Plant Molecular Biology最新文献_第6页

Plant growth Enhancement in Colchicine-Treated Tomato Seeds without Polyploidy Induction. 秋水仙素处理过的番茄种子在不诱导多倍体的情况下提高了植株生长。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-12-12 DOI: 10.1007/s11103-024-01521-1

Rosa Irma Obando-González, Luis Enrique Martínez-Hernández, Leandro Alberto Núñez-Muñoz, Berenice Calderón-Pérez, Roberto Ruiz-Medrano, José Abrahán Ramírez-Pool, Beatriz Xoconostle-Cázares

{"title":"Plant growth Enhancement in Colchicine-Treated Tomato Seeds without Polyploidy Induction.","authors":"Rosa Irma Obando-González, Luis Enrique Martínez-Hernández, Leandro Alberto Núñez-Muñoz, Berenice Calderón-Pérez, Roberto Ruiz-Medrano, José Abrahán Ramírez-Pool, Beatriz Xoconostle-Cázares","doi":"10.1007/s11103-024-01521-1","DOIUrl":"10.1007/s11103-024-01521-1","url":null,"abstract":"Plant breeding plays a pivotal role in the development of improved tomato cultivars, addressing various challenges faced by this crop worldwide. Tomato crop yield is affected by biotic and abiotic stress, including diverse pathogens and pests, extreme temperatures, drought, and soil salinity, thus affecting fruit quality, and overall crop productivity. Through strategic plant breeding approaches, it is possible to increase the genetic diversity of tomato cultivars, leading to the development of varieties with increased resistance to prevalent diseases and pests, improved tolerance to environmental stress, and enhanced adaptability to changing agroclimatic conditions. The induction of genetic variability using antimitotic agents, such as colchicine, has been widely employed in plant breeding precisely to this end. In this study, we analyzed the transcriptome of colchicine-treated tomato plants exhibiting larger size, characterized by larger leaves, while seedlings of the T2 generation harbored three cotyledons. A total of 382 differentially expressed genes encoding proteins associated with anatomical structure development, hormone synthesis and transport, flavonoid biosynthesis, and responses to various stimuli, stresses, and defense mechanisms were identified. Gene enrichment analysis suggests a role for auxin and flavonoid biosynthesis in cotyledon formation. Furthermore, single-nucleotide polymorphisms were mapped in colchicine-treated plants and determined which corresponded to differentially- expressed genes. Interestingly, most were associated to only a few genes in a similar location. This study provides significant insights into the genes and metabolic pathways affected in colchicine-treated tomatoes that exhibit improved agronomic traits, such as plant vigor and improved photosynthesis rate.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 1","pages":"3"},"PeriodicalIF":3.9,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11638462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GhCNGC31 is critical for conferring resistance to Verticillium wilt in cotton. GhCNGC31是棉花抗黄萎病的关键基因。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-12-12 DOI: 10.1007/s11103-024-01533-x

Tianming Li, Wenjing Jia, Lin Li, Shi Xu, Ruqiang Xu

{"title":"GhCNGC31 is critical for conferring resistance to Verticillium wilt in cotton.","authors":"Tianming Li, Wenjing Jia, Lin Li, Shi Xu, Ruqiang Xu","doi":"10.1007/s11103-024-01533-x","DOIUrl":"10.1007/s11103-024-01533-x","url":null,"abstract":"In the past decades, cyclic nucleotide-gated ion channels (CNGCs) have been extensively studied in diploid species Arabidopsis thaliana. However, the functional diversification of CNGCs in crop plants, mostly polyploid, remains poorly understood. In allotetraploid Upland cotton (Gossypium hirsutum), GhCNGC31 is one of the multiple orthologs of AtCNGC2, being present in the plasma membrane, capable of interacting with itself and binding to calmodulins and cyclic nucleotides. GhCNGC31 knockdown plants exhibited slight growth inhibition, and became more susceptible to Verticillium dahliae infection, which was associated with the reduced lignin and flavonoid accumulation, impaired ROS (reactive oxygen species) burst, and down-regulation of defense-related genes PR1, JAZ2, LOX2, and RBOH10. RNA-Seq analysis identified 1817 differentially expressed genes from GhCNGC31 knockdown, of which 1184 (65%) were responsive to V. dahliae infection and accounted for 57% among a total of 2065 V. dahliae-responsive genes identified in this study. These GhCNGC31-regulated genes mainly function with cell wall organization and biogenesis, cellular carbohydrate metabolic or biosynthetic process, cellular component macromolecule biosynthetic process, and rhythmic process. They are significantly enriched in the pathways of plant MAPK signaling, plant-pathogen interaction, phenylpropanoid biosynthesis, and plant hormone signal transduction. A set of transcription factors (TFs) and resistance (R) genes are among the GhCNGC31-regulated genes, which are significantly over-represented with the TCP and WRKY TFs families, as well as with the R genes of T (TIR) and TNL (TIR-NB-LRR) classes. Together, our results unraveled a critical role of GhCNGC31 for conferring resistance to Verticillium wilt in cotton.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 1","pages":"2"},"PeriodicalIF":3.9,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 2-oxoglutarate-dependent dioxygenase, GLUCORAPHASATIN SYNTHASE 1 (GRS1) is a major determinant for different aliphatic glucosinolates between radish and Chinese cabbage. 2-氧戊二酸依赖的双加氧酶，GLUCORAPHASATIN SYNTHASE 1 （GRS1）是萝卜和白菜之间不同脂肪型硫代葡萄糖苷的主要决定因素。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-12-10 DOI: 10.1007/s11103-024-01537-7

Peter Choi, Adji Baskoro Dwi Nugroho, Heewon Moon, Dong-Hwan Kim

{"title":"A 2-oxoglutarate-dependent dioxygenase, GLUCORAPHASATIN SYNTHASE 1 (GRS1) is a major determinant for different aliphatic glucosinolates between radish and Chinese cabbage.","authors":"Peter Choi, Adji Baskoro Dwi Nugroho, Heewon Moon, Dong-Hwan Kim","doi":"10.1007/s11103-024-01537-7","DOIUrl":"10.1007/s11103-024-01537-7","url":null,"abstract":"Glucosinolates (GSLs) are secondary metabolites in Brassicaceae plants and play a defensive role against a variety of abiotic and biotic stresses. Also, it exhibits anti-cancer activity against cancer cell in human. Different profiles of aliphatic GSL compounds between radish and Chinese cabbage were previously reported. However, molecular details underlying the divergent profile between two species were not clearly understood. In this study, we found that major difference of aliphatic GSLs profiles between two species is determined by the dominantly expressed genes in first step of the secondary modification phase, which are responsible for enzymatic catalysis of methylthioalkyl-glucosinolate. For instance, active expression of GLUCORAPHASATIN SYNTHASE 1 (GRS1) gene in radish play an important role in the production of glucoraphasatin (GRH) and glucoraphenin (GRE), a major aliphatic GSLs in radish. Meanwhile, Chinese cabbage was found to merely produce glucoraphasatin (GRH), instead producing glucoraphanin (GRA) and gluconapin (GNP) due to the mere expression of GRS1 homologs and abundant expressions of FLAVIN-CONTAINING MONOOXYGENASES (FMO GS-OX) homologs in Chinese cabbage. In addition, we noticed that wounding treatment on leaf tissues substantially enhanced the production of aliphatic and benzenic GSLs in both Chinese cabbage and radish, indicating that GSLs are wound-induced defensive compounds in both Chinese cabbage and radish plants.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 1","pages":"1"},"PeriodicalIF":3.9,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogen peroxide priming triggers splicing memory in grape berries. 过氧化氢引物触发葡萄浆果中的拼接记忆。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-11-28 DOI: 10.1007/s11103-024-01528-8

Ding-Ding Zuo, Hao-Ting Sun, Lu Yang, Meng-Ling Zheng, Jing Zhang, Da-Long Guo

{"title":"Hydrogen peroxide priming triggers splicing memory in grape berries.","authors":"Ding-Ding Zuo, Hao-Ting Sun, Lu Yang, Meng-Ling Zheng, Jing Zhang, Da-Long Guo","doi":"10.1007/s11103-024-01528-8","DOIUrl":"10.1007/s11103-024-01528-8","url":null,"abstract":"Plants are highly sensitive to environmental changes, and alternative splicing (AS) has been described in many studies due to its important control role in stress response. Recent studies indicated that plants exhibit splicing memory to stress to effectively activate transcriptional adaptation. Hydrogen peroxide (H2O2), as a reactive oxygen species (ROS), has toxic effects on plants. However, it also has a significant effect on promoting early maturity of 'Kyoho' grape at low concentrations. To explore the mechanism of priming treatment of H2O2 showing better promotion effect, the RNA-Seq data of H2O2-primied and no-primied fruits were analyzed. The genes with H2O2 stress splicing memory were identified, accompanied by changes in H3K4me3 modification levels, and their splicing memory patterns were verified by PCR and agarose gel electrophoresis. This finding establishes a link between alternative splicing memory and fruit ripening under H2O2 regulation and contribute to develop the application of H2O2 in fruit ripening.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"114 6","pages":"129"},"PeriodicalIF":3.9,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142740177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolite and transcriptome reveal the lipid-associated key components and genes regulated by BoORP3a in ornamental kale. 代谢物和转录组揭示了观赏甘蓝中与脂质相关的关键成分和受 BoORP3a 调控的基因。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-11-28 DOI: 10.1007/s11103-024-01524-y

Zheng Liu, Fuhui Zhou, Yashu Li, Xin Feng, Pengfang Zhu

{"title":"Metabolite and transcriptome reveal the lipid-associated key components and genes regulated by BoORP3a in ornamental kale.","authors":"Zheng Liu, Fuhui Zhou, Yashu Li, Xin Feng, Pengfang Zhu","doi":"10.1007/s11103-024-01524-y","DOIUrl":"10.1007/s11103-024-01524-y","url":null,"abstract":"BoORP3a, an oxysterol-binding protein, located in the endoplasmic reticulum (ER), may function in cuticular wax deposition in ornamental kale. In this study, we investigated its regulation of the key components of cuticular wax and lipids, metabolic pathways, and potential target genes. HS-SPME/GC-MS identified 34 and 31 volatile organic compounds in wild-type and the BoORP3a-overexpressing plant OE-ORP3a-7, respectively, primarily including alkane, ketone, ester, and alcohol. Hentriacontane, 15-nonacosanone, and > C20 alkanes were more abundant in OE-ORP3a-7, which may result in more cuticular wax in this plant. RNA sequencing identified 223 differentially expressed genes (DEGs) between wild-type and OE-ORP3a-7, comprising 119 upregulated and 104 downregulated DEGs. The KEGG enrichment analysis revealed that the downregulated DEGs in OE-ORP3a-7 were involved in glyoxylate and dicarboxylate metabolism, SNARE (Soluble N-ethylmaleimide-sensitive factor attachment protein receptor) interactions in vesicular transport, fatty acid biosynthesis, and glycerolipid metabolism; the upregulated DEGs were involved in steroid biosynthesis, fatty acid degradation, alpha-linolenic acid metabolism, and sphingolipid metabolism. Bo1g106990, Bo1g123670, and Bo9g166090 were identified as key DEGs in lipid-related pathways. We speculate that BoORP3a regulates several lipid metabolisms and may coordinate lipid turnover and remodeling. The results of this study will enrich the functionality of the ORPs family, provide new insights into plant wax research, and have significant implications for ornamental kale breeding.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"114 6","pages":"130"},"PeriodicalIF":3.9,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142740179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms of plant productivity enhancement by nano fertilizers for sustainable agriculture. 纳米肥料提高植物生产力的分子机制，促进可持续农业。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-11-26 DOI: 10.1007/s11103-024-01527-9

Arpan Dey, Ayan Sadhukhan

{"title":"Molecular mechanisms of plant productivity enhancement by nano fertilizers for sustainable agriculture.","authors":"Arpan Dey, Ayan Sadhukhan","doi":"10.1007/s11103-024-01527-9","DOIUrl":"10.1007/s11103-024-01527-9","url":null,"abstract":"Essential plant nutrients encapsulated or combined with nano-dimensional adsorbents define nano fertilizers (NFs). Nanoformulation of non-essential elements enhancing plant growth and stress tolerance also comes under the umbrella of NFs. NFs have an edge over conventional chemical fertilizers, viz., higher plant biomass and yield using much lesser fertilization, thereby reducing environmental pollution. Foliar and root applications of NFs lead to their successful uptake by the plant, depending on the size, surface charge, and other physicochemical properties of NFs. Smaller NFs can pass through channels on the waxy cuticle depending on the hydrophobicity, while larger NFs pass through the stomatal conduits of leaves. Charge-based adsorption, followed by apoplastic movement and endocytosis, translocates NFs through the root, while the size of NFs influences passage into vascular tissues. Recent transcriptomic, proteomic, and metabolomic studies throw light on the molecular mechanisms of growth promotion by NFs. The expression levels of nutrient transporter genes are regulated by NFs, controlling uptake and minimizing excess nutrient toxicity. Accelerated growth by NFs is brought about by their extensive regulation of cell division, photosynthesis, carbohydrate, and nitrogen metabolism, as well as the phytohormone-dependent signaling pathways related to development, stress response, and plant defense. NFs mimic Ca,2+ eliciting second messengers and associated proteins in signaling cascades, reaching transcription factors and finally orchestrating gene expression to enhance growth and stress tolerance. Developing advanced nano fertilizers of the future must involve exploring molecular interactions with plants to reduce toxicity and improve effectiveness.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"114 6","pages":"128"},"PeriodicalIF":3.9,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel QTL qRYM-7H for barley yellow mosaic resistance identified by GWAS and linkage analysis. 通过 GWAS 和关联分析发现的大麦黄镶嵌抗性新 QTL qRYM-7H

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-11-22 DOI: 10.1007/s11103-024-01529-7

Juan Zhu, Hui Zhou, Mengna Zhang, Yi Hong, Yuhang Zhang, Chao Lv, Baojian Guo, Feifei Wang, Rugen Xu

{"title":"A novel QTL qRYM-7H for barley yellow mosaic resistance identified by GWAS and linkage analysis.","authors":"Juan Zhu, Hui Zhou, Mengna Zhang, Yi Hong, Yuhang Zhang, Chao Lv, Baojian Guo, Feifei Wang, Rugen Xu","doi":"10.1007/s11103-024-01529-7","DOIUrl":"10.1007/s11103-024-01529-7","url":null,"abstract":"Barley (Hordeum vulgare L.) is the fourth largest cereal crop in the world after rice, wheat and maize. Barley yellow mosaic disease (BYMD) is a serious threat to winter barley production. The evolution and mutation of virus strains lead to the breakdown of the resistance of the originally resistant varieties. It is therefore vital to explore new BYMD resistance genes. In this study, a natural population (334 barley varieties or lines) and a double haploid population derived from the cross between Tam407227 and Franklin were used to search for new quantitative trait loci (QTL) for BYMD resistance. Two major QTL on chromosomes 3H and 7H, respectively, were detected from the genome wide association study and validated in the DH population. Among them, The QTL on 3H (qRYM-3H/qTFRYM-3H) was confirmed to be the reported BYMD resistance gene eIF4E by haplotype analysis. And the QTL on 7H (qRYM-7H/qTFRYM-7H) is a novel QTL that has not been reported before. Another QTL on 2H was identified from the DH population. This QTL is more likely the Rmy16Hb reported previously. These three QTL showed an additive effect on improving BYMD resistance with the average disease scores from 2.45 (all sensitive alleles for these three QTL) to 0.62 (all tolerant alleles for these three QTL). The candidate genes for the novel QTL qRYM-7H/qTFRYM-7H were predicted based on transcriptome sequencing and qPCR analysis.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"114 6","pages":"127"},"PeriodicalIF":3.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic and metabolomic insights into the mechanisms of calcium-mediated salt stress tolerance in hemp. 蛋白质组学和代谢组学对大麻钙介导的盐胁迫耐受机制的启示。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-11-18 DOI: 10.1007/s11103-024-01525-x

Yang Yang, Zhenhua Lu, Hailong Ye, Jiafeng Li, Yan Zhou, Ling Zhang, Gang Deng, Zheng Li

{"title":"Proteomic and metabolomic insights into the mechanisms of calcium-mediated salt stress tolerance in hemp.","authors":"Yang Yang, Zhenhua Lu, Hailong Ye, Jiafeng Li, Yan Zhou, Ling Zhang, Gang Deng, Zheng Li","doi":"10.1007/s11103-024-01525-x","DOIUrl":"10.1007/s11103-024-01525-x","url":null,"abstract":"Industrial hemp (Cannabis sativa L.) is a multifaced crop that has the potential to be exploited for many industrial applications, and making use of salt lands is considered to be a sustainable development strategy for the hemp industry. However, no elite salt-tolerant hemp varieties have been developed, and therefore supplementing appropriate exogenous substances to saline soil is one possible solution. Calcium-containing compounds are well-known for their salt tolerance enhancing effects, but the underlying molecular mechanisms remain largely unclear. Here, we first assessed the ameliorative effects of calcium amendments on salt-stressed hemp plants and then investigated these mechanisms on hemp using integrative analysis of proteomics and metabolomics. The stress phenotypes could be lessened by Ca2+ treatment. Certain concentrations of Ca2+ maintained relative electrical conductivity and the contents of malondialdehyde and chlorophyll. Ca2+ treatment also generally led to greater accumulations of soluble proteins, soluble carbohydrates and proline, and enhanced the activities of superoxide dismutase and peroxidase. Through functional classification, pathway enrichment, and network analysis, our data reveal that accumulation of dipeptides is a prominent metabolic signature upon exogenous Ca2+ treatment, and that changes in mitochondrial properties may play an important role in enhancing the salt tolerance. Our results outline the complex metabolic alternations involved in calcium-mediated salt stress resistance, and these data and analyses would be useful for future functional studies.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"114 6","pages":"126"},"PeriodicalIF":3.9,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Publisher Correction: Alternative splicing and deletion in S-RNase confer stylar-part self-compatibility in the apple cultivar 'Vered'. 出版者更正：苹果栽培品种 "Vered "中 S-RNase 的替代剪接和缺失赋予花柱部分自相容性。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-11-13 DOI: 10.1007/s11103-024-01526-w

Kazuma Okada, Taku Shimizu, Shigeki Moriya, Masato Wada, Kazuyuki Abe, Yutaka Sawamura

引用次数: 0

Multi-omics analysis reveals the positive impact of differential chloroplast activity during in vitro regeneration of barley. 多组学分析揭示了大麦离体再生过程中叶绿体活动差异的积极影响。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2024-11-13 DOI: 10.1007/s11103-024-01517-x

Parul Sirohi, Chanderkant Chaudhary, Mayank Sharma, Ravi Bodampalli Anjanappa, Suchi Baliyan, Ritika Vishnoi, Sumit Kumar Mishra, Reeku Chaudhary, Bhairavnath Waghmode, Anuj Kumar Poonia, Hugo Germain, Debabrata Sircar, Harsh Chauhan

{"title":"Multi-omics analysis reveals the positive impact of differential chloroplast activity during in vitro regeneration of barley.","authors":"Parul Sirohi, Chanderkant Chaudhary, Mayank Sharma, Ravi Bodampalli Anjanappa, Suchi Baliyan, Ritika Vishnoi, Sumit Kumar Mishra, Reeku Chaudhary, Bhairavnath Waghmode, Anuj Kumar Poonia, Hugo Germain, Debabrata Sircar, Harsh Chauhan","doi":"10.1007/s11103-024-01517-x","DOIUrl":"10.1007/s11103-024-01517-x","url":null,"abstract":"Existence of potent in vitro regeneration system is a prerequisite for efficient genetic transformation and functional genomics of crop plants. In this study, two contrasting cultivars differencing in their in vitro regeneration efficiency were identified. Tissue culture friendly cultivar Golden Promise (GP) and tissue culture resistant DWRB91(D91) were selected as contrasting cultivars to investigate the molecular basis of regeneration efficiency through multiomics analysis. Transcriptomics analysis revealed 1487 differentially expressed genes (DEGs), in which 795 DEGs were upregulated and 692 DEGs were downregulated in the GP-D91 transcriptome. Genes encoding proteins localized in chloroplast and involved in ROS generation were upregulated in the embryogenic calli of GP. Moreover, proteome analysis by LC-MS/MS revealed 3062 protein groups and 16,989 peptide groups, out of these 1586 protein groups were differentially expressed proteins (DEPs). Eventually, GC-MS based metabolomics analysis revealed the higher activity of plastids and alterations in key metabolic processes such as sugar metabolism, fatty acid biosynthesis, and secondary metabolism. TEM analysis also revealed differential plastid development. Higher accumulation of sugars, amino acids and metabolites corresponding to lignin biosynthesis were observed in GP as compared to D91. A comprehensive examination of gene expression, protein profiling and metabolite patterns unveiled a significant increase in the genes encompassing various functions, such as ion homeostasis, chlorophyll metabolic process, ROS regulation, and the secondary metabolic pathway.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"114 6","pages":"124"},"PeriodicalIF":3.9,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0