Pathology, research and practice最新文献_第4页

Novel drug delivery systems in colorectal cancer: Advances and future prospects 结直肠癌的新型给药系统：进展与前景

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-08-21 DOI: 10.1016/j.prp.2024.155546

引用次数: 0

Procalcitonin in inflammatory bowel disease: A diagnostic or prognostic marker 炎症性肠病中的降钙素原：诊断或预后标志物

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-08-15 DOI: 10.1016/j.prp.2024.155548

引用次数: 0

Hippo pathway effectors YAP, TAZ and TEAD are associated with EMT master regulators ZEB, Snail and with aggressive phenotype in phyllodes breast tumors Hippo通路效应因子YAP、TAZ和TEAD与EMT主调控因子ZEB和Snail有关，并与植物乳腺肿瘤的侵袭性表型有关

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-08-15 DOI: 10.1016/j.prp.2024.155551

{"title":"Hippo pathway effectors YAP, TAZ and TEAD are associated with EMT master regulators ZEB, Snail and with aggressive phenotype in phyllodes breast tumors","authors":"","doi":"10.1016/j.prp.2024.155551","DOIUrl":"10.1016/j.prp.2024.155551","url":null,"abstract":"<div><h3>Background</h3><p>Phyllodes tumors (PTs) of the breast are uncommon fibroepithelial neoplasms that tend to recur locally and may have metastatic potential. Their pathogenesis is poorly understood. Hippo signaling pathway plays an essential role in organ size control, tumor suppression, tissue regeneration and stem cell self-renewal. Hippo signaling dysfunction has been implicated in cancer. Recent evidence suggests that there is cross-talk between the Hippo signaling key proteins YAP/TAZ and the epithelial-mesenchymal transition (EMT) master regulators Snail and ZEB. In this study we aimed to investigate the expression of Hippo signaling pathway components and EMT regulators in PTs, in relation to tumor grade.</p></div><div><h3>Methods</h3><p>Expression of Hippo signaling effector proteins YAP, TAZ and their DNA binding partner TEAD was evaluated by immunohistochemistry in paraffin-embedded tissue specimens from 86 human phyllodes breast tumors (45 benign, 21 borderline, 20 malignant), in comparison with tumor grade and with the expression of EMT-related transcription factors ZEB and Snail.</p></div><div><h3>Results</h3><p>Nuclear immunopositivity for YAP, TAZ and TEAD was detected in both stromal and epithelial cells in PTs and was significantly higher in high grade tumors. Interestingly, there was a significant correlation between the expression of YAP, TAZ, TEAD and the expression of ZEB and SNAIL.</p></div><div><h3>Conclusions</h3><p>Our results originally implicate Hippo signaling pathway in PTs pathogenesis and suggest that an interaction between Hippo signaling key components and EMT regulators may promote the malignant features of PTs.</p></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141992902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA-135b mainly functions as an oncogene during tumor progression MicroRNA-135b 在肿瘤进展过程中主要作为癌基因发挥作用

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-08-15 DOI: 10.1016/j.prp.2024.155547

引用次数: 0

Expression and prognosis of DSG-2, CXADR, CD46 in head and neck squamous cell carcinoma 头颈部鳞状细胞癌中 DSG-2、CXADR 和 CD46 的表达与预后

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-08-15 DOI: 10.1016/j.prp.2024.155541

{"title":"Expression and prognosis of DSG-2, CXADR, CD46 in head and neck squamous cell carcinoma","authors":"","doi":"10.1016/j.prp.2024.155541","DOIUrl":"10.1016/j.prp.2024.155541","url":null,"abstract":"<div><h3>Objectives</h3><p>Investigating the expression and prognostic significance of adenovirus receptors DSG-2, CXADR and CD46 in head and neck cancer.</p></div><div><h3>Methods</h3><p>104 patients with HNSCC (77 OPSCC, 27 LSCC) were retrospectively included in the study. Immunohistochemical staining was performed on all selected slides to detect the expression of DSG-2, CXADR, CD46 and the immunoreactive score (IRS) was determined from the number of positively stained tumor cells and their staining intensity. Furthermore, the respective HPV status was determined by immunohistochemical staining against p16 and HPV-PCR.</p></div><div><h3>Results</h3><p>81.7 % of the tumors showed DSG-2, 34.6 % of the tumors showed CXADR and 57.7 % of the tumors showed CD46 expression. A high DSG-2 IRS correlated significantly with an advanced tumor size (p= 0.003), increased grading (p=0.012) and positive HPV status (p=0.024) in OPSCC. A high CXADR IRS was significantly associated with a positive lymph node status (p= 0.041) in LSCC and an advanced AJCC stage (p= 0.012) and a positive HPV status (p= 0.009) in OPSCC. No significant correlation could be shown regarding CD46 expression and clinical tumor data. There was no effect of DSG-2, CXADR, and CD46 expression on 5-year overall and on 5-year disease-free survival.</p></div><div><h3>Conclusion</h3><p>No prognostic significance of the expression of DSG-2, CXADR or CD46 in HNSCC was seen. DSG-2, CXADR and CD46 are expressed in HNSCC, so that optimization of oncotherapy with adenoviral vectors appears promising. Due to the significantly increased expression of DSG-2 and CXADR in advanced OPSCC tumors, there is potential for optimizing oncotherapy here in particular.</p></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0344033824004527/pdfft?md5=85ed30fd4fa364a3840447f46206d694&pid=1-s2.0-S0344033824004527-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142020695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cathepsin V is a useful prognostic factor for colorectal cancer Cathepsin V 是结直肠癌的一个有用的预后因素。

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-08-14 DOI: 10.1016/j.prp.2024.155531

{"title":"Cathepsin V is a useful prognostic factor for colorectal cancer","authors":"","doi":"10.1016/j.prp.2024.155531","DOIUrl":"10.1016/j.prp.2024.155531","url":null,"abstract":"<div><p>Molecular studies have identified various treatment-related prognostic molecules to enhance the effectiveness of colorectal cancer (CRC) treatment and improve survival rates. The expression of cathepsin V in gastrointestinal cancer cells prompted an investigation into its potential as a prognostic indicator for CRC. The evaluation of cathepsin V expression and its clinicopathological significance was conducted through immunohistochemistry in a tissue microarray, encompassing 142 CRC and normal colorectal tissues. Overall and disease-free survival rates, based on cathepsin V expression levels, were assessed using the Kaplan–Meier method and compared utilizing the log-rank test. Univariate and multivariate analyses, employing a Cox proportional hazards model, were performed to identify prognostic factors. Cathepsin V expression exhibited no correlation with age, sex, tumor location, tumor size, or histological grade. However, it was significantly correlated with depth of tumor invasion, regional lymph node (LN) metastasis, distant metastasis, and lymphovascular involvement (all p<0.001). Overall and disease-free survival rates were significantly better with low cathepsin V expression than with high expression (p<0.001). Univariate analysis identified several prognostic factors, including histological grade (low vs. high), tumor size (≤ vs. >5 cm), tumor depth (T1 vs. ≥T2), regional LN metastasis, distant metastasis, tumor-node-metastasis (TNM) stage (Stage I vs ≥II), lymphovascular involvement, and cathepsin V expression. Multivariate analysis revealed that tumor depth, distant metastasis, and cathepsin V expression are independent predictors of poor survival. Cathepsin V is frequently expressed in CRC, and its high expression is associated with poor prognosis. Therefore, cathepsin V is a useful prognostic marker for CRC.</p></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141996256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the spectrum of HER2 status in breast cancer: From HER2-positive to ultra-low HER2 了解乳腺癌中 HER2 状态的范围：从 HER2 阳性到超低 HER2

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-08-14 DOI: 10.1016/j.prp.2024.155550

{"title":"Understanding the spectrum of HER2 status in breast cancer: From HER2-positive to ultra-low HER2","authors":"","doi":"10.1016/j.prp.2024.155550","DOIUrl":"10.1016/j.prp.2024.155550","url":null,"abstract":"<div><p>HER2 (human epidermal growth factor receptor 2) status in breast cancer spans a spectrum from HER2-positive to ultra-low HER2, each category influencing prognosis and treatment decisions differently. Approximately 20 % of breast cancers overexpress HER2, correlating with aggressive disease and poorer outcomes without targeted therapy. HER2 status is determined through immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), guiding therapeutic strategies. HER2-positive breast cancer exhibits HER2 protein overexpression or gene amplification, benefiting from HER2-targeted therapies like trastuzumab and pertuzumab. In contrast, HER2-negative breast cancer lacks HER2 overexpression and amplification, treated based on hormone receptor status.</p><p>HER2-low breast cancer represents a newly recognized category with low HER2 expression, potentially benefiting from evolving therapies. Ultra-low HER2 cancers, characterized by minimal expression without gene amplification, challenge conventional classifications and treatment paradigms. Their distinct molecular profiles and clinical behaviors suggest unique therapeutic approaches. Recent diagnostic guideline updates refine HER2 assessment, enhancing precision in identifying patients for targeted therapies. Challenges remain in accurately classifying HER2-low tumors and optimizing treatment efficacy, necessitating ongoing research and innovative diagnostic methods. Understanding the heterogeneity and evolving landscape of HER2 status in breast cancer is crucial for advancing personalized treatment strategies and improving patient outcomes.</p></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142044929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging role of miR-520a in human diseases miR-520a 在人类疾病中的新作用

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-08-14 DOI: 10.1016/j.prp.2024.155545

引用次数: 0

Long non-coding RNA HOTAIR: A biomarker and therapeutic target in urological tumors 长非编码 RNA HOTAIR：泌尿系统肿瘤的生物标记物和治疗靶标

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-08-14 DOI: 10.1016/j.prp.2024.155549

引用次数: 0

Evaluation of reference genes suitable for studying mRNAs and microRNAs expression in thyroid neoplasms 评估适合研究甲状腺肿瘤中 mRNA 和 microRNA 表达的参考基因

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-08-14 DOI: 10.1016/j.prp.2024.155519

{"title":"Evaluation of reference genes suitable for studying mRNAs and microRNAs expression in thyroid neoplasms","authors":"","doi":"10.1016/j.prp.2024.155519","DOIUrl":"10.1016/j.prp.2024.155519","url":null,"abstract":"<div><p>Analysis of gene expression is a pivotal method at the core of biomarkers studies and cancer research. Currently, RT-qPCR is the most commonly used technique to investigate the expression of certain genes. The accurate and reliable result relies on an effective normalization step using suitable reference genes. The present study was designed to evaluate the eligibility of a set of candidate mRNAs and snoRNA as reference genes in the most common human thyroid neoplasms. We tested the expression levels of eleven mRNA and small RNA housekeeping genes in thyroid samples. The stability of the candidate genes was examined in different thyroid lesions and under different experimental conditions. Results were compared to the reported data in the TCGA database. Our results suggested HPRT1 and ACTB as the best mRNA reference genes, SNORD96A, and SNORD95 as the best miRNA reference genes in thyroid tissues. These genes showed the most stable expression pattern among different thyroid lesions as well as different experimental conditions. The findings in this study highlight the effect of reference genes selection on data interpretation and emphasize the importance of testing for suitable reference genes to be used in specific types of cells and experimental conditions to ensure the validity and accuracy of results.</p></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142020694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0