LINC00673 promotes osteosarcoma progression through the miR-92b-3p/DUSP1 axis

IF 3.2 4区医学 Q2 PATHOLOGY

Pathology, research and practice Pub Date : 2025-09-29 DOI:10.1016/j.prp.2025.156253

Kailiang Zhang , Mingrui Du , Ming Chen , Yinglong Zhang , Kuohao Shi , Dawei Zhang , Xin Wang , Yong Zhou

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引用次数: 0

Abstract

Background

An increase body of research indicates that long non-coding RNAs (lncRNAs) play a critical role in the development of osteosarcoma. This study investigates the function and molecular mechanism of LINC00673 in the progression of osteosarcoma.

Methods

Quantitative reverse transcription-PCR (qRT-PCR) was employed to assess the expression levels of LINC00673 in osteosarcoma cells and tissues. Additionally, we evaluated the correlation between LINC00673 expression in osteosarcoma tissues and the clinicopathological characteristics of patients. Various assays, including CCK-8, Colony formation assay, Transwell assay, and nude animal model experiments, were conducted to investigate the biological function of LINC00673 in osteosarcoma both in vivo and vitro. Downstream target genes of LINC00673 were identified through whole transcriptome sequencing and bioinformatics tools, followed by validation using qRT-PCR, western blotting, RNA immunoprecipitation (RIP) assay, dual-luciferase reporter gene assay and rescue experiments.

Results

In our study, we found that LINC00673 is highly expressed in osteosarcoma cells and tissues. The upregulation of LINC00673 was positively correlated with advanced clinical stages and distant metastasis in patients with osteosarcoma. The knockdown of LINC00673 suppressed both cell proliferation and metastasis of osteosarcoma in vivo and vitro. Subsequent mechanistic studies revealed that LINC00673 functions as a competing endogenous RNA (ceRNA). enhancing DUSP1 expression by sponging miR-92b-3p.

Conclusions

LINC00673 functions as an oncogenic lncRNA in osteosarcoma by enhancing the malignant phenotype of osteosarcoma through miR-92b-3p/DUSP1 axis.

查看原文本刊更多论文

LINC00673通过miR-92b-3p/DUSP1轴促进骨肉瘤进展。

背景：越来越多的研究表明，长链非编码rna （lncRNAs）在骨肉瘤的发生发展中起着关键作用。本研究探讨LINC00673在骨肉瘤发生发展中的作用及其分子机制。方法：采用定量逆转录pcr （qRT-PCR）技术检测骨肉瘤细胞和组织中LINC00673的表达水平。此外，我们评估了骨肉瘤组织中LINC00673表达与患者临床病理特征之间的相关性。通过CCK-8、菌落形成实验、Transwell实验、裸体动物模型实验等多种实验，研究LINC00673在骨肉瘤中的体内外生物学功能。通过全转录组测序和生物信息学工具对LINC00673下游靶基因进行鉴定，随后采用qRT-PCR、western blotting、RNA免疫沉淀（RIP）实验、双荧光素酶报告基因实验和拯救实验进行验证。结果：在我们的研究中，我们发现LINC00673在骨肉瘤细胞和组织中高表达。LINC00673的上调与骨肉瘤患者的晚期临床分期和远处转移呈正相关。在体内和体外实验中，敲低LINC00673对骨肉瘤细胞增殖和转移均有抑制作用。随后的机制研究表明，LINC00673具有竞争性内源性RNA （ceRNA）的功能。通过海绵miR-92b-3p增强DUSP1的表达。结论：LINC00673在骨肉瘤中作为一种致癌lncRNA，通过miR-92b-3p/DUSP1轴增强骨肉瘤的恶性表型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pathology, research and practice 医学-病理学

CiteScore

5.00

自引率

3.60%

发文量

405

审稿时长

24 days

期刊介绍： Pathology, Research and Practice provides accessible coverage of the most recent developments across the entire field of pathology: Reviews focus on recent progress in pathology, while Comments look at interesting current problems and at hypotheses for future developments in pathology. Original Papers present novel findings on all aspects of general, anatomic and molecular pathology. Rapid Communications inform readers on preliminary findings that may be relevant for further studies and need to be communicated quickly. Teaching Cases look at new aspects or special diagnostic problems of diseases and at case reports relevant for the pathologist''s practice.