Pathology International最新文献_第3页

Fluorescence in situ hybridization-negative intra-articular myxoid liposarcoma with complex rearrangements involving EWSR1::DDIT3 detected using nanopore sequencing. 利用纳米孔测序法检测到荧光原位杂交阴性的关节内肌样脂肪肉瘤，其中有涉及 EWSR1::DDIT3 的复杂重排。

IF 2.5 4区医学

Pathology International Pub Date : 2024-07-29 DOI: 10.1111/pin.13468

Naohiro Makise, Jason Lin, Hajime Kageyama, Naoki Takeda, Mariko Oikawa, Takahiro Sugiyama, Hidetada Kawana, Akinobu Araki, Hideyuki Kinoshita, Hiroto Kamoda, Yoko Hagiwara, Akihiko Yoshida, Tsukasa Yonemoto, Masahito Kawazu, Makiko Itami

{"title":"Fluorescence in situ hybridization-negative intra-articular myxoid liposarcoma with complex rearrangements involving EWSR1::DDIT3 detected using nanopore sequencing.","authors":"Naohiro Makise, Jason Lin, Hajime Kageyama, Naoki Takeda, Mariko Oikawa, Takahiro Sugiyama, Hidetada Kawana, Akinobu Araki, Hideyuki Kinoshita, Hiroto Kamoda, Yoko Hagiwara, Akihiko Yoshida, Tsukasa Yonemoto, Masahito Kawazu, Makiko Itami","doi":"10.1111/pin.13468","DOIUrl":"https://doi.org/10.1111/pin.13468","url":null,"abstract":"Myxoid liposarcoma (MLPS) is a rare sarcoma, typically arising in deep soft tissues during the fourth to fifth decades of life. Histologically, MLPS is composed of uniform oval cells within a background of myxoid stroma and chicken-wire capillaries. Genetically, MLPS is characterized by the FUS/EWSR1::DDIT3 fusion gene, which generally results from balanced interchromosomal translocation and is detectable via DDIT3 break-apart fluorescence in situ hybridization (FISH). Here, we report an unusual intra-articular MLPS case, negative for DDIT3 break-apart FISH but positive for EWSR1::DDIT3. An 18-year-old female was referred to our hospital complaining of an intra-articular mass in the right knee joint. Histologically, the tumor was mainly composed of mature adipocytes, brown fat-like cells, and lipoblasts. Nanopore sequencing detected DNA rearrangements between EWSR1 and DDIT3 and clustered complex rearrangements involving multiple chromosomes, suggesting chromoplexy. Methylation classification using random forest, t-distributed stochastic neighbor embedding, and unsupervised hierarchical clustering correctly classified the tumor as MLPS. The copy number was almost flat. The TERT promoter C-124T was also detected. This report highlights, for the first time, the potential value of a fast and low-cost nanopore sequencer for diagnosing sarcomas.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathology of peritonitis due to infectious mononucleosis with background Chlamydia trachomatis infection: A case report and literature review. 传染性单核细胞增多症合并沙眼衣原体感染所致腹膜炎的组织病理学：病例报告和文献综述。

IF 2.5 4区医学

Pathology International Pub Date : 2024-07-26 DOI: 10.1111/pin.13469

Yuto Kaimi, Tomoaki Naka, Hiroshi Yoshida, Masaya Uno, Akiko Miyagi Maeshima

{"title":"Histopathology of peritonitis due to infectious mononucleosis with background Chlamydia trachomatis infection: A case report and literature review.","authors":"Yuto Kaimi, Tomoaki Naka, Hiroshi Yoshida, Masaya Uno, Akiko Miyagi Maeshima","doi":"10.1111/pin.13469","DOIUrl":"https://doi.org/10.1111/pin.13469","url":null,"abstract":"Epstein-Barr virus (EBV) is a major cause of infectious mononucleosis (IM), characterized by fever, fatigue, sore throat, lymphadenopathy, atypical lymphocytosis, and elevated liver enzymes. However, ascites is a rare complication associated with IM. We present a rare case of IM with ascites and peritonitis in a patient who underwent a peritoneal biopsy. A 20-year-old woman presented with fatigue and abdominal distension. Laboratory examination revealed atypical lymphocytes in peripheral blood (54%) and elevated liver enzymes. EBV serological tests revealed a recent primary infection (EBV VCA IgM 1:160). Computed tomography revealed moderate ascites and peritonitis. Adenocarcinoma was suspected based on the ascites' cytology. Considering possible complications of IM and adenocarcinoma, a laparoscopic biopsy was performed. Histological findings of biopsy specimens from the peritoneum, omentum, and fimbria of the fallopian tube demonstrated severe inflammatory cell infiltration and focal aggregation of large EBV-encoded RNA-1 (EBER1)-positive B cells, mimicking EBV-positive polymorphous B-cell lymphoproliferative disorder. Furthermore, intracytoplasmic inclusion bodies of Chlamydia trachomatis were observed by immunohistochemistry. Real-time polymerase chain reaction detected C. trachomatis in cervical secretions. Two months after laparoscopy, ascites decreased, and the diagnosis was IM-associated peritonitis with C. trachomatis infection. IM should be considered as a differential diagnosis in young patients with ascites.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A case of hepatoid adenocarcinoma of the lung harboring KRAS G12C responded favorably to sotorasib. 一例携带 KRAS G12C 的肺肝样腺癌患者对索托拉西布治疗反应良好。

IF 2.5 4区医学

Pathology International Pub Date : 2024-07-17 DOI: 10.1111/pin.13464

Hironori Uruga, Atsushi Miyamoto, Takeshi Fujii, Takashi Okuma, Takahiro Mitsumura, Meiyo Tamaoka, Yutaka Takazawa

{"title":"A case of hepatoid adenocarcinoma of the lung harboring KRAS G12C responded favorably to sotorasib.","authors":"Hironori Uruga, Atsushi Miyamoto, Takeshi Fujii, Takashi Okuma, Takahiro Mitsumura, Meiyo Tamaoka, Yutaka Takazawa","doi":"10.1111/pin.13464","DOIUrl":"https://doi.org/10.1111/pin.13464","url":null,"abstract":"Hepatoid adenocarcinoma of the lung is a rare variant of adenocarcinoma. We describe a case of hepatoid adenocarcinoma of the lung that harbored KRAS G12C and responded favorably to sotorasib. A man in his 70s was found to have an abnormality on his chest X-ray. He underwent right middle lobectomy, and a pathological examination of the surgical specimen showed conventional invasive adenocarcinoma with highly focal hepatoid adenocarcinoma. He received chemoradiotherapy and concurrent radiation, followed by durvalumab for postoperative recurrence. After three doses of durvalumab, he reported feeling short of breath. A computed tomography scan showed emerging broad consolidation in the right lower lobe. Transbronchial lung biopsy specimens from the consolidation showed hepatoid adenocarcinoma harboring KRAS G12C mutation. Therefore, he was started on sotorasib 960 mg daily. Eight days later, a computed tomography scan showed that the area of consolidation had reduced in size. Progressive disease was detected after 42 days of treatment with sotorasib. The patient died 1 month after cessation of sotorasib and 3 months after postoperative recurrence. We have encountered what we believe to be the first case of hepatoid adenocarcinoma of the lung with KRAS G12C mutation that responded favorably to treatment with sotorasib.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141627308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digital whole-slide imaging of changes in amyloid after peripheral blood stem cell transplantation in patients with amyloid light-chain amyloidosis. 淀粉样轻链淀粉样变性病患者外周血干细胞移植后淀粉样蛋白变化的数字全切片成像。

IF 2.5 4区医学

Pathology International Pub Date : 2024-07-17 DOI: 10.1111/pin.13466

Kei Kono, Naoki Sawa, Atsushi Wake, Yukako Shintani-Domoto, Takeshi Fujii, Yutaka Takazawa, Yoshifumi Ubara, Kenichi Ohashi

{"title":"Digital whole-slide imaging of changes in amyloid after peripheral blood stem cell transplantation in patients with amyloid light-chain amyloidosis.","authors":"Kei Kono, Naoki Sawa, Atsushi Wake, Yukako Shintani-Domoto, Takeshi Fujii, Yutaka Takazawa, Yoshifumi Ubara, Kenichi Ohashi","doi":"10.1111/pin.13466","DOIUrl":"https://doi.org/10.1111/pin.13466","url":null,"abstract":"Peripheral blood stem cell transplantation (PBSCT) has made amyloid light-chain (AL) amyloidosis treatable. After PBSCT, hematological complete remission (HCR) can be achieved, leading to improved renal prognosis. The purpose of this study was to evaluate whether whole slide imaging of biopsy samples shows a post-treatment reduction in amyloid deposits in patients with AL amyloidosis. Patients were divided into three groups: Group A (n = 8), not eligible for PBSCT and treated with other therapies; Group B (n = 11), treated with PBSCT and achieved HCR; and Group C (n = 5), treated with PBSCT but did not achieve HCR. Clinical findings and amyloid deposition in glomeruli, interstitium, and blood vessels were compared before and after treatment using digital whole-slide imaging. Proteinuria and hypoalbuminemia improved more in Group B than in the other groups, and in Group B, amyloid deposition improved more in the glomeruli than in the interstitium and blood vessels. The long-term renal and survival prognosis was better in Group B than in the other groups. PBSCT can be expected to improve long-term clinical and renal histological prognosis in patients with AL amyloidosis who achieve HCR. Amyloid disappearance from renal tissue may take a long time even after clinical HCR.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141627309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autopsy case of linear nevus sebaceous syndrome with KRAS (G12D) mutation. KRAS（G12D）突变的线性皮脂腺痣综合征尸检病例。

IF 2.5 4区医学

Pathology International Pub Date : 2024-07-12 DOI: 10.1111/pin.13463

Akira Ohishi, Yasunori Enomoto, Hideto Iwafuchi, Shiori Meguro, Isao Kosugi, Satoshi Baba, Toshihide Iwashita, Yuki Segawa, Daizo Ueno, Shigeo Iijima

{"title":"Autopsy case of linear nevus sebaceous syndrome with KRAS (G12D) mutation.","authors":"Akira Ohishi, Yasunori Enomoto, Hideto Iwafuchi, Shiori Meguro, Isao Kosugi, Satoshi Baba, Toshihide Iwashita, Yuki Segawa, Daizo Ueno, Shigeo Iijima","doi":"10.1111/pin.13463","DOIUrl":"https://doi.org/10.1111/pin.13463","url":null,"abstract":"Linear nevus sebaceous syndrome (LNSS) is a neurocutaneous syndrome associated with systemic complications that involve multiple organs, including the skin, central nervous system, eyes, and skeleton. LNSS is considered to be caused by mosaic RAS gene mutation. In this report, we present an autopsy case of LNSS in a Japanese boy. The affected neonate had hydrops fetalis and was born at 28 weeks and 4 days of gestation, weighing 2104 g. He had bilateral inverted eyelids, verrucous linear nevus separated along Blaschko's line, myocardial hypertrophy, and pharyngeal constriction, and underwent intensive treatment in NICU for arrhythmia, hydrocephalus, and respiratory distress. The hydrocephalus progressed gradually and he died at the age of 181 days, 12 days after a sudden cardiac arrest and recovery. KRAS G12D mutation was found in a skin biopsy specimen but not in blood cells, suggesting a postzygotic mosaicism. Autopsy revealed novel pathological findings related to LNSS, including intracranial lipomatous hamartoma and mesenteric lymphangioma, in addition to previously reported findings such as multicystic dysplastic kidney. There was the limited expression of mutated KRAS protein in kidneys.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leucine-rich repeat-containing G protein-coupled receptor 5 expression in lymph node metastases of colorectal cancer: Clinicopathological insights and prognostic implications. 结直肠癌淋巴结转移中富含亮氨酸重复的 G 蛋白偶联受体 5 的表达：临床病理学见解和预后意义。

IF 2.5 4区医学

Pathology International Pub Date : 2024-07-01 Epub Date: 2024-05-24 DOI: 10.1111/pin.13439

Hiroshi Sawaguchi, Takeshi Uehara, Mai Iwaya, Shiho Asaka, Tomoyuki Nakajima, Masato Kamakura, Tadanobu Nagaya, Takahiro Yoshizawa, Hiroyoshi Ota, Takeji Umemura

{"title":"Leucine-rich repeat-containing G protein-coupled receptor 5 expression in lymph node metastases of colorectal cancer: Clinicopathological insights and prognostic implications.","authors":"Hiroshi Sawaguchi, Takeshi Uehara, Mai Iwaya, Shiho Asaka, Tomoyuki Nakajima, Masato Kamakura, Tadanobu Nagaya, Takahiro Yoshizawa, Hiroyoshi Ota, Takeji Umemura","doi":"10.1111/pin.13439","DOIUrl":"10.1111/pin.13439","url":null,"abstract":"Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), a significant cancer stem cell marker in colorectal cancer (CRC), lacks lymph node (LN) expression studies. In this study, we identified LGR5 expression by RNAscope, a highly sensitive RNA in situ method, and analyzed its association with clinicopathological characteristics. Tissue microarrays were generated from primary tumors (PTs) and LN metastases in paraffin-embedded blocks of 38 CRC surgical resection materials. LGR5 expression by RNAscope was evaluated by dividing the expression levels into negative and positive expression. In all but two cases of LN metastasis, LGR5-positive dots were detected in tumor cells, and there was a wide range of LGR5-positive cells. More LGR5-positive dots were identified in the gland-forming region. Twenty-three cases were classified into a high LGR5-expression group, and 15 cases were classified into a low LGR5-expression group. In the high LGR5-expression group, the histological grade was lower than in the low LGR5-expression group (p = 0.0159), while necrosis was significantly more prevalent (p = 0.0326), and the tumor, node, metastasis stage was significantly lower (p = 0.0302). There was no association between LGR5 expression levels in LN metastases and LGR5 expression levels in PT tissue. LGR5 expression in LN metastases may influence prognosis. Further analysis may lead to new therapeutic strategies.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141088458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autopsy report of a sudden infant death that was strongly suspicious of Kawasaki disease. 婴儿猝死的尸检报告，强烈怀疑是川崎病所致。

IF 2.5 4区医学

Pathology International Pub Date : 2024-07-01 Epub Date: 2024-05-15 DOI: 10.1111/pin.13437

Yuki Yokouchi, Nanae Asakawa, Hirotaro Iwase, Takeshi Nasu, Kei Takahashi

{"title":"Autopsy report of a sudden infant death that was strongly suspicious of Kawasaki disease.","authors":"Yuki Yokouchi, Nanae Asakawa, Hirotaro Iwase, Takeshi Nasu, Kei Takahashi","doi":"10.1111/pin.13437","DOIUrl":"10.1111/pin.13437","url":null,"abstract":"We conducted an autopsy on a 3-month-old boy in whom Kawasaki disease (KD) was strongly suspected based on the autopsy findings. The infant had a fever and was brought to a nearby clinic, where he was prescribed antipyretics and kept under observation. However, 15 days after onset of the fever, he suddenly died in bed. He exhibited no obvious redness of the lips, tongue, or conjunctiva. Membranous desquamation was present on his distal fingers. Vasculitis was observed in the coronary arteries, renal artery, splenic artery, and pulmonary vein. In addition, coronary artery aneurysms were present in the right coronary artery and left anterior descending artery. Thrombotic occlusion was observed in one aneurysm in the right coronary artery, resulting in acute myocardial infarction. The coronary artery wall showed infiltration of numerous macrophages and neutrophils. This case was classified as incomplete KD because the coronary artery aneurysm could not be demonstrated before death and was only recognized at autopsy. Pathologists and forensic scientists need to be aware that there are cases in which KD goes undiagnosed and untreated, leading to coronary artery aneurysm formation and sudden death.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140945344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial comment to "Autopsy report of a sudden infant death that was strongly suspicious of Kawasaki disease". 对 "强烈怀疑为川崎病的婴儿猝死验尸报告 "的编辑评论。

IF 2.5 4区医学

Pathology International Pub Date : 2024-07-01 Epub Date: 2024-05-23 DOI: 10.1111/pin.13440

Hirotake Masuda

引用次数: 0

Aberrant differentiation and proliferation of hepatocytes in chronic liver injury and liver tumors. 慢性肝损伤和肝肿瘤中肝细胞的异常分化和增殖。

IF 2.5 4区医学

Pathology International Pub Date : 2024-07-01 Epub Date: 2024-06-05 DOI: 10.1111/pin.13441

Yuji Nishikawa

{"title":"Aberrant differentiation and proliferation of hepatocytes in chronic liver injury and liver tumors.","authors":"Yuji Nishikawa","doi":"10.1111/pin.13441","DOIUrl":"10.1111/pin.13441","url":null,"abstract":"Chronic liver injury induces liver cirrhosis and facilitates hepatocarcinogenesis. However, the effects of this condition on hepatocyte proliferation and differentiation are unclear. We showed that rodent hepatocytes display a ductular phenotype when they are cultured within a collagenous matrix. This process involves transdifferentiation without the emergence of hepatoblastic features and is at least partially reversible. During the ductular reaction in chronic liver diseases with progressive fibrosis, some hepatocytes, especially those adjacent to ectopic ductules, demonstrate ductular transdifferentiation, but the majority of increased ductules originate from the existing bile ductular system that undergoes extensive remodeling. In chronic injury, hepatocyte proliferation is weak but sustained, and most regenerative nodules in liver cirrhosis are composed of clonally proliferating hepatocytes, suggesting that a small fraction of hepatocytes maintain their proliferative capacity in chronic injury. In mouse hepatocarcinogenesis models, hepatocytes activate the expression of various fetal/neonatal genes, indicating that these cells undergo dedifferentiation. Hepatocyte-specific somatic integration of various oncogenes in mice demonstrated that hepatocytes may be the cells of origin for a broad spectrum of liver tumors through transdifferentiation and dedifferentiation. In conclusion, the phenotypic plasticity and heterogeneity of mature hepatocytes are important for understanding the pathogenesis of chronic liver diseases and liver tumors.","PeriodicalId":19806,"journal":{"name":"Pathology International","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141262518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A modifiable valve-sparing pediatric cardiac dissection technique promotes specimen longevity and optimizes advanced image analysis postpathological examination. 可修改的小儿心脏瓣膜剥离技术可延长标本寿命，优化病理检查后的高级图像分析。

IF 2.5 4区医学

Pathology International Pub Date : 2024-07-01 Epub Date: 2024-05-07 DOI: 10.1111/pin.13435

Takato Yamasaki, Shuhei Toba, Stephen P Sanders, Chrystalle Katte Carreon

引用次数: 0