Pathology, research and practice最新文献

{"title":"Expression and prognostic analysis of three clones of PD-L1 antibody in colorectal cancer","authors":"Wan-Wan Gao ,&nbsp;Bing Zhou ,&nbsp;Xin Chen,&nbsp;Guo-Xiang Xu,&nbsp;Fan Zhang,&nbsp;Wei Zhang","doi":"10.1016/j.prp.2025.156144","DOIUrl":"10.1016/j.prp.2025.156144","url":null,"abstract":"<div><div>PD-L1 is a well-established marker in colorectal cancer, whose expression is a crucial indicator for the efficacy of immunotherapy. However, different PD-L1 antibodies showed different results. Our study evaluated the expressions of PD-L1 antibody from three different clones (22C3, SP263, E1L3N) in colorectal cancer tissues, we analyzed and compared their expression consistency in tumor and stroma tissues, as well as investigating the correlation between PD-L1 expression and clinicopathological parameters and patient prognosis. PD-L1 was observed in both tumor cells and immune cells, exhibiting a mottled distribution pattern. Using 1 % as the threshold, the positive rates of CPS for the three clones were 31.5 % (22C3), 28.4 % (SP263), and 32.7 % (E1L3N), respectively. When comparing 22C3 and SP263, as well as SP263 and E1L3N, a high level of consistency was observed. However, moderate consistency was found between 22C3 and E1L3N. The positive rate of CPS in different antibodies was found to be associated with mismatch repair protein (MMR) and lymphatic metastasis, while showing no correlation with gender, age, tumor size, or other parameters. Survival analyses revealed that tumor location, chemotherapy and differentiation degree emerged as independent prognostic factors for patients, whereas there is no significant correlation between PD-L1 expression and overall patient prognosis in colorectal cancer. PD-L1 expression in tumor and immune cells exhibits a variegated and mottled pattern. Three clones of PD-L1 antibody demonstrated moderate to good consistency and potential interchangeability in their expression within colorectal cancer. PD-L1 expression may serve as an indicator of tumor microenvironment in colorectal cancer patients.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"273 ","pages":"Article 156144"},"PeriodicalIF":3.2,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144723097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovarian intraoperative consultation: A 5-year study on diagnostic concordance of 657 cases","authors":"Christopher Felicelli ,&nbsp;Olivia W. Foley ,&nbsp;Steven Smith ,&nbsp;Emily Hinchcliff ,&nbsp;Luis Z. Blanco Jr","doi":"10.1016/j.prp.2025.156142","DOIUrl":"10.1016/j.prp.2025.156142","url":null,"abstract":"<div><div>Diagnostic accuracy is critical in the assessment of ovarian lesions at the time of intraoperative consult (IOC), as surgical decision making is greatly affected by the type of diagnosis rendered. In this study, we aimed to assess the diagnostic accuracy of ovarian IOC at a major academic center over a 5-year period. A total of 657 cases of ovarian lesions were included within the study. The overall accuracy of diagnosis by biological behavior was 94.2 %, and the sensitivity of IOC diagnosis was 98.7 %, 88.5 %, and 87.1 % for benign, borderline, and malignant categories, respectively. Major diagnostic errors were predominantly secondary to sampling errors, with mucinous tumors posing a challenge with frequent upgraded diagnosis. While the assessment of ovarian IOC has been assessed in the past, our study incorporates high case volumes with subsequent diversity of neoplastic lesions encountered at a major tertiary academic center. This study continues to emphasize the importance of ovarian IOC, the overall high accuracy rate, and the dependence on IOC to guide clinical colleagues.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"273 ","pages":"Article 156142"},"PeriodicalIF":2.9,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144696972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herbal management of TAA-induced liver toxicity: Fibrosis, cirrhosis, and hepatocellular carcinoma","authors":"Ahmed A. Mohamed ,&nbsp;Aly A. Shoun ,&nbsp;Rana A. El-Kadi ,&nbsp;Sandra O. Abd El-Maseh ,&nbsp;Shimaa A. Abass","doi":"10.1016/j.prp.2025.156141","DOIUrl":"10.1016/j.prp.2025.156141","url":null,"abstract":"<div><div>The liver is responsible for the metabolism of the majority of currently utilized medicines and other foreign substances. Thioacetamide (TAA) is a potent hepatotoxin organosulfur compound. Moreover, TAA gained attention in research as a tool to induce liver injuries like inflammation, fibrosis, and cirrhosis, and to study liver regeneration. Several regimens are used to prevent and treat liver disease, but they have been linked with a variety of negative effects. Several natural substances have the potential to assist in the treatment and prevention of liver disease. Multiple mechanisms contribute to the efficacy of these herbal mixtures and their bioactive compounds in preventing and treating liver disease. TAA elicited the generation of ROS, provoked oxidative stress, and initiated apoptosis in hepatic cells via the JNK and MAPK pathways. Nevertheless, the adverse effects of TAA could be suppressed by natural plants, which not only eliminated ROS but also activated the PI3K-Akt cell survival pathway in the liver and prevented apoptosis approaches. Fuller understanding of the disease etiology and fibrotic processes is important for better prevention and treatment. This review aims to describe natural plant use in preventing and treating TAA-induced liver toxicity.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"273 ","pages":"Article 156141"},"PeriodicalIF":2.9,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144696401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathological features and prognostic insights of cutaneous versus non-cutaneous angiosarcoma: A retrospective single-center cohort study","authors":"Davide Voci ,&nbsp;Alexandru Grigorean ,&nbsp;Julia Neuenschwander ,&nbsp;Franca Lisy ,&nbsp;Riccardo M. Fumagalli ,&nbsp;Tim Sebastian ,&nbsp;Nils Kucher ,&nbsp;Rolf P. Engelberger","doi":"10.1016/j.prp.2025.156139","DOIUrl":"10.1016/j.prp.2025.156139","url":null,"abstract":"<div><h3>Introduction</h3><div>Angiosarcoma is a rare, aggressive malignancy of endothelial origin, accounting for 1–2 % of soft tissue sarcomas. It occurs in cutaneous (C-AS) and non-cutaneous (NC-AS) forms, with distinct clinical behaviors and prognoses. While C-AS primarily affects the head and neck, NC-AS involves visceral organs such as the heart, liver, and breast. Despite advances in treatment, survival remains poor, and recurrence is common.</div></div><div><h3>Methods</h3><div>This retrospective, single-center cohort study analyzed 30 patients diagnosed with angiosarcoma at the University Hospital Zurich (2000–2023). The study compared clinicopathological characteristics, survival outcomes, and prognostic factors influencing survival and recurrence. Patients were stratified into C-AS (n = 10) and NC-AS (n = 20) groups. Overall survival (OS) and recurrence-free survival (RFS) were assessed using Kaplan-Meier and Cox regression analyses.</div></div><div><h3>Results</h3><div>Both subtypes shared a poor prognosis, with an overall 5-year survival rate of 12 %. NC-AS had a higher early mortality rate, with a significantly worse median OS (9 vs. 36 months, p = 0.04), while early recurrence after treatment was more frequent in C-AS. High-grade tumor differentiation and metastases at diagnosis were independent predictors of poorer OS (p = 0.01; p = 0.04). Multimodal treatment, including surgery and radiotherapy, was associated with improved survival (p &lt; 0.05). No clinicopathological factor showed a statistically significant association with RFS.</div></div><div><h3>Conclusion</h3><div>C-AS and NC-AS exhibit distinct prognostic profiles, with NC-AS associated with a higher metastatic burden and worse outcomes. Prognostic factors such as tumor grade and metastases at diagnosis influence survival. Multimodal treatment strategies appear beneficial, though recurrence remains a major challenge. Further prospective studies are needed to refine therapeutic approaches and explore emerging targeted therapies.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"273 ","pages":"Article 156139"},"PeriodicalIF":2.9,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144696971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of sildenafil combined with statins in treating pulmonary hypertension secondary to chronic obstructive pulmonary disease","authors":"Ma-Li Zhuo ,&nbsp;Jin-long Xu ,&nbsp;Wen Zhang ,&nbsp;Guo-Wu Rao ,&nbsp;Quan Zheng","doi":"10.1016/j.prp.2025.156097","DOIUrl":"10.1016/j.prp.2025.156097","url":null,"abstract":"<div><h3>Aims</h3><div>This study aims to comprehensively assess the effectiveness and safety of combining sildenafil with statins for managing pulmonary hypertension secondary to COPD. By conducting a comprehensive meta-analysis, we seek to provide robust evidence to inform and optimize clinical decision-making for this challenging condition.</div></div><div><h3>Methods</h3><div>A systematic search of the literature was performed across various databases, such as CNKI, VIP Data, Wanfang Data, PubMed, Web of Science, and the Cochrane Library, to identify RCTs that assess the effectiveness of sildenafil and statins in combination for treating PH secondary to COPD.The search included all records available from the establishment of each database until October 2024.Data extraction and meta-analysis were carried out with RevMan 5.3 software. The primary outcome measures comprised 6MWD and mPAP. The secondary outcome measures included FEV1/FVC, hs-CRP,and PaO2.</div></div><div><h3>Results</h3><div>Our study encompassed a total of 12 randomized controlled trials involving 937 patients who all come from China.Our research findings indicate that Sildenafil combined with statins could enhance 6MWD,lower mPAP,rise FEV1/FVC and PaO₂, reduce hs-CRP of COPD-PH,in contrast to the control group.Adverse reactions associated with the combination therapy were generally mild and tolerable.</div></div><div><h3>Conclusion</h3><div>This combination therapy not only improves patients' exercise capacity, as evidenced by increased 6MWD, but also alleviates PH and enhances overall quality of life. Importantly, the therapy does not raise significant safety concerns. These findings support the integration of sildenafil-statin combination therapy into the clinical management of COPD-PH, offering a promising approach to improving patient outcomes.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"273 ","pages":"Article 156097"},"PeriodicalIF":2.9,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144704604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alendronate-functionalized ZIF-8 nanoplatform for targeted delivery of SHH siRNA and docetaxel to treat prostate cancer bone metastasis","authors":"Hui Liu ,&nbsp;Wenxue Sun ,&nbsp;Jiamin Wang ,&nbsp;Longquan Xiang ,&nbsp;Xianxian Lin ,&nbsp;Rui Wang ,&nbsp;Qingbin Liu ,&nbsp;Xiangyu Zhang","doi":"10.1016/j.prp.2025.156138","DOIUrl":"10.1016/j.prp.2025.156138","url":null,"abstract":"<div><div>Sonic hedgehog (SHH) signaling plays a crucial role in prostate carcinogenesis, especially in the context of bone metastasis. SHH can activate the SHH signaling network in the bone metastasis microenvironment by promoting osteoclast maturation and osteoblast calcium deposition, both of which accelerate bone metastasis progression. We here designed a multifunctional ZIF-8 lipid nanoparticle drug delivery system to enhance therapeutic effectiveness. The system was loaded with SHH siRNA at the core and docetaxel (DTXL) in the outer layers of the nanoparticle (NP). The NP surface was modified with the alendronate-conjugated DOPE-PEG polymer to specifically target bone metastasis. The SHH siRNA and DTXL-loaded NPs effectively inhibited the <em>in vitro</em> proliferation and colony formation of PC-3 cells, induced their G2/M phase cell cycle arrest, and promoted apoptosis. Moreover, the conditioned medium from the PC-3 and MC3T3-E1 co-culture triggered osteoclast and osteoblast differentiation. In the mouse model of prostate cancer bone metastasis, these ZIF-8 NPs selectively accumulated at the bone metastasis sites, effectively suppressing prostate cancer growth. Additionally, these drug-loaded ZIF-8 NPs exhibited good biocompatibility, making them a promising therapeutic approach for prostate cancer bone metastasis.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"273 ","pages":"Article 156138"},"PeriodicalIF":2.9,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and pathological analysis of 17 cases of mesonephric-like adenocarcinoma","authors":"Biqian Kang ,&nbsp;Bo Han ,&nbsp;Danhua Shen ,&nbsp;Guo Zhang ,&nbsp;Xiaobo Zhang","doi":"10.1016/j.prp.2025.156110","DOIUrl":"10.1016/j.prp.2025.156110","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Mesonephric-like adenocarcinoma (MLA) is a rare gynecological malignancy，during diagnosis, it is frequently mistaken for other gynecological malignancies because different development patterns coexist under a microscope. This study aims to provide a more comprehensive understanding of the disease by analyzing the clinical and pathological characteristics, immunohistochemistry, and molecular features of MLA.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;A retrospective analysis was conducted on 17 patients with mesonephric-like adenocarcinomadiagnosed by the Department of Pathology at Peking University People's Hospital from January 2021 to June 2025. The lesions were subjected to HE staining and immunohistochemical staining, and 10 cases were examined for gene mutations by next generation sequencing. The results of HE staining, immunohistochemical staining, and molecular testing were examined, along with the clinical information and histological characteristics.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;The age of the patients ranged from 49 to 83 years (median age 62.3 years), with 3 cases occurred in the ovaries and 14 cases in the uterus. 7 patients presented with (postmenopausal) irregular vaginal bleeding, 3 patient was diagnosed with ovarian or endometrial mass detected by ultrasound during a physical examination, 1 patient was diagnosed with complex endometrial hyperplasia during a hysteroscopy, 1 patient was diagnosed after further examination due to an elevated CA199 level, and 1 patient presented with pain from a liver metastasis. The initial symptoms of the remaining 4 cases referred from other hospitals were unknown. The tumor sizes ranged from 1.3 × 0.8 × 0.5 cm to 8.3 × 7.1 × 4 cm, with solid or cystic-solid growth patterns. Immunohistochemistry showed that 12 cases had negative expression of the estrogen receptor (ER) and progesterone receptor (PR). 13 cases showed an inverse expression relationship between TTF-1 and GATA-3. 12 cases showed patchy positive expression for P16, 10 cases had CD10 positive expression at the luminal edge, and 15 cases had P53 expression that was wild-type. 9 of the 10 patients who had genetic testing had mutations in the KRAS gene at codon 12, 3 had mutations in the PTEN gene, 2 had mutations in the CTNNB1, 1 had a TP53 mutation, 1 had a PIK3CA mutation, 1 had a CCND1 mutation, 1 had a CDK6 mutation, 1 had a BCL2L11 mutation, and 1 had a non-specific molecular profile (NSMP).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;Mesonephric-like adenocarcinoma is a rare type of gynecologic malignant tumor. Because of its complex histomorphology, pathologists have to distinguish it from other gynecological tumors during diagnosis. The expression of TTF-1, GATA-3, and CD10 in immunohistochemistry can assist the diagnosis. Apart from immunohistochemical indicators, MLA is frequently linked to gene mutations like KRAS, TP53, and CTNNB1, which can aid pathologists in their diagnosis. MLA can spread to dista","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"273 ","pages":"Article 156110"},"PeriodicalIF":3.2,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nucleus pulposus cell-associated PLAU promotes intervertebral disc degeneration through HIPPO pathway.","authors":"Yan An ,&nbsp;Xiang Zhu ,&nbsp;Xingye Li ,&nbsp;Lin Zhang ,&nbsp;Muyu Gu ,&nbsp;Yajun Liu","doi":"10.1016/j.prp.2025.156137","DOIUrl":"10.1016/j.prp.2025.156137","url":null,"abstract":"<div><div>Intervertebral disc degeneration (IDD) is the most common and critical pathological basis for various spinal disorders. In this study, we investigated how plasminogen activator urokinase (PLAU) promotes IDD progression through the HIPPO pathway. Bioinformatics analysis showed that PLAU is a hub gene in IDD. Both in vitro and in vivo experiments confirmed that PLAU can promote the apoptosis of nucleus pulposus chondrocytes. PLAU activates the HIPPO signaling pathway by increasing the phosphorylation levels of mammalian ste20-like kinases 1/2 (MST1/2), large tumor suppressor 1/2 (LATS1/2), and yes associated protein (YAP), promoting chondrocyte apoptosis, which is one of the reasons for the accelerated progression of IDD. These findings provide new insights into the role of PLAU in IDD and highlight its potential as a therapeutic target for IDD.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"273 ","pages":"Article 156137"},"PeriodicalIF":2.9,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The unexplored Nexus: Mitochondria derived microproteins and Parkinson’s disease","authors":"Kashish Patel,&nbsp;Ritu Soni,&nbsp;Jigna Shah","doi":"10.1016/j.prp.2025.156136","DOIUrl":"10.1016/j.prp.2025.156136","url":null,"abstract":"<div><div>Parkinson's disease (PD) is the second most common neurodegenerative disorder and mainly occurs in people above the age of 60 years. It is defined by the progressive degeneration of dopaminergic neurons of the substantia nigra pars compacta, which results in the classic motor symptoms. Though aggregation of alpha-synuclein and Lewy body formation are still the core of the disease pathogenesis, PD pathogenesis is complex with mitochondrial dysfunction, oxidative stress, neuroinflammation, impaired autophagy, and endoplasmic reticulum (ER)-Golgi stress. Of these, mitochondrial dysfunction has been the focus of special interest because of its key function in energy metabolism and generation of reactive oxygen species (ROS), which can hasten the neuronal damage. Over the past few years, mitochondrial-derived peptides (MDPs), also k/a mitochondrial microproteins such as Humanin, Small Humanin-Like Peptides (SHLPs), and Mitochondrial Open Reading Frame of the 12S rRNA type-c (MOTS-c) have been identified as new hope for modulating cellular stress responses. Their therapeutic opportunities to impact major pathogenic processes in PD, including inflammation, oxidative stress, and metabolic dysfunction, make them new targets for disease-modifying therapies. With the escalating load of PD and the limitation of existing symptomatic therapies, novel molecular targets need to be explored urgently. Research into the mechanisms involving MDPs in PD not only enhances the insight into disease mechanisms but could potentially lead the way toward next-generation therapies. This article is intended to thoroughly review the role of MDPs in PD pathogenesis and highlight their importance as novel therapeutic agents. With the growing burden of PD and the absence of disease-modifying therapies, exploring novel mitochondrial signaling pathways offers an urgently needed therapeutic direction.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"273 ","pages":"Article 156136"},"PeriodicalIF":2.9,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the enigmatic role of MTBP in pan-cancer: A bioinformatics perspective","authors":"Weiqun Li ,&nbsp;Fangping Wu ,&nbsp;Xinjie Wu ,&nbsp;Liang Chen ,&nbsp;Han Wang ,&nbsp;Jianbo Yu","doi":"10.1016/j.prp.2025.156121","DOIUrl":"10.1016/j.prp.2025.156121","url":null,"abstract":"<div><h3>Background</h3><div>The role of MDM Two Binding Protein (MTBP) in tumor regulation is controversial;</div></div><div><h3>Methods</h3><div>We gathered MTBP's genomic location and GTEx expression data from University of California Santa Cruz (UCSC) and utilized the Human Protein Atlas (HPA) to evaluate its expression. Survival prognosis and immune infiltration analysis were meticulously evaluated by downloading and processing TCGA RNAseq data. Genetic alterations in MTBP were explored using cBioPortal; (3) Our findings revealed a prevailing trend of MTBP overexpression in numerous cancer types, associating with adverse prognoses and implicating MTBP as an oncogenic facilitator. Notably, in certain cancers, reduced MTBP expression correlated with improved patient survival, reflecting a tumor suppressive function of MTBP. By analyzing the tumor immunological environment, we observed positive correlations between MTBP and specific T helper cell subsets, suggesting its potential role in immune evasion strategies. Phosphorylation emerged as a pivotal post-translational modification regulating MTBP’s function, influencing DNA replication process frequently exploited by cancer cells. Functional enrichment analyses highlighted MTBP's involvement in nuclear division, organelle fission, and cell cycle regulation, reinforcing its centrality in tumor proliferation and differentiation;</div></div><div><h3>Conclusions</h3><div>Our results position MTBP as a critical regulator of cancer progression, with implications for immune modulation and DNA replication control.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"273 ","pages":"Article 156121"},"PeriodicalIF":2.9,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}