ghrelin基因rs26802变异的“GG”基因型是预防非酒精性脂肪肝的潜在保护因素。

IF 2.2 4区医学 Q3 PHYSIOLOGY

Physiology international Pub Date : 2021-09-16 DOI:10.1556/2060.2021.00183

Gholamreza Rezamand, Touraj Mahmoudi, Seidamir Pasha Tabaeian, Hamid Farahani, Fatemeh Shahinmehr, Hossein Nobakht, Reza Dabiri, Asadollah Asadi, Fariborz Mansour-Ghanaei, Mohammad Reza Zali

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引用次数: 3

摘要

背景:非酒精性脂肪性肝病(NAFLD)是一种新兴的全球性慢性肝病。考虑到NAFLD、胰岛素抵抗(IR)和肥胖之间的强大关联，以及胃饥饿素在这些代谢紊乱中的关键作用，我们假设胃饥饿素(GHRL)和胃饥饿素受体(GHSR)基因的一些单核苷酸多态性(snp)可能与NAFLD相关。方法:我们对150例活检证实的NAFLD和155例对照组进行了病例对照回顾性研究。NAFLD的诊断是在基因分型过程开始之前确定的。所有305名受试者均采用PCR-RFLP方法分型GHRL SNP rs26802或-501T>G和GHSR SNP rss572169或Arg159Arg。结果:GHRL rs26802“GG”基因型与“TT”基因型和“TT+TG”基因型相比，在校正混杂因素后仍可作为NAFLD易感性降低的标志(P = 0.006;OR = 0.14, 95% CI = 0.03-0.56, P = 0.003;OR = 0.16, 95% CI = 0.05-0.53)。然而，我们观察到GHSR SNP rs572169的基因型和等位基因频率在病例和对照组之间没有显著差异。结论:这些发现首次提示GHRL rs26802“GG”基因型对NAFLD具有保护作用。尽管如此，这一观察结果值得在其他人群中进一步调查。

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The "GG" genotype of rs26802 variant in the ghrelin gene is a potential protective factor against nonalcoholic fatty liver disease.

Background: Nonalcoholic fatty liver disease (NAFLD) is an emerging global chronic liver disease worldwide. Considering the powerful association between NAFLD, insulin resistance (IR) and obesity, as well as the key role of ghrelin in these metabolic disorders, we hypothesized that some single nucleotide polymorphisms (SNPs) of the ghrelin (GHRL) and ghrelin receptor (GHSR) genes might be associated with NAFLD.

Methods: We conducted a case-control retrospective study of 150 cases with biopsy-proven NAFLD and 155 controls. The diagnosis of NAFLD was established before the start of the genotyping process. All the 305 subjects were genotyped for GHRL SNP rs26802 or -501T>G and GHSR SNP rs572169 or Arg159Arg using the PCR-RFLP method.

Results: The GHRL rs26802 "GG" genotype compared with the "TT" genotype and "TT+TG" genotype appears to be a marker of decreased NAFLD susceptibility even after adjustment for confounding factors (P = 0.006; OR = 0.14, 95% CI = 0.03-0.56 and P = 0.003; OR = 0.16, 95% CI = 0.05-0.53, respectively). However, we observed no significant difference in genotype or allele frequencies between the cases and controls for GHSR SNP rs572169.

Conclusions: These findings proposed, for the first time, that the GHRL rs26802 "GG" genotype has a protective effect against NAFLD. Nonetheless, this observation warrants further investigations in other populations.

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来源期刊

Physiology international Medicine-Physiology (medical)

CiteScore

3.40

自引率

0.00%

发文量

期刊介绍： The journal provides a forum for important new research papers written by eminent scientists on experimental medical sciences. Papers reporting on both original work and review articles in the fields of basic and clinical physiology, pathophysiology (from the subcellular organization level up to the oranizmic one), as well as related disciplines, including history of physiological sciences, are accepted.