Physiology international最新文献

{"title":"Sesamol protects against LPS-induced inflammation in rat peritoneal macrophages by promoting SIRT1-induced repression of NF-κB.","authors":"Aparna Nandakumaran Sakunthala, Aswani Sukumaran Sreedevi, Mithra Sudha Mohan, Boban Puthenpura Thankappan, Saja Kamalamma","doi":"10.1556/2060.2024.00432","DOIUrl":"https://doi.org/10.1556/2060.2024.00432","url":null,"abstract":"<p><strong>Objectives: </strong>Sesamol, a polyphenolic compound isolated from roasted sesame seeds exhibits significant anti-inflammatory effect, but the molecular mechanism is poorly understood. Peritoneal macrophages play a pivotal role in the control of infections and inflammatory pathologies and are also found in injured tissues along with resident macrophages. The present study aimed to examine the anti-inflammatory effect of sesamol and the molecular mechanisms involved, particularly the role of sesamol in modulating SIRT1- and SIRT1-mediated deacetylation of NF-κB p65 using in vivo activated peritoneal macrophages.</p><p><strong>Materials: </strong>Sprague Dawley rats were injected with LPS to induce inflammation and sesamol was intraperitoneally administered to study its anti-inflammatory effect. ELISA and real time PCR were used to study the expression of proinflammatory cytokines. Effects of sesamol on iNOS and COX-2 were studied with activity assays and ELISA. ICAM-1, MMP-9 and TIMP-1 expressions were analysed by ELISA, RT PCR and zymography. Western blot analysis was performed to determine p65 acetylation. Nuclear translocation of p65 was evaluated by ELISA. The gene and protein expression of SIRT1 was analysed with ELISA and real time PCR.</p><p><strong>Results: </strong>Sesamol downregulated the expression of proinflammatory markers TNF-α, IL-6, iNOS, COX-2, TLR-4, ICAM-1 and MMP-9 in rat peritoneal macrophages. Additionally, sesamol upregulated SIRT1expression and attenuated the nuclear translocation of NF-κB p65 by promoting its deacetylation. Inhibition of SIRT1 by its specific inhibitor EX527 diminished the inhibitory effect of sesamol on TNF-α and IL-6. Moreover, EX527 reduced the suppressive impact of sesamol on p65 acetylation and subsequent nuclear translocation.</p><p><strong>Conclusion: </strong>Our findings suggest that the anti-inflammatory effect of sesamol involves upregulation of SIRT-1, leading to the downregulation of the nuclear translocation of NF-κB p65 through its deacetylation. Therefore, the dietary bioactive compound sesamol shows potential as a promising strategy for preventing inflammatory diseases by modulating SIRT1 expression.</p>","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Hungarian adaptation of the functional status questionnaire: Examination of validity and reliability.","authors":"István Zsenák, Alexandra Makai, Melinda Járomi","doi":"10.1556/2060.2024.00398","DOIUrl":"https://doi.org/10.1556/2060.2024.00398","url":null,"abstract":"<p><strong>Background: </strong>In the international literature, several questionnaires measuring everyday activities are known with the aim of examining patients' quality of life. In the Hungarian language, few validated questionnaires focus on daily activities, examining both basic and moderately difficult physical activity, as well as mental health.</p><p><strong>Objective: </strong>Our goal was to translate the Functional Status Questionnaire (FSQ) into Hungarian and examine its reliability and validity.The validation of the questionnaire into Hungarian followed the six-step principle formulated in 2000. In our cross-sectional study, 376 participants took part. For convergent validity, we used the 36-Item Short Form Survey (SF-36) and the Functional Independence Measure (FIM) questionnaires. The examination of internal consistency was performed through Cronbach's alpha calculations. Test-retest reliability within the class was assessed with the intraclass correlation coefficient (ICC). We conducted discriminant validity for pain and general health.</p><p><strong>Results: </strong>Internal consistency values demonstrated reliability. Participants' demographic data showed that the majority live in cities and have secondary education. The individuals included in the study exhibited unusually high levels of sports activity compared to the Hungarian average. Convergent validity examinations revealed significant relationships between dimensions of health-related quality of life of the different measurement tools.</p><p><strong>Conclusions: </strong>The validated Hungarian questionnaire is valid and reliable. Similar to the original questionnaire, it is quickly and easily completed, allowing healthcare professionals in clinical settings to assess the patient's functional status before initiating examinations.</p>","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of results of radiotherapy for oropharyngeal cancer.","authors":"Péter Trauttwein, Tibor Major, Zoltán Takácsi-Nagy","doi":"10.1556/2060.2024.00439","DOIUrl":"https://doi.org/10.1556/2060.2024.00439","url":null,"abstract":"<p><strong>Introduction: </strong>Smoking and alcohol consumption remain the two most important risk factors for the development of oropharyngeal tumours, but there is an increasing number of younger patients (age <50 years) with human papillomavirus (HPV) association origin, also known as positivity. The role of radiotherapy (RT) in the treatment of this disease is paramount.</p><p><strong>Aim: </strong>To describe the radiotherapy results for oropharyngeal tumours and to search for prognostic parameters that influence the response of these malignant lesions to radio-chemotherapy.</p><p><strong>Methods: </strong>95 patients underwent definitive radio- or radio-chemotherapy (RCT) for histologically squamous cell, oropharyngeal carcinoma at our Institute between 1 January 2019 and 31 December 2020, of which 51 (54%) received the latter. The mean age was 61.9 years (37-82 years) and the male-female ratio was 69:26. The average total dose was 69 Gy (range: 54-70 Gy).</p><p><strong>Results: </strong>The 5-year local control (LC), cancer-specific survival (CCS), and overall survival (OS) calculated by the Kaplan-Meier method were 71, 69, and 58%, respectively. Forty-four cases (46%) were confirmed to have HPV involvement. HPV positive (+) tumours showed significantly better behaviour compared to HPV negative (-) cases in LC, CCS and OS. Smoking had a significant negative effect on cure rates: LC, CCS and OS were better in non-smokers. A significant negative effect of smoking on survival was also observed in HPV-associated cases. For HPV- lesions, RCT had a stronger effect on LC than RT alone (64 vs 43%, P = 0.03).</p><p><strong>Conclusions: </strong>HPV-associated malignancies show better survival outcomes to radio ± chemotherapy than their HPV- counterparts. In all cases, smoking worsens the response to treatment. For HPV- tumours, chemotherapy with radiation, compared to irradiation alone, has a more significant effect on survival outcomes, whereas for HPV+ tumours this effect is less pronounced.</p>","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142740167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Respiratory system mechanics during noninvasive proportional assist ventilation: A model study.","authors":"Yuqing Chen, Yueyang Yuan, Hai Zhang, Feng Li, Xingwang Li","doi":"10.1556/2060.2024.00453","DOIUrl":"https://doi.org/10.1556/2060.2024.00453","url":null,"abstract":"<p><strong>Purpose: </strong>To assess the accuracies of airway resistance (Raw) and compliance (Crs) calculations using the expiratory time constant (RCexp) method as well as the accuracy of Pmus estimation in obstructive lung models.</p><p><strong>Methods: </strong>A Respironics V60 ventilator was connected to an active lung simulator. The driving pressure was maintained at 5-10 cmH2O and positive end-expiration pressure (PEEP) was 5 cmH2O. Maximal Pmus, estimated based on equations of motion and respiratory mechanical properties, was calculated by the RCexp method to derive respiratory system compliance (Crs) and inspiratory (Rinsp) and expiratory (Rexp) resistance.</p><p><strong>Results: </strong>During PAV, the assist proportion was adjusted to 55% and 40% with Pmus of 5 and 10 cmH2O, respectively. Pmus measurement errors were <20% of the preset values in most lung conditions. In the active lung model with PAV, an overestimation of Raw was found in the normal resistance condition, and Rinsp was underestimated in the severe obstructive model (P < 0.01). Crs was overestimated significantly except in the severe obstructive model at a Pmus of 10 cmH2O (all P < 0.01). Using the RCexp method, the target of ≤20% between the calculated and preset values in airway resistance was achieved in most obstructive models at a Pmus of 5 cmH2O.</p><p><strong>Conclusions: </strong>The RCexp method might provide real-time assessments of respiratory mechanics (elastance and resistance) in the PAV mode. With low inspiratory effort, the estimation error was acceptable (<20%) in most obstructive lung models.</p>","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142649072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aerobic capacity of healthy young men associated with muscle oxygen extraction rate of the vastus lateralis muscle.","authors":"Hiromichi Takeda, Kazuyuki Tabira, Wakana Yamamoto, Koume Matsuoka, Jun Horie","doi":"10.1556/2060.2024.00424","DOIUrl":"https://doi.org/10.1556/2060.2024.00424","url":null,"abstract":"<p><strong>Purpose: </strong>The determinants of aerobic capacity are oxygen delivery by the cardiopulmonary system and oxygen extraction by the skeletal muscles. However, the impact of the oxygen extraction capacity of the skeletal muscle is unclear. This study aimed to examine the associations between aerobic capacity; muscle strength, endurance, mass, and quality; and oxygen extraction capacity.</p><p><strong>Methods: </strong>Twenty-seven healthy young men (mean age, 20.7 ± 0.8 years; body mass index, 21.6 ± 3.2 kg m-2) were recruited. The following parameters were determined: peak work rate (WR) and oxygen uptake (V˙O2) corrected for body mass using the cardiopulmonary exercise testing; muscle strength and endurance using isokinetic muscle testing; muscle mass using bioelectrical impedance; muscle quality (muscle echo intensity) using an ultrasound imaging device, and muscle oxygen extraction rate (MOER) using near-infrared spectroscopy. Multiple regression analysis was performed using WR/kg peak and V˙O2/kg peak as dependent variables and each assessment index as an independent variable.</p><p><strong>Results: </strong>Multiple regression analysis with WR/kg peak as the dependent variable resulted in the adoption of SMI (β = -0.41, P = 0.036), muscle echo intensity (β = -0.45, P = 0.012) and ΔMOER (β = 0.73, P < 0.001) as significantly associated factors. Multiple regression analysis with V˙O2/kg peak as the dependent variable resulted in ΔMOER (β = 0.65, P = 0.001) being adopted as a significantly associated factor.</p><p><strong>Conclusion: </strong>These findings suggest that muscle oxygen extraction rate is associated with aerobic capacity. MOER is a useful indicator because it is not affected by body mass.</p>","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of aerobic exercise at different intensities on articular cartilage in mice.","authors":"Chunxiao He, Toshiya Tsubaki, Shota Inoue, Junpei Hatakeyama, Hanlin Jiang, Hideki Moriyama","doi":"10.1556/2060.2024.00418","DOIUrl":"10.1556/2060.2024.00418","url":null,"abstract":"<p><strong>Background: </strong>Maintaining intrinsic articular cartilage homeostasis is essential for the health of cartilage. However, the impact of aerobic exercise of varying intensities on the articular cartilage homeostasis has never been studied. This study aims to elucidate the influence of different aerobic exercise intensities on the anabolic and catabolic processes within articular cartilage.</p><p><strong>Methods: </strong>Forty-eight male C57BL/6J mice, aged 7 weeks, were divided into 4 aerobic exercise groups and 1 control group. The aerobic exercise groups were subjected to both acute and chronic exercise protocols with varying intensities of 8, 12, 16, 20, and 24 m min-1. Total RNA from the knee joint cartilage was extracted in both phases to quantify mRNA of anabolic (Sox9, Col2a1, and Acan) and catabolic (MMP-13 and ADAMTS5) markers. In the chronic exercise, articular cartilage thickness and chondrocyte density were histologically assessed. Additionally, immunohistochemical staining quantified relevant molecules involved in cartilage metabolism.</p><p><strong>Results: </strong>In the acute exercise, the 8 m min-1 group exhibited reduced ADAMTS5 expression compared to the control, 16 m min-1, and 24 m min-1 groups. Chronic exercise showed enhanced articular cartilage thickness in both the 8 and 12 m min-1 groups relative to the control group. Moreover, the 8 m min-1 group demonstrated elevated aggrecan levels in comparison to both the control and 24 m min-1 groups. Additionally, the 24 m min-1 group exhibited significantly higher ADAMTS5 levels than the control group.</p><p><strong>Conclusion: </strong>Our findings suggest that consistent low-intensity aerobic exercise suppresses catabolic molecule expression in articular cartilage, thereby fostering anabolic activity. Conversely, continuous high-intensity aerobic exercise can potentially disrupt cartilage homeostasis by enhancing catabolic processes. This dichotomy underscores the need for balanced exercise regimens to maintain cartilage health.</p>","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":" ","pages":"271-286"},"PeriodicalIF":2.2,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological characterization of a simulated kettlebell routine in experienced kettlebell athletes.","authors":"Szilárd László, Tibor Hortobágyi, Balázs Sebesi, Dávid Csala, Hargita Kató, Péter Petrovics, Artúr Krähling, Márk Váczi, Tamás Atlasz","doi":"10.1556/2060.2024.00383","DOIUrl":"10.1556/2060.2024.00383","url":null,"abstract":"<p><p>Kettlebell as a sport has gained recognition worldwide. We characterized the physiological responses induced by a simulated kettlebell competition routine in experienced kettlebell athletes (n = 26) in a two-group, pre-post plus short-term follow-up, non-randomized experiment. The experimental group (EXP) included 13 kettlebell athletes, while the control group (CON) consisted of 13 individuals with prior recreational exposure to kettlebell activities. EXP performed a 10-minute-long, long-cycle kettlebell routine, whereas CON engaged in seated rest. Cardiovascular and neuromuscular outcomes were measured at rest, after warm-up, during exercise, at 0 (immediately post), 5 and 15 min into recovery. Group-by-time interactions revealed that the 10-minute-long, long-cycle kettlebell routine increased (P < 0.05) the levels of all outcomes (e.g. heart rate, blood pressure, blood lactate) (range of effect sizes: -0.9-8.9) with many outcomes remaining well above baseline at 5 and 15 min into recovery. A notable exception was a lack of change in maximal squat strength. Kettlebell experience and mass correlated with changes in oxygen uptake (ΔVO2) and in ventilation (ΔVT) (r = -0.70, 0.64, -0.87, and 0.73, respectively, P < 0.05) in EXP. Kettlebell routine evoked significant changes in all physiological variables (respiratory and cardiovascular), out of which the heart rate (HR), diastolic blood pressure (DBP), rate pressure product (RPP), and blood lactate (BL) outlasted the routine for at least 15 min. Future studies should longitudinally examine physiological responses to kettlebell training throughout a season. Long-cycle kettlebell routine adds to the repertoire of evidence-based exercise options for high-intensity exercise.</p>","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":" ","pages":"242-257"},"PeriodicalIF":2.2,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assisted oocyte activation significantly improves zygote formation, cleavage, and implantation rates in patients with a history of fertilization failures.","authors":"Irena Antonova, Mariya Yunakova, Ivan Bochev, Nadya Magunska, Galina Yaneva, Dobri Ivanov","doi":"10.1556/2060.2024.00342","DOIUrl":"10.1556/2060.2024.00342","url":null,"abstract":"<p><strong>Background: </strong>Fertilization check performed at the 18th hour following classic in vitro fertilization procedure (IVF) or intracytoplasmic sperm injection (ICSI) is a critical stage in assisted reproduction. The success of the treatment is significantly reliant on the quantity of zygotes exhibiting two pronuclei. Consequently, low fertilization rates or complete fertilization failure are highly undesirable outcomes for both patients and reproductive specialists. Applying additional calcium ionophore for oocyte activation subsequent to ICSI may offer benefits and potentially enhance treatment outcomes, particularly for patients who have experienced low or absent fertilization rates (FR) in previous treatment cycles. The aim of the study is to evaluate the efficacy of Ca2+ ionophore application for oocyte activation.</p><p><strong>Methods: </strong>A retrospective analysis of 924 oocytes obtained from 120 patients who underwent ICSI cycles with a history of low or no fertilization as a result of previous unsuccessful treatment rounds. The next ART cycle followed with additional oocyte Ca2+ ionophore activation applied in 57 of the cases in order to optimize the treatment process (Group 1), and 63 patients were included and their outcomes followed as a control group (Group 2).We conducted a comparative analysis of results in both groups. The study's primary outcomes encompassed fertilization, cleavage embryo quality, blastocyst rate, and established clinical pregnancies.</p><p><strong>Results: </strong>At day 1 fertilization check we had 274/386 zygotes (71%FR) in group 1 and 132/410 in group 2 (32.2%FR), (P < 0.0001). Twenty-two (34.9%) cycles in group 2 resulted in total fertilization failure (TFF). At the cleavage stage top-quality embryos from group 1 were significantly higher (P = 0.0021) in comparison to group 2. Forty-eight embryo transfers (ET) were performed in group 1 resulting in 41.67% clinical pregnancies versus 33 ET and only 4 pregnancies (12.12%) for group 2 (P = 0.0044).</p><p><strong>Conclusions: </strong>The results confirm the appropriateness of assisted oocyte activation as an additional method in cases of previous fertilization failure cycles.</p>","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":" ","pages":"207-221"},"PeriodicalIF":2.2,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141992373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrophysiological detection of exam stress in health schools' students.","authors":"Hasan Kazdagli, H Fehmi Ozel, Suleyman Ozturk, Deniz Ceylan, Burak Erdeniz, Mustafa Ozbek, M Ilgi Semin","doi":"10.1556/2060.2024.00354","DOIUrl":"10.1556/2060.2024.00354","url":null,"abstract":"<p><p>Anxiety is a common issue among university students, many of them experience anxiety, depression, and stress during their school life. This study aimed to compare the acute physiological stress responses of students divided into two groups according to their perceived anxiety levels (positive test anxiety, PTA+, and negative test anxiety, PTA-). Heart rate variability (HRV) and electrodermal activity (EDA) were used to assess stress.Thirty-one healthy volunteers participated in the study. Participants completed anxiety assessments, including the Westside Test Anxiety Scale (WTAS), the State-Trait Anxiety Inventory (STAI), and the Test State Anxiety Inventory (TSAI). Based on their scores, participants were categorized into PTA+ and PTA- groups. All participants underwent 24-h continuous recordings of pulse and electrodermal activity (EDA) on two separate occasions: one day prior to a written exam and during a designated exam-free day serving as a baseline control.We compared the HRV and EDA data obtained on a regular day and on an exam day between the two groups. Results showed that the PTA+ group had significantly higher heart rate, stress index, low frequency, and short-term detrended fluctuation analysis (DFAα1) on the exam day. The tonic EDA component was also higher in the PTA+ group. Stress-related HRV and EDA parameters were negatively correlated with exam scores.In conclusion, the study found that physiological stress indicators obtained from HRV and EDA are associated with perceived exam anxiety in students.</p>","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":" ","pages":"222-241"},"PeriodicalIF":2.2,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141724159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remobilization with whole-body vibration improves functionality, histomorphometric parameters, and AQP1 expression in the soleus muscle of Wistar rats.","authors":"Maria Luiza Serradourada Wutzke, Matheus Felipe Zazula, Ana Luiza Peretti, Estéfani Marin, Jossinelma Camargo Gomes, Alberito Rodrigo de Carvalho, Célia Cristina Leme Beu, Rose Meire Costa, Lucinéia de Fátima Chasko Ribeiro, Gladson Ricardo Flor Bertolini","doi":"10.1556/2060.2024.00404","DOIUrl":"10.1556/2060.2024.00404","url":null,"abstract":"<p><strong>Background: </strong>Whole-body vibration (WBV) is used to enhance physical performance in sports and rehabilitation. The present study analyzed the effects of remobilization with WBV on the soleus muscle of Wistar rats.</p><p><strong>Methods: </strong>Twenty-eight animals were separated into four experimental groups (n = 7): CON (control); IM (immobilized); FR (immobilization and free remobilization); and WBV (immobilization and remobilization with WBV). The immobilization of the pelvic limb was carried out according to the standard protocol using a plaster cast for 15 days. For remobilization with WBV, a Frequency of 60 Hz was applied for 10 min, five days a week, for two weeks. After the remobilization period, the animals were euthanized, and the right soleus muscle was dissected followed by processing for histomorphometric analysis and immunolocalization of Aquaporin 1 (AQP1).</p><p><strong>Results: </strong>We observed a reduced larger diameter in IM compared to CON, with restored values in WBV. For the estimation of connective tissue, a significant increase was observed in the immobilized groups, while a reduction was noted in the remobilized groups. AQP1 expression decreased significantly in IM and increased in WBV.</p><p><strong>Conclusion: </strong>Immobilization caused morphofunctional damage to the soleus muscle, and remobilization with WBV is efficient and offers advantages over free remobilization.</p>","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":" ","pages":"258-270"},"PeriodicalIF":2.2,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}