Physiology international最新文献_第3页

Beyond the acute illness: Exploring long COVID and its impact on multiple organ systems. 超越急性疾病：探索长期新冠肺炎及其对多器官系统的影响。

IF 1.4 4区医学

Physiology international Pub Date : 2023-11-09 Print Date: 2023-12-18 DOI: 10.1556/2060.2023.00256

Nandini Bhattacharjee, Parantap Sarkar, Tania Sarkar

{"title":"Beyond the acute illness: Exploring long COVID and its impact on multiple organ systems.","authors":"Nandini Bhattacharjee, Parantap Sarkar, Tania Sarkar","doi":"10.1556/2060.2023.00256","DOIUrl":"10.1556/2060.2023.00256","url":null,"abstract":"Unprecedented worldwide health catastrophe due to the COVID-19 pandemic has ended up resulting in high morbidity and mortality rates. Even though many people recover from acute infection, there is rising concern regarding post-COVID-19 conditions (PCCs), often referred to as post-acute sequelae of SARS-CoV-2 infection (PASC) or \"long COVID.\" The respiratory, cardiovascular, neurological, and endocrine systems are just a few of the many organ systems that can be impacted by this multifarious, complicated illness. The clinical manifestations of long COVID can vary among individuals and may include fatigue, dyspnea, chest pain, cognitive impairment, and new-onset diabetes, among others. Although the underlying processes of long COVID are not fully understood, they probably involve unregulated immune response, persistent generation of pro-inflammatory cytokines (chronic inflammation), autoimmune-like reactions, persistent viral replication, and micro-clot formation. To create successful treatments and care plans, it is essential to comprehend the immunological mechanisms causing these difficulties. The pathogenesis of long COVID should be clarified and potential biomarkers to help with diagnosis and treatment should be sought after. To reduce the burden of long COVID on people and healthcare systems around the world, the need for long-term monitoring and management of long COVID problems should be emphasized. It also underscores the significance of a multidisciplinary approach to patient care. The goal of this review is to carefully evaluate the clinical signs and symptoms of long COVID, their underlying causes, and any potential immunological implications.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71522261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATXN3 promotes proliferation, stemness and motility of clear cell renal cell carcinoma cells by regulating S100A8 ubiquitination. ATXN3通过调节S100A8泛素化促进透明细胞肾癌细胞的增殖、干性和运动性。

IF 1.4 4区医学

Physiology international Pub Date : 2023-11-08 Print Date: 2023-12-18 DOI: 10.1556/2060.2023.00247

Jixiang Bai, Jieru Han, Jiayi Fan, Jing Song, Shuhui Wang

{"title":"ATXN3 promotes proliferation, stemness and motility of clear cell renal cell carcinoma cells by regulating S100A8 ubiquitination.","authors":"Jixiang Bai, Jieru Han, Jiayi Fan, Jing Song, Shuhui Wang","doi":"10.1556/2060.2023.00247","DOIUrl":"10.1556/2060.2023.00247","url":null,"abstract":"Background: Clear cell renal cell carcinoma (ccRCC) is a dominant subtype of kidney cancer with a dismal outcome at advanced stages. Ataxin 3 (ATXN3) has been proven to play a cancer-promoting role in several tumors and is upregulated in the patients with renal cell carcinoma. Thus, the objective of this research is to examine the biological roles and underlying mechanisms of ATXN3 in ccRCC.Methods: Bioinformatics analysis was carried out to analyze ATXN3 expression in ccRCC tissues and patient survival. Gain- and loss-of-function assays were applied to explore the effect of ATXN3 on ccRCC cell malignant behavior in vitro. The effect of ATXN3 on the NF-κB pathway was assessed by Western blot and immunofluorescence staining. The binding between ATXN3 and S100A8 and the effect of ATXN3 on S100A8 ubiquitination were verified using coimmunoprecipitation.Results: ATXN3 was upregulated in ccRCC tissues and correlated with adverse patient outcome. ATXN3 overexpression facilitated the proliferation, stemness, invasion and migratory capacity of ccRCC cells, whereas silencing had the opposite effect. ATXN3 enhanced the activity of the NF-κB pathway. Silencing ATXN3 facilitated S100A8 ubiquitination. Rescue experiments demonstrated that S100A8 downregulation reversed the promoting effect of ATXN3 on malignant behavior and NF-κB pathway activation in ccRCC cells.Conclusion: ATXN3 exerts a cancer-promoting effect in ccRCC by regulating S100A8 ubiquitination. Therefore, targeting the ATXN3/S100A8/NF-κB axis may provide a novel underlying therapeutic strategy for ccRCC.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71484842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stent composition and immune response after long-term coronary angioplasty. 支架成分与长期冠状动脉血管成形术后的免疫反应。

IF 1.4 4区医学

Physiology international Pub Date : 2023-11-07 Print Date: 2023-12-18 DOI: 10.1556/2060.2023.00162

Viviane A R Sant'Anna, Adriano H P Barbosa, Rodrigo A Souza, José M A Sousa, Frederico Monfardini, Magnus Gidlund, Henrique A R Fonseca

{"title":"Stent composition and immune response after long-term coronary angioplasty.","authors":"Viviane A R Sant'Anna, Adriano H P Barbosa, Rodrigo A Souza, José M A Sousa, Frederico Monfardini, Magnus Gidlund, Henrique A R Fonseca","doi":"10.1556/2060.2023.00162","DOIUrl":"10.1556/2060.2023.00162","url":null,"abstract":"Background: There are limited data about the influence of stent composition on immune responses after percutaneous coronary intervention (PCI).Objective: The aim was to compare the effects of PCI with conventional cobalt-chromium bare metal stent (BMS) and drug-eluting stent (DES) implantation on the modulation of humoral and cellular immune responses.Methods: A randomised, single-centre, open pilot study involving patients with stable coronary artery disease eligible for PCI was performed. Blood samples were collected from the peripheral artery (PA) and the coronary sinus (CS) at baseline and 40 weeks following PCI. IgM and IgG autoantibodies (Abs), anti-oxLDL and anti-ApoB-D, as well as cytokine levels were evaluated by enzyme-linked immunosorbent assay.Results: A total of 30 patients of 60 years mean age were included, 68% of whom were men. At the nine-month follow-up, a modulation in the levels of cytokines and autoantibodies was observed in both stent type groups. However, no difference was observed in the modulation of these markers between stents.Conclusion: The stent type promotes modulations in cellular and humoral immune responses in the long-term, with differences in the magnitude of effects in specific immune responses.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71484843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effectiveness of an mHealth intervention on diabetes risk factors and body composition 移动健康干预对糖尿病危险因素和身体成分的有效性

4区医学

Physiology international Pub Date : 2023-10-12 DOI: 10.1556/2060.2023.00209

Georgina Simkó, Martina Uvacsek

{"title":"The effectiveness of an mHealth intervention on diabetes risk factors and body composition","authors":"Georgina Simkó, Martina Uvacsek","doi":"10.1556/2060.2023.00209","DOIUrl":"https://doi.org/10.1556/2060.2023.00209","url":null,"abstract":"Abstract Early identification and lifestyle intervention is beneficial for people with risk for diabetes. The aim of this study was to evaluate the risk of type 2 diabetes (T2D) in healthy overweight or obese women using the FINDRISC score and a twelve-week long mobile app-based lifestyle intervention. Fifty-four subjects were involved and forty-eight were analysed, n = 28 in the intervention group (online group: OG) and n = 20 in the control group (CG). Body composition was assessed using the InBody 720 device and diabetes risk was evaluated with the Finnish Diabetes Risk Questionnaire. The calorie intake and macronutrients were evaluated by a 3-day diary. Group differences and pre- and post-results were analysed using student t -tests by TIBCO Statistica 13.40.14. Significantly decreased body mass index (28.0 ± 2.5 kg m −2 vs 25.8 ± 4.3 kg m −2 , P = 0.00), body fat percentage (37.5 ± 6.3% vs 34.1 ± 5.9%, P = 0.03), waist circumference (100.8 ± 7.2 cm vs 94.7 ± 8.2 cm, P = 0.00) and visceral fat (124.0 ± 29.2 cm³ vs 109.0 ± 24.6 cm³, P = 0.04) were found in the OG. By the end of the programme, both groups showed significant decrease in food consumption, daily calorie intake (OG: 2,348.6 ± 348.0 vs 1,483.1 ± 114.4, CG: 2,372.4 ± 464.2 vs 1,654.1 ± 201.3 kcal day −1 , P = 0.00), fibre (OG: 19.5 ± 3.7 vs 26.1 ± 3.4, CG: 17.8 ± 3.9 vs 22.0 ± 4.8 g day −1 , P = 0.00) and cholesterol consumption (OG: 365.2 ± 58.9 vs 266.2 ± 65.8, CG: 377.4 ± 72.1 vs 269.2 ± 42.7 mg day −1 , P = 0.00). OG had a more significant reduction in body mass index ( P = 0.03) and body fat percentage ( P = 0.04) values at the end of the programme compared to the control group. In this study, it was found that mHealth intervention is a useful and effective method in the Hungarian female population. Further studies are needed to investigate modifications of this intervention to achieve more health-related effects.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136013311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Academic stress, hair and saliva cortisol, and their relationship with body mass index and fat percentage in first year medical students. 一年级医学生学业压力、毛发和唾液皮质醇及其与体重指数和脂肪率的关系

IF 1.4 4区医学

Physiology international Pub Date : 2023-09-05 DOI: 10.1556/2060.2023.00178

Emilio Romero-Romero, Estela Guerreo De León, Juan Morán-Pinzón, Rigoberto Salado-Castillo, Armando Castillo-Pimentel

{"title":"Academic stress, hair and saliva cortisol, and their relationship with body mass index and fat percentage in first year medical students.","authors":"Emilio Romero-Romero, Estela Guerreo De León, Juan Morán-Pinzón, Rigoberto Salado-Castillo, Armando Castillo-Pimentel","doi":"10.1556/2060.2023.00178","DOIUrl":"https://doi.org/10.1556/2060.2023.00178","url":null,"abstract":"Introduction\u0000Although a large part of the population may be exposed to various pressures that can lead to mental or eating problems and increased perceived stress, the transition from adolescence to adulthood has been shown to be a crucial stage. Medical students are particularly vulnerable during the transition period as they must adapt to new circumstances, which may contribute to increased perceived stress. Cortisol plays an important role between stress, weight gain, and the development of obesity. We designed a study to investigate the association between stress, eating behaviour, cortisol, and body weight in a sample of first-year medical students.\u0000\u0000\u0000Methods\u0000We determined 75 first-year medical students' hair and salivary cortisol concentrations by ELISA and related it to self-reported stress, eating behaviour, and anthropometric measurements throughout the academic period. The prevalence of overweight and obesity in females was 25% and 10%, and in males was 35% and 6%, respectively. We report an increase in hair cortisol, higher self-reported stress scores, and BMI mainly in females. Finally, we found evidence of positive associations between hair cortisol and BMI in females (r = 0.348) and males (r = 0.423).\u0000\u0000\u0000Conclusion\u0000There is a low association between short-term single-point cortisol measures and long-term cortisol, mainly in males. Hence, short-term cortisol reactivity is moderately associated with long-term cortisol reactivity when both are evaluated simultaneously. These results support the previous evidence of positive associations between cortisol with body fat percentage and BMI, and finally, that eating behaviours are modified by academic stress perception, mainly in females.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10532319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Hormonal changes in the first 24 postoperative hours after cardiac surgical procedures. 心脏外科手术后24小时内的激素变化。

IF 1.4 4区医学

Physiology international Pub Date : 2023-09-05 DOI: 10.1556/2060.2023.00219

Balázs Szécsi, Krisztina Tóth, András Szabó, Csaba Eke, Rita Szentgróti, Orsolya Dohán, Kálmán Benke, Tamás Radovits, Miklós Pólos, Béla Merkely, János Gál, Andrea Székely

{"title":"Hormonal changes in the first 24 postoperative hours after cardiac surgical procedures.","authors":"Balázs Szécsi, Krisztina Tóth, András Szabó, Csaba Eke, Rita Szentgróti, Orsolya Dohán, Kálmán Benke, Tamás Radovits, Miklós Pólos, Béla Merkely, János Gál, Andrea Székely","doi":"10.1556/2060.2023.00219","DOIUrl":"https://doi.org/10.1556/2060.2023.00219","url":null,"abstract":"Background: Hormone level changes after heart surgeries are a widely observed phenomenon due to neurohormonal feedback mechanisms that may affect postoperative morbidity and mortality. The current study aimed to analyze the changes in thyroid and sex hormones in the first 24 postoperative hours after heart surgery.Methods: This prospective, observational study (registered on ClinicalTrials.gov: NCT03736499; 09/11/2018) included 49 patients who underwent elective cardiac surgical procedures at a tertiary heart center between March 2019 and December 2019. Thyroid hormones, including thyroid-stimulating hormone (TSH), triiodothyronine (T3), and thyroxine (T4), and sex hormones, including prolactin (PRL) and total testosterone, were measured preoperatively and at 24 h postoperatively.Results: Significant decreases in serum TSH (P < 0.001), T3 (P < 0.001) and total testosterone (P < 0.001) levels were noted, whereas T4 (P = 0.554) and PRL (P = 0.616) did not significantly change. Intensive care unit (ICU) hours (P < 0.001), mechanical ventilation (P < 0.001) and Vasoactive-Inotropic Score (VIS) (P = 0.006) were associated with postoperative T3 level. ICU hours were associated with postoperative T4 level (P = 0.028). Postoperative and delta testosterone levels were in connection with lengths of stay in ICU (P = 0.032, P = 0.010 respectively). Model for End-Stage Liver Disease (MELD) scores were associated with thyroid hormone levels and serum testosterone.Conclusions: T3 may represent a marker of nonthyroidal illness syndrome and testosterone may reflect hepatic dysfunction. In addition, PRL may act as a stress hormone in female patients.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10161914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors improving mortality in critically ill patients with liver failure - A systematic review. 提高肝功能衰竭危重患者死亡率的因素——系统综述。

IF 1.4 4区医学

Physiology international Pub Date : 2023-09-05 DOI: 10.1556/2060.2023.00211

Enikő Kovács, Nicolò Maimeri, Filippo Orlando, Federica Morselli, Ottavia Pallanch, Monica Fedrizzi, János Gál, Andrea Székely

{"title":"Factors improving mortality in critically ill patients with liver failure - A systematic review.","authors":"Enikő Kovács, Nicolò Maimeri, Filippo Orlando, Federica Morselli, Ottavia Pallanch, Monica Fedrizzi, János Gál, Andrea Székely","doi":"10.1556/2060.2023.00211","DOIUrl":"https://doi.org/10.1556/2060.2023.00211","url":null,"abstract":"Background: Acute and chronic hepatic failure can lead to increased mortality in critically ill and perioperative patients. Understanding the pathophysiological principles of these conditions in critically ill patients is of great importance to reduce mortality. The aim of our systematic literature review was to identify all randomized controlled trials on any intervention that had a statistically significant documented reduction in mortality in patients with hepatic failure.Methods: We searched PubMed, Scopus and Embase databases for pertinent studies on January 1st 2021. The following studies were included: randomized controlled trials; studies investigating adult critically ill or perioperative patient populations with any form of hepatic failure; mortality as primary or secondary outcome; and statistically significant differences in mortality between the examined groups.Results: We finally found nine trials in our systematic review on the effect of antibiotic administration and infectious diseases among patients with cirrhosis (three studies); immune modulation after liver transplantation (one study); administration of colloids in cirrhotic patients (one study); the effect of high-volume plasma exchange in acute liver failure (one study); administration of N-acetylcysteine in acute liver failure (one study); and treatment with terlipressin (two studies).Conclusion: In the present review we found only nine randomized studies with a documented survival benefit in patients with liver failure. Strategies that most improved mortality were associated with the outcome of sepsis and renal function.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10157656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Longitudinal changes of grip strength and forearm muscle thickness in young children. 幼儿握力和前臂肌肉厚度的纵向变化。

IF 1.4 4区医学

Physiology international Pub Date : 2023-09-05 DOI: 10.1556/2060.2023.00188

Takashi Abe, Hayao Ozaki, Akemi Abe, Shuichi Machida, Hisashi Naito, Jeremy P Loenneke

{"title":"Longitudinal changes of grip strength and forearm muscle thickness in young children.","authors":"Takashi Abe, Hayao Ozaki, Akemi Abe, Shuichi Machida, Hisashi Naito, Jeremy P Loenneke","doi":"10.1556/2060.2023.00188","DOIUrl":"https://doi.org/10.1556/2060.2023.00188","url":null,"abstract":"Background: Grip strength is a marker of future health conditions and is mainly generated by the extrinsic flexor muscles of the fingers. Therefore, whether or not there is a relationship between grip strength and forearm muscle size is vital in considering strategies for grip strength development during growth. Thus, this study aimed to examine the association between changes in grip strength and forearm muscle thickness in young children.Methods: Two hundred eighteen young children (104 boys and 114 girls) performed maximum voluntary grip strength and ultrasound-measured muscle thickness measurements in the right hand. Two muscle thicknesses were measured as the perpendicular distance between the adipose tissue-muscle interface and muscle-bone interface of the radius (MT-radius) and ulna (MT-ulna). All participants completed the first measurement and underwent a second measurement one year after the first one.Results: There were significant (P < 0.001) within-subject correlations between MT-ulna and grip strength [r = 0.50 (0.40, 0.60)] and MT-radius and grip strength [r = 0.59 (0.49, 0.67)]. There was no significant between-subject correlation between MT-ulna and grip strength [r = 0.07 (-0.05, 0.20)], but there was a statistically significant (P < 0.001) between-subject relationship between MT-radius and grip strength [r = 0.27 (0.14, 0.39)].Conclusion: Although we cannot infer causation from the present study, our findings suggest that as muscle size increases within a child, so does muscle strength. Our between-subject analysis, however, suggests that those who observed the greatest change in muscle size did not necessarily get the strongest.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10158825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

BCR::ABL1 negative myeloproliferative neoplasms: A review focused on essential thrombocythemia and polycythemia vera. ABL1阴性骨髓增生性肿瘤:原发性血小板增多症和真性红细胞增多症的研究进展

IF 1.4 4区医学

Physiology international Pub Date : 2023-09-05 DOI: 10.1556/2060.2023.00261

Malaz Khodier, Klára Gadó

{"title":"BCR::ABL1 negative myeloproliferative neoplasms: A review focused on essential thrombocythemia and polycythemia vera.","authors":"Malaz Khodier, Klára Gadó","doi":"10.1556/2060.2023.00261","DOIUrl":"https://doi.org/10.1556/2060.2023.00261","url":null,"abstract":"The classical myeloproliferative neoplasms are divided into chronic myeloid leukemia, and the Philadelphia negative polycythemia vera, essential thrombocythemia and primary myelofibrosis. These are heterogenous diseases, originating from the clonal proliferation of myeloid stem cells, resulting in increased mature cell numbers in one or more myeloid lineages. The most commonly seen mutations in the Philadelphia negative myeloproliferative neoplasms include those in Janus kinase, myeloproliferative leukemia protein and the calreticulin genes. Philadelphia negative myeloproliferative neoplasms occur infrequently, with a combined annual incidence of 2.58 per 100,000. There are many overlapping symptoms of Philadelphia negative MPNs, such as fatigue, night sweats, hepatosplenomegaly and circulatory symptoms due to increased cell numbers. Total Symptom Score of the MPN Symptom Assessment Form is used to assess symptom burden on patients. The most worrisome complications are thrombo-hemorrhagic events, and risk stratification is especially important as treatment of disease is based on their category. Phlebotomy and aspirin are the mainstay of treatment in low-risk polycythemia vera and essential thrombocythemia patients, whereas high-risk disease calls for additional cytoreduction, usually with hydroxyurea.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10532318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidized LDL-mediated upregulation of ADAMTS-4 in monocytes/macrophages involves ROS-NF-κB-SIRT-1 pathway. 氧化ldl介导的单核/巨噬细胞ADAMTS-4上调涉及ROS-NF-κB-SIRT-1通路。

IF 1.4 4区医学

Physiology international Pub Date : 2023-06-12 DOI: 10.1556/2060.2023.00170

Sukumaran Sreedevi Aswani, Mithra Sudha Mohan, Nandakumaran Sakunthala Aparna, Puthenpura Thankappan Boban, Kamalamma Saja

{"title":"Oxidized LDL-mediated upregulation of ADAMTS-4 in monocytes/macrophages involves ROS-NF-κB-SIRT-1 pathway.","authors":"Sukumaran Sreedevi Aswani, Mithra Sudha Mohan, Nandakumaran Sakunthala Aparna, Puthenpura Thankappan Boban, Kamalamma Saja","doi":"10.1556/2060.2023.00170","DOIUrl":"https://doi.org/10.1556/2060.2023.00170","url":null,"abstract":"Background and aims: ADAMTS-4 is a protease enzyme involved in vascular remodeling and atherosclerosis. It was found to be upregulated in macrophages seen in atherosclerotic lesions. This study aimed to investigate the expression and regulation of ADAMTS-4 in oxidized LDL-induced human monocytes/macrophages system.Methods: Peripheral blood mononuclear cells (PBMCs) isolated from human blood, and treated with oxidized LDL (50 μg mL-1) were used as the model system for the study. mRNA and protein expressions were studied by PCR, ELISA, and western blot analysis. ROS production and cell viability were determined by DCFDA staining and MTT assay, respectively.Results: In the presence of oxidized LDL, monocytes get differentiated into macrophages, which were confirmed by the increased expression of macrophage differentiation markers and pro-inflammatory cytokine TNF-α. Oxidized LDL increased the mRNA and protein expression of ADAMTS-4 in monocytes/macrophages. N- Acetyl cysteine, ROS scavenger, downregulate the protein expression of ADAMTS-4. The expression of ADAMTS-4 was decreased significantly in the presence of NF-κB inhibitors. SIRT-1 activity was significantly downregulated in the macrophages and was reversed in the presence of the SIRT-1 agonist, resveratrol. Acetylation of NF-κB and hence the expression of ADAMTS-4 were significantly downregulated in the presence of SIRT-1 activator, resveratrol.Conclusions: Our study suggests that oxidized LDL significantly upregulated the expression of ADAMTS-4 in the monocytes/macrophages through ROS- NF-κB- SIRT-1 pathway.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9675639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0