Pathogens最新文献_第3页

Identifying New Areas of Endemicity and Risk Factors for Rickettsia conorii subsp. conorii Infection: Serosurvey in Rural Areas of Romania 确定新的地方病流行区和 Conorii 亚种立克次体感染的风险因素：罗马尼亚农村地区血清调查

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-11 DOI: 10.3390/pathogens13090783

Cristina Alexandra Cheran, Andreea Madalina Panciu, Claudia Doina Riciu, Iulia Maria Nedelcu, Diana Gabriela Iacob, Adriana Hristea

{"title":"Identifying New Areas of Endemicity and Risk Factors for Rickettsia conorii subsp. conorii Infection: Serosurvey in Rural Areas of Romania","authors":"Cristina Alexandra Cheran, Andreea Madalina Panciu, Claudia Doina Riciu, Iulia Maria Nedelcu, Diana Gabriela Iacob, Adriana Hristea","doi":"10.3390/pathogens13090783","DOIUrl":"https://doi.org/10.3390/pathogens13090783","url":null,"abstract":"Background: Mediterranean spotted fever (MSF) is an emerging tick-borne disease caused by Rickettsia conorii subsp. conorii, primarily prevalent in Mediterranean and Southern Europe. We aimed to evaluate MSF seroprevalence and risk factors in non-endemic rural areas of Romania. Methods: We conducted a serosurvey in five counties not under MSF surveillance by testing 459 serum samples from adult volunteers for specific IgG antibodies using ELISA. Participants answered a questionnaire regarding demographics and MSF risk factors. Results: The median age of the participants was 60 years and 329 (71.7%) were female. Overall, 64 (13.9%) samples tested positive for IgG anti-R. conorii, with rates ranging from 7.1% in Sibiu to 22.4% in Hunedoara. The median age of the positive individuals was 68 years, with a significantly higher seropositivity rate of 54.7% among those over 65 years (p = 0.01). Among those positive, 53 (82.8%) owned different household animals; 24 (37.5%) had daily contact with dogs, and 27 (42.2%) with livestock; 17 (26.6%) noted tick infestations of animals, and 23 (35.9%) reported tick bites. Conclusions: This study revealed an important seroprevalence of MSF in Romanian areas considered non-endemic, indicating an expansion of its geographical range, probably due to climate change, and emphasizing the importance of enhanced surveillance and diagnostic capabilities nationwide.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inorganic Polyphosphate Is in the Surface of Trypanosoma cruzi but Is Not Significantly Secreted 无机聚磷酸盐存在于克鲁斯锥虫表面，但分泌量不大

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-09 DOI: 10.3390/pathogens13090776

Logan P. Crowe, Anna Gioseffi, Mayara S. Bertolini, Roberto Docampo

{"title":"Inorganic Polyphosphate Is in the Surface of Trypanosoma cruzi but Is Not Significantly Secreted","authors":"Logan P. Crowe, Anna Gioseffi, Mayara S. Bertolini, Roberto Docampo","doi":"10.3390/pathogens13090776","DOIUrl":"https://doi.org/10.3390/pathogens13090776","url":null,"abstract":"Trypanosoma cruzi is the etiologic agent of Chagas disease, an infection that can lead to the development of cardiac fibrosis, which is characterized by the deposition of extracellular matrix (ECM) components in the interstitial region of the myocardium. The parasite itself can induce myofibroblast differentiation of cardiac fibroblast in vitro, leading to increased expression of ECM. Inorganic polyphosphate (polyP) is a linear polymer of orthophosphate that can also induce myofibroblast differentiation and deposition of ECM components and is highly abundant in T. cruzi. PolyP can modify proteins post-translationally by non-enzymatic polyphosphorylation of lysine residues of poly-acidic, serine-(S) and lysine (K)-rich (PASK) motifs. In this work, we used a bioinformatics screen and identified the presence of PASK domains in several surface proteins of T. cruzi. We also detected polyP in the external surface of its different life cycle stages and confirmed the stimulation of host cell fibrosis by trypomastigote infection. However, we were not able to detect significant secretion of the polymer or activation of transforming growth factor beta (TGF-β), an important factor for the generation of fibrosis by inorganic polyP- or trypomastigote-conditioned medium.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring the Influence of Hand, Foot, and Mouth Disease: New Guidelines on Patient Care during the 2011–2012 Multiwaves and Multivariant Outbreak in Hai Phong City, Vietnam 监测手足口病的影响：越南海防市 2011-2012 年多波多变疫情期间的患者护理新指南

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-09 DOI: 10.3390/pathogens13090777

Nghia Ngu Duy, Le Thi Thanh Huong, Patrice Ravel, Le Thi Song Huong, Ankit Dwivedi, Guilhem Kister, Laurent Gavotte, Christian A. Devaux, Vu Dinh Thiem, Nguyen Thi Hien Thanh, Tran Nhu Duong, Nguyen Tran Hien, Emmanuel Cornillot, Roger Frutos

{"title":"Monitoring the Influence of Hand, Foot, and Mouth Disease: New Guidelines on Patient Care during the 2011–2012 Multiwaves and Multivariant Outbreak in Hai Phong City, Vietnam","authors":"Nghia Ngu Duy, Le Thi Thanh Huong, Patrice Ravel, Le Thi Song Huong, Ankit Dwivedi, Guilhem Kister, Laurent Gavotte, Christian A. Devaux, Vu Dinh Thiem, Nguyen Thi Hien Thanh, Tran Nhu Duong, Nguyen Tran Hien, Emmanuel Cornillot, Roger Frutos","doi":"10.3390/pathogens13090777","DOIUrl":"https://doi.org/10.3390/pathogens13090777","url":null,"abstract":"From 2011 to 2012, Northern Vietnam suffered its first large-scale hand, foot, and mouth disease (HFMD) epidemic. Two sets of official guidelines were issued during the outbreak to handle the HFMD crisis. The city of Hai Phong was used as a model to analyze the impact of the released guidelines. A total of 9621 HFMD cases were reported in Hai Phong city from April 2011 to December 2012. Three distinct waves of HFMD occurred. Enterovirus A71 and Coxsackievirus A16 were successively associated with the epidemics. Two periods, before and after the guidelines’ release, could be distinguished and characterized by different patient patterns. The time to admission and severity changed notably. Guideline publications help the health system refocus on the 0.5–3 years age group with the highest incidence of the disease. The three waves showed different special distribution, but the main routes of infection were rivers and local secondary roads, most likely through local trade and occupational movements of people.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Genus Ravenelia: Insights on Taxonomy, Diversity and Distribution Ravenelia 属：关于分类、多样性和分布的见解

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-09 DOI: 10.3390/pathogens13090775

Shubhi Avasthi, Ajay Kumar Gautam, Rajnish Kumar Verma, Kunhiraman C. Rajeshkumar, Mekala Niranjan, Amita Sharma, Samantha Chandranath Karunarathna, Nakarin Suwannarach

{"title":"The Genus Ravenelia: Insights on Taxonomy, Diversity and Distribution","authors":"Shubhi Avasthi, Ajay Kumar Gautam, Rajnish Kumar Verma, Kunhiraman C. Rajeshkumar, Mekala Niranjan, Amita Sharma, Samantha Chandranath Karunarathna, Nakarin Suwannarach","doi":"10.3390/pathogens13090775","DOIUrl":"https://doi.org/10.3390/pathogens13090775","url":null,"abstract":"Ravenelia is the third largest rust genus of the order Pucciniales with more than 200 described species. It is an important rust genus that has undergone tremendous taxonomic changes. This genus produces teliospores united into a head on a compound pedicel composed of two to several hyphae with autoecious, macro-, demi- to hemi-, and, more rarely, microcyclic modes of their life cycle which provide it a unique identity and have proved helpful in the identification of the genus. The current understanding of the taxonomy, history, diversity and distribution of this genus is discussed in this paper. Both online and offline resources were searched to gather data of the published literature. The data thus obtained were analyzed for numerical and graphical summaries to provide the diversity and distribution of the genus. In addition, a phylogenetic analysis based on the ITS and nLSU DNA sequence data available in GenBank and the published literature was performed to examine the taxonomic placement of different species within the genus. The genus was reported to be distributed over 53 countries of the world. Around 51 plant genera belonging to four plant families, viz., Fabaceae, Phyllanthaceae, Asphodelaceae and Zygophyllaceae were found to be infected with these rust fungi. The phylogenetic analysis based on LSU and ITS sequence data revealed the polyphyletic nature of the genus. A table of 248 species of this genus is also provided with all information of host, distribution and cited reference that can be helpful for mycologists to find all information at one place. Future perspectives for the advancement of this genus are also discussed.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic and Therapeutic Challenge Caused by Candida albicans and Aspergillus spp. Infections in a Pediatric Patient as a Complication of Acute Lymphoblastic Leukemia Treatment: A Case Report and Literature Review 急性淋巴细胞白血病治疗并发症--一名儿科患者的白色念珠菌和曲霉菌感染所带来的诊断和治疗挑战：病例报告和文献综述

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-07 DOI: 10.3390/pathogens13090772

Natalia Zaj, Weronika Kopyt, Emilia Kamizela, Julia Zarychta, Adrian Kowalczyk, Monika Lejman, Joanna Zawitkowska

{"title":"Diagnostic and Therapeutic Challenge Caused by Candida albicans and Aspergillus spp. Infections in a Pediatric Patient as a Complication of Acute Lymphoblastic Leukemia Treatment: A Case Report and Literature Review","authors":"Natalia Zaj, Weronika Kopyt, Emilia Kamizela, Julia Zarychta, Adrian Kowalczyk, Monika Lejman, Joanna Zawitkowska","doi":"10.3390/pathogens13090772","DOIUrl":"https://doi.org/10.3390/pathogens13090772","url":null,"abstract":"Fungal infections constitute a significant challenge and continue to be a predominant cause of treatment failure in pediatric leukemia cases. Despite the implementation of antifungal prophylaxis, these infections contribute to approximately 20% of cases in children undergoing treatment for acute lymphoblastic leukemia (ALL). The aim of this study is to highlight the diagnostic and therapeutic challenges associated with invasive fungal infections (IFIs). We also present a review of the epidemiology, risk factors, treatment, and a clinical presentation of IFI in patients with ALL. This case report details the clinical course of confirmed Candida albicans (C. albicans) and Aspergillus spp. infections during the consolidation phase of ALL treatment in a 5-year-old pediatric patient. This male patient did not experience any complications until Day 28 of protocol II. Then, the patient’s condition deteriorated. Blood culture detected the growth of C. albicans. Despite the implementation of targeted therapy, the boy’s condition did not show improvement. The appearance of respiratory symptoms necessitated a computed tomography (CT) of the chest, which revealed multiple nodular densities atypical for C. albicans etiology. In spite of ongoing antifungal treatment, the lesions depicted in the CT scans showed no regression. A lung biopsy ultimately identified Aspergillus species as the source of the infection. Overcoming fungal infections poses a considerable challenge; therefore, an accurate diagnosis and the prompt initiation of targeted therapy are crucial in managing these infections in patients with leukemia.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anthrax in Humans, Animals, and the Environment and the One Health Strategies for Anthrax Control 人类、动物和环境中的炭疽以及控制炭疽的统一健康战略

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-07 DOI: 10.3390/pathogens13090773

Deepak Subedi, Saurav Pantha, Sumit Jyoti, Bickal Gautam, Krishna Kaphle, Rakesh Kumar Yadav, Shristi Ghimire, Santosh Dhakal

引用次数: 0

Innovative Multiplex PCR Assay for Detection of tlh, trh, and tdh Genes in Vibrio parahaemolyticus with Reference to the U.S. FDA’s Bacteriological Analytical Manual (BAM) 用于检测副溶血性弧菌中 tlh、trh 和 tdh 基因的创新型多重 PCR 分析法，参照美国 FDA 的《细菌分析手册》（BAM）

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-07 DOI: 10.3390/pathogens13090774

Seong Bin Park, Yan Zhang

{"title":"Innovative Multiplex PCR Assay for Detection of tlh, trh, and tdh Genes in Vibrio parahaemolyticus with Reference to the U.S. FDA’s Bacteriological Analytical Manual (BAM)","authors":"Seong Bin Park, Yan Zhang","doi":"10.3390/pathogens13090774","DOIUrl":"https://doi.org/10.3390/pathogens13090774","url":null,"abstract":"Vibrio parahaemolyticus is an important foodborne bacterium that causes severe gastroenteritis following the consumption of contaminated seafood. To identify V. parahaemolyticus and determine its pathogenicity, the U.S. Food and Drug Administration (FDA)’s Bacteriological Analytical Manual (BAM) recommends a multiplex polymerase chain reaction (PCR) protocol to simultaneously detect the species-specific thermolabile hemolysin (tlh) gene and the pathogenic thermostable-related hemolysin (trh) and thermostable-direct hemolysin (tdh) genes. However, this assay has shown two limitations: difficulty in separating the amplicons of the trh (486 bp) and tlh (450 bp) genes due to their highly similar sizes, and the weaker band exhibited by the tdh gene amplicon (270 bp). The present study aimed to improve the BAM’s multiplex PCR assay by separating the three amplicons with similar intensity. A new primer set was applied for the tlh gene (369 bp) alongside the existing primers for the trh and tdh genes. The amplicons for the three genes were effectively separated by electrophoresis on a 2% tris-borate-EDTA (TBE) agarose gel within 45 min. Primer concentrations of 0.25 µM for three genes produced a significant amount of amplicons among various combinations of primer concentrations with 35 PCR cycles. This assay exhibited a detection limit of 10 pg of bacterial DNA, demonstrating its high sensitivity. It did not display amplicons from nine Vibrio species known to be human pathogens or from 18 well-documented foodborne pathogens. Therefore, the present multiplex PCR protocol could help overcome the limitations of existing assays and provide a more reliable method for detecting the three genes of V. parahaemolyticus.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In the Depths of Wash Water: Isolation of Opportunistic Bacteria from Fresh-Cut Processing Plants 在清洗水的深处：从新鲜水果加工厂分离机会性细菌

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-06 DOI: 10.3390/pathogens13090768

Piotr Kanarek, Barbara Breza-Boruta, Tomasz Bogiel

{"title":"In the Depths of Wash Water: Isolation of Opportunistic Bacteria from Fresh-Cut Processing Plants","authors":"Piotr Kanarek, Barbara Breza-Boruta, Tomasz Bogiel","doi":"10.3390/pathogens13090768","DOIUrl":"https://doi.org/10.3390/pathogens13090768","url":null,"abstract":"The fruit and vegetable industry in post-harvest processing plants is characterized by a substantial consumption of water resources. Wash waters may serve as an environment for the periodic or permanent habitation of microorganisms, particularly if biofilm forms on the inner walls of tanks and flushing channels. Despite the implementation of integrated food safety monitoring systems in numerous countries, foodborne pathogens remain a global public health and food safety concern, particularly for minimally processed food products such as vegetables and fruits. This necessitates the importance of studies that will explore wash water quality to safeguard minimally processed food against foodborne pathogen contamination. Therefore, the current study aimed to isolate and identify bacteria contaminating the wash waters of four fresh-cut processing plants (Poland) and to evaluate the phenotypic antibiotic resistance profiles in selected species. Bacteria were isolated using membrane filtration and identified through mass spectrometry, followed by antibiotic susceptibility testing according to EUCAST guidelines. The results revealed that the level of contamination with total aerobic bacteria in the water ranged from 1.30 × 106 cfu/mL to 2.54 × 108 cfu/mL. Among the isolates, opportunistic pathogens including Enterococcus faecalis, Pseudomonas aeruginosa, Klebsiella oxytoca, Klebsiella pneumoniae, Serratia marcescens, and Proteus vulgaris strains were identified. An especially noteworthy result was the identification of cefepime-resistant K. oxytoca isolates. These findings highlight the importance of monitoring the microbial microflora in minimally processed foods and the need for appropriate sanitary control procedures to minimize the risk of pathogen contamination, ensuring that products remain safe and of high quality throughout the supply chain.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Diversity of Seed-Borne Fungi Associated with Soybean Grown in Southern Poland 波兰南部大豆种子真菌的多样性

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-06 DOI: 10.3390/pathogens13090769

Hanna Olszak-Przybyś, Grażyna Korbecka-Glinka

{"title":"The Diversity of Seed-Borne Fungi Associated with Soybean Grown in Southern Poland","authors":"Hanna Olszak-Przybyś, Grażyna Korbecka-Glinka","doi":"10.3390/pathogens13090769","DOIUrl":"https://doi.org/10.3390/pathogens13090769","url":null,"abstract":"Fungi have the potential to colonize soybean seeds in the field, during their maturation in the pods and after harvest, during storage. The aim of this study was to identify fungi inhabiting soybean seeds after storage with varying germination capacity and to evaluate their chemical composition. The research material consisted of twelve soybean seed lots collected from the fields in southern Poland and stored over winter. The germination percentage of these lots ranged between 20.67% and 81.33%. The seeds were subjected to analyses of the main chemical components and mycological analysis. Fungal isolates were subjected to taxonomic identification using microscopic methods and DNA sequencing (using internal transcribed spacer region and secondary barcoding regions). A total number of 355 fungal isolates from 16 genera were identified, with Aspergillus, Alternaria, and Fusarium being the most common. Species were successfully identified in 94% of isolates. Twelve examined seed lots varied significantly in the number of isolated fungal species (from 1 to 17). Moreover, they also differed in the isolated species composition. Highly significant positive correlation was found between the number of Aspergillus psedudoglaucus isolates and the content of free fatty acids. In turn, the number of Fusarium spp. isolates correlated negatively with protein and nitrogen content. Similarly, highly significant negative correlation was found between the number of all fungal isolates and the 1000-seed weight, indicating that smaller seeds are more vulnerable to fungal infection. The results obtained in this study identify species of fungi which may be responsible for lowering quality of the seeds obtained in southern Poland.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Occurrence of Chlamydia felis in Cats and Dogs in Hungary 匈牙利猫狗中猫衣原体的发生率

IF 3.7 3区医学

Pathogens Pub Date : 2024-09-06 DOI: 10.3390/pathogens13090771

Áron Balázs Ulbert, Hajnalka Juhász, Zsanett Karácsony, Katalin Bencze, Zoltán Deim, Katalin Burián, Gabriella Terhes

{"title":"The Occurrence of Chlamydia felis in Cats and Dogs in Hungary","authors":"Áron Balázs Ulbert, Hajnalka Juhász, Zsanett Karácsony, Katalin Bencze, Zoltán Deim, Katalin Burián, Gabriella Terhes","doi":"10.3390/pathogens13090771","DOIUrl":"https://doi.org/10.3390/pathogens13090771","url":null,"abstract":"The World Health Organization (WHO) estimates that many human infections are zoonoses, creating a worldwide public health challenge. Among Chlamydia species, Chlamydia felis is the leading cause of conjunctivitis in cats and is a prominent zoonotic species. This study aimed to determine the occurrence and risk of chlamydiosis in cats and dogs in Szeged, Hungary, and surrounding areas. The total nucleic acids from conjunctival swab samples of symptomatic and asymptomatic animals were extracted using an automated nucleic acid extraction system. After that, DNA was amplified by pan-chlamydia PCR. Bacterial and fungal cultures were also performed to detect other microorganisms. Of the 93 animals, 32 (34.4%) were positive for pan-chlamydia PCR. The positivity rates were 33.3% (26/78) in cats and 40.0% (6/15) in dogs. Furthermore, the positivity rates were 37.2% (16/43) in the cat shelter, 42.4% (14/33) in the veterinary clinic, and 11.7% (2/17) in household pets. In total, 103 species were identified through culture-based examinations, including 97 (94.2%) bacterial and 6 fungal (5.8%) species. From both human and animal health perspectives, it is essential to have a detailed understanding of the circumstances of chlamydiosis, given the global impact of zoonotic diseases.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0