Pathology, research and practice最新文献

Spatial transcriptome analysis of lung squamous cell carcinoma arising from interstitial pneumonia provides insights into tumor heterogeneity.

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-12-31 DOI: 10.1016/j.prp.2024.155805

Koto Ukon, Satoshi Nojima, Daisuke Motooka, Tsuyoshi Takashima, Masaharu Kohara, Hiroki Kiyokawa, Kenji Kimura, Eriko Fukui, Shinichiro Tahara, Kansuke Kido, Takahiro Matsui, Yasushi Shintani, Daisuke Okuzaki, Eiichi Morii

{"title":"Spatial transcriptome analysis of lung squamous cell carcinoma arising from interstitial pneumonia provides insights into tumor heterogeneity.","authors":"Koto Ukon, Satoshi Nojima, Daisuke Motooka, Tsuyoshi Takashima, Masaharu Kohara, Hiroki Kiyokawa, Kenji Kimura, Eriko Fukui, Shinichiro Tahara, Kansuke Kido, Takahiro Matsui, Yasushi Shintani, Daisuke Okuzaki, Eiichi Morii","doi":"10.1016/j.prp.2024.155805","DOIUrl":"https://doi.org/10.1016/j.prp.2024.155805","url":null,"abstract":"Interstitial pneumonia (IP) is a refractory disease that causes severe inflammation and fibrosis in the interstitium of the lungs, often resulting in the development of lung cancer (LC) during treatment. Previous studies have demonstrated that the prognosis of LC complicated by IP is inferior to that of LC without IP. It is therefore of the utmost importance to gain a deeper understanding of the heterogeneity of such tumors. In the present study, we conducted spatial transcriptome analysis of squamous cell carcinoma arising from IP. The results suggested involvement of the glucocorticoid receptor pathway in treatment resistance. Immunostaining of squamous cell carcinoma specimens from patients with IP demonstrated that the tumors expressed NR3C1 to varying degrees. Furthermore, higher NR3C1 expression levels were associated with a significantly increased risk of recurrence. Our results point to a novel subtype of lung squamous cell carcinoma. Further analysis of the molecular mechanisms associated with this subtype may facilitate the development of novel diagnostic criteria and therapeutic approaches.","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"266 ","pages":"155805"},"PeriodicalIF":2.9,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relation of squamous differentiation in endometrioid carcinoma with MELF pattern to a high ratio of lymph node metastasis.

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-12-30 DOI: 10.1016/j.prp.2024.155804

Shinichiro Tahara, Kazuaki Sato, Kansuke Kido, Eiichi Morii

{"title":"Relation of squamous differentiation in endometrioid carcinoma with MELF pattern to a high ratio of lymph node metastasis.","authors":"Shinichiro Tahara, Kazuaki Sato, Kansuke Kido, Eiichi Morii","doi":"10.1016/j.prp.2024.155804","DOIUrl":"https://doi.org/10.1016/j.prp.2024.155804","url":null,"abstract":"One of the known histological patterns of endometrioid carcinoma (EC) in uterine corpus cancer is MELF (microcystic, elongated, and fragmented). MELF is associated with lymphovascular invasion and lymph node metastasis. Besides MELF, it is also known that squamous differentiation (SD) often occurs in EC. SD is known to be no significant difference in the frequency of lymph node metastasis in EC. However, there have been no previous reports on the association between MELF and SD. In this research, we investigated the presence of SD in MELF using an antibody to CK5. We examined 28 cases of EC with MELF pattern, in which 15 cases showed SD. Moreover, the relation of lymph node metastasis to SD was examined. Lymph node dissection was performed in 27 out of 28 cases. Among them, 12 cases showed lymph node metastasis. The ratio of lymph node metastasis was significantly higher in EC with SD (64.3 %, 9 in 14 cases) than EC without SD (23.1 %, 3 in 13 cases). In this study, we first showed the association between SD and MELF and that MELF with SD is associated with a high ratio of lymph node metastasis. It is clinically relevant to recognize that MELF with SD is aggressive with a high ratio of lymph node metastasis.","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"266 ","pages":"155804"},"PeriodicalIF":2.9,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Malignant transformation of the mature ovarian teratoma into early-stage ovarian adenocarcinoma: A case report with literature review.

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-12-28 DOI: 10.1016/j.prp.2024.155793

Marta Joanna Monist, Iwona Paśnik, Marek Semczuk, Andrzej Semczuk

引用次数: 0

Solid pseudopapillary neoplasm of the pancreas in a 54-year-old woman: A case report and the literature analysis.

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-12-27 DOI: 10.1016/j.prp.2024.155799

Nina Bondarenko, Alina Bilokha, Oleksii Bielosludtsev, Petro Hrytsenko, Ihor Shponka

{"title":"Solid pseudopapillary neoplasm of the pancreas in a 54-year-old woman: A case report and the literature analysis.","authors":"Nina Bondarenko, Alina Bilokha, Oleksii Bielosludtsev, Petro Hrytsenko, Ihor Shponka","doi":"10.1016/j.prp.2024.155799","DOIUrl":"https://doi.org/10.1016/j.prp.2024.155799","url":null,"abstract":"Introduction: Solid pseudopapillary neoplasm (SPN) is a rare pancreatic tumor typically occurring in young females. This case presents an instance of SPN in a 54-year-old Caucasian female, highlighting atypical age of onset and providing new insights into the tumor's clinical and histopathological diversity.Case report: A 54-year-old female with no significant past medical history presented with upper abdominal discomfort and weakness. Initial diagnostic imaging suggested a pancreatic tumor. The patient underwent laparotomic resection with pancreatic-gastric anastomosis. Histopathological analysis revealed a 2 cm tumor with mixed growth patterns - solid, trabecular, microcystic, and pseudopapillary - with varying cell types including vacuolated, eosinophilic, and clear cells. Despite showing malignant features such as local invasion in the adjacent pancreatic parenchyma, lymphovascular and perineural invasion, areas of haemorrhage, and focal nuclear atypia, no metastasis was observed. Immunohistochemistry confirmed the diagnosis of SPN with aberrant β-catenin expression. The tumor was resected successfully, and the patient had an uneventful recovery with no additional therapy required. A 60-month follow-up showed no recurrence.Conclusion: This case underscores the rarity of SPN in older patients and the variability in its histopathological presentation. Different growth patterns and microscopic malignant features of SPNs should be taken into account during histological evaluation and pathological reporting, as they may be important for determination of tumor prognostic potential and treatment strategies. Further research is needed to standardize pathologic evaluations and improve understanding of SPN recurrence and management.","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"266 ","pages":"155799"},"PeriodicalIF":2.9,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automate the process of formalin-fixed paraffin-embedded blocks storage in the pathology laboratory: A proof of concept study.

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-12-26 DOI: 10.1016/j.prp.2024.155802

Albino Eccher, Stefano Marletta, Fabio Pagni, Vincenzo L'Imperio, Federico Piacentini, Massimo Dominici, Alberto Cavazza, Carmine Pinto, Matteo Brunelli, Michelangelo Fiorentino, Umberto Malapelle, Marco Maria Baron, Guido Martignoni, Angelo Paolo Dei Tos

{"title":"Automate the process of formalin-fixed paraffin-embedded blocks storage in the pathology laboratory: A proof of concept study.","authors":"Albino Eccher, Stefano Marletta, Fabio Pagni, Vincenzo L'Imperio, Federico Piacentini, Massimo Dominici, Alberto Cavazza, Carmine Pinto, Matteo Brunelli, Michelangelo Fiorentino, Umberto Malapelle, Marco Maria Baron, Guido Martignoni, Angelo Paolo Dei Tos","doi":"10.1016/j.prp.2024.155802","DOIUrl":"https://doi.org/10.1016/j.prp.2024.155802","url":null,"abstract":"Pathology laboratories are currently facing remarkable issues in the management of their archives due to the ongoing increase in the production of formalin-fixed paraffin-embedded (FFPE) blocks, which is often coupled with inadequate spatial and environmental storing conditions. The manual process of storage and retrieving further increases the likelihood of human-based mistakes, wastes professionals' working time, and, ultimately, widens reports signing turn-around times. In the present work, we outline the strategies underlying the development of an automated archive at the pathology services of the University of Modena. The proposed project relies on the controlled interaction of a mechanic robotic arm with racks and shelves in a fully traced manner, driven by the integration with the local laboratory information system (LIS). This automated archive aims to significantly improve the time-saving of laboratory professionals and standardize the storage of FFPE samples both before and after the pathology diagnosis is rendered. The system's modularity suits the needs and spaces of the different institutions, opening novel strategies in archiving thanks to its connection with the LIS and integration with artificial intelligence algorithms.","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"266 ","pages":"155802"},"PeriodicalIF":2.9,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mast cell proteases and metastasis.

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-12-26 DOI: 10.1016/j.prp.2024.155801

Domenico Ribatti

引用次数: 0

IL-17E facilitates cell proliferation and epithelial-mesenchymal transition in A549 NSCLC cells by regulating the NF-κB pathway.

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-12-25 DOI: 10.1016/j.prp.2024.155792

Chun Li, Ying Zhao, Chengyuan He, Xingxiang Wang, Qiaotong Ren, Xiaodong Gai, Hefei Wang

{"title":"IL-17E facilitates cell proliferation and epithelial-mesenchymal transition in A549 NSCLC cells by regulating the NF-κB pathway.","authors":"Chun Li, Ying Zhao, Chengyuan He, Xingxiang Wang, Qiaotong Ren, Xiaodong Gai, Hefei Wang","doi":"10.1016/j.prp.2024.155792","DOIUrl":"https://doi.org/10.1016/j.prp.2024.155792","url":null,"abstract":"Objective: Interleukin-17 E (IL-17E) is a pro-inflammatory cytokine that participates in the inflammatory response and tumorigenesis. However, the function of IL-17E in non-small cell lung cancer (NSCLC) remains largely unknown.Methods: The clinical value of IL-17E was determined by immunohistochemistry (IHC) in 75 cases of NSCLC tissues. Furthermore, A549 cells were added with recombinant human IL-17E (rhIL-17E) or transfected with IL-17E siRNAs to evaluate the impact on cell proliferation, apoptosis, and epithelial-mesenchymal transition (EMT), as well as explore the link between IL-17E and the NF-κB pathway. Experimental techniques include CCK-8, EdU, colony formation, RT-qPCR, western blotting, flow cytometry, wound-healing, transwell and immunofluorescence assay.Results: IL-17E levels was elevated in NSCLC tissues and cells, which was related to higher TNM staging, positive lymph node metastasis and decreased tumor differentiation degree. Exogenous recombinant human IL-17E (rhIL-17E) treatment promoted cell proliferation, reduced cell apoptosis, and increased the level of Bcl-2/BAX. Moreover, it enhanced cell migration, invasion, EMT and phosphorylation levels of NF-κB p65. Inversely, knocking down endogenous IL-17E in A549 cells had the opposite effect. Blocking the NF-κB pathway with BAY-117082 reduced IL-17E expression and reversed the malignant effects induced by IL-17E on A549 cells.Conclusion: IL-17E facilitates NSCLC progression by promoting cell proliferation and EMT via the NF-κB pathway. IL-17E could serve as a potential strategy for NSCLC treatment.","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"266 ","pages":"155792"},"PeriodicalIF":2.9,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

C4d immunostaining facilitates differentiation of pemphigoid nodularis from prurigo nodularis.

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-12-25 DOI: 10.1016/j.prp.2024.155762

Fang Liu, Hui Fang, Weigang Zhang, Yuan Yuan, Zhe Yang, Lei Wang, Gang Wang

{"title":"C4d immunostaining facilitates differentiation of pemphigoid nodularis from prurigo nodularis.","authors":"Fang Liu, Hui Fang, Weigang Zhang, Yuan Yuan, Zhe Yang, Lei Wang, Gang Wang","doi":"10.1016/j.prp.2024.155762","DOIUrl":"https://doi.org/10.1016/j.prp.2024.155762","url":null,"abstract":"Pemphigoid nodularis and prurigo nodularis have similar clinicopathological features and are difficult to distinguish. Enzyme-linked immunosorbent assays (ELISA) and direct/indirect immunofluorescence can support the diagnosis of pemphigoid nodularis, but sometimes show contradictory results or are unavailable. We aimed to develop a practical method for differentiating between pemphigoid nodularis and prurigo nodularis. We analyzed the results of ELISA, direct immunofluorescence (DIF), and C3d and C4d immunohistochemistry (IHC) in 15 and 25 cases of pemphigoid nodularis and prurigo nodularis, respectively. C3d and C4d IHC results were positive in 8/15 (53.3 %) and 13/15 (86.7 %) patients with pemphigoid nodularis, respectively, and negative in all 25 patients with prurigo nodularis. The highest sensitivity (86.7 %) and negative predictive values (92.6 %) were observed in both C4d IHC and anti-BP180 ELISA, whereas C3d IHC exhibited the lowest sensitivity (53.3 %) and negative predictive values (78.1 %). Therefore, anti-BP180 ELISA and C4d IHC were the most sensitive markers to diagnose pemphigoid nodularis. The combination of DIF, ELISA, and C4d IHC is a relatively accurate panel of investigations for distinguishing pemphigoid nodularis from prurigo nodularis.","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"266 ","pages":"155762"},"PeriodicalIF":2.9,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole genome and transcriptome analysis of pancreatic acinar cell carcinoma elucidates mechanisms of homologous recombination deficiency and unravels novel relevant fusion events.

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-12-24 DOI: 10.1016/j.prp.2024.155798

Jesús Delgado-de la Mora, Majd Al Assaad, Selda Karaaslan, Kevin Hadi, Ahmed Halima, Aditya Deshpande, Jyothi Manohar, Michael Sigouros, Juan S Medina-Martínez, Michael D Lieberman, Andrea Sboner, Elizabeta C Popa, José Jessurun, Olivier Elemento, Allyson J Ocean, Erika Hissong, Juan Miguel Mosquera

{"title":"Whole genome and transcriptome analysis of pancreatic acinar cell carcinoma elucidates mechanisms of homologous recombination deficiency and unravels novel relevant fusion events.","authors":"Jesús Delgado-de la Mora, Majd Al Assaad, Selda Karaaslan, Kevin Hadi, Ahmed Halima, Aditya Deshpande, Jyothi Manohar, Michael Sigouros, Juan S Medina-Martínez, Michael D Lieberman, Andrea Sboner, Elizabeta C Popa, José Jessurun, Olivier Elemento, Allyson J Ocean, Erika Hissong, Juan Miguel Mosquera","doi":"10.1016/j.prp.2024.155798","DOIUrl":"https://doi.org/10.1016/j.prp.2024.155798","url":null,"abstract":"Pancreatic acinar cell carcinoma (PACC) is a rare pancreatic tumor with a heterogeneous clinical course and, except for radical surgery, limited treatment options. We present a comprehensive study encompassing whole-genome and RNA sequencing of 7 tumor samples from 3 metastatic PACC patients to further delineate its genomic landscape and potential therapeutic implications. Our findings reveal distinct signatures of homologous recombination deficiency (HRD) in patients harboring pathogenic germline BRCA1/2 and FANCL mutations, demonstrating favorable responses to poly (ADP-ribose) polymerase 1 (PARP) inhibitors with prolonged disease-free intervals. Additionally, we first describe structural variants in PACC, including BRCA1::TRIM47 fusion and another variant impacting FANCC, both events related to HRD, and we also identify alterations in the mitogen-activated protein kinase (MAPK) pathway, including RAF1 duplication as well as novel BRAF::SORBS2 and MAP7D2::SND1 gene fusions, offering potential targets for therapy. Our study underscores the importance of genome and transcriptome-wide profiling of PACC, to help guide personalized treatment strategies to improve patient outcomes.","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"266 ","pages":"155798"},"PeriodicalIF":2.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of TRPC1, TRPC3, and TRPC6 in the human thyroid.

IF 2.9 4区医学

Pathology, research and practice Pub Date : 2024-12-24 DOI: 10.1016/j.prp.2024.155796

Emilie Kirstein, Coline M Diebolt, Mathias Wagner, Alessandro Bozzato, Jan M Federspiel, Dirk Schaudien, Thomas Tschernig, Colya N Englisch

{"title":"Distribution of TRPC1, TRPC3, and TRPC6 in the human thyroid.","authors":"Emilie Kirstein, Coline M Diebolt, Mathias Wagner, Alessandro Bozzato, Jan M Federspiel, Dirk Schaudien, Thomas Tschernig, Colya N Englisch","doi":"10.1016/j.prp.2024.155796","DOIUrl":"https://doi.org/10.1016/j.prp.2024.155796","url":null,"abstract":"Background: Little is known about the protein expression of the transient receptor potential canonical (TRPC) channels 1, 3, and 6 in the thyroid. Research in human tissue is insufficient. Our aim was to investigate the distribution of TRPC1, 3, and 6 in the healthy human thyroid.Methods: Healthy samples were collected from seven nitrite pickling salt-ethanol-polyethylene glycol-fixed cadavers and from one patient who had undergone neck surgery (5 males, 3 females; median = 81.0, interquartile range = 6.5 years). The protein expression profiles of TRPC1, 3, and 6 were assessed using immunohistochemistry with knockout-validated antibodies. A monoclonal calcitonin antibody was used to detect calcitonin-producing C-cells.Results: All samples were labeled as healthy, displaying age-appropriate signs of degeneration. TRPC1, 3, and 6 immunolabeling in thyrocytes showed irregular staining patterns leaving selected cells with intense staining, some without. The comparison of calcitonin- and TRPC1-, 3-, and 6-immunolabeled slides strongly suggested TRPC1, 3, and 6 expression in C-cells. Connective tissue showed no immunoreactivity.Conclusions: This is, to the authors' knowledge, the first detailed description of the distribution of these channels in the human thyroid. We conclude that TRPC1, 3, and 6 are expressed in thyrocytes and C-cells of the human thyroid. Further studies are necessary to confirm these small-case-number results and to explore the relevance of these versatile channels in thyroidal health and disease.","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"266 ","pages":"155796"},"PeriodicalIF":2.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0