Pathology, research and practice最新文献

{"title":"Influencing factors on detection rate of granuloma in Crohn's disease: A retrospective analysis","authors":"Yinxian Shen ,&nbsp;Jun Wang ,&nbsp;Haoying Liu ,&nbsp;Shangzhan Huang ,&nbsp;TingTing Qin ,&nbsp;Xuantao Ji ,&nbsp;Jiayuan Huang ,&nbsp;Qi Zhou ,&nbsp;Jiazhi Liao ,&nbsp;Fang Xiao","doi":"10.1016/j.prp.2025.156054","DOIUrl":"10.1016/j.prp.2025.156054","url":null,"abstract":"<div><h3>Background</h3><div>The detection of non-caseating granulomas in histopathological analysis is a key criterion for Crohn’s disease (CD) diagnosis. The low frequency of granuloma identification poses a significant challenge to early and accurate diagnosis. This study aims to identify factors affecting granuloma detection rate in CD patients, as well as assess how endoscopic severity and lesion types influence detection, ultimately improving biopsy accuracy and non-caseating granuloma detection rate.</div></div><div><h3>Methods</h3><div>A retrospective analysis of 308 granuloma-positive CD patients was performed. Age, sex, Montreal classification, endoscopic findings, modified SES-CD scores, and histopathology were collected. Associations between granuloma detection (positive vs. negative) and bowel segment, specimen quantity, and size were evaluated using Chi-square tests. Endoscopic images were graded by modified SES-CD criteria.</div></div><div><h3>Results</h3><div>Granuloma detection was significantly higher in specimens ≥ 0.1 cm compared to &lt; 0.1 cm (p &lt; 0.001) and increased with specimen quantity, reaching 68.13 % at N ≥ 5 (p &lt; 0.001). The terminal ileum, left colon, and right colon had significantly higher detection rates than the rectum (p &lt; 0.005). Detection rates were higher in the endoscopic mild-to-moderate group than the severe group (p &lt; 0.016) and the normal group (p = 0.001). Ulcerative lesions had significantly higher detection than non-ulcerative lesions (p = 0.001).</div></div><div><h3>Conclusions</h3><div>The granuloma detection rate correlates with bowel segment, specimen size and quantity, lesion severity, and morphology. Biopsying from regions of mild-to-moderate lesions may enhance granuloma detection.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"272 ","pages":"Article 156054"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combating oxidative stress in non-alcoholic fatty liver disease: From mechanisms to therapeutic strategies","authors":"Amr Ali Mohamed Abdelgawwad El-Sehrawy ,&nbsp;Abdulsalam Najm Mohammed ,&nbsp;Jitendra Gupta ,&nbsp;Jaafaru Sani Mohammed ,&nbsp;R. Roopashree ,&nbsp;Aditya Kashyap ,&nbsp;Bethanney Janney J ,&nbsp;Samir Sahoo ,&nbsp;Shaker Al-Hasnaawei ,&nbsp;Yassir Mohammed Nasr","doi":"10.1016/j.prp.2025.156053","DOIUrl":"10.1016/j.prp.2025.156053","url":null,"abstract":"<div><div>Non-alcoholic fatty liver disease (NAFLD) is a prevalent liver disorder with a growing global impact, closely linked to metabolic syndrome and lifestyle factors. Oxidative stress, arising from an imbalance between reactive oxygen species (ROS) production and antioxidant defenses, plays a pivotal role in the pathogenesis of NAFLD. It drives lipid peroxidation, mitochondrial dysfunction, and inflammatory cascades, exacerbating hepatic steatosis and promoting progression to non-alcoholic steatohepatitis (NASH), fibrosis, and cirrhosis. This review delves into the molecular mechanisms of oxidative stress in NAFLD, highlighting its contribution to disease initiation and progression. It explores key sources of ROS, such as mitochondrial dysfunction, and discusses the interplay between oxidative stress, insulin resistance, and inflammation. Strategies to mitigate oxidative damage, including lifestyle modifications, dietary antioxidants, and emerging pharmacological interventions, are examined with a focus on their therapeutic potential. By unraveling the oxidative stress pathways in NAFLD, this review aims to provide insights into effective preventive measures and therapeutic approaches. Understanding these mechanisms offers a foundation for future research and clinical innovations to combat the burden of NAFLD and its complications.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"272 ","pages":"Article 156053"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice to “MicroRNA-129–5p suppresses proliferation, migration and invasion of retinoblastoma cells through PI3K/AKT signaling pathway by targeting PAX6” [Pathol. - Res. Pract. 215 (2019) 152641]","authors":"Yang Liu ,&nbsp;Guodong Liang ,&nbsp;Hong Wang ,&nbsp;Zengshan Liu","doi":"10.1016/j.prp.2025.156029","DOIUrl":"10.1016/j.prp.2025.156029","url":null,"abstract":"","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"271 ","pages":"Article 156029"},"PeriodicalIF":2.9,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ensemble learning approach for detecting breast invasive ductal carcinoma from histopathological images","authors":"Himanish Shekhar Das,&nbsp;Kasmika Borah,&nbsp;Kangkana Bora","doi":"10.1016/j.prp.2025.156041","DOIUrl":"10.1016/j.prp.2025.156041","url":null,"abstract":"<div><div>Invasive ductal carcinoma is a type of breast cancer that is one of the most frequent and aggressive forms of breast malignancy, necessitating accurate and timely diagnosis for effective treatment. Though considered the gold standard, traditional histopathological diagnosis is subject to inter-observer and intra-observer variability, potentially impacting patient outcomes. This study proposed an ensemble learning approach for classifying invasive ductal carcinoma to address these challenges. The proposed method combines the strengths of multiple deep-learning models to enhance diagnostic accuracy and robustness. We employed a diverse set of pre-trained convolutional neural networks, viz, ResNet50, Xception, MobileNetV2, VGG16, and VGG19, each trained on histopathological images of breast histology slides. These five different deep learning models were compared in this work, and the resulting inference results are also shown. Ensemble and a fine-tuning approach to transfer learning were also used to extract the best results. These models were evaluated using evaluation metrics like accuracy to see which one does the job best. The proposed weighted average ensemble algorithm achieved 97.27 % accuracy. Among all models, the ResNet50 model outperforms the other models in identifying invasive ductal carcinoma. Therefore, ResNet50 is the preferred model when accuracy is the top concern for a particular resolution image, and the weighted average ensemble approach enhances the performance of the proposed work. Our results indicate that the proposed ensemble approach decreases variability in diagnoses and advancements in accuracy. This method holds promise for enhancing the precision of breast cancer diagnostics, potentially leading to better patient management and outcomes.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"272 ","pages":"Article 156041"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ALYREF regulates the m5C modification and stability of BIRC5 mRNA to promote ovarian cancer progression","authors":"Yongju Tian ,&nbsp;Jianmeng Liu ,&nbsp;Lei Sun ,&nbsp;Xiaoguang Wang","doi":"10.1016/j.prp.2025.156055","DOIUrl":"10.1016/j.prp.2025.156055","url":null,"abstract":"<div><h3>Background</h3><div>Ovarian cancer (OC) is the most dangerous cancer among gynecological tumors, which poses a significant threat to women’s health worldwide. 5-methylcytosine (m5C) plays a significant role in regulating tumor development. The RNA methyltransferase Aly/REF export factor (ALYREF) is one of the m5C-modified RNA-reading proteins. Although it was reported that ALREF played a facilitating role in a variety of cancers, the mechanism of ALYREF in OC was unclear.</div></div><div><h3>Methods</h3><div>Bioinformatics and western blot were applied to analyze the expression levels of ALYREF both in tissues and cells of OC. Cellular behaviors were detected by CCK8, colony formation assay, EdU assay and flow cytometry. Then, glucose and lactate levels were measured to assess glycolysis. Next, RIP, MeRIP and qRT-PCR were used to explore the interaction between ALYREF and baculoviral IAP repeat containing 5 (BIRC5). At last, the roles of ALYREF and BIRC5 <em>in vivo</em> were proved by constructing xenograft tumor models.</div></div><div><h3>Results</h3><div>ALYREF was highly expressed both in tissues and cells of OC. Downregulated ALYREF alleviated cell proliferation, migration, invasion, cell cycle, and glycolysis, and promoted apoptosis. Moreover, ALYREF had binding sites with BIRC5, and their expressions in tumors were positively correlated. The anti-tumor effects due to the downregulation of ALYREF could be reverted by overexpression of BIRC5. Furthermore, ALYREF knockdown restrained the growth of OC <em>in vivo</em>.</div></div><div><h3>Conclusion</h3><div>ALYREF regulates BIRC5 mRNA by m5C modification and exerts oncogenic effects in OC.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"272 ","pages":"Article 156055"},"PeriodicalIF":2.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical features and therapeutic experiences of renal cancer related to Xp11.2 translocation/TFE3 gene fusion: A case series of 18 patients","authors":"Zehua Shu ,&nbsp;Yang Lv ,&nbsp;Mimi Zhao ,&nbsp;Xinyi Liu ,&nbsp;Qiang Ma ,&nbsp;Siming Fu ,&nbsp;Gaolei Liu ,&nbsp;Weihua Lan ,&nbsp;Yao Zhang","doi":"10.1016/j.prp.2025.156052","DOIUrl":"10.1016/j.prp.2025.156052","url":null,"abstract":"<div><h3>Background</h3><div>This study aims to summarize the clinical features, imaging characteristics, and treatment outcomes of Xp11.2 translocation/TFE3 gene fusion-associated renal cell carcinoma (Xp11.2 tRCC), a rare and distinct subtype of kidney cancer.</div></div><div><h3>Methods</h3><div>A retrospective review was conducted on 18 patients diagnosed with Xp11.2 tRCC. Clinical presentations, imaging findings, treatment modalities, and follow-up data were systematically analyzed.</div></div><div><h3>Results</h3><div>Among the 18 patients, 10 had tumors in the left kidney and 8 in the right. Tumor sizes on CT ranged from 2.5 to 12.5 cm. Plain CT scan showed that 8 cases of tumors were round, 6 cases were mass-like, and 4 cases had irregular shapes. Fifteen tumors presented as solid masses, while 3 were cystic. On T1-weighted imaging (T1WI), 8 tumors showed iso- or slightly hypointense signals, and 10 were hyperintense. T2-weighted imaging (T2WI) revealed heterogeneous signal intensity in 12 tumors, while 3 appeared hypointense. Surgical resection was the primary treatment: 8 patients underwent radical nephrectomy and 9 underwent partial nephrectomy. One patient with metastatic disease at diagnosis received targeted therapy. Seventeen patients were followed up (median: 35 months); one was lost to follow-up. During the follow-up period, two patients developed metastatic disease. One experienced metastases to the liver and lumbar vertebrae, while the other presented with widespread systemic metastases. Both patients were referred to the oncology department of our institution for specialized treatment.</div></div><div><h3>Conclusion</h3><div>Xp11.2 tRCC is a rare subtype of renal cell carcinoma. The imaging findings associated with this condition possess distinct characteristics that can enhance the accuracy of preoperative diagnosis.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"271 ","pages":"Article 156052"},"PeriodicalIF":2.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cisplatin potentiates PD-L1 expression more robustly than pemetrexed in malignant pleural mesothelioma: Temporal dynamics revealed by cellular and xenograft analyses","authors":"Zhenghua Zhang ,&nbsp;Wenjun Gao ,&nbsp;Feng Yuan ,&nbsp;Yubin Hu ,&nbsp;Xiaoyu Tuo ,&nbsp;Liangping Luo ,&nbsp;Xiaonan Tang ,&nbsp;Shasha Shen ,&nbsp;Yang Tian ,&nbsp;Dan Han","doi":"10.1016/j.prp.2025.156048","DOIUrl":"10.1016/j.prp.2025.156048","url":null,"abstract":"<div><h3>Objective</h3><div>Chemotherapy may modulate PD-L1 expression in malignant pleural mesothelioma (MPM), influencing immune checkpoint inhibitor (ICI) efficacy. We compared cisplatin (CDDP) and pemetrexed (PEM) on PD-L1 dynamics in MPM.</div></div><div><h3>Methods</h3><div>H226 (epithelial) and MSTO-211H (biphasic) cells were treated with CDDP/PEM for 12–48 h, with apoptosis analyzed by flow cytometry and PD-L1 by Western blot. Xenograft models (CDDP/PEM-treated mice) assessed tumor growth and PD-L1 via immunohistochemistry.</div></div><div><h3>Results</h3><div>Both drugs inhibited cell growth and induced apoptosis (CDDP &gt; PEM, <em>P</em> &lt; 0.05). Baseline PD-L1 was higher in MSTO-211H vs. H226 (<em>P</em> &lt; 0.05). Chemotherapy upregulated PD-L1, peaking at 48 h (CDDP &gt; PEM, <em>P</em> &lt; 0.05). In xenografts, MSTO-211H tumors showed faster growth and higher PD-L1 (<em>P</em> &lt; 0.05), further amplified by CDDP during progression.</div></div><div><h3>Conclusion</h3><div>CDDP robustly potentiates PD-L1 in MPM, especially in biphasic subtypes. Temporal PD-L1 dynamics suggest chemotherapy-ICI synergy may depend on drug selection and treatment timing.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"271 ","pages":"Article 156048"},"PeriodicalIF":2.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144154543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromatin architecture protein HMGA2 promotes glioma malignancy via novel mechanism of IGFBP3 transcription inhibition","authors":"Zenghua Liang ,&nbsp;Lu Qiao ,&nbsp;Pengyi Ma ,&nbsp;Shanshan Zhang ,&nbsp;Cuiyun Sun ,&nbsp;Wenjun Luo ,&nbsp;Lin Yu","doi":"10.1016/j.prp.2025.156051","DOIUrl":"10.1016/j.prp.2025.156051","url":null,"abstract":"<div><h3>Background</h3><div>Glioma, the most prevalent primary brain tumor, is characterized by rapid proliferation, invasive growth patterns, and poor clinical outcomes. This study investigates the expression and clinical significance of chromatin architecture protein high mobility group AT-hook 2 (HMGA2) in glioma, aiming to identify potential prognostic biomarkers and therapeutic targets.</div></div><div><h3>Methods</h3><div>The expression of HMGA2 in different grades glioma samples was analyzed by immunohistochemistry (IHC). The functions of HMGA2 in glioma cells were identified by migration, invasion, proliferation and orthotopic tumor transplantation assays. The downstream genes of HMGA2 were screened by RNA-Seq. Chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR), electrophoretic mobility shift assay (EMSA) and chromosome conformation capture assay (3 C) were used to analyze the downstream mechanism of HMGA2 in glioma cells.</div></div><div><h3>Results</h3><div>We demonstrated a positive correlation between HMGA2 expression levels and glioma malignancy grade through IHC analysis. Multivariate COX regression analysis further established HMGA2 as an independent prognostic factor in glioma. Our functional studies revealed that HMGA2 significantly enhances the migration, invasion, and proliferation capabilities of glioblastoma (GBM) cells. Mechanistically, we identified insulin-like growth factor binding protein 3 (IGFBP3) as a novel downstream target of HMGA2. HMGA2 mediates transcriptional repression of IGFBP3 through disruption of promoter-enhancer interactions, leading to subsequent activation of the PI3K/Akt signaling pathway and promotion of malignant phenotypes in GBM.</div></div><div><h3>Conclusion</h3><div>We confirmed a novel chromatin conformation-mediated transcriptional repression mechanism of HMGA2. By regulating IGFBP3 expression and modulating the PI3K/Akt pathway, HMGA2 emerges as a promising prognostic biomarker and potential therapeutic target for glioma patients.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"272 ","pages":"Article 156051"},"PeriodicalIF":2.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomal circUBQLN1 facilitates M2 polarization of macrophages and enhances colorectal cancer progression by modulating the miR-34c-5p/CSF1R axis","authors":"Wei Wang ,&nbsp;Xuwei Ren ,&nbsp;Qinglong Chu ,&nbsp;Wei Wang ,&nbsp;Yuqin Quan ,&nbsp;Ling Zhang ,&nbsp;Xun Zhang","doi":"10.1016/j.prp.2025.156049","DOIUrl":"10.1016/j.prp.2025.156049","url":null,"abstract":"<div><div>While it has been established that exosomal circRNAs play a crucial role in facilitating communication between tumor cells and macrophages, there remains limited understanding regarding their specific functions and mechanisms related to both macrophage polarization as well as colorectal cancer (CRC) development. Verification for presence of CRC-derived Exo involved utilization of transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), as well as western blotting techniques. Uptake assessment for these Exo by TAMs was conducted using tracer analysis methods while determining precise roles played by circulating UBQLN1-containing Exo involved CCK-8 assays, Transwell experiments along with in vivo imaging studies on mice models. A significant increase was observed in expression levels for circUBQLN1 within CRC-derived Exo which could then be internalized by TAMs leading to induction towards an M2-like phenotype among them. Mechanistically this process involves regulation via miR-34c-5p/CSFIR signaling axis resulting ultimately into promotion for proliferation alongside metastasis among CRC cell populations. Exo-circUBQLN1 was identified as a critical mediator that promoted intercellular communication between the CRC cells and TAMs. Exo-circUBQLN1 caused M2-like phenotypic changes in the TAMs, while also encouraging the growth and spread of CRC cells by regulating miR-34c-p/CSFIR axis.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"272 ","pages":"Article 156049"},"PeriodicalIF":2.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gleason score down and upgrading at radical prostatectomy in targeted vs. systematic prostate biopsy: Findings from an institutional cohort","authors":"Vincenzo Fiorentino ,&nbsp;Ludovica Pepe ,&nbsp;Valeria Zuccalà ,&nbsp;Cristina Pizzimenti ,&nbsp;Antonio Ieni ,&nbsp;Maurizio Martini ,&nbsp;Mara Curduman ,&nbsp;Pietro Pepe","doi":"10.1016/j.prp.2025.156040","DOIUrl":"10.1016/j.prp.2025.156040","url":null,"abstract":"<div><h3>Background</h3><div>Accurate Gleason score (GS)/International Society of Urological Pathology (ISUP) Grade Group (GG) assessment in prostate cancer (PCa) is crucial for risk stratification and treatment. Multiparametric magnetic resonance imaging (mpMRI) and targeted biopsies (TPBx) have enhanced PCa detection, but their accuracy compared to systematic biopsies (SPBx) is under discussion. This study investigates GS/GG concordance between prostate biopsies (TPBx and SPBx) and radical prostatectomy (RP) specimens, evaluating rates and factors associated with GS/GG upgrading and downgrading.</div></div><div><h3>Materials and Methods</h3><div>A retrospective analysis of 100 patients with PI-RADS score <u>&gt;</u> 3 lesions</div><div>who underwent SPBx, with or without TPBx, followed by RP for clinically significant prostate cancer (csPCa) was performed.</div></div><div><h3>Results</h3><div>csPCa diagnosis was made by SPBx alone in 9/100 (9 %) cases, TPBx alone in 1/100 (1 %), and TPBx combined with SPBx in 90/100 (90 %). In the TPBx group, 76/90 (84.4 %) patients presented concordant GS/GG between biopsy and RP, while 14/90 (15.6 %) showed lower GS/GG at RP. In the SPBx group, 88/90 (97.8 %) presented concordant GS/GG, while 2/90 (2.2 %) showed higher GS at RP.</div></div><div><h3>Conclusions</h3><div>Our study highlights potential downgrading risk associated with TPBx alone, particularly in patients with initial GS of 4 + 4/GG4. While this has minimal implications for high-risk PCa, it raises concerns about potential overdiagnosis and overtreatment in men eligible for active surveillance (AS) who may be downgraded from intermediate-risk to low-risk or favourable intermediate-risk categories. This underscores the importance of using both TPBx and SPBx for PCa diagnosis and risk assessment.</div></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":"271 ","pages":"Article 156040"},"PeriodicalIF":2.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}