Expression of TIM-3 in transitional cell carcinoma: A comparative study of tissue and serum levels

IF 2.9 4区医学 Q2 PATHOLOGY

Pathology, research and practice Pub Date : 2025-05-23 DOI:10.1016/j.prp.2025.156031

Farrukh Siddique, Naveed Sharif, Abdul Salam, Saleha Saeed, Ayesha Qadir, Ayesha Siffat

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引用次数: 0

Abstract

Objective

To evaluate the expression of TIM-3 in tissue and serum specimens of patients diagnosed with transitional cell carcinoma (TCC), and to assess the correlation between local tissue expression and systemic serum levels.

Material and method

A cross-sectional study was conducted at Khyber Medical University, Peshawar, Pakistan. A total of 74 tissue samples (49 TCC cases, 25 controls) and 42 serum samples (35 TCC, 7 controls) were analyzed based on non-probability convenient sampling. Tissue TIM-3 expression was assessed using immunohistochemistry (IHC), while soluble TIM-3 (sTIM-3) concentrations in serum were measured by enzyme-linked immunosorbent assay ELISA.

Results

TIM-3 expression was found to be significantly higher in TCC tissue samples compared to controls (p < 0.001), and was associated with both higher tumor grade (p = 0.040) and stage (p = 0.008). Serum sTIM-3 concentrations were slightly elevated in TCC patients compared to healthy individuals, however, the difference was not statistically significant (p = 0.449). Also, no meaningful correlation was found between tissue TIM-3 expression and serum sTIM-3 levels (ρ = −0.119, p = 0.494).

Conclusion

Elevated TIM-3 expression in tumor tissue correlates with aggressive features of TCC, supporting its utility as a potential tissue-based biomarker. Serum sTIM-3, however, lacks diagnostic reliability and does not reflect tissue expression.

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TIM-3在移行细胞癌中的表达：组织和血清水平的比较研究

目的探讨TIM-3在移行细胞癌（TCC）患者组织和血清中的表达情况，并探讨其局部组织表达与全身血清水平的相关性。材料与方法在巴基斯坦白沙瓦开伯尔医科大学进行了一项横断面研究。采用非概率方便抽样法对74份组织样本（49例TCC， 25例对照）和42份血清样本（35例TCC， 7例对照）进行分析。采用免疫组化法（IHC）检测组织中TIM-3的表达，采用酶联免疫吸附法（ELISA）检测血清中可溶性TIM-3 （sTIM-3）的浓度。结果TCC组织样本中tstim -3的表达明显高于对照组（p <； 0.001），且与肿瘤分级（p = 0.040）和分期（p = 0.008）相关。TCC患者血清sTIM-3浓度较健康人群略有升高，但差异无统计学意义（p = 0.449）。组织中TIM-3表达与血清中sTIM-3水平无显著相关（ρ = - 0.119, p = 0.494）。结论TIM-3在肿瘤组织中的表达升高与TCC的侵袭性特征相关，支持其作为潜在的组织生物标志物的应用。然而，血清sTIM-3缺乏诊断可靠性，不能反映组织表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pathology, research and practice 医学-病理学

CiteScore

5.00

自引率

3.60%

发文量

405

审稿时长

24 days

期刊介绍： Pathology, Research and Practice provides accessible coverage of the most recent developments across the entire field of pathology: Reviews focus on recent progress in pathology, while Comments look at interesting current problems and at hypotheses for future developments in pathology. Original Papers present novel findings on all aspects of general, anatomic and molecular pathology. Rapid Communications inform readers on preliminary findings that may be relevant for further studies and need to be communicated quickly. Teaching Cases look at new aspects or special diagnostic problems of diseases and at case reports relevant for the pathologist''s practice.