Plant Molecular Biology最新文献_第7页

Straightforward and affordable agroinfiltration with RUBY accelerates RNA silencing research. 使用 RUBY 进行直接、经济的农业过滤可加速 RNA 沉默研究。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-05-19 DOI: 10.1007/s11103-024-01463-8

Midori Tabara, Ayumi Matsumoto, Yuriko Kibayashi, Atsushi Takeda, Kazuki Motomura

{"title":"Straightforward and affordable agroinfiltration with RUBY accelerates RNA silencing research.","authors":"Midori Tabara, Ayumi Matsumoto, Yuriko Kibayashi, Atsushi Takeda, Kazuki Motomura","doi":"10.1007/s11103-024-01463-8","DOIUrl":"10.1007/s11103-024-01463-8","url":null,"abstract":"Transient expression and induction of RNA silencing by agroinfiltration is a fundamental method in plant RNA biology. Here, we introduce a new reporter assay using RUBY, which encodes three key enzymes of the betalain biosynthesis pathway, as a polycistronic mRNA. The red pigmentation conferred by betalains allows visual confirmation of gene expression or silencing levels without tissue disruption, and the silencing levels can be quantitatively measured by absorbance in as little as a few minutes. Infiltration of RUBY in combination with p19, a well-known RNA silencing suppressor, induced a fivefold higher accumulation of betalains at 7 days post infiltration compared to infiltration of RUBY alone. We demonstrated that co-infiltration of RUBY with two RNA silencing inducers, targeting either CYP76AD1 or glycosyltransferase within the RUBY construct, effectively reduces RUBY mRNA and betalain levels, indicating successful RNA silencing. Therefore, compared to conventional reporter assays for RNA silencing, the RUBY-based assay provides a simple and rapid method for quantitative analysis without the need for specialized equipment, making it useful for a wide range of RNA silencing studies.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyruvate kinase 2 from Synechocystis sp. PCC 6803 increased substrate affinity via glucose-6-phosphate and ribose-5-phosphate for phosphoenolpyruvate consumption. 来自 Synechocystis sp. PCC 6803 的丙酮酸激酶 2 通过葡萄糖-6-磷酸和核糖-5-磷酸提高了消耗磷酸烯醇丙酮酸的底物亲和力。

IF 5.1 2区生物学

Plant Molecular Biology Pub Date : 2024-05-17 DOI: 10.1007/s11103-023-01401-0

Masahiro Karikomi, N. Katayama, T. Osanai

引用次数: 0

Pyruvate kinase 2 from Synechocystis sp. PCC 6803 increased substrate affinity via glucose-6-phosphate and ribose-5-phosphate for phosphoenolpyruvate consumption. 来自 Synechocystis sp. PCC 6803 的丙酮酸激酶 2 通过葡萄糖-6-磷酸和核糖-5-磷酸提高了消耗磷酸烯醇丙酮酸的底物亲和力。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-05-17 DOI: 10.1007/s11103-023-01401-0

Masahiro Karikomi, Noriaki Katayama, Takashi Osanai

{"title":"Pyruvate kinase 2 from Synechocystis sp. PCC 6803 increased substrate affinity via glucose-6-phosphate and ribose-5-phosphate for phosphoenolpyruvate consumption.","authors":"Masahiro Karikomi, Noriaki Katayama, Takashi Osanai","doi":"10.1007/s11103-023-01401-0","DOIUrl":"10.1007/s11103-023-01401-0","url":null,"abstract":"Pyruvate kinase (Pyk, EC 2.7.1.40) is a glycolytic enzyme that generates pyruvate and adenosine triphosphate (ATP) from phosphoenolpyruvate (PEP) and adenosine diphosphate (ADP), respectively. Pyk couples pyruvate and tricarboxylic acid metabolisms. Synechocystis sp. PCC 6803 possesses two pyk genes (encoded pyk1, sll0587 and pyk2, sll1275). A previous study suggested that pyk2 and not pyk1 is essential for cell viability; however, its biochemical analysis is yet to be performed. Herein, we biochemically analyzed Synechocystis Pyk2 (hereafter, SyPyk2). The optimum pH and temperature of SyPyk2 were 7.0 and 55 °C, respectively, and the Km values for PEP and ADP under optimal conditions were 1.5 and 0.053 mM, respectively. SyPyk2 is activated in the presence of glucose-6-phosphate (G6P) and ribose-5-phosphate (R5P); however, it remains unaltered in the presence of adenosine monophosphate (AMP) or fructose-1,6-bisphosphate. These results indicate that SyPyk2 is classified as PykA type rather than PykF, stimulated by sugar monophosphates, such as G6P and R5P, but not by AMP. SyPyk2, considering substrate affinity and effectors, can play pivotal roles in sugar catabolism under nonphotosynthetic conditions.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11101554/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140958706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional and regulatory diversity of homeobox-leucine zipper transcription factors BnaHB6 under dehydration and salt stress in Brassica napus L. 脱水和盐胁迫下甘蓝型油菜同源框-亮氨酸拉链转录因子 BnaHB6 的功能和调控多样性

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-05-15 DOI: 10.1007/s11103-024-01465-6

Natalia Żyła, Agata Cieśla, Laurencja Szała, Danuta Babula-Skowrońska

{"title":"Functional and regulatory diversity of homeobox-leucine zipper transcription factors BnaHB6 under dehydration and salt stress in Brassica napus L.","authors":"Natalia Żyła, Agata Cieśla, Laurencja Szała, Danuta Babula-Skowrońska","doi":"10.1007/s11103-024-01465-6","DOIUrl":"10.1007/s11103-024-01465-6","url":null,"abstract":"The plant-specific homeodomain-leucine zipper I subfamily is involved in the regulation of various biological processes, particularly growth, development and stress response. In the present study, we characterized four BnaHB6 homologues from Brassica napus. All BnaHB6 proteins have transcriptional activation activity. Structural and functional data indicate the complex role of BnaHB6 genes in regulating biological processes, with some functions conserved and others diverged. Transcriptional analyzes revealed that they are induced in a similar manner in different tissues but show different expression patterns in response to stress and circadian rhythm. Only the BnaA09HB6 and BnaC08HB6 genes are expressed under dehydration and salt stress, and in darkness. The partial transcriptional overlap of BnaHB6s with the evolutionarily related genes BnaHB5 and BnaHB16 was also observed. Transgenic Arabidopsis thaliana plants expressing a single proBnaHB6::GUS partially confirmed the expression results. Bioinformatic analysis allowed the identification of TF-binding sites in the BnaHB6 promoters that may control their expression under stress and circadian rhythm. ChIP-qPCR analysis revealed that BnaA09HB6 and BnaC08HB6 bind directly to the promoters of the target genes BnaABF4 and BnaDREB2A. Comparison of their expression patterns in the WT plants and the bnac08hb6 mutant showed that BnaC08HB6 positively regulates the expression of the BnaABF4 and BnaDREB2A genes under dehydration and salt stress. We conclude that four BnaHB6 homologues have distinct functions in response to stress despite high sequence similarity, possibly indicating different binding preferences with BnaABF4 and BnaDREB2A. We hypothesize that BnaC08HB6 and BnaA09HB6 function in a complex regulatory network under stress.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11096223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140945631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex. 拟南芥端粒酶 TERT-TR 复合物的特征。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-05-14 DOI: 10.1007/s11103-024-01461-w

Barbora Štefanovie, Leon P Jenner, Lucie Bozděchová, Petr Fajkus, Eva Sýkorová, Jiří Fajkus, Jan J Paleček

引用次数: 0

Molecular cloning and characterization of a salt overly sensitive3 (SOS3) gene from the halophyte Pongamia. 来自盐生植物 Pongamia 的盐过度敏感 3 (SOS3) 基因的分子克隆和特征描述。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-05-14 DOI: 10.1007/s11103-024-01459-4

Yi Zhang, Heng Yang, Yujuan Liu, Qiongzhao Hou, Shuguang Jian, Shulin Deng

{"title":"Molecular cloning and characterization of a salt overly sensitive3 (SOS3) gene from the halophyte Pongamia.","authors":"Yi Zhang, Heng Yang, Yujuan Liu, Qiongzhao Hou, Shuguang Jian, Shulin Deng","doi":"10.1007/s11103-024-01459-4","DOIUrl":"10.1007/s11103-024-01459-4","url":null,"abstract":"A high concentration of sodium (Na+) is the primary stressor for plants in high salinity environments. The Salt Overly Sensitive (SOS) pathway is one of the best-studied signal transduction pathways, which confers plants the ability to export too much Na+ out of the cells or translocate the cytoplasmic Na+ into the vacuole. In this study, the Salt Overly Sensitive3 (MpSOS3) gene from Pongamia (Millettia pinnata Syn. Pongamia pinnata), a semi-mangrove, was isolated and characterized. The MpSOS3 protein has canonical EF-hand motifs conserved in other calcium-binding proteins and an N-myristoylation signature sequence. The MpSOS3 gene was significantly induced by salt stress, especially in Pongamia roots. Expression of the wild-type MpSOS3 but not the mutated nonmyristoylated MpSOS3-G2A could rescue the salt-hypersensitive phenotype of the Arabidopsis sos3-1 mutant, which suggested the N-myristoylation signature sequence of MpSOS3 was required for MpSOS3 function in plant salt tolerance. Heterologous expression of MpSOS3 in Arabidopsis accumulated less H2O2, superoxide anion radical (O2-), and malondialdehyde (MDA) than wild-type plants, which enhanced the salt tolerance of transgenic Arabidopsis plants. Under salt stress, MpSOS3 transgenic plants accumulated a lower content of Na+ and a higher content of K+ than wild-type plants, which maintained a better K+/Na+ ratio in transgenic plants. Moreover, no development and growth discrepancies were observed in the MpSOS3 heterologous overexpression plants compared to wild-type plants. Our results demonstrated that the MpSOS3 pathway confers a conservative salt-tolerant role and provided a foundation for further study of the SOS pathway in Pongamia.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: variations and reduction of plastome are associated with the evolution of parasitism in Convolvulaceae. 更正：质体的变异和减少与旋花科寄生虫的进化有关。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-05-14 DOI: 10.1007/s11103-024-01464-7

Li-Qiong Chen, Xin Li, Xin Yao, De-Zhu Li, Craig Barrett, Claude W dePamphilis, Wen-Bin Yu

引用次数: 0

Transcriptome analysis and functional validation reveal the novel role of LhCYCL in axillary bud development in hybrid Liriodendron. 转录组分析和功能验证揭示了 LhCYCL 在杂交鹅掌楸腋芽发育过程中的新作用。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-05-10 DOI: 10.1007/s11103-024-01458-5

Shaoying Wen, Qinghua Hu, Jing Wang, Huogen Li

{"title":"Transcriptome analysis and functional validation reveal the novel role of LhCYCL in axillary bud development in hybrid Liriodendron.","authors":"Shaoying Wen, Qinghua Hu, Jing Wang, Huogen Li","doi":"10.1007/s11103-024-01458-5","DOIUrl":"10.1007/s11103-024-01458-5","url":null,"abstract":"Shoot branching significantly influences yield and timber quality in woody plants, with hybrid Liriodendron being particularly valuable due to its rapid growth. However, understanding of the mechanisms governing shoot branching in hybrid Liriodendron remains limited. In this study, we systematically examined axillary bud development using morphological and anatomical approaches and selected four distinct developmental stages for an extensive transcriptome analysis. A total of 9,449 differentially expressed genes have been identified, many of which are involved in plant hormone signal transduction pathways. Additionally, we identified several transcription factors downregulated during early axillary bud development, including a noteworthy gene annotated as CYC-like from the TCP TF family, which emerged as a strong candidate for modulating axillary bud development. Quantitative real-time polymerase chain reaction results confirmed the highest expression levels of LhCYCL in hybrid Liriodendron axillary buds, while histochemical β-glucuronidase staining suggested its potential role in Arabidopsis thaliana leaf axil development. Ectopic expression of LhCYCL in A. thaliana led to an increase of branches and a decrease of plant height, accompanied by altered expression of genes involved in the plant hormone signaling pathways. This indicates the involvement of LhCYCL in regulating shoot branching through plant hormone signaling pathways. In summary, our results emphasize the pivotal role played by LhCYCL in shoot branching, offering insights into the function of the CYC-like gene and establishing a robust foundation for further investigations into the molecular mechanisms governing axillary bud development in hybrid Liriodendron.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140899175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of CBL-CIPK signaling in plant responses to biotic and abiotic stresses. CBL-CIPK 信号在植物应对生物和非生物胁迫中的作用。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-05-07 DOI: 10.1007/s11103-024-01417-0

J S Chen, S T Wang, Q Mei, T Sun, J T Hu, G S Xiao, H Chen, Y H Xuan

引用次数: 0

ACRE, a class of AP2/ERF transcription factors, activates the expression of sweet potato ß-amylase and sporamin genes through the sugar-responsible element CMSRE-1. ACRE 是一类 AP2/ERF 转录因子，通过糖反应元件 CMSRE-1 激活甘薯 ß 淀粉酶和孢子粉基因的表达。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-05-07 DOI: 10.1007/s11103-024-01450-z

Kenichiro Maeo, Yuki Nakaya, Nobutaka Mitsuda, Sumie Ishiguro

{"title":"ACRE, a class of AP2/ERF transcription factors, activates the expression of sweet potato ß-amylase and sporamin genes through the sugar-responsible element CMSRE-1.","authors":"Kenichiro Maeo, Yuki Nakaya, Nobutaka Mitsuda, Sumie Ishiguro","doi":"10.1007/s11103-024-01450-z","DOIUrl":"10.1007/s11103-024-01450-z","url":null,"abstract":"Sugars, synthesized by photosynthesis in source organs, are loaded and utilized as an energy source and carbon skeleton in sink organs, and also known to be important signal molecules regulating gene expression in higher plants. The expression of genes coding for sporamin and β-amylase, the two most abundant proteins in storage roots of sweet potato, is coordinately induced by sugars. We previously reported on the identification of the carbohydrate metabolic signal-responsible element-1 (CMSRE-1) essential for the sugar-responsible expression of two genes. However, transcription factors that bind to this sequence have not been identified. In this study, we performed yeast one-hybrid screening using the sugar-responsible minimal promoter region of the ß-amylase gene as bait and a library composed only transcription factor cDNAs of Arabidopsis. Two clones, named Activator protein binding to CMSRE-1 (ACRE), encoding AP2/ERF transcription factors were isolated. ACRE showed transactivation activity of the sugar-responsible minimal promoter in a CMSRE-1-dependent manner in Arabidopsis protoplasts. Electric mobility shift assay (EMSA) using recombinant proteins and transient co-expression assay in Arabidopsis protoplasts revealed that ACRE could actually act to the CMSRE-1. Among the DEHYDRATION -RESPONSIVE ELEMENT BINDING FACTOR (DREB) subfamily, almost all homologs including ACRE, could act on the DRE, while only three ACREs could act to the CMSRE-1. Moreover, ACRE-homologs of Japanese morning glory also have the same property of DNA-binding preference and transactivation activity through the CMSRE-1. These findings suggested that ACRE plays an important role in the mechanism regulating the sugar-responsible gene expression through the CMSRE-1 conserved across plant species.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11076338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140876981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0