liu, Sheng-Ying Zhang, Xiao-Xia Zhang, Bo Xu, Bai-Chen Wang, Qing Chao","doi":"10.1007/s11103-026-01684-z","DOIUrl":"10.1007/s11103-026-01684-z","url":null,"abstract":"In our prior research on the fast-growing hybrid poplar Populus deltoides × Populus euramericana cv. ‘Nanlin895’, we identified key growth-related genes using the Populus trichocarpa genome as a reference. However, many discovered elements—such as newly annotated transcripts, promoters, regulatory sequences, and open chromatin regions—are poorly characterized, a situation often hindered by an incomplete genome assembly. These elements may critically influence shoot development through the regulation of quantitative traits. In this study, we validate the hybrid nature of ‘Nanlin895,’ showing that subgenome A (subA) and subB reflect the characteristics of P. deltoides and P. trichocarpa, respectively. We also illustrate the complementary expansions and contractions of gene families that contributed to allelic diversity. Subsequently, we conducted a detailed analysis of allele-specific gene expression, chromatin structure, and open chromatin regions in the shoot apical meristem, internodes 1–3, and internodes 4–5, which represent a gradient of shoot development. Analysis of asymmetrical expression identified 6279 genes, 3154 from subA and 3125 subB genes, that were differentially expressed during shoot development and revealed that subA and subB exhibit distinct expression patterns; for instance, genes associated with vascular functions are mainly expressed in subB, while those related to cell division are predominantly expressed in subA. By profiling topologically associating domains and open chromatin regions, we linked chromatin features to the regulation of key genes (e.g., CESAs, HB51, WOXs) that were expressed in different regions of the stem. These results reveal the genetic and three-dimensional chromatin basis of heterosis in ‘Nanlin895’, paving the way for molecular breeding strategies to enhance forestry and bioenergy traits.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":"21"},"PeriodicalIF":3.7,"publicationDate":"2026-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146157958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated analysis of transcriptome and proteome under salinity stress from leaf tissues of Pisum sativum. 盐胁迫下Pisum sativum叶片组织转录组和蛋白质组的综合分析。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-02-09 DOI: 10.1007/s11103-026-01685-y

Kunal Dhokne, Pavithra Ramachandran, Jayendra Pandey, Naveen Kumar Pandey, Rajagopal Subramanyam

{"title":"Integrated analysis of transcriptome and proteome under salinity stress from leaf tissues of Pisum sativum.","authors":"Kunal Dhokne, Pavithra Ramachandran, Jayendra Pandey, Naveen Kumar Pandey, Rajagopal Subramanyam","doi":"10.1007/s11103-026-01685-y","DOIUrl":"10.1007/s11103-026-01685-y","url":null,"abstract":"High salinity is a prevalent and critical abiotic stress that significantly hampers plant growth and disrupts the delicate balance between photosynthetic electron transport and the utilization of ATP and NADPH. To elucidate the genetic regulation underlying salt stress responses, we performed a comprehensive analysis integrating transcriptomic and proteomic profiling in leaf tissue of Pisum sativum (pea) exposed to 200 mM NaCl. Following stringent screening of the mRNA-seq and proteomic data, approximately 89 genes/proteins were identified as coordinately differentially expressed at both the proteomic and mRNA levels. Key components of photosystem II (psbA, psbP), cyclic electron transport (pgrl1A, pnsB1), ion homeostasis (kea3), and carotenoid biosynthesis (lcy1, zep) were transcriptionally and translationally suppressed. These results were further validated by qRT-PCR, which mimiced the transcriptome and proteome data. Gene Ontology and KEGG enrichment analysis highlighted a systemic shift from energy acquisition towards stress defense and cellular protection. Together, our multi-omics investigation provides a mechanistic framework for understanding salinity tolerance in P. sativum, offering molecular targets for the development of stress-resilient legumes.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":"20"},"PeriodicalIF":3.7,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146143079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous hydrogen sulfide enhances the resistance of cabbage seedlings to black rot by improving phenolic synthesis and reducing reactive oxygen species. 外源硫化氢通过促进酚类合成和减少活性氧来增强白菜幼苗对黑腐病的抗性。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-02-06 DOI: 10.1007/s11103-026-01683-0

Xinkai Liu, Huiping Wang, Zhibin Yue, Jie Wang, Jue Wang, Tingting Dou, Lina Zheng, Xinyue Liu, Haojie Dai, Jihua Yu, Zeci Liu

{"title":"Exogenous hydrogen sulfide enhances the resistance of cabbage seedlings to black rot by improving phenolic synthesis and reducing reactive oxygen species.","authors":"Xinkai Liu, Huiping Wang, Zhibin Yue, Jie Wang, Jue Wang, Tingting Dou, Lina Zheng, Xinyue Liu, Haojie Dai, Jihua Yu, Zeci Liu","doi":"10.1007/s11103-026-01683-0","DOIUrl":"10.1007/s11103-026-01683-0","url":null,"abstract":"Black rot, caused by Xanthomonas campestris pv. campestris (Xcc), is a devastating disease in cabbage. As a gaseous signaling molecule, hydrogen sulfide (H2S) is widely involved in plant disease resistance. However, the mechanism by which H2S enhances resistance to Xcc remains unclear. In our study, we demonstrate that exogenous H2S significantly enhances cabbage resistance to black rot by enhancing the phenylpropanoid pathway and antioxidant system. Physiological and biochemical analyses revealed that H2S pretreatment boosted endogenous H2S and nitric oxide (NO) levels by over 18.8% and 107.7%, respectively, and increased the contents of lignin and tannin by more than 16%. Concurrently, H2S reduced hydrogen peroxide (H2O2) accumulation by 10.9% and the superoxide anion (O2−) production rate by 42.2%, while enhancing the scavenging rates of superoxide and hydroxyl radicals. Physiological and biochemical results indicated that H2S application markedly promoted the accumulation of key phenolic acids, including protocatechuic acid (108.2%), chlorogenic acid (40.8%), and ferulic acid (68.9%), by upregulating the activities and related gene (PAL, C4H, COMT) expression level of pivotal enzymes. Integrated transcriptomic and metabolomic analyses further confirmed that H2S activated the phenylpropanoid biosynthesis pathway. Our findings providing new insights into the molecular mechanism of H2S-mediated disease resistance and a potential strategy for controlling black rot.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":"19"},"PeriodicalIF":3.7,"publicationDate":"2026-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146132057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering the molecular basis of high morphinan product efficiency in opium poppy through Multi-omics integrated analysis with multi-capsules. 通过多胶囊的多组学综合分析，揭示罂粟中吗啡肽高产物效率的分子基础。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-02-05 DOI: 10.1007/s11103-026-01682-1

Yumin Huang, Mao Sun, Wuhu Gong, Yifeng Liu, Yimeng Cheng, Lijuan Yuan, Yuanming Wu

{"title":"Uncovering the molecular basis of high morphinan product efficiency in opium poppy through Multi-omics integrated analysis with multi-capsules.","authors":"Yumin Huang, Mao Sun, Wuhu Gong, Yifeng Liu, Yimeng Cheng, Lijuan Yuan, Yuanming Wu","doi":"10.1007/s11103-026-01682-1","DOIUrl":"10.1007/s11103-026-01682-1","url":null,"abstract":"Exploring the genetic landscape and corresponding regulatory mechanisms influencing morphine and related alkaloid production efficiency in the opium poppy (Papaver Somniferum L.) is important for sustainable medicinal production. However, information regarding the corresponding medicinal properties of poppy subspecies remains elusive, and the correlation between agronomic traits and morphine production remains unclear. this study aims to investigate the distinction between two poppy subspecies: Shan Yang (SY) and Lan Tian (LT). Physiological data revealed that SY exhibited greater tolerance to environmental stress and a higher morphine content than LT, especially during the reproductive stage. To investigate genetic and molecular mechanisms underlying different tolerance traits and morphine synthesis efficiencies, a comprehensive comparison of genomic, transcriptomic, and metabolomic data was conducted.The results showing significantly higher single nucleotide polymorphism (SNP) frequency in SY (23.78%) compared to LT (23.69%). The proportions of SKIP (Skipped Exon) and MIR (Multi Intron Retention) were higher in SY than in LT. SY showed significantly higher gene expression related to benzylisoquinoline alkaloid synthesis, especially those involved in the steps from 1,2 dehydroreticulinium to morphine, narcotoline and noscapine, compared to LT. The upregulated expression of phenylpropanoid biosynthesis-related genes contributes to its more resistant agronomical traits. It plays a synergistic role in alkaloid production. The higher morphine content in SY resulted from an integrated effect controlled at different levels. Our findings offer new insights into the molecular mechanisms of morphine synthesis and present valuable gene resources for improving poppy cultivars with higher morphine content. ","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":"18"},"PeriodicalIF":3.7,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146125963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The non-catalytic binding of auxin, cytokinin and salicylic acid by tomato Phi class glutathione transferases: insights from computational modelling. 番茄Phi类谷胱甘肽转移酶对生长素、细胞分裂素和水杨酸的非催化结合：来自计算模型的见解。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-02-03 DOI: 10.1007/s11103-026-01681-2

Ádám Barnabás Hajnal, Ágnes Gallé, Jolán Csiszár

{"title":"The non-catalytic binding of auxin, cytokinin and salicylic acid by tomato Phi class glutathione transferases: insights from computational modelling.","authors":"Ádám Barnabás Hajnal, Ágnes Gallé, Jolán Csiszár","doi":"10.1007/s11103-026-01681-2","DOIUrl":"10.1007/s11103-026-01681-2","url":null,"abstract":"Glutathione transferases (GSTs, EC 2.5.1.18) are a diverse enzyme family involved in various cellular processes, regulated by several stimuli, including plant hormones (phytohormones). Additionally, GSTs can also influence hormone levels, as certain GSTs, particularly members of the Phi class GSTs (GSTFs) have been reported to directly bind small phytohormones (auxin, cytokinin, and salicylic acid). However, the structural aspects of these interactions remained unresolved. It has been documented that an Arabidopsis thaliana GSTF, AtGSTF2, possesses unique non-catalytic ligand-binding sites (L sites). This study focuses on the orthologous tomato (Solanum lycopersicum) GSTFs (SlGSTFs) and explores the connection between this non-catalytic ligand-binding function and phytohormones. Using modern in silico techniques, such as protein modelling by AlphaFold, molecular docking, and molecular dynamics (MD) simulations, we provide the first insights into the possible phytohormone-binding ability of SlGSTFs. In particular, SlGSTF5 shows significant potential for a novel phytohormone binding function, thereby broadening the potential roles of plant GSTs. Our work also includes a simple protocol for modelling and analysing protein-ligand interactions, offering valuable insights into the role of individual plant GSTs.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":"17"},"PeriodicalIF":3.7,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12868010/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146113910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applications of omics in deciphering the unique traits of durian, the king of fruits. 应用组学技术解读水果之王榴莲的独特性状。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-02-03 DOI: 10.1007/s11103-025-01677-4

Chee-Wei Yew, Ching-Ching Wee

引用次数: 0

Unravelling the molecular mechanisms of vegetative-to-reproductive transition in Cynara cardunculus by RNA-Seq analysis. 利用RNA-Seq分析揭示红桃属植物向生殖过渡的分子机制。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-01-31 DOI: 10.1007/s11103-025-01679-2

A Paulino, I Fernandes, R C Pires, A Usié, A Faustino, J Santos, T Brás, D Rosa, O S Paulo, M F Duarte, L Marum

{"title":"Unravelling the molecular mechanisms of vegetative-to-reproductive transition in Cynara cardunculus by RNA-Seq analysis.","authors":"A Paulino, I Fernandes, R C Pires, A Usié, A Faustino, J Santos, T Brás, D Rosa, O S Paulo, M F Duarte, L Marum","doi":"10.1007/s11103-025-01679-2","DOIUrl":"10.1007/s11103-025-01679-2","url":null,"abstract":"Cynara cardunculus inflorescence plays a significant role in cheese manufacturing and human consumption due to the milk-clotting capacity and the production of edible immature capitula. Consequently, it is crucial to understand the complex cellular and molecular processes involved in the development of cardoon flowers. The current study used western wild cardoon in two developmental stages, vegetative and reproductive, to understand the molecular mechanisms mediating cardoon`s vegetative-to-reproductive transition. Transcriptome profiling identified 552 differentially expressed genes (DEGs) between vegetative and reproductive stages, with 321 upregulated in stage 4 (vegetative state) and 231 in stages 5/6 (reproductive state). Gene Ontology (GO) analysis revealed DEGs involved in biological processes such as oxidation-reduction, lipid metabolism, and defense responses. Metabolic pathways, including phenylpropanoid biosynthesis and anther/pollen development, were significantly enriched. Genes associated with lignin formation, flavonoid synthesis, and acetyltransferase activity were upregulated in the vegetative stage. At the same time, those related to flower development and sesquiterpene lactone biosynthesis were prominent in the reproductive stage. Transcription factors, including MADS-box and ethylene-responsive families, were critical in regulating developmental transitions. This study provides comprehensive insights into the morphological, chemical, and molecular dynamics of C. cardunculus across its phenological stages, underscoring the plant’s adaptability and potential for pharmaceutical and industrial applications. The findings highlight the importance of seasonal and developmental timing in optimizing the production of valuable secondary metabolites, such as cynaropicrin.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 1","pages":"15"},"PeriodicalIF":3.7,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12860834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146093830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0