Pathology International最新文献_第4页

Clinical significance of direct fast scarlet staining on the diagnosis of eosinophilic colitis: A comparative study focusing on the eosinophil degranulation in colonic mucosal tissue 直接快速猩红染色对诊断嗜酸性粒细胞结肠炎的临床意义：以结肠粘膜组织中嗜酸性粒细胞脱颗粒为重点的比较研究

IF 2.2 4区医学

Pathology International Pub Date : 2024-09-11 DOI: 10.1111/pin.13475

Mina Ikeda, Hiroyuki Kato, Satoshi Arakawa, Takashi Kobayashi, Senju Hashimoto, Yoshiaki Katano, Ken‐ichi Inada, Yuka Kiriyama, Takuma Ishihara, Satoshi Yamamoto, Yukio Asano, Akihiko Horiguchi

{"title":"Clinical significance of direct fast scarlet staining on the diagnosis of eosinophilic colitis: A comparative study focusing on the eosinophil degranulation in colonic mucosal tissue","authors":"Mina Ikeda, Hiroyuki Kato, Satoshi Arakawa, Takashi Kobayashi, Senju Hashimoto, Yoshiaki Katano, Ken‐ichi Inada, Yuka Kiriyama, Takuma Ishihara, Satoshi Yamamoto, Yukio Asano, Akihiko Horiguchi","doi":"10.1111/pin.13475","DOIUrl":"https://doi.org/10.1111/pin.13475","url":null,"abstract":"This study aimed to validate the DFS (direct fast scarlet) staining in the diagnosis of EC (eosinophilic colitis). The study included 50 patients with EC and 60 with control colons. Among the 60 control samples, 39 and 21 were collected from the ascending and descending colons, respectively. We compared the median number of eosinophils and frequency of eosinophil degranulation by HE (hematoxylin and eosin) and DFS staining between the EC and control groups. In the right hemi‐colon, eosinophil count by HE was useful in distinguishing between EC and control (41.5 vs. 26.0 cells/HPF, p < 0.001), but the ideal cutoff value is 27.5 cells/HPF (high‐power field). However, this method is not useful in the left hemi‐colon (12.5 vs. 13.0 cells/HPF, p = 0.990). The presence of degranulation by DFS allows us to distinguish between the groups even in the left hemi‐colon (58% vs. 5%, p < 0.001). DFS staining also enabled a more accurate determination of degranulation than HE. According to the current standard to diagnose EC (count by HE staining ≥20 cells/HPF), mucosal sampling from left hemi‐colon is problematic since the number of eosinophils could not be increased even in EC. Determination of degranulated eosinophils by DFS may potentiate the diagnostic performance even in such conditions.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":"6 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Giant inflammatory polyposis in Crohn's disease mimicking recurrent obstructing colon cancer 模仿复发性梗阻性结肠癌的克罗恩病巨型炎性息肉病

IF 2.2 4区医学

Pathology International Pub Date : 2024-09-10 DOI: 10.1111/pin.13479

Christopher Holt, David E. Elliott, Yiqin Xiong

引用次数: 0

Standardization of CD30 immunohistochemistry staining among three automated immunostaining platforms. 三种自动免疫染色平台的 CD30 免疫组化染色标准化。

IF 2.5 4区医学

Pathology International Pub Date : 2024-09-01 Epub Date: 2024-08-22 DOI: 10.1111/pin.13472

Masafumi Seki, Akira Satou, Renji Funato, Tomoko Tamaki, Naoki Wada, Norihiro Nakada, Hirofumi Matsumoto, Iwao Nakazato, Eriko Wada, Kaneko Sakurai, Toyonori Tsuzuki, Kennosuke Karube

{"title":"Standardization of CD30 immunohistochemistry staining among three automated immunostaining platforms.","authors":"Masafumi Seki, Akira Satou, Renji Funato, Tomoko Tamaki, Naoki Wada, Norihiro Nakada, Hirofumi Matsumoto, Iwao Nakazato, Eriko Wada, Kaneko Sakurai, Toyonori Tsuzuki, Kennosuke Karube","doi":"10.1111/pin.13472","DOIUrl":"10.1111/pin.13472","url":null,"abstract":"The identification of CD30 expression by immunohistochemistry is essential for the treatment of lymphomas using an antibody-drug conjugate targeting CD30. However, no standardized protocol for CD30 staining has been available. In this study, we compared three common automated immunostaining platforms {Bond III (B III), Dako Omnis (DO) and Ventana BenchMark ULTRA (VBMU)}. A primary antibody for CD30, the Ber-H2 clone, was diluted 50- to 400-fold for B III and DO, and ready-to-use antibody was used for VBMU. An enhancement step using a linker was introduced in all protocols. First, several candidate dilutions were selected for each platform by staining six cases. These candidate conditions were then confirmed with 60 cases of various types of peripheral T-cell lymphomas (PTCLs). The concordance rates of CD30 expression among platforms differed depending on cutoff values and antibody dilutions, except for anaplastic large cell lymphoma. The concordance rates among three platforms in the evaluation of \"positive\" or \"negative\" were 100% and 97% when the cutoff values were 1% and 10% respectively, if using 400-diluted antibody in B III and 100-diluted antibody in DO. This study demonstrated the feasibility of equalizing CD30 staining of PTCLs among different platforms by adjusting protocols.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":" ","pages":"530-537"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142018268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-calcifying Langerhans cell-rich myxoid squamous odontogenic neoplasm without amyloid: A seemingly amyloid-negative calcifying epithelial odontogenic tumor. 不含淀粉样蛋白的非钙化朗格汉斯细胞丰富肌样鳞状牙源性肿瘤：一种看似淀粉样蛋白阴性的钙化上皮性牙源性肿瘤。

IF 2.5 4区医学

Pathology International Pub Date : 2024-09-01 Epub Date: 2024-07-31 DOI: 10.1111/pin.13470

Fumio Ide, Shinnichi Sakamoto, Yoko Tateishi, Hiroyuki Hayashi, Takayuki Ohsawa, Yumi Ito, Kentaro Kikuchi

引用次数: 0

FHL1: A novel diagnostic marker for papillary thyroid carcinoma. FHL1：甲状腺乳头状癌的新型诊断标记物。

IF 2.5 4区医学

Pathology International Pub Date : 2024-09-01 Epub Date: 2024-08-09 DOI: 10.1111/pin.13467

Yeting Zeng, Dehua Zeng, Xingfeng Qi, Hanxi Wang, Xuzhou Wang, Xiaodong Dai, Lijuan Qu

{"title":"FHL1: A novel diagnostic marker for papillary thyroid carcinoma.","authors":"Yeting Zeng, Dehua Zeng, Xingfeng Qi, Hanxi Wang, Xuzhou Wang, Xiaodong Dai, Lijuan Qu","doi":"10.1111/pin.13467","DOIUrl":"10.1111/pin.13467","url":null,"abstract":"Although there are clear morphologic criteria for the diagnosis of papillary thyroid carcinoma (PTC), when the morphology is untypical or overlaps, accurate diagnostic indicators are necessary. Since few studies investigated the role of down-regulated genes in PTC, this article aims to further explore the molecular markers associated with PTC. We conducted bioinformatics analysis of gene microarrays of PTC and normal adjacent tissues. Besides, quantitative real-time quantitative polymerase chain reaction array and immunohistochemical staining were used to investigate the expression of the major down-regulated genes. The results indicated that several important down-regulated genes, including TLE1, BCL2, FHL1, GHR, KIT, and PPARGC1A were involved in the process of PTC. Compared to normal adjacent tissues, the mRNA expression of the major genes was down-regulated in PTC (p＜0.05). Immunohistochemically, FHL1 shows negative or low expression in PTC tissues (p＜0.05). BCL2 did not show a significant difference between PTC and normal thyroid tissues (p > 0.05). TLE1, KIT, PPARGC1A and GHR showed negative expression in both tumor and normal tissues. These results suggested that FHL1 could serve as a novel tumor marker for precise diagnosis of PTC.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":" ","pages":"520-529"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New insights into cancer pathology learned from the dynamics of cancer-associated fibroblasts. 从癌症相关成纤维细胞的动态中了解癌症病理学的新见解。

IF 2.5 4区医学

Pathology International Pub Date : 2024-09-01 Epub Date: 2024-06-25 DOI: 10.1111/pin.13461

Genichiro Ishii

{"title":"New insights into cancer pathology learned from the dynamics of cancer-associated fibroblasts.","authors":"Genichiro Ishii","doi":"10.1111/pin.13461","DOIUrl":"10.1111/pin.13461","url":null,"abstract":"Paget's \"Seed and Soil\" theory, proposed in 1889, emphasizes the importance of the microenvironment where cancer cells grow in metastatic sites. Over a century later, this concept remains a cornerstone in comprehending cancer biology and devising treatment strategies. The \"Seed and Soil\" theory, which initially explained how cancer spreads to distant organs, now also applies to the tumor microenvironment (TME) within primary tumors. This theory emphasizes the critical interaction between cancer cells (\"seeds\") and their surrounding environment (\"soil\") and how this interaction affects both tumor progression within the primary site and at metastatic sites. An important point to note is that the characteristics of the TME are not static but dynamic, undergoing substantial changes during tumor progression and after treatment with therapeutic drugs. Cancer-associated fibroblasts (CAFs), recognized as the principal noncancerous cellular component within the TME, play multifaceted roles in tumor progression including promoting angiogenesis, remodeling the extracellular matrix, and regulating immune responses. In this comprehensive review, we focus on the findings regarding how the dynamics of CAFs contribute to cancer progression and drug sensitivity. Understanding the dynamics of CAFs could provide new insights into cancer pathology and lead to important advancements in cancer research and treatment.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":" ","pages":"493-507"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141458533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A case of hepatoid adenocarcinoma of the lung harboring KRAS G12C responded favorably to sotorasib. 一例携带 KRAS G12C 的肺肝样腺癌患者对索托拉西布治疗反应良好。

IF 2.5 4区医学

Pathology International Pub Date : 2024-09-01 Epub Date: 2024-07-17 DOI: 10.1111/pin.13464

Hironori Uruga, Atsushi Miyamoto, Takeshi Fujii, Takashi Okuma, Takahiro Mitsumura, Meiyo Tamaoka, Yutaka Takazawa

{"title":"A case of hepatoid adenocarcinoma of the lung harboring KRAS G12C responded favorably to sotorasib.","authors":"Hironori Uruga, Atsushi Miyamoto, Takeshi Fujii, Takashi Okuma, Takahiro Mitsumura, Meiyo Tamaoka, Yutaka Takazawa","doi":"10.1111/pin.13464","DOIUrl":"10.1111/pin.13464","url":null,"abstract":"Hepatoid adenocarcinoma of the lung is a rare variant of adenocarcinoma. We describe a case of hepatoid adenocarcinoma of the lung that harbored KRAS G12C and responded favorably to sotorasib. A man in his 70s was found to have an abnormality on his chest X-ray. He underwent right middle lobectomy, and a pathological examination of the surgical specimen showed conventional invasive adenocarcinoma with highly focal hepatoid adenocarcinoma. He received chemoradiotherapy and concurrent radiation, followed by durvalumab for postoperative recurrence. After three doses of durvalumab, he reported feeling short of breath. A computed tomography scan showed emerging broad consolidation in the right lower lobe. Transbronchial lung biopsy specimens from the consolidation showed hepatoid adenocarcinoma harboring KRAS G12C mutation. Therefore, he was started on sotorasib 960 mg daily. Eight days later, a computed tomography scan showed that the area of consolidation had reduced in size. Progressive disease was detected after 42 days of treatment with sotorasib. The patient died 1 month after cessation of sotorasib and 3 months after postoperative recurrence. We have encountered what we believe to be the first case of hepatoid adenocarcinoma of the lung with KRAS G12C mutation that responded favorably to treatment with sotorasib.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":" ","pages":"546-551"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141627308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digital whole-slide imaging of changes in amyloid after peripheral blood stem cell transplantation in patients with amyloid light-chain amyloidosis. 淀粉样轻链淀粉样变性病患者外周血干细胞移植后淀粉样蛋白变化的数字全切片成像。

IF 2.5 4区医学

Pathology International Pub Date : 2024-09-01 Epub Date: 2024-07-17 DOI: 10.1111/pin.13466

Kei Kono, Naoki Sawa, Atsushi Wake, Yukako Shintani-Domoto, Takeshi Fujii, Yutaka Takazawa, Yoshifumi Ubara, Kenichi Ohashi

{"title":"Digital whole-slide imaging of changes in amyloid after peripheral blood stem cell transplantation in patients with amyloid light-chain amyloidosis.","authors":"Kei Kono, Naoki Sawa, Atsushi Wake, Yukako Shintani-Domoto, Takeshi Fujii, Yutaka Takazawa, Yoshifumi Ubara, Kenichi Ohashi","doi":"10.1111/pin.13466","DOIUrl":"10.1111/pin.13466","url":null,"abstract":"Peripheral blood stem cell transplantation (PBSCT) has made amyloid light-chain (AL) amyloidosis treatable. After PBSCT, hematological complete remission (HCR) can be achieved, leading to improved renal prognosis. The purpose of this study was to evaluate whether whole slide imaging of biopsy samples shows a post-treatment reduction in amyloid deposits in patients with AL amyloidosis. Patients were divided into three groups: Group A (n = 8), not eligible for PBSCT and treated with other therapies; Group B (n = 11), treated with PBSCT and achieved HCR; and Group C (n = 5), treated with PBSCT but did not achieve HCR. Clinical findings and amyloid deposition in glomeruli, interstitium, and blood vessels were compared before and after treatment using digital whole-slide imaging. Proteinuria and hypoalbuminemia improved more in Group B than in the other groups, and in Group B, amyloid deposition improved more in the glomeruli than in the interstitium and blood vessels. The long-term renal and survival prognosis was better in Group B than in the other groups. PBSCT can be expected to improve long-term clinical and renal histological prognosis in patients with AL amyloidosis who achieve HCR. Amyloid disappearance from renal tissue may take a long time even after clinical HCR.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":" ","pages":"508-519"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141627309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autopsy case of linear nevus sebaceous syndrome with KRAS (G12D) mutation. KRAS（G12D）突变的线性皮脂腺痣综合征尸检病例。

IF 2.5 4区医学

Pathology International Pub Date : 2024-09-01 Epub Date: 2024-07-12 DOI: 10.1111/pin.13463

Akira Ohishi, Yasunori Enomoto, Hideto Iwafuchi, Shiori Meguro, Isao Kosugi, Satoshi Baba, Toshihide Iwashita, Yuki Segawa, Daizo Ueno, Shigeo Iijima

{"title":"Autopsy case of linear nevus sebaceous syndrome with KRAS (G12D) mutation.","authors":"Akira Ohishi, Yasunori Enomoto, Hideto Iwafuchi, Shiori Meguro, Isao Kosugi, Satoshi Baba, Toshihide Iwashita, Yuki Segawa, Daizo Ueno, Shigeo Iijima","doi":"10.1111/pin.13463","DOIUrl":"10.1111/pin.13463","url":null,"abstract":"Linear nevus sebaceous syndrome (LNSS) is a neurocutaneous syndrome associated with systemic complications that involve multiple organs, including the skin, central nervous system, eyes, and skeleton. LNSS is considered to be caused by mosaic RAS gene mutation. In this report, we present an autopsy case of LNSS in a Japanese boy. The affected neonate had hydrops fetalis and was born at 28 weeks and 4 days of gestation, weighing 2104 g. He had bilateral inverted eyelids, verrucous linear nevus separated along Blaschko's line, myocardial hypertrophy, and pharyngeal constriction, and underwent intensive treatment in NICU for arrhythmia, hydrocephalus, and respiratory distress. The hydrocephalus progressed gradually and he died at the age of 181 days, 12 days after a sudden cardiac arrest and recovery. KRAS G12D mutation was found in a skin biopsy specimen but not in blood cells, suggesting a postzygotic mosaicism. Autopsy revealed novel pathological findings related to LNSS, including intracranial lipomatous hamartoma and mesenteric lymphangioma, in addition to previously reported findings such as multicystic dysplastic kidney. There was the limited expression of mutated KRAS protein in kidneys.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":" ","pages":"538-545"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathological evaluation of the pathogenesis of diabetes mellitus and diabetic peripheral neuropathy. 对糖尿病和糖尿病周围神经病变发病机制的病理学评估。

IF 2.5 4区医学

Pathology International Pub Date : 2024-08-01 Epub Date: 2024-06-18 DOI: 10.1111/pin.13458

Hiroki Mizukami

{"title":"Pathological evaluation of the pathogenesis of diabetes mellitus and diabetic peripheral neuropathy.","authors":"Hiroki Mizukami","doi":"10.1111/pin.13458","DOIUrl":"10.1111/pin.13458","url":null,"abstract":"Currently, there are more than 10 million patients with diabetes mellitus in Japan. Therefore, the need to explore the pathogenesis of diabetes and the complications leading to its cure is becoming increasingly urgent. Pathological examination of pancreatic tissues from patients with type 2 diabetes reveals a decrease in the volume of beta cells because of a combination of various stresses. In human type 2 diabetes, islet amyloid deposition is a unique pathological change characterized by proinflammatory macrophage (M1) infiltration into the islets. The pathological changes in the pancreas with islet amyloid were different according to clinical factors, which suggests that type 2 diabetes can be further subclassified based on islet pathology. On the other hand, diabetic peripheral neuropathy is the most frequent diabetic complication. In early diabetic peripheral neuropathy, M1 infiltration in the sciatic nerve evokes oxidative stress or attenuates retrograde axonal transport, as clearly demonstrated by in vitro live imaging. Furthermore, islet parasympathetic nerve density and beta cell volume were inversely correlated in type 2 diabetic Goto-Kakizaki rats, suggesting that diabetic peripheral neuropathy itself may contribute to the decrease in beta cell volume. These findings suggest that the pathogenesis of diabetes mellitus and diabetic peripheral neuropathy may be interrelated.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":" ","pages":"438-453"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141420275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0