Physiology international最新文献_第10页

Oral saline consumption and pressor responses to acute physical stress. 口服生理盐水的消耗和对急性生理应激的压力反应。

IF 1.4 4区医学

Physiology international Pub Date : 2020-07-16 DOI: 10.1556/2060.2020.00018

J J Del Vecchio, P A Hosick, E L Matthews

{"title":"Oral saline consumption and pressor responses to acute physical stress.","authors":"J J Del Vecchio, P A Hosick, E L Matthews","doi":"10.1556/2060.2020.00018","DOIUrl":"https://doi.org/10.1556/2060.2020.00018","url":null,"abstract":"Sodium induced volume loading may alter pressor responses to physical stress, an early symptom of cardiovascular disease.Purpose: Study 1: Determine the time point where total blood volume and serum sodium were elevated following saline consumption. Study 2: Examine the BP response to isometric handgrip (HG) and the cold pressor test (CPT) following saline consumption.Methods: Study 1: Eight participants drank 423 mL of normal saline (sodium 154 mmol/L) and had blood draws every 30 min for 3 h. Study 2: Sixteen participants underwent two randomized data collection visits; a control and experimental visit 90 min following saline consumption. Participants underwent 2 min of isometric HG, post exercise ischemia (PEI), and CPT.Results: Study 1: Total blood volume (3.8 ± 3.0 Δ%) and serum sodium (3.5 ± 3.6 Δ%) were elevated (P < 0.05) by the 90 min time point. Study 2: There were no differences in mean arterial pressure (MAP) during HG (EXP: 17.4 ± 8.2 ΔmmHg; CON: 19.1 ± 6.0 ΔmmHg), PEI (EXP: 16.9 ± 11.7 ΔmmHg; CON: 16.9 ± 7.8 ΔmmHg), or the CPT (EXP: 20.3 ± 10.8 ΔmmHg; CON: 20.9 ± 11.7 ΔmmHg) between conditions (P > 0.05). MAP recovery from the CPT was slower following saline consumption (1 min recovery: EXP; 15.7 ± 7.9 ΔmmHg, CON; 12.3 ± 8.9 ΔmmHg, P < 0.05).Conclusion: Data showed no difference in cardiovascular responses during HG or the CPT between conditions. BP recovery was delayed by saline consumption following the CPT.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":"107 2","pages":"306-318"},"PeriodicalIF":1.4,"publicationDate":"2020-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38162147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

T cell responses to an HLA-A2-restricted adipophilin peptide correlate with BMI in patients with atherosclerosis. 动脉粥样硬化患者T细胞对hla - a2限制性亲脂蛋白肽的反应与BMI相关

IF 1.4 4区医学

Physiology international Pub Date : 2020-07-13 DOI: 10.1556/2060.2020.00023

K Kalantar, Z Farzaneh, M Eshkevar Vakili, M H Karimi, M Asadi, S Khosropanah, M Doroudchi

{"title":"T cell responses to an HLA-A2-restricted adipophilin peptide correlate with BMI in patients with atherosclerosis.","authors":"K Kalantar, Z Farzaneh, M Eshkevar Vakili, M H Karimi, M Asadi, S Khosropanah, M Doroudchi","doi":"10.1556/2060.2020.00023","DOIUrl":"https://doi.org/10.1556/2060.2020.00023","url":null,"abstract":"Introduction: Atherosclerosis is an inflammatory disease causing a vast array of cardiovascular diseases. Adipophilin has been reported to be highly expressed in atherosclerotic lesions. This study investigated the possible existence of auto-reactive T cells against an HLA-A02-restricted adipophilin-derived peptide as well as peptides from Epstein-barr virus (EBV), Cytomegalovirus (CMV) and influenza (Flu) virus in patients with atherosclerosis.Methods: HLA-A02 expression on peripheral blood mononuclear cells (PBMCs) was examined by flow cytometry. PBMCs from HLA-A02 individuals were stimulated with adipophilin, CMV, EBV, and Flu peptides at a concentration of 10 µM. Interferon (IFN)-γ production was evaluated in the culture supernatant using a commercial ELISA test.Results: The levels of IFN-γ production against an HLA-A02-restricted adipophilin peptide and peptides from CMV, EBV, and Flu revealed no statistically significant differences between patients and healthy controls. However, we found a positive correlation between IFN-γ production against adipophilin and Body mass index (BMI) of patients (R = 0.8, P = 0.003), whereas no significant correlation was found in healthy controls (R = -0.267, P = 0.378). No correlation between BMI and IFN-γ production against CMV, EBV, or Flu peptides was found.Discussion: Atherosclerotic patients with higher BMIs might have greater numbers of T cells against adipophilin that is highly expressed in atherosclerotic plaques. Therefore, autoimmune reactions may have a greater role in the development of atherosclerosis in individuals with higher BMI.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":"107 2","pages":"280-293"},"PeriodicalIF":1.4,"publicationDate":"2020-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38178445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-intensity interval training-induced hypertrophy in gastrocnemius muscle via improved IGF-I/Akt/FoxO and myostatin/Smad signaling pathways in rats. 高强度间歇训练通过改善IGF-I/Akt/FoxO和肌生长抑制素/Smad信号通路诱导大鼠腓肠肌肥大

IF 1.4 4区医学

Physiology international Pub Date : 2020-07-07 DOI: 10.1556/2060.2020.00020

Soheil Biglari, Alireza Ghardashi Afousi, Farnoosh Mafi, Fatemeh Shabkhiz

{"title":"High-intensity interval training-induced hypertrophy in gastrocnemius muscle via improved IGF-I/Akt/FoxO and myostatin/Smad signaling pathways in rats.","authors":"Soheil Biglari, Alireza Ghardashi Afousi, Farnoosh Mafi, Fatemeh Shabkhiz","doi":"10.1556/2060.2020.00020","DOIUrl":"https://doi.org/10.1556/2060.2020.00020","url":null,"abstract":"Objective: It has been shown that high-intensity interval training (HIIT) leads to skeletal muscle hypertrophy; however, its mechanisms of cellular and molecular regulation are still unclear. The purpose of this study was to investigate the effect of HIIT on muscle hypertrophy and major signal transduction pathways.Design: 12 male rats were randomly divided into two groups: control and HIIT. The exercise group performed 30-min HIIT in each session (5 × 4-min intervals running at 85-95% VO2max separated by 2-min active rest at 55-60% VO2max), 3 days/week for 8 weeks. Muscle fiber cross-sectional area (CSA) and the expression of signal transduction pathway proteins were determined in the gastrocnemius muscle.Results: In the HIIT group, the expression of IGF-I, IGF-IR Akt, p-Akt, AMPKα, p-AMPKα and follistatin increased significantly, whereas a significant decrease was observed in the expression of FoxO1, p-FoxO1, myostatin, ActRIIB, Smad2/3 and p-Smad2/3 (P < 0.05). However, there were no significant differences between the HIIT and control groups in the expression of mTOR, p-mTOR, P70S6K, and p-P70S6K (P > 0.05). In addition, CSA and gastrocnemius muscle weight increased significantly in the HIIT group (P < 0.05).Conclusions: HIIT induced muscle hypertrophy by improving IGF-I/Akt/FoxO and myostatin/Smad signal transduction pathways.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":"107 2","pages":"220-230"},"PeriodicalIF":1.4,"publicationDate":"2020-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38136895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Combined effects of acute exercise and hypoxia on memory. 急性运动和缺氧对记忆的联合影响。

IF 1.4 4区医学

Physiology international Pub Date : 2020-07-02 DOI: 10.1556/2060.2020.00017

M Jung, I Brizes, S Wages, P Ponce, M Kang, P D Loprinzi

引用次数: 0

Pro- and anti-inflammatory factors, vascular stiffness and outcomes in chronic hemodialysis patients. 慢性血液透析患者的促炎因子、抗炎因子、血管僵硬度和预后。

IF 1.4 4区医学

Physiology international Pub Date : 2020-07-02 DOI: 10.1556/2060.2020.00026

B Sági, A Peti, O Lakatos, T Gyimesi, E Sulyok, I Wittmann, Botond Csiky

{"title":"Pro- and anti-inflammatory factors, vascular stiffness and outcomes in chronic hemodialysis patients.","authors":"B Sági, A Peti, O Lakatos, T Gyimesi, E Sulyok, I Wittmann, Botond Csiky","doi":"10.1556/2060.2020.00026","DOIUrl":"https://doi.org/10.1556/2060.2020.00026","url":null,"abstract":"Objective In this observational study we addressed accelerated arteriosclerosis (AS) in patients with chronic renal failure (CRF) on hemodialysis (HD) by measuring vascular stiffness (VS) parameters and attempted to relate them to pro-inflammatory and protective factors. Patients 96 consecutive patients receiving regular HD were included. 20 adult patients without major renal, cardiovascular or metabolic morbidities served as controls. Methods AS parameters (carotid-femoral pulse wave velocity - PWV, aortic augmentation index - Aix) were measured by using applanation tonometry (SphygmoCor, AtCor Medical, Sidney). In addition to routine laboratory tests 25(OH) vitamin D3 (vitamin D3) and high-sensitivity C-reactive protein (hsCRP) were quantified by immunometric assay; whereas fetuin-A, α-Klotho, tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) were determined by ELISA. Results Pro-inflammatory biomarkers (hsCRP, TNF-α and TGF-β1) were markedly elevated (P < 0.01), while anti-inflammatory factors (fetuin-A: P < 0.05, α-Klotho: P < 0.01, vitamin D3: P < 0.01) significantly depressed in HD patients when compared to controls. PWV was significantly affected only by total cholesterol, fetuin-A and dialysis time. Multiple linear regression analyses revealed that several clinical and laboratory parameters were associated with pro- and anti-inflammatory biomarkers rather than VS. The impact of baseline clinical and biochemical variables on outcome measures were also analyzed after three-year follow-up, and it was demonstrated that low levels of vitamin D, α-Klotho protein and fetuin-A were related to adverse cardiovascular outcomes, whereas all-cause mortality was associated with elevated hsCRP and depressed vitamin D. Conclusions Our results provide additional information on the pathomechanism of accelerated AS in patients with CRF, and documented direct influence of pro- and anti-inflammatory biomarkers on major outcome measures.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":"107 2","pages":"256-266"},"PeriodicalIF":1.4,"publicationDate":"2020-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38120167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Maturation of NMDA receptor-mediated spontaneous postsynaptic currents in the rat locus coeruleus neurons. 大鼠蓝斑神经元NMDA受体介导的自发突触后电流的成熟。

IF 1.4 4区医学

Physiology international Pub Date : 2020-04-23 Print Date: 2020-03-01 DOI: 10.1556/2060.2020.00010

M Kourosh-Arami, S Hajizadeh

{"title":"Maturation of NMDA receptor-mediated spontaneous postsynaptic currents in the rat locus coeruleus neurons.","authors":"M Kourosh-Arami, S Hajizadeh","doi":"10.1556/2060.2020.00010","DOIUrl":"https://doi.org/10.1556/2060.2020.00010","url":null,"abstract":"Introduction: During mammalian brain development, neural activity leads to maturation of glutamatergic innervations to locus coeruleus. In this study, fast excitatory postsynaptic currents mediated by N-methyl-d-aspartate (NMDA) receptors were evaluated to investigate the maturation of excitatory postsynaptic currents in locus coeruleus (LC) neurons.Methods: NMDA receptor-mediated synaptic currents in LC neurons were evaluated using whole-cell voltage-clamp recording during the primary postnatal weeks. This technique was used to calculate the optimum holding potential for NMDA receptor-mediated currents and the best frequency for detecting spontaneous excitatory postsynaptic currents (sEPSC).Results: The optimum holding potential for detecting NMDA receptor-mediated currents was + 40 to + 50 mV in LC neurons. The frequency, amplitude, rise time, and decay time constant of synaptic responses depended on the age of the animal and increased during postnatal maturation.Conclusion: These findings suggest that most nascent glutamatergic synapses express functional NMDA receptors in the postnatal coerulear neurons, and that the activities of the neurons in this region demonstrate an age-dependent variation.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":"107 1","pages":"18-29"},"PeriodicalIF":1.4,"publicationDate":"2020-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38101203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Left ventricular apical rotation is associated with mitral annular function in healthy subjects. Results from the three-dimensional speckle-tracking echocardiographic MAGYAR-Healthy Study. 健康受试者左心室尖顶旋转与二尖瓣环功能相关。三维斑点跟踪超声心动图magyar健康研究结果。

IF 1.4 4区医学

Physiology international Pub Date : 2020-04-23 Print Date: 2020-03-01 DOI: 10.1556/2060.2020.00002

A Nemes, Z Kovács, Á Kormányos, P Domsik, A Kalapos, Z Ajtay, C Lengyel

{"title":"Left ventricular apical rotation is associated with mitral annular function in healthy subjects. Results from the three-dimensional speckle-tracking echocardiographic MAGYAR-Healthy Study.","authors":"A Nemes, Z Kovács, Á Kormányos, P Domsik, A Kalapos, Z Ajtay, C Lengyel","doi":"10.1556/2060.2020.00002","DOIUrl":"https://doi.org/10.1556/2060.2020.00002","url":null,"abstract":"Introduction: Left ventricular (LV) twist is considered an essential part of LV function due to oppositely directed LV basal and apical rotations. Several factors could play a role in determining LV rotational mechanics in normal circumstances. This study aimed to investigate the relationship between LV rotational mechanics and mitral annular (MA) size and function in healthy subjects.Methods: The study comprised 118 healthy adult volunteers (mean age: 31.5 ± 11.8 years, 50 males). All subjects had undergone complete two-dimensional (2D) Doppler echocardiography and three-dimensional speckle-tracking echocardiography (3DSTE) at the same time by the same echocardiography equipment.Results: The normal mean LV apical and basal rotations proved to be 9.57 ± 3.33 and -3.75 ± 1.98°, respectively. LV apical rotation correlated with end-systolic MA diameter, area, perimeter, fractional area change, and fractional shortening, but did not correlate with any end-diastolic mitral annular morphologic parameters. The logistic regression model identified MA fractional area change as an independent predictor of ≤6° left ventricular apical rotation (P < 0.003).Conclusions: Correlations could be detected between apical LV rotation and end-systolic MA size and function, suggesting relationships between MA dimensions and function and LV rotational mechanics.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":"107 1","pages":"145-154"},"PeriodicalIF":1.4,"publicationDate":"2020-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38096135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The association between sympatho-vagal balance and central blood pressures. 交感迷走神经平衡与中枢血压的关系。

IF 1.4 4区医学

Physiology international Pub Date : 2020-04-23 Print Date: 2020-03-01 DOI: 10.1556/2060.2020.00005

P L Latchman, G Gates, J Pereira, R Axtell R, K Gardner, J Schlie, Q Yang, T Yue, A Morin-Viall, R DeMeersman

{"title":"The association between sympatho-vagal balance and central blood pressures.","authors":"P L Latchman, G Gates, J Pereira, R Axtell R, K Gardner, J Schlie, Q Yang, T Yue, A Morin-Viall, R DeMeersman","doi":"10.1556/2060.2020.00005","DOIUrl":"https://doi.org/10.1556/2060.2020.00005","url":null,"abstract":"Purpose: High central blood pressure is more predictive of cardiovascular disease (CVD) versus high peripheral blood pressure. Measures of central pressures (CPs) include, central systolic blood pressure (CSBP) and central diastolic blood pressure. Measures of central pressures augmentation (CPsA) include augmentation pressure (AP) and the augmentation index @ 75 beats·min-1 (AIx@75). Increased sympathetic tone (ST) is also associated with CVD. The low to high frequency ratio (LF/HF) is often used to determine sympatho-vagal balance. Given the association between ST, CPs, CPsA and CVD there is a need to understand the association between these predictors of CVD. The aims of this study were to examine the association between the LF/HF ratio, CPs, and CPsA in men and women collectively and based on gender.Methods: We measured the LF/HF ratio, CSBP, AP, and AIx@75 in 102 participants (41F/61M). The LF/HF ratio was determined via power spectral density analysis. CSBP, AP, and AIx@75 were determined via applanation tonometry.Results: The LF/HF ratio was inversely associated with AP (r = -0.26) and AIx @75 (r = -0.29) in the combined group of men and women. The LF/HF ratio was inversely associated with CSBP (r = -0.27), AP (r = -0.28), and AIx@75 (r = -0.32) in men, but not in women.Conclusion: There is an inverse association between the LF/HF ratio, AP, and AIx@75 in men and women combined. The association between the LF/HF ratio, CSBP, AP, and AIx@75, differs based on gender.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":"107 1","pages":"155-165"},"PeriodicalIF":1.4,"publicationDate":"2020-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38101201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prenatal cigarette smoke exposure slightly alters neurobehavioral development in neonatal rats: Implications for developmental origins of health and disease (DoHAD). 产前吸烟暴露轻微改变新生大鼠的神经行为发育:健康和疾病的发育起源的含义(DoHAD)。

IF 1.4 4区医学

Physiology international Pub Date : 2020-04-23 Print Date: 2020-03-01 DOI: 10.1556/2060.2020.00007

B Mammel, T Kvárik, Zs Szabó, J Gyarmati, T Ertl, J Farkas, Zs Helyes, T Atlasz, D Reglődi, P Kiss

{"title":"Prenatal cigarette smoke exposure slightly alters neurobehavioral development in neonatal rats: Implications for developmental origins of health and disease (DoHAD).","authors":"B Mammel, T Kvárik, Zs Szabó, J Gyarmati, T Ertl, J Farkas, Zs Helyes, T Atlasz, D Reglődi, P Kiss","doi":"10.1556/2060.2020.00007","DOIUrl":"https://doi.org/10.1556/2060.2020.00007","url":null,"abstract":"Numerous studies indicate that smoking during pregnancy exerts harmful effects on fetal brain development. The aim of this study was to determine the influence of maternal smoking during pregnancy on the early physical and neurobehavioral development of newborn rats. Wistar rats were subjected to whole-body smoke exposure for 2 × 40 min daily from the day of mating until day of delivery. For this treatment, a manual closed-chamber smoking system and 4 research cigarettes per occasion were used. After delivery the offspring were tested daily for somatic growth, maturation of facial characteristics and neurobehavioral development until three weeks of age. Motor coordination tests were performed at 3 and 4 weeks of age. We found that prenatal cigarette smoke exposure did not alter weight gain or motor coordination. Critical physical reflexes indicative of neurobehavioral development (eyelid reflex, ear unfolding) appeared significantly later in pups prenatally exposed to smoke as compared to the control group. Prenatal smoke exposure also resulted in a delayed appearance of reflexes indicating neural maturity, including hind limb grasping and forelimb placing reflexes. In conclusion, clinically relevant prenatal exposure to cigarette smoke results in slightly altered neurobehavioral development in rat pups. These findings suggest that chronic exposure of pregnant mothers to cigarette smoke (including passive smoking) results in persisting alterations in the developing brain, which may have long-lasting consequences supporting the concept of developmental origins of health and disease (DoHAD).","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":"107 1","pages":"55-66"},"PeriodicalIF":1.4,"publicationDate":"2020-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38101202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Exercise promotes heart regeneration in aged rats by increasing regenerative factors in myocardial tissue. 运动通过增加心肌组织中的再生因子促进老年大鼠的心脏再生。

IF 1.4 4区医学

Physiology international Pub Date : 2020-03-01 DOI: 10.1556/2060.2020.00008

A Eskandari, R Soori, S Choobineh, Z Mazaheri Tirani

{"title":"Exercise promotes heart regeneration in aged rats by increasing regenerative factors in myocardial tissue.","authors":"A Eskandari, R Soori, S Choobineh, Z Mazaheri Tirani","doi":"10.1556/2060.2020.00008","DOIUrl":"https://doi.org/10.1556/2060.2020.00008","url":null,"abstract":"Exercise-induced stem cell activation is implicated in cardiovascular regeneration. However, ageing limits the capacity of cellular and molecular remodelling of the heart. It has been shown that exercise improves structure regeneration and function in the process of ageing. Aged male Wistar rats (n = 24) were divided into three groups: Control (CO), High-intensity interval training (HIIT) (80-100% of the maximum speed), and continuous endurance training (CET) (60-70% of the maximum speed) groups. Training groups were trained for 6 weeks. The expression of the Nkx2.5 gene was determined by real-time (RT-PCRs) analysis. Immunohistochemical staining was performed to assess the C-kit positive cardiac progenitor and Ki67 positive cells. The mRNA level of Nkx2.5 was significantly increased in the CET and HIIT groups (P < 0.05). Also, cardiac progenitor cells positive for C-kit were increased in both the CET and HIIT groups (P < 0.05). Exercise training improved the ejection fraction and fractional shortening in both training groups (P < 0.05). This study indicated that training initiates the activation of cardiac progenitor cells, leading to the generation of new myocardial cells (R = 0.737, P = 0.001). It seems that C-kit positive cells in training groups showed an increase in the expression of some transcription factors (Nkx2.5 gene), representing an increased regenerative capacity of cardiomyocytes during the training period. These findings suggest that the endogenous regenerative capacity of the adult heart, mediated by cardiac stem cells, would be increased in response to exercise.","PeriodicalId":20058,"journal":{"name":"Physiology international","volume":"107 1","pages":"166-176"},"PeriodicalIF":1.4,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38007532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2